                         SEQUENCE LISTING

<110>  Biotalys N.V.
 
<120>  METHODS OF INCREASING RECOMBINANT PROTEIN YIELDS

<130>  P142471WO

<150>  EP20188933.4
<151>  2020-07-31

<150>  EP20188921.9
<151>  2020-07-31

<150>  EP20217907.3
<151>  2020-12-30

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH1 D

<400>  1

Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Arg Ser Ile Phe Ser Ile Asn 
            20                  25                  30          


Ala Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Trp Val 
        35                  40                  45              


Ala Gly Ile Thr Arg Gly Gly Thr Thr Lys Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys Lys Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Val Leu Arg Gly Glu Gln Pro Trp Thr Arg Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser 
        115                 


<210>  2
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH1 Q

<400>  2

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Arg Ser Ile Phe Ser Ile Asn 
            20                  25                  30          


Ala Met Asp Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Trp Val 
        35                  40                  45              


Ala Gly Ile Thr Arg Gly Gly Thr Thr Lys Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys Lys Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Val Leu Arg Gly Glu Gln Pro Trp Thr Arg Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser 
        115                 


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH1 CDR1

<400>  3

Arg Ser Ile Phe Ser Ile Asn Ala Met Asp 
1               5                   10  


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH1 CDR2

<400>  4

Gly Ile Thr Arg Gly Gly Thr Thr Lys 
1               5                   


<210>  5
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH1 CDR3

<400>  5

Leu Arg Gly Glu Gln Pro Trp Thr Arg Asp Tyr 
1               5                   10      


<210>  6
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH2 Q

<400>  6

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Thr Ile Phe Arg Pro Thr 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Thr Thr Gly Gly Ser Thr Lys Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Gly Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Gln Trp Gly Val Arg Thr Arg Asp Tyr Trp Gly Gln Gly Thr Gln 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  7
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH2 D

<400>  7

Asp Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Thr Ile Phe Arg Pro Thr 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Leu Val 
        35                  40                  45              


Ala Thr Ile Thr Thr Gly Gly Ser Thr Lys Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Gly Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Gln Trp Gly Val Arg Thr Arg Asp Tyr Trp Gly Gln Gly Thr Gln 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  8
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH2 CDR1

<400>  8

Gly Thr Ile Phe Arg Pro Thr Ala Met Gly 
1               5                   10  


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH2 CDR2

<400>  9

Thr Ile Thr Thr Gly Gly Ser Thr Lys 
1               5                   


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH2 CDR3

<400>  10

Gln Trp Gly Val Arg Thr Arg Asp Tyr 
1               5                   


<210>  11
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH3 Q

<400>  11

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Asp Arg Ala Phe Ser 
            20                  25                  30          


Arg His Val Met Gly Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Gly Trp Thr Gly Arg Arg Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Asn Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Ser His Phe Tyr Ser Val Ser Phe Glu Ile Asn Asp 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  12
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH3 D

<400>  12

Asp Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ile Ser Asp Arg Ala Phe Ser 
            20                  25                  30          


Arg His Val Met Gly Trp Phe Arg Gln Pro Pro Gly Lys Glu Arg Glu 
        35                  40                  45              


Phe Val Ala Ala Ile Gly Trp Thr Gly Arg Arg Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Asn Thr 
65                  70                  75                  80  


Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Ala Ala Ser His Phe Tyr Ser Val Ser Phe Glu Ile Asn Asp 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH3 CDR1

<400>  13

Ile Ser Asp Arg Ala Phe Ser Arg His Val 
1               5                   10  


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH3 CDR2

<400>  14

Ala Ile Gly Trp Thr Gly Arg Arg Thr Tyr 
1               5                   10  


<210>  15
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH3 CDR3

<400>  15

Ser His Phe Tyr Ser Val Ser Phe Glu Ile Asn Asp Tyr Asp Tyr 
1               5                   10                  15  


