                         SEQUENCE LISTING

<110>  Sandoz AG
 
<120>  Improved process for preparing clavulanic acid

<130>  HS 249-PD35904EP00

<160>  55    

<170>  PatentIn version 3.5

<210>  1
<211>  7779
<212>  DNA
<213>  Streptomyces clavuligerus

<400>  1
gtgtccggac cattgccctc gcctgtttcc gccggtcccc agggccccgt cggccctgcc       60

ctccccgtgg gctccacgga ccccgccggt cccgggagcc ccagcggagc cgcacactcc      120

gacacccttc cctgcgcctt cgcccggcag accgcgctca cccccgacgc gaccgcgctg      180

atctgcggcg acacccatct cacctatcga cggctcggtg cactcgccga ccggctggcg      240

gccctgctcg ctgccgaggg cgtgggcccc ggcaccaccg tggccgtacg gctggagcgc      300

tcggccgaac tcgtcgtctc cctgctcgcg atcgcgcggg cgggcgccgc ctatgtcccc      360

ctcgacctcc ggcagccggt ctcccgtacc gagtggatac tcaaggagac cggcgcggaa      420

ctcgtcctga cggccgcccc cgctcccgga cccggtaccg cgcccaccgc cgggccccgc      480

gccctgcccg tctccctcga cgccctcgcc gcgcgcggcc cgcatgccct ccggggctcc      540

cgcgacggcc tcggggaccc ggccgccggg gacgccccgg ccggggacgg cgacccgcgc      600

cgccccgcgt acgtgatgtt cacctccggc tccaccggta cgcccaaggg cgtcgcgacc      660

acccacggca atgtggtcgc cctggcccgt gacccgctct ggggcggcgg tgcgcacgac      720

cgcgtcctgc tccactcgcc gcacgcgttc gacgcctcca cctacgaact gtgggtgccc      780

ctgctgcgcg gcggcacggt cgtcgtcgcg ccccccggtg acctcgacgc ccgggcggtc      840

gccgcgctga ccgggcggta cgggatcacg ggactctggg tgaccgccgg gctgttcgcc      900

gtcctcgccg aggacgaccc gcgctgcttc gccgggctgc gcgaggtctg gaccggcggt      960

gacgtggtct ccccgaccgc ggtgcgccgg gtcctggccg cctgccccgg actcgccgtc     1020

gtcaacggct acggccccac cgagacgacc acgttcgcca cctgccaccg ggtggagcac     1080

acggacggcc tcggcaccgt ggtccccatc ggggcggcca tggcggggat gcggaccgtg     1140

gtcctcgaca ccgagctgcg cccggtggaa cccggcggga ccggcgaact ctacctgggc     1200

ggctcgggcc tggcccgcgg ctactggaac cggccggggc tgaccgcggg gcgctttgtc     1260

gccgaccccg tgggcgcgcc gggcgaacgg ctcttccgca ccggtgacct ggtgcggcgc     1320

ggcccggacg gattactgga gttcgtcgga cgcagcgacg accaggtgaa gatacgcggc     1380

ttccgggtgg agcccggcga ggcggaggcg gtgctcggcg accaccccgg ggtctcccgg     1440

gcggcggtcg tcgtccggga ggaccggccg ggcgacaagc gtctcgtcgg ctatgtcgtc     1500

cccgccgacg agaccccctc cacgacggac acggcccgcc gggactccgt cgccgagtgg     1560

cgcgccatct acgacacgct ctaccgcgac accgccggga ccggtctcgg cgaggacttc     1620

tccggctgga acagcagtta cgacggacag cccctgcccc tgccgcagat gcgcgcctgg     1680

cgcgacgcca ccgtggcccg ggtccgtgag ctggacccgc ggcgggtgct ggagatcggc     1740

gtcggcaccg gtctgctgat gtcgcggctg gcgccgcact gcgagagcta ctggggcacg     1800

gacctgtccg gcgaggtcat cgccgcgctg gcggacgccg tccgccgggc ccccggcctc     1860

gccgaccggg tggagctgcg cgtccgtacc gccgacgaca tcgagggcct gcccgccggg     1920

ttcttcgaca ccgtggtcgt caactccgtc gtccagtact tccccgacgc cgactacctc     1980

accgatgtcc tgaccaaggc cgtcggcctg ctggcccccg gcggggcggt cttcgtcggc     2040

gatgtgcgca atccacggct gctgcgggcc ttccacaccg atgtccggct ccgccgcccg     2100

gagcccggcg cccccctcga ccccgacgcc gtgcgcgccg ccgtcgaaca gggcatggtc     2160

cgggagaagg aactcctcgt cgaccccgcc tacttcaccg gactcgcgca caccctcgcc     2220

gacgccgtcg gcgccgaggt acgggtcaaa cggggccggt acgtcaatga gctgacccgc     2280

taccgctacg acgcggtcct gcgcaagggg ttccccgccg gggccgggtc cgacggccgc     2340

gccgacggcc ccgccccggc cgtacgggag ctgcgctggg gacaggacgt cacagacctc     2400

aaggatctca aggacgtcgg gggccccggc accgccgcgc acaccgagct gcgcatcacc     2460

ggtctgcccg accaccggct gacccccgtg gccgccgcgc tgcggacctt gaccgacggc     2520

gccagcccgg tcccctccct ctccggcgcg gacgacgccg actgcgccgg ggtggaggac     2580

ctccacaccc tcgccgaccg gctcggactg cgcgcctccg tctcctggtc gtccaccggc     2640

gagggcaccc tggacgccgt cctcacctcc cgtccccccg gcgccgtcac cgggcccgcc     2700

ccgggcacgc cgctctccgc gctggtcaac gccccgtcga cggcgcggga caccggggcg     2760

ctcatccgat ccgtccgctc cttcctcggt gcccggctgc ccgagtacct gacccccgac     2820

gcgttcgtcg tcctcgacgc cctgccgctg accccgcacg gaaaggtgga ccggcgccgg     2880

ctgccgcgcc cggactccgg ggccgccgag cacggacggg cgccgcgcga cccgatggag     2940

gagctgctgt gcgggctctt cgccgaggtg ctgggggcct cccgggtcag tgtcgacgac     3000

gacttcttcg ccctcggcgg gcactccctc tccgccaccc ggctcagcgg acggatacgc     3060

tccgccctcg gtctggaact gccgctgcgc agcgtcttcg agaccccgac ggtcgccgcg     3120

ctcgccacgg cggtacggcg ggccatcgcg ggggagcggc ccccgctgac cccggcggag     3180

cgcccgggga ccgtgccgct ctcgttcgcg cagcggcggc tgtggttcct gggccgcctc     3240

gactcccagg gcgccgccta ccacgtcccg ctggtgctgc ggctgacggg ggacgtggac     3300

cgccccgcgc tcgaagccgc gctcggcgat ctgctcgccc gtcacgagag cctgcggacc     3360

gtcttccccg agacggcggg cactccccgg cagcgggtgc tcgacggcgc cgcggcccgg     3420

ctcccgctca cctccgtgac cctcacggac ggggacggcg aggacgacgg ggcactgcac     3480

gcggccgtcg ccgaagccgt ccgccgcccc ttcgacctgg cggccgacct gcccgtccgg     3540

gcctggctgt tcacggccgc gccggaccgc catgtcctgg ccctggtcgt ccaccacatc     3600

gcctgcgacg gctggtcgct ggcgccgctg tggcgggacc tcgccgacgc gtacgcggcc     3660

cgcggcctcg gccgggctcc cggggcggcg ccgttgccgg tgcagtacgc ggactacacc     3720

ctgtggcagc ggcggctgct cggcgacccg gacgaccccg gcagcacggc cgcccggcag     3780

accgcctact ggcgcaccgc gctcgccggg ctccccgagt gcgccgtcct gcccgtcgac     3840

cggccccggc cgcagaccgc ctcccaccgg ggcgacaccc tccacttcac cgtcgacgcc     3900

gaactgtgcc gccgggtcgg ggagctgtcc gcgcggtacg gggtgagcac cttcatggtg     3960

ctgcacgcgg cgctcgccgc cctgctcacc cggctgggaa cgggcaccga cgtcgtgatc     4020

ggcaccccgg tcgcgggccg taccgacccc gcgctcgacg atgtcgtcgg cttcttcgtc     4080

aatatgctgg tcctgcggac ggacacctcg ggcgacccct ccttccggga gctgctcgcc     4140

cgggtgcggg agaccgacct cgccgcctac acccatcagg acgtgccctt cgaacggctg     4200

gtggacatcc tcgccccggc ccgcggcctg gaccgccacc ccctcttcca ggtgatgctg     4260

gcgctccaga acgccccccg ccagacaccc gaactgcccg gtctcaccgt cacggaacag     4320

gccgtcgaca ccggggcctg ccgcttcgac ctctcgctga gcctccggga gcgcccggcc     4380

ggagcggagc cgtcgggggc cggagccgtc ggcatggacg gggtggccga gtacagcacc     4440

gacctcttcg accgggacac ggtacggcgg ctcgtccggc gcttcctgct gctgctcgac     4500

gccgccaccg ccgagcccgg ccggcccctc ggcggcctgg acgtcctgct gcccgaggaa     4560

cgcggctggc tgctcgcgcg cggcggcgac agcgggcccg ccacccccgg caggccgctg     4620

cccgagctgt tcgaggcaca ggtgcgccgg acgcccgacg ccctcgcgct gctccacgac     4680

gacggcgcgg gccgggtcac gaccctgacc ttcgccgagg tgaacggggc ggcgaaccgg     4740

ctggcccggc agctcgtcgc ccgtggcgcg ggcccggagc ggatcgtcgc gctcgccctg     4800

ccccgcaccc ccgagctggt cgtcgcgatc ctcgccgtcc tcaaggcggg cgccgcctat     4860

ctgcccctcg accccgactg gcccgccgcc cggctgcgcc tcatggcgga cgacgcccgg     4920

ccggtcctgc tgctgacgca ctccgccgcg gggcccgggt ccctgaccgc cccggcggac     4980

cccgacgggc ccggcccgca cggggtgtac gtccccgaac ggatcgagct ggaccgggaa     5040

gccgtccgcc gggagaccgc ccggcaggac cccgcggacc tcgccgacag cgaccgcgtc     5100

cgccccctcg gctgcgccga ccccgcctat gtcatctaca cctccggctc caccggccgc     5160

cccaagggcg tcgtggtcac ccacacgggc ttcgtgggcg tcgtcgaggc acagcggcgg     5220

cggttcgggg tgacgccgtc gagccggata ctccagttcg cctcgcacag cttcgacgga     5280

gcggtgtggg agctgtgcgg ggggctgctg accggagcgg ccctggtcat ggcaccggcg     5340

gaggccaccg cccccggccc ccggctggcc tctctcgtcg cccggcacgg cgtcacccac     5400

gcgaccctgc cacccgccgc cctgacggtc atggagcccg atcaactgcc ctcgctcacc     5460

tcgatgatcg tctccggtga ggcggcctcc gaggagaccg tgcgccgctg gtcggcgggg     5520

cgcgtcctca tcaacggcta cggcccgacc gagaccaccg tgtgcgccac cctcagcgcc     5580

ccgctgtccg gcggcaccgc gccgcccatc ggccgtccca cggtcaacgc ccgcgcctat     5640

gtgctggatt ccgcgctgcg gctggtgccg cccggggtcc ccggcgagct gcacatctcc     5700

ggtgccgggc tggcccgggg ctatctccgc cggcccgcgc tgaccgccga gcgcttcgtc     5760

gccgacccct tcggcgcccc gggcacccgg atgtaccgga ccggcgacct cgcgcggtgg     5820

aaccccgaag gggagctgga gttcatcggc cgcgccgacg gacagctcaa gatccgcggt     5880

ttcaggatcg agccgggcga gatcgaggcg gcgctcggga cccacccggc ggtggcgcag     5940

accgtcgttc tgccccaccc ggacccgcgc ggcgacatct gcctggtcgc ctatgccgtg     6000

cccgccgccg acaccccggc ccccgggggc gccgaactcc gcgcccacct ggccgggatg     6060

ctccccgacc acctggttcc cgccgtcgtc ctggtcctgg acgccgtccc ggtcaccggg     6120

gcgggcaagg tcgaccgcgc cgcgctcccg gtccccgact tcggggcgct gtcggcgggc     6180

aggccccccg ccaccccgcg cgagcacgcc ctgtgcgcgc tgttcgcgga tgtcctgaac     6240

gttccttccg tcaccgtcga cgacaacttc ttcgccctgg gcggccactc gctgctggtg     6300

ccccgggtga tcagcgggat cgcgctgcgc ctcggggccg agctgcccat gcggacgctc     6360

ttcgagcggc agtcggtggc cgcgctcctg gaggccgtcg acgggaccgc ggcccccgac     6420

cccgcccacg gcggcgaggg cggcggggag gcacggcacg ggaacggtgc cccggtggga     6480

ccggcggcgg acgccgtgct cgaccccgag atccgggtac ggggcaccgg gcccgacccc     6540

tcggcgccgg tatcggtgcc ggacgccgcg ccgcgccgcc cccgtcgctc caccggcggc     6600

gagcacatcc tcctgaccgg cgcgaccggc ttcctcggcg ccttcctcct ccgggaactc     6660

ctcgaccgca ccgacgcgac cgtgcactgc ctggtccgcg ccgacggggc cgaggaggcg     6720

gcccggcgcg tccgggacgg cctcgaccgc ttcggcctgc gacgcgctca ggacccccag     6780

ggcacagggg gcacccagga cgccggggat ctcgacggcc gggtggtcgc cgtccccggc     6840

gatctggagc ggcccctgct gggcctgacc tcccgggagt tcgacgggct ggcggaccgc     6900

gtcgacctca tctaccacaa cgcggcccgg gtcagcgccg tcgacaccta cccccggctg     6960

cgggcggcca acgtcggcgg cacccgggag gtgctgcggc tcgcgacccg ttcccgctcc     7020

ggccccgtcc ccgtgcacta catctccacc gccgccgtgt cggccggagc gccgcgcgac     7080

agcggcggcc gggtgcccga ggcccggcgc gtccccctgg acaccctcct gcccggcggc     7140

tacacggcca gcaagtgggc cgccgagcag ctcgtctgga cggcggccga gcggggcgtc     7200

cccgtcaccg tccaccggtg cggacgcatc agcggacaca ccgtcagcgg cgccggacct     7260

gacggcgacg tgctgtggca gctcatccgc gccatgctcg tcaccggcgc cgcgccctgc     7320

ctcaccacgc cgggcgaacc cgtccccgtg gtggatctga tccccgtcga ctacacggcg     7380

tccgccgtcg tccggctctc ccgcaacccg gcgagccagg gcctcgccca ccatctcacc     7440

tgccccgccc cgctcccctt cgactcggtc ctggaccatc tgcggagcta cggctaccgc     7500

ctggagagcc aggagccggg ggagtggacg ctgggtctgc ggcaccgggc cgaccgggcc     7560

ggggaagcgg agcgggagcc cctggacgcg gccctgctgc tcgtcgacac cctgcccgcc     7620

ctcggccggc tgggacgggt cgtcctcgac cgcacccata ccgaggcggg gctcgcgggc     7680

accggcgtcg tcttcccggc cctggacgcc cggctgatcc actcctacgc cgaccacttc     7740

gtcgcctcgg gcttcttccc gcccccggac gcccgctga                            7779


<210>  2
<211>  1233
<212>  DNA
<213>  Streptomyces clavuligerus

<400>  2
gtgaacgacg tgatgcacga ggtgacgcac gacggggcgc acgacgcgag agcaccccgc       60

gcctacccct tctccgaggc ggtcgcgctg gacgtcgacc cgctgtacgc gaaactgcgg      120

gacgaggagc ccgtcccccg ggtgagctgc ccctacggcg aggacgcctg gctggtcacc      180

cggcacgcgg acatgaagac catcctcggc gacccccggt tcagccgggc cgccgccgcg      240

gcccacgacg agtcccggct cacccccctg cccatccaca ccagcatcct gggcatggac      300

ccgcccgacc acacccggct gcgccggctg ctctcccggg tcttcaccat gcgccgggtg      360

gaactcctgc gcccccgcgt cgaacgggag gcccaccggc tcgtcgacgc catcgtcgcc      420

gacggcatgc ccggcgacct catggagggc ttcgccgtcc ccttcgccgg aaccgtcatc      480

tgcgacctcc tcggcgtccc cttcgaggac cgcgagcagt tccgcggctg gctggacgcc      540

ttctccgcca ccaccgtgat gacggaggag gagatcgaac gggacaccga gctgctctac      600

gactacatcg gcaggctggt cgcccgccgc cgggcccggc ccgaggacga cctgctgagc      660

gccatggtca aggccagcga cgaggaggag aggctgtcgg agaaggagct gacggaactc      720

gccagcgtcc tgctcatcgc gggccacgag acggtctcct cgcagctcat cgactccctc      780

catgtgctct tctgccaccc ccgggaactg gccgaactgc tgcggcggcc cgagctgatg      840

ccgcgcgccg tcgaggagct gctccgctat gtgcccctca tctcccatgt caccttcgcc      900

cgctacgcca cggaggacat cgagctgagc gggaccctgg tccgggcggg cgagtccgtc      960

ctgcccgcga tcccctcggc caaccgcgac gaggcggtct tcaccgaccc ggagcgcttc     1020

gaccccaccc gggagcacaa tccgcacctc ggcttcggct acggcatcca ccgctgtctg     1080

ggggcgccgc tggcccggct ggagctccag gtggcgatcg acaccctctt cacccggctg     1140

cccgagctgc gccgcgcggt gccggaggag tccctccagt ggaaggacgg gatgcaggtg     1200

cgcagcctgc tgcggctgcc cgtcctctgg tga                                  1233


<210>  3
<211>  1185
<212>  DNA
<213>  Streptomyces clavuligerus

<400>  3
atgcgtacca ccgaccccgt ccaggaccgt cccgtggacg gcttcagccc cgactcgggc       60

gtcatagccg agatgatcgg gcgcccactg cgcttcgact ccgcgcgcta cgcgccgggc      120

gacagctacg ccgaggtggc ctgcgaccgt gtgctgcgcg cccggcagag cctggggctc      180

accggcgaga ccagggcgtc gatcctggcc ctgctcaccg agttgaccga cccgtggggc      240

aggcgccccg tcggggaacc ccccgcgcgc gcctgctggg tctccatcga cggcatgccc      300

ttcgagccct cggtcgcctg gacggacggg aaggcgaccg tccggatctc cctcgaatcc      360

cccggggacg gcacggccct ctcccggctg cgggacgggc aggccctcac cgaccggctc      420

gacgggcggc ccggggtgga gctgggtccc ctgcgggaga tcgaggacct gttcctctcc      480

gacgaccccc aggggtactt cagcatctcc cacgccgtgg cctggcgcgc ggacggaccc      540

cccgtgtaca agatcttcct caatccggcg gtcgagggac gggagcaggc cgccgcccgg      600

accggtgagg cgctgacccg gctcggcctc ggacggccct ggcaggccct gtccgaccat      660

ctgggcggcg tcttcacccc ggagcacgaa cccgtctgcg tcgccctgga cctgctcggc      720

ggcgacgcgc tgcgggccca ggtctacctc gcgcactccg gggtctccgc cgcggcgatc      780

gacgccaggg cagcggtcgc gcgggaccat gtcccggggc tcttcgcccg cgccctcacc      840

gagatcaacg ggccggacac cgcacccgcg tggggacgca agccccccgt caccaccttc      900

acgttcgacc cccggcacga ccgcccgagc gcggcggcct atatcccgat gatcccggtg      960

cacgaccacg acgccgccgc ccgcgaccgg gcggcggcct tcctccggtc ccggggcgcg     1020

gcggcggccg agccctacac cgccctcctc gacgccctcg cggaccgtcc gctcgacgag     1080

tccctgaccc agaacttcgt ctcctaccgg ggcggggact cccaccgttt ctccgtctat     1140

ctcgccccgg gtgtgtaccg cgcggccgac gcagggaccc ggtga                     1185


<210>  4
<211>  3409
<212>  DNA
<213>  artificial

<220>
<223>  editing template for pKONC_LynC

<400>  4
gaattccttg atcgaggcac accgtatcgc ccaggtctgt gctcctgacg ggctgcgggc       60

ccaccgggcc gagctgatca gccggctcga ccgccagttg ggtccggtgt gaccccgagc      120

ccgtccggcg tggaacgatg cgcgggatcg gcgcgcggtg aggtgcgcgg gacgggaagg      180

ggttctgtgg tgaaggacgg caggggtgtg cggggcgggg aagtggtcgg ggggaacggc      240

cggggaggag gggagcgggt gttgcggttc gggagtgtgg ccgtcgcggg ggccgtgctc      300

acgggtatcg tcgccgtcta catggccctg gtcgcgttcg ggaacatcac cgacttcggg      360

acgaaccggg agttcgtccg tcatgtcctg gccatggaca ccaccttccg ggacgaggac      420

gtgatgtggc gggcgatcga gtcgcacgcc gtggccgatg ccgcgtatgt ggggatcatc      480

gtctgggaga cgctggccgc cgcggtgctg ctcggcgccg tgtggctgtg ggcgcgcggg      540

ctgcggcgcg ccccttccgg aagcggcggg tccgccgggg acatggaccg ggcgcgccgg      600

gtgagcacgc tggggctggt catggtggtg ctgctcttcg ggttcggctt catcgtgatc      660

ggcggggagt ggttccagat gtggcagtcg tccgactgga acggtctgga gcccgcggga      720

cgcaatgtga tgctggcggc gttcaccctg gtggtgatcc atctgcccgg tgcgcggggg      780

agcggggagg acggtcggcc gccgcggtag gcggcgcggt cagggaccgg cccggcggcc      840

ctcacacggg ggagggccgc cgggcctccg cacaggtcac gccgggtggg ccgggcccgg      900

tgtcaggcga gccgccagtc ggggtccacg gggcccgcgg gttcgggggc ggtgcgggtg      960

accaccgggt cgagcaccgc gttcaccgct gccacgaggt cgtcgtcgag acggacgccg     1020

gccgccccgg cgttctccgt gatctgcgcg gggcgcgagg cgccgaggac ggcggtcgac     1080

acctggggct ggtggagcac ccaggccagc gcgagccggg cgaggggcag accggcctcc     1140

gccgccaggg gccgcagccg ttccacccgg ctgaggacct cgtcgttcag ccagcaggac     1200

agcagctcgg acccgtcgtt cacggccgtg gcgcgcgacc ccgcgggcca gggcccgccg     1260

cccggcgggt acttgcccgt caggaccccc tgggccaggg gtgaccaggc gatctgtccc     1320

acgcccagct ccgcgcagac ggatgtcaca ccggtctcgg gcacccgcca cagcgccgag     1380

tactgcacct ggtcggagat cagcggaacg cgcagttccc gtgcgagcgc gtgaccgcgc     1440

cggagctggt cggggctcca ctcgcagacg ccgatgtagt gggccttgcc ggagtggacc     1500

acatcggcca gggcctccat ggtctcctcc agcggggtgg cggggtcgta gcgatgggcc     1560

tggtagacgt ccacgtactc catgcccagc cggcgcaggg agaggtcgat cgactcccgg     1620

atgtgcttgc gggagagtcc gaggtcgttg cggccggggc cggtcggggc gaagaccttg     1680

gtgaggatct ggatccgtat gactgtgacg acggatgacg acgtcctggg cacgggcgat     1740

tccgtcgccg cccgcctgga gaaactgcgg ggctcgcgga tcgtggtgac cggcggcgcc     1800

gggctcgtcg gcagcaggct gaccgccctg ctgcgccgcg ccggggccga ggtgaccgtg     1860

gtggaccgga tggacgccta tccggacggc acccatgagc tgttcggcgc ggcccggacc     1920

gccacccggc tcctggtggg cgacatccgg aaccgcgagc tggtgcggac ggcgctcggc     1980

ggcgccgacc acgtggtcca cgccgccgcc ttcgcggatg tcgccgcctg cacccggcgg     2040

cccgacatcg ccttccgggc caatgtcgag ggcaccgaga cggtcctgga ggaggccgtc     2100

gccgccgagg tgcgccgact ggtcctgatc tcctcggccg cggtctacgg caacggcccc     2160

gccgccgcgg ccggtccgca ccgcttccac gaggaccaga ccacccaccc cctgtccgtc     2220

tacgccaact ccaagctgtg ggccgagcac cagacccggc tgctgctcgg cggcaccggc     2280

accgagtacg cggtcctgcg ctacttctcc gtctacggcg acccgcagat ccccaagccg     2340

ggcagccact cctggatggt ggcctggctc gcgatgcacg cccggctcgg gctgccgatg     2400

cggctcaacg ggggcggggt ccaggtgcgg gacctggtcc atgtcgacga catcgccgcc     2460

gccaccgcgc tcgccctggt cgaggagggt gtcgtcggcg agaccgtcaa cgtcggcacc     2520

ggcatcccca cctcggtgcg cgaggtcggt gaactgatcg ccccgcactt ccccggctcc     2580

cgcttcatca ccaccccccg ccccgcggac gaccccctcg gcggctacgc ggcgaccgag     2640

cggctgcacg aactcctgcg ctggcggccc gcggtctccg tcgcggaggg cgtggaacgc     2700

tatgtgcgct ggctgtcggc cacccccgag gccgtaccgg gctggctggc cgagcgggcc     2760

gcgtgacacc gacggcaggc ccggcacgac cggtcccgtc gccccgggca gccggggcgg     2820

cgggggcggg tcccgacaca gcacacggca cggcacgagg cgcggcacac ggcgtggcac     2880

gagccacggc acagggcacg gcgaaaggca cggcggtacc gatgggatac gccggttcgt     2940

acctctggtc cctgcggcaa gtggtgggca gccgccccct gctcgtcccc ggcgcccagg     3000

tgctcctgat cgaccccgac gggcgggcgc tgttccagcg gcgcgccgac accggggtct     3060

gggaactgcc cgccggggcc tgcgaggagg gcggcgactt cgccgggacc gccgtgcgcg     3120

aactcctgga ggagaccggg ctgcgggtcg aacgggagga cctgatcccc ttcggctgcc     3180

tctccgaccc cgccgtgcac accctgacct accccaacgg cgatgtcgtc cactgcttcg     3240

ccctctgttt cctggcccgc gtctggtccg gcgaactgcg gcccgacccc gaggagtcgg     3300

cggaggcccg cttcctcgac ccggcggccc cgccggaccc gctgcacccg ccgaccgccg     3360

cggtcctgga gatgtaccgc gcgttcacca cgacagagag gttaagctt                 3409


<210>  5
<211>  3667
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  editing template for pKONC_LynO

<400>  5
gaattcgtga tgcggcacgg actcgcgcac cgggccctcg ccgccgtcct ccggcggtac       60

gaggcggcac ccgggcaccc cctcaggggc tcggctcagc tcacggacgc tccgccgcca      120

cggatacgcc ggggccggat accgcccagg aaatcgagcc acgctcccgg ttgatggccg      180

aatgcgcacc attgcgcccg gtgttcctcc cgggattccg gccggccgcc ccgaaggcgc      240

cccgaccgta ggggacttcc ccgggcgtcg tggtgaaccg gctggtcatg gcatcgccct      300

cgggacgtgg caaccgccgg tgcggctgtc cggatgaccg gaccctaccc gggccccggc      360

tcgtcggcca tgtgatttat gtgcccgctc gcgcgcccgc gcgattgtgt atacgaactg      420

ctccggtgca aagaccttcc ggacagattc actgcatcgg gggacctgag ccgccatcca      480

atggcccaac gctcgggcca gcgcctacgg ttctcgcgga tctaggacgg acttcccgga      540

tgccgccctc ttcacctccc aacaccccgg ctgacgtgcg cgtacaccga acgccgatcc      600

gatgaaggag agccctgccc gatgaccgtg gacaccagcc cggagcccca gcaagcgccc      660

cgccagcaga gcagcctcag cacggccgcc gcgcgcaatc tcgccaccac gaccaagtcc      720

gccccgcaga tgcaggagat cacctcccgc tggctgctca ggtcgctccc gtgggtggag      780

gtctccggtg gcacgtaccg ggtgaaccgc cgtctgacct acgccgtcgg tgacggccgt      840

atcgagttcg tccaggaggg cgcccaggtc cgggtgatcc cccacgagct gggtgaactc      900

tcgctgctgc gcggcttcga ggacgaggac gtcctcaccg ccctcgccgg acggtgtgtg      960

cagcgcgact tcaacgccgg tgaagtcctc gtggagcgcg gcaccgccgc cgaccgcatc     1020

catctgatcg cgcacggccg ggtcaaccag atctccgtcg gcgcgtacgg cgacgagacc     1080

gtgaccgccg tcctcggcga cggcgaccgc ttcggccaga gcaccctgct cgaccccgac     1140

gccgcctggg actacaccgc tgtcgccctc agctcgggca ccctgctcac cctctcccgg     1200

gacgacttcg ccgccgtgct ggccggcgcg cctggcctcc agacgcacat cgaggagttc     1260

cgggccctcc cggagcaccg ccagaaccgt ctcggcgagg ccgagatcgc gatgtccgcc     1320

ggccacaccg gcgaggtcga cctgcccggc gccttcgtcg actacgagct gaacccccgt     1380

gagtacgagc tgtccctcgc gcagaccgtg ctgaaggtcc acacgcgcgt ggccgacctc     1440

tacaacaagc cgatgaacca gacggagcag cagctcaggc tcaccgtcga ggcgctacgg     1500

gagcgccagg aatacgagct ggtcaacaac cgcgagttcg gtctgctgca caacgccgcc     1560

ttcaaccagc ggattcagac ccactccggc ccgcccaccc cggacgacct cgacgagctg     1620

ctgtgccgcc gccgcggctc gaagttcttc ttcgcccacc cgcggaccat tgccgcgatc     1680

ggccgggagt tcaacgcccg cggtctctac ccggaccacg tcgacgtcgg cggacagcag     1740

gttcccgcct ggcgcggtgt cccgatcctg ccgtgcaaca agatcccgat caccaaggag     1800

aacaccagcg gatcccgagg tcctcttcgc caccggccgc gccccgcgca ccgaggacat     1860

cggcctctcc accgtgggcc tggagccggg ctcctggctc tccgtcgacg acacctgtcg     1920

ggtcgagggc agcgactggc tctacggcgt cggcgacgtc aaccaccgcg ccctcctcac     1980

ccaccagggc aagtaccagg cccgcatcgc ggcggcggcc atcgtcgccc gcgcccaggg     2040

cgtccccctg ctggagacgg accgctgggg cgcccacagc gccaccgccg accacgccgc     2100

cgtcccccag gtcgtcttca ccgaccccga ggccgccgcc gtcggcctct ccctcgccga     2160

ggccgaagcc gcgggccacc acgtccgcgc cgtcgactac gacctcgcct ccgtcgcggg     2220

cgcctccctc tacctcgacg gctaccgcgg ccgagcccgc atcatcgtcg acctcgaccg     2280

ctccatcctc ctcggcgcca ccttcatcgg ccccggcgtc ggcgaactcc tccactcggc     2340

caccgtcgcg atcgtcggcg agatccccat cgaccgcctc tggcacgcgg tgccctcgta     2400

tccgacgatc agcgaggtgt ggttgcggct gctggagacg tacaggcagg ggtagttgct     2460

ctcagtcctc cgccgccgag gcgacgcggc gatcccttgg ggccggggga accaaggacc     2520

ggccggagct cgttcggcga cgtgtgtggc cgcgccgcgg cgcacgcggt cgagcgcccc     2580

cgccgcatgg gagtgtgccg cgctgtctca ggtgaactgt ttgatctgtc gcagcgtcag     2640

cgccgtctcc acggtgtgga tgccctggag tgtgccgatt cggtcgctga ggcagacgca     2700

gagatcctcg gtggtgcggt ggagggtcgt ggccaccagg ttggcctgcc cggtgacggc     2760

gccggcgaac tgcacctcgg ggtgggtggc gaggtcgttt ccggcggctg cgagggctcc     2820

cggtgccacg gtcagccaca gtatggccgc gacctcgttg ccgagggtct ggcggtcgta     2880

ctggacggtg aagtagagca ctccggtcgt gcgcagttgc tccagccgct gtttcaccac     2940

cgactcggag agcccggtgt ccgtctggag ttcggtgagg gtggcccgtc cgtcgtggtg     3000

cagtgccgcg agcagttgga cggaggcttc gtcgagttcg acgggtccgt tcgcgggttc     3060

gacgggcggc gggcggagcg cggcttcctg ttcggggtcg agggcgtcga tcttgtcgag     3120

ccagccgagg gggccgccgt agaagcggtg gagcaggcac tgggcgctga cggagaccac     3180

ccggcgggtg cgctgcaatc ggctgagcag cagttcgtcc cggtcccgga cggtgcgcgg     3240

ctgcatgacg cacagcacct cggtaccgcc cgagagcagg ccgacccagg cggtgtccgg     3300

gcgccgggcc agggcggcgg ccaggtccgc cgcgccgtcc gaggcgcagc ccaggcgtac     3360

cagccagttg gtgcggccga gccgggtctc gtcggtgatg ccgacgaccc tcagccgcgc     3420

ggtggtgagc agccgccggt agcgccgggc caccgtctgg tcggagacgt cgagcacctg     3480

ggcgatccgg ctgaagggtg cgcgggcgtc gagttgcagc gcctggagca gcttccggtc     3540

gagggggtcc agcacaggtg ctccgcgggg gtcacgcccg cccggacagc ggggcaggaa     3600

ggggggtggg cgggaggccg gggccatcgt aggcggcccc ggccttgcgg acaggcacgg     3660

caagctt                                                               3667


<210>  6
<211>  2917
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  editing template for pKONC_LynS

<400>  6
gaattctcta gaggatcctt cccgtgcgag cgcgtgaccg cgccggagct ggtcggggct       60

ccactcgcag acgccgatgt agtgggcctt gccggagtgg accacatcgg ccagggcctc      120

catggtctcc tccagcgggg tggcggggtc gtagcgatgg gcctggtaga cgtccacgta      180

ctccatgccc agccggcgca gggagaggtc gatcgactcc cggatgtgct tgcgggagag      240

tccgaggtcg ttgcggccgg ggccggtcgg ggcgaagacc ttggtgagga tctccaggcc      300

ctcccggcgt tcgcccttca gcgcccggcc gagggactcc tccgcccggg tgtccgcgta      360

gacgtcggcg gtgtcgaagg tggtgatccc ggcgtcgagc gcggcgtgga cgcaggcgtg      420

ttccgcctcc tccccgcccg ggccgccccg ggtgagccag ttgccgtagg acaaccggct      480

gacgatcagt ccactgcgtc ccagatgacg gaagtccatg gcggtcaccg ggtccctgcg      540

tcggccgcgc ggtacacacc cggggcgaga tagacggaga aacggtggga gtccccgccc      600

cggtaggaga cgaagttctg ggtcagggac tcgtcgagcg gacggtccgc gagggcgtcg      660

aggagggcgg tgtagggctc ggccgccgcc gcgccccggg accggaggaa ggccgccgcc      720

cggtcgcggg cggcggcgtc gtggtcgtgc accgggatca tcgggatata ggccgccgcg      780

ctcgggcggt cgtgccgggg gtcgaacgtg aaggtggtga cggggggctt gcgtccccac      840

gcgggtgcgg tgtccggccc gttgatctcg gtgagggcgc gggcgaagag ccccgggaca      900

tggtcccgcg cgaccgctgc cctggcgtcg atcgccgcgg cggagacccc ggagtgcgcg      960

aggtagacct gggcccgcag cgcgtcgccg ccgagcaggt ccagggcgac gcagacgggt     1020

tcgtgctccg gggtgaagac gccgcccaga tggtcggaca gggcctgcca gggccgtccg     1080

aggccgagcc gggtcagcgc ctcaccggtc cgggcggcgg cctgctcccg tccctcgacc     1140

gccggattga ggaagatctt gtacacgggg ggtccgtccg cgcgccaggc cacggcgtgg     1200

gagatgctga agtacccctg ggggtcgtcg gagaggaaca ggtcctcgat ctcccgcagg     1260

ggacccagct ccaccccggg ccgcccgtcg agccggtcgg tgagggcctg cccgtcccgc     1320

agccgggaga gggccgtgcc gtccccgggg gattcgaggg agatccggac ggtcgccttc     1380

ccgtccgtcc aggcgaccga gggctcgaag ggcatgccgt cgatggagac ccagcaggcg     1440

cgcgcggggg gttccaattg atcctcggcg acccccggtt cagccgggcc gccgccgcgg     1500

cccacgacga gtcccggctc acccccctgc ccatccacac cagcatcctg ggcatggacc     1560

cgcccgacca cacccggctg cgccggctgc tctcccgggt cttcaccatg cgccgggtgg     1620

aactcctgcg cccccgcgtc gaacgggagg cccaccggct cgtcgacgcc atcgtcgccg     1680

acggcatgcc cggcgacctc atggagggct tcgccgtccc cttcgccgga accgtcatct     1740

gcgacctcct cggcgtcccc ttcgaggacc gcgagcagtt ccgcggctgg ctggacgcct     1800

tctccgccac caccgtgatg acggaggagg agatcgaacg ggacaccgag ctgctctacg     1860

actacatcgg caggctggtc gcccgccgcc gggcccggcc cgaggacgac ctgctgagcg     1920

ccatggtcaa ggccagcgac gaggaggaga ggctgtcgga gaaggagctg acggaactcg     1980

ccagcgtcct gctcatcgcg ggccacgaga cggtctcctc gcagctcatc gactccctcc     2040

atgtgctctt ctgccacccc cgggaactgg ccgaactgct gcggcggccc gagctgatgc     2100

cgcgcgccgt cgaggagctg ctccgctatg tgcccctcat ctcccatgtc accttcgccc     2160

gctacgccac ggaggacatc gagctgagcg ggaccctggt ccgggcgggc gagtccgtcc     2220

tgcccgcgat cccctcggcc aaccgcgacg aggcggtctt caccgacccg gagcgcttcg     2280

accccacccg ggagcacaat ccgcacctcg gcttcggcta cggcatccac cgctgtctgg     2340

gggcgccgct ggcccggctg gagctccagg tggcgatcga caccctcttc acccggctgc     2400

ccgagctgcg ccgcgcggtg ccggaggagt ccctccagtg gaaggacggg atgcaggtgc     2460

gcagcctgct gcggctgccc gtcctctggt gaccccgtcc gcgcgggcgg ccggcgccgg     2520

tgaccggagc gggacggcgc gctccggccg gcccggccgc ccgcgcggta cggcgccgga     2580

cggtgcctcc ggtacgggcc cggtctctgg gacactggag acatgaccga tcggaaaccg     2640

ccgggtgtga gcttccagtc ctgggtcgat cagcagatcc acgccgcgga ggcacgggga     2700

gagttcgccg cgctgccggg ggcgggacgc ccgttcgacg aggccatcga ggcgccgtac     2760

gacgaacagt ggtggatcaa gcggaagatg gcccgggagg ggctgagcag cctgccgccc     2820

accctggccc tgcgcaagga ggccgaggac gcgctggagg ccgcgtgggc ggccccgtcg     2880

gcggaggcgg cccggcgtat cgtcgccgcg gaagctt                              2917


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynC 5' flanking fragment

<400>  7
cttgatcgag gcacaccgta tcg                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynC 5' flanking fragment

<400>  8
agatcctcac caaggtcttc gcc                                               23


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynC 3' flanking fragment

<400>  9
gtatgactgt gacgacggat gacg                                              24


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynC 3' flanking fragment

<400>  10
agcgagacgg acgacaggga g                                                 21


<210>  11
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynO 5' flanking fragment

<400>  11
gtgatgcggc acggactcg                                                    19


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynO 5' flanking fragment

<400>  12
gctggtgttc tccttggtga tcg                                               23


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynO 3' flanking fragment

<400>  13
cgaggtcctc ttcgccaccg                                                   20


<210>  14
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynO 3' flanking fragment

<400>  14
gccgtgcctg tccgcaag                                                     18


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynS 5' flanking fragment

<400>  15
ttcccgtgcg agcgcgtgac                                                   20


<210>  16
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lynS 5' flanking fragment

<400>  16
gaaccccccg cgcgc                                                        15


<210>  17
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynS 3' flanking fragment

<400>  17
atcctcggcg acccccg                                                      17


<210>  18
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; lynS 3' flanking fragment

<400>  18
cgcggcgacg atacgccg                                                     18


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleA gene

<400>  19
cggcttcttc gtcaatatgc tggtcc                                            26


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleA gene

<400>  20
gttcacctcg gcgaaggtca gg                                                22


<210>  21
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; parts of tleA and tleB genes and the region between

<400>  21
cttcgactcg gtcctggacc atct                                              24


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; parts of tleA and tleB genes and the region between

<400>  22
agcctgccga tgtagtcgta gagca                                             25


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleB gene upstream region

<400>  23
actgatgaca actgatgaca ttcggaca                                          28


<210>  24
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleB gene upstream region

<400>  24
gagaagtagc gcaggaccgc gtact                                             25


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; inside of 3' 138kb region joint

<400>  25
cgtctcgtac gtcctgaaca acac                                              24


<210>  26
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; inside of 3' 138kb region joint

<400>  26
gtagtcgtcg ttgtacgcgt acac                                              24


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; across the 3' 138kb region joint

<400>  27
gagtcggaac accacgtcct gacc                                              24


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; across the 3' 138kb region joint

<400>  28
gctgatcgtc ggatacgagg gca                                               23


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleC gene

<400>  29
ggctccactc gcagacgcc                                                    19


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleC gene

<400>  30
gggtctccat cgacggcatg                                                   20


<210>  31
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleB gene

<400>  31
gacatgaaga ccatcctcgg cg                                                22


<210>  32
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer; tleB gene

<400>  32
ggcggcaggc tgctcagc                                                     18


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer codA_XbaI_R

<400>  33
gcgctctaga gcgcttgtag tcgatggcct                                        30


<210>  34
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer codA_NdeI_F

<400>  34
gcggcatatg gttgacatct tttgccgatt ctgg                                   34


<210>  35
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Apr (aac3)_XbaI_F

<400>  35
cgcgtctaga ggatcttcac ctagatcctt ttggttc                                37


<210>  36
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer ApR(aac3)_EcoRI

<400>  36
ccgggaattc cccgatccgc tccacgtg                                          28


<210>  37
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_L_F_EcoRI

<400>  37
gcgcgaattc cttgatcgag gcacaccgta tcg                                    33


<210>  38
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_L_R_BamHI

<400>  38
gcgcggatcc agatcctcac caaggtcttc gcc                                    33


<210>  39
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_R_F_BamHI

<400>  39
gcgcggatcc gtatgactgt gacgacggat gacg                                   34


<210>  40
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_R_R_HindIII

<400>  40
gcgcaagctt agcgagacgg acgacaggga g                                      31


<210>  41
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_L_F_EcoRI

<400>  41
gcgcgaattc gtgatgcggc acggactcg                                         29


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_L_R_BamHI

<400>  42
gcgcggatcc gctggtgttc tccttggtga tcg                                    33


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_R_F_BamHI

<400>  43
gcgcggatcc cgaggtcctc ttcgccaccg                                        30


<210>  44
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_R_R_HindIII

<400>  44
gcgcaagctt gccgtgcctg tccgcaag                                          28


<210>  45
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_confF

<400>  45
acctctacaa caagccgatg aaccaga                                           27


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_confR

<400>  46
gctgatcgtc ggatacgagg gca                                               23


<210>  47
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynO_IN_confF

<400>  47
gagtcggaac accacgtcct gacc                                              24


<210>  48
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_confF

<400>  48
gtactgcacc tggtcggaga tcagc                                             25


<210>  49
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_confR

<400>  49
gagaagtagc gcaggaccgc gtact                                             25


<210>  50
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynC_IN_confF

<400>  50
actgatgaca actgatgaca ttcggaca                                          28


<210>  51
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynS_confF

<400>  51
taggagacga agttctgggt caggga                                            26


<210>  52
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynS_confR

<400>  52
agcctgccga tgtagtcgta gagca                                             25


<210>  53
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer lynS_IN_confF

<400>  53
cttcgactcg gtcctggacc atct                                              24


<210>  54
<211>  52
<212>  DNA
<213>  Streptomyces clavuligerus

<400>  54
gtcgacgcgg tcgggcgtgg cgagggcggg atcggcgtag agggagcgtt cc               52


<210>  55
<211>  43
<212>  DNA
<213>  Streptomyces clavuligerus

<400>  55
gtgctgatcg aacggcgcag cgcgagcccg atgctcaccc ccg                         43


