               SEQUENCE LISTING

<110> Rijk Zwaan Zaadteelt en Zaadhandel B.V.

<120> Mutant Cullin1 gene in watermelon

<130> P170424PC01

<140> 
<141> 2021-07-15

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 2232
<212> DNA
<213> Citrullus lanatus


<220> 
<223> Wild type cullin1 gene

<400> 1
atgacaatgg gcgagcggaa gactattgac ttggaacaag gatgggagtt tatgcagaag      60

ggaatcacaa agttgaagaa cattcttgag ggcttgcctg agcctcagtt cagctccgag     120

gactacatga tgctttatac caccatatac aacatgtgca cacaaaagcc gccacatgat     180

tactcccagc agctatacga taaataccgt gaatcttttg aggagtatat cacttctatg     240

gtcttaccat ccttgaggga gaagcatgac gagttcatgt tgagagaact ggtcaaaagg     300

tggacgaacc ataaagtcat ggtgaggtgg ctttctcgct tcttccacta tcttgaccga     360

tacttcattg ctcgaagatc acttccacct ctcaacgaag ttggcctcac atgcttccgt     420

gaattggtgt acaaagagct aaacagtaaa gtgagggatg cagtaatttc attgattgat     480

caagaacgtg aaggagagca gattgacaga gctctactga agaatgtatt agatatattt     540

gtggaaattg ggatggggca aatggattac tatgaaaatg actttgaagc tgccatgctt     600

aaagatactg ctgcttatta ctctaggaag gcttccaatt ggatcctaga agattcttgt     660

cccgattata tgctaaaagc agaggagtgc ttgaaacgag aaaaggatag agtttctcac     720

tatttgcact ctagtagcga gccaaagtta ttagagaaag ttcaacatga actgttatct     780

gtgtatgcta ctcaactgct ggaaaaagag cattcaggat gccatgcatt gcttagagat     840

gacaaggtgg aagatttgtc aaggatgttc cgcctcttct ccaaaatacc caagggattg     900

gacccagttt ccaacatatt taagcagcat gtcactgctg aaggaacagc attggtcaaa     960

caggcagaag atgctgcaag taacaagaag gccgagaaaa aggacatagt tggtctgcag    1020

gaacaggttt ttgtaagaaa agtgattgag cttcacgaca agtacttggc ttacgtgaat    1080

gattgtttcc aaaaccacac actttttcac aaggctctca aggaagcttt tgaagtcttt    1140

tgcaataagg gtgttgctgg aagttctagt gcagaattac ttgctacctt ttgtgataac    1200

atccttaaga aaggtgggag cgagaagttg agtgatgaag caattgagga gacacttgag    1260

aaggtcgtga agttgctggc atacatctgc gacaaagatc tgtttgctga attctataga    1320

aaaaaacttg cccgaaggct tctctttgac aagagtgcca acgatgacca tgagagaagt    1380

atattgacca aattgaagca acaatgtggt ggccagttca cctctaagat ggaggggatg    1440

gtcactgatt tgactttggc aagggagaac caaactagtt tcgaggagta tctgagcaat    1500

aatccacaag ctagtcctgg aatcgacttg actgtcactg ttttgactac tggcttttgg    1560

ccaagctaca agtcttttga cctcaacctg ccggcagaga tggtaaagtg tgttgaagtt    1620

ttcagagagt tctatcaaac aaaaacaaag catagaaaac ttacatggat ttactcattg    1680

ggtacctgta acatcagcgg aaaatttgaa ccgaaaacga tggagctgat tgtaacaact    1740

tatcaggctt ctgccctgct gctattcaat tcctcagata gattaagtta ttccgagatc    1800

atgacacaat taaatttgag tgacgatgat gttgttagac tgctccactc attgtcatgt    1860

gccaagtata aaattcttaa taaggagccg aacacgaaaa ccatctctcc gaatgatcat    1920

tttgagttca atgcaaaatt ctccgacaaa atgaggagaa taaagatccc tcttccgcct    1980

gtggatgaga aaaagaaagt cattgaagat gttgacaagg atcgaaggta tgctattgat    2040

gcctcaatcg tgcgtatcat gaagagtcgg aaagttctgg gtcatcagca gctagtgatg    2100

gagtgcgtcg agcaattggg tcgtatgttc aagagagaga caaagacaac ccccacttgt    2160

ttaggtactt ggcttgatct tgccttaacc atgggcaaga gtggtggtct ggtgctattt    2220

gatatagcat ga                                                        2232


<210> 2
<211> 743
<212> PRT
<213> Citrullus lanatus


<220> 
<223> Wild type cullin1 protein

<400> 2
Met Thr Met Gly Glu Arg Lys Thr Ile Asp Leu Glu Gln Gly Trp Glu 
1               5                   10                  15      
Phe Met Gln Lys Gly Ile Thr Lys Leu Lys Asn Ile Leu Glu Gly Leu 
            20                  25                  30          
Pro Glu Pro Gln Phe Ser Ser Glu Asp Tyr Met Met Leu Tyr Thr Thr 
        35                  40                  45              
Ile Tyr Asn Met Cys Thr Gln Lys Pro Pro His Asp Tyr Ser Gln Gln 
    50                  55                  60                  
Leu Tyr Asp Lys Tyr Arg Glu Ser Phe Glu Glu Tyr Ile Thr Ser Met 
65                  70                  75                  80  
Val Leu Pro Ser Leu Arg Glu Lys His Asp Glu Phe Met Leu Arg Glu 
                85                  90                  95      
Leu Val Lys Arg Trp Thr Asn His Lys Val Met Val Arg Trp Leu Ser 
            100                 105                 110         
Arg Phe Phe His Tyr Leu Asp Arg Tyr Phe Ile Ala Arg Arg Ser Leu 
        115                 120                 125             
Pro Pro Leu Asn Glu Val Gly Leu Thr Cys Phe Arg Glu Leu Val Tyr 
    130                 135                 140                 
Lys Glu Leu Asn Ser Lys Val Arg Asp Ala Val Ile Ser Leu Ile Asp 
145                 150                 155                 160 
Gln Glu Arg Glu Gly Glu Gln Ile Asp Arg Ala Leu Leu Lys Asn Val 
                165                 170                 175     
Leu Asp Ile Phe Val Glu Ile Gly Met Gly Gln Met Asp Tyr Tyr Glu 
            180                 185                 190         
Asn Asp Phe Glu Ala Ala Met Leu Lys Asp Thr Ala Ala Tyr Tyr Ser 
        195                 200                 205             
Arg Lys Ala Ser Asn Trp Ile Leu Glu Asp Ser Cys Pro Asp Tyr Met 
    210                 215                 220                 
Leu Lys Ala Glu Glu Cys Leu Lys Arg Glu Lys Asp Arg Val Ser His 
225                 230                 235                 240 
Tyr Leu His Ser Ser Ser Glu Pro Lys Leu Leu Glu Lys Val Gln His 
                245                 250                 255     
Glu Leu Leu Ser Val Tyr Ala Thr Gln Leu Leu Glu Lys Glu His Ser 
            260                 265                 270         
Gly Cys His Ala Leu Leu Arg Asp Asp Lys Val Glu Asp Leu Ser Arg 
        275                 280                 285             
Met Phe Arg Leu Phe Ser Lys Ile Pro Lys Gly Leu Asp Pro Val Ser 
    290                 295                 300                 
Asn Ile Phe Lys Gln His Val Thr Ala Glu Gly Thr Ala Leu Val Lys 
305                 310                 315                 320 
Gln Ala Glu Asp Ala Ala Ser Asn Lys Lys Ala Glu Lys Lys Asp Ile 
                325                 330                 335     
Val Gly Leu Gln Glu Gln Val Phe Val Arg Lys Val Ile Glu Leu His 
            340                 345                 350         
Asp Lys Tyr Leu Ala Tyr Val Asn Asp Cys Phe Gln Asn His Thr Leu 
        355                 360                 365             
Phe His Lys Ala Leu Lys Glu Ala Phe Glu Val Phe Cys Asn Lys Gly 
    370                 375                 380                 
Val Ala Gly Ser Ser Ser Ala Glu Leu Leu Ala Thr Phe Cys Asp Asn 
385                 390                 395                 400 
Ile Leu Lys Lys Gly Gly Ser Glu Lys Leu Ser Asp Glu Ala Ile Glu 
                405                 410                 415     
Glu Thr Leu Glu Lys Val Val Lys Leu Leu Ala Tyr Ile Cys Asp Lys 
            420                 425                 430         
Asp Leu Phe Ala Glu Phe Tyr Arg Lys Lys Leu Ala Arg Arg Leu Leu 
        435                 440                 445             
Phe Asp Lys Ser Ala Asn Asp Asp His Glu Arg Ser Ile Leu Thr Lys 
    450                 455                 460                 
Leu Lys Gln Gln Cys Gly Gly Gln Phe Thr Ser Lys Met Glu Gly Met 
465                 470                 475                 480 
Val Thr Asp Leu Thr Leu Ala Arg Glu Asn Gln Thr Ser Phe Glu Glu 
                485                 490                 495     
Tyr Leu Ser Asn Asn Pro Gln Ala Ser Pro Gly Ile Asp Leu Thr Val 
            500                 505                 510         
Thr Val Leu Thr Thr Gly Phe Trp Pro Ser Tyr Lys Ser Phe Asp Leu 
        515                 520                 525             
Asn Leu Pro Ala Glu Met Val Lys Cys Val Glu Val Phe Arg Glu Phe 
    530                 535                 540                 
Tyr Gln Thr Lys Thr Lys His Arg Lys Leu Thr Trp Ile Tyr Ser Leu 
545                 550                 555                 560 
Gly Thr Cys Asn Ile Ser Gly Lys Phe Glu Pro Lys Thr Met Glu Leu 
                565                 570                 575     
Ile Val Thr Thr Tyr Gln Ala Ser Ala Leu Leu Leu Phe Asn Ser Ser 
            580                 585                 590         
Asp Arg Leu Ser Tyr Ser Glu Ile Met Thr Gln Leu Asn Leu Ser Asp 
        595                 600                 605             
Asp Asp Val Val Arg Leu Leu His Ser Leu Ser Cys Ala Lys Tyr Lys 
    610                 615                 620                 
Ile Leu Asn Lys Glu Pro Asn Thr Lys Thr Ile Ser Pro Asn Asp His 
625                 630                 635                 640 
Phe Glu Phe Asn Ala Lys Phe Ser Asp Lys Met Arg Arg Ile Lys Ile 
                645                 650                 655     
Pro Leu Pro Pro Val Asp Glu Lys Lys Lys Val Ile Glu Asp Val Asp 
            660                 665                 670         
Lys Asp Arg Arg Tyr Ala Ile Asp Ala Ser Ile Val Arg Ile Met Lys 
        675                 680                 685             
Ser Arg Lys Val Leu Gly His Gln Gln Leu Val Met Glu Cys Val Glu 
    690                 695                 700                 
Gln Leu Gly Arg Met Phe Lys Arg Glu Thr Lys Thr Thr Pro Thr Cys 
705                 710                 715                 720 
Leu Gly Thr Trp Leu Asp Leu Ala Leu Thr Met Gly Lys Ser Gly Gly 
                725                 730                 735     
Leu Val Leu Phe Asp Ile Ala 
            740             

<210> 3
<211> 2232
<212> DNA
<213> Citrullus lanatus


<220> 
<223> Mutant Cullin1 gene

<400> 3
atgacaatgg gcgagcggaa gactattgac ttggaacaag gatgggagtt tatgcagaag      60

ggaatcacaa agttgaagaa cattcttgag ggcttgcctg agcctcagtt cagctccgag     120

gactacatga tgctttatac caccatatac aacatgtgca cacaaaagcc gccacatgat     180

tactcccagc agctatacga taaataccgt gaatcttttg aggagtatat cacttctatg     240

gtcttaccat ccttgaggga gaagcatgac gagttcatgt tgagagaact ggtcaaaagg     300

tggacgaacc ataaagtcat ggtgaggtgg ctttctcgct tcttccacta tcttgaccga     360

tacttcattg ctcgaagatc acttccacct ctcaacgaag ttggcctcac atgcttccgt     420

gaattggtgt acaaagagct aaacagtaaa gtgagggatg cagtaatttc attgattgat     480

caagaacgtg aaggagagca gattgacaga gctctactga agaatgtatt agatatattt     540

gtggaaattg ggatggggca aatggattac tatgaaaatg actttgaagc tgccatgctt     600

aaagatactg ctgcttatta ctctaggaag gcttccaatt ggatcctaga agattcttgt     660

cccgattata tgctaaaagc agaggagtgc ttgaaacgag aaaaggatag agtttctcac     720

tatttgcact ctagtagcga gccaaagtta ttagagaaag ttcaacatga actgttatct     780

gtgtatgcta ctcaactgct ggaaaaagag cattcaggat gccatgcatt gcttagagat     840

gacaaggtgg aagatttgtc aaggatgttc cgcctcttct ccaaaatacc caagggattg     900

gacccagttt ccaacatatt taagcagcat gtcactgctg aaggaacagc attggtcaaa     960

caggcagaag atgctgcaag taacaagaag gccgagaaaa aggacatagt tggtctgcag    1020

gaacaggttt ttgtaagaaa agtgattgag cttcacgaca agtacttggc ttacgtgaat    1080

gattgtttcc aaaaccacac actttttcac aaggctctca aggaagcttt tgaagtcttt    1140

tgcaataagg gtgttgctgg aagttctagt gcagaattac ttgctacctt ttgtgataac    1200

atccttaaga aaggtgggag cgagaagttg agtgatgaag caattgagga gacacttgag    1260

aaggtcgtga agttgctggc atacatctgc gacaaagatc tgtttgctga attctataga    1320

aaaaaacttg cccgaaggct tctctttgac aagagtgcca acgatgacca tgagagaagt    1380

atattgacca aattgaagca acaatgtggt ggccagttca cctctaagat ggaggggatg    1440

gtcactgatt tgactttggc aagggagaac caaactagtt tcgaggagta tctgagcaat    1500

aatccacaag ctagtcctgg aatcgacttg actgtcactg ttttgactac tgacttttgg    1560

ccaagctaca agtcttttga cctcaacctg ccggcagaga tggtaaagtg tgttgaagtt    1620

ttcagagagt tctatcaaac aaaaacaaag catagaaaac ttacatggat ttactcattg    1680

ggtacctgta acatcagcgg aaaatttgaa ccgaaaacga tggagctgat tgtaacaact    1740

tatcaggctt ctgccctgct gctattcaat tcctcagata gattaagtta ttccgagatc    1800

atgacacaat taaatttgag tgacgatgat gttgttagac tgctccactc attgtcatgt    1860

gccaagtata aaattcttaa taaggagccg aacacgaaaa ccatctctcc gaatgatcat    1920

tttgagttca atgcaaaatt ctccgacaaa atgaggagaa taaagatccc tcttccgcct    1980

gtggatgaga aaaagaaagt cattgaagat gttgacaagg atcgaaggta tgctattgat    2040

gcctcaatcg tgcgtatcat gaagagtcgg aaagttctgg gtcatcagca gctagtgatg    2100

gagtgcgtcg agcaattggg tcgtatgttc aagagagaga caaagacaac ccccacttgt    2160

ttaggtactt ggcttgatct tgccttaacc atgggcaaga gtggtggtct ggtgctattt    2220

gatatagcat ga                                                        2232


<210> 4
<211> 743
<212> PRT
<213> Citrullus lanatus


<220> 
<223> Mutant cullin1 protein

<400> 4
Met Thr Met Gly Glu Arg Lys Thr Ile Asp Leu Glu Gln Gly Trp Glu 
1               5                   10                  15      
Phe Met Gln Lys Gly Ile Thr Lys Leu Lys Asn Ile Leu Glu Gly Leu 
            20                  25                  30          
Pro Glu Pro Gln Phe Ser Ser Glu Asp Tyr Met Met Leu Tyr Thr Thr 
        35                  40                  45              
Ile Tyr Asn Met Cys Thr Gln Lys Pro Pro His Asp Tyr Ser Gln Gln 
    50                  55                  60                  
Leu Tyr Asp Lys Tyr Arg Glu Ser Phe Glu Glu Tyr Ile Thr Ser Met 
65                  70                  75                  80  
Val Leu Pro Ser Leu Arg Glu Lys His Asp Glu Phe Met Leu Arg Glu 
                85                  90                  95      
Leu Val Lys Arg Trp Thr Asn His Lys Val Met Val Arg Trp Leu Ser 
            100                 105                 110         
Arg Phe Phe His Tyr Leu Asp Arg Tyr Phe Ile Ala Arg Arg Ser Leu 
        115                 120                 125             
Pro Pro Leu Asn Glu Val Gly Leu Thr Cys Phe Arg Glu Leu Val Tyr 
    130                 135                 140                 
Lys Glu Leu Asn Ser Lys Val Arg Asp Ala Val Ile Ser Leu Ile Asp 
145                 150                 155                 160 
Gln Glu Arg Glu Gly Glu Gln Ile Asp Arg Ala Leu Leu Lys Asn Val 
                165                 170                 175     
Leu Asp Ile Phe Val Glu Ile Gly Met Gly Gln Met Asp Tyr Tyr Glu 
            180                 185                 190         
Asn Asp Phe Glu Ala Ala Met Leu Lys Asp Thr Ala Ala Tyr Tyr Ser 
        195                 200                 205             
Arg Lys Ala Ser Asn Trp Ile Leu Glu Asp Ser Cys Pro Asp Tyr Met 
    210                 215                 220                 
Leu Lys Ala Glu Glu Cys Leu Lys Arg Glu Lys Asp Arg Val Ser His 
225                 230                 235                 240 
Tyr Leu His Ser Ser Ser Glu Pro Lys Leu Leu Glu Lys Val Gln His 
                245                 250                 255     
Glu Leu Leu Ser Val Tyr Ala Thr Gln Leu Leu Glu Lys Glu His Ser 
            260                 265                 270         
Gly Cys His Ala Leu Leu Arg Asp Asp Lys Val Glu Asp Leu Ser Arg 
        275                 280                 285             
Met Phe Arg Leu Phe Ser Lys Ile Pro Lys Gly Leu Asp Pro Val Ser 
    290                 295                 300                 
Asn Ile Phe Lys Gln His Val Thr Ala Glu Gly Thr Ala Leu Val Lys 
305                 310                 315                 320 
Gln Ala Glu Asp Ala Ala Ser Asn Lys Lys Ala Glu Lys Lys Asp Ile 
                325                 330                 335     
Val Gly Leu Gln Glu Gln Val Phe Val Arg Lys Val Ile Glu Leu His 
            340                 345                 350         
Asp Lys Tyr Leu Ala Tyr Val Asn Asp Cys Phe Gln Asn His Thr Leu 
        355                 360                 365             
Phe His Lys Ala Leu Lys Glu Ala Phe Glu Val Phe Cys Asn Lys Gly 
    370                 375                 380                 
Val Ala Gly Ser Ser Ser Ala Glu Leu Leu Ala Thr Phe Cys Asp Asn 
385                 390                 395                 400 
Ile Leu Lys Lys Gly Gly Ser Glu Lys Leu Ser Asp Glu Ala Ile Glu 
                405                 410                 415     
Glu Thr Leu Glu Lys Val Val Lys Leu Leu Ala Tyr Ile Cys Asp Lys 
            420                 425                 430         
Asp Leu Phe Ala Glu Phe Tyr Arg Lys Lys Leu Ala Arg Arg Leu Leu 
        435                 440                 445             
Phe Asp Lys Ser Ala Asn Asp Asp His Glu Arg Ser Ile Leu Thr Lys 
    450                 455                 460                 
Leu Lys Gln Gln Cys Gly Gly Gln Phe Thr Ser Lys Met Glu Gly Met 
465                 470                 475                 480 
Val Thr Asp Leu Thr Leu Ala Arg Glu Asn Gln Thr Ser Phe Glu Glu 
                485                 490                 495     
Tyr Leu Ser Asn Asn Pro Gln Ala Ser Pro Gly Ile Asp Leu Thr Val 
            500                 505                 510         
Thr Val Leu Thr Thr Asp Phe Trp Pro Ser Tyr Lys Ser Phe Asp Leu 
        515                 520                 525             
Asn Leu Pro Ala Glu Met Val Lys Cys Val Glu Val Phe Arg Glu Phe 
    530                 535                 540                 
Tyr Gln Thr Lys Thr Lys His Arg Lys Leu Thr Trp Ile Tyr Ser Leu 
545                 550                 555                 560 
Gly Thr Cys Asn Ile Ser Gly Lys Phe Glu Pro Lys Thr Met Glu Leu 
                565                 570                 575     
Ile Val Thr Thr Tyr Gln Ala Ser Ala Leu Leu Leu Phe Asn Ser Ser 
            580                 585                 590         
Asp Arg Leu Ser Tyr Ser Glu Ile Met Thr Gln Leu Asn Leu Ser Asp 
        595                 600                 605             
Asp Asp Val Val Arg Leu Leu His Ser Leu Ser Cys Ala Lys Tyr Lys 
    610                 615                 620                 
Ile Leu Asn Lys Glu Pro Asn Thr Lys Thr Ile Ser Pro Asn Asp His 
625                 630                 635                 640 
Phe Glu Phe Asn Ala Lys Phe Ser Asp Lys Met Arg Arg Ile Lys Ile 
                645                 650                 655     
Pro Leu Pro Pro Val Asp Glu Lys Lys Lys Val Ile Glu Asp Val Asp 
            660                 665                 670         
Lys Asp Arg Arg Tyr Ala Ile Asp Ala Ser Ile Val Arg Ile Met Lys 
        675                 680                 685             
Ser Arg Lys Val Leu Gly His Gln Gln Leu Val Met Glu Cys Val Glu 
    690                 695                 700                 
Gln Leu Gly Arg Met Phe Lys Arg Glu Thr Lys Thr Thr Pro Thr Cys 
705                 710                 715                 720 
Leu Gly Thr Trp Leu Asp Leu Ala Leu Thr Met Gly Lys Ser Gly Gly 
                725                 730                 735     
Leu Val Leu Phe Asp Ile Ala 
            740             

<210> 5
<211> 63
<212> DNA
<213> Citrullus lanatus


<220> 
<223> Marker Wildtype A

<400> 5
gactgtcact gttttgacta ctggcttttg gccaagctac aagtcttttg acctcaacct     60

gcc                                                                   63


<210> 6
<211> 63
<212> DNA
<213> Citrullus lanatus


<220> 
<223> Marker mutant B

<400> 6
gactgtcact gttttgacta ctgacttttg gccaagctac aagtcttttg acctcaacct     60

gcc                                                                   63


