                         SEQUENCE LISTING

<110>  ǧҽҩ޹˾
 
<120>  һֻ˾׵͹״S׶

<130>  

<160>  11    

<170>  PatentIn version 3.3

<210>  1
<211>  223
<212>  PRT
<213>  RBDİ

<400>  1

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  2
<211>  13
<212>  PRT
<213>  STİ

<400>  2

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  3
<211>  239
<212>  PRT
<213>  ST-RBDİ

<400>  3

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser Gly 
1               5                   10                  15      


Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
            20                  25                  30          


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
        35                  40                  45              


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
    50                  55                  60                  


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
65                  70                  75                  80  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
                85                  90                  95      


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
            100                 105                 110         


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
        115                 120                 125             


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
    130                 135                 140                 


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
145                 150                 155                 160 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
                165                 170                 175     


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
            180                 185                 190         


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
        195                 200                 205             


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
    210                 215                 220                 


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
225                 230                 235                 


<210>  4
<211>  304
<212>  PRT
<213>  HRİ

<400>  4

Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
1               5                   10                  15      


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
            20                  25                  30          


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
        35                  40                  45              


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
    50                  55                  60                  


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
65                  70                  75                  80  


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
                85                  90                  95      


Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser 
            100                 105                 110         


Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser 
        115                 120                 125             


Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro 
    130                 135                 140                 


Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro 
145                 150                 155                 160 


Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly 
                165                 170                 175     


Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His 
            180                 185                 190         


Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr 
        195                 200                 205             


Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val 
    210                 215                 220                 


Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys 
225                 230                 235                 240 


Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp 
                245                 250                 255     


Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys 
            260                 265                 270         


Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu 
        275                 280                 285             


Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro 
    290                 295                 300                 


<210>  5
<211>  320
<212>  PRT
<213>  ST-HRİ

<400>  5

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser Gly 
1               5                   10                  15      


Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn 
            20                  25                  30          


Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr 
        35                  40                  45              


Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln 
    50                  55                  60                  


Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile 
65                  70                  75                  80  


Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala 
                85                  90                  95      


Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln 
            100                 105                 110         


Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser 
        115                 120                 125             


Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser 
    130                 135                 140                 


Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro 
145                 150                 155                 160 


Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro 
                165                 170                 175     


Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly 
            180                 185                 190         


Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His 
        195                 200                 205             


Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr 
    210                 215                 220                 


Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val 
225                 230                 235                 240 


Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys 
                245                 250                 255     


Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp 
            260                 265                 270         


Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys 
        275                 280                 285             


Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu 
    290                 295                 300                 


Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro 
305                 310                 315                 320 


<210>  6
<211>  163
<212>  PRT
<213>  HPFİ

<400>  6

Asp Ile Ile Lys Leu Leu Asn Glu Gln Val Asn Lys Glu Met Gln Ser 
1               5                   10                  15      


Ser Asn Leu Tyr Met Ser Met Ser Ser Trp Cys Tyr Thr His Ser Leu 
            20                  25                  30          


Asp Gly Ala Gly Leu Phe Leu Phe Asp His Ala Ala Glu Glu Tyr Glu 
        35                  40                  45              


His Ala Lys Lys Leu Ile Ile Phe Leu Asn Glu Asn Asn Val Pro Val 
    50                  55                  60                  


Gln Leu Thr Ser Ile Ser Ala Pro Glu His Lys Phe Glu Gly Leu Thr 
65                  70                  75                  80  


Gln Ile Phe Gln Lys Ala Tyr Glu His Glu Gln His Ile Ser Glu Ser 
                85                  90                  95      


Ile Asn Asn Ile Val Asp His Ala Ile Lys Ser Lys Asp His Ala Thr 
            100                 105                 110         


Phe Asn Phe Leu Gln Trp Tyr Val Ala Glu Gln His Glu Glu Glu Val 
        115                 120                 125             


Leu Phe Lys Asp Ile Leu Asp Lys Ile Glu Leu Ile Gly Asn Glu Asn 
    130                 135                 140                 


His Gly Leu Tyr Leu Ala Asp Gln Tyr Val Lys Gly Ile Ala Lys Ser 
145                 150                 155                 160 


Arg Lys Ser 
            


<210>  7
<211>  106
<212>  PRT
<213>  SCİ

<400>  7

Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Asp Ser 
1               5                   10                  15      


Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu 
            20                  25                  30          


Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser 
        35                  40                  45              


Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly 
    50                  55                  60                  


Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala 
65                  70                  75                  80  


Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn 
                85                  90                  95      


Gly Lys Ala Thr Lys Gly Asp Ala His Ile 
            100                 105     


<210>  8
<211>  278
<212>  PRT
<213>  ںϵSC-HPFİУSP-His-tag

<400>  8

Asp Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Asp Ser 
1               5                   10                  15      


Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu 
            20                  25                  30          


Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser 
        35                  40                  45              


Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly 
    50                  55                  60                  


Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala 
65                  70                  75                  80  


Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn 
                85                  90                  95      


Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly Ser Gly Asp Ile Ile 
            100                 105                 110         


Lys Leu Leu Asn Glu Gln Val Asn Lys Glu Met Gln Ser Ser Asn Leu 
        115                 120                 125             


Tyr Met Ser Met Ser Ser Trp Cys Tyr Thr His Ser Leu Asp Gly Ala 
    130                 135                 140                 


Gly Leu Phe Leu Phe Asp His Ala Ala Glu Glu Tyr Glu His Ala Lys 
145                 150                 155                 160 


Lys Leu Ile Ile Phe Leu Asn Glu Asn Asn Val Pro Val Gln Leu Thr 
                165                 170                 175     


Ser Ile Ser Ala Pro Glu His Lys Phe Glu Gly Leu Thr Gln Ile Phe 
            180                 185                 190         


Gln Lys Ala Tyr Glu His Glu Gln His Ile Ser Glu Ser Ile Asn Asn 
        195                 200                 205             


Ile Val Asp His Ala Ile Lys Ser Lys Asp His Ala Thr Phe Asn Phe 
    210                 215                 220                 


Leu Gln Trp Tyr Val Ala Glu Gln His Glu Glu Glu Val Leu Phe Lys 
225                 230                 235                 240 


Asp Ile Leu Asp Lys Ile Glu Leu Ile Gly Asn Glu Asn His Gly Leu 
                245                 250                 255     


Tyr Leu Ala Asp Gln Tyr Val Lys Gly Ile Ala Lys Ser Arg Lys Ser 
            260                 265                 270         


His His His His His His 
        275             


<210>  9
<211>  276
<212>  PRT
<213>  SP-His-tag-ST-RBDİ

<400>  9

Met Gly Ile Leu Pro Ser Pro Gly Met Pro Ala Leu Leu Ser Leu Val 
1               5                   10                  15      


Ser Leu Leu Ser Val Leu Leu Met Gly Cys Val Ala Gly Ser Gly Ala 
            20                  25                  30          


His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser Gly Arg 
        35                  40                  45              


Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
    50                  55                  60                  


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
65                  70                  75                  80  


Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
                85                  90                  95      


Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
            100                 105                 110         


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
        115                 120                 125             


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
    130                 135                 140                 


Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
145                 150                 155                 160 


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
                165                 170                 175     


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
            180                 185                 190         


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
        195                 200                 205             


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
    210                 215                 220                 


Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
225                 230                 235                 240 


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
                245                 250                 255     


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe His His 
            260                 265                 270         


His His His His 
        275     


<210>  10
<211>  357
<212>  PRT
<213>  SP-His-tag-ST-HRİ

<400>  10

Met Gly Ile Leu Pro Ser Pro Gly Met Pro Ala Leu Leu Ser Leu Val 
1               5                   10                  15      


Ser Leu Leu Ser Val Leu Leu Met Gly Cys Val Ala Gly Ser Gly Ala 
            20                  25                  30          


His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys Gly Ser Gly Gly 
        35                  40                  45              


Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln 
    50                  55                  60                  


Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala 
65                  70                  75                  80  


Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala 
                85                  90                  95      


Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser 
            100                 105                 110         


Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu 
        115                 120                 125             


Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr 
    130                 135                 140                 


Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala 
145                 150                 155                 160 


Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys 
                165                 170                 175     


Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln 
            180                 185                 190         


Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala 
        195                 200                 205             


Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys 
    210                 215                 220                 


Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp 
225                 230                 235                 240 


Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp 
                245                 250                 255     


Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn 
            260                 265                 270         


Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu 
        275                 280                 285             


Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu 
    290                 295                 300                 


Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu 
305                 310                 315                 320 


Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile 
                325                 330                 335     


Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro His 
            340                 345                 350         


His His His His His 
        355         


<210>  11
<211>  309
<212>  PRT
<213>  SP-His-tag-SC-HPFİ

<400>  11

Met Gly Ile Leu Pro Ser Pro Gly Met Pro Ala Leu Leu Ser Leu Val 
1               5                   10                  15      


Ser Leu Leu Ser Val Leu Leu Met Gly Cys Val Ala Gly Ser Gly Asp 
            20                  25                  30          


Tyr Asp Ile Pro Thr Thr Glu Asn Leu Tyr Phe Gln Gly Asp Ser Ala 
        35                  40                  45              


Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys Glu Leu Ala 
    50                  55                  60                  


Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr Ile Ser Thr 
65                  70                  75                  80  


Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr Pro Gly Lys 
                85                  90                  95      


Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu Val Ala Thr 
            100                 105                 110         


Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr Val Asn Gly 
        115                 120                 125             


Lys Ala Thr Lys Gly Asp Ala His Ile Gly Ser Gly Asp Ile Ile Lys 
    130                 135                 140                 


Leu Leu Asn Glu Gln Val Asn Lys Glu Met Gln Ser Ser Asn Leu Tyr 
145                 150                 155                 160 


Met Ser Met Ser Ser Trp Cys Tyr Thr His Ser Leu Asp Gly Ala Gly 
                165                 170                 175     


Leu Phe Leu Phe Asp His Ala Ala Glu Glu Tyr Glu His Ala Lys Lys 
            180                 185                 190         


Leu Ile Ile Phe Leu Asn Glu Asn Asn Val Pro Val Gln Leu Thr Ser 
        195                 200                 205             


Ile Ser Ala Pro Glu His Lys Phe Glu Gly Leu Thr Gln Ile Phe Gln 
    210                 215                 220                 


Lys Ala Tyr Glu His Glu Gln His Ile Ser Glu Ser Ile Asn Asn Ile 
225                 230                 235                 240 


Val Asp His Ala Ile Lys Ser Lys Asp His Ala Thr Phe Asn Phe Leu 
                245                 250                 255     


Gln Trp Tyr Val Ala Glu Gln His Glu Glu Glu Val Leu Phe Lys Asp 
            260                 265                 270         


Ile Leu Asp Lys Ile Glu Leu Ile Gly Asn Glu Asn His Gly Leu Tyr 
        275                 280                 285             


Leu Ala Asp Gln Tyr Val Lys Gly Ile Ala Lys Ser Arg Lys Ser His 
    290                 295                 300                 


His His His His His 
305                 


