                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  CHIMERIC RECEPTOR

<130>  P120881PCT

<140>  PCT/GB2021/052017
<141>  2021-08-04

<150>  GB 2012181.0
<151>  2020-08-05

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  502
<212>  PRT
<213>  Homo sapiens TGF-beta receptor type-1 (TBRI)

<400>  1

Met Glu Ala Ala Val Ala Ala Pro Arg Pro Arg Leu Leu Leu Leu Val 
1               5                   10                  15      


Leu Ala Ala Ala Ala Ala Ala Ala Ala Ala Leu Leu Pro Gly Ala Thr 
            20                  25                  30          


Ala Leu Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn Phe Thr Cys 
        35                  40                  45              


Val Thr Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr Thr Asp Lys 
    50                  55                  60                  


Val Ile His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu Ile Pro Arg 
65                  70                  75                  80  


Asp Arg Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly Ser Val Thr 
                85                  90                  95      


Thr Thr Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile Glu Leu Pro 
            100                 105                 110         


Thr Thr Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu Leu Ala Ala 
        115                 120                 125             


Val Ile Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu Met Leu Met 
    130                 135                 140                 


Val Tyr Ile Cys His Asn Arg Thr Val Ile His His Arg Val Pro Asn 
145                 150                 155                 160 


Glu Glu Asp Pro Ser Leu Asp Arg Pro Phe Ile Ser Glu Gly Thr Thr 
                165                 170                 175     


Leu Lys Asp Leu Ile Tyr Asp Met Thr Thr Ser Gly Ser Gly Ser Gly 
            180                 185                 190         


Leu Pro Leu Leu Val Gln Arg Thr Ile Ala Arg Thr Ile Val Leu Gln 
        195                 200                 205             


Glu Ser Ile Gly Lys Gly Arg Phe Gly Glu Val Trp Arg Gly Lys Trp 
    210                 215                 220                 


Arg Gly Glu Glu Val Ala Val Lys Ile Phe Ser Ser Arg Glu Glu Arg 
225                 230                 235                 240 


Ser Trp Phe Arg Glu Ala Glu Ile Tyr Gln Thr Val Met Leu Arg His 
                245                 250                 255     


Glu Asn Ile Leu Gly Phe Ile Ala Ala Asp Asn Lys Asp Asn Gly Thr 
            260                 265                 270         


Trp Thr Gln Leu Trp Leu Val Ser Asp Tyr His Glu His Gly Ser Leu 
        275                 280                 285             


Phe Asp Tyr Leu Asn Arg Tyr Thr Val Thr Val Glu Gly Met Ile Lys 
    290                 295                 300                 


Leu Ala Leu Ser Thr Ala Ser Gly Leu Ala His Leu His Met Glu Ile 
305                 310                 315                 320 


Val Gly Thr Gln Gly Lys Pro Ala Ile Ala His Arg Asp Leu Lys Ser 
                325                 330                 335     


Lys Asn Ile Leu Val Lys Lys Asn Gly Thr Cys Cys Ile Ala Asp Leu 
            340                 345                 350         


Gly Leu Ala Val Arg His Asp Ser Ala Thr Asp Thr Ile Asp Ile Ala 
        355                 360                 365             


Pro Asn His Arg Val Gly Thr Lys Arg Tyr Met Ala Pro Glu Val Leu 
    370                 375                 380                 


Asp Asp Ser Ile Asn Met Lys His Phe Glu Ser Phe Lys Arg Ala Asp 
385                 390                 395                 400 


Ile Tyr Ala Met Gly Leu Val Phe Trp Glu Ile Ala Arg Arg Cys Ser 
                405                 410                 415     


Ile Gly Gly Ile His Glu Asp Tyr Gln Leu Pro Tyr Tyr Asp Leu Val 
            420                 425                 430         


Pro Ser Asp Pro Ser Val Glu Glu Met Arg Lys Val Val Cys Glu Gln 
        435                 440                 445             


Lys Leu Arg Pro Asn Ile Pro Asn Arg Trp Gln Ser Cys Glu Ala Leu 
    450                 455                 460                 


Arg Val Met Ala Lys Ile Met Arg Glu Cys Trp Tyr Ala Asn Gly Ala 
465                 470                 475                 480 


Ala Arg Leu Thr Ala Leu Arg Ile Lys Lys Thr Leu Ser Gln Ser Gln 
                485                 490                 495     


Gln Glu Gly Ile Lys Met 
            500         


<210>  2
<211>  567
<212>  PRT
<213>  Homo sapiens TGF-beta receptor type-2 (TBRII)

<400>  2

Met Gly Arg Gly Leu Leu Arg Gly Leu Trp Pro Leu His Ile Val Leu 
1               5                   10                  15      


Trp Thr Arg Ile Ala Ser Thr Ile Pro Pro His Val Gln Lys Ser Val 
            20                  25                  30          


Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro 
        35                  40                  45              


Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln 
    50                  55                  60                  


Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro 
65                  70                  75                  80  


Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr 
                85                  90                  95      


Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile 
            100                 105                 110         


Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys 
        115                 120                 125             


Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn 
    130                 135                 140                 


Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu 
145                 150                 155                 160 


Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu 
                165                 170                 175     


Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg Val Asn 
            180                 185                 190         


Arg Gln Gln Lys Leu Ser Ser Thr Trp Glu Thr Gly Lys Thr Arg Lys 
        195                 200                 205             


Leu Met Glu Phe Ser Glu His Cys Ala Ile Ile Leu Glu Asp Asp Arg 
    210                 215                 220                 


Ser Asp Ile Ser Ser Thr Cys Ala Asn Asn Ile Asn His Asn Thr Glu 
225                 230                 235                 240 


Leu Leu Pro Ile Glu Leu Asp Thr Leu Val Gly Lys Gly Arg Phe Ala 
                245                 250                 255     


Glu Val Tyr Lys Ala Lys Leu Lys Gln Asn Thr Ser Glu Gln Phe Glu 
            260                 265                 270         


Thr Val Ala Val Lys Ile Phe Pro Tyr Glu Glu Tyr Ala Ser Trp Lys 
        275                 280                 285             


Thr Glu Lys Asp Ile Phe Ser Asp Ile Asn Leu Lys His Glu Asn Ile 
    290                 295                 300                 


Leu Gln Phe Leu Thr Ala Glu Glu Arg Lys Thr Glu Leu Gly Lys Gln 
305                 310                 315                 320 


Tyr Trp Leu Ile Thr Ala Phe His Ala Lys Gly Asn Leu Gln Glu Tyr 
                325                 330                 335     


Leu Thr Arg His Val Ile Ser Trp Glu Asp Leu Arg Lys Leu Gly Ser 
            340                 345                 350         


Ser Leu Ala Arg Gly Ile Ala His Leu His Ser Asp His Thr Pro Cys 
        355                 360                 365             


Gly Arg Pro Lys Met Pro Ile Val His Arg Asp Leu Lys Ser Ser Asn 
    370                 375                 380                 


Ile Leu Val Lys Asn Asp Leu Thr Cys Cys Leu Cys Asp Phe Gly Leu 
385                 390                 395                 400 


Ser Leu Arg Leu Asp Pro Thr Leu Ser Val Asp Asp Leu Ala Asn Ser 
                405                 410                 415     


Gly Gln Val Gly Thr Ala Arg Tyr Met Ala Pro Glu Val Leu Glu Ser 
            420                 425                 430         


Arg Met Asn Leu Glu Asn Val Glu Ser Phe Lys Gln Thr Asp Val Tyr 
        435                 440                 445             


Ser Met Ala Leu Val Leu Trp Glu Met Thr Ser Arg Cys Asn Ala Val 
    450                 455                 460                 


Gly Glu Val Lys Asp Tyr Glu Pro Pro Phe Gly Ser Lys Val Arg Glu 
465                 470                 475                 480 


His Pro Cys Val Glu Ser Met Lys Asp Asn Val Leu Arg Asp Arg Gly 
                485                 490                 495     


Arg Pro Glu Ile Pro Ser Phe Trp Leu Asn His Gln Gly Ile Gln Met 
            500                 505                 510         


Val Cys Glu Thr Leu Thr Glu Cys Trp Asp His Asp Pro Glu Ala Arg 
        515                 520                 525             


Leu Thr Ala Gln Cys Val Ala Glu Arg Phe Ser Glu Leu Glu His Leu 
    530                 535                 540                 


Asp Arg Leu Ser Gly Arg Ser Cys Ser Glu Glu Lys Ile Pro Glu Asp 
545                 550                 555                 560 


Gly Ser Leu Asn Thr Thr Lys 
                565         


<210>  3
<211>  144
<212>  PRT
<213>  Unknown

<220>
<223>  extracellular region of TBRII

<400>  3

Thr Ile Pro Pro His Val Gln Lys Ser Val Asn Asn Asp Met Ile Val 
1               5                   10                  15      


Thr Asp Asn Asn Gly Ala Val Lys Phe Pro Gln Leu Cys Lys Phe Cys 
            20                  25                  30          


Asp Val Arg Phe Ser Thr Cys Asp Asn Gln Lys Ser Cys Met Ser Asn 
        35                  40                  45              


Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro Gln Glu Val Cys Val Ala 
    50                  55                  60                  


Val Trp Arg Lys Asn Asp Glu Asn Ile Thr Leu Glu Thr Val Cys His 
65                  70                  75                  80  


Asp Pro Lys Leu Pro Tyr His Asp Phe Ile Leu Glu Asp Ala Ala Ser 
                85                  90                  95      


Pro Lys Cys Ile Met Lys Glu Lys Lys Lys Pro Gly Glu Thr Phe Phe 
            100                 105                 110         


Met Cys Ser Cys Ser Ser Asp Glu Cys Asn Asp Asn Ile Ile Phe Ser 
        115                 120                 125             


Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu Leu Leu Val Ile Phe Gln 
    130                 135                 140                 


<210>  4
<211>  92
<212>  PRT
<213>  Unknown

<220>
<223>  extracellular region of TRBI

<400>  4

Leu Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn Phe Thr Cys Val 
1               5                   10                  15      


Thr Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr Thr Asp Lys Val 
            20                  25                  30          


Ile His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu Ile Pro Arg Asp 
        35                  40                  45              


Arg Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly Ser Val Thr Thr 
    50                  55                  60                  


Thr Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile Glu Leu Pro Thr 
65                  70                  75                  80  


Thr Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu 
                85                  90          


<210>  5
<211>  117
<212>  PRT
<213>  Unknown

<220>
<223>  aROR1 VHH

<400>  5

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ala Ser Ile Ser 
            20                  25                  30          


Pro Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Arg Ile Ser Ser Leu Gly Arg Thr Ile Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Asp Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Phe Glu Asp Thr Gly Val Tyr Tyr Cys Lys 
                85                  90                  95      


Ala Glu Arg Thr Asp Ala Ala Asp Val Tyr Trp Gly Gln Gly Thr Gln 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  6
<211>  113
<212>  PRT
<213>  Unknown

<220>
<223>  aPSMA VHH

<400>  6

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Asp Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Thr Met His Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Thr Met Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Tyr 
                85                  90                  95      


Thr Tyr Tyr Tyr Arg Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
            100                 105                 110         


Ser 
    


<210>  7
<211>  113
<212>  PRT
<213>  Unknown

<220>
<223>  aEGFR VHH

<400>  7

Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Asp Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Thr Met His Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Thr Met Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr Tyr Cys Tyr 
                85                  90                  95      


Thr Tyr Tyr Tyr Arg Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
            100                 105                 110         


Ser 
    


<210>  8
<211>  16
<212>  PRT
<213>  Unknown

<220>
<223>  Glycine /Serine flexible linker

<400>  8

Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


<210>  9
<211>  22
<212>  PRT
<213>  Unknown

<220>
<223>  TBRII transmembrane domain

<400>  9

Val Thr Gly Ile Ser Leu Leu Pro Pro Leu Gly Val Ala Ile Ser Val 
1               5                   10                  15      


Ile Ile Ile Phe Tyr Cys 
            20          


<210>  10
<211>  20
<212>  PRT
<213>  Unknown

<220>
<223>  TBRI transmembrane domain

<400>  10

Leu Ala Ala Val Ile Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu 
1               5                   10                  15      


Met Leu Met Val 
            20  


<210>  11
<211>  27
<212>  PRT
<213>  Unknown

<220>
<223>  CD28 transmembrane domain

<400>  11

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  12
<211>  18
<212>  PRT
<213>  Unknown

<220>
<223>  signal peptide

<400>  12

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His 
        


<210>  13
<211>  19
<212>  PRT
<213>  Unknown

<220>
<223>  signal peptide

<400>  13

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  14
<211>  112
<212>  PRT
<213>  Unknown

<220>
<223>  CD3zeta endodomain

<400>  14

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  15
<211>  30
<212>  PRT
<213>  Unknown

<220>
<223>  Rigid linker

<400>  15

Arg Lys Lys Arg Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala 
1               5                   10                  15      


Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu 
            20                  25                  30  


<210>  16
<211>  626
<212>  PRT
<213>  Unknown

<220>
<223>  SFGmR.HA8-2A-aEGFR_VHH-9G8_16aaLinker-dnTGFbetaRII-RL-2A-dnTGFbet
       aRI-zeta

<400>  16

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe 
        35                  40                  45              


Ser Ser Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg 
    50                  55                  60                  


Glu Phe Val Val Ala Ile Asn Trp Ser Ser Gly Ser Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                85                  90                  95      


Thr Met Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Ala Gly Tyr Gln Ile Asn Ser Gly Asn Tyr Asn Phe 
        115                 120                 125             


Lys Asp Tyr Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val 
    130                 135                 140                 


Ser Ser Arg Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Thr Ile Pro Pro His Val Gln Lys Ser Val Asn Asn Asp 
                165                 170                 175     


Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro Gln Leu Cys 
            180                 185                 190         


Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln Lys Ser Cys 
        195                 200                 205             


Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro Gln Glu Val 
    210                 215                 220                 


Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr Leu Glu Thr 
225                 230                 235                 240 


Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile Leu Glu Asp 
                245                 250                 255     


Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys Pro Gly Glu 
            260                 265                 270         


Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn Asp Asn Ile 
        275                 280                 285             


Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu Leu Leu Val 
    290                 295                 300                 


Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu Gly Val Ala 
305                 310                 315                 320 


Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg Lys Lys Arg Leu Glu 
                325                 330                 335     


Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
            340                 345                 350         


Glu Ala Ala Ala Lys Ala Leu Glu Arg Ala Glu Gly Arg Gly Ser Leu 
        355                 360                 365             


Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly Trp Ser 
    370                 375                 380                 


Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly Val His Ser Leu 
385                 390                 395                 400 


Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn Phe Thr Cys Val Thr 
                405                 410                 415     


Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr Thr Asp Lys Val Ile 
            420                 425                 430         


His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu Ile Pro Arg Asp Arg 
        435                 440                 445             


Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly Ser Val Thr Thr Thr 
    450                 455                 460                 


Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile Glu Leu Pro Thr Thr 
465                 470                 475                 480 


Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu Leu Ala Ala Val Ile 
                485                 490                 495     


Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu Met Leu Met Val Tyr 
            500                 505                 510         


Ile Cys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
        515                 520                 525             


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
    530                 535                 540                 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
545                 550                 555                 560 


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
                565                 570                 575     


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
            580                 585                 590         


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
        595                 600                 605             


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
    610                 615                 620                 


Pro Arg 
625     


<210>  17
<211>  615
<212>  PRT
<213>  Unknown

<220>
<223>  SFGmR.HA8-2A-aROR1_VHH-9G8_16aaLinker-dnTGFbetaRII-RL-2A-dnTGFbet
       aRI-zeta

<400>  17

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln 
            20                  25                  30          


Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ala 
        35                  40                  45              


Ser Ile Ser Pro Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Gln Arg 
    50                  55                  60                  


Glu Phe Val Ala Arg Ile Ser Ser Leu Gly Arg Thr Ile Tyr Ala Asp 
65                  70                  75                  80  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Asp Lys Asn Thr 
                85                  90                  95      


Leu Tyr Leu Gln Met Asn Ser Leu Lys Phe Glu Asp Thr Gly Val Tyr 
            100                 105                 110         


Tyr Cys Lys Ala Glu Arg Thr Asp Ala Ala Asp Val Tyr Trp Gly Gln 
        115                 120                 125             


Gly Thr Gln Val Thr Val Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Thr Ile Pro Pro His Val Gln Lys 
145                 150                 155                 160 


Ser Val Asn Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys 
                165                 170                 175     


Phe Pro Gln Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp 
            180                 185                 190         


Asn Gln Lys Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu 
        195                 200                 205             


Lys Pro Gln Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn 
    210                 215                 220                 


Ile Thr Leu Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp 
225                 230                 235                 240 


Phe Ile Leu Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys 
                245                 250                 255     


Lys Lys Pro Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu 
            260                 265                 270         


Cys Asn Asp Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro 
        275                 280                 285             


Asp Leu Leu Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro 
    290                 295                 300                 


Pro Leu Gly Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg 
305                 310                 315                 320 


Lys Lys Arg Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
                325                 330                 335     


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu Arg Ala Glu 
            340                 345                 350         


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
        355                 360                 365             


Pro Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr 
    370                 375                 380                 


Gly Val His Ser Leu Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn 
385                 390                 395                 400 


Phe Thr Cys Val Thr Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr 
                405                 410                 415     


Thr Asp Lys Val Ile His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu 
            420                 425                 430         


Ile Pro Arg Asp Arg Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly 
        435                 440                 445             


Ser Val Thr Thr Thr Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile 
    450                 455                 460                 


Glu Leu Pro Thr Thr Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu 
465                 470                 475                 480 


Leu Ala Ala Val Ile Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu 
                485                 490                 495     


Met Leu Met Val Tyr Ile Cys Arg Val Lys Phe Ser Arg Ser Ala Asp 
            500                 505                 510         


Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
        515                 520                 525             


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg 
    530                 535                 540                 


Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly 
545                 550                 555                 560 


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
                565                 570                 575     


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
            580                 585                 590         


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
        595                 600                 605             


Met Gln Ala Leu Pro Pro Arg 
    610                 615 


<210>  18
<211>  612
<212>  PRT
<213>  Unknown

<220>
<223>  SFGmR.HA8-2A-aPSMA_VHH-9G8_16aaLinker-dnTGFbetaRII-RL-2A-dnTGFbet
       aRI-zeta

<400>  18

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Asp Ser Gly Ser Ile Phe 
        35                  40                  45              


Arg Ile Asn Thr Met His Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
    50                  55                  60                  


Glu Leu Val Ala Thr Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp 
65                  70                  75                  80  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Lys Asn Thr 
                85                  90                  95      


Met Tyr Leu Gln Met Asn Ser Leu Lys Ala Glu Asp Thr Ala Val Tyr 
            100                 105                 110         


Tyr Cys Tyr Thr Tyr Tyr Tyr Arg Ala Tyr Trp Gly Gln Gly Thr Gln 
        115                 120                 125             


Val Thr Val Ser Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Thr Ile Pro Pro His Val Gln Lys Ser Val Asn 
145                 150                 155                 160 


Asn Asp Met Ile Val Thr Asp Asn Asn Gly Ala Val Lys Phe Pro Gln 
                165                 170                 175     


Leu Cys Lys Phe Cys Asp Val Arg Phe Ser Thr Cys Asp Asn Gln Lys 
            180                 185                 190         


Ser Cys Met Ser Asn Cys Ser Ile Thr Ser Ile Cys Glu Lys Pro Gln 
        195                 200                 205             


Glu Val Cys Val Ala Val Trp Arg Lys Asn Asp Glu Asn Ile Thr Leu 
    210                 215                 220                 


Glu Thr Val Cys His Asp Pro Lys Leu Pro Tyr His Asp Phe Ile Leu 
225                 230                 235                 240 


Glu Asp Ala Ala Ser Pro Lys Cys Ile Met Lys Glu Lys Lys Lys Pro 
                245                 250                 255     


Gly Glu Thr Phe Phe Met Cys Ser Cys Ser Ser Asp Glu Cys Asn Asp 
            260                 265                 270         


Asn Ile Ile Phe Ser Glu Glu Tyr Asn Thr Ser Asn Pro Asp Leu Leu 
        275                 280                 285             


Leu Val Ile Phe Gln Val Thr Gly Ile Ser Leu Leu Pro Pro Leu Gly 
    290                 295                 300                 


Val Ala Ile Ser Val Ile Ile Ile Phe Tyr Cys Tyr Arg Lys Lys Arg 
305                 310                 315                 320 


Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
                325                 330                 335     


Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu Arg Ala Glu Gly Arg Gly 
            340                 345                 350         


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
        355                 360                 365             


Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly Val His 
    370                 375                 380                 


Ser Leu Gln Cys Phe Cys His Leu Cys Thr Lys Asp Asn Phe Thr Cys 
385                 390                 395                 400 


Val Thr Asp Gly Leu Cys Phe Val Ser Val Thr Glu Thr Thr Asp Lys 
                405                 410                 415     


Val Ile His Asn Ser Met Cys Ile Ala Glu Ile Asp Leu Ile Pro Arg 
            420                 425                 430         


Asp Arg Pro Phe Val Cys Ala Pro Ser Ser Lys Thr Gly Ser Val Thr 
        435                 440                 445             


Thr Thr Tyr Cys Cys Asn Gln Asp His Cys Asn Lys Ile Glu Leu Pro 
    450                 455                 460                 


Thr Thr Val Lys Ser Ser Pro Gly Leu Gly Pro Val Glu Leu Ala Ala 
465                 470                 475                 480 


Val Ile Ala Gly Pro Val Cys Phe Val Cys Ile Ser Leu Met Leu Met 
                485                 490                 495     


Val Tyr Ile Cys Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala 
            500                 505                 510         


Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg 
        515                 520                 525             


Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu 
    530                 535                 540                 


Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
545                 550                 555                 560 


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
                565                 570                 575     


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
            580                 585                 590         


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
        595                 600                 605             


Leu Pro Pro Arg 
    610         


<210>  19
<211>  20
<212>  PRT
<213>  Unknown

<220>
<223>  FMDV 2a self-cleaving peptide

<400>  19

Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


