                         SEQUENCE LISTING

<110>  Moe, Gregory R
       Giuntini, Serena
 
<120>  SARS-COV-2 RECEPTOR BINDING DOMAIN IN NATIVE OUTER MEMBRANE 
       VESICLES

<130>  OMV0001-401-PC

<150>  63/059,031
<151>  2020-07-30

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C Chain C, Spike glycoprotein receptor binding domain

<400>  1

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Ser 
    210                 215                 220                 


Gly 
225 


<210>  2
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal sequence for the spike glycoprotein, Chain A of SARS-CoV-2

<400>  2

Met Gly Ile Leu Pro Ser Pro Gly Met Pro Ala Leu Leu Ser Leu Val 
1               5                   10                  15      


Ser Leu Leu 
            


<210>  3
<211>  1253
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mature spike glycoprotein, Chain A of SARS-CoV-2

<400>  3

Ser Val Leu Leu Met Gly Cys Val Ala Glu Thr Gly Thr Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Glu Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ser Gly Ala Gly Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Gly Ser Gly Arg  Glu Asn Leu 
    1205                 1210                 1215             


Tyr Phe  Gln Gly Gly Gly Gly  Ser Gly Tyr Ile Pro  Glu Ala Pro 
    1220                 1225                 1230             


Arg Asp  Gly Gln Ala Tyr Val  Arg Lys Asp Gly Glu  Trp Val Leu 
    1235                 1240                 1245             


Leu Ser  Thr Phe Leu 
    1250             


<210>  4
<211>  976
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter+RBD

<400>  4
cctcagcgca tgcatccggg ctctgcgctt gaattgtgtt gtagaaacac aacgtttttg       60

aaaaaataag ctattgtttt atatcaaaat ataatcattt ttaaaataaa ggttgcggca      120

tttatcagat atttgttctg aaatgaagac gtatcgggtg tttgcccgat gtttttaggt      180

ttttatcaaa tttacaaaag gaagcccata tggtgaaccg aactgccttc tgcctttctc      240

tgaccgccgc cctgattctg accgcctgca gcagcggagg gggtggtcgc gtgcagccga      300

ccgaaagcat tgtgcgcttt ccgaacatta ccaacctgtg cccgtttggc gaagtgttta      360

acgcgacccg ctttgcgagc gtgtatgcgt ggaaccgcaa acgcattagc aactgcgtgg      420

cggattatag cgtgctgtat aacagcgcga gctttagcac ctttaaatgc tatggcgtga      480

gcccgaccaa actgaacgat ctgtgcttta ccaacgtgta tgcggatagc tttgtgattc      540

gcggcgatga agtgcgccag attgcgccgg gccagaccgg caaaattgcg gattataact      600

ataaactgcc ggatgatttt accggctgcg tgattgcgtg gaacagcaac aacctggata      660

gcaaagtggg cggcaactat aactatctgt atcgcctgtt tcgcaaaagc aacctgaaac      720

cgtttgaacg cgatattagc accgaaattt atcaggcggg cagcaccccg tgcaacggcg      780

tggaaggctt taactgctat tttccgctgc agagctatgg ctttcagccg accaacggcg      840

tgggctatca gccgtatcgc gtggtggtgc tgagctttga actgctgcat gcgccggcga      900

ccgtgtgcgg cccgaaaaaa agcaccaacc tggtgaaaaa caaatgcgtg aactttagcg      960

gctaaaggcc tgcagg                                                      976


<210>  5
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD25 produced in NOMV

<400>  5

Met Val Asn Arg Thr Ala Phe Cys Leu Ser Leu Thr Ala Ala Leu Ile 
1               5                   10                  15      


Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly Arg Val Gln Pro Thr Glu 
            20                  25                  30          


Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu 
        35                  40                  45              


Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys 
    50                  55                  60                  


Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala 
65                  70                  75                  80  


Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn 
                85                  90                  95      


Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly 
            100                 105                 110         


Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
        115                 120                 125             


Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp 
    130                 135                 140                 


Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu 
145                 150                 155                 160 


Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile 
                165                 170                 175     


Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu 
            180                 185                 190         


Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr 
        195                 200                 205             


Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu 
    210                 215                 220                 


Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn 
225                 230                 235                 240 


Leu Val Lys Asn Lys Cys Val Asn Phe Ser Gly 
                245                 250     


<210>  6
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD55 produced in NOMV

<400>  6

Met Asn Arg Thr Ala Phe Cys Leu Ser Leu Thr Ala Ala Leu Ile Leu 
1               5                   10                  15      


Thr Ala Cys Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Val Ala 
            20                  25                  30          


Ala Asp Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp 
        35                  40                  45              


His Lys Asp Lys Gly Leu Gln Arg Val Gln Pro Thr Glu Ser Ile Val 
    50                  55                  60                  


Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn 
65                  70                  75                  80  


Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser 
                85                  90                  95      


Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser 
            100                 105                 110         


Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys 
        115                 120                 125             


Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val 
    130                 135                 140                 


Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr 
145                 150                 155                 160 


Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn 
                165                 170                 175     


Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu 
            180                 185                 190         


Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu 
        195                 200                 205             


Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn 
    210                 215                 220                 


Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val 
225                 230                 235                 240 


Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His 
                245                 250                 255     


Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys 
            260                 265                 270         


Asn Lys Cys Val Asn Phe Ser Gly 
        275                 280 


<210>  7
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD expressed in mammalian cells

<400>  7

Met Glu Ile Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala Leu Glu Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn 
            20                  25                  30          


Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
        35                  40                  45              


Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
    50                  55                  60                  


Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
65                  70                  75                  80  


Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
                85                  90                  95      


Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
            100                 105                 110         


Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
        115                 120                 125             


Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
    130                 135                 140                 


Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
145                 150                 155                 160 


Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
                165                 170                 175     


Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
            180                 185                 190         


Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
        195                 200                 205             


Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
    210                 215                 220                 


Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
225                 230                 235                 240 


Asn Phe Gly Ser Gly His His His His His His 
                245                 250     


<210>  8
<211>  6683
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12 RBD plasmid

<400>  8
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcat aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccaat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cttataacag gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt tttttcagga tttaaaatta caaaaccctt acagagcaag     1440

taaacttgtt tgcttgttct gcaagggttc agcaaccgta gccgtcaggc gtagggcggt     1500

agcctataaa agccatttaa ttttatcttt aaacttcctt ttaaatgctt tgagtgggtg     1560

tcttttatcg tactcatcaa tccttttttg cattctttcg tttgctttgt gatcggcaaa     1620

ttttgaataa gatttttcca tctcatctaa cattctatca atccgttttt tatgttgcca     1680

tttcaggtaa acataaacac ttatagcaat taaagacaat atcaatacat tgtaaaaaat     1740

gattgttaca atttcgctca cagttatttt ttaccttttt caatttcttc attgataaat     1800

gcactcaatt catcaaattt cttgtcatca ttgataaatt tacgcaactt agggaagttt     1860

ctatctacat ctaaaagagg gttatttatt atttcattta gccaaaaagc ccctaataaa     1920

accttgtaat gcgtagcttt cttacgcttt tctgcttgtt cttttgactt aatcgcacga     1980

attttcgctt tgatttcgtc ctgcttgcgt tgtaaatctg cttgttgctg ttccaatctt     2040

gtaagttttt cgcttgccat actagcccct ttatatagtt agaaattatc gttattttat     2100

tcagtaggtg ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac     2160

ttatcagttt ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac     2220

tcaacactat ggcaatctat catttaaacg ttcgctattg cagtaaaagc aaagggcaat     2280

cagctcaagc caaaaacgac tacatcaacc gcaatgataa atattcaaag cggttagatg     2340

atttacagtt ttcaggctat ggtaatatgc caaaatttgc cgaagataat ccgcaagaat     2400

tttggcgatt gtcagatatt tacgagcgag ctaatgcccg agtttgtact gaaattgaat     2460

ttgctttacc tagagaatta accctagaac aacagcaaaa attagtaagt tcgtttatag     2520

aaaatacggt tgatagcggt agcaataaac taccctactc tttcgctatc cataccgata     2580

aaaataatca taatccccat tgtcatttga tattttcaga acgccaactt gacggcatag     2640

accgtacagc cgagcagttt tttaaacgtg ctaatactaa atccccagaa aagggcggag     2700

cgatgaaaac ggcagatttt cgagatcgtg agtttatcca atctgtccga aaaacgtgga     2760

gagagcaagc taatcaagcc ttagagcaat acggatatgc cgcacgaatt gacgaacgta     2820

gctacaagga acaaggcata gagcaagccc caagagcaag aattgacagg gtaacgtggc     2880

aagaattgaa ccgattagag caagaagaac gccaaatcgt gcaagagctt gcacttaaag     2940

gacaagaaat taacaaagaa aaatcctact tgcagaaaat cgaagaaaaa caggctcaag     3000

gaatgggcaa atatgaatcc aaattcgcag ctgcgttttc taaattatcg gaaagtgccc     3060

taaaacacga tttaagcaac gaaaaagaaa aagacagtaa aatacacact caagaagaaa     3120

aagtgcctca aaatcgcatt caggggcttt ctcaagcaga ttttgatcag tttttaattg     3180

atgaatggct acctcaaata gaaaaatacg ttaaagccca agaaaagcgg gacggaatgg     3240

aagtagagat cacgcaatac gacaaggatt tacagcgtat tcagggagac tataacaagc     3300

tcacagataa aaatcagggt tttctcggtt tatgggaaac taaagagcaa aaagcaaaga     3360

aaaaagagct tgaagatgaa tacaaacata cagcagagca acggaacgct aaaagccaag     3420

aattagccga gtatagccaa aaaataaaag catacgaaca gaaaacgcta gagccaatca     3480

acgagaagat tgccaaatat caagctgaca accctgaaat aaaaatgcgg agcttaggat     3540

ttgtgaaaaa aattaaggct caaggggcat ataaagcggc tcaagagcga atggagcgag     3600

aaaaacagca ccaacaggaa aaacaacaga gacatttaga gcgagagagt ggtttgagct     3660

tgtagctaac gccctacgcc tacggcttcg gttgttcaac ccttaaagaa ctcgcaacaa     3720

gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct aaacatttct gcccagcggt     3780

tgaaaattta cctattcacc attacaatga tcaagcagga aatttttttg attgccgtaa     3840

atgtccgtat atctagttga ggcacaaccc gccaaagtca ttgccccaac cagaacggcg     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggctc tgcgcttgaa ttgtgttgta gaaacacaac gtttttgaaa     4200

aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt     4260

atcagatatt tgttctgaaa tgaagacgta tcgggtgttt gcccgatgtt tttaggtttt     4320

tatcaaattt acaaaaggaa gcccatatgg tgaaccgaac tgccttctgc ctttctctga     4380

ccgccgccct gattctgacc gcctgcagca gcggaggggg tggtcgcgtg cagccgaccg     4440

aaagcattgt gcgctttccg aacattacca acctgtgccc gtttggcgaa gtgtttaacg     4500

cgacccgctt tgcgagcgtg tatgcgtgga accgcaaacg cattagcaac tgcgtggcgg     4560

attatagcgt gctgtataac agcgcgagct ttagcacctt taaatgctat ggcgtgagcc     4620

cgaccaaact gaacgatctg tgctttacca acgtgtatgc ggatagcttt gtgattcgcg     4680

gcgatgaagt gcgccagatt gcgccgggcc agaccggcaa aattgcggat tataactata     4740

aactgccgga tgattttacc ggctgcgtga ttgcgtggaa cagcaacaac ctggatagca     4800

aagtgggcgg caactataac tatctgtatc gcctgtttcg caaaagcaac ctgaaaccgt     4860

ttgaacgcga tattagcacc gaaatttatc aggcgggcag caccccgtgc aacggcgtgg     4920

aaggctttaa ctgctatttt ccgctgcaga gctatggctt tcagccgacc aacggcgtgg     4980

gctatcagcc gtatcgcgtg gtggtgctga gctttgaact gctgcatgcg ccggcgaccg     5040

tgtgcggccc gaaaaaaagc accaacctgg tgaaaaacaa atgcgtgaac tttagcggct     5100

aaaggcctac tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gattaagtgc     5160

ggtcatcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc ctacgtgctg     5220

ctgaagttgc cccaacagag agtggaacca accggtgata ccacgatact atgactgaga     5280

gtcaacgcca tgagcggcct catttcttat tctgagttac aacagtccgc accgctgtcc     5340

ggtagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc     5400

tttatcatgc aactcgtagg acaggtgccg gcagcgccca acagtccccc ggccacgggg     5460

cctgccacca tacccacgcc gaaacaagcg ccctgcacca ttatgttccg gatctgcatc     5520

gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctaaccg     5580

tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg     5640

ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg     5700

tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg     5760

aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact     5820

tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc     5880

ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag     5940

ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc     6000

gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt     6060

aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata     6120

aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg     6180

tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaac gtttcagttt     6240

gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt     6300

tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg     6360

ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct     6420

gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac     6480

gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt     6540

ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat     6600

tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg     6660

cccagggctt cccggtatca aca                                             6683


<210>  9
<211>  5185
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18-Lpxl1KO-FHbp25RBD-KAN plasmid

<400>  9
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtgt atcgagatga      420

aatttatatt ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga      480

ttgccgacct gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg      540

aaatcaattt ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga      600

aacagcattt caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc      660

ctgccggacg tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc      720

tggcggcggg ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttctcgagg      780

cagtgactaa ctaggaggaa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac      840

tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata      900

agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga      960

ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg     1020

ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg     1080

ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg     1140

agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata     1200

cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg     1260

atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg     1320

attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag     1380

acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg     1440

gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg     1500

gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga     1560

aaataaaata ttatatttta ctggatgaat tgttttagta cccctcagcg catgcatccg     1620

ggctctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     1680

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     1740

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     1800

aggaagccca tatggtgaac cgaactgcct tctgcctttc tctgaccgcc gccctgattc     1860

tgaccgcctg cagcagcgga gggggtggtc gcgtgcagcc gaccgaaagc attgtgcgct     1920

ttccgaacat taccaacctg tgcccgtttg gcgaagtgtt taacgcgacc cgctttgcga     1980

gcgtgtatgc gtggaaccgc aaacgcatta gcaactgcgt ggcggattat agcgtgctgt     2040

ataacagcgc gagctttagc acctttaaat gctatggcgt gagcccgacc aaactgaacg     2100

atctgtgctt taccaacgtg tatgcggata gctttgtgat tcgcggcgat gaagtgcgcc     2160

agattgcgcc gggccagacc ggcaaaattg cggattataa ctataaactg ccggatgatt     2220

ttaccggctg cgtgattgcg tggaacagca acaacctgga tagcaaagtg ggcggcaact     2280

ataactatct gtatcgcctg tttcgcaaaa gcaacctgaa accgtttgaa cgcgatatta     2340

gcaccgaaat ttatcaggcg ggcagcaccc cgtgcaacgg cgtggaaggc tttaactgct     2400

attttccgct gcagagctat ggctttcagc cgaccaacgg cgtgggctat cagccgtatc     2460

gcgtggtggt gctgagcttt gaactgctgc atgcgccggc gaccgtgtgc ggcccgaaaa     2520

aaagcaccaa cctggtgaaa aacaaatgcg tgaactttag cggctaaagg cctgcaggga     2580

ataatgagtc gacaggattt cggacgcaac gattcggttt ttgtggattt tttcggtatt     2640

cagacggcaa cgattaccgg attgagccgc attgccgcgc ttgcaaatgc aaaagtgata     2700

cccgccattc ccgtccgcga ggcagacaat acggttacat tgcatttcta ccctgcttgg     2760

aaatcctttc cgggtgaaga cgcgaaagcc gacgcgcagc gcatgaaccg ttttatcgaa     2820

gacagggtgc gcgaacatcc ggaacaatat ttttggctgc acaagcgttt taaaacccgt     2880

ccggaaggca gccccgattt ttactgacta cgtcagacgg ctctagagga tccccgggta     2940

ccgagctcga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     3000

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     3060

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     3120

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     3180

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     3240

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     3300

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     3360

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     3420

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     3480

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     3540

ggaagcgtgg cgctttctca aagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     3600

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     3660

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     3720

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     3780

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     3840

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     3900

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     3960

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     4020

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     4080

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     4140

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     4200

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     4260

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     4320

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     4380

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     4440

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     4500

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     4560

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     4620

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     4680

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     4740

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     4800

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     4860

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     4920

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     4980

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     5040

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     5100

cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat     5160

aggcgtatca cgaggccctt tcgtc                                           5185


<210>  10
<211>  6404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM-SiaD-GalE-FHbp25RBD-SPC plasmid

<400>  10
gggcgaattc ctggacacgg cagaccgata aagcccgcac cgccggtaac gaggatggtt       60

tttttgtttg cggtttgcat ttaatcccca tatccgttgg ggttgcggct gacccaacgc      120

cacgaatctt ccatcatttg ctgcaagccg cgtttggttt cccagccggt ttgttgtttg      180

gtatgggacg ggtcggcaaa ggaacacgcc aaatcaccgg cgcggcgcgg tttgatttca      240

aatggaatgg ttaaacccga agccgcttca aatgcgcgga tgatttccaa taccgaagaa      300

gcgcggccgg agcctaagtt cagcaaatgc gtgcctgcta cattactttt tgcctgcata      360

gccgcgacat ggccttctgc caaatccatc acatgaatat agtcacgcat ccccgtgccg      420

tcgggggtag ggtagtcatc gccaaatacc gccaattgcc tcagcgcatg catccgggct      480

ctgcgcttga attgtgttgt agaaacacaa cgtttttgaa aaaataagct attgttttat      540

atcaaaatat aatcattttt aaaataaagg ttgcggcatt tatcagatat ttgttctgaa      600

atgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga      660

agcccatatg gtgaaccgaa ctgccttctg cctttctctg accgccgccc tgattctgac      720

cgcctgcagc agcggagggg gtggtcgcgt gcagccgacc gaaagcattg tgcgctttcc      780

gaacattacc aacctgtgcc cgtttggcga agtgtttaac gcgacccgct ttgcgagcgt      840

gtatgcgtgg aaccgcaaac gcattagcaa ctgcgtggcg gattatagcg tgctgtataa      900

cagcgcgagc tttagcacct ttaaatgcta tggcgtgagc ccgaccaaac tgaacgatct      960

gtgctttacc aacgtgtatg cggatagctt tgtgattcgc ggcgatgaag tgcgccagat     1020

tgcgccgggc cagaccggca aaattgcgga ttataactat aaactgccgg atgattttac     1080

cggctgcgtg attgcgtgga acagcaacaa cctggatagc aaagtgggcg gcaactataa     1140

ctatctgtat cgcctgtttc gcaaaagcaa cctgaaaccg tttgaacgcg atattagcac     1200

cgaaatttat caggcgggca gcaccccgtg caacggcgtg gaaggcttta actgctattt     1260

tccgctgcag agctatggct ttcagccgac caacggcgtg ggctatcagc cgtatcgcgt     1320

ggtggtgctg agctttgaac tgctgcatgc gccggcgacc gtgtgcggcc cgaaaaaaag     1380

caccaacctg gtgaaaaaca aatgcgtgaa ctttagcggc taaaggtcta gagacgatgc     1440

cgtctgaaga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg acttcgctgc     1500

tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga     1560

cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt     1620

ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt     1680

atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg     1740

ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc     1800

ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg actcaactat     1860

cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt     1920

acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg     1980

tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt     2040

cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg     2100

acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc     2160

gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct     2220

tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct     2280

ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga     2340

actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt     2400

ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg     2460

agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac     2520

aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga     2580

aaggcgagat caccaaggta gtcggcaaat aatgtctatg ccgtctgaat actcgagcgg     2640

aacccgggta gtggcctgta aaagacagca tatagagatg agcaggctgt ataatattaa     2700

ggattttttt gtaacttcta taaatataaa gtaatttttt aggagttata ttattagggc     2760

ttctaggaag ctcaaataga taaatagatt caaatagatt cttgttagct gattgatgaa     2820

ctaacttagg catttttaag tttttagaag tatataaaat tactagtaaa ttattggtta     2880

atttttgtat tttaattagg ctttggactt ggttaagctg acctaaatta gatatgacaa     2940

ataaattgtt acgtgggggg gtaagataaa atggagatgt tgtcaaccac attgaatctt     3000

gaaaaaactt tttaggctga aaaagagctt tttttatttt ctttagcatt attgtatctc     3060

ttaaaaatta atgagaatta gctatatgta atagccaatc ctctgttaat aaagtaacta     3120

agttaataag cattattcaa tatcagtttt tttgatttga gcacctttgc gaatattgca     3180

agcagcgacc ttaccaaata atgtttcata ttcgttgacg ctgaagtctc cattgcctgg     3240

gcgtttaacc cataggttat ctaagcttga gtattctata gtgtcaccta aatagcttgg     3300

cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca     3360

acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca     3420

cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc     3480

attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt     3540

cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact     3600

caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag     3660

caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata     3720

ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc     3780

cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg     3840

ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc     3900

tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg     3960

gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc     4020

ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga     4080

ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg     4140

gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa     4200

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     4260

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt     4320

ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat     4380

tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct     4440

aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta     4500

tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa     4560

ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac     4620

gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa     4680

gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag     4740

taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg     4800

tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag     4860

ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg     4920

tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc     4980

ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat     5040

tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata     5100

ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa     5160

aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca     5220

actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc     5280

aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc     5340

tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg     5400

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac     5460

ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga     5520

ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc     5580

cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg     5640

cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg     5700

tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc     5760

gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat     5820

cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata     5880

gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt     5940

ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc     6000

atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa     6060

agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg     6120

gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt     6180

aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg     6240

ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg     6300

aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga     6360

cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tata                      6404


<210>  11
<211>  6146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBS-FHbpKO-FHbp25RBD-ERM plasmid

<400>  11
gcatgcatcc gggcagcagc gcatcggctc gcacgaggtc tgcgcttgaa ttgtgttgta       60

gaaacacaac gtttttgaaa aaataagcta ttgttttata tcaaaatata atcattttta      120

aaataaaggt tgcggcattt atcagatatt tgttctgaaa tgaagacgta tcgggtgttt      180

gcccgatgtt tttaggtttt tatcaaattt acaaaaggaa gcccatatgg tgaaccgaac      240

tgccttctgc ctttctctga ccgccgccct gattctgacc gcctgcagca gcggaggggg      300

tggtcgcgtg cagccgaccg aaagcattgt gcgctttccg aacattacca acctgtgccc      360

gtttggcgaa gtgtttaacg cgacccgctt tgcgagcgtg tatgcgtgga accgcaaacg      420

cattagcaac tgcgtggcgg attatagcgt gctgtataac agcgcgagct ttagcacctt      480

taaatgctat ggcgtgagcc cgaccaaact gaacgatctg tgctttacca acgtgtatgc      540

ggatagcttt gtgattcgcg gcgatgaagt gcgccagatt gcgccgggcc agaccggcaa      600

aattgcggat tataactata aactgccgga tgattttacc ggctgcgtga ttgcgtggaa      660

cagcaacaac ctggatagca aagtgggcgg caactataac tatctgtatc gcctgtttcg      720

caaaagcaac ctgaaaccgt ttgaacgcga tattagcacc gaaatttatc aggcgggcag      780

caccccgtgc aacggcgtgg aaggctttaa ctgctatttt ccgctgcaga gctatggctt      840

tcagccgacc aacggcgtgg gctatcagcc gtatcgcgtg gtggtgctga gctttgaact      900

gctgcatgcg ccggcgaccg tgtgcggccc gaaaaaaagc accaacctgg tgaaaaacaa      960

atgcgtgaac tttagcggct aaaggcctgc agggccaagc aaaaccattg tgaaaatgcc     1020

gtccgaacac gataatttac cgttcggacg gcattttgta ttgcaccgtc cgacggcatg     1080

cccaaggggg gaaatcccta ttttcaggcc aaccgctata taatgccgtc tgaaccaacg     1140

agagaatgcc atgcaagctg attttaaccg tcccgtcctg gccgtcgata ccggtacttc     1200

ccgtttgtcg ctcgcgctgc gtgccgacgg cgaaacccgt ctgttccatc aggaagtcgg     1260

cagccgccag tccgaactga ttctgccgga aatccgcacc ctattccgcg atgcaggcat     1320

taccgccgcc gatttgggtg cggtcgtgta cgcacagggt cccggcgcgt ttaccggact     1380

gcgtatcggc atcggtgtag ctcagggttt ggcaacgccg tttgataccc ccttaatcgg     1440

cgtaccctcg ctcgatgccg ccgcctcgct gccgccgccg caaagctgca tccttgccgc     1500

tacggacgct cgtatgggcg aagtgtttta tgcatggttc gatacgctgc tcgagggggg     1560

gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg     1620

gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc     1680

cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc     1740

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     1800

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     1860

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     1920

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     1980

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     2040

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     2100

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     2160

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     2220

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     2280

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     2340

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     2400

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     2460

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     2520

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     2580

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     2640

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     2700

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     2760

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     2820

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     2880

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     2940

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     3000

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     3060

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     3120

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     3180

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     3240

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     3300

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     3360

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     3420

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     3480

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     3540

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     3600

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     3660

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     3720

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta     3780

agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac     3840

caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg     3900

agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa     3960

gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt     4020

tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt     4080

agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga     4140

gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc     4200

gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg     4260

gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct     4320

gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg     4380

gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc     4440

cgctctagac cagccacggc gcatacaaat tcacccgtcc gcccacaggc gatgtattac     4500

gtatcgaccg catcaaagaa atccaccaag ccctgcccaa tacacacatc gtgatgcacg     4560

gctccagctc cgttccgcaa gaatggctga aagtcatcaa cgaatacggc ggcaatatcg     4620

gcgaaaccta cggcgtgccg gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca     4680

aagtcaacat cgataccgac ttgcgccttg cttctaccgg cgcggtacgc cgctaccttg     4740

ccgaaaatcc gtccgacttt gacccgcgca aatacctgag caaaaccatt gaggccatga     4800

agcaaatctg cctcgaccgt tatcttgcgt ttggctgcga aggtcaggca ggcaaaatca     4860

aacctgtttc gttggaaaaa atggcaagcc gttatgccaa gggcgaattg aaccaaatcg     4920

tcaaataaca ggttgcctgt aaacaaaatg ccgtcccatg ggcaaactta agagtgtgtt     4980

gatagtgcag tatcttaaaa ttttgtgtat aataggaatt gaagttaaat tagatgctaa     5040

aaatttgtaa ttaagaagga gggattcgtc atgttggtat tccaaatgcg taatgtagat     5100

aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa ataaatacgt     5160

tagattaatt cctaccagtg actaatctta tgacttttta aacagataac taaaattaca     5220

aacaaatcgt ttaacttctg tatttattta cagatgtaat cacttcagga gtaattacat     5280

gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat     5340

aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg     5400

gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag     5460

tcatctattc aacttatcgt cagaaaaatt aaaactgaac attcgtgtca ctttaattca     5520

ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat     5580

tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga     5640

catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact     5700

agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg     5760

ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga     5820

tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga     5880

atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa     5940

caatttaagt accattactt atgagcaagt attgtctatt tttaatagtt atctattatt     6000

taacgggagg aaataattct atgagtcgct tttttaaatt tggaaagtta cacgttacta     6060

aagggaatgg agataaatta ttagatatac tactgacagc ttccaagaag ctaaagaggt     6120

ccctttcgac ggccccgggc ctcagc                                          6146


<210>  12
<211>  5275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18-Lpxl1KO-FHbp55RBD-KAN plasmid

<400>  12
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtgt atcgagatga      420

aatttatatt ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga      480

ttgccgacct gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg      540

aaatcaattt ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga      600

aacagcattt caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc      660

ctgccggacg tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc      720

tggcggcggg ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttctcgagg      780

cagtgactaa ctaggaggaa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac      840

tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata      900

agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga      960

ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg     1020

ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg     1080

ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg     1140

agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata     1200

cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg     1260

atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg     1320

attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag     1380

acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg     1440

gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg     1500

gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga     1560

aaataaaata ttatatttta ctggatgaat tgttttagta cccctcagcg catgcatccg     1620

ggctctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     1680

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     1740

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     1800

aggaagccca tatggtgaac cgaactgcct tctgcctttc tctgaccgcc gccctgattc     1860

tgaccgcctg cagtagcgga ggcggcggaa gcggaggcgg cggtgtcgcc gccgacatcg     1920

gtgcggggct tgctgatgca ctaaccgcac cgctcgacca taaagacaaa ggtttgcagc     1980

gcgtgcagcc gaccgaaagc attgtgcgct ttccgaacat taccaacctg tgcccgtttg     2040

gcgaagtgtt taacgcgacc cgctttgcga gcgtgtatgc gtggaaccgc aaacgcatta     2100

gcaactgcgt ggcggattat agcgtgctgt ataacagcgc gagctttagc acctttaaat     2160

gctatggcgt gagcccgacc aaactgaacg atctgtgctt taccaacgtg tatgcggata     2220

gctttgtgat tcgcggcgat gaagtgcgcc agattgcgcc gggccagacc ggcaaaattg     2280

cggattataa ctataaactg ccggatgatt ttaccggctg cgtgattgcg tggaacagca     2340

acaacctgga tagcaaagtg ggcggcaact ataactatct gtatcgcctg tttcgcaaaa     2400

gcaacctgaa accgtttgaa cgcgatatta gcaccgaaat ttatcaggcg ggcagcaccc     2460

cgtgcaacgg cgtggaaggc tttaactgct attttccgct gcagagctat ggctttcagc     2520

cgaccaacgg cgtgggctat cagccgtatc gcgtggtggt gctgagcttt gaactgctgc     2580

atgcgccggc gaccgtgtgc ggcccgaaaa aaagcaccaa cctggtgaaa aacaaatgcg     2640

tgaactttag cggctaaagg cctgcaggga ataatgagtc gacaggattt cggacgcaac     2700

gattcggttt ttgtggattt tttcggtatt cagacggcaa cgattaccgg attgagccgc     2760

attgccgcgc ttgcaaatgc aaaagtgata cccgccattc ccgtccgcga ggcagacaat     2820

acggttacat tgcatttcta ccctgcttgg aaatcctttc cgggtgaaga cgcgaaagcc     2880

gacgcgcagc gcatgaaccg ttttatcgaa gacagggtgc gcgaacatcc ggaacaatat     2940

ttttggctgc acaagcgttt taaaacccgt ccggaaggca gccccgattt ttactgacta     3000

cgtcagacgg ctctagagga tccccgggta ccgagctcga attcgtaatc atggtcatag     3060

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc     3120

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc     3180

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     3240

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     3300

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     3360

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     3420

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     3480

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     3540

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     3600

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca aagctcacgc     3660

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     3720

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     3780

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     3840

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     3900

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     3960

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     4020

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     4080

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     4140

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     4200

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     4260

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     4320

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     4380

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     4440

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     4500

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt     4560

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     4620

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     4680

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     4740

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     4800

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     4860

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     4920

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     4980

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     5040

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     5100

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     5160

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc     5220

attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc          5275


<210>  13
<211>  6494
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM-SiaD-GalE-FHbp55RBD-SPC plasmid

<400>  13
gggcgaattc ctggacacgg cagaccgata aagcccgcac cgccggtaac gaggatggtt       60

tttttgtttg cggtttgcat ttaatcccca tatccgttgg ggttgcggct gacccaacgc      120

cacgaatctt ccatcatttg ctgcaagccg cgtttggttt cccagccggt ttgttgtttg      180

gtatgggacg ggtcggcaaa ggaacacgcc aaatcaccgg cgcggcgcgg tttgatttca      240

aatggaatgg ttaaacccga agccgcttca aatgcgcgga tgatttccaa taccgaagaa      300

gcgcggccgg agcctaagtt cagcaaatgc gtgcctgcta cattactttt tgcctgcata      360

gccgcgacat ggccttctgc caaatccatc acatgaatat agtcacgcat ccccgtgccg      420

tcgggggtag ggtagtcatc gccaaatacc gccaattgcc tcagcgcatg catccgggct      480

ctgcgcttga attgtgttgt agaaacacaa cgtttttgaa aaaataagct attgttttat      540

atcaaaatat aatcattttt aaaataaagg ttgcggcatt tatcagatat ttgttctgaa      600

atgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga      660

agcccatatg gtgaaccgaa ctgccttctg cctttctctg accgccgccc tgattctgac      720

cgcctgcagt agcggaggcg gcggaagcgg aggcggcggt gtcgccgccg acatcggtgc      780

ggggcttgct gatgcactaa ccgcaccgct cgaccataaa gacaaaggtt tgcagcgcgt      840

gcagccgacc gaaagcattg tgcgctttcc gaacattacc aacctgtgcc cgtttggcga      900

agtgtttaac gcgacccgct ttgcgagcgt gtatgcgtgg aaccgcaaac gcattagcaa      960

ctgcgtggcg gattatagcg tgctgtataa cagcgcgagc tttagcacct ttaaatgcta     1020

tggcgtgagc ccgaccaaac tgaacgatct gtgctttacc aacgtgtatg cggatagctt     1080

tgtgattcgc ggcgatgaag tgcgccagat tgcgccgggc cagaccggca aaattgcgga     1140

ttataactat aaactgccgg atgattttac cggctgcgtg attgcgtgga acagcaacaa     1200

cctggatagc aaagtgggcg gcaactataa ctatctgtat cgcctgtttc gcaaaagcaa     1260

cctgaaaccg tttgaacgcg atattagcac cgaaatttat caggcgggca gcaccccgtg     1320

caacggcgtg gaaggcttta actgctattt tccgctgcag agctatggct ttcagccgac     1380

caacggcgtg ggctatcagc cgtatcgcgt ggtggtgctg agctttgaac tgctgcatgc     1440

gccggcgacc gtgtgcggcc cgaaaaaaag caccaacctg gtgaaaaaca aatgcgtgaa     1500

ctttagcggc taaaggtcta gagacgatgc cgtctgaaga aaccttgcgc tcgttcgcca     1560

gccaggacag aaatgcctcg acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt     1620

ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat     1680

gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta     1740

acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc     1800

ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca     1860

gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag     1920

cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc     1980

tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc     2040

cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc     2100

gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc     2160

gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta     2220

agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc     2280

cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg     2340

ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg     2400

aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc     2460

gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc     2520

cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca     2580

tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag     2640

atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat     2700

aatgtctatg ccgtctgaat actcgagcgg aacccgggta gtggcctgta aaagacagca     2760

tatagagatg agcaggctgt ataatattaa ggattttttt gtaacttcta taaatataaa     2820

gtaatttttt aggagttata ttattagggc ttctaggaag ctcaaataga taaatagatt     2880

caaatagatt cttgttagct gattgatgaa ctaacttagg catttttaag tttttagaag     2940

tatataaaat tactagtaaa ttattggtta atttttgtat tttaattagg ctttggactt     3000

ggttaagctg acctaaatta gatatgacaa ataaattgtt acgtgggggg gtaagataaa     3060

atggagatgt tgtcaaccac attgaatctt gaaaaaactt tttaggctga aaaagagctt     3120

tttttatttt ctttagcatt attgtatctc ttaaaaatta atgagaatta gctatatgta     3180

atagccaatc ctctgttaat aaagtaacta agttaataag cattattcaa tatcagtttt     3240

tttgatttga gcacctttgc gaatattgca agcagcgacc ttaccaaata atgtttcata     3300

ttcgttgacg ctgaagtctc cattgcctgg gcgtttaacc cataggttat ctaagcttga     3360

gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt     3420

gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag     3480

cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt     3540

tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag     3600

gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     3660

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     3720

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     3780

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     3840

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     3900

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     3960

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     4020

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     4080

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     4140

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     4200

cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct     4260

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     4320

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     4380

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     4440

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     4500

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     4560

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     4620

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     4680

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     4740

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     4800

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     4860

acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat     4920

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag     4980

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac     5040

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt     5100

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt     5160

gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc     5220

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat     5280

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca     5340

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga     5400

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg     5460

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg     5520

ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga     5580

cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg     5640

acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg     5700

atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct     5760

ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa     5820

taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt     5880

gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat     5940

cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt     6000

ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt     6060

ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag     6120

gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg     6180

aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc     6240

gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc     6300

gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg     6360

cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt     6420

tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa     6480

tacgactcac tata                                                       6494


<210>  14
<211>  8275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBS-FHbpKO-FHbp55RBD-ERM plasmid

<400>  14
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttggatcc ttactagttc gcaatctacg agcttataac ctcgtttttt caattcattt      180

aaaaaatcag attttgagcc taattttgat ctattgctat cgttacccgc tagaaatacc      240

cagtaattac gcaaatcttc attggtaact ttcgtaatat cggtgtaatg atcttcgagt      300

atttttaagc aatctctagc ccataaaccg tactcgtgat tgctcatctt agggttttgc      360

ttatcgagtt tgacgaactt cccatacttg tttttatgtg gaaatactgg ccgttttgca      420

acttcttcaa ttttttgagc tgttcgtttt ttactaccaa tcacaaaatt taaagagtga      480

atagtacgcc cacgcttgat ttgttcaacc tcaacgacta aatcagattt ctcgttaatc      540

tcagttattg caggttccaa aacacgttga tttaatgaat taaatctagg gtatttattt      600

tcaacctgaa gccattcttt tagtttttct actgtaattt cacgactacc aacagagcga      660

tattgtgtaa ttagctcata aattcgaatt gaatgtacac tgttgaaata agcgatatgt      720

ttgagttgat attgcgtgaa ttgcccttta agttgcgtta ggtatggcat aacttcatca      780

gtcattgcaa ttctaaaacg cccctctttc ttgaaatatg ttctagagga aacccaacga      840

aattcagtta cacggtcttt atcttcagtt ttaacacttc ggtcgtaaat ccgttttata      900

gccgcctgaa tttgcttata ggcgttatct tggcttattt ctggaaactc acggacaaaa      960

tcagccaccg taaaatcaaa aatcttttga ttagatttcg gatccatagt cccgatagtt     1020

aaagctaaaa ttctgatttc atcaatactc aatcggtaat tggcttcaat aaggctatta     1080

gcctttacaa caactaaatc atttggcata atgccgtctg aaagacaaca aatttcctgt     1140

ttaaaacaac aagcaaaata tacctgttgt ttatatataa aacaacaagt attttcttaa     1200

aagttgtcta taacaggaaa tttgttgtcg tataacagga aatttgttgt cttataacag     1260

gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata agtttgtaac ttattgattt     1320

tactggtttt aaaaacgccg aaaacaagta aaaaacaaaa atataaaaat atagggactt     1380

tcgtcccttt tttgggcttt cagccctaat tttttctttt ttcaggatta aaaattacaa     1440

aacccttaca gagcaagtaa acttgtttgc ttgttctgca agggttcagc aaccgtagcc     1500

gtcaggcgta gggcggtagc ctataaaagc catttaattt tatctttaaa cttcctttta     1560

aatgctttga gtgggtgtct tttatcgtac tcatcaatcc ttttttgcat tctttcgttt     1620

gctttgtgat cggcaaattt tgaataagat ttttccatct catctaacat tctatcaatc     1680

cgttttttat gttgccattt caggtaaaca taaacactta tagcaattaa agacaatatc     1740

aatacattgt aaaaaatgat tgttacaatt tcgctcacag atgccgtctg aacccgggat     1800

gccgtctgaa actagcccct ttatatagtt agaaattatc gttattttat tcagtaggtg     1860

ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac ttatcagttt     1920

ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac tcaacactat     1980

gccgtctgaa ctcgagatgg caatctatca tttaaacgtt cgctattgca gtaaaagcaa     2040

agggcaatca gctcaagcca aaaacgacta catcaaccgc aatgataaat attcaaagcg     2100

gttagatgat ttacagtttt caggctatgg taatatgcca aaatttgccg aagataatcc     2160

gcaagaattt tggcgattgt cagatattta cgagcgagct aatgcccgag tttgtactga     2220

aattgaattt gctttaccta gagaattaac cctagaacaa cagcaaaaat tagtaagttc     2280

gtttatagaa aatacggttg atagcggtag caataaacta ccctactctt tcgctatcca     2340

taccgataaa aataatcata atccccattg tcatttgata ttttcagaac gccaacttga     2400

cggcatagac cgtacagccg agcagttttt taaacgtgct aatactaaat ccccagaaaa     2460

gggcggagcg atgaaaacgg cagattttcg agatcgtgag tttatccaat ctgtccgaaa     2520

aacgtggaga gagcaagcta atcaagcctt agagcaatac ggatatgccg cacgaattga     2580

cgaacgtagc tacaaggaac aaggcataga gcaagcccca agagcaagaa ttgacagggt     2640

aacgtggcaa gaattgaacc gattagagca agaagaacgc caaatcgtgc aagagcttgc     2700

acttaaagga caagaaatta acaaagaaaa atcctacttg cagaaaatcg aagaaaaaca     2760

ggctcaagga atgggcaaat atgaatccaa attcgcagct gcgttttcta aattatcgga     2820

aagtgcccta aaacacgatt taagcaacga aaaagaaaaa gacagtaaaa tacacactca     2880

agaagaaaaa gtgcctcaaa atcgcattca ggggctttct caagcagatt ttgatcagtt     2940

tttaattgat gaatggctac ctcaaataga aaaatacgtt aaagcccaag aaaagcggga     3000

cggaatggaa gtagagatca cgcaatacga caaggattta cagcgtattc agggagacta     3060

taacaagctc acagataaaa atcagggttt tctcggttta tgggaaacta aagagcaaaa     3120

agcaaagaaa aaagagcttg aagatgaata caaacataca gcagagcaac ggaacgctaa     3180

aagccaagaa ttagccgagt atagccaaaa aataaaagca tacgaacaga aaacgctaga     3240

gccaatcaac gagaagattg ccaaatatca agctgacaac cctgaaataa aaatgcggag     3300

cttaggattt gtgaaaaaaa ttaaggctcg aggggcatat aaagcggctc aagagcgaat     3360

ggagcgagaa aaacagcacc aacaggaaaa acaacagaga catttagagc gagagagtgg     3420

tttgagcttg tagatgccgt ctgaactaac gccctacgcc tacggcttcg gttgttcaac     3480

ccttaaagaa ctcgcaacaa gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct     3540

aaacatttct gcccagcggt tgaaaattta cctattcacc attacaatga tcaagcagga     3600

aatttttttg attgccgtaa atgtccgtat atctagttga ggcacaaccc gccaaagtca     3660

ttgccccaac cagaacggcg aaagctaata cgcaaaccgc ctctccccgc gcgttggccg     3720

attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac     3780

gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg     3840

gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgggaaa cagctatgac     3900

catgattacg ccaagcttcc tcagcgcatg catccgggca gcagcgcatc ggctcgcacg     3960

aggtctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     4020

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     4080

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     4140

aggaagccca tatgctctat cgaaggacct gcagggcctg aaataacctc tgaaagagga     4200

acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg     4260

tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag     4320

tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     4380

catctcaatt agtcagcaac catagtccca ctagtgctcc ggtgcccgtc agtgggcaga     4440

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc     4500

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     4560

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     4620

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     4680

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     4740

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     4800

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     4860

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgcagtta     4920

cagatccaag ctgtgaccgg cgcctacctg agatcaccgg tcaccatgga gatcaaggtg     4980

ctgtttgccc tcatctgtat tgctgttgct gaggcactcg agcgggtcca acccaccgaa     5040

agcattgtgc ggttcccaaa tatcaccaat ctgtgtccct ttggcgaagt gttcaatgct     5100

acaaggtttg cttctgtgta cgcatggaat aggaaacgca tctccaattg tgtcgctgat     5160

tactccgtgc tgtacaattc cgcctctttc tcaaccttca agtgttatgg cgtttcacct     5220

accaaactta acgacctgtg cttcactaat gtgtatgccg actcttttgt gatacgaggc     5280

gatgaagtga gacagattgc accagggcag accggcaaaa ttgccgacta caactacaag     5340

cttccagatg actttaccgg atgtgttatt gcatggaact caaacaatct ggattccaag     5400

gtgggtggca actataacta cctgtataga ctgttcagga aatccaacct gaaaccattc     5460

gagcgagata taagcacaga aatctaccag gctggaagta cgccctgcaa cggcgtggaa     5520

gggttcaact gctacttccc attgcagagt tacggattcc agcctacaaa cggggtgggt     5580

taccaaccct atcgtgtcgt agtcctgagt tttgagctcc tccatgcccc agccacagtc     5640

tgtggcccca agaaaagcac caatctggtg aagaacaaat gcgtgaactt tggatccggc     5700

catcatcatc accatcacta aagctagctg gccagacatg ataagataca ttgatgagtt     5760

tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc     5820

tattgcttta tttgtaacca ttataagctg caataaacaa gttaggcctg caggactagt     5880

cggccgtacc tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     5940

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     6000

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     6060

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     6120

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     6180

agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc     6240

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     6300

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     6360

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     6420

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     6480

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     6540

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     6600

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     6660

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg ggccctttcg     6720

tctcgcgcgt ttcggtgata gcttaagtgc ggtcatcttc ggtttccgtg tttcgtaaag     6780

tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc     6840

aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta     6900

ttctgagtta caacagtccg caccgctgcc ggtagctcct tccggtgggc gcggggcatg     6960

actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg     7020

gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg     7080

ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca     7140

cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa     7200

tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat     7260

ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga     7320

cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga     7380

atttctgcca ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg     7440

gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg     7500

taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat     7560

cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg     7620

ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg     7680

attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc     7740

accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta     7800

ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg     7860

aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc     7920

attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt     7980

tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc     8040

aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt     8100

atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc     8160

aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg     8220

ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caaca          8275


<210>  15
<211>  6838
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12 SV40 RBD-2 plasmid

<400>  15
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttggatcc ttactagttc gcaatctacg agcttataac ctcgtttttt caattcattt      180

aaaaaatcag attttgagcc taattttgat ctattgctat cgttacccgc tagaaatacc      240

cagtaattac gcaaatcttc attggtaact ttcgtaatat cggtgtaatg atcttcgagt      300

atttttaagc aatctctagc ccataaaccg tactcgtgat tgctcatctt agggttttgc      360

ttatcgagtt tgacgaactt cccatacttg tttttatgtg gaaatactgg ccgttttgca      420

acttcttcaa ttttttgagc tgttcgtttt ttactaccaa tcacaaaatt taaagagtga      480

atagtacgcc cacgcttgat ttgttcaacc tcaacgacta aatcagattt ctcgttaatc      540

tcagttattg caggttccaa aacacgttga tttaatgaat taaatctagg gtatttattt      600

tcaacctgaa gccattcttt tagtttttct actgtaattt cacgactacc aacagagcga      660

tattgtgtaa ttagctcata aattcgaatt gaatgtacac tgttgaaata agcgatatgt      720

ttgagttgat attgcgtgaa ttgcccttta agttgcgtta ggtatggcat aacttcatca      780

gtcattgcaa ttctaaaacg cccctctttc ttgaaatatg ttctagagga aacccaacga      840

aattcagtta cacggtcttt atcttcagtt ttaacacttc ggtcgtaaat ccgttttata      900

gccgcctgaa tttgcttata ggcgttatct tggcttattt ctggaaactc acggacaaaa      960

tcagccaccg taaaatcaaa aatcttttga ttagatttcg gatccatagt cccgatagtt     1020

aaagctaaaa ttctgatttc atcaatactc aatcggtaat tggcttcaat aaggctatta     1080

gcctttacaa caactaaatc atttggcata atgccgtctg aaagacaaca aatttcctgt     1140

ttaaaacaac aagcaaaata tacctgttgt ttatatataa aacaacaagt attttcttaa     1200

aagttgtcta taacaggaaa tttgttgtcg tataacagga aatttgttgt cttataacag     1260

gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata agtttgtaac ttattgattt     1320

tactggtttt aaaaacgccg aaaacaagta aaaaacaaaa atataaaaat atagggactt     1380

tcgtcccttt tttgggcttt cagccctaat tttttctttt ttcaggatta aaaattacaa     1440

aacccttaca gagcaagtaa acttgtttgc ttgttctgca agggttcagc aaccgtagcc     1500

gtcaggcgta gggcggtagc ctataaaagc catttaattt tatctttaaa cttcctttta     1560

aatgctttga gtgggtgtct tttatcgtac tcatcaatcc ttttttgcat tctttcgttt     1620

gctttgtgat cggcaaattt tgaataagat ttttccatct catctaacat tctatcaatc     1680

cgttttttat gttgccattt caggtaaaca taaacactta tagcaattaa agacaatatc     1740

aatacattgt aaaaaatgat tgttacaatt tcgctcacag atgccgtctg aacccgggat     1800

gccgtctgaa actagcccct ttatatagtt agaaattatc gttattttat tcagtaggtg     1860

ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac ttatcagttt     1920

ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac tcaacactat     1980

gccgtctgaa ctcgagatgc cgtctgaact aacgccctac gcctacggct tcggttgttc     2040

aacccttaaa gaactcgcaa caagttgcaa attctttaag ggttcgcaat aaaaacaacc     2100

gctaaacatt tctgcccagc ggttgaaaat ttacctattc accattacaa tgatcaagca     2160

ggaaattttt ttgattgccg taaatgtccg tatatctagt tgaggcacaa cccgccaaag     2220

tcattgcccc aaccagaacg gcgaaagcta atacgcaaac cgcctctccc cgcgcgttgg     2280

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc     2340

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt     2400

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacggg aaacagctat     2460

gaccatgatt acgccaagct tcctcagcgc atgcatccgg gcagcagcgc atcggctcgc     2520

acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt ttttgaaaaa ataagctatt     2580

gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat cagatatttg     2640

ttctgaaatg aagacgtatc gggtgtttgc ccgatgtttt taggttttta tcaaatttac     2700

aaaaggaagc ccatatgctc tatcgaagga cctgcagggc ctgaaataac ctctgaaaga     2760

ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta     2820

gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat     2880

tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc     2940

atgcatctca attagtcagc aaccatagtc ccactagtgc tccggtgccc gtcagtgggc     3000

agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg     3060

tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct     3120

ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt     3180

tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc     3240

acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc     3300

tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc     3360

gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac     3420

gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgcag     3480

ttacagatcc aagctgtgac cggcgcctac ctgagatcac cggtcaccat ggagatcaag     3540

gtgctgtttg ccctcatctg tattgctgtt gctgaggcac tcgagcgggt ccaacccacc     3600

gaaagcattg tgcggttccc aaatatcacc aatctgtgtc cctttggcga agtgttcaat     3660

gctacaaggt ttgcttctgt gtacgcatgg aataggaaac gcatctccaa ttgtgtcgct     3720

gattactccg tgctgtacaa ttccgcctct ttctcaacct tcaagtgtta tggcgtttca     3780

cctaccaaac ttaacgacct gtgcttcact aatgtgtatg ccgactcttt tgtgatacga     3840

ggcgatgaag tgagacagat tgcaccaggg cagaccggca aaattgccga ctacaactac     3900

aagcttccag atgactttac cggatgtgtt attgcatgga actcaaacaa tctggattcc     3960

aaggtgggtg gcaactataa ctacctgtat agactgttca ggaaatccaa cctgaaacca     4020

ttcgagcgag atataagcac agaaatctac caggctggaa gtacgccctg caacggcgtg     4080

gaagggttca actgctactt cccattgcag agttacggat tccagcctac aaacggggtg     4140

ggttaccaac cctatcgtgt cgtagtcctg agttttgagc tcctccatgc cccagccaca     4200

gtctgtggcc ccaagaaaag caccaatctg gtgaagaaca aatgcgtgaa ctttggatcc     4260

ggccatcatc atcaccatca ctaaagctag ctggccagac atgataagat acattgatga     4320

gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga     4380

tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaggc ctgcaggact     4440

agtcggccgt acctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt     4500

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc     4560

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct     4620

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta     4680

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc     4740

ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac     4800

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct     4860

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat     4920

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg     4980

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa     5040

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg     5100

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga     5160

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc     5220

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtgggccctt     5280

tcgtctcgcg cgtttcggtg atagcttaag tgcggtcatc ttcggtttcc gtgtttcgta     5340

aagtctggaa acgcggaagt cccctacgtg ctgctgaagt tgcccgcaac agagagtgga     5400

accaaccggt gataccacga tactatgact gagagtcaac gccatgagcg gcctcatttc     5460

ttattctgag ttacaacagt ccgcaccgct gccggtagct ccttccggtg ggcgcggggc     5520

atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg     5580

ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa     5640

gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga     5700

acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg gaggcagaat     5760

aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg     5820

catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat     5880

agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt     5940

cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac caggcgttta     6000

agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg     6060

ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg     6120

aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac     6180

gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca     6240

gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt     6300

ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg     6360

gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg     6420

gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg     6480

agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt     6540

ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg     6600

agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt     6660

ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa     6720

ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac     6780

gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaaca       6838


<210>  16
<211>  8578
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12 SV-40 RBD-2 complete plasmid

<400>  16
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttggatcc ttactagttc gcaatctacg agcttataac ctcgtttttt caattcattt      180

aaaaaatcag attttgagcc taattttgat ctattgctat cgttacccgc tagaaatacc      240

cagtaattac gcaaatcttc attggtaact ttcgtaatat cggtgtaatg atcttcgagt      300

atttttaagc aatctctagc ccataaaccg tactcgtgat tgctcatctt agggttttgc      360

ttatcgagtt tgacgaactt cccatacttg tttttatgtg gaaatactgg ccgttttgca      420

acttcttcaa ttttttgagc tgttcgtttt ttactaccaa tcacaaaatt taaagagtga      480

atagtacgcc cacgcttgat ttgttcaacc tcaacgacta aatcagattt ctcgttaatc      540

tcagttattg caggttccaa aacacgttga tttaatgaat taaatctagg gtatttattt      600

tcaacctgaa gccattcttt tagtttttct actgtaattt cacgactacc aacagagcga      660

tattgtgtaa ttagctcata aattcgaatt gaatgtacac tgttgaaata agcgatatgt      720

ttgagttgat attgcgtgaa ttgcccttta agttgcgtta ggtatggcat aacttcatca      780

gtcattgcaa ttctaaaacg cccctctttc ttgaaatatg ttctagagga aacccaacga      840

aattcagtta cacggtcttt atcttcagtt ttaacacttc ggtcgtaaat ccgttttata      900

gccgcctgaa tttgcttata ggcgttatct tggcttattt ctggaaactc acggacaaaa      960

tcagccaccg taaaatcaaa aatcttttga ttagatttcg gatccatagt cccgatagtt     1020

aaagctaaaa ttctgatttc atcaatactc aatcggtaat tggcttcaat aaggctatta     1080

gcctttacaa caactaaatc atttggcata atgccgtctg aaagacaaca aatttcctgt     1140

ttaaaacaac aagcaaaata tacctgttgt ttatatataa aacaacaagt attttcttaa     1200

aagttgtcta taacaggaaa tttgttgtcg tataacagga aatttgttgt cttataacag     1260

gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata agtttgtaac ttattgattt     1320

tactggtttt aaaaacgccg aaaacaagta aaaaacaaaa atataaaaat atagggactt     1380

tcgtcccttt tttgggcttt cagccctaat tttttctttt ttcaggatta aaaattacaa     1440

aacccttaca gagcaagtaa acttgtttgc ttgttctgca agggttcagc aaccgtagcc     1500

gtcaggcgta gggcggtagc ctataaaagc catttaattt tatctttaaa cttcctttta     1560

aatgctttga gtgggtgtct tttatcgtac tcatcaatcc ttttttgcat tctttcgttt     1620

gctttgtgat cggcaaattt tgaataagat ttttccatct catctaacat tctatcaatc     1680

cgttttttat gttgccattt caggtaaaca taaacactta tagcaattaa agacaatatc     1740

aatacattgt aaaaaatgat tgttacaatt tcgctcacag atgccgtctg aacccgggtt     1800

attttttacc tttttcaatt tcttcattga taaatgcact caattcatca aatttcttgt     1860

catcattgat aaatttacgc aacttaggga agtttctatc tacatctaaa agagggctat     1920

ttattacttc atttagccaa aaagccccta ataaaacctt gtaatgcgta gctttcttac     1980

gcttttctgc ttgttctttt gacttaatcg cacgaatttt cgctttgatt tcgtcctgct     2040

tgcgttgtaa atctgcttgt tgctgttcca atcttgtaag tttttcgctt gccatcccgg     2100

gatgccgtct gaaactagcc cctttatata gttagaaatt atcgttattt tattcagtag     2160

gtgctaggct tgcaagtgtt ctgttcatta cgttaaaata acgtaatgcc cacttatcag     2220

tttctcttcg agaaactggt gggcaagcgt accgcttgac cgtttcgcaa tactcaacac     2280

tatgccgtct gaactcgaga tggcaatcta tcatttaaac gttcgctatt gcagtaaaag     2340

caaagggcaa tcagctcaag ccaaaaacga ctacatcaac cgcaatgata aatattcaaa     2400

gcggttagat gatttacagt tttcaggcta tggtaatatg ccaaaatttg ccgaagataa     2460

tccgcaagaa ttttggcgat tgtcagatat ttacgagcga gctaatgccc gagtttgtac     2520

tgaaattgaa tttgctttac ctagagaatt aaccctagaa caacagcaaa aattagtaag     2580

ttcgtttata gaaaatacgg ttgatagcgg tagcaataaa ctaccctact ctttcgctat     2640

ccataccgat aaaaataatc ataatcccca ttgtcatttg atattttcag aacgccaact     2700

tgacggcata gaccgtacag ccgagcagtt ttttaaacgt gctaatacta aatccccaga     2760

aaagggcgga gcgatgaaaa cggcagattt tcgagatcgt gagtttatcc aatctgtccg     2820

aaaaacgtgg agagagcaag ctaatcaagc cttagagcaa tacggatatg ccgcacgaat     2880

tgacgaacgt agctacaagg aacaaggcat agagcaagcc ccaagagcaa gaattgacag     2940

ggtaacgtgg caagaattga accgattaga gcaagaagaa cgccaaatcg tgcaagagct     3000

tgcacttaaa ggacaagaaa ttaacaaaga aaaatcctac ttgcagaaaa tcgaagaaaa     3060

acaggctcaa ggaatgggca aatatgaatc caaattcgca gctgcgtttt ctaaattatc     3120

ggaaagtgcc ctaaaacacg atttaagcaa cgaaaaagaa aaagacagta aaatacacac     3180

tcaagaagaa aaagtgcctc aaaatcgcat tcaggggctt tctcaagcag attttgatca     3240

gtttttaatt gatgaatggc tacctcaaat agaaaaatac gttaaagccc aagaaaagcg     3300

ggacggaatg gaagtagaga tcacgcaata cgacaaggat ttacagcgta ttcagggaga     3360

ctataacaag ctcacagata aaaatcaggg ttttctcggt ttatgggaaa ctaaagagca     3420

aaaagcaaag aaaaaagagc ttgaagatga atacaaacat acagcagagc aacggaacgc     3480

taaaagccaa gaattagccg agtatagcca aaaaataaaa gcatacgaac agaaaacgct     3540

agagccaatc aacgagaaga ttgccaaata tcaagctgac aaccctgaaa taaaaatgcg     3600

gagcttagga tttgtgaaaa aaattaaggc tcgaggggca tataaagcgg ctcaagagcg     3660

aatggagcga gaaaaacagc accaacagga aaaacaacag agacatttag agcgagagag     3720

tggtttgagc ttgtagatgc cgtctgaact aacgccctac gcctacggct tcggttgttc     3780

aacccttaaa gaactcgcaa caagttgcaa attctttaag ggttcgcaat aaaaacaacc     3840

gctaaacatt tctgcccagc ggttgaaaat ttacctattc accattacaa tgatcaagca     3900

ggaaattttt ttgattgccg taaatgtccg tatatctagt tgaggcacaa cccgccaaag     3960

tcattgcccc aaccagaacg gcgaaagcta atacgcaaac cgcctctccc cgcgcgttgg     4020

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc     4080

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt     4140

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacggg aaacagctat     4200

gaccatgatt acgccaagct tcctcagcgc atgcatccgg gcagcagcgc atcggctcgc     4260

acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt ttttgaaaaa ataagctatt     4320

gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat cagatatttg     4380

ttctgaaatg aagacgtatc gggtgtttgc ccgatgtttt taggttttta tcaaatttac     4440

aaaaggaagc ccatatgctc tatcgaagga cctgcagggc ctgaaataac ctctgaaaga     4500

ggaacttggt taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta     4560

gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat     4620

tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc     4680

atgcatctca attagtcagc aaccatagtc ccactagtgc tccggtgccc gtcagtgggc     4740

agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg     4800

tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct     4860

ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt     4920

tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc     4980

acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc     5040

tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc     5100

gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac     5160

gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgcag     5220

ttacagatcc aagctgtgac cggcgcctac ctgagatcac cggtcaccat ggagatcaag     5280

gtgctgtttg ccctcatctg tattgctgtt gctgaggcac tcgagcgggt ccaacccacc     5340

gaaagcattg tgcggttccc aaatatcacc aatctgtgtc cctttggcga agtgttcaat     5400

gctacaaggt ttgcttctgt gtacgcatgg aataggaaac gcatctccaa ttgtgtcgct     5460

gattactccg tgctgtacaa ttccgcctct ttctcaacct tcaagtgtta tggcgtttca     5520

cctaccaaac ttaacgacct gtgcttcact aatgtgtatg ccgactcttt tgtgatacga     5580

ggcgatgaag tgagacagat tgcaccaggg cagaccggca aaattgccga ctacaactac     5640

aagcttccag atgactttac cggatgtgtt attgcatgga actcaaacaa tctggattcc     5700

aaggtgggtg gcaactataa ctacctgtat agactgttca ggaaatccaa cctgaaacca     5760

ttcgagcgag atataagcac agaaatctac caggctggaa gtacgccctg caacggcgtg     5820

gaagggttca actgctactt cccattgcag agttacggat tccagcctac aaacggggtg     5880

ggttaccaac cctatcgtgt cgtagtcctg agttttgagc tcctccatgc cccagccaca     5940

gtctgtggcc ccaagaaaag caccaatctg gtgaagaaca aatgcgtgaa ctttggatcc     6000

ggccatcatc atcaccatca ctaaagctag ctggccagac atgataagat acattgatga     6060

gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga     6120

tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaggc ctgcaggact     6180

agtcggccgt acctgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt     6240

catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc     6300

ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct     6360

tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta     6420

ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc     6480

ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac     6540

ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct     6600

gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat     6660

aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg     6720

acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa     6780

gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg     6840

gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga     6900

cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc     6960

aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gtgggccctt     7020

tcgtctcgcg cgtttcggtg atagcttaag tgcggtcatc ttcggtttcc gtgtttcgta     7080

aagtctggaa acgcggaagt cccctacgtg ctgctgaagt tgcccgcaac agagagtgga     7140

accaaccggt gataccacga tactatgact gagagtcaac gccatgagcg gcctcatttc     7200

ttattctgag ttacaacagt ccgcaccgct gccggtagct ccttccggtg ggcgcggggc     7260

atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg     7320

ccggcagcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa     7380

gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga     7440

acacctacat ctgtattaac gaagcgctaa ccgtttttat caggctctgg gaggcagaat     7500

aaatgatcat atcgtcaatt attacctcca cggggagagc ctgagcaaac tggcctcagg     7560

catttgagaa gcacacggtc acactgcttc cggtagtcaa taaaccggta aaccagcaat     7620

agacataagc ggctatttaa cgaccctgcc ctgaaccgac gaccgggtcg aatttgcttt     7680

cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac caggcgttta     7740

agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat cgcagtactg     7800

ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg     7860

aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac     7920

gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca     7980

gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt     8040

ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg     8100

gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg     8160

gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg     8220

agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt     8280

ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg     8340

agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt     8400

ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa     8460

ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac     8520

gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaaca       8578


<210>  17
<211>  8275
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12 SV-40 RBD-2 mobC plasmid

<400>  17
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttggatcc ttactagttc gcaatctacg agcttataac ctcgtttttt caattcattt      180

aaaaaatcag attttgagcc taattttgat ctattgctat cgttacccgc tagaaatacc      240

cagtaattac gcaaatcttc attggtaact ttcgtaatat cggtgtaatg atcttcgagt      300

atttttaagc aatctctagc ccataaaccg tactcgtgat tgctcatctt agggttttgc      360

ttatcgagtt tgacgaactt cccatacttg tttttatgtg gaaatactgg ccgttttgca      420

acttcttcaa ttttttgagc tgttcgtttt ttactaccaa tcacaaaatt taaagagtga      480

atagtacgcc cacgcttgat ttgttcaacc tcaacgacta aatcagattt ctcgttaatc      540

tcagttattg caggttccaa aacacgttga tttaatgaat taaatctagg gtatttattt      600

tcaacctgaa gccattcttt tagtttttct actgtaattt cacgactacc aacagagcga      660

tattgtgtaa ttagctcata aattcgaatt gaatgtacac tgttgaaata agcgatatgt      720

ttgagttgat attgcgtgaa ttgcccttta agttgcgtta ggtatggcat aacttcatca      780

gtcattgcaa ttctaaaacg cccctctttc ttgaaatatg ttctagagga aacccaacga      840

aattcagtta cacggtcttt atcttcagtt ttaacacttc ggtcgtaaat ccgttttata      900

gccgcctgaa tttgcttata ggcgttatct tggcttattt ctggaaactc acggacaaaa      960

tcagccaccg taaaatcaaa aatcttttga ttagatttcg gatccatagt cccgatagtt     1020

aaagctaaaa ttctgatttc atcaatactc aatcggtaat tggcttcaat aaggctatta     1080

gcctttacaa caactaaatc atttggcata atgccgtctg aaagacaaca aatttcctgt     1140

ttaaaacaac aagcaaaata tacctgttgt ttatatataa aacaacaagt attttcttaa     1200

aagttgtcta taacaggaaa tttgttgtcg tataacagga aatttgttgt cttataacag     1260

gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata agtttgtaac ttattgattt     1320

tactggtttt aaaaacgccg aaaacaagta aaaaacaaaa atataaaaat atagggactt     1380

tcgtcccttt tttgggcttt cagccctaat tttttctttt ttcaggatta aaaattacaa     1440

aacccttaca gagcaagtaa acttgtttgc ttgttctgca agggttcagc aaccgtagcc     1500

gtcaggcgta gggcggtagc ctataaaagc catttaattt tatctttaaa cttcctttta     1560

aatgctttga gtgggtgtct tttatcgtac tcatcaatcc ttttttgcat tctttcgttt     1620

gctttgtgat cggcaaattt tgaataagat ttttccatct catctaacat tctatcaatc     1680

cgttttttat gttgccattt caggtaaaca taaacactta tagcaattaa agacaatatc     1740

aatacattgt aaaaaatgat tgttacaatt tcgctcacag atgccgtctg aacccgggat     1800

gccgtctgaa actagcccct ttatatagtt agaaattatc gttattttat tcagtaggtg     1860

ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac ttatcagttt     1920

ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac tcaacactat     1980

gccgtctgaa ctcgagatgg caatctatca tttaaacgtt cgctattgca gtaaaagcaa     2040

agggcaatca gctcaagcca aaaacgacta catcaaccgc aatgataaat attcaaagcg     2100

gttagatgat ttacagtttt caggctatgg taatatgcca aaatttgccg aagataatcc     2160

gcaagaattt tggcgattgt cagatattta cgagcgagct aatgcccgag tttgtactga     2220

aattgaattt gctttaccta gagaattaac cctagaacaa cagcaaaaat tagtaagttc     2280

gtttatagaa aatacggttg atagcggtag caataaacta ccctactctt tcgctatcca     2340

taccgataaa aataatcata atccccattg tcatttgata ttttcagaac gccaacttga     2400

cggcatagac cgtacagccg agcagttttt taaacgtgct aatactaaat ccccagaaaa     2460

gggcggagcg atgaaaacgg cagattttcg agatcgtgag tttatccaat ctgtccgaaa     2520

aacgtggaga gagcaagcta atcaagcctt agagcaatac ggatatgccg cacgaattga     2580

cgaacgtagc tacaaggaac aaggcataga gcaagcccca agagcaagaa ttgacagggt     2640

aacgtggcaa gaattgaacc gattagagca agaagaacgc caaatcgtgc aagagcttgc     2700

acttaaagga caagaaatta acaaagaaaa atcctacttg cagaaaatcg aagaaaaaca     2760

ggctcaagga atgggcaaat atgaatccaa attcgcagct gcgttttcta aattatcgga     2820

aagtgcccta aaacacgatt taagcaacga aaaagaaaaa gacagtaaaa tacacactca     2880

agaagaaaaa gtgcctcaaa atcgcattca ggggctttct caagcagatt ttgatcagtt     2940

tttaattgat gaatggctac ctcaaataga aaaatacgtt aaagcccaag aaaagcggga     3000

cggaatggaa gtagagatca cgcaatacga caaggattta cagcgtattc agggagacta     3060

taacaagctc acagataaaa atcagggttt tctcggttta tgggaaacta aagagcaaaa     3120

agcaaagaaa aaagagcttg aagatgaata caaacataca gcagagcaac ggaacgctaa     3180

aagccaagaa ttagccgagt atagccaaaa aataaaagca tacgaacaga aaacgctaga     3240

gccaatcaac gagaagattg ccaaatatca agctgacaac cctgaaataa aaatgcggag     3300

cttaggattt gtgaaaaaaa ttaaggctcg aggggcatat aaagcggctc aagagcgaat     3360

ggagcgagaa aaacagcacc aacaggaaaa acaacagaga catttagagc gagagagtgg     3420

tttgagcttg tagatgccgt ctgaactaac gccctacgcc tacggcttcg gttgttcaac     3480

ccttaaagaa ctcgcaacaa gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct     3540

aaacatttct gcccagcggt tgaaaattta cctattcacc attacaatga tcaagcagga     3600

aatttttttg attgccgtaa atgtccgtat atctagttga ggcacaaccc gccaaagtca     3660

ttgccccaac cagaacggcg aaagctaata cgcaaaccgc ctctccccgc gcgttggccg     3720

attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac     3780

gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg     3840

gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacgggaaa cagctatgac     3900

catgattacg ccaagcttcc tcagcgcatg catccgggca gcagcgcatc ggctcgcacg     3960

aggtctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     4020

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     4080

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     4140

aggaagccca tatgctctat cgaaggacct gcagggcctg aaataacctc tgaaagagga     4200

acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg     4260

tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag     4320

tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg     4380

catctcaatt agtcagcaac catagtccca ctagtgctcc ggtgcccgtc agtgggcaga     4440

gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc     4500

ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt     4560

tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg     4620

caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg     4680

cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc     4740

cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag     4800

accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct     4860

ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgcagtta     4920

cagatccaag ctgtgaccgg cgcctacctg agatcaccgg tcaccatgga gatcaaggtg     4980

ctgtttgccc tcatctgtat tgctgttgct gaggcactcg agcgggtcca acccaccgaa     5040

agcattgtgc ggttcccaaa tatcaccaat ctgtgtccct ttggcgaagt gttcaatgct     5100

acaaggtttg cttctgtgta cgcatggaat aggaaacgca tctccaattg tgtcgctgat     5160

tactccgtgc tgtacaattc cgcctctttc tcaaccttca agtgttatgg cgtttcacct     5220

accaaactta acgacctgtg cttcactaat gtgtatgccg actcttttgt gatacgaggc     5280

gatgaagtga gacagattgc accagggcag accggcaaaa ttgccgacta caactacaag     5340

cttccagatg actttaccgg atgtgttatt gcatggaact caaacaatct ggattccaag     5400

gtgggtggca actataacta cctgtataga ctgttcagga aatccaacct gaaaccattc     5460

gagcgagata taagcacaga aatctaccag gctggaagta cgccctgcaa cggcgtggaa     5520

gggttcaact gctacttccc attgcagagt tacggattcc agcctacaaa cggggtgggt     5580

taccaaccct atcgtgtcgt agtcctgagt tttgagctcc tccatgcccc agccacagtc     5640

tgtggcccca agaaaagcac caatctggtg aagaacaaat gcgtgaactt tggatccggc     5700

catcatcatc accatcacta aagctagctg gccagacatg ataagataca ttgatgagtt     5760

tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc     5820

tattgcttta tttgtaacca ttataagctg caataaacaa gttaggcctg caggactagt     5880

cggccgtacc tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat     5940

ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct     6000

taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct     6060

tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca     6120

gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc     6180

agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc     6240

aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct     6300

gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag     6360

gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc     6420

tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg     6480

agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag     6540

cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt     6600

gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac     6660

gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtg ggccctttcg     6720

tctcgcgcgt ttcggtgata gcttaagtgc ggtcatcttc ggtttccgtg tttcgtaaag     6780

tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc     6840

aaccggtgat accacgatac tatgactgag agtcaacgcc atgagcggcc tcatttctta     6900

ttctgagtta caacagtccg caccgctgcc ggtagctcct tccggtgggc gcggggcatg     6960

actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg     7020

gcagcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg     7080

ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca     7140

cctacatctg tattaacgaa gcgctaaccg tttttatcag gctctgggag gcagaataaa     7200

tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat     7260

ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaaac cagcaataga     7320

cataagcggc tatttaacga ccctgccctg aaccgacgac cgggtcgaat ttgctttcga     7380

atttctgcca ttcatccgct tattatcact tattcaggcg tagcaaccag gcgtttaagg     7440

gcaccaataa ctgccttaaa aaaattacgc cccgccctgc cactcatcgc agtactgttg     7500

taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat     7560

cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg     7620

ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg     7680

attggctgag acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc     7740

accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta     7800

ttcactccag agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg     7860

aacactatcc catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc     7920

attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt     7980

tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc     8040

aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt     8100

atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc     8160

aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg     8220

ccgatcaacg tctcattttc gccaaaagtt ggcccagggc ttcccggtat caaca          8275


<210>  18
<211>  259
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FHbp ID9

<400>  18

Cys Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Val Ala Ala Asp 
1               5                   10                  15      


Ile Gly Ala Gly Leu Ala Asp Ala Leu Thr Ala Pro Leu Asp His Lys 
            20                  25                  30          


Asp Lys Gly Leu Gln Ser Leu Thr Leu Asp Gln Ser Val Arg Lys Asn 
        35                  40                  45              


Glu Lys Leu Lys Leu Ala Ala Gln Gly Ala Glu Lys Thr Tyr Gly Asn 
    50                  55                  60                  


Gly Asp Ser Leu Asn Thr Gly Lys Leu Lys Asn Asp Lys Val Ser Arg 
65                  70                  75                  80  


Phe Asp Phe Ile Arg Gln Ile Glu Val Asp Gly Gln Leu Ile Thr Leu 
                85                  90                  95      


Glu Ser Gly Glu Phe Gln Val Tyr Lys Gln Ser His Ser Ala Leu Thr 
            100                 105                 110         


Ala Leu Gln Thr Glu Gln Val Gln Asp Ser Glu Asp Ser Gly Lys Met 
        115                 120                 125             


Val Ala Lys Arg Gln Phe Arg Ile Gly Asp Ile Ala Gly Glu His Thr 
    130                 135                 140                 


Ser Phe Asp Lys Leu Pro Lys Gly Gly Ser Ala Thr Tyr Arg Gly Thr 
145                 150                 155                 160 


Ala Phe Gly Ser Asp Asp Ala Gly Gly Lys Leu Thr Tyr Thr Ile Asp 
                165                 170                 175     


Phe Ala Val Lys Gln Gly His Gly Lys Ile Glu His Leu Lys Ser Pro 
            180                 185                 190         


Glu Leu Asn Val Asp Leu Ala Ala Ala Tyr Ile Lys Pro Asp Lys Lys 
        195                 200                 205             


Arg His Ala Val Ile Ser Gly Ser Val Leu Tyr Asn Gln Asp Glu Lys 
    210                 215                 220                 


Gly Ser Tyr Ser Leu Gly Ile Phe Gly Gly Gln Ala Gln Glu Val Ala 
225                 230                 235                 240 


Gly Ser Ala Glu Val Glu Thr Ala Asn Gly Ile His His Ile Gly Leu 
                245                 250                 255     


Ala Ala Lys 
            


