                         SEQUENCE LISTING

<110>  Nuclera Nucleics Ltd
 
<120>  Modified terminal deoxynucleotidyl transferase (TdT) enzymes

<130>  P33325WO

<160>  4   

<170>  PatentIn version 3.5

<210>  1
<211>  494
<212>  PRT
<213>  Lepisosteus oculatus

<400>  1

Met Leu His Ile Pro Ile Phe Pro Pro Ile Lys Lys Arg Gln Lys Leu 
1               5                   10                  15      


Pro Glu Ser Arg Asn Ser Cys Lys Tyr Glu Val Lys Phe Ser Glu Val 
            20                  25                  30          


Ala Ile Phe Leu Val Glu Arg Lys Met Gly Ser Ser Arg Arg Lys Phe 
        35                  40                  45              


Leu Thr Asn Leu Ala Arg Ser Lys Gly Phe Arg Ile Glu Asp Val Leu 
    50                  55                  60                  


Ser Asp Ala Val Thr His Val Val Ala Glu Asp Asn Ser Ala Asp Glu 
65                  70                  75                  80  


Leu Trp Gln Trp Leu Gln Asn Ser Ser Leu Gly Asp Leu Ser Lys Ile 
                85                  90                  95      


Glu Val Leu Asp Ile Ser Trp Phe Thr Glu Cys Met Gly Ala Gly Lys 
            100                 105                 110         


Pro Val Gln Val Glu Ala Arg His Cys Leu Val Lys Ser Cys Pro Val 
        115                 120                 125             


Ile Asp Gln Tyr Leu Glu Pro Ser Thr Val Glu Thr Val Ser Gln Tyr 
    130                 135                 140                 


Ala Cys Gln Arg Arg Thr Thr Met Glu Asn His Asn Gln Ile Phe Thr 
145                 150                 155                 160 


Asp Ala Phe Ala Ile Leu Ala Glu Asn Ala Glu Phe Asn Glu Ser Glu 
                165                 170                 175     


Gly Pro Cys Leu Ala Phe Met Arg Ala Ala Ser Leu Leu Lys Ser Leu 
            180                 185                 190         


Pro His Ala Ile Ser Ser Ser Lys Asp Leu Glu Gly Leu Pro Cys Leu 
        195                 200                 205             


Gly Asp Gln Thr Lys Ala Val Ile Glu Asp Ile Leu Glu Tyr Gly Gln 
    210                 215                 220                 


Cys Ser Lys Val Gln Asp Val Leu Cys Asp Asp Arg Tyr Gln Thr Ile 
225                 230                 235                 240 


Lys Leu Phe Thr Ser Val Phe Gly Val Gly Leu Lys Thr Ala Glu Lys 
                245                 250                 255     


Trp Tyr Arg Lys Gly Phe His Ser Leu Glu Glu Val Gln Ala Asp Asn 
            260                 265                 270         


Ala Ile His Phe Thr Lys Met Gln Lys Ala Gly Phe Leu Tyr Tyr Asp 
        275                 280                 285             


Asp Ile Ser Ala Ala Val Cys Lys Ala Glu Ala Gln Ala Ile Gly Gln 
    290                 295                 300                 


Ile Val Glu Glu Thr Val Arg Leu Ile Ala Pro Asp Ala Ile Val Thr 
305                 310                 315                 320 


Leu Thr Gly Gly Phe Arg Arg Gly Lys Glu Cys Gly His Asp Val Asp 
                325                 330                 335     


Phe Leu Ile Thr Thr Pro Glu Met Gly Lys Glu Val Trp Leu Leu Asn 
            340                 345                 350         


Arg Leu Ile Asn Arg Leu Gln Asn Gln Gly Ile Leu Leu Tyr Tyr Asp 
        355                 360                 365             


Ile Val Glu Ser Thr Phe Asp Lys Thr Arg Leu Pro Cys Arg Lys Phe 
    370                 375                 380                 


Glu Ala Met Asp His Phe Gln Lys Cys Phe Ala Ile Ile Lys Leu Lys 
385                 390                 395                 400 


Lys Glu Leu Ala Ala Gly Arg Val Gln Lys Asp Trp Lys Ala Ile Arg 
                405                 410                 415     


Val Asp Phe Val Ala Pro Pro Val Asp Asn Phe Ala Phe Ala Leu Leu 
            420                 425                 430         


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Phe Ala 
        435                 440                 445             


Arg His Glu Arg Lys Met Leu Leu Asp Asn His Ala Leu Tyr Asp Lys 
    450                 455                 460                 


Thr Lys Lys Ile Phe Leu Pro Ala Lys Thr Glu Glu Asp Ile Phe Ala 
465                 470                 475                 480 


His Leu Gly Leu Asp Tyr Ile Asp Pro Trp Gln Arg Asn Ala 
                485                 490                 


<210>  2
<211>  509
<212>  PRT
<213>  Bos taurus

<400>  2

Met Asp Pro Leu Cys Thr Ala Ser Ser Gly Pro Arg Lys Lys Arg Pro 
1               5                   10                  15      


Arg Gln Val Gly Ala Ser Met Ala Ser Pro Pro His Asp Ile Lys Phe 
            20                  25                  30          


Gln Asn Leu Val Leu Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg 
        35                  40                  45              


Arg Asn Phe Leu Met Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Glu Val Leu Glu Trp Leu Gln Val Gln Asn Ile Arg Ala Ser 
                85                  90                  95      


Ser Gln Leu Glu Leu Leu Asp Val Ser Trp Leu Ile Glu Ser Met Gly 
            100                 105                 110         


Ala Gly Lys Pro Val Glu Ile Thr Gly Lys His Gln Leu Val Val Arg 
        115                 120                 125             


Thr Asp Tyr Ser Ala Thr Pro Asn Pro Gly Phe Gln Lys Thr Pro Pro 
    130                 135                 140                 


Leu Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Lys Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Tyr Asn His Ile Phe Thr Asp Ala Phe Glu Ile Leu Ala 
                165                 170                 175     


Glu Asn Ser Glu Phe Lys Glu Asn Glu Val Ser Tyr Val Thr Phe Met 
            180                 185                 190         


Arg Ala Ala Ser Val Leu Lys Ser Leu Pro Phe Thr Ile Ile Ser Met 
        195                 200                 205             


Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Cys Ile 
    210                 215                 220                 


Ile Glu Glu Ile Ile Glu Asp Gly Glu Ser Ser Glu Val Lys Ala Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Gln Ser Phe Lys Leu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Leu Lys Thr Ser Glu Lys Trp Phe Arg Met Gly Phe Arg 
            260                 265                 270         


Ser Leu Ser Lys Ile Met Ser Asp Lys Thr Leu Lys Phe Thr Lys Met 
        275                 280                 285             


Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Thr 
    290                 295                 300                 


Arg Ala Glu Ala Glu Ala Val Gly Val Leu Val Lys Glu Ala Val Trp 
305                 310                 315                 320 


Ala Phe Leu Pro Asp Ala Phe Val Thr Met Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Lys Ile Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro Gly 
            340                 345                 350         


Ser Ala Glu Asp Glu Glu Gln Leu Leu Pro Lys Val Ile Asn Leu Trp 
        355                 360                 365             


Glu Lys Lys Gly Leu Leu Leu Tyr Tyr Asp Leu Val Glu Ser Thr Phe 
    370                 375                 380                 


Glu Lys Phe Lys Leu Pro Ser Arg Gln Val Asp Thr Leu Asp His Phe 
385                 390                 395                 400 


Gln Lys Cys Phe Leu Ile Leu Lys Leu His His Gln Arg Val Asp Ser 
                405                 410                 415     


Ser Lys Ser Asn Gln Gln Glu Gly Lys Thr Trp Lys Ala Ile Arg Val 
            420                 425                 430         


Asp Leu Val Met Cys Pro Tyr Glu Asn Arg Ala Phe Ala Leu Leu Gly 
        435                 440                 445             


Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Ile Arg Arg Tyr Ala Thr 
    450                 455                 460                 


His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Lys Thr 
465                 470                 475                 480 


Lys Arg Val Phe Leu Lys Ala Glu Ser Glu Glu Glu Ile Phe Ala His 
                485                 490                 495     


Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg Asn Ala 
            500                 505                 


<210>  3
<211>  530
<212>  PRT
<213>  Mus musculus

<400>  3

Met Asp Pro Leu Gln Ala Val His Leu Gly Pro Arg Lys Lys Arg Pro 
1               5                   10                  15      


Arg Gln Leu Gly Thr Pro Val Ala Ser Thr Pro Tyr Asp Ile Arg Phe 
            20                  25                  30          


Arg Asp Leu Val Leu Phe Ile Leu Glu Lys Lys Met Gly Thr Thr Arg 
        35                  40                  45              


Arg Ala Phe Leu Met Glu Leu Ala Arg Arg Lys Gly Phe Arg Val Glu 
    50                  55                  60                  


Asn Glu Leu Ser Asp Ser Val Thr His Ile Val Ala Glu Asn Asn Ser 
65                  70                  75                  80  


Gly Ser Asp Val Leu Glu Trp Leu Gln Leu Gln Asn Ile Lys Ala Ser 
                85                  90                  95      


Ser Glu Leu Glu Leu Leu Asp Ile Ser Trp Leu Ile Glu Cys Met Gly 
            100                 105                 110         


Ala Gly Lys Pro Val Glu Met Met Gly Arg His Gln Leu Val Val Asn 
        115                 120                 125             


Arg Asn Ser Ser Pro Ser Pro Val Pro Gly Ser Gln Asn Val Pro Ala 
    130                 135                 140                 


Pro Ala Val Lys Lys Ile Ser Gln Tyr Ala Cys Gln Arg Arg Thr Thr 
145                 150                 155                 160 


Leu Asn Asn Tyr Asn Gln Leu Phe Thr Asp Ala Leu Asp Ile Leu Ala 
                165                 170                 175     


Glu Asn Asp Glu Leu Arg Glu Asn Glu Gly Ser Cys Leu Ala Phe Met 
            180                 185                 190         


Arg Ala Ser Ser Val Leu Lys Ser Leu Pro Phe Pro Ile Thr Ser Met 
        195                 200                 205             


Lys Asp Thr Glu Gly Ile Pro Cys Leu Gly Asp Lys Val Lys Ser Ile 
    210                 215                 220                 


Ile Glu Gly Ile Ile Glu Asp Gly Glu Ser Ser Glu Ala Lys Ala Val 
225                 230                 235                 240 


Leu Asn Asp Glu Arg Tyr Lys Ser Phe Lys Leu Phe Thr Ser Val Phe 
                245                 250                 255     


Gly Val Gly Leu Lys Thr Ala Glu Lys Trp Phe Arg Met Gly Phe Arg 
            260                 265                 270         


Thr Leu Ser Lys Ile Gln Ser Asp Lys Ser Leu Arg Phe Thr Gln Met 
        275                 280                 285             


Gln Lys Ala Gly Phe Leu Tyr Tyr Glu Asp Leu Val Ser Cys Val Asn 
    290                 295                 300                 


Arg Pro Glu Ala Glu Ala Val Ser Met Leu Val Lys Glu Ala Val Val 
305                 310                 315                 320 


Thr Phe Leu Pro Asp Ala Leu Val Thr Met Thr Gly Gly Phe Arg Arg 
                325                 330                 335     


Gly Lys Met Thr Gly His Asp Val Asp Phe Leu Ile Thr Ser Pro Glu 
            340                 345                 350         


Ala Thr Glu Asp Glu Glu Gln Gln Leu Leu His Lys Val Thr Asp Phe 
        355                 360                 365             


Trp Lys Gln Gln Gly Leu Leu Leu Tyr Cys Asp Ile Leu Glu Ser Thr 
    370                 375                 380                 


Phe Glu Lys Phe Lys Gln Pro Ser Arg Lys Val Asp Ala Leu Asp His 
385                 390                 395                 400 


Phe Gln Lys Cys Phe Leu Ile Leu Lys Leu Asp His Gly Arg Val His 
                405                 410                 415     


Ser Glu Lys Ser Gly Gln Gln Glu Gly Lys Gly Trp Lys Ala Ile Arg 
            420                 425                 430         


Val Asp Leu Val Met Cys Pro Tyr Asp Arg Arg Ala Phe Ala Leu Leu 
        435                 440                 445             


Gly Trp Thr Gly Ser Arg Gln Phe Glu Arg Asp Leu Arg Arg Tyr Ala 
    450                 455                 460                 


Thr His Glu Arg Lys Met Met Leu Asp Asn His Ala Leu Tyr Asp Arg 
465                 470                 475                 480 


Thr Lys Gly Lys Thr Val Thr Ile Ser Pro Leu Asp Gly Lys Val Ser 
                485                 490                 495     


Lys Leu Gln Lys Ala Leu Arg Val Phe Leu Glu Ala Glu Ser Glu Glu 
            500                 505                 510         


Glu Ile Phe Ala His Leu Gly Leu Asp Tyr Ile Glu Pro Trp Glu Arg 
        515                 520                 525             


Asn Ala 
    530 


<210>  4
<211>  494
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Leu Pro Lys Arg Arg Arg Ala Arg Val Gly Ser Pro Ser Gly Asp 
1               5                   10                  15      


Ala Ala Ser Ser Thr Pro Pro Ser Thr Arg Phe Pro Gly Val Ala Ile 
            20                  25                  30          


Tyr Leu Val Glu Pro Arg Met Gly Arg Ser Arg Arg Ala Phe Leu Thr 
        35                  40                  45              


Gly Leu Ala Arg Ser Lys Gly Phe Arg Val Leu Asp Ala Cys Ser Ser 
    50                  55                  60                  


Glu Ala Thr His Val Val Met Glu Glu Thr Ser Ala Glu Glu Ala Val 
65                  70                  75                  80  


Ser Trp Gln Glu Arg Arg Met Ala Ala Ala Pro Pro Gly Cys Thr Pro 
                85                  90                  95      


Pro Ala Leu Leu Asp Ile Ser Trp Leu Thr Glu Ser Leu Gly Ala Gly 
            100                 105                 110         


Gln Pro Val Pro Val Glu Cys Arg His Arg Leu Glu Val Ala Gly Pro 
        115                 120                 125             


Arg Lys Gly Pro Leu Ser Pro Ala Trp Met Pro Ala Tyr Ala Cys Gln 
    130                 135                 140                 


Arg Pro Thr Pro Leu Thr His His Asn Thr Gly Leu Ser Glu Ala Leu 
145                 150                 155                 160 


Glu Ile Leu Ala Glu Ala Ala Gly Phe Glu Gly Ser Glu Gly Arg Leu 
                165                 170                 175     


Leu Thr Phe Cys Arg Ala Ala Ser Val Leu Lys Ala Leu Pro Ser Pro 
            180                 185                 190         


Val Thr Thr Leu Ser Gln Leu Gln Gly Leu Pro His Phe Gly Glu His 
        195                 200                 205             


Ser Ser Arg Val Val Gln Glu Leu Leu Glu His Gly Val Cys Glu Glu 
    210                 215                 220                 


Val Glu Arg Val Arg Arg Ser Glu Arg Tyr Gln Thr Met Lys Leu Phe 
225                 230                 235                 240 


Thr Gln Ile Phe Gly Val Gly Val Lys Thr Ala Asp Arg Trp Tyr Arg 
                245                 250                 255     


Glu Gly Leu Arg Thr Leu Asp Asp Leu Arg Glu Gln Pro Gln Lys Leu 
            260                 265                 270         


Thr Gln Gln Gln Lys Ala Gly Leu Gln His His Gln Asp Leu Ser Thr 
        275                 280                 285             


Pro Val Leu Arg Ser Asp Val Asp Ala Leu Gln Gln Val Val Glu Glu 
    290                 295                 300                 


Ala Val Gly Gln Ala Leu Pro Gly Ala Thr Val Thr Leu Thr Gly Gly 
305                 310                 315                 320 


Phe Arg Arg Gly Lys Leu Gln Gly His Asp Val Asp Phe Leu Ile Thr 
                325                 330                 335     


His Pro Lys Glu Gly Gln Glu Ala Gly Leu Leu Pro Arg Val Met Cys 
            340                 345                 350         


Arg Leu Gln Asp Gln Gly Leu Ile Leu Tyr His Gln His Gln His Ser 
        355                 360                 365             


Cys Cys Glu Ser Pro Thr Arg Leu Ala Gln Gln Ser His Met Asp Ala 
    370                 375                 380                 


Phe Glu Arg Ser Phe Cys Ile Phe Arg Leu Pro Gln Pro Pro Gly Ala 
385                 390                 395                 400 


Ala Val Gly Gly Ser Thr Arg Pro Cys Pro Ser Trp Lys Ala Val Arg 
                405                 410                 415     


Val Asp Leu Val Val Ala Pro Val Ser Gln Phe Pro Phe Ala Leu Leu 
            420                 425                 430         


Gly Trp Thr Gly Ser Lys Leu Phe Gln Arg Glu Leu Arg Arg Phe Ser 
        435                 440                 445             


Arg Lys Glu Lys Gly Leu Trp Leu Asn Ser His Gly Leu Phe Asp Pro 
    450                 455                 460                 


Glu Gln Lys Thr Phe Phe Gln Ala Ala Ser Glu Glu Asp Ile Phe Arg 
465                 470                 475                 480 


His Leu Gly Leu Glu Tyr Leu Pro Pro Glu Gln Arg Asn Ala 
                485                 490                 



