                         SEQUENCE LISTING

<110>  Tsinghua University
       ZHU, Ting
       FAN, Chuyao
       DENG, Qiang
       XU, Yuan
 
<120>  CHEMICAL SYNTHESIS OF LARGE AND MIRROR-IMAGE PROTEINS AND USES 
       THEREOF

<130>  87597

<150>  US 63/061,844
<151>  2020-08-06

<160>  116   

<170>  PatentIn version 3.5

<210>  1
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  1
gtaaaacgac ggccagttcg cgcgtttcgg tgatgacggt gaaaaccatt acaataacgt       60

actgcatcca gagttactag atgaaccata tgtacacttg acgttccatc agcctaatca      120

cttcgatgat cctgcgttag actatgtcaa gcagtcatta gatcagctac ctatgacata      180

tgtaccagat gatcactagt atcgtcatag ctgtttcctg                            220


<210>  2
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  2
gtaaaacgac ggccagttct gacacatgca gctcccggag acggtcaatt acccgatcga       60

gccagtcgat cacttcgatg atcacgtagc gtctgctgat gacttcacgt atcagtctaa      120

tctacacgac tcattcaacg tgaatcgact caagttgcca tcagacttag atcagctaga      180

tctgccatat gatctagtcg agtgtcatag ctgtttcctg                            220


<210>  3
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  3
gtaaaacgac ggccagtcag cttgtctgta agcggatgcc gggagcaatt agacattgat       60

gcatccagtc gacgtacgat atgatcagca cttagatcta gatgtactag atgatcagtt      120

caatctgcag ctcaatgcag actagcagtt actccatcac ttcgatgatc cagagttact      180

agactagcac tcatactagc gtcgtcatag ctgtttcctg                            220


<210>  4
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  4
gtaaaacgac ggccagtgac aagcccgtca gggcgcgtca gcgggtcatt aggatgatca       60

tgtgaatgct gatgtacact tagatcagtc taatcacgtg atgaatctga agcgtcagct      120

acgatatcgt aatgagctac gattagatcc gaagtcattg atgcatcacg tagtagcatc      180

tgatgatcac ttcgatgatc agtgtcatag ctgtttcctg                            220


<210>  5
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  5
gtaaaacgac ggccagtttg gcgggtgtcg gggctggctt aactatgatt taagacgaca       60

gttagagcac tatgatcacg tagcgtctgc tgatgacttc acgttctatc atagacatgt      120

catcgacgga ctagatcacg atctacatgc gaatccgaag ttcatgatgc atccgaagtc      180

attgatgcat cgactcaatg taggtcatag ctgtttcctg                            220


<210>  6
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  6
gtaaaacgac ggccagtcgg catcagagca gattgtactg agagtgcatt tatatgtaca       60

ctatcagcac ttagatgtga ctaatcacta gtatcagtca ttcaatcgtc agcatgcgat      120

ctatcatacg atcgagtatc cagagtcatt gatgcatcct aagttcaatg tagatgatga      180

tcacttcgat gatcagttca gatgtcatag ctgtttcctg                            220


<210>  7
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  7
gtaaaacgac ggccagtacc atatgcggtg tgaaataccg cacagatatt ttaacgtaca       60

gctagacgac tagcagttac atcagtctaa tctgaagcgt cagctacgat atcacttcga      120

gctacgatta gatcagccta atccagatgt gatgacattg aagttagatg tccatctcaa      180

gcgattcgac tatcacgtag atcgtcatag ctgtttcctg                            220


<210>  8
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  8
gtaaaacgac ggccagtgcg taaggagaaa ataccgcatc aggcgtgatt ttcagcacta       60

tcagctagtc catcgtaatg cagacgctga tgatctgaat gctaactcac atcagcctaa      120

tcacttcgat gatcacgtga gtacatctga tgtacatcag tctaatcact tcgatgatcg      180

tatgaagtag ttgctccatc tacgtcatag ctgtttcctg                            220


<210>  9
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  9
gtaaaacgac ggccagtatt cgccattcag gctgcgcaac tgttgggatt ttgagtcgaa       60

tctgaatgct aacttccatc gtaatgcaga cgctgatgat ctcaagcgat tcgacttgga      120

tcataacttc gatgtagatg atcacgtcaa tgatcctgcg ttagactatg tcaagcatgt      180

agatccgatc gagccagtcg atcgtcatag ctgtttcctg                            220


<210>  10
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  10
gtaaaacgac ggccagtaag ggcgatcggt gcgggcctct tcgctataca attctacatt       60

catacagcta gtcgatcctg catcagtcga tccgaagttc agcaatccta agttcaatca      120

cttcgatgat cctacatact cattcaatgt ccatcacgta ctgcatctgc atgctgacgt      180

actgcatctc gatgtaccag atggtcatag ctgtttcctg                            220


<210>  11
<211>  220
<212>  DNA
<213>  Artificial sequence

<220>
<223>  L-DNA nucleic acid sequence

<400>  11
gtaaaacgac ggccagttac gccagctggc gaaaggggga tgtgctgaca acactaagtt       60

caacttcgat cacttcgatg atcctgagtt agactatcta gatgtcaagc agtcattaga      120

tccagagtta ctagagctgc atgtcaacga ctagcagtta catcctatca agtctgatct      180

agcagagcat gtaccagatg tctgtcatag ctgtttcctg                            220


<210>  12
<211>  100
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA Barcode nucleic acid sequence

<400>  12
gtaaaacgac ggccagtata tgaagtactc attagatcat agacagttac tgctccatca       60

tagtaatgag caatagctac gatgtcatag ctgtttcctg                            100


<210>  13
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  13
tttgttggag agtttgatcc tggctcaggg tgaacgctgg cggcgtgcct aagacatgca       60

agtcgtgcgg gccgcggggt tttactccgt                                        90


<210>  14
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  14
tttccccggg ttgtccccct cttccgggta ggtcacccac gcgttactca cccgtccgcc       60

gctgaccacg gagtaaaacc ccgcggcccg                                        90


<210>  15
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  15
ggaagagggg gacaacccgg ggaaactcgg gctaatcccc catgtggacc cgccccttgg       60

ggtgtgtcca aagggctttg cccgcttccg                                        90


<210>  16
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  16
cggctacccg tcgtcgcctt ggtgggccat taccccacca actagctgat gggacgcggg       60

cccatccgga agcgggcaaa gccctttgga                                        90


<210>  17
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  17
aaggcgacga cgggtagccg gtctgagagg atggccggcc acaggggcac tgagacacgg       60

gccccactcc tacgggaggc agcagttagg                                        90


<210>  18
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  18
accccgaagg gcttcttcct ccaagcggcg tcgctccgtc aggcttgcgc ccattgcgga       60

agattcctaa ctgctgcctc ccgtaggagt                                        90


<210>  19
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  19
cttggaggaa gaagcccttc ggggtgtaaa ctcctgaacc cgggacgaaa cccccgacga       60

ggggactgac ggtaccgggg taatagcgcc                                        90


<210>  20
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  20
acgcccagtg aatccgggta acgctcgcgc cctccgtatt accgcggctg ctggcacgga       60

gttggccggc gctattaccc cggtaccgtc                                        90


<210>  21
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  21
gcgttacccg gattcactgg gcgtaaaggg cgtgtaggcg gcctggggcg tcccatgtga       60

aagaccacgg ctcaaccgtg ggggagcgtg                                        90


<210>  22
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  22
tatctgcgca tttcaccgct actccgggaa ttccaccacc ctctcccacc gtctagcctg       60

agcgtatccc acgctccccc acggttgagc                                        90


<210>  23
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  23
aattcccgga gtagcggtga aatgcgcaga taccgggagg aacgccgatg gcgaaggcag       60

ccacctggtc cacccgtgac gctgaggcgc                                        90


<210>  24
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  24
agacctagcg cgcatcgttt agggcgtgga ctacccgggt atctaatccg gtttgctccc       60

cacgctttcg cgcctcagcg tcacgggtgg                                        90


<210>  25
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  25
ccctaaacga tgcgcgctag gtctctgggt ctcctggggg ccgaagctaa cgcgttaagc       60

gcgccgcctg gggagtacgg ccgcaaggct                                        90


<210>  26
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  26
ttcgcgttgc ttcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattcc       60

tttgagtttc agccttgcgg ccgtactccc                                        90


<210>  27
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  27
ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggccttg acatgctagg       60

gaacccgggt gaaagcctgg ggtgccccgc                                        90


<210>  28
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  28
ggacttaacc caacacctca cggcacgagc tgacgacggc catgcagcac ctgtgctagg       60

gctcccctcg cggggcaccc caggctttca                                        90


<210>  29
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  29
cgtgccgtga ggtgttgggt taagtcccgc aacgagcgca acccccgccg ttagttgcca       60

gcggttcggc cgggcactct aacgggactg                                        90


<210>  30
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  30
tgtgtcgccc aggccgtaag ggccatgctg accagacgtc gtcccctcct tcctcccgct       60

ttcgcgggca gtcccgttag agtgcccggc                                        90


<210>  31
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  31
ggcccttacg gcctgggcga cacacgtgct acaatgccca ctacaaagcg atgccacccg       60

gcaacgggga gctaatcgca aaaaggtggg                                        90


<210>  32
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  32
gatccgcgat tactagcgat tccggcttca tggggtcggg ttgcagaccc caatccgaac       60

tgggcccacc tttttgcgat tagctccccg                                        90


<210>  33
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  33
gccggaatcg ctagtaatcg cggatcagcc atgccgcggt gaatacgttc ccgggccttg       60

tacacaccgc ccgtcacgcc atgggagcgg                                        90


<210>  34
<211>  90
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  34
cgacttcgcc ccagtcacgg gccctaccct cggcgcctgc ccgtaggctc ccggcgactt       60

cgggtagagc ccgctcccat ggcgtgacgg                                        90


<210>  35
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  35
ccgcaccttc cggtacagct accttgttac gacttcgccc cagtcacggg ccct             54


<210>  36
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  36
gtaaaacgac ggccagt                                                      17


<210>  37
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  37
caggaaacag ctatgac                                                      17


<210>  38
<211>  54
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  38
gtgctgcaag gcgattaatt aggtatacaa ccagaaccag attaagattg tata             54


<210>  39
<211>  69
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic oligo nucleic acid sequence

<400>  39
ctatgactgt taacctatac aatcttaatc tggttctggt tgtataccta attaatcgcc       60

ttgcagcac                                                               69


<210>  40
<211>  113
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic D-/L- chimeric oligo nucleic acid sequence

<400>  40
ggttaacagt catagctgtt tcctggtaaa acgacggcca gtattacctt aacaacctat       60

accacatata ccaggttcag attctatagg ttcacagtca tagctgtttc ctg             113


<210>  41
<211>  98
<212>  DNA
<213>  Artificial sequence

<220>
<223>  short synthetic D-/L- chimeric oligo nucleic acid sequence

<400>  41
caggaaacag ctatgactgt gaacctatag aatctgaacc tggtatatgt ggtataggtt       60

gttaaggtaa tactggccgt cgttttacca ggaaacag                               98


<210>  42
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  42
gtgctgcaag gcgatta                                                      17


<210>  43
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand DNA oligonucleotide

<400>  43
caggaaacag ctatgac                                                      17


<210>  44
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand L-DNA oligonucleotide

<400>  44
gtaaaacgac ggccagt                                                      17


<210>  45
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Single strand L-DNA oligonucleotide

<400>  45
caggaaacag ctatgac                                                      17


<210>  46
<211>  167
<212>  DNA
<213>  Artificial sequence

<220>
<223>  D-/L- chimeric DNA nucleic acid sequence

<400>  46
gtgctgcaag gcgattaatt aggtatacaa ccagaaccag attaagattg tataggttaa       60

cagtcatagc tgtttcctgg taaaacgacg gccagtatta ccttaacaac ctataccaca      120

tataccaggt tcagattcta taggttcaca gtcatagctg tttcctg                    167


<210>  47
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase

<400>  47

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Glu His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  48
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mutant version of the Pfu DNA polymerase

<400>  48

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Ala Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Gly Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  49
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  XXXXX

<400>  49

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Ala Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Gly Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Val Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Val Asp Glu Glu Gly Lys Val Thr Thr Arg Gly 
        595                 600                 605             


Leu Glu Val Val Arg Arg Asp Trp Ser Glu Ala Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Leu Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Val Val Lys Glu Val Thr Gln Lys Leu Ala Asn Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Tyr Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Val Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Val Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Pro Lys Lys Ser 
    770                 775 


<210>  50
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  XXXXX

<400>  50

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Phe Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Val Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Val 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ala Thr Trp Lys Asn Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Val Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Arg Glu Lys Asp Pro Asp Val Leu Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Val Lys Leu Thr Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Met Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Val 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ala Thr Arg Thr Leu Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Ala Ala Val Tyr Glu Ala Val Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Gly Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Val Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Val Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  51
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mutant version of the Pfu DNA polymerase

<400>  51

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Phe Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Val Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Val 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ala Thr Trp Lys Asn Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Val Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Arg Glu Lys Asp Pro Asp Val Leu Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Val Lys Leu Thr Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Met Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Val 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ala Thr Arg Thr Leu Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Ala Ala Val Tyr Glu Ala Val Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Gly Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Val Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Val Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Val Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Val Asp Glu Glu Gly Lys Val Thr Thr Arg Gly 
        595                 600                 605             


Leu Glu Val Val Arg Arg Asp Trp Ser Glu Ala Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Leu Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Val Val Lys Glu Val Thr Gln Lys Leu Ala Asn Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Tyr Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Val Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Val Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Pro Lys Lys Ser 
    770                 775 


<210>  52
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mutant version of the Pfu DNA polymerase

<400>  52

Met Val Leu Asp Val Asp Tyr Leu Thr Glu Glu Gly Lys Pro Val Val 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Val Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Phe Tyr Ala Leu Leu Arg Asp Asp Ser Lys Leu 
        35                  40                  45              


Glu Glu Val Lys Lys Val Thr Gly Glu Arg His Gly Lys Val Val Arg 
    50                  55                  60                  


Val Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Val 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Leu 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Val Phe Glu Tyr 
            100                 105                 110         


Asp Val Pro Phe Ala Lys Arg Tyr Leu Val Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Met Leu Ala Phe Asp Val Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Val Leu Met Val 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ala Thr Trp Lys Asn Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Val Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Arg Glu Lys Asp Pro Asp Val Leu Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Val Lys Leu Thr Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Met Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Val 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ala Thr Arg Thr Leu Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Ala Ala Val Tyr Glu Ala Val Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Val Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Gly Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Val Val Tyr Leu Asp Phe Arg Ala Leu Tyr Pro Ser Val Val Val Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Val Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly 
        435                 440                 445             


Phe Val Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Val 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Val Glu Lys Lys Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Val Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Val Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Val Pro Gly Gly Glu Ser Glu Glu Val Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Val Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Val Asp Glu Glu Gly Lys Val Thr Thr Arg Gly 
        595                 600                 605             


Leu Glu Val Val Arg Arg Asp Trp Ser Glu Ala Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Leu Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Val Val Lys Glu Val Thr Gln Lys Leu Ala Asn Tyr Glu Val 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Val Tyr Glu Gln Val Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Val Lys Pro Gly Met Val Val Gly Tyr Val Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Val Ser Asn Arg Ala Tyr Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Val Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Val Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Pro Lys Lys Ser 
    770                 775 


<210>  53
<211>  774
<212>  PRT
<213>  Artificial sequence

<220>
<223>  KOD1 polymerase

<400>  53

Met Ile Leu Asp Thr Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Glu Pro Tyr Phe Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Ala Glu Arg His Gly Thr Val Val Thr 
    50                  55                  60                  


Val Lys Arg Val Glu Lys Val Gln Lys Lys Phe Leu Gly Arg Pro Val 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Thr His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Ile Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr Glu Glu Gly Glu Glu Phe Ala Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Arg Val Ile Thr Trp Lys Asn Val 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Lys Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Cys Glu 
    210                 215                 220                 


Lys Leu Gly Ile Asn Phe Ala Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Val Phe Gly Gln Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Thr Thr Ala Trp Glu Thr Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Ile Gly Gln Ser Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Lys Glu Leu Ala Arg Arg Arg Gln Ser Tyr 
    370                 375                 380                 


Glu Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Ile Lys 
    450                 455                 460                 


Lys Lys Met Lys Ala Thr Ile Asp Pro Ile Glu Arg Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Arg Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Glu Tyr Ile Thr Met Thr Ile Lys Glu Ile 
        515                 520                 525             


Glu Glu Lys Tyr Gly Phe Lys Val Ile Tyr Ser Asp Thr Asp Gly Phe 
    530                 535                 540                 


Phe Ala Thr Ile Pro Gly Ala Asp Ala Glu Thr Val Lys Lys Lys Ala 
545                 550                 555                 560 


Met Glu Phe Leu Lys Tyr Ile Asn Ala Lys Leu Pro Gly Ala Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Glu Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Gly Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Leu Leu Lys Asp Gly Asp Val Glu Lys Ala Val 
625                 630                 635                 640 


Arg Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile His Glu Gln Ile Thr Arg Asp Leu Lys Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Val Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Thr Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Arg Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Ser Ala Trp 
        755                 760                 765             


Leu Lys Pro Lys Gly Thr 
    770                 


<210>  54
<211>  773
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Tgo polymerase

<400>  54

Met Ile Leu Asp Thr Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Ile Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Asp Tyr Asp Arg 
            20                  25                  30          


Asn Phe Glu Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Asp Val Lys Lys Ile Thr Ala Glu Arg His Gly Thr Thr Val Arg 
    50                  55                  60                  


Val Val Arg Ala Glu Lys Val Lys Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Thr His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Lys Glu His Pro Ala Val Val Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Lys Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Ala Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Arg Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Lys Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Val Lys Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Ser Glu 
    210                 215                 220                 


Lys Leu Gly Val Lys Phe Ile Leu Gly Arg Glu Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Gln Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Ala Gln Ala Trp Glu Thr Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Ser Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Leu Ala Arg Arg Arg Glu Ser Tyr 
    370                 375                 380                 


Ala Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Glu Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Val Lys 
    450                 455                 460                 


Lys Lys Met Lys Ala Thr Ile Asp Pro Ile Glu Lys Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Gln Tyr Ile Glu Thr Thr Ile Arg Glu Ile 
        515                 520                 525             


Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly Phe 
    530                 535                 540                 


Phe Ala Thr Ile Pro Gly Ala Asp Ala Glu Thr Val Lys Lys Lys Ala 
545                 550                 555                 560 


Lys Glu Phe Leu Asp Tyr Ile Asn Ala Lys Leu Pro Gly Leu Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Asp Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Ile Leu Lys His Gly Asp Val Glu Glu Ala Val 
625                 630                 635                 640 


Arg Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Asp Leu Lys Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Ile Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Ala Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Arg Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Lys Pro Lys Thr 
    770             


<210>  55
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  9 degrees N-7 polymerase amino acid sequence

<400>  55

Met Ile Leu Asp Thr Asp Tyr Ile Thr Glu Asn Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Val Phe Lys Lys Glu Asn Gly Glu Phe Lys Ile Glu Tyr Asp Arg 
            20                  25                  30          


Thr Phe Glu Pro Tyr Phe Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Asp Val Lys Lys Val Thr Ala Lys Arg His Gly Thr Val Val Lys 
    50                  55                  60                  


Val Lys Arg Ala Glu Lys Val Gln Lys Lys Phe Leu Gly Arg Pro Ile 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Asn His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Arg Ile Arg Ala His Pro Ala Val Val Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Thr Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Thr Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Gly Ser Glu Ala Arg Val Ile Thr Trp Lys Lys Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Asp Val Val Ser Thr Glu Lys Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Val Val Arg Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Cys Glu 
    210                 215                 220                 


Glu Leu Gly Ile Lys Phe Thr Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Val Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Ala Gln Ala Trp Glu Ser Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Val Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Arg Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Ile Gly Gln Ser Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Lys Arg Asn Glu Leu Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Leu Ala Arg Arg Arg Gly Gly Tyr 
    370                 375                 380                 


Ala Gly Gly Tyr Val Lys Glu Pro Glu Arg Gly Leu Trp Asp Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Phe Arg Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Lys Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Glu Val Gly His Lys Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Ile Lys 
    450                 455                 460                 


Arg Lys Met Lys Ala Thr Val Asp Pro Leu Glu Lys Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Phe Tyr Gly Tyr 
                485                 490                 495     


Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Glu Tyr Ile Glu Met Val Ile Arg Glu Leu 
        515                 520                 525             


Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly Leu 
    530                 535                 540                 


His Ala Thr Ile Pro Gly Ala Asp Ala Glu Thr Val Lys Lys Lys Ala 
545                 550                 555                 560 


Lys Glu Phe Leu Lys Tyr Ile Asn Pro Lys Leu Pro Gly Leu Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Val Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Gly Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Ile Leu Lys His Gly Asp Val Glu Glu Ala Val 
625                 630                 635                 640 


Arg Ile Val Lys Glu Val Thr Glu Lys Leu Ser Lys Tyr Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile His Glu Gln Ile Thr Arg Asp Leu Arg Asp 
            660                 665                 670         


Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Val Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Ser Gly Arg Ile Gly Asp Arg Ala Ile Pro Ala Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Thr Lys His Arg Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Lys Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Arg Tyr Gln Lys Thr Lys Gln Val Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Lys Val Lys Gly Lys Lys 
    770                 775 


<210>  56
<211>  773
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Tok polymerase

<400>  56

Met Ile Leu Asp Ala Asp Tyr Ile Thr Glu Asp Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Val Phe Lys Lys Glu Lys Gly Glu Phe Lys Ile Asp Tyr Asp Arg 
            20                  25                  30          


Asp Phe Glu Pro Tyr Ile Tyr Ala Leu Leu Lys Asp Asp Ser Ala Ile 
        35                  40                  45              


Glu Asp Ile Lys Lys Ile Thr Ala Glu Arg His Gly Thr Thr Val Arg 
    50                  55                  60                  


Val Thr Arg Ala Glu Arg Val Lys Lys Lys Phe Leu Gly Arg Pro Val 
65                  70                  75                  80  


Glu Val Trp Lys Leu Tyr Phe Thr His Pro Gln Asp Val Pro Ala Ile 
                85                  90                  95      


Arg Asp Lys Ile Arg Glu His Pro Ala Val Val Asp Ile Tyr Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Arg Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Asp Glu Glu Leu Arg Met Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Glu Gly Pro Ile Leu Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Glu Gly Ala Arg Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Ser Val Ser Thr Glu Lys Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Lys Val Ile Gln Glu Lys Asp Pro Asp Val Leu Ile Thr 
        195                 200                 205             


Tyr Asn Gly Asp Asn Phe Asp Phe Ala Tyr Leu Lys Lys Arg Ser Glu 
    210                 215                 220                 


Met Leu Gly Val Lys Phe Ile Leu Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Ile Gln Arg Met Gly Asp Arg Phe Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr Pro Val Ile Arg Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Thr Val Tyr Glu Pro Val Phe Gly Gln Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Glu Glu Ile Ala Arg Ala Trp Glu Ser Gly Glu Gly 
    290                 295                 300                 


Leu Glu Arg Val Ala Arg Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Phe Pro Met Glu Ala Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Ser Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Asp Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Asp Glu Arg Glu Leu Ala Arg Arg Thr Glu Ser Tyr 
    370                 375                 380                 


Ala Gly Gly Tyr Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn Ile 
385                 390                 395                 400 


Val Tyr Leu Asp Tyr Lys Ser Leu Tyr Pro Ser Ile Ile Ile Thr His 
                405                 410                 415     


Asn Val Ser Pro Asp Thr Leu Asn Arg Glu Gly Cys Arg Glu Tyr Asp 
            420                 425                 430         


Val Ala Pro Gln Val Gly His Arg Phe Cys Lys Asp Phe Pro Gly Phe 
        435                 440                 445             


Ile Pro Ser Leu Leu Gly Asp Leu Leu Glu Glu Arg Gln Lys Val Lys 
    450                 455                 460                 


Lys Lys Met Lys Ala Thr Val Asp Pro Ile Glu Arg Lys Leu Leu Asp 
465                 470                 475                 480 


Tyr Arg Gln Arg Ala Ile Lys Ile Leu Ala Asn Ser Tyr Tyr Gly Tyr 
                485                 490                 495     


Tyr Ala Tyr Ala Asn Ala Arg Trp Tyr Cys Arg Glu Cys Ala Glu Ser 
            500                 505                 510         


Val Thr Ala Trp Gly Arg Gln Tyr Ile Glu Thr Thr Met Arg Glu Ile 
        515                 520                 525             


Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly Phe 
    530                 535                 540                 


Phe Ala Thr Ile Pro Gly Ala Asp Ala Glu Thr Val Lys Asn Lys Ala 
545                 550                 555                 560 


Lys Glu Phe Leu Asn Tyr Ile Asn Pro Arg Leu Pro Gly Leu Leu Glu 
                565                 570                 575     


Leu Glu Tyr Glu Gly Phe Tyr Arg Arg Gly Phe Phe Val Thr Lys Lys 
            580                 585                 590         


Lys Tyr Ala Val Ile Asp Glu Glu Asp Lys Ile Thr Thr Arg Gly Leu 
        595                 600                 605             


Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln Ala 
    610                 615                 620                 


Arg Val Leu Glu Ala Ile Leu Lys His Gly Asp Val Glu Glu Ala Val 
625                 630                 635                 640 


Arg Ile Val Lys Glu Val Thr Glu Lys Leu Ser Arg His Glu Val Pro 
                645                 650                 655     


Pro Glu Lys Leu Val Ile Tyr Glu Gln Ile Thr Arg Asp Leu Arg Ser 
            660                 665                 670         


Tyr Arg Ala Thr Gly Pro His Val Ala Val Ala Lys Arg Leu Ala Ala 
        675                 680                 685             


Arg Gly Ile Lys Ile Arg Pro Gly Thr Val Ile Ser Tyr Ile Val Leu 
    690                 695                 700                 


Lys Gly Pro Gly Arg Val Gly Asp Arg Ala Ile Pro Phe Asp Glu Phe 
705                 710                 715                 720 


Asp Pro Ala Lys His Arg Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn Gln 
                725                 730                 735     


Val Leu Pro Ala Val Glu Arg Ile Leu Arg Ala Phe Gly Tyr Arg Lys 
            740                 745                 750         


Glu Asp Leu Arg Tyr Gln Lys Thr Lys Gln Ala Gly Leu Gly Ala Trp 
        755                 760                 765             


Leu Lys Pro Lys Thr 
    770             


<210>  57
<211>  473
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment

<400>  57

His His His His His His Met Ile Leu Asp Val Asp Tyr Ile Thr Glu 
1               5                   10                  15      


Glu Gly Lys Pro Val Ile Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe 
            20                  25                  30          


Lys Ile Glu His Asp Arg Thr Phe Arg Pro Tyr Phe Tyr Ala Leu Leu 
        35                  40                  45              


Arg Asp Asp Ser Lys Ile Glu Glu Val Lys Lys Ile Thr Gly Glu Arg 
    50                  55                  60                  


His Gly Lys Val Val Arg Val Val Asp Val Glu Lys Val Glu Lys Lys 
65                  70                  75                  80  


Phe Leu Gly Lys Pro Val Thr Val Trp Lys Leu Tyr Leu Glu His Pro 
                85                  90                  95      


Gln Asp Val Pro Thr Ile Arg Glu Lys Val Arg Ala His Pro Ala Val 
            100                 105                 110         


Val Asp Ile Phe Glu Tyr Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile 
        115                 120                 125             


Asp Lys Gly Leu Val Pro Met Glu Gly Glu Glu Glu Leu Lys Met Leu 
    130                 135                 140                 


Ala Phe Asp Ile Glu Thr Leu Tyr His Glu Gly Glu Glu Phe Gly Lys 
145                 150                 155                 160 


Gly Pro Ile Leu Met Ile Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val 
                165                 170                 175     


Ala Thr Trp Lys Asn Val Asp Leu Pro Tyr Val Glu Val Val Ser Ser 
            180                 185                 190         


Glu Arg Glu Met Val Lys Arg Phe Leu Arg Val Val Arg Glu Lys Asp 
        195                 200                 205             


Pro Asp Val Leu Val Thr Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr 
    210                 215                 220                 


Leu Ala Lys Arg Ala Glu Lys Leu Gly Val Lys Leu Thr Leu Gly Arg 
225                 230                 235                 240 


Asp Gly Ser Glu Pro Lys Met Gln Arg Met Gly Asp Met Thr Ala Val 
                245                 250                 255     


Glu Val Lys Gly Arg Val His Phe Asp Leu Tyr His Val Ala Thr Arg 
            260                 265                 270         


Thr Leu Asn Leu Pro Thr Tyr Thr Leu Ala Ala Val Tyr Glu Ala Val 
        275                 280                 285             


Phe Gly Lys Pro Lys Glu Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala 
    290                 295                 300                 


Trp Glu Ser Gly Glu Asn Leu Glu Arg Val Ala Lys Tyr Ser Met Glu 
305                 310                 315                 320 


Asp Ala Gly Ala Thr Tyr Glu Leu Gly Lys Glu Phe Leu Pro Met Glu 
                325                 330                 335     


Ala Gln Leu Ser Arg Leu Val Gly Gln Pro Leu Trp Asp Val Ser Arg 
            340                 345                 350         


Ser Ser Thr Gly Asn Leu Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr 
        355                 360                 365             


Glu Arg Asn Glu Leu Ala Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln 
    370                 375                 380                 


Arg Arg Leu Arg Glu Ser Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu 
385                 390                 395                 400 


Lys Gly Leu Trp Glu Asn Val Val Tyr Leu Asp Phe Arg Ala Leu Tyr 
                405                 410                 415     


Pro Ser Ile Ile Ile Thr His Asn Val Ser Pro Asp Thr Leu Asn Leu 
            420                 425                 430         


Glu Gly Cys Lys Asn Tyr Asp Val Ala Pro Gln Val Gly His Lys Phe 
        435                 440                 445             


Cys Lys Asp Phe Pro Gly Phe Ile Pro Ser Leu Leu Gly His Leu Leu 
    450                 455                 460                 


Glu Glu Arg Gln Lys Ile Lys Thr Lys 
465                 470             


<210>  58
<211>  45
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment

<400>  58

His His His His His His Met Ile Leu Asp Val Asp Tyr Ile Thr Glu 
1               5                   10                  15      


Glu Gly Lys Pro Val Ile Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe 
            20                  25                  30          


Lys Ile Glu His Asp Arg Thr Phe Arg Pro Tyr Phe Tyr 
        35                  40                  45  


<210>  59
<211>  62
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment

<400>  59

Cys Leu Leu Arg Asp Asp Ser Lys Ile Glu Glu Val Lys Lys Ile Thr 
1               5                   10                  15      


Gly Glu Arg His Gly Lys Val Val Arg Val Val Asp Val Glu Lys Val 
            20                  25                  30          


Glu Lys Lys Phe Leu Gly Lys Pro Val Thr Val Trp Lys Leu Tyr Leu 
        35                  40                  45              


Glu His Pro Gln Asp Val Pro Thr Ile Arg Glu Lys Val Arg 
    50                  55                  60          


<210>  60
<211>  60
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  60

His Pro Ala Val Val Asp Ile Phe Glu Tyr Asp Ile Pro Phe Ala Lys 
1               5                   10                  15      


Arg Tyr Leu Ile Asp Lys Gly Leu Val Pro Met Glu Gly Glu Glu Glu 
            20                  25                  30          


Leu Lys Met Leu Ala Phe Asp Ile Glu Thr Leu Tyr His Glu Gly Glu 
        35                  40                  45              


Glu Phe Gly Lys Gly Pro Ile Leu Met Ile Ser Tyr 
    50                  55                  60  


<210>  61
<211>  59
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  61

Asp Glu Asn Glu Ala Lys Val Ala Thr Trp Lys Asn Val Asp Leu Pro 
1               5                   10                  15      


Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Val Lys Arg Phe Leu 
            20                  25                  30          


Arg Val Val Arg Glu Lys Asp Pro Asp Val Leu Val Thr Tyr Asn Gly 
        35                  40                  45              


Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg 
    50                  55                  


<210>  62
<211>  53
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment

<400>  62

Cys Glu Lys Leu Gly Val Lys Leu Thr Leu Gly Arg Asp Gly Ser Glu 
1               5                   10                  15      


Pro Lys Met Gln Arg Met Gly Asp Met Thr Ala Val Glu Val Lys Gly 
            20                  25                  30          


Arg Val His Phe Asp Leu Tyr His Val Ala Thr Arg Thr Leu Asn Leu 
        35                  40                  45              


Pro Thr Tyr Thr Leu 
    50              


<210>  63
<211>  41
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  63

Ala Val Tyr Glu Ala Val Phe Gly Lys Pro Lys Glu Lys Val Tyr Ala 
1               5                   10                  15      


Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn Leu Glu Arg Val 
            20                  25                  30          


Ala Lys Tyr Ser Met Glu Asp Ala Gly 
        35                  40      


<210>  64
<211>  49
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  64

Thr Tyr Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ala Gln Leu Ser 
1               5                   10                  15      


Arg Leu Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly 
            20                  25                  30          


Asn Leu Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu 
        35                  40                  45              


Leu 
    


<210>  65
<211>  39
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  65

Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
1               5                   10                  15      


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
            20                  25                  30          


Val Val Tyr Leu Asp Phe Arg 
        35                  


<210>  66
<211>  60
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase N fragment

<400>  66

Cys Leu Tyr Pro Ser Ile Ile Ile Thr His Asn Val Ser Pro Asp Thr 
1               5                   10                  15      


Leu Asn Leu Glu Gly Cys Lys Asn Tyr Asp Val Ala Pro Gln Val Gly 
            20                  25                  30          


His Lys Phe Cys Lys Asp Phe Pro Gly Phe Ile Pro Ser Leu Leu Gly 
        35                  40                  45              


His Leu Leu Glu Glu Arg Gln Lys Ile Lys Thr Lys 
    50                  55                  60  


<210>  67
<211>  308
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment

<400>  67

Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Lys Leu Leu Asp Tyr Arg 
1               5                   10                  15      


Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly Tyr Tyr Gly 
            20                  25                  30          


Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser Val Thr 
        35                  40                  45              


Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu Leu Glu Glu 
    50                  55                  60                  


Lys Phe Gly Phe Lys Val Leu Tyr Ala Asp Thr Asp Gly Leu Tyr Ala 
65                  70                  75                  80  


Thr Ile Pro Gly Gly Glu Ser Glu Glu Val Lys Lys Lys Ala Leu Glu 
                85                  90                  95      


Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu Glu Leu Glu 
            100                 105                 110         


Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys Lys Arg Tyr 
        115                 120                 125             


Ala Val Val Asp Glu Glu Gly Lys Val Thr Thr Arg Gly Leu Glu Val 
    130                 135                 140                 


Val Arg Arg Asp Trp Ser Glu Ala Ala Lys Glu Thr Gln Ala Arg Val 
145                 150                 155                 160 


Leu Glu Thr Leu Leu Lys His Gly Asp Val Glu Glu Ala Val Arg Val 
                165                 170                 175     


Val Lys Glu Val Thr Gln Lys Leu Ala Asn Tyr Glu Val Pro Pro Glu 
            180                 185                 190         


Lys Leu Ala Ile Tyr Glu Gln Ile Thr Arg Pro Leu His Glu Tyr Lys 
        195                 200                 205             


Ala Thr Gly Pro His Val Ala Val Ala Lys Lys Leu Ala Ala Lys Gly 
    210                 215                 220                 


Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val Leu Arg Gly 
225                 230                 235                 240 


Asp Gly Pro Ile Ser Asn Arg Ala Tyr Leu Ala Glu Glu Tyr Asp Pro 
                245                 250                 255     


Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Val Glu Asn Gln Val Leu 
            260                 265                 270         


Pro Ala Val Leu Arg Val Leu Glu Gly Phe Gly Tyr Arg Lys Glu Asp 
        275                 280                 285             


Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser Trp Leu Asn 
    290                 295                 300                 


Pro Lys Lys Ser 
305             


<210>  68
<211>  33
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment

<400>  68

Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Lys Leu Leu Asp Tyr Arg 
1               5                   10                  15      


Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly Tyr Tyr Gly 
            20                  25                  30          


Tyr 
    


<210>  69
<211>  39
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment

<400>  69

Cys Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu Ser Val Thr Ala 
1               5                   10                  15      


Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu Leu Glu Glu Lys 
            20                  25                  30          


Phe Gly Phe Lys Val Leu Tyr 
        35                  


<210>  70
<211>  56
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment

<400>  70

Cys Asp Thr Asp Gly Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu 
1               5                   10                  15      


Glu Val Lys Lys Lys Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys 
            20                  25                  30          


Leu Pro Gly Leu Leu Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly 
        35                  40                  45              


Phe Phe Val Thr Lys Lys Arg Tyr 
    50                  55      


<210>  71
<211>  55
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  71

Val Val Asp Glu Glu Gly Lys Val Thr Thr Arg Gly Leu Glu Val Val 
1               5                   10                  15      


Arg Arg Asp Trp Ser Glu Ala Ala Lys Glu Thr Gln Ala Arg Val Leu 
            20                  25                  30          


Glu Thr Leu Leu Lys His Gly Asp Val Glu Glu Ala Val Arg Val Val 
        35                  40                  45              


Lys Glu Val Thr Gln Lys Leu 
    50                  55  


<210>  72
<211>  62
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  72

Asn Tyr Glu Val Pro Pro Glu Lys Leu Ala Ile Tyr Glu Gln Ile Thr 
1               5                   10                  15      


Arg Pro Leu His Glu Tyr Lys Ala Thr Gly Pro His Val Ala Val Ala 
            20                  25                  30          


Lys Lys Leu Ala Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile 
        35                  40                  45              


Gly Tyr Ile Val Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg 
    50                  55                  60          


<210>  73
<211>  61
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Pfu DNA polymerase C fragment

<400>  73

Cys Tyr Leu Ala Glu Glu Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala 
1               5                   10                  15      


Glu Tyr Tyr Val Glu Asn Gln Val Leu Pro Ala Val Leu Arg Val Leu 
            20                  25                  30          


Glu Gly Phe Gly Tyr Arg Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg 
        35                  40                  45              


Gln Val Gly Leu Thr Ser Trp Leu Asn Pro Lys Lys Ser 
    50                  55                  60      


<210>  74
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mutant version of the Pfu DNA polymerase

<400>  74

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Val Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Gly Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Ala Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Lys Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  75
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mutant version of the Pfu DNA polymerase

<400>  75

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Gln Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Asp Ile Glu Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Gly Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Leu Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Lys Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  76
<211>  775
<212>  PRT
<213>  Artificial sequence

<220>
<223>  mutant version of the Pfu DNA polymerase

<400>  76

Met Ile Leu Asp Val Asp Tyr Ile Thr Glu Glu Gly Lys Pro Val Ile 
1               5                   10                  15      


Arg Leu Phe Lys Lys Glu Asn Gly Lys Phe Lys Ile Glu His Asp Arg 
            20                  25                  30          


Thr Phe Arg Pro Tyr Ile Tyr Ala Leu Leu Arg Asp Asp Ser Lys Ile 
        35                  40                  45              


Glu Glu Val Lys Lys Ile Thr Gly Glu Arg His Gly Lys Ile Val Arg 
    50                  55                  60                  


Ile Val Asp Val Glu Lys Val Glu Lys Lys Phe Leu Gly Lys Pro Ile 
65                  70                  75                  80  


Thr Val Trp Lys Leu Tyr Leu Glu His Pro Gln Asp Gln Pro Thr Ile 
                85                  90                  95      


Arg Glu Lys Val Arg Ala His Pro Ala Val Val Asp Ile Phe Glu Tyr 
            100                 105                 110         


Asp Ile Pro Phe Ala Lys Arg Tyr Leu Ile Asp Lys Gly Leu Ile Pro 
        115                 120                 125             


Met Glu Gly Glu Glu Glu Leu Lys Ile Leu Ala Phe Ala Ile Ala Thr 
    130                 135                 140                 


Leu Tyr His Glu Gly Glu Glu Phe Gly Lys Gly Pro Ile Ile Met Ile 
145                 150                 155                 160 


Ser Tyr Ala Asp Glu Asn Glu Ala Lys Val Ile Thr Trp Lys Asn Ile 
                165                 170                 175     


Asp Leu Pro Tyr Val Glu Val Val Ser Ser Glu Arg Glu Met Ile Lys 
            180                 185                 190         


Arg Phe Leu Arg Ile Ile Arg Glu Lys Asp Pro Asp Ile Ile Val Thr 
        195                 200                 205             


Tyr Asn Gly Asp Ser Phe Asp Phe Pro Tyr Leu Ala Lys Arg Ala Glu 
    210                 215                 220                 


Lys Leu Gly Ile Lys Leu Thr Ile Gly Arg Asp Gly Ser Glu Pro Lys 
225                 230                 235                 240 


Met Gln Arg Ile Gly Asp Met Thr Ala Val Glu Val Lys Gly Arg Ile 
                245                 250                 255     


His Phe Asp Leu Tyr His Val Ile Thr Arg Thr Ile Asn Leu Pro Thr 
            260                 265                 270         


Tyr Thr Leu Glu Ala Val Tyr Glu Ala Ile Phe Gly Lys Pro Lys Glu 
        275                 280                 285             


Lys Val Tyr Ala Asp Glu Ile Ala Lys Ala Trp Glu Ser Gly Glu Asn 
    290                 295                 300                 


Leu Glu Arg Val Ala Lys Tyr Ser Met Glu Asp Ala Lys Ala Thr Tyr 
305                 310                 315                 320 


Glu Leu Gly Lys Glu Phe Leu Pro Met Glu Ile Gln Leu Ser Arg Leu 
                325                 330                 335     


Val Gly Gln Pro Leu Trp Asp Val Ser Arg Ser Ser Thr Gly Asn Leu 
            340                 345                 350         


Val Glu Trp Phe Leu Leu Arg Lys Ala Tyr Glu Arg Asn Glu Val Ala 
        355                 360                 365             


Pro Asn Lys Pro Ser Glu Glu Glu Tyr Gln Arg Arg Leu Arg Glu Ser 
    370                 375                 380                 


Tyr Thr Gly Gly Phe Val Lys Glu Pro Glu Lys Gly Leu Trp Glu Asn 
385                 390                 395                 400 


Ile Val Tyr Leu Asp Phe Arg Ala Leu Gly Pro Ser Ile Ile Ile Thr 
                405                 410                 415     


His Asn Val Ser Pro Asp Thr Leu Asn Leu Glu Gly Cys Lys Asn Tyr 
            420                 425                 430         


Asp Ile Ala Pro Gln Val Gly His Lys Phe Cys Lys Asp Ile Pro Gly 
        435                 440                 445             


Phe Ile Pro Ser Leu Leu Gly His Leu Leu Glu Glu Arg Gln Lys Ile 
    450                 455                 460                 


Lys Thr Lys Met Lys Glu Thr Gln Asp Pro Ile Glu Lys Ile Leu Leu 
465                 470                 475                 480 


Asp Tyr Arg Gln Lys Leu Ile Lys Leu Leu Ala Asn Ser Phe Tyr Gly 
                485                 490                 495     


Tyr Tyr Gly Tyr Ala Lys Ala Arg Trp Tyr Cys Lys Glu Cys Ala Glu 
            500                 505                 510         


Ser Val Thr Ala Trp Gly Arg Lys Tyr Ile Glu Leu Val Trp Lys Glu 
        515                 520                 525             


Leu Glu Glu Lys Phe Gly Phe Lys Val Leu Tyr Ile Asp Thr Asp Gly 
    530                 535                 540                 


Leu Tyr Ala Thr Ile Pro Gly Gly Glu Ser Glu Glu Ile Lys Lys Lys 
545                 550                 555                 560 


Ala Leu Glu Phe Val Lys Tyr Ile Asn Ser Lys Leu Pro Gly Leu Leu 
                565                 570                 575     


Glu Leu Glu Tyr Glu Gly Phe Tyr Lys Arg Gly Phe Phe Val Thr Lys 
            580                 585                 590         


Lys Arg Tyr Ala Val Ile Asp Glu Glu Gly Lys Val Ile Thr Arg Gly 
        595                 600                 605             


Leu Glu Ile Val Arg Arg Asp Trp Ser Glu Ile Ala Lys Glu Thr Gln 
    610                 615                 620                 


Ala Arg Val Leu Glu Thr Ile Leu Lys His Gly Asp Val Glu Glu Ala 
625                 630                 635                 640 


Val Arg Ile Val Lys Glu Val Ile Gln Lys Leu Ala Asn Tyr Glu Ile 
                645                 650                 655     


Pro Pro Glu Lys Leu Ala Ile Tyr Lys Gln Ile Thr Arg Pro Leu His 
            660                 665                 670         


Glu Tyr Lys Ala Ile Gly Pro His Val Ala Val Ala Lys Lys Leu Ala 
        675                 680                 685             


Ala Lys Gly Val Lys Ile Lys Pro Gly Met Val Ile Gly Tyr Ile Val 
    690                 695                 700                 


Leu Arg Gly Asp Gly Pro Ile Ser Asn Arg Ala Ile Leu Ala Glu Glu 
705                 710                 715                 720 


Tyr Asp Pro Lys Lys His Lys Tyr Asp Ala Glu Tyr Tyr Ile Glu Asn 
                725                 730                 735     


Gln Val Leu Pro Ala Val Leu Arg Ile Leu Glu Gly Phe Gly Tyr Arg 
            740                 745                 750         


Lys Glu Asp Leu Arg Tyr Gln Lys Thr Arg Gln Val Gly Leu Thr Ser 
        755                 760                 765             


Trp Leu Asn Ile Lys Lys Ser 
    770                 775 


<210>  77

<400>  77
000

<210>  78

<400>  78
000

<210>  79

<400>  79
000

<210>  80

<400>  80
000

<210>  81

<400>  81
000

<210>  82
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-WT amino acid sequence

<400>  82

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
            180                 185                 190         


Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
        195                 200                 205             


Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
    210                 215                 220                 


Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
225                 230                 235                 240 


Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
                245                 250                 255     


Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
            260                 265                 270         


Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala 
        275                 280                 285             


Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
    290                 295                 300                 


Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile 
305                 310                 315                 320 


Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala 
                325                 330                 335     


Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
            340                 345                 350         


Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp 
        355                 360                 365             


Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
    370                 375                 380                 


Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
385                 390                 395                 400 


Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
                405                 410                 415     


Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
            420                 425                 430         


Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
        435                 440                 445             


Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
    450                 455                 460                 


Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
465                 470                 475                 480 


Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
                485                 490                 495     


Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
            500                 505                 510         


Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
        515                 520                 525             


Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
    530                 535                 540                 


His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
545                 550                 555                 560 


Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
                565                 570                 575     


Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
            580                 585                 590         


Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
        595                 600                 605             


Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
    610                 615                 620                 


Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
625                 630                 635                 640 


Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
                645                 650                 655     


Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
            660                 665                 670         


Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
        675                 680                 685             


Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
    690                 695                 700                 


Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
705                 710                 715                 720 


Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
                725                 730                 735     


Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
            740                 745                 750         


Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
        755                 760                 765             


Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
    770                 775                 780                 


Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
785                 790                 795                 800 


Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
                805                 810                 815     


Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
            820                 825                 830         


Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
        835                 840                 845             


Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
    850                 855                 860                 


Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
865                 870                 875                 880 


Ala Phe Ala 
            


<210>  83
<211>  883
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-37I (I6V, I14L, I74L, I82V, I109V, I117L, I141V, I219M, I244L,
       I281V, I320V, I322L, I330V, I367L)  amino acid sequence

<400>  83

Met Asn Thr Ile Asn Val Ala Lys Asn Asp Phe Ser Asp Leu Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Val Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Val Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Val Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Leu Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Val Gly Arg Ala 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
            180                 185                 190         


Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
        195                 200                 205             


Ser Met His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
    210                 215                 220                 


Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
225                 230                 235                 240 


Ser Glu Thr Leu Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
                245                 250                 255     


Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
            260                 265                 270         


Val Pro Pro Lys Pro Trp Thr Gly Val Thr Gly Gly Gly Tyr Trp Ala 
        275                 280                 285             


Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
    290                 295                 300                 


Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Val 
305                 310                 315                 320 


Asn Leu Ala Gln Asn Thr Ala Trp Lys Val Asn Lys Lys Val Leu Ala 
                325                 330                 335     


Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
            340                 345                 350         


Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Leu Asp 
        355                 360                 365             


Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
    370                 375                 380                 


Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
385                 390                 395                 400 


Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
                405                 410                 415     


Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
            420                 425                 430         


Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
        435                 440                 445             


Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
    450                 455                 460                 


Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
465                 470                 475                 480 


Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
                485                 490                 495     


Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
            500                 505                 510         


Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
        515                 520                 525             


Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
    530                 535                 540                 


His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
545                 550                 555                 560 


Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
                565                 570                 575     


Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
            580                 585                 590         


Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
        595                 600                 605             


Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
    610                 615                 620                 


Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
625                 630                 635                 640 


Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
                645                 650                 655     


Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
            660                 665                 670         


Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
        675                 680                 685             


Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
    690                 695                 700                 


Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
705                 710                 715                 720 


Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
                725                 730                 735     


Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
            740                 745                 750         


Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
        755                 760                 765             


Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
    770                 775                 780                 


Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
785                 790                 795                 800 


Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
                805                 810                 815     


Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
            820                 825                 830         


Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
        835                 840                 845             


Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
    850                 855                 860                 


Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
865                 870                 875                 880 


Ala Phe Ala 
            


<210>  84
<211>  895
<212>  PRT
<213>  Artificial sequence

<220>
<223>  YenP amino acid sequence

<400>  84

Met Asn Ile Leu Asn His Val Ala Arg Asn Asp Phe Ser Glu Met Glu 
1               5                   10                  15      


Leu Ala Ala Ile Pro Tyr Asn Thr Leu Ser Glu His Tyr Gly Asp Arg 
            20                  25                  30          


Leu Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ala Tyr Glu Leu Gly 
        35                  40                  45              


Glu Lys Arg Phe Leu Lys Met Leu Asp Arg Gln Ala Gln Ala Gly Glu 
    50                  55                  60                  


Leu Ala Asp Asn Ala Ala Ala Lys Pro Leu Val Ala Thr Leu Val Pro 
65                  70                  75                  80  


Arg Met Ala Ser Arg Val Thr Glu Trp Val Asp Glu Ser Phe Phe Val 
                85                  90                  95      


Thr Asp Pro Glu Thr Gly Glu Lys Lys Gly Lys Lys Gly Lys Arg Ser 
            100                 105                 110         


Val Ser Gln Arg Ile Leu Arg Glu Val Lys Pro Glu Val Val Ala Phe 
        115                 120                 125             


Val Thr Ile Lys Cys Val Leu Gly Gly Leu Thr Ser Thr Gly Ser Thr 
    130                 135                 140                 


Val Val Gln Arg Leu Ala Ser Ser Val Gly Lys Ala Leu Glu Glu Glu 
145                 150                 155                 160 


Met Arg Phe Gly Arg Ile Arg Asp Leu Glu Gln Lys His Phe Lys Lys 
                165                 170                 175     


Asn Val Gln Glu Gln Leu Glu Lys Arg Val Gly His Val Tyr Lys Lys 
            180                 185                 190         


Ala Phe Leu Gln Val Val Glu Ala Asp Met Leu Ser Lys Gly Leu Met 
        195                 200                 205             


Gly Gly Glu Ala Trp Ser Ser Trp Thr Ala Glu Asp Thr Ile Gln Val 
    210                 215                 220                 


Gly Val Lys Cys Ile Glu Leu Leu Ile Glu Ser Thr Gly Leu Val Glu 
225                 230                 235                 240 


Leu Glu Arg Glu Gly His Gly Ile Ala Gly Ala Asp Ser Glu Asn Val 
                245                 250                 255     


Lys Leu Ala Asp Glu Tyr Met Asp Ile Ile Thr Lys Arg Ala Gly Ala 
            260                 265                 270         


Leu Ala Gly Ile Ala Pro Met His Gln Pro Cys Val Val Pro Pro Lys 
        275                 280                 285             


Pro Trp Thr Gly Val Ile Gly Gly Gly Tyr Trp Ala Asn Gly Arg Lys 
    290                 295                 300                 


Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala Leu Leu Arg Tyr 
305                 310                 315                 320 


Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Val Asn Ile Ala Gln 
                325                 330                 335     


Asn Thr Pro Trp Lys Ile Asn Lys Lys Val Leu Ala Val Val Asn Gln 
            340                 345                 350         


Val Thr Lys Trp Gln His Cys Pro Val Ala Asp Val Pro Ala Gln Glu 
        355                 360                 365             


Arg Glu Glu Leu Pro Gln Arg Pro Asp Asp Met Asp Thr Asn Pro Glu 
    370                 375                 380                 


Ala Leu Lys Ala Trp Lys Lys Ala Ala Ser Ala Val Tyr Arg Lys Asp 
385                 390                 395                 400 


Lys Ala Arg Val Ser Arg Arg Leu Ser Leu Glu Phe Met Leu Ala Gln 
                405                 410                 415     


Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met 
            420                 425                 430         


Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly 
        435                 440                 445             


Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile 
    450                 455                 460                 


Gly Ala Glu Gly Phe Tyr Trp Leu Lys Ile His Gly Ala Asn Thr Ala 
465                 470                 475                 480 


Gly Val Asp Lys Val Thr Phe Pro Glu Arg Ile Lys Phe Ile Glu Asp 
                485                 490                 495     


Asn His Ala Asn Ile Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr 
            500                 505                 510         


Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe 
        515                 520                 525             


Glu Tyr Ala Gly Val Gln Ser His Gly Leu Gly Tyr Val Cys Ser Leu 
    530                 535                 540                 


Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala 
545                 550                 555                 560 


Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser 
                565                 570                 575     


Glu Glu Val Gln Asp Ile Tyr Gly Ile Val Ala Gln Arg Val Asn Glu 
            580                 585                 590         


Met Leu Leu Glu Ala Thr Val Ser Gly Thr Ala Asp Glu Thr Glu Thr 
        595                 600                 605             


Leu Val Asn Lys Asp Thr Gly Glu Ile Thr Glu Arg Leu Lys Leu Gly 
    610                 615                 620                 


Thr Ala Lys Leu Ala Ala Gln Trp Leu Ala Phe Gly Val Thr Arg Lys 
625                 630                 635                 640 


Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe 
                645                 650                 655     


Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Val Gln Pro Ala Leu Asp 
            660                 665                 670         


Asn Gly Gln Gly Leu Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr 
        675                 680                 685             


Met Ala Lys Leu Ile Trp Glu Ala Val Ser Val Thr Val Val Ala Ala 
    690                 695                 700                 


Val Glu Ala Met Asn Trp Leu Lys Ala Ser Ala Lys Leu Leu Ala Ala 
705                 710                 715                 720 


Glu Val Lys Asp Lys Lys Thr Asp Glu Val Leu Arg Ala Arg Cys Ala 
                725                 730                 735     


Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Arg 
            740                 745                 750         


Lys Pro Val Gln Ser Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg 
        755                 760                 765             


Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Gly Ile Asp Ala His 
    770                 775                 780                 


Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly 
785                 790                 795                 800 


Asn His Leu Arg Met Thr Val Val His Ala His Glu Ala Tyr Gly Ile 
                805                 810                 815     


Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp 
            820                 825                 830         


Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met Val Ser Thr Tyr 
        835                 840                 845             


Glu Glu Asn Asp Val Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln 
    850                 855                 860                 


Leu His Glu Ser Gln Leu Glu Lys Met Pro Ala Leu Pro Ala Lys Gly 
865                 870                 875                 880 


Ser Leu Asn Leu Gln Asp Ile Leu Lys Ser Asp Phe Ala Phe Ala 
                885                 890                 895 


<210>  85
<211>  893
<212>  PRT
<213>  Artificial sequence

<220>
<223>  phiEap amino acid sequence

<400>  85

Met Ser Leu Met Asn Ser Ile Glu Arg Asn Asp Phe Ser Asp Ile Glu 
1               5                   10                  15      


Leu Ala Ala Ile Pro Tyr Asn Ile Leu Ser Glu His Tyr Gly Ala Ser 
            20                  25                  30          


Leu Ala Lys Glu Gln Leu Ala Leu Glu His Glu Ala Tyr Glu Leu Gly 
        35                  40                  45              


Glu Gln Arg Phe Leu Lys Thr Phe Glu Arg Gln Met Asn Asn Gly Glu 
    50                  55                  60                  


Leu Ala Asp Asn Ala Ala Ala Lys Pro Leu Val Leu Thr Leu Leu Pro 
65                  70                  75                  80  


Arg Leu Val Ser Arg Ile Asn Gln Trp Arg His Glu Gln Val Tyr Lys 
                85                  90                  95      


Leu Asn Asp Lys Gly Glu Glu Val Leu Arg Gly Gly Lys Lys Thr Ile 
            100                 105                 110         


Met Phe Asn Leu Thr Ala Thr Leu Thr Ala Glu Ala Val Ala Leu Ala 
        115                 120                 125             


Thr Ile Lys Thr Val Leu Ala Ser Leu Thr Ser Thr Asn Ala Val Thr 
    130                 135                 140                 


Ile Gln Glu Ala Gly Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala 
145                 150                 155                 160 


Arg Phe Gly Arg Ile Arg Asp Leu Glu Glu Lys His Phe Lys Lys Asn 
                165                 170                 175     


Val Lys Glu Gln Leu Glu Lys Arg Val Gly Thr Val Tyr Lys Lys Ala 
            180                 185                 190         


Phe Met Gln Val Ile Glu Ala Asp Met Leu Ser Lys Gly Leu Met Gly 
        195                 200                 205             


Gly Gln Ala Trp Ala Ser Trp Ser Lys Glu Asp Ser Ile His Val Gly 
    210                 215                 220                 


Ile Arg Cys Ile Glu Leu Leu Ile Glu Ala Thr Gly Met Val Glu Val 
225                 230                 235                 240 


Val Arg His Gly Ala Gly Ile Ala Lys Leu Asp Ser Glu Asn Leu Gln 
                245                 250                 255     


Leu Thr Lys Glu Tyr Ala Asp Leu Ile Thr Lys Arg Ala Gly Ala Leu 
            260                 265                 270         


Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro 
        275                 280                 285             


Trp Thr Glu Ile Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro 
    290                 295                 300                 


Leu Ala Leu Val Arg Thr His Gly Lys Lys Ala Leu Met Arg Tyr Gln 
305                 310                 315                 320 


Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Ser 
                325                 330                 335     


Thr Pro Trp Lys Ile Asn Gln Lys Val Leu Lys Val Ala Asn Glu Ile 
            340                 345                 350         


Val Asn Trp Lys Asn Cys Pro Val Glu Asp Val Pro Ala Thr Glu Arg 
        355                 360                 365             


Lys Glu Leu Pro Val Lys Pro Asp Asp Ile Asp Thr Asn Pro Glu Ala 
    370                 375                 380                 


Leu Lys Ala Trp Lys Lys Ala Ala Ser Thr Ile Tyr Arg Asn Asp Lys 
385                 390                 395                 400 


Ala Arg Val Ser Arg Arg Ile Ser Met Glu Phe Met Leu Ala Gln Ala 
                405                 410                 415     


Asn Lys Phe Ala Asn Lys Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp 
            420                 425                 430         


Trp Arg Gly Arg Val Tyr Ala Val Pro Met Phe Asn Pro Gln Gly Asn 
        435                 440                 445             


Asp Leu Thr Lys Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly 
    450                 455                 460                 


Leu Asp Gly Leu Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly 
465                 470                 475                 480 


Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn 
                485                 490                 495     


His Asp Asn Ile Met Gln Ser Ala Ala Asn Pro Ile Glu Phe Asp Trp 
            500                 505                 510         


Trp Ala Lys Gln Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu 
        515                 520                 525             


Tyr Ala Gly Val Glu His His Gly Leu Asn Tyr Asn Cys Ser Leu Pro 
    530                 535                 540                 


Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met 
545                 550                 555                 560 


Leu Arg Asp Glu Ile Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Thr 
                565                 570                 575     


Glu Val Gln Asp Ile Tyr Arg Ile Val Ala Asp Lys Val Asn Glu Ile 
            580                 585                 590         


Leu Lys Val Asp Ala Val Asn Gly Thr Ala Asn Glu Val Glu Leu Ile 
        595                 600                 605             


Thr Asp Lys Thr Thr Gly Glu Ile Lys Glu Arg Leu Lys Val Gly Thr 
    610                 615                 620                 


Ser Val Met Ala Gln Glu Trp Leu Ala Phe Gly Val Thr Arg Lys Val 
625                 630                 635                 640 


Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly 
                645                 650                 655     


Phe Arg Gln Gln Ile Leu Asp Asp Thr Val Gln Pro Ala Ile Asp Asn 
            660                 665                 670         


Gly Tyr Thr Gly Phe Thr Asn Gly Asn Gln Ser Ala Gly Tyr Met Ala 
        675                 680                 685             


Lys Leu Ile Trp Asn Ala Val Ser Val Thr Val Val Ala Ala Val Glu 
    690                 695                 700                 


Ala Met Asn Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val 
705                 710                 715                 720 


Lys Asp Lys Lys Thr Lys Glu Val Leu Arg Ala Arg Cys Ala Val His 
                725                 730                 735     


Trp Val Thr Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Arg Lys Ser 
            740                 745                 750         


Lys Gln Val Arg Leu Asp Leu Ile Phe Leu Gly Ser Ile Arg Leu Gln 
        755                 760                 765             


Pro Thr Val Asn Thr Asn Lys Asp Thr Gly Ile Asp Ala His Lys Gln 
    770                 775                 780                 


Glu Ser Gly Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Asn His 
785                 790                 795                 800 


Leu Arg Ala Thr Val Val His Ala His Glu Lys Tyr Gly Ile Glu Ser 
                805                 810                 815     


Phe Ala Leu Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala 
            820                 825                 830         


Asn Leu Phe Lys Ala Val Arg Glu Thr Met Val Glu Thr Tyr Glu Ser 
        835                 840                 845             


Asn Asp Val Leu Ala Asp Phe Tyr Glu Gln Phe Ala Asp Gln Leu His 
    850                 855                 860                 


Glu Ser Gln Leu Glu Asp Met Pro Ala Leu Pro Lys Ala Gly Asn Leu 
865                 870                 875                 880 


Asn Leu Gln Asp Ile Leu Lys Ser Asp Phe Ala Phe Ala 
                885                 890             


<210>  86
<211>  906
<212>  PRT
<213>  Artificial sequence

<220>
<223>  KpnP amino acid sequence

<400>  86

Met Asn Ala Leu Asn Ile Ala Arg Asn Asp Phe Ser Glu Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Tyr Asn Ile Leu Ser Glu His Tyr Gly Asp Lys Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ala Tyr Glu Leu Gly Glu 
        35                  40                  45              


Gln Arg Phe Leu Lys Met Leu Glu Arg Gln Val Lys Ala Gly Glu Phe 
    50                  55                  60                  


Ala Asp Asn Val Ala Ala Lys Pro Leu Val Leu Thr Leu His Pro Gln 
65                  70                  75                  80  


Leu Thr Lys Arg Ile Asp Asp Trp Lys Glu Glu Gln Ala Asn Ala Arg 
                85                  90                  95      


Gly Lys Lys Pro Arg Ala Tyr Tyr Pro Ile Lys His Gly Val Ala Ser 
            100                 105                 110         


Glu Leu Ala Val Asn Met Gly Ala Glu Val Leu Lys Glu Lys Arg Gly 
        115                 120                 125             


Val Ser Ser Glu Ala Ile Ala Leu Leu Thr Ile Lys Val Val Leu Gly 
    130                 135                 140                 


Thr Leu Thr Asp Ala Ser Lys Ala Thr Ile Gln Gln Val Ser Ser Gln 
145                 150                 155                 160 


Leu Gly Lys Ala Leu Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Glu 
                165                 170                 175     


Gln Glu Ala Ala Tyr Phe Lys Lys Asn Val Ala Asp Gln Leu Asp Lys 
            180                 185                 190         


Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala 
        195                 200                 205             


Asp Met Ile Ser Lys Gly Met Leu Gly Gly Asp Asn Trp Ser Ser Trp 
    210                 215                 220                 


Lys Thr Asp Glu Gln Met His Val Gly Thr Lys Leu Leu Glu Leu Leu 
225                 230                 235                 240 


Ile Glu Gly Thr Gly Leu Val Glu Met Thr Lys Asn Lys Met Ala Asp 
                245                 250                 255     


Gly Ser Asp Asp Val Thr Ser Met Gln Met Val Gln Leu Ala Pro Ala 
            260                 265                 270         


Phe Val Glu Leu Leu Ser Lys Arg Ala Gly Ala Leu Ala Gly Ile Ser 
        275                 280                 285             


Pro Met Tyr Gln Pro Cys Val Val Pro Pro Lys Pro Trp Val Glu Thr 
    290                 295                 300                 


Val Gly Gly Gly Tyr Trp Ser Val Gly Arg Arg Pro Leu Ala Leu Val 
305                 310                 315                 320 


Arg Thr His Ser Lys Lys Ala Leu Arg Arg Tyr Glu Asp Val His Met 
                325                 330                 335     


Pro Glu Val Tyr Lys Ala Val Asn Leu Ala Gln Asn Thr Pro Trp Lys 
            340                 345                 350         


Val Asn Lys Lys Val Leu Ala Val Val Asn Glu Ile Ile Asn Trp Lys 
        355                 360                 365             


His Cys Pro Val Gly Asp Val Pro Ala Ile Glu Arg Glu Glu Leu Pro 
    370                 375                 380                 


Pro Arg Pro Asp Asp Ile Asp Thr Asn Glu Val Ala Arg Lys Ala Trp 
385                 390                 395                 400 


Arg Lys Glu Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Gln Ser 
                405                 410                 415     


Arg Arg Leu Ser Met Glu Phe Met Val Ala Gln Ala Asn Lys Phe Ala 
            420                 425                 430         


Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
        435                 440                 445             


Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
    450                 455                 460                 


Gly Met Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Leu Asp Gly Phe 
465                 470                 475                 480 


Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
                485                 490                 495     


Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn Asp Ala Asn Ile 
            500                 505                 510         


Leu Ala Ser Ala Ala Asp Pro Leu Asn Asn Thr Trp Trp Thr Gln Gln 
        515                 520                 525             


Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
    530                 535                 540                 


Lys His His Gly Leu Asn Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
545                 550                 555                 560 


Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Ser 
                565                 570                 575     


Ile Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Asp Thr Val Gln Asp 
            580                 585                 590         


Ile Tyr Lys Ile Val Ala Asp Lys Val Asn Glu Val Leu His Gln His 
        595                 600                 605             


Val Ile Asn Gly Ser Gln Thr Val Val Glu Gln Ile Ala Asp Lys Glu 
    610                 615                 620                 


Thr Gly Glu Phe Arg Glu Lys Val Thr Leu Gly Glu Ser Val Leu Ala 
625                 630                 635                 640 


Ala Gln Trp Leu Gln Tyr Gly Val Thr Arg Lys Val Thr Lys Arg Ser 
                645                 650                 655     


Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
            660                 665                 670         


Val Leu Glu Asp Thr Ile Gln Pro Ala Ile Asp Asn Gly Glu Gly Leu 
        675                 680                 685             


Met Phe Thr His Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
    690                 695                 700                 


Trp Asp Ala Val Thr Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
705                 710                 715                 720 


Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
                725                 730                 735     


Lys Thr Lys Glu Val Leu Arg Lys Arg Cys Ala Ile His Trp Val Thr 
            740                 745                 750         


Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Arg Lys Gln Asn Gln Ala 
        755                 760                 765             


Arg Leu Lys Leu Val Phe Leu Gly Gln Ala Asn Val Lys Met Thr Tyr 
    770                 775                 780                 


Asn Thr Gly Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
785                 790                 795                 800 


Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Met 
                805                 810                 815     


Thr Val Val His Ala Asn Glu Val Tyr Gly Ile Asp Ser Phe Ala Leu 
            820                 825                 830         


Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Gly Asn Leu Phe 
        835                 840                 845             


Lys Ala Val Arg Glu Thr Met Val Lys Thr Tyr Glu Asp Asn Asp Val 
    850                 855                 860                 


Ile Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
865                 870                 875                 880 


Leu Asp Lys Met Pro Ala Val Pro Ala Lys Gly Asp Leu Asn Leu Arg 
                885                 890                 895     


Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala 
            900                 905     


<210>  87
<211>  369
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-split-N fragment amino acid sequence

<400>  87

His His His His His His Met Asn Thr Ile Asn Val Ala Lys Asn Asp 
1               5                   10                  15      


Phe Ser Asp Leu Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp 
            20                  25                  30          


His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His Glu 
        35                  40                  45              


Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg Gln 
    50                  55                  60                  


Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu Val 
65                  70                  75                  80  


Thr Thr Leu Leu Pro Lys Met Val Ala Arg Ile Asn Asp Trp Phe Glu 
                85                  90                  95      


Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu 
            100                 105                 110         


Gln Glu Val Lys Pro Glu Ala Val Ala Tyr Leu Thr Ile Lys Thr Thr 
        115                 120                 125             


Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val Ala 
    130                 135                 140                 


Ser Ala Val Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile 
145                 150                 155                 160 


Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln Leu 
                165                 170                 175     


Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val Val 
            180                 185                 190         


Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser 
        195                 200                 205             


Ser Trp His Lys Glu Asp Ser Met His Val Gly Val Arg Cys Ile Glu 
    210                 215                 220                 


Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn Ala 
225                 230                 235                 240 


Gly Val Val Gly Gln Asp Ser Glu Thr Leu Glu Leu Ala Pro Glu Tyr 
                245                 250                 255     


Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro 
            260                 265                 270         


Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Val Thr 
        275                 280                 285             


Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val Arg 
    290                 295                 300                 


Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met Pro 
305                 310                 315                 320 


Glu Val Tyr Lys Ala Val Asn Leu Ala Gln Asn Thr Ala Trp Lys Val 
                325                 330                 335     


Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys His 
            340                 345                 350         


Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro Met 
        355                 360                 365             


Lys 
    


<210>  88
<211>  43
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-1 amino acid sequence

<400>  88

His His His His His His Met Asn Thr Ile Asn Val Ala Lys Asn Asp 
1               5                   10                  15      


Phe Ser Asp Leu Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp 
            20                  25                  30          


His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu 
        35                  40              


<210>  89
<211>  32
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-2 amino acid sequence

<400>  89

Cys Leu Glu His Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys 
1               5                   10                  15      


Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala 
            20                  25                  30          


<210>  90
<211>  70
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-3 amino acid sequence

<400>  90

Cys Lys Pro Leu Val Thr Thr Leu Leu Pro Lys Met Val Ala Arg Ile 
1               5                   10                  15      


Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr 
            20                  25                  30          


Ala Phe Gln Phe Leu Gln Glu Val Lys Pro Glu Ala Val Ala Tyr Leu 
        35                  40                  45              


Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr 
    50                  55                  60                  


Val Gln Ala Val Ala Ser 
65                  70  


<210>  91
<211>  76
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-4 amino acid sequence

<400>  91

Cys Val Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg 
1               5                   10                  15      


Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn 
            20                  25                  30          


Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu 
        35                  40                  45              


Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser 
    50                  55                  60                  


Trp His Lys Glu Asp Ser Met His Val Gly Val Arg 
65                  70                  75      


<210>  92
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-5 amino acid sequence

<400>  92

Cys Ile Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg 
1               5                   10                  15      


Gln Asn Ala Gly Val Val Gly Gln Asp Ser Glu Thr Leu Glu Leu Ala 
            20                  25                  30          


Pro Glu Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly 
        35                  40                  


<210>  93
<211>  34
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-6 amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  93

Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys 
1               5                   10                  15      


Pro Trp Thr Gly Val Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg 
            20                  25                  30          


Pro Leu 
        


<210>  94
<211>  69
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-N-7 amino acid sequence

<400>  94

Cys Leu Val Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp 
1               5                   10                  15      


Val Tyr Met Pro Glu Val Tyr Lys Ala Val Asn Leu Ala Gln Asn Thr 
            20                  25                  30          


Ala Trp Lys Val Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr 
        35                  40                  45              


Lys Trp Lys His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu 
    50                  55                  60                  


Glu Leu Pro Met Lys 
65                  


<210>  95
<211>  238
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-split-M fragment amino acid sequence

<400>  95

Pro Glu Asp Leu Asp Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg 
1               5                   10                  15      


Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg 
            20                  25                  30          


Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His 
        35                  40                  45              


Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr 
    50                  55                  60                  


Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu 
65                  70                  75                  80  


Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp 
                85                  90                  95      


Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe 
            100                 105                 110         


Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala 
        115                 120                 125             


Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser 
    130                 135                 140                 


Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His 
145                 150                 155                 160 


His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser 
                165                 170                 175     


Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu Val Gly 
            180                 185                 190         


Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr 
        195                 200                 205             


Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile 
    210                 215                 220                 


Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
225                 230                 235             


<210>  96
<211>  45
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-M-1 amino acis sequence

<400>  96

Pro Glu Asp Leu Asp Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg 
1               5                   10                  15      


Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg 
            20                  25                  30          


Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe 
        35                  40                  45  


<210>  97
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-M-2 amino acis sequence

<400>  97

Cys Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly 
1               5                   10                  15      


Arg Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr 
            20                  25                  30          


Lys Gly Leu Leu Thr Leu 
        35              


<210>  98
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-M-3 amino acis sequence

<400>  98

Cys Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile 
1               5                   10                  15      


His Gly Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg 
            20                  25                  30          


Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile Met 
        35                  40                  


<210>  99
<211>  43
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-M-4 amino acis sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  99

Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser 
1               5                   10                  15      


Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His 
            20                  25                  30          


His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu 
        35                  40              


<210>  100
<211>  22
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-M-5 amino acis sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  100

Phe Asp Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg 
1               5                   10                  15      


Asp Glu Val Gly Gly Arg 
            20          


<210>  101
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-M-6 amino acis sequence

<400>  101

Cys Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile 
1               5                   10                  15      


Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly 
            20                  25                  30          


Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        35                  40                  


<210>  102
<211>  282
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-split-C fragment amino acid sequence

<400>  102

Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
1               5                   10                  15      


Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
            20                  25                  30          


Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
        35                  40                  45              


Val Leu Glu Asp Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly Leu 
    50                  55                  60                  


Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
65                  70                  75                  80  


Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
                85                  90                  95      


Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
            100                 105                 110         


Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
        115                 120                 125             


Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
    130                 135                 140                 


Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
145                 150                 155                 160 


Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
                165                 170                 175     


Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
            180                 185                 190         


Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
        195                 200                 205             


Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
    210                 215                 220                 


Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
225                 230                 235                 240 


Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
                245                 250                 255     


Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
            260                 265                 270         


Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala 
        275                 280         


<210>  103
<211>  72
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-C-1 amino acid sequence

<400>  103

Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
1               5                   10                  15      


Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
            20                  25                  30          


Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
        35                  40                  45              


Val Leu Glu Asp Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly Leu 
    50                  55                  60                  


Met Phe Thr Gln Pro Asn Gln Ala 
65                  70          


<210>  104
<211>  49
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-C-2 amino acid sequence

<400>  104

Cys Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr Val 
1               5                   10                  15      


Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys Leu 
            20                  25                  30          


Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg Lys 
        35                  40                  45              


Arg 
    


<210>  105
<211>  75
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-C-3 amino acid sequence

<400>  105

Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp Gln Glu 
1               5                   10                  15      


Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu Gly Gln 
            20                  25                  30          


Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu Ile Asp 
        35                  40                  45              


Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His Ser Gln 
    50                  55                  60                  


Asp Gly Ser His Leu Arg Lys Thr Val Val Trp 
65                  70                  75  


<210>  106
<211>  44
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-C-4 amino acid sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  N-terminal Trifluoroacetyl thiazolidine-4-caboxylic acid 
       (Tfa-Thz) conjugated

<400>  106

His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe 
1               5                   10                  15      


Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu 
            20                  25                  30          


Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val Leu 
        35                  40                  


<210>  107
<211>  41
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T7-C-5 amino acid sequence

<400>  107

Cys Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln Leu 
1               5                   10                  15      


Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp 
            20                  25                  30          


Ile Leu Glu Ser Asp Phe Ala Phe Ala 
        35                  40      


<210>  108

<400>  108
000

<210>  109
<211>  1539
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Tt 16S DNA template nucleic acid sequence

<400>  109
ctcgatcccg cgaaattaat acgactcact ataggtttgt tggagagttt gatcctggct       60

cagggtgaac gctggcggcg tgcctaagac atgcaagtcg tgcgggccgc ggggttttac      120

tccgtggtca gcggcggacg ggtgagtaac gcgtgggtga cctacccgga agagggggac      180

aacccgggga aactcgggct aatcccccat gtggacccgc cccttggggt gtgtccaaag      240

ggctttgccc gcttccggat gggcccgcgt cccatcagct agttggtggg gtaatggccc      300

accaaggcga cgacgggtag ccggtctgag aggatggccg gccacagggg cactgagaca      360

cgggccccac tcctacggga ggcagcagtt aggaatcttc cgcaatgggc gcaagcctga      420

cggagcgacg ccgcttggag gaagaagccc ttcggggtgt aaactcctga acccgggacg      480

aaacccccga cgaggggact gacggtaccg gggtaatagc gccggccaac tccgtgccag      540

cagccgcggt aatacggagg gcgcgagcgt tacccggatt cactgggcgt aaagggcgtg      600

taggcggcct ggggcgtccc atgtgaaaga ccacggctca accgtggggg agcgtgggat      660

acgctcaggc tagacggtgg gagagggtgg tggaattccc ggagtagcgg tgaaatgcgc      720

agataccggg aggaacgccg atggcgaagg cagccacctg gtccacccgt gacgctgagg      780

cgcgaaagcg tggggagcaa accggattag atacccgggt agtccacgcc ctaaacgatg      840

cgcgctaggt ctctgggtct cctgggggcc gaagctaacg cgttaagcgc gccgcctggg      900

gagtacggcc gcaaggctga aactcaaagg aattgacggg ggcccgcaca agcggtggag      960

catgtggttt aattcgaagc aacgcgaaga accttaccag gccttgacat gctagggaac     1020

ccgggtgaaa gcctggggtg ccccgcgagg ggagccctag cacaggtgct gcatggccgt     1080

cgtcagctcg tgccgtgagg tgttgggtta agtcccgcaa cgagcgcaac ccccgccgtt     1140

agttgccagc ggttcggccg ggcactctaa cgggactgcc cgcgaaagcg ggaggaagga     1200

ggggacgacg tctggtcagc atggccctta cggcctgggc gacacacgtg ctacaatgcc     1260

cactacaaag cgatgccacc cggcaacggg gagctaatcg caaaaaggtg ggcccagttc     1320

ggattggggt ctgcaacccg accccatgaa gccggaatcg ctagtaatcg cggatcagcc     1380

atgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacgcc atgggagcgg     1440

gctctacccg aagtcgccgg gagcctacgg gcaggcgccg agggtagggc ccgtgactgg     1500

ggcgaagtcg taacaaggta gctgtaccgg aaggtgcgg                            1539


<210>  110
<211>  113
<212>  DNA
<213>  Artificial sequence

<220>
<223>  tRNA(Ser) DNA template

<400>  110
tggcggagag agggggattt gaacccccgg tagagttgcc cctactccgg ttttcgagac       60

cggtccgttc agccgctccg gcatctctcc tatagtgagt cgtattagaa ccg             113


<210>  111
<211>  163
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L-guanine sensor DNA template

<400>  111
ctcgatcccg cgaaattaat acgactcact atagacgcga ctgaatgaaa tggtgaagga       60

cgggtccaga taatcgcgtg gatatggcac gcaagtttct accgggcacc gtaaatgtcc      120

gactcttgtt gagtagagtg tgagctccgt aactagtcgc gtc                        163


<210>  112
<211>  203
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA template of L- 38-6 ribozyme

<400>  112
ggactaatac gactcactat tagtcattgc cgcacaaaga caaatctccc ctcagagctt       60

gagaacatct acggatgcag aggagggggc cttcggtgga tcaattgtgc accaccgttc      120

tcaacacgta cccgaacata aaaagacctg acaaaaaggc gatgttagac acgcacaggt      180

gccataccca acacatggct gac                                              203


<210>  113
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L- class ? ligase DNA template

<400>  113
ggactaatac gactcactat tgacaatgac aaaaaatcac tattgttgag aacgttggcg       60

ttaaagccac cgggggctgc ctcccctgca tccgaagatg ttctcaagct ctgagggcag      120

atttgtcttt tcc                                                         133


<210>  114
<211>  182
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L- 38-6 ribozyme

<400>  114
agucauugcc gcacaaagac aaaucucccc ucagagcuug agaacaucua cggaugcaga       60

ggagggggcc uucgguggau caauugugca ccaccguucu caacacguac ccgaacauaa      120

aaagaccuga caaaaaggcg auguuagaca cgcacaggug ccauacccaa cacauggcug      180

ac                                                                     182


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L- 5'-FAM-labelled primer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM labelled

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  FAM conjugated

<400>  115
ggaaaagaca aaucugcccu                                                   20


<210>  116
<211>  112
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  L- class - ligase template

<400>  116
gacaaugaca aaaaaucacu auuguugaga acguuggcgu uaaagccacc gggggcugcc       60

uccccugcau ccgaagaugu ucucaagcuc ugagggcaga uuugucuuuu cc              112


