                         SEQUENCE LISTING

<110>  The Broad Institute, Inc.
       Dana-Farber Cancer Institute, Inc.
 
<120>  THERAPEUTIC TARGETING OF PHOSPHATE DYSREGULATION IN CANCER VIA 
       THE XPR1:KIDINS220 PROTEIN COMPLEX

<130>  BROD-5160WP

<150>  US 63/062,890
<151>  2020-08-07

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  1

Met Leu Val Met Glu Gly Ser Ala Phe Ser Lys Pro Leu Lys Asp Lys 
1               5                   10                  15      


Ile Asn Pro Trp Gly Pro Leu Ile Val Met Gly Ile Leu Val Arg Ala 
            20                  25                  30          


Gly Ala Ser Val Gln Arg Asp Ser Pro His Gln Ile Phe Asn Val Thr 
        35                  40                  45              


Trp Arg Val Thr Asn Leu Met Thr Gly Gln Thr Ala Asn Ala Thr Ser 
    50                  55                  60                  


Leu Leu Gly Thr Met Thr Asp Thr Phe Pro Lys Leu Tyr Phe Asp Leu 
65                  70                  75                  80  


Cys Asp Leu Val Gly Asp Tyr Trp Asp Asp Pro Glu Pro Asp Ile Gly 
                85                  90                  95      


Asp Gly Cys Arg Thr Pro Gly Gly Arg Arg Arg Thr Arg Leu Tyr Asp 
            100                 105                 110         


Phe Tyr Val Cys Pro Gly His Thr Val Pro Ile Gly Cys Gly Gly Pro 
        115                 120                 125             


Gly Glu Gly Tyr Cys Gly Lys Trp Gly Cys Glu Thr Thr Gly Gln Ala 
    130                 135                 140                 


Tyr Trp Lys Pro Ser Ser Ser Trp Asp Leu Ile Ser Leu Lys Arg Gly 
145                 150                 155                 160 


Asn Thr Pro Lys Asp Gln Gly Pro Cys Tyr Asp Ser Ser Val Ser Ser 
                165                 170                 175     


Gly Val Gln Gly Ala Thr Pro Gly Gly Arg Cys Asn Pro Leu Val Leu 
            180                 185                 190         


Glu Phe Thr Asp Ala Gly Arg Lys Ala Ser Trp Asp Ala Pro Lys Val 
        195                 200                 205             


Trp Gly Leu Arg Leu Tyr Arg Ser Thr Gly Ala Asp Pro Val Thr Arg 
    210                 215                 220                 


Phe Ser Leu Thr Arg Gln Val Leu Asn Val Gly Pro Arg Val Pro Ile 
225                 230                 235                 240 


Gly Ser Val Asp Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys 
                245                 250                 255     


Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp 
    290                 295                 300                 


Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe 
305                 310                 315                 320 


Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe 
            340                 345                 350         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys 
        355                 360                 365             


Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys 
    370                 375                 380                 


Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp 
385                 390                 395                 400 


Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys 
                405                 410                 415     


Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser 
            420                 425                 430         


Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr 
        435                 440                 445             


Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser 
    450                 455                 460                 


Leu Ser His Ser Pro Gly Lys 
465                 470     


<210>  2
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic RBD Fc fusion protein

<400>  2

Met Leu Val Met Glu Gly Ser Ala Phe Ser Lys Pro Leu Lys Asp Lys 
1               5                   10                  15      


Ile Asn Pro Trp Gly Pro Leu Ile Val Met Gly Ile Leu Val Arg Ala 
            20                  25                  30          


Gly Ala Ser Val Gln Arg Asp Ser Pro His Gln Ile Phe Asn Val Thr 
        35                  40                  45              


Trp Arg Val Thr Asn Leu Met Thr Gly Gln Thr Ala Asn Ala Thr Ser 
    50                  55                  60                  


Leu Leu Gly Thr Met Thr Asp Thr Phe Pro Lys Leu Tyr Phe Asp Leu 
65                  70                  75                  80  


Cys Asp Leu Val Gly Asp Tyr Trp Asp Asp Pro Glu Pro Asp Ile Gly 
                85                  90                  95      


Asp Gly Cys Arg Thr Pro Gly Gly Arg Arg Arg Thr Arg Leu Tyr Asp 
            100                 105                 110         


Phe Tyr Val Cys Pro Gly His Thr Val Pro Ile Gly Cys Gly Gly Pro 
        115                 120                 125             


Gly Glu Gly Tyr Cys Gly Lys Trp Gly Cys Glu Thr Thr Gly Gln Ala 
    130                 135                 140                 


Tyr Trp Lys Pro Ser Ser Ser Trp Asp Leu Ile Ser Leu Lys Arg Gly 
145                 150                 155                 160 


Asn Thr Pro Lys Asp Gln Gly Pro Cys Tyr Asp Ser Ser Val Ser Ser 
                165                 170                 175     


Gly Val Gln Gly Ala Thr Pro Gly Gly Arg Cys Asn Pro Leu Val Leu 
            180                 185                 190         


Glu Phe Thr Asp Ala Gly Arg Lys Ala Ser Trp Asp Ala Pro Lys Val 
        195                 200                 205             


Trp Gly Leu Arg Leu Tyr Arg Ser Thr Gly Ala Asp Pro Val Thr Arg 
    210                 215                 220                 


Phe Ser Leu Thr Arg Gln Val Leu Asn Val Gly Pro Arg Val Pro Ile 
225                 230                 235                 240 


Gly Ser Val Asp Val Pro Arg Asp Cys Gly Cys Lys Pro Cys Ile Cys 
                245                 250                 255     


Thr Val Pro Glu Val Ser Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
            260                 265                 270         


Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val 
        275                 280                 285             


Asp Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp 
    290                 295                 300                 


Asp Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Phe 
305                 310                 315                 320 


Asn Ser Thr Phe Arg Ser Val Ser Glu Leu Pro Ile Met His Gln Asp 
                325                 330                 335     


Trp Leu Asn Gly Lys Glu Phe Lys Cys Arg Val Asn Ser Ala Ala Phe 
            340                 345                 350         


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Arg Pro Lys 
        355                 360                 365             


Ala Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys 
    370                 375                 380                 


Asp Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp 
385                 390                 395                 400 


Ile Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys 
                405                 410                 415     


Asn Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser 
            420                 425                 430         


Lys Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr 
        435                 440                 445             


Cys Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser 
    450                 455                 460                 


Leu Ser His Ser Pro Gly Lys 
465                 470     


<210>  3
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic N-terminal capping region

<400>  3

Met Asp Pro Ile Arg Ser Arg Thr Pro Ser Pro Ala Arg Glu Leu Leu 
1               5                   10                  15      


Ser Gly Pro Gln Pro Asp Gly Val Gln Pro Thr Ala Asp Arg Gly Val 
            20                  25                  30          


Ser Pro Pro Ala Gly Gly Pro Leu Asp Gly Leu Pro Ala Arg Arg Thr 
        35                  40                  45              


Met Ser Arg Thr Arg Leu Pro Ser Pro Pro Ala Pro Ser Pro Ala Phe 
    50                  55                  60                  


Ser Ala Asp Ser Phe Ser Asp Leu Leu Arg Gln Phe Asp Pro Ser Leu 
65                  70                  75                  80  


Phe Asn Thr Ser Leu Phe Asp Ser Leu Pro Pro Phe Gly Ala His His 
                85                  90                  95      


Thr Glu Ala Ala Thr Gly Glu Trp Asp Glu Val Gln Ser Gly Leu Arg 
            100                 105                 110         


Ala Ala Asp Ala Pro Pro Pro Thr Met Arg Val Ala Val Thr Ala Ala 
        115                 120                 125             


Arg Pro Pro Arg Ala Lys Pro Ala Pro Arg Arg Arg Ala Ala Gln Pro 
    130                 135                 140                 


Ser Asp Ala Ser Pro Ala Ala Gln Val Asp Leu Arg Thr Leu Gly Tyr 
145                 150                 155                 160 


Ser Gln Gln Gln Gln Glu Lys Ile Lys Pro Lys Val Arg Ser Thr Val 
                165                 170                 175     


Ala Gln His His Glu Ala Leu Val Gly His Gly Phe Thr His Ala His 
            180                 185                 190         


Ile Val Ala Leu Ser Gln His Pro Ala Ala Leu Gly Thr Val Ala Val 
        195                 200                 205             


Lys Tyr Gln Asp Met Ile Ala Ala Leu Pro Glu Ala Thr His Glu Ala 
    210                 215                 220                 


Ile Val Gly Val Gly Lys Gln Trp Ser Gly Ala Arg Ala Leu Glu Ala 
225                 230                 235                 240 


Leu Leu Thr Val Ala Gly Glu Leu Arg Gly Pro Pro Leu Gln Leu Asp 
                245                 250                 255     


Thr Gly Gln Leu Leu Lys Ile Ala Lys Arg Gly Gly Val Thr Ala Val 
            260                 265                 270         


Glu Ala Val His Ala Trp Arg Asn Ala Leu Thr Gly Ala Pro Leu Asn 
        275                 280                 285             


<210>  4
<211>  183
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic C-terminal capping region

<400>  4

Arg Pro Ala Leu Glu Ser Ile Val Ala Gln Leu Ser Arg Pro Asp Pro 
1               5                   10                  15      


Ala Leu Ala Ala Leu Thr Asn Asp His Leu Val Ala Leu Ala Cys Leu 
            20                  25                  30          


Gly Gly Arg Pro Ala Leu Asp Ala Val Lys Lys Gly Leu Pro His Ala 
        35                  40                  45              


Pro Ala Leu Ile Lys Arg Thr Asn Arg Arg Ile Pro Glu Arg Thr Ser 
    50                  55                  60                  


His Arg Val Ala Asp His Ala Gln Val Val Arg Val Leu Gly Phe Phe 
65                  70                  75                  80  


Gln Cys His Ser His Pro Ala Gln Ala Phe Asp Asp Ala Met Thr Gln 
                85                  90                  95      


Phe Gly Met Ser Arg His Gly Leu Leu Gln Leu Phe Arg Arg Val Gly 
            100                 105                 110         


Val Thr Glu Leu Glu Ala Arg Ser Gly Thr Leu Pro Pro Ala Ser Gln 
        115                 120                 125             


Arg Trp Asp Arg Ile Leu Gln Ala Ser Gly Met Lys Arg Ala Lys Pro 
    130                 135                 140                 


Ser Pro Thr Ser Thr Gln Thr Pro Asp Gln Ala Ser Leu His Ala Phe 
145                 150                 155                 160 


Ala Asp Ser Leu Glu Arg Asp Leu Asp Ala Pro Ser Pro Met His Glu 
                165                 170                 175     


Gly Asp Gln Thr Arg Ala Ser 
            180             


<210>  5
<211>  7
<212>  PRT
<213>  Simian virus 40

<400>  5

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  6
<211>  10
<212>  PRT
<213>  Simian virus 40

<400>  6

Pro Lys Lys Lys Arg Lys Val Glu Ala Ser 
1               5                   10  


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic nucleoplasmin bipartite NLS

<400>  7

Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys 
1               5                   10                  15      


<210>  8
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  8

Pro Ala Ala Lys Arg Val Lys Leu Asp 
1               5                   


<210>  9
<211>  11
<212>  PRT
<213>  Homo sapiens

<400>  9

Arg Gln Arg Arg Asn Glu Leu Lys Arg Ser Pro 
1               5                   10      


<210>  10
<211>  38
<212>  PRT
<213>  Homo sapiens

<400>  10

Asn Gln Ser Ser Asn Phe Gly Pro Met Lys Gly Gly Asn Phe Gly Gly 
1               5                   10                  15      


Arg Ser Ser Gly Pro Tyr Gly Gly Gly Gly Gln Tyr Phe Ala Lys Pro 
            20                  25                  30          


Arg Asn Gln Gly Gly Tyr 
        35              


<210>  11
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  11

Arg Met Arg Ile Glx Phe Lys Asn Lys Gly Lys Asp Thr Ala Glu Leu 
1               5                   10                  15      


Arg Arg Arg Arg Val Glu Val Ser Val Glu Leu Arg Lys Ala Lys Lys 
            20                  25                  30          


Asp Glu Gln Ile Leu Lys Arg Arg Asn Val 
        35                  40          


<210>  12
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  12

Val Ser Arg Lys Arg Pro Arg Pro 
1               5               


<210>  13
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  13

Pro Pro Lys Lys Ala Arg Glu Asp 
1               5               


<210>  14
<211>  8
<212>  PRT
<213>  Homo sapiens

<400>  14

Pro Gln Pro Lys Lys Lys Pro Leu 
1               5               


<210>  15
<211>  12
<212>  PRT
<213>  Mus sp.

<400>  15

Ser Ala Leu Ile Lys Lys Lys Lys Lys Met Ala Pro 
1               5                   10          


<210>  16
<211>  5
<212>  PRT
<213>  Influenza virus

<400>  16

Asp Arg Leu Arg Arg 
1               5   


<210>  17
<211>  7
<212>  PRT
<213>  Influenza virus

<400>  17

Pro Lys Gln Lys Lys Arg Lys 
1               5           


<210>  18
<211>  10
<212>  PRT
<213>  Hepatitis D virus

<400>  18

Arg Lys Leu Lys Lys Lys Ile Lys Lys Leu 
1               5                   10  


<210>  19
<211>  10
<212>  PRT
<213>  Mus sp.

<400>  19

Arg Glu Lys Lys Lys Phe Leu Lys Arg Arg 
1               5                   10  


<210>  20
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  20

Lys Arg Lys Gly Asp Glu Val Asp Gly Val Asp Glu Val Ala Lys Lys 
1               5                   10                  15      


Lys Ser Lys Lys 
            20  


<210>  21
<211>  17
<212>  PRT
<213>  Homo sapiens

<400>  21

Arg Lys Cys Leu Gln Ala Gly Met Asn Leu Glu Ala Arg Lys Thr Lys 
1               5                   10                  15      


Lys 
    


<210>  22
<211>  1415
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  22
atgctcgtga tggagggttc tgctttcagt aaacccctca aagacaagat caacccatgg       60

ggcccattga ttgtcatggg gattcttgtg agggccgggg caagcgtgca gagagactcc      120

cctcaccaga ttttcaacgt tacatggcgg gtgactaatc ttatgaccgg ccaaacggct      180

aatgccacct ccctgttggg gacaatgact gacaccttcc caaagctcta cttcgacctg      240

tgcgacctgg taggcgacta ttgggacgat cctgaaccag acatcggaga tggttgcaga      300

acacctggag gtcgccgccg cacaagactg tatgacttct acgtctgccc tgggcacacc      360

gtgccaattg ggtgtggcgg cccaggtgag ggatactgcg ggaaatgggg gtgcgaaacc      420

actggtcagg catattggaa gcccagcagt tcctgggatt tgatctctct caaacgaggt      480

aacaccccaa aagaccaagg accctgctat gacagcagtg tcagctccgg agttcagggt      540

gcaactccgg ggggccgatg taacccgctg gtcctggagt ttacagacgc cgggcgcaag      600

gcctcttgga cgctcctaaa gtttggggtt tgaggctgta tcgatcaact ggtgccgatc      660

cagtaactcg gttctcctta acaagacaag tgctcaatgt tggtccccga gtgccaatcg      720

gctccgtaga cgtccccagg gactgtggtt gcaagccctg catatgtacg gttccagaag      780

tcagcagtgt ctttatcttc cccccaaaac caaaagatgt actgaccata acactgactc      840

ccaaggtcac atgcgtggtg gtggacatca gcaaagatga tccagaggtc cagttcagct      900

ggttcgtcga cgatgtggag gtgcatacag cacagaccca gccaagggaa gaacagttca      960

actccacttt ccgctccgtg tctgaattgc ctattatgca tcaggactgg ctgaacggca     1020

aagaattcaa gtgtcgcgtg aattctgccg ccttccctgc tcctatagaa aaaacaatta     1080

gcaagactaa gggccgacct aaggccccgc aggtatacac aattccccca cccaaggagc     1140

agatggcgaa agataaagtc tccttgactt gtatgatcac cgattttttc cctgaagata     1200

ttacggtaga atggcagtgg aacggccagc ctgctgagaa ctacaagaac actcagccta     1260

ttatggatac agacggtagc tacttcgttt acagcaaact caatgtgcag aagtcaaatt     1320

gggaagcagg caacacattc acctgcagtg ttctgcatga gggcttgcat aaccatcaca     1380

cagagaagag cttatcacat tccccgggta aatga                                1415


