                         SEQUENCE LISTING

<110>  Academia Sinica
       The Regents of the University of California
 
<120>  RECOMBINANT MICROORGANISM

<130>  00159-002WO1

<140>  Not yet assigned
<141>  2021-07-14

<150>  US 63/051,672
<151>  2020-07-14

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  633
<212>  DNA
<213>  Bacillus subtilis


<220>
<221>  CDS
<222>  (1)..(633)

<400>  1
atg gaa tta cag ctt gca tta gac ctc gtc aac atc cca gaa gcc att         48
Met Glu Leu Gln Leu Ala Leu Asp Leu Val Asn Ile Pro Glu Ala Ile           
1               5                   10                  15                

gag ctc gtc aaa gag gta gaa caa tac atc gac gta gtt gaa atc gga         96
Glu Leu Val Lys Glu Val Glu Gln Tyr Ile Asp Val Val Glu Ile Gly           
            20                  25                  30                    

aca ccg gtc gtc att aat gaa ggc cta aga gcc gtt aaa gaa tta aaa        144
Thr Pro Val Val Ile Asn Glu Gly Leu Arg Ala Val Lys Glu Leu Lys           
        35                  40                  45                        

gaa gca ttt cct caa ttg aag gtt ctt gca gac ctg aaa atc atg gat        192
Glu Ala Phe Pro Gln Leu Lys Val Leu Ala Asp Leu Lys Ile Met Asp           
    50                  55                  60                            

gcc gga ggc tac gaa att atg aaa gcg tcg gaa gca ggc gct gac atc        240
Ala Gly Gly Tyr Glu Ile Met Lys Ala Ser Glu Ala Gly Ala Asp Ile           
65                  70                  75                  80            

atc acc gtt tta ggg gct aca gac gac gca acg att aaa ggc gca gta        288
Ile Thr Val Leu Gly Ala Thr Asp Asp Ala Thr Ile Lys Gly Ala Val           
                85                  90                  95                

gaa gaa gcc aaa aaa caa aag aag aaa atc tta gtg gac atg att aac        336
Glu Glu Ala Lys Lys Gln Lys Lys Lys Ile Leu Val Asp Met Ile Asn           
            100                 105                 110                   

gtg aaa gat atc gag tcc cgt gcg caa gaa att gac gca ctc ggt gtt        384
Val Lys Asp Ile Glu Ser Arg Ala Gln Glu Ile Asp Ala Leu Gly Val           
        115                 120                 125                       

gac tac atc tgc gtc cac act ggc tat gat ctt caa gca gag ggc aag        432
Asp Tyr Ile Cys Val His Thr Gly Tyr Asp Leu Gln Ala Glu Gly Lys           
    130                 135                 140                           

aac tct ttc gaa gaa tta acg aca atc aaa aac acc gta aaa aac gca        480
Asn Ser Phe Glu Glu Leu Thr Thr Ile Lys Asn Thr Val Lys Asn Ala           
145                 150                 155                 160           

aaa acc gca atc gcg ggc ggc atc aaa ctt gat aca ctg cca gaa gtg        528
Lys Thr Ala Ile Ala Gly Gly Ile Lys Leu Asp Thr Leu Pro Glu Val           
                165                 170                 175               

atc aag caa aac ccc gac ctt gtc att gtt ggg ggc gga att aca agc        576
Ile Lys Gln Asn Pro Asp Leu Val Ile Val Gly Gly Gly Ile Thr Ser           
            180                 185                 190                   

gca gct gat aag gca gaa aca gct tca aaa atg aag cag ctg att gtc        624
Ala Ala Asp Lys Ala Glu Thr Ala Ser Lys Met Lys Gln Leu Ile Val           
        195                 200                 205                       

caa gga taa                                                            633
Gln Gly                                                                   
    210                                                                   


<210>  2
<211>  210
<212>  PRT
<213>  Bacillus subtilis

<400>  2

Met Glu Leu Gln Leu Ala Leu Asp Leu Val Asn Ile Pro Glu Ala Ile 
1               5                   10                  15      


Glu Leu Val Lys Glu Val Glu Gln Tyr Ile Asp Val Val Glu Ile Gly 
            20                  25                  30          


Thr Pro Val Val Ile Asn Glu Gly Leu Arg Ala Val Lys Glu Leu Lys 
        35                  40                  45              


Glu Ala Phe Pro Gln Leu Lys Val Leu Ala Asp Leu Lys Ile Met Asp 
    50                  55                  60                  


Ala Gly Gly Tyr Glu Ile Met Lys Ala Ser Glu Ala Gly Ala Asp Ile 
65                  70                  75                  80  


Ile Thr Val Leu Gly Ala Thr Asp Asp Ala Thr Ile Lys Gly Ala Val 
                85                  90                  95      


Glu Glu Ala Lys Lys Gln Lys Lys Lys Ile Leu Val Asp Met Ile Asn 
            100                 105                 110         


Val Lys Asp Ile Glu Ser Arg Ala Gln Glu Ile Asp Ala Leu Gly Val 
        115                 120                 125             


Asp Tyr Ile Cys Val His Thr Gly Tyr Asp Leu Gln Ala Glu Gly Lys 
    130                 135                 140                 


Asn Ser Phe Glu Glu Leu Thr Thr Ile Lys Asn Thr Val Lys Asn Ala 
145                 150                 155                 160 


Lys Thr Ala Ile Ala Gly Gly Ile Lys Leu Asp Thr Leu Pro Glu Val 
                165                 170                 175     


Ile Lys Gln Asn Pro Asp Leu Val Ile Val Gly Gly Gly Ile Thr Ser 
            180                 185                 190         


Ala Ala Asp Lys Ala Glu Thr Ala Ser Lys Met Lys Gln Leu Ile Val 
        195                 200                 205             


Gln Gly 
    210 


<210>  3
<211>  687
<212>  DNA
<213>  Methylobacillus flagellatum


<220>
<221>  CDS
<222>  (1)..(687)

<400>  3
gtg gca aaa cca tta gtt caa atg gca tta gat tca cta gat ttc gat         48
Val Ala Lys Pro Leu Val Gln Met Ala Leu Asp Ser Leu Asp Phe Asp           
1               5                   10                  15                

cag act gta gcg ctt gct acg act gtt gca cca cat gtt gat att ctt         96
Gln Thr Val Ala Leu Ala Thr Thr Val Ala Pro His Val Asp Ile Leu           
            20                  25                  30                    

gaa atc ggt act cct tgt atc aag tac aac ggt atc aag ttg ctg gag        144
Glu Ile Gly Thr Pro Cys Ile Lys Tyr Asn Gly Ile Lys Leu Leu Glu           
        35                  40                  45                        

act ctc cgc gca aag ttc cct aac aac aag atc ctg gtt gac ctg aag        192
Thr Leu Arg Ala Lys Phe Pro Asn Asn Lys Ile Leu Val Asp Leu Lys           
    50                  55                  60                            

acc atg gat gct ggt ttt tac gaa gca gag cct ttc tac aag gca ggt        240
Thr Met Asp Ala Gly Phe Tyr Glu Ala Glu Pro Phe Tyr Lys Ala Gly           
65                  70                  75                  80            

gcc gac atc gtg acc gtg ctc ggc act gct gac att ggc acg atc aaa        288
Ala Asp Ile Val Thr Val Leu Gly Thr Ala Asp Ile Gly Thr Ile Lys           
                85                  90                  95                

ggc gtc att gat gtt gcc aac aaa tac ggc aag aag gct caa gtc gac        336
Gly Val Ile Asp Val Ala Asn Lys Tyr Gly Lys Lys Ala Gln Val Asp           
            100                 105                 110                   

ctg atc aac gtg act gac aag gct gca cgc acc aag gaa gtg gcc aag        384
Leu Ile Asn Val Thr Asp Lys Ala Ala Arg Thr Lys Glu Val Ala Lys           
        115                 120                 125                       

ctc ggc gct cac atc att ggc gtt cac act ggt ttg gat caa cag gct        432
Leu Gly Ala His Ile Ile Gly Val His Thr Gly Leu Asp Gln Gln Ala           
    130                 135                 140                           

gct ggt cag aca ccg ttt gcc gat ctc aac ctt gtt tcc agc ctg aac        480
Ala Gly Gln Thr Pro Phe Ala Asp Leu Asn Leu Val Ser Ser Leu Asn           
145                 150                 155                 160           

ctg ggt gtt gac att tcc gta gct ggt ggc gtg aag gcg act acc gcc        528
Leu Gly Val Asp Ile Ser Val Ala Gly Gly Val Lys Ala Thr Thr Ala           
                165                 170                 175               

aaa caa gtg gtt gat gca ggt gcc aca att gtt gtt gct ggt gcg gct        576
Lys Gln Val Val Asp Ala Gly Ala Thr Ile Val Val Ala Gly Ala Ala           
            180                 185                 190                   

atc tat ggt gct gcc gat cct gct gct gct gct gct gaa atc agc gct        624
Ile Tyr Gly Ala Ala Asp Pro Ala Ala Ala Ala Ala Glu Ile Ser Ala           
        195                 200                 205                       

gcg gcc aag ggt aca caa agc agt ggt ggc ctg ttt ggc tgg ctg aag        672
Ala Ala Lys Gly Thr Gln Ser Ser Gly Gly Leu Phe Gly Trp Leu Lys           
    210                 215                 220                           

aaa ctg ttc agc taa                                                    687
Lys Leu Phe Ser                                                           
225                                                                       


<210>  4
<211>  228
<212>  PRT
<213>  Methylobacillus flagellatum

<400>  4

Val Ala Lys Pro Leu Val Gln Met Ala Leu Asp Ser Leu Asp Phe Asp 
1               5                   10                  15      


Gln Thr Val Ala Leu Ala Thr Thr Val Ala Pro His Val Asp Ile Leu 
            20                  25                  30          


Glu Ile Gly Thr Pro Cys Ile Lys Tyr Asn Gly Ile Lys Leu Leu Glu 
        35                  40                  45              


Thr Leu Arg Ala Lys Phe Pro Asn Asn Lys Ile Leu Val Asp Leu Lys 
    50                  55                  60                  


Thr Met Asp Ala Gly Phe Tyr Glu Ala Glu Pro Phe Tyr Lys Ala Gly 
65                  70                  75                  80  


Ala Asp Ile Val Thr Val Leu Gly Thr Ala Asp Ile Gly Thr Ile Lys 
                85                  90                  95      


Gly Val Ile Asp Val Ala Asn Lys Tyr Gly Lys Lys Ala Gln Val Asp 
            100                 105                 110         


Leu Ile Asn Val Thr Asp Lys Ala Ala Arg Thr Lys Glu Val Ala Lys 
        115                 120                 125             


Leu Gly Ala His Ile Ile Gly Val His Thr Gly Leu Asp Gln Gln Ala 
    130                 135                 140                 


Ala Gly Gln Thr Pro Phe Ala Asp Leu Asn Leu Val Ser Ser Leu Asn 
145                 150                 155                 160 


Leu Gly Val Asp Ile Ser Val Ala Gly Gly Val Lys Ala Thr Thr Ala 
                165                 170                 175     


Lys Gln Val Val Asp Ala Gly Ala Thr Ile Val Val Ala Gly Ala Ala 
            180                 185                 190         


Ile Tyr Gly Ala Ala Asp Pro Ala Ala Ala Ala Ala Glu Ile Ser Ala 
        195                 200                 205             


Ala Ala Lys Gly Thr Gln Ser Ser Gly Gly Leu Phe Gly Trp Leu Lys 
    210                 215                 220                 


Lys Leu Phe Ser 
225             


<210>  5
<211>  1152
<212>  DNA
<213>  Bacillus methanolicus


<220>
<221>  CDS
<222>  (1)..(1152)

<400>  5
atg acg caa aga aac ttt ttc att cca cca gct agc gta att gga cgc         48
Met Thr Gln Arg Asn Phe Phe Ile Pro Pro Ala Ser Val Ile Gly Arg           
1               5                   10                  15                

ggc gct gta aaa gaa gta gga aca aga ctt aag caa att gga gct aca         96
Gly Ala Val Lys Glu Val Gly Thr Arg Leu Lys Gln Ile Gly Ala Thr           
            20                  25                  30                    

aaa gca ctt atc gtt aca gat gca ttt ctt cat ggc aca ggt ttg tca        144
Lys Ala Leu Ile Val Thr Asp Ala Phe Leu His Gly Thr Gly Leu Ser           
        35                  40                  45                        

gaa gaa gtt gct aaa aac att cgt gaa gct ggc ctt gat gct gta att        192
Glu Glu Val Ala Lys Asn Ile Arg Glu Ala Gly Leu Asp Ala Val Ile           
    50                  55                  60                            

ttc cca aaa gct caa cca gat cca gca gat aca caa gtt cat gaa ggc        240
Phe Pro Lys Ala Gln Pro Asp Pro Ala Asp Thr Gln Val His Glu Gly           
65                  70                  75                  80            

gta gat ata ttc aaa caa gaa aaa tgt gat gca ctt gtt tct atc ggt        288
Val Asp Ile Phe Lys Gln Glu Lys Cys Asp Ala Leu Val Ser Ile Gly           
                85                  90                  95                

gga ggt agc tct cac gat aca gca aaa gca atc ggt tta gtt gca gca        336
Gly Gly Ser Ser His Asp Thr Ala Lys Ala Ile Gly Leu Val Ala Ala           
            100                 105                 110                   

aac ggc gga aga atc aac gac tat caa ggt gta aac agt gta gaa aaa        384
Asn Gly Gly Arg Ile Asn Asp Tyr Gln Gly Val Asn Ser Val Glu Lys           
        115                 120                 125                       

ccg gtt gtt cca gta gtt gca atc act aca aca gct ggt act ggt agt        432
Pro Val Val Pro Val Val Ala Ile Thr Thr Thr Ala Gly Thr Gly Ser           
    130                 135                 140                           

gaa aca aca tct ctt gcg gtt att aca gat tct gca cgt aaa gta aaa        480
Glu Thr Thr Ser Leu Ala Val Ile Thr Asp Ser Ala Arg Lys Val Lys           
145                 150                 155                 160           

atg cca gtt atc gat gag aaa att aca cca act gta gca att gtt gac        528
Met Pro Val Ile Asp Glu Lys Ile Thr Pro Thr Val Ala Ile Val Asp           
                165                 170                 175               

cca gaa tta atg gtg aaa aaa cca gct gga tta aca att gca act ggt        576
Pro Glu Leu Met Val Lys Lys Pro Ala Gly Leu Thr Ile Ala Thr Gly           
            180                 185                 190                   

atg gat gca tta tcc cat gca att gaa gca tat gtt gca aaa cgt gct        624
Met Asp Ala Leu Ser His Ala Ile Glu Ala Tyr Val Ala Lys Arg Ala           
        195                 200                 205                       

aca cca gtt act gat gcg ttt gca att caa gca atg aaa ctc att aat        672
Thr Pro Val Thr Asp Ala Phe Ala Ile Gln Ala Met Lys Leu Ile Asn           
    210                 215                 220                           

gaa tac tta cca cgt gcg gtt gca aat gga gaa gac atc gaa gca cgt        720
Glu Tyr Leu Pro Arg Ala Val Ala Asn Gly Glu Asp Ile Glu Ala Arg           
225                 230                 235                 240           

gaa gca atg gct tat gca caa tac atg gca gga gtg gca ttt aac aac        768
Glu Ala Met Ala Tyr Ala Gln Tyr Met Ala Gly Val Ala Phe Asn Asn           
                245                 250                 255               

gga ggt tta gga tta gta cac tct att tct cac caa gta ggt gga gtt        816
Gly Gly Leu Gly Leu Val His Ser Ile Ser His Gln Val Gly Gly Val           
            260                 265                 270                   

tac aag tta caa cac gga atc tgt aac tca gtt aat atg cca cac gtt        864
Tyr Lys Leu Gln His Gly Ile Cys Asn Ser Val Asn Met Pro His Val           
        275                 280                 285                       

tgc caa ttc aac tta att gct cgt act gaa cgc ttc gca cac att gct        912
Cys Gln Phe Asn Leu Ile Ala Arg Thr Glu Arg Phe Ala His Ile Ala           
    290                 295                 300                           

gag ctt tta ggc gag aat gtt tct ggc tta agc act gca tct gct gct        960
Glu Leu Leu Gly Glu Asn Val Ser Gly Leu Ser Thr Ala Ser Ala Ala           
305                 310                 315                 320           

gag aga gca att gta gcg ctt caa cgc tat aac aaa aac ttc ggt atc       1008
Glu Arg Ala Ile Val Ala Leu Gln Arg Tyr Asn Lys Asn Phe Gly Ile           
                325                 330                 335               

cca tct ggc tat gca gaa atg ggc gta aaa gaa gag gat atc gaa tta       1056
Pro Ser Gly Tyr Ala Glu Met Gly Val Lys Glu Glu Asp Ile Glu Leu           
            340                 345                 350                   

tta gcg aac aac gcg tac caa gac gta tgt act cta gat aac cca cgt       1104
Leu Ala Asn Asn Ala Tyr Gln Asp Val Cys Thr Leu Asp Asn Pro Arg           
        355                 360                 365                       

gtt cct act gtt caa gac att gca caa atc atc aaa aac gct ctg taa       1152
Val Pro Thr Val Gln Asp Ile Ala Gln Ile Ile Lys Asn Ala Leu               
    370                 375                 380                           


<210>  6
<211>  383
<212>  PRT
<213>  Bacillus methanolicus

<400>  6

Met Thr Gln Arg Asn Phe Phe Ile Pro Pro Ala Ser Val Ile Gly Arg 
1               5                   10                  15      


Gly Ala Val Lys Glu Val Gly Thr Arg Leu Lys Gln Ile Gly Ala Thr 
            20                  25                  30          


Lys Ala Leu Ile Val Thr Asp Ala Phe Leu His Gly Thr Gly Leu Ser 
        35                  40                  45              


Glu Glu Val Ala Lys Asn Ile Arg Glu Ala Gly Leu Asp Ala Val Ile 
    50                  55                  60                  


Phe Pro Lys Ala Gln Pro Asp Pro Ala Asp Thr Gln Val His Glu Gly 
65                  70                  75                  80  


Val Asp Ile Phe Lys Gln Glu Lys Cys Asp Ala Leu Val Ser Ile Gly 
                85                  90                  95      


Gly Gly Ser Ser His Asp Thr Ala Lys Ala Ile Gly Leu Val Ala Ala 
            100                 105                 110         


Asn Gly Gly Arg Ile Asn Asp Tyr Gln Gly Val Asn Ser Val Glu Lys 
        115                 120                 125             


Pro Val Val Pro Val Val Ala Ile Thr Thr Thr Ala Gly Thr Gly Ser 
    130                 135                 140                 


Glu Thr Thr Ser Leu Ala Val Ile Thr Asp Ser Ala Arg Lys Val Lys 
145                 150                 155                 160 


Met Pro Val Ile Asp Glu Lys Ile Thr Pro Thr Val Ala Ile Val Asp 
                165                 170                 175     


Pro Glu Leu Met Val Lys Lys Pro Ala Gly Leu Thr Ile Ala Thr Gly 
            180                 185                 190         


Met Asp Ala Leu Ser His Ala Ile Glu Ala Tyr Val Ala Lys Arg Ala 
        195                 200                 205             


Thr Pro Val Thr Asp Ala Phe Ala Ile Gln Ala Met Lys Leu Ile Asn 
    210                 215                 220                 


Glu Tyr Leu Pro Arg Ala Val Ala Asn Gly Glu Asp Ile Glu Ala Arg 
225                 230                 235                 240 


Glu Ala Met Ala Tyr Ala Gln Tyr Met Ala Gly Val Ala Phe Asn Asn 
                245                 250                 255     


Gly Gly Leu Gly Leu Val His Ser Ile Ser His Gln Val Gly Gly Val 
            260                 265                 270         


Tyr Lys Leu Gln His Gly Ile Cys Asn Ser Val Asn Met Pro His Val 
        275                 280                 285             


Cys Gln Phe Asn Leu Ile Ala Arg Thr Glu Arg Phe Ala His Ile Ala 
    290                 295                 300                 


Glu Leu Leu Gly Glu Asn Val Ser Gly Leu Ser Thr Ala Ser Ala Ala 
305                 310                 315                 320 


Glu Arg Ala Ile Val Ala Leu Gln Arg Tyr Asn Lys Asn Phe Gly Ile 
                325                 330                 335     


Pro Ser Gly Tyr Ala Glu Met Gly Val Lys Glu Glu Asp Ile Glu Leu 
            340                 345                 350         


Leu Ala Asn Asn Ala Tyr Gln Asp Val Cys Thr Leu Asp Asn Pro Arg 
        355                 360                 365             


Val Pro Thr Val Gln Asp Ile Ala Gln Ile Ile Lys Asn Ala Leu 
    370                 375                 380             


<210>  7
<211>  1650
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1650)

<400>  7
atg aaa aac atc aat cca acg cag acc gct gcc tgg cag gca cta cag         48
Met Lys Asn Ile Asn Pro Thr Gln Thr Ala Ala Trp Gln Ala Leu Gln           
1               5                   10                  15                

aaa cac ttc gat gaa atg aaa gac gtt acg atc gcc gat ctt ttt gct         96
Lys His Phe Asp Glu Met Lys Asp Val Thr Ile Ala Asp Leu Phe Ala           
            20                  25                  30                    

aaa gac ggc gat cgt ttt tct aag ttc tcc gca acc ttc gac gat cag        144
Lys Asp Gly Asp Arg Phe Ser Lys Phe Ser Ala Thr Phe Asp Asp Gln           
        35                  40                  45                        

atg ctg gtg gat tac tcc aaa aac cgc atc act gaa gag acg ctg gcg        192
Met Leu Val Asp Tyr Ser Lys Asn Arg Ile Thr Glu Glu Thr Leu Ala           
    50                  55                  60                            

aaa tta cag gat ctg gcg aaa gag tgc gat ctg gcg ggc gcg att aag        240
Lys Leu Gln Asp Leu Ala Lys Glu Cys Asp Leu Ala Gly Ala Ile Lys           
65                  70                  75                  80            

tcg atg ttc tct ggc gag aag atc aac cgc act gaa aac cgc gcc gtg        288
Ser Met Phe Ser Gly Glu Lys Ile Asn Arg Thr Glu Asn Arg Ala Val           
                85                  90                  95                

ctg cac gta gcg ctg cgt aac cgt agc aat acc ccg att ttg gtt gat        336
Leu His Val Ala Leu Arg Asn Arg Ser Asn Thr Pro Ile Leu Val Asp           
            100                 105                 110                   

ggc aaa gac gta atg ccg gaa gtc aac gcg gtg ctg gag aag atg aaa        384
Gly Lys Asp Val Met Pro Glu Val Asn Ala Val Leu Glu Lys Met Lys           
        115                 120                 125                       

acc ttc tca gaa gcg att att tcc ggt gag tgg aaa ggt tat acc ggc        432
Thr Phe Ser Glu Ala Ile Ile Ser Gly Glu Trp Lys Gly Tyr Thr Gly           
    130                 135                 140                           

aaa gca atc act gac gta gtg aac atc ggg atc ggc ggt tct gac ctc        480
Lys Ala Ile Thr Asp Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu           
145                 150                 155                 160           

ggc cca tac atg gtg acc gaa gct ctg cgt ccg tac aaa aac cac ctg        528
Gly Pro Tyr Met Val Thr Glu Ala Leu Arg Pro Tyr Lys Asn His Leu           
                165                 170                 175               

aac atg cac ttt gtt tct aac gtc gat ggg act cac atc gcg gaa gtg        576
Asn Met His Phe Val Ser Asn Val Asp Gly Thr His Ile Ala Glu Val           
            180                 185                 190                   

ctg aaa aaa gta aac ccg gaa acc acg ctg ttc ctg gta gca tct aaa        624
Leu Lys Lys Val Asn Pro Glu Thr Thr Leu Phe Leu Val Ala Ser Lys           
        195                 200                 205                       

acc ttc acc act cag gaa act atg acc aac gcc cat agc gcg cgt gac        672
Thr Phe Thr Thr Gln Glu Thr Met Thr Asn Ala His Ser Ala Arg Asp           
    210                 215                 220                           

tgg ttc ctg aaa gcg gca ggt gat gaa aaa cac gtt gca aaa cac ttt        720
Trp Phe Leu Lys Ala Ala Gly Asp Glu Lys His Val Ala Lys His Phe           
225                 230                 235                 240           

gcg gcg ctt tcc acc aat gcc aaa gcc gtt ggc gag ttt ggt att gat        768
Ala Ala Leu Ser Thr Asn Ala Lys Ala Val Gly Glu Phe Gly Ile Asp           
                245                 250                 255               

act gcc aac atg ttc gag ttc tgg gac tgg gtt ggc ggc cgt tac tct        816
Thr Ala Asn Met Phe Glu Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser           
            260                 265                 270                   

ttg tgg tca gcg att ggc ctg tcg att gtt ctc tcc atc ggc ttt gat        864
Leu Trp Ser Ala Ile Gly Leu Ser Ile Val Leu Ser Ile Gly Phe Asp           
        275                 280                 285                       

aac ttc gtt gaa ctg ctt tcc ggc gca cac gcg atg gac aag cat ttc        912
Asn Phe Val Glu Leu Leu Ser Gly Ala His Ala Met Asp Lys His Phe           
    290                 295                 300                           

tcc acc acg cct gcc gag aaa aac ctg cct gta ctg ctg gcg ctg att        960
Ser Thr Thr Pro Ala Glu Lys Asn Leu Pro Val Leu Leu Ala Leu Ile           
305                 310                 315                 320           

ggc atc tgg tac aac aat ttc ttt ggt gcg gaa act gaa gcg att ctg       1008
Gly Ile Trp Tyr Asn Asn Phe Phe Gly Ala Glu Thr Glu Ala Ile Leu           
                325                 330                 335               

ccg tat gac cag tat atg cac cgt ttc gcg gcg tac ttc cag cag ggc       1056
Pro Tyr Asp Gln Tyr Met His Arg Phe Ala Ala Tyr Phe Gln Gln Gly           
            340                 345                 350                   

aat atg gag tcc aac ggt aag tat gtt gac cgt aac ggt aac gtt gtg       1104
Asn Met Glu Ser Asn Gly Lys Tyr Val Asp Arg Asn Gly Asn Val Val           
        355                 360                 365                       

gat tac cag act ggc ccg att atc tgg ggt gaa cca ggc act aac ggt       1152
Asp Tyr Gln Thr Gly Pro Ile Ile Trp Gly Glu Pro Gly Thr Asn Gly           
    370                 375                 380                           

cag cac gcg ttc tac cag ctg atc cac cag gga acc aaa atg gta ccg       1200
Gln His Ala Phe Tyr Gln Leu Ile His Gln Gly Thr Lys Met Val Pro           
385                 390                 395                 400           

tgc gat ttc atc gct ccg gct atc acc cat aac ccg ctc tct gat cat       1248
Cys Asp Phe Ile Ala Pro Ala Ile Thr His Asn Pro Leu Ser Asp His           
                405                 410                 415               

cac cag aaa ctg ctg tct aac ttc ttc gcc cag acc gaa gcg ctg gcg       1296
His Gln Lys Leu Leu Ser Asn Phe Phe Ala Gln Thr Glu Ala Leu Ala           
            420                 425                 430                   

ttt ggt aaa tcc cgc gaa gtg gtt gag cag gaa tat cgt gat cag ggt       1344
Phe Gly Lys Ser Arg Glu Val Val Glu Gln Glu Tyr Arg Asp Gln Gly           
        435                 440                 445                       

aaa gat ccg gca acg ctt gac tac gtg gtg ccg ttc aaa gta ttc gaa       1392
Lys Asp Pro Ala Thr Leu Asp Tyr Val Val Pro Phe Lys Val Phe Glu           
    450                 455                 460                           

ggt aac cgc ccg acc aac tcc atc ctg ctg cgt gaa atc act ccg ttc       1440
Gly Asn Arg Pro Thr Asn Ser Ile Leu Leu Arg Glu Ile Thr Pro Phe           
465                 470                 475                 480           

agc ctg ggt gcg ttg att gcg ctg tat gag cac aaa atc ttt act cag       1488
Ser Leu Gly Ala Leu Ile Ala Leu Tyr Glu His Lys Ile Phe Thr Gln           
                485                 490                 495               

ggc gtg atc ctg aac atc ttc acc ttc gac cag tgg ggc gtg gaa ctg       1536
Gly Val Ile Leu Asn Ile Phe Thr Phe Asp Gln Trp Gly Val Glu Leu           
            500                 505                 510                   

ggt aaa cag ctg gcg aac cgt att ctg cca gag ctg aaa gat gat aaa       1584
Gly Lys Gln Leu Ala Asn Arg Ile Leu Pro Glu Leu Lys Asp Asp Lys           
        515                 520                 525                       

gaa atc agc agc cac gat agc tcg acc aat ggt ctg att aac cgc tat       1632
Glu Ile Ser Ser His Asp Ser Ser Thr Asn Gly Leu Ile Asn Arg Tyr           
    530                 535                 540                           

aaa gcg tgg cgc ggt taa                                               1650
Lys Ala Trp Arg Gly                                                       
545                                                                       


<210>  8
<211>  549
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Lys Asn Ile Asn Pro Thr Gln Thr Ala Ala Trp Gln Ala Leu Gln 
1               5                   10                  15      


Lys His Phe Asp Glu Met Lys Asp Val Thr Ile Ala Asp Leu Phe Ala 
            20                  25                  30          


Lys Asp Gly Asp Arg Phe Ser Lys Phe Ser Ala Thr Phe Asp Asp Gln 
        35                  40                  45              


Met Leu Val Asp Tyr Ser Lys Asn Arg Ile Thr Glu Glu Thr Leu Ala 
    50                  55                  60                  


Lys Leu Gln Asp Leu Ala Lys Glu Cys Asp Leu Ala Gly Ala Ile Lys 
65                  70                  75                  80  


Ser Met Phe Ser Gly Glu Lys Ile Asn Arg Thr Glu Asn Arg Ala Val 
                85                  90                  95      


Leu His Val Ala Leu Arg Asn Arg Ser Asn Thr Pro Ile Leu Val Asp 
            100                 105                 110         


Gly Lys Asp Val Met Pro Glu Val Asn Ala Val Leu Glu Lys Met Lys 
        115                 120                 125             


Thr Phe Ser Glu Ala Ile Ile Ser Gly Glu Trp Lys Gly Tyr Thr Gly 
    130                 135                 140                 


Lys Ala Ile Thr Asp Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu 
145                 150                 155                 160 


Gly Pro Tyr Met Val Thr Glu Ala Leu Arg Pro Tyr Lys Asn His Leu 
                165                 170                 175     


Asn Met His Phe Val Ser Asn Val Asp Gly Thr His Ile Ala Glu Val 
            180                 185                 190         


Leu Lys Lys Val Asn Pro Glu Thr Thr Leu Phe Leu Val Ala Ser Lys 
        195                 200                 205             


Thr Phe Thr Thr Gln Glu Thr Met Thr Asn Ala His Ser Ala Arg Asp 
    210                 215                 220                 


Trp Phe Leu Lys Ala Ala Gly Asp Glu Lys His Val Ala Lys His Phe 
225                 230                 235                 240 


Ala Ala Leu Ser Thr Asn Ala Lys Ala Val Gly Glu Phe Gly Ile Asp 
                245                 250                 255     


Thr Ala Asn Met Phe Glu Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser 
            260                 265                 270         


Leu Trp Ser Ala Ile Gly Leu Ser Ile Val Leu Ser Ile Gly Phe Asp 
        275                 280                 285             


Asn Phe Val Glu Leu Leu Ser Gly Ala His Ala Met Asp Lys His Phe 
    290                 295                 300                 


Ser Thr Thr Pro Ala Glu Lys Asn Leu Pro Val Leu Leu Ala Leu Ile 
305                 310                 315                 320 


Gly Ile Trp Tyr Asn Asn Phe Phe Gly Ala Glu Thr Glu Ala Ile Leu 
                325                 330                 335     


Pro Tyr Asp Gln Tyr Met His Arg Phe Ala Ala Tyr Phe Gln Gln Gly 
            340                 345                 350         


Asn Met Glu Ser Asn Gly Lys Tyr Val Asp Arg Asn Gly Asn Val Val 
        355                 360                 365             


Asp Tyr Gln Thr Gly Pro Ile Ile Trp Gly Glu Pro Gly Thr Asn Gly 
    370                 375                 380                 


Gln His Ala Phe Tyr Gln Leu Ile His Gln Gly Thr Lys Met Val Pro 
385                 390                 395                 400 


Cys Asp Phe Ile Ala Pro Ala Ile Thr His Asn Pro Leu Ser Asp His 
                405                 410                 415     


His Gln Lys Leu Leu Ser Asn Phe Phe Ala Gln Thr Glu Ala Leu Ala 
            420                 425                 430         


Phe Gly Lys Ser Arg Glu Val Val Glu Gln Glu Tyr Arg Asp Gln Gly 
        435                 440                 445             


Lys Asp Pro Ala Thr Leu Asp Tyr Val Val Pro Phe Lys Val Phe Glu 
    450                 455                 460                 


Gly Asn Arg Pro Thr Asn Ser Ile Leu Leu Arg Glu Ile Thr Pro Phe 
465                 470                 475                 480 


Ser Leu Gly Ala Leu Ile Ala Leu Tyr Glu His Lys Ile Phe Thr Gln 
                485                 490                 495     


Gly Val Ile Leu Asn Ile Phe Thr Phe Asp Gln Trp Gly Val Glu Leu 
            500                 505                 510         


Gly Lys Gln Leu Ala Asn Arg Ile Leu Pro Glu Leu Lys Asp Asp Lys 
        515                 520                 525             


Glu Ile Ser Ser His Asp Ser Ser Thr Asn Gly Leu Ile Asn Arg Tyr 
    530                 535                 540                 


Lys Ala Trp Arg Gly 
545                 


<210>  9
<211>  1638
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutant PGi derived from E. coli


<220>
<221>  CDS
<222>  (1)..(1638)

<400>  9
atg aaa aac atc aat cca acg cag acc gct gcc tgg cag gca cta cag         48
Met Lys Asn Ile Asn Pro Thr Gln Thr Ala Ala Trp Gln Ala Leu Gln           
1               5                   10                  15                

aaa cac ttc gat gaa atg aaa gac gtt acg atc gcc gat ctt ttt gct         96
Lys His Phe Asp Glu Met Lys Asp Val Thr Ile Ala Asp Leu Phe Ala           
            20                  25                  30                    

aaa gac ggc gat cgt ttt tct aag ttc tcc gca acc ttc gac gat cag        144
Lys Asp Gly Asp Arg Phe Ser Lys Phe Ser Ala Thr Phe Asp Asp Gln           
        35                  40                  45                        

atg ctg gtg gat tac tcc aaa aac cgc atc act gaa gag acg ctg gcg        192
Met Leu Val Asp Tyr Ser Lys Asn Arg Ile Thr Glu Glu Thr Leu Ala           
    50                  55                  60                            

aaa tta cag gat ctg gcg aaa gag tgc gat ctg gcg ggc gcg att aag        240
Lys Leu Gln Asp Leu Ala Lys Glu Cys Asp Leu Ala Gly Ala Ile Lys           
65                  70                  75                  80            

tcg atg ttc tct ggc gag aag atc aac cgc act gaa aac cgc gcc gtg        288
Ser Met Phe Ser Gly Glu Lys Ile Asn Arg Thr Glu Asn Arg Ala Val           
                85                  90                  95                

ctg cac gta gcg ctg cgt aac cgt agc aat acc ccg att ttg gtt gat        336
Leu His Val Ala Leu Arg Asn Arg Ser Asn Thr Pro Ile Leu Val Asp           
            100                 105                 110                   

ggc aaa gac gta atg ccg gaa gtc aac gcg gtg ctg gag aag atg aaa        384
Gly Lys Asp Val Met Pro Glu Val Asn Ala Val Leu Glu Lys Met Lys           
        115                 120                 125                       

acc ttc tca gaa gcg att att tcc ggt gag tgg aaa ggt tat acc ggc        432
Thr Phe Ser Glu Ala Ile Ile Ser Gly Glu Trp Lys Gly Tyr Thr Gly           
    130                 135                 140                           

aaa gca atc act gac gta gtg aac atc ggg atc ggc ggt tct gac ctc        480
Lys Ala Ile Thr Asp Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu           
145                 150                 155                 160           

ggc cca tac atg gtg acc gaa gct ctg cgt ccg tac aaa aac cac ctg        528
Gly Pro Tyr Met Val Thr Glu Ala Leu Arg Pro Tyr Lys Asn His Leu           
                165                 170                 175               

aac atg cac ttt gtt tct aac gtc gat ggg act cac atc gcg gaa gtg        576
Asn Met His Phe Val Ser Asn Val Asp Gly Thr His Ile Ala Glu Val           
            180                 185                 190                   

ctg aaa aaa gta aac ccg gaa acc acg ctg ttc ctg gta gca tct aaa        624
Leu Lys Lys Val Asn Pro Glu Thr Thr Leu Phe Leu Val Ala Ser Lys           
        195                 200                 205                       

acc ttc acc act cag gaa act atg acc aac gcc cat agc gcg cgt gac        672
Thr Phe Thr Thr Gln Glu Thr Met Thr Asn Ala His Ser Ala Arg Asp           
    210                 215                 220                           

tgg ttc ctg aaa gcg gca ggt gat gaa aaa cac ttt gcg gcg ctt tcc        720
Trp Phe Leu Lys Ala Ala Gly Asp Glu Lys His Phe Ala Ala Leu Ser           
225                 230                 235                 240           

acc aat gcc aaa gcc gtt ggc gag ttt ggt att gat act gcc aac atg        768
Thr Asn Ala Lys Ala Val Gly Glu Phe Gly Ile Asp Thr Ala Asn Met           
                245                 250                 255               

ttc gag ttc tgg gac tgg gtt ggc ggc cgt tac tct ttg tgg tca gcg        816
Phe Glu Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Leu Trp Ser Ala           
            260                 265                 270                   

att ggc ctg tcg att gtt ctc tcc atc ggc ttt gat aac ttc gtt gaa        864
Ile Gly Leu Ser Ile Val Leu Ser Ile Gly Phe Asp Asn Phe Val Glu           
        275                 280                 285                       

ctg ctt tcc ggc gca cac gcg atg gac aag cat ttc tcc acc acg cct        912
Leu Leu Ser Gly Ala His Ala Met Asp Lys His Phe Ser Thr Thr Pro           
    290                 295                 300                           

gcc gag aaa aac ctg cct gta ctg ctg gcg ctg att ggc atc tgg tac        960
Ala Glu Lys Asn Leu Pro Val Leu Leu Ala Leu Ile Gly Ile Trp Tyr           
305                 310                 315                 320           

aac aat ttc ttt ggt gcg gaa act gaa gcg att ctg ccg tat gac cag       1008
Asn Asn Phe Phe Gly Ala Glu Thr Glu Ala Ile Leu Pro Tyr Asp Gln           
                325                 330                 335               

tat atg cac cgt ttc gcg gcg tac ttc cag cag ggc aat atg gag tcc       1056
Tyr Met His Arg Phe Ala Ala Tyr Phe Gln Gln Gly Asn Met Glu Ser           
            340                 345                 350                   

aac ggt aag tat gtt gac cgt aac ggt aac gtt gtg gat tac cag act       1104
Asn Gly Lys Tyr Val Asp Arg Asn Gly Asn Val Val Asp Tyr Gln Thr           
        355                 360                 365                       

ggc ccg att atc tgg ggt gaa cca ggc act aac ggt cag cac gcg ttc       1152
Gly Pro Ile Ile Trp Gly Glu Pro Gly Thr Asn Gly Gln His Ala Phe           
    370                 375                 380                           

tac cag ctg atc cac cag gga acc aaa atg gta ccg tgc gat ttc atc       1200
Tyr Gln Leu Ile His Gln Gly Thr Lys Met Val Pro Cys Asp Phe Ile           
385                 390                 395                 400           

gct ccg gct atc acc cat aac ccg ctc tct gat cat cac cag aaa ctg       1248
Ala Pro Ala Ile Thr His Asn Pro Leu Ser Asp His His Gln Lys Leu           
                405                 410                 415               

ctg tct aac ttc ttc gcc cag acc gaa gcg ctg gcg ttt ggt aaa tcc       1296
Leu Ser Asn Phe Phe Ala Gln Thr Glu Ala Leu Ala Phe Gly Lys Ser           
            420                 425                 430                   

cgc gaa gtg gtt gag cag gaa tat cgt gat cag ggt aaa gat ccg gca       1344
Arg Glu Val Val Glu Gln Glu Tyr Arg Asp Gln Gly Lys Asp Pro Ala           
        435                 440                 445                       

acg ctt gac tac gtg gtg ccg ttc aaa gta ttc gaa ggt aac cgc ccg       1392
Thr Leu Asp Tyr Val Val Pro Phe Lys Val Phe Glu Gly Asn Arg Pro           
    450                 455                 460                           

acc aac tcc atc ctg ctg cgt gaa atc act ccg ttc agc ctg ggt gcg       1440
Thr Asn Ser Ile Leu Leu Arg Glu Ile Thr Pro Phe Ser Leu Gly Ala           
465                 470                 475                 480           

ttg att gcg ctg tat gag cac aaa atc ttt act cag ggc gtg atc ctg       1488
Leu Ile Ala Leu Tyr Glu His Lys Ile Phe Thr Gln Gly Val Ile Leu           
                485                 490                 495               

aac atc ttc acc ttc gac cag tgg ggc gtg gaa ctg ggt aaa cag ctg       1536
Asn Ile Phe Thr Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln Leu           
            500                 505                 510                   

gcg aac cgt att ctg cca gag ctg aaa gat gat aaa gaa atc agc agc       1584
Ala Asn Arg Ile Leu Pro Glu Leu Lys Asp Asp Lys Glu Ile Ser Ser           
        515                 520                 525                       

cac gat agc tcg acc aat ggt ctg att aac cgc tat aaa gcg tgg cgc       1632
His Asp Ser Ser Thr Asn Gly Leu Ile Asn Arg Tyr Lys Ala Trp Arg           
    530                 535                 540                           

ggt taa                                                               1638
Gly                                                                       
545                                                                       


<210>  10
<211>  545
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Lys Asn Ile Asn Pro Thr Gln Thr Ala Ala Trp Gln Ala Leu Gln 
1               5                   10                  15      


Lys His Phe Asp Glu Met Lys Asp Val Thr Ile Ala Asp Leu Phe Ala 
            20                  25                  30          


Lys Asp Gly Asp Arg Phe Ser Lys Phe Ser Ala Thr Phe Asp Asp Gln 
        35                  40                  45              


Met Leu Val Asp Tyr Ser Lys Asn Arg Ile Thr Glu Glu Thr Leu Ala 
    50                  55                  60                  


Lys Leu Gln Asp Leu Ala Lys Glu Cys Asp Leu Ala Gly Ala Ile Lys 
65                  70                  75                  80  


Ser Met Phe Ser Gly Glu Lys Ile Asn Arg Thr Glu Asn Arg Ala Val 
                85                  90                  95      


Leu His Val Ala Leu Arg Asn Arg Ser Asn Thr Pro Ile Leu Val Asp 
            100                 105                 110         


Gly Lys Asp Val Met Pro Glu Val Asn Ala Val Leu Glu Lys Met Lys 
        115                 120                 125             


Thr Phe Ser Glu Ala Ile Ile Ser Gly Glu Trp Lys Gly Tyr Thr Gly 
    130                 135                 140                 


Lys Ala Ile Thr Asp Val Val Asn Ile Gly Ile Gly Gly Ser Asp Leu 
145                 150                 155                 160 


Gly Pro Tyr Met Val Thr Glu Ala Leu Arg Pro Tyr Lys Asn His Leu 
                165                 170                 175     


Asn Met His Phe Val Ser Asn Val Asp Gly Thr His Ile Ala Glu Val 
            180                 185                 190         


Leu Lys Lys Val Asn Pro Glu Thr Thr Leu Phe Leu Val Ala Ser Lys 
        195                 200                 205             


Thr Phe Thr Thr Gln Glu Thr Met Thr Asn Ala His Ser Ala Arg Asp 
    210                 215                 220                 


Trp Phe Leu Lys Ala Ala Gly Asp Glu Lys His Phe Ala Ala Leu Ser 
225                 230                 235                 240 


Thr Asn Ala Lys Ala Val Gly Glu Phe Gly Ile Asp Thr Ala Asn Met 
                245                 250                 255     


Phe Glu Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Leu Trp Ser Ala 
            260                 265                 270         


Ile Gly Leu Ser Ile Val Leu Ser Ile Gly Phe Asp Asn Phe Val Glu 
        275                 280                 285             


Leu Leu Ser Gly Ala His Ala Met Asp Lys His Phe Ser Thr Thr Pro 
    290                 295                 300                 


Ala Glu Lys Asn Leu Pro Val Leu Leu Ala Leu Ile Gly Ile Trp Tyr 
305                 310                 315                 320 


Asn Asn Phe Phe Gly Ala Glu Thr Glu Ala Ile Leu Pro Tyr Asp Gln 
                325                 330                 335     


Tyr Met His Arg Phe Ala Ala Tyr Phe Gln Gln Gly Asn Met Glu Ser 
            340                 345                 350         


Asn Gly Lys Tyr Val Asp Arg Asn Gly Asn Val Val Asp Tyr Gln Thr 
        355                 360                 365             


Gly Pro Ile Ile Trp Gly Glu Pro Gly Thr Asn Gly Gln His Ala Phe 
    370                 375                 380                 


Tyr Gln Leu Ile His Gln Gly Thr Lys Met Val Pro Cys Asp Phe Ile 
385                 390                 395                 400 


Ala Pro Ala Ile Thr His Asn Pro Leu Ser Asp His His Gln Lys Leu 
                405                 410                 415     


Leu Ser Asn Phe Phe Ala Gln Thr Glu Ala Leu Ala Phe Gly Lys Ser 
            420                 425                 430         


Arg Glu Val Val Glu Gln Glu Tyr Arg Asp Gln Gly Lys Asp Pro Ala 
        435                 440                 445             


Thr Leu Asp Tyr Val Val Pro Phe Lys Val Phe Glu Gly Asn Arg Pro 
    450                 455                 460                 


Thr Asn Ser Ile Leu Leu Arg Glu Ile Thr Pro Phe Ser Leu Gly Ala 
465                 470                 475                 480 


Leu Ile Ala Leu Tyr Glu His Lys Ile Phe Thr Gln Gly Val Ile Leu 
                485                 490                 495     


Asn Ile Phe Thr Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln Leu 
            500                 505                 510         


Ala Asn Arg Ile Leu Pro Glu Leu Lys Asp Asp Lys Glu Ile Ser Ser 
        515                 520                 525             


His Asp Ser Ser Thr Asn Gly Leu Ile Asn Arg Tyr Lys Ala Trp Arg 
    530                 535                 540                 


Gly 
545 


<210>  11
<211>  85
<212>  PRT
<213>  Escherichia coli

<400>  11

Met Phe Gln Gln Glu Val Thr Ile Thr Ala Pro Asn Gly Leu His Thr 
1               5                   10                  15      


Arg Pro Ala Ala Gln Phe Val Lys Glu Ala Lys Gly Phe Thr Ser Glu 
            20                  25                  30          


Ile Thr Val Thr Ser Asn Gly Lys Ser Ala Ser Ala Lys Ser Leu Phe 
        35                  40                  45              


Lys Leu Gln Thr Leu Gly Leu Thr Gln Gly Thr Val Val Thr Ile Ser 
    50                  55                  60                  


Ala Glu Gly Glu Asp Glu Gln Lys Ala Val Glu His Leu Val Lys Leu 
65                  70                  75                  80  


Met Ala Glu Leu Glu 
                85  


<210>  12
<211>  232
<212>  PRT
<213>  Escherichia coli

<400>  12

Met Glu Asn Gln Pro Lys Leu Asn Ser Ser Lys Glu Val Ile Ala Phe 
1               5                   10                  15      


Leu Ala Glu Arg Phe Pro His Cys Phe Ser Ala Glu Gly Glu Ala Arg 
            20                  25                  30          


Pro Leu Lys Ile Gly Ile Phe Gln Asp Leu Val Asp Arg Val Ala Gly 
        35                  40                  45              


Glu Met Asn Leu Ser Lys Thr Gln Leu Arg Ser Ala Leu Arg Leu Tyr 
    50                  55                  60                  


Thr Ser Ser Trp Arg Tyr Leu Tyr Gly Val Lys Pro Gly Ala Thr Arg 
65                  70                  75                  80  


Val Asp Leu Asp Gly Asn Pro Cys Gly Glu Leu Asp Glu Gln His Val 
                85                  90                  95      


Glu His Ala Arg Lys Gln Leu Glu Glu Ala Lys Ala Arg Val Gln Ala 
            100                 105                 110         


Gln Arg Ala Glu Gln Gln Ala Lys Lys Arg Glu Ala Ala Ala Thr Ala 
        115                 120                 125             


Gly Glu Lys Glu Asp Ala Pro Arg Arg Glu Arg Lys Pro Arg Pro Thr 
    130                 135                 140                 


Thr Pro Arg Arg Lys Glu Gly Ala Glu Arg Lys Pro Arg Ala Gln Lys 
145                 150                 155                 160 


Pro Val Glu Lys Ala Pro Lys Thr Val Lys Ala Pro Arg Glu Glu Gln 
                165                 170                 175     


His Thr Pro Val Ser Asp Ile Ser Ala Leu Thr Val Gly Gln Ala Leu 
            180                 185                 190         


Lys Val Lys Ala Gly Gln Asn Ala Met Asp Ala Thr Val Leu Glu Ile 
        195                 200                 205             


Thr Lys Asp Gly Val Arg Val Gln Leu Asn Ser Gly Met Ser Leu Ile 
    210                 215                 220                 


Val Arg Ala Glu His Leu Val Phe 
225                 230         


<210>  13
<211>  660
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(660)

<400>  13
atg acg cag gat gaa ttg aaa aaa gca gta gga tgg gcg gca ctt cag         48
Met Thr Gln Asp Glu Leu Lys Lys Ala Val Gly Trp Ala Ala Leu Gln           
1               5                   10                  15                

tat gtt cag ccc ggc acc att gtt ggt gta ggt aca ggt tcc acc gcc         96
Tyr Val Gln Pro Gly Thr Ile Val Gly Val Gly Thr Gly Ser Thr Ala           
            20                  25                  30                    

gca cac ttt att gac gcg ctc ggt aca atg aaa ggc cag att gaa ggg        144
Ala His Phe Ile Asp Ala Leu Gly Thr Met Lys Gly Gln Ile Glu Gly           
        35                  40                  45                        

gcc gtt tcc agt tca gat gct tcc act gaa aaa ctg aaa agc ctc ggc        192
Ala Val Ser Ser Ser Asp Ala Ser Thr Glu Lys Leu Lys Ser Leu Gly           
    50                  55                  60                            

att cac gtt ttt gat ctc aac gaa gtc gac agc ctt ggc atc tac gtt        240
Ile His Val Phe Asp Leu Asn Glu Val Asp Ser Leu Gly Ile Tyr Val           
65                  70                  75                  80            

gat ggc gca gat gaa atc aac ggc cac atg caa atg atc aaa ggc ggc        288
Asp Gly Ala Asp Glu Ile Asn Gly His Met Gln Met Ile Lys Gly Gly           
                85                  90                  95                

ggc gcg gcg ctg acc cgt gaa aaa atc att gct tcg gtt gca gaa aaa        336
Gly Ala Ala Leu Thr Arg Glu Lys Ile Ile Ala Ser Val Ala Glu Lys           
            100                 105                 110                   

ttt atc tgt att gca gac gct tcc aag cag gtt gat att ctg ggt aaa        384
Phe Ile Cys Ile Ala Asp Ala Ser Lys Gln Val Asp Ile Leu Gly Lys           
        115                 120                 125                       

ttc ccg ctg cca gta gaa gtt atc ccg atg gca cgt agt gca gtg gcg        432
Phe Pro Leu Pro Val Glu Val Ile Pro Met Ala Arg Ser Ala Val Ala           
    130                 135                 140                           

cgt cag ctg gtg aaa ctg ggc ggt cgt ccg gaa tac cgt cag ggc gtg        480
Arg Gln Leu Val Lys Leu Gly Gly Arg Pro Glu Tyr Arg Gln Gly Val           
145                 150                 155                 160           

gtg acc gat aat ggc aac gtg atc ctc gac gtc cac ggc atg gaa atc        528
Val Thr Asp Asn Gly Asn Val Ile Leu Asp Val His Gly Met Glu Ile           
                165                 170                 175               

ctt gac ccg ata gcg atg gaa aac gcc ata aat gcg att cct ggc gtg        576
Leu Asp Pro Ile Ala Met Glu Asn Ala Ile Asn Ala Ile Pro Gly Val           
            180                 185                 190                   

gtg act gtt ggc ttg ttt gct aac cgt ggc gcg gac gtt gcg ctg att        624
Val Thr Val Gly Leu Phe Ala Asn Arg Gly Ala Asp Val Ala Leu Ile           
        195                 200                 205                       

ggc aca cct gac ggt gtc aaa acc att gtg aaa tga                        660
Gly Thr Pro Asp Gly Val Lys Thr Ile Val Lys                               
    210                 215                                               


<210>  14
<211>  219
<212>  PRT
<213>  Escherichia coli

<400>  14

Met Thr Gln Asp Glu Leu Lys Lys Ala Val Gly Trp Ala Ala Leu Gln 
1               5                   10                  15      


Tyr Val Gln Pro Gly Thr Ile Val Gly Val Gly Thr Gly Ser Thr Ala 
            20                  25                  30          


Ala His Phe Ile Asp Ala Leu Gly Thr Met Lys Gly Gln Ile Glu Gly 
        35                  40                  45              


Ala Val Ser Ser Ser Asp Ala Ser Thr Glu Lys Leu Lys Ser Leu Gly 
    50                  55                  60                  


Ile His Val Phe Asp Leu Asn Glu Val Asp Ser Leu Gly Ile Tyr Val 
65                  70                  75                  80  


Asp Gly Ala Asp Glu Ile Asn Gly His Met Gln Met Ile Lys Gly Gly 
                85                  90                  95      


Gly Ala Ala Leu Thr Arg Glu Lys Ile Ile Ala Ser Val Ala Glu Lys 
            100                 105                 110         


Phe Ile Cys Ile Ala Asp Ala Ser Lys Gln Val Asp Ile Leu Gly Lys 
        115                 120                 125             


Phe Pro Leu Pro Val Glu Val Ile Pro Met Ala Arg Ser Ala Val Ala 
    130                 135                 140                 


Arg Gln Leu Val Lys Leu Gly Gly Arg Pro Glu Tyr Arg Gln Gly Val 
145                 150                 155                 160 


Val Thr Asp Asn Gly Asn Val Ile Leu Asp Val His Gly Met Glu Ile 
                165                 170                 175     


Leu Asp Pro Ile Ala Met Glu Asn Ala Ile Asn Ala Ile Pro Gly Val 
            180                 185                 190         


Val Thr Val Gly Leu Phe Ala Asn Arg Gly Ala Asp Val Ala Leu Ile 
        195                 200                 205             


Gly Thr Pro Asp Gly Val Lys Thr Ile Val Lys 
    210                 215                 


<210>  15
<211>  333
<212>  PRT
<213>  Escherichia coli

<400>  15

Met Ser Lys Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Leu Val 
1               5                   10                  15      


Leu Arg Arg Leu Leu Glu Val Lys Ser Asn Ile Asp Val Val Ala Ile 
            20                  25                  30          


Asn Asp Leu Thr Ser Pro Lys Ile Leu Ala Tyr Leu Leu Lys His Asp 
        35                  40                  45              


Ser Asn Tyr Gly Pro Phe Pro Trp Ser Val Asp Phe Thr Glu Asp Ser 
    50                  55                  60                  


Leu Ile Val Asp Gly Lys Ser Ile Ala Val Tyr Ala Glu Lys Glu Ala 
65                  70                  75                  80  


Lys Asn Ile Pro Trp Lys Ala Lys Gly Ala Glu Ile Ile Val Glu Cys 
                85                  90                  95      


Thr Gly Phe Tyr Thr Ser Ala Glu Lys Ser Gln Ala His Leu Asp Ala 
            100                 105                 110         


Gly Ala Lys Lys Val Leu Ile Ser Ala Pro Ala Gly Glu Met Lys Thr 
        115                 120                 125             


Ile Val Tyr Asn Val Asn Asp Asp Thr Leu Asp Gly Asn Asp Thr Ile 
    130                 135                 140                 


Val Ser Val Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Met Ala Lys 
145                 150                 155                 160 


Ala Leu His Asp Ser Phe Gly Ile Glu Val Gly Thr Met Thr Thr Ile 
                165                 170                 175     


His Ala Tyr Thr Gly Thr Gln Ser Leu Val Asp Gly Pro Arg Gly Lys 
            180                 185                 190         


Asp Leu Arg Ala Ser Arg Ala Ala Ala Glu Asn Ile Ile Pro His Thr 
        195                 200                 205             


Thr Gly Ala Ala Lys Ala Ile Gly Leu Val Ile Pro Glu Leu Ser Gly 
    210                 215                 220                 


Lys Leu Lys Gly His Ala Gln Arg Val Pro Val Lys Thr Gly Ser Val 
225                 230                 235                 240 


Thr Glu Leu Val Ser Ile Leu Gly Lys Lys Val Thr Ala Glu Glu Val 
                245                 250                 255     


Asn Asn Ala Leu Lys Gln Ala Thr Thr Asn Asn Glu Ser Phe Gly Tyr 
            260                 265                 270         


Thr Asp Glu Glu Ile Val Ser Ser Asp Ile Ile Gly Ser His Phe Gly 
        275                 280                 285             


Ser Val Phe Asp Ala Thr Gln Thr Glu Ile Thr Ala Val Gly Asp Leu 
    290                 295                 300                 


Gln Leu Val Lys Thr Val Ala Trp Tyr Asp Asn Glu Tyr Gly Phe Val 
305                 310                 315                 320 


Thr Gln Leu Ile Arg Thr Leu Glu Lys Phe Ala Lys Leu 
                325                 330             


<210>  16
<211>  954
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(954)

<400>  16
atg acg gac aaa ttg acc tcc ctt cgt cag tac acc acc gta gtg gcc         48
Met Thr Asp Lys Leu Thr Ser Leu Arg Gln Tyr Thr Thr Val Val Ala           
1               5                   10                  15                

gac act ggg gac atc gcg gca atg aag ctg tat caa ccg cag gat gcc         96
Asp Thr Gly Asp Ile Ala Ala Met Lys Leu Tyr Gln Pro Gln Asp Ala           
            20                  25                  30                    

aca acc aac cct tct ctc att ctt aac gca gcg cag att ccg gaa tac        144
Thr Thr Asn Pro Ser Leu Ile Leu Asn Ala Ala Gln Ile Pro Glu Tyr           
        35                  40                  45                        

cgt aag ttg att gat gat gct gtc gcc tgg gcg aaa cag cag agc aac        192
Arg Lys Leu Ile Asp Asp Ala Val Ala Trp Ala Lys Gln Gln Ser Asn           
    50                  55                  60                            

gat cgc gcg cag cag atc gtg gac gcg acc gac aaa ctg gca gta aat        240
Asp Arg Ala Gln Gln Ile Val Asp Ala Thr Asp Lys Leu Ala Val Asn           
65                  70                  75                  80            

att ggt ctg gaa atc ctg aaa ctg gtt ccg ggc cgt atc tca act gaa        288
Ile Gly Leu Glu Ile Leu Lys Leu Val Pro Gly Arg Ile Ser Thr Glu           
                85                  90                  95                

gtt gat gcg cgt ctt tcc tat gac acc gaa gcg tca att gcg aaa gca        336
Val Asp Ala Arg Leu Ser Tyr Asp Thr Glu Ala Ser Ile Ala Lys Ala           
            100                 105                 110                   

aaa cgc ctg atc aaa ctc tac aac gat gct ggt att agc aac gat cgt        384
Lys Arg Leu Ile Lys Leu Tyr Asn Asp Ala Gly Ile Ser Asn Asp Arg           
        115                 120                 125                       

att ctg atc aaa ctg gct tct acc tgg cag ggt atc cgt gct gca gaa        432
Ile Leu Ile Lys Leu Ala Ser Thr Trp Gln Gly Ile Arg Ala Ala Glu           
    130                 135                 140                           

cag ctg gaa aaa gaa ggc atc aac tgt aac ctg acc ctg ctg ttc tcc        480
Gln Leu Glu Lys Glu Gly Ile Asn Cys Asn Leu Thr Leu Leu Phe Ser           
145                 150                 155                 160           

ttc gct cag gct cgt gct tgt gcg gaa gcg ggc gtg ttc ctg atc tcg        528
Phe Ala Gln Ala Arg Ala Cys Ala Glu Ala Gly Val Phe Leu Ile Ser           
                165                 170                 175               

ccg ttt gtt ggc cgt att ctt gac tgg tac aaa gcg aat acc gat aag        576
Pro Phe Val Gly Arg Ile Leu Asp Trp Tyr Lys Ala Asn Thr Asp Lys           
            180                 185                 190                   

aaa gag tac gct ccg gca gaa gat ccg ggc gtg gtt tct gta tct gaa        624
Lys Glu Tyr Ala Pro Ala Glu Asp Pro Gly Val Val Ser Val Ser Glu           
        195                 200                 205                       

atc tac cag tac tac aaa gag cac ggt tat gaa acc gtg gtt atg ggc        672
Ile Tyr Gln Tyr Tyr Lys Glu His Gly Tyr Glu Thr Val Val Met Gly           
    210                 215                 220                           

gca agc ttc cgt aac atc ggc gaa att ctg gaa ctg gca ggc tgc gac        720
Ala Ser Phe Arg Asn Ile Gly Glu Ile Leu Glu Leu Ala Gly Cys Asp           
225                 230                 235                 240           

cgt ctg acc atc gca ccg gca ctg ctg aaa gag ctg gcg gag agc gaa        768
Arg Leu Thr Ile Ala Pro Ala Leu Leu Lys Glu Leu Ala Glu Ser Glu           
                245                 250                 255               

ggg gct atc gaa cgt aaa ctg tct tac acc ggc gaa gtg aaa gcg cgt        816
Gly Ala Ile Glu Arg Lys Leu Ser Tyr Thr Gly Glu Val Lys Ala Arg           
            260                 265                 270                   

ccg gcg cgt atc act gag tcc gag ttc ctg tgg cag cac aac cag gat        864
Pro Ala Arg Ile Thr Glu Ser Glu Phe Leu Trp Gln His Asn Gln Asp           
        275                 280                 285                       

cca atg gca gta gat aaa ctg gcg gaa ggt atc cgt aag ttt gct att        912
Pro Met Ala Val Asp Lys Leu Ala Glu Gly Ile Arg Lys Phe Ala Ile           
    290                 295                 300                           

gac cag gaa aaa ctg gaa aaa atg atc ggc gat ctg ctg taa                954
Asp Gln Glu Lys Leu Glu Lys Met Ile Gly Asp Leu Leu                       
305                 310                 315                               


<210>  17
<211>  317
<212>  PRT
<213>  Escherichia coli

<400>  17

Met Thr Asp Lys Leu Thr Ser Leu Arg Gln Tyr Thr Thr Val Val Ala 
1               5                   10                  15      


Asp Thr Gly Asp Ile Ala Ala Met Lys Leu Tyr Gln Pro Gln Asp Ala 
            20                  25                  30          


Thr Thr Asn Pro Ser Leu Ile Leu Asn Ala Ala Gln Ile Pro Glu Tyr 
        35                  40                  45              


Arg Lys Leu Ile Asp Asp Ala Val Ala Trp Ala Lys Gln Gln Ser Asn 
    50                  55                  60                  


Asp Arg Ala Gln Gln Ile Val Asp Ala Thr Asp Lys Leu Ala Val Asn 
65                  70                  75                  80  


Ile Gly Leu Glu Ile Leu Lys Leu Val Pro Gly Arg Ile Ser Thr Glu 
                85                  90                  95      


Val Asp Ala Arg Leu Ser Tyr Asp Thr Glu Ala Ser Ile Ala Lys Ala 
            100                 105                 110         


Lys Arg Leu Ile Lys Leu Tyr Asn Asp Ala Gly Ile Ser Asn Asp Arg 
        115                 120                 125             


Ile Leu Ile Lys Leu Ala Ser Thr Trp Gln Gly Ile Arg Ala Ala Glu 
    130                 135                 140                 


Gln Leu Glu Lys Glu Gly Ile Asn Cys Asn Leu Thr Leu Leu Phe Ser 
145                 150                 155                 160 


Phe Ala Gln Ala Arg Ala Cys Ala Glu Ala Gly Val Phe Leu Ile Ser 
                165                 170                 175     


Pro Phe Val Gly Arg Ile Leu Asp Trp Tyr Lys Ala Asn Thr Asp Lys 
            180                 185                 190         


Lys Glu Tyr Ala Pro Ala Glu Asp Pro Gly Val Val Ser Val Ser Glu 
        195                 200                 205             


Ile Tyr Gln Tyr Tyr Lys Glu His Gly Tyr Glu Thr Val Val Met Gly 
    210                 215                 220                 


Ala Ser Phe Arg Asn Ile Gly Glu Ile Leu Glu Leu Ala Gly Cys Asp 
225                 230                 235                 240 


Arg Leu Thr Ile Ala Pro Ala Leu Leu Lys Glu Leu Ala Glu Ser Glu 
                245                 250                 255     


Gly Ala Ile Glu Arg Lys Leu Ser Tyr Thr Gly Glu Val Lys Ala Arg 
            260                 265                 270         


Pro Ala Arg Ile Thr Glu Ser Glu Phe Leu Trp Gln His Asn Gln Asp 
        275                 280                 285             


Pro Met Ala Val Asp Lys Leu Ala Glu Gly Ile Arg Lys Phe Ala Ile 
    290                 295                 300                 


Asp Gln Glu Lys Leu Glu Lys Met Ile Gly Asp Leu Leu 
305                 310                 315         


<210>  18
<211>  1992
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1992)

<400>  18
atg tcc tca cgt aaa gag ctt gcc aat gct att cgt gcg ctg agc atg         48
Met Ser Ser Arg Lys Glu Leu Ala Asn Ala Ile Arg Ala Leu Ser Met           
1               5                   10                  15                

gac gca gta cag aaa gcc aaa tcc ggt cac ccg ggt gcc cct atg ggt         96
Asp Ala Val Gln Lys Ala Lys Ser Gly His Pro Gly Ala Pro Met Gly           
            20                  25                  30                    

atg gct gac att gcc gaa gtc ctg tgg cgt gat ttc ctg aaa cac aac        144
Met Ala Asp Ile Ala Glu Val Leu Trp Arg Asp Phe Leu Lys His Asn           
        35                  40                  45                        

ccg cag aat ccg tcc tgg gct gac cgt gac cgc ttc gtg ctg tcc aac        192
Pro Gln Asn Pro Ser Trp Ala Asp Arg Asp Arg Phe Val Leu Ser Asn           
    50                  55                  60                            

ggc cac ggc tcc atg ctg atc tac agc ctg ctg cac ctc acc ggt tac        240
Gly His Gly Ser Met Leu Ile Tyr Ser Leu Leu His Leu Thr Gly Tyr           
65                  70                  75                  80            

gat ctg ccg atg gaa gaa ctg aaa aac ttc cgt cag ctg cac tct aaa        288
Asp Leu Pro Met Glu Glu Leu Lys Asn Phe Arg Gln Leu His Ser Lys           
                85                  90                  95                

act ccg ggt cac ccg gaa gtg ggt tac acc gct ggt gtg gaa acc acc        336
Thr Pro Gly His Pro Glu Val Gly Tyr Thr Ala Gly Val Glu Thr Thr           
            100                 105                 110                   

acc ggt ccg ctg ggt cag ggt att gcc aac gca gtc ggt atg gcg att        384
Thr Gly Pro Leu Gly Gln Gly Ile Ala Asn Ala Val Gly Met Ala Ile           
        115                 120                 125                       

gca gaa aaa acg ctg gcg gcg cag ttt aac cgt ccg ggc cac gac att        432
Ala Glu Lys Thr Leu Ala Ala Gln Phe Asn Arg Pro Gly His Asp Ile           
    130                 135                 140                           

gtc gac cac tac acc tac gcc ttc atg ggc gac ggc tgc atg atg gaa        480
Val Asp His Tyr Thr Tyr Ala Phe Met Gly Asp Gly Cys Met Met Glu           
145                 150                 155                 160           

ggc atc tcc cac gaa gtt tgc tct ctg gcg ggt acg ctg aag ctg ggt        528
Gly Ile Ser His Glu Val Cys Ser Leu Ala Gly Thr Leu Lys Leu Gly           
                165                 170                 175               

aaa ctg att gca ttc tac gat gac aac ggt att tct atc gat ggt cac        576
Lys Leu Ile Ala Phe Tyr Asp Asp Asn Gly Ile Ser Ile Asp Gly His           
            180                 185                 190                   

gtt gaa ggc tgg ttc acc gac gac acc gca atg cgt ttc gaa gct tac        624
Val Glu Gly Trp Phe Thr Asp Asp Thr Ala Met Arg Phe Glu Ala Tyr           
        195                 200                 205                       

ggc tgg cac gtt att cgc gac atc gac ggt cat gac gcg gca tct atc        672
Gly Trp His Val Ile Arg Asp Ile Asp Gly His Asp Ala Ala Ser Ile           
    210                 215                 220                           

aaa cgc gca gta gaa gaa gcg cgc gca gtg act gac aaa cct tcc ctg        720
Lys Arg Ala Val Glu Glu Ala Arg Ala Val Thr Asp Lys Pro Ser Leu           
225                 230                 235                 240           

ctg atg tgc aaa acc atc atc ggt ttc ggt tcc ccg aac aaa gcc ggt        768
Leu Met Cys Lys Thr Ile Ile Gly Phe Gly Ser Pro Asn Lys Ala Gly           
                245                 250                 255               

acc cac gac tcc cac ggt gcg ccg ctg ggc gac gct gaa att gcc ctg        816
Thr His Asp Ser His Gly Ala Pro Leu Gly Asp Ala Glu Ile Ala Leu           
            260                 265                 270                   

acc cgc gaa caa ctg ggc tgg aaa tat gcg ccg ttc gaa atc ccg tct        864
Thr Arg Glu Gln Leu Gly Trp Lys Tyr Ala Pro Phe Glu Ile Pro Ser           
        275                 280                 285                       

gaa atc tat gct cag tgg gat gcg aaa gaa gca ggc cag gcg aaa gaa        912
Glu Ile Tyr Ala Gln Trp Asp Ala Lys Glu Ala Gly Gln Ala Lys Glu           
    290                 295                 300                           

tcc gca tgg aac gag aaa ttc gct gct tac gcg aaa gct tat ccg cag        960
Ser Ala Trp Asn Glu Lys Phe Ala Ala Tyr Ala Lys Ala Tyr Pro Gln           
305                 310                 315                 320           

gaa gcc gct gaa ttt acc cgc cgt atg aaa ggc gaa atg ccg tct gac       1008
Glu Ala Ala Glu Phe Thr Arg Arg Met Lys Gly Glu Met Pro Ser Asp           
                325                 330                 335               

ttc gac gct aaa gcg aaa gag ttc atc gct aaa ctg cag gct aat ccg       1056
Phe Asp Ala Lys Ala Lys Glu Phe Ile Ala Lys Leu Gln Ala Asn Pro           
            340                 345                 350                   

gcg aaa atc gcc agc cgt aaa gcg tct cag aat gct atc gaa gcg ttc       1104
Ala Lys Ile Ala Ser Arg Lys Ala Ser Gln Asn Ala Ile Glu Ala Phe           
        355                 360                 365                       

ggt ccg ctg ttg ccg gaa ttc ctc ggc ggt tct gct gac ctg gcg ccg       1152
Gly Pro Leu Leu Pro Glu Phe Leu Gly Gly Ser Ala Asp Leu Ala Pro           
    370                 375                 380                           

tct aac ctg acc ctg tgg tct ggt tct aaa gca atc aac gaa gat gct       1200
Ser Asn Leu Thr Leu Trp Ser Gly Ser Lys Ala Ile Asn Glu Asp Ala           
385                 390                 395                 400           

gcg ggt aac tac atc cac tac ggt gtt cgc gag ttc ggt atg acc gcg       1248
Ala Gly Asn Tyr Ile His Tyr Gly Val Arg Glu Phe Gly Met Thr Ala           
                405                 410                 415               

att gct aac ggt atc tcc ctg cac ggt ggc ttc ctg ccg tac acc tcc       1296
Ile Ala Asn Gly Ile Ser Leu His Gly Gly Phe Leu Pro Tyr Thr Ser           
            420                 425                 430                   

acc ttc ctg atg ttc gtg gaa tac gca cgt aac gcc gta cgt atg gct       1344
Thr Phe Leu Met Phe Val Glu Tyr Ala Arg Asn Ala Val Arg Met Ala           
        435                 440                 445                       

gcg ctg atg aaa cag cgt cag gtg atg gtt tac acc cac gac tcc atc       1392
Ala Leu Met Lys Gln Arg Gln Val Met Val Tyr Thr His Asp Ser Ile           
    450                 455                 460                           

ggt ctg ggc gaa gac ggc ccg act cac cag ccg gtt gag cag gtc gct       1440
Gly Leu Gly Glu Asp Gly Pro Thr His Gln Pro Val Glu Gln Val Ala           
465                 470                 475                 480           

tct ctg cgc gta acc ccg aac atg tct aca tgg cgt ccg tgt gac cag       1488
Ser Leu Arg Val Thr Pro Asn Met Ser Thr Trp Arg Pro Cys Asp Gln           
                485                 490                 495               

gtt gaa tcc gcg gtc gcg tgg aaa tac ggt gtt gag cgt cag gac ggc       1536
Val Glu Ser Ala Val Ala Trp Lys Tyr Gly Val Glu Arg Gln Asp Gly           
            500                 505                 510                   

ccg acc gca ctg atc ctc tcc cgt cag aac ctg gcg cag cag gaa cga       1584
Pro Thr Ala Leu Ile Leu Ser Arg Gln Asn Leu Ala Gln Gln Glu Arg           
        515                 520                 525                       

act gaa gag caa ctg gca aac atc gcg cgc ggt ggt tat gtg ctg aaa       1632
Thr Glu Glu Gln Leu Ala Asn Ile Ala Arg Gly Gly Tyr Val Leu Lys           
    530                 535                 540                           

gac tgc gcc ggt cag ccg gaa ctg att ttc atc gct acc ggt tca gaa       1680
Asp Cys Ala Gly Gln Pro Glu Leu Ile Phe Ile Ala Thr Gly Ser Glu           
545                 550                 555                 560           

gtt gaa ctg gct gtt gct gcc tac gaa aaa ctg act gcc gaa ggc gtg       1728
Val Glu Leu Ala Val Ala Ala Tyr Glu Lys Leu Thr Ala Glu Gly Val           
                565                 570                 575               

aaa gcg cgc gtg gtg tcc atg ccg tct acc gac gca ttt gac aag cag       1776
Lys Ala Arg Val Val Ser Met Pro Ser Thr Asp Ala Phe Asp Lys Gln           
            580                 585                 590                   

gat gct gct tac cgt gaa tcc gta ctg ccg aaa gcg gtt act gca cgc       1824
Asp Ala Ala Tyr Arg Glu Ser Val Leu Pro Lys Ala Val Thr Ala Arg           
        595                 600                 605                       

gtt gct gta gaa gcg ggt att gct gac tac tgg tac aag tat gtt ggc       1872
Val Ala Val Glu Ala Gly Ile Ala Asp Tyr Trp Tyr Lys Tyr Val Gly           
    610                 615                 620                           

ctg aac ggt gct atc gtc ggt atg acc acc ttc ggt gaa tct gct ccg       1920
Leu Asn Gly Ala Ile Val Gly Met Thr Thr Phe Gly Glu Ser Ala Pro           
625                 630                 635                 640           

gca gag ctg ctg ttt gaa gag ttc ggc ttc act gtt gat aac gtt gtt       1968
Ala Glu Leu Leu Phe Glu Glu Phe Gly Phe Thr Val Asp Asn Val Val           
                645                 650                 655               

gcg aaa gca aaa gaa ctg ctg taa                                       1992
Ala Lys Ala Lys Glu Leu Leu                                               
            660                                                           


<210>  19
<211>  663
<212>  PRT
<213>  Escherichia coli

<400>  19

Met Ser Ser Arg Lys Glu Leu Ala Asn Ala Ile Arg Ala Leu Ser Met 
1               5                   10                  15      


Asp Ala Val Gln Lys Ala Lys Ser Gly His Pro Gly Ala Pro Met Gly 
            20                  25                  30          


Met Ala Asp Ile Ala Glu Val Leu Trp Arg Asp Phe Leu Lys His Asn 
        35                  40                  45              


Pro Gln Asn Pro Ser Trp Ala Asp Arg Asp Arg Phe Val Leu Ser Asn 
    50                  55                  60                  


Gly His Gly Ser Met Leu Ile Tyr Ser Leu Leu His Leu Thr Gly Tyr 
65                  70                  75                  80  


Asp Leu Pro Met Glu Glu Leu Lys Asn Phe Arg Gln Leu His Ser Lys 
                85                  90                  95      


Thr Pro Gly His Pro Glu Val Gly Tyr Thr Ala Gly Val Glu Thr Thr 
            100                 105                 110         


Thr Gly Pro Leu Gly Gln Gly Ile Ala Asn Ala Val Gly Met Ala Ile 
        115                 120                 125             


Ala Glu Lys Thr Leu Ala Ala Gln Phe Asn Arg Pro Gly His Asp Ile 
    130                 135                 140                 


Val Asp His Tyr Thr Tyr Ala Phe Met Gly Asp Gly Cys Met Met Glu 
145                 150                 155                 160 


Gly Ile Ser His Glu Val Cys Ser Leu Ala Gly Thr Leu Lys Leu Gly 
                165                 170                 175     


Lys Leu Ile Ala Phe Tyr Asp Asp Asn Gly Ile Ser Ile Asp Gly His 
            180                 185                 190         


Val Glu Gly Trp Phe Thr Asp Asp Thr Ala Met Arg Phe Glu Ala Tyr 
        195                 200                 205             


Gly Trp His Val Ile Arg Asp Ile Asp Gly His Asp Ala Ala Ser Ile 
    210                 215                 220                 


Lys Arg Ala Val Glu Glu Ala Arg Ala Val Thr Asp Lys Pro Ser Leu 
225                 230                 235                 240 


Leu Met Cys Lys Thr Ile Ile Gly Phe Gly Ser Pro Asn Lys Ala Gly 
                245                 250                 255     


Thr His Asp Ser His Gly Ala Pro Leu Gly Asp Ala Glu Ile Ala Leu 
            260                 265                 270         


Thr Arg Glu Gln Leu Gly Trp Lys Tyr Ala Pro Phe Glu Ile Pro Ser 
        275                 280                 285             


Glu Ile Tyr Ala Gln Trp Asp Ala Lys Glu Ala Gly Gln Ala Lys Glu 
    290                 295                 300                 


Ser Ala Trp Asn Glu Lys Phe Ala Ala Tyr Ala Lys Ala Tyr Pro Gln 
305                 310                 315                 320 


Glu Ala Ala Glu Phe Thr Arg Arg Met Lys Gly Glu Met Pro Ser Asp 
                325                 330                 335     


Phe Asp Ala Lys Ala Lys Glu Phe Ile Ala Lys Leu Gln Ala Asn Pro 
            340                 345                 350         


Ala Lys Ile Ala Ser Arg Lys Ala Ser Gln Asn Ala Ile Glu Ala Phe 
        355                 360                 365             


Gly Pro Leu Leu Pro Glu Phe Leu Gly Gly Ser Ala Asp Leu Ala Pro 
    370                 375                 380                 


Ser Asn Leu Thr Leu Trp Ser Gly Ser Lys Ala Ile Asn Glu Asp Ala 
385                 390                 395                 400 


Ala Gly Asn Tyr Ile His Tyr Gly Val Arg Glu Phe Gly Met Thr Ala 
                405                 410                 415     


Ile Ala Asn Gly Ile Ser Leu His Gly Gly Phe Leu Pro Tyr Thr Ser 
            420                 425                 430         


Thr Phe Leu Met Phe Val Glu Tyr Ala Arg Asn Ala Val Arg Met Ala 
        435                 440                 445             


Ala Leu Met Lys Gln Arg Gln Val Met Val Tyr Thr His Asp Ser Ile 
    450                 455                 460                 


Gly Leu Gly Glu Asp Gly Pro Thr His Gln Pro Val Glu Gln Val Ala 
465                 470                 475                 480 


Ser Leu Arg Val Thr Pro Asn Met Ser Thr Trp Arg Pro Cys Asp Gln 
                485                 490                 495     


Val Glu Ser Ala Val Ala Trp Lys Tyr Gly Val Glu Arg Gln Asp Gly 
            500                 505                 510         


Pro Thr Ala Leu Ile Leu Ser Arg Gln Asn Leu Ala Gln Gln Glu Arg 
        515                 520                 525             


Thr Glu Glu Gln Leu Ala Asn Ile Ala Arg Gly Gly Tyr Val Leu Lys 
    530                 535                 540                 


Asp Cys Ala Gly Gln Pro Glu Leu Ile Phe Ile Ala Thr Gly Ser Glu 
545                 550                 555                 560 


Val Glu Leu Ala Val Ala Ala Tyr Glu Lys Leu Thr Ala Glu Gly Val 
                565                 570                 575     


Lys Ala Arg Val Val Ser Met Pro Ser Thr Asp Ala Phe Asp Lys Gln 
            580                 585                 590         


Asp Ala Ala Tyr Arg Glu Ser Val Leu Pro Lys Ala Val Thr Ala Arg 
        595                 600                 605             


Val Ala Val Glu Ala Gly Ile Ala Asp Tyr Trp Tyr Lys Tyr Val Gly 
    610                 615                 620                 


Leu Asn Gly Ala Ile Val Gly Met Thr Thr Phe Gly Glu Ser Ala Pro 
625                 630                 635                 640 


Ala Glu Leu Leu Phe Glu Glu Phe Gly Phe Thr Val Asp Asn Val Val 
                645                 650                 655     


Ala Lys Ala Lys Glu Leu Leu 
            660             


<210>  20
<211>  1080
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(1080)

<400>  20
atg tct aag att ttt gat ttc gta aaa cct ggc gta atc act ggt gat         48
Met Ser Lys Ile Phe Asp Phe Val Lys Pro Gly Val Ile Thr Gly Asp           
1               5                   10                  15                

gac gta cag aaa gtt ttc cag gta gca aaa gaa aac aac ttc gca ctg         96
Asp Val Gln Lys Val Phe Gln Val Ala Lys Glu Asn Asn Phe Ala Leu           
            20                  25                  30                    

cca gca gta aac tgc gtc ggt act gac tcc atc aac gcc gta ctg gaa        144
Pro Ala Val Asn Cys Val Gly Thr Asp Ser Ile Asn Ala Val Leu Glu           
        35                  40                  45                        

acc gct gct aaa gtt aaa gcg ccg gtt atc gtt cag ttc tcc aac ggt        192
Thr Ala Ala Lys Val Lys Ala Pro Val Ile Val Gln Phe Ser Asn Gly           
    50                  55                  60                            

ggt gct tcc ttt atc gct ggt aaa ggc gtg aaa tct gac gtt ccg cag        240
Gly Ala Ser Phe Ile Ala Gly Lys Gly Val Lys Ser Asp Val Pro Gln           
65                  70                  75                  80            

ggt gct gct atc ctg ggc gcg atc tct ggt gcg cat cac gtt cac cag        288
Gly Ala Ala Ile Leu Gly Ala Ile Ser Gly Ala His His Val His Gln           
                85                  90                  95                

atg gct gaa cat tat ggt gtt ccg gtt atc ctg cac act gac cac tgc        336
Met Ala Glu His Tyr Gly Val Pro Val Ile Leu His Thr Asp His Cys           
            100                 105                 110                   

gcg aag aaa ctg ctg ccg tgg atc gac ggt ctg ttg gac gcg ggt gaa        384
Ala Lys Lys Leu Leu Pro Trp Ile Asp Gly Leu Leu Asp Ala Gly Glu           
        115                 120                 125                       

aaa cac ttc gca gct acc ggt aag ccg ctg ttc tct tct cac atg atc        432
Lys His Phe Ala Ala Thr Gly Lys Pro Leu Phe Ser Ser His Met Ile           
    130                 135                 140                           

gac ctg tct gaa gaa tct ctg caa gag aac atc gaa atc tgc tct aaa        480
Asp Leu Ser Glu Glu Ser Leu Gln Glu Asn Ile Glu Ile Cys Ser Lys           
145                 150                 155                 160           

tac ctg gag cgc atg tcc aaa atc ggc atg act ctg gaa atc gaa ctg        528
Tyr Leu Glu Arg Met Ser Lys Ile Gly Met Thr Leu Glu Ile Glu Leu           
                165                 170                 175               

ggt tgc acc ggt ggt gaa gaa gac ggc gtg gac aac agc cac atg gac        576
Gly Cys Thr Gly Gly Glu Glu Asp Gly Val Asp Asn Ser His Met Asp           
            180                 185                 190                   

gct tct gca ctg tac acc cag ccg gaa gac gtt gat tac gca tac acc        624
Ala Ser Ala Leu Tyr Thr Gln Pro Glu Asp Val Asp Tyr Ala Tyr Thr           
        195                 200                 205                       

gaa ctg agc aaa atc agc ccg cgt ttc acc atc gca gcg tcc ttc ggt        672
Glu Leu Ser Lys Ile Ser Pro Arg Phe Thr Ile Ala Ala Ser Phe Gly           
    210                 215                 220                           

aac gta cac ggt gtt tac aag ccg ggt aac gtg gtt ctg act ccg acc        720
Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Val Leu Thr Pro Thr           
225                 230                 235                 240           

atc ctg cgt gat tct cag gaa tat gtt tcc aag aaa cac aac ctg ccg        768
Ile Leu Arg Asp Ser Gln Glu Tyr Val Ser Lys Lys His Asn Leu Pro           
                245                 250                 255               

cac aac agc ctg aac ttc gta ttc cac ggt ggt tcc ggt tct act gct        816
His Asn Ser Leu Asn Phe Val Phe His Gly Gly Ser Gly Ser Thr Ala           
            260                 265                 270                   

cag gaa atc aaa gac tcc gta agc tac ggc gta gta aaa atg aac atc        864
Gln Glu Ile Lys Asp Ser Val Ser Tyr Gly Val Val Lys Met Asn Ile           
        275                 280                 285                       

gat acc gat acc caa tgg gca acc tgg gaa ggc gtt ctg aac tac tac        912
Asp Thr Asp Thr Gln Trp Ala Thr Trp Glu Gly Val Leu Asn Tyr Tyr           
    290                 295                 300                           

aaa gcg aac gaa gct tat ctg cag ggt cag ctg ggt aac ccg aaa ggc        960
Lys Ala Asn Glu Ala Tyr Leu Gln Gly Gln Leu Gly Asn Pro Lys Gly           
305                 310                 315                 320           

gaa gat cag ccg aac aag aaa tac tac gat ccg cgc gta tgg ctg cgt       1008
Glu Asp Gln Pro Asn Lys Lys Tyr Tyr Asp Pro Arg Val Trp Leu Arg           
                325                 330                 335               

gcc ggt cag act tcg atg atc gct cgt ctg gag aaa gca ttc cag gaa       1056
Ala Gly Gln Thr Ser Met Ile Ala Arg Leu Glu Lys Ala Phe Gln Glu           
            340                 345                 350                   

ctg aac gcg atc gac gtt ctg taa                                       1080
Leu Asn Ala Ile Asp Val Leu                                               
        355                                                               


<210>  21
<211>  359
<212>  PRT
<213>  Escherichia coli

<400>  21

Met Ser Lys Ile Phe Asp Phe Val Lys Pro Gly Val Ile Thr Gly Asp 
1               5                   10                  15      


Asp Val Gln Lys Val Phe Gln Val Ala Lys Glu Asn Asn Phe Ala Leu 
            20                  25                  30          


Pro Ala Val Asn Cys Val Gly Thr Asp Ser Ile Asn Ala Val Leu Glu 
        35                  40                  45              


Thr Ala Ala Lys Val Lys Ala Pro Val Ile Val Gln Phe Ser Asn Gly 
    50                  55                  60                  


Gly Ala Ser Phe Ile Ala Gly Lys Gly Val Lys Ser Asp Val Pro Gln 
65                  70                  75                  80  


Gly Ala Ala Ile Leu Gly Ala Ile Ser Gly Ala His His Val His Gln 
                85                  90                  95      


Met Ala Glu His Tyr Gly Val Pro Val Ile Leu His Thr Asp His Cys 
            100                 105                 110         


Ala Lys Lys Leu Leu Pro Trp Ile Asp Gly Leu Leu Asp Ala Gly Glu 
        115                 120                 125             


Lys His Phe Ala Ala Thr Gly Lys Pro Leu Phe Ser Ser His Met Ile 
    130                 135                 140                 


Asp Leu Ser Glu Glu Ser Leu Gln Glu Asn Ile Glu Ile Cys Ser Lys 
145                 150                 155                 160 


Tyr Leu Glu Arg Met Ser Lys Ile Gly Met Thr Leu Glu Ile Glu Leu 
                165                 170                 175     


Gly Cys Thr Gly Gly Glu Glu Asp Gly Val Asp Asn Ser His Met Asp 
            180                 185                 190         


Ala Ser Ala Leu Tyr Thr Gln Pro Glu Asp Val Asp Tyr Ala Tyr Thr 
        195                 200                 205             


Glu Leu Ser Lys Ile Ser Pro Arg Phe Thr Ile Ala Ala Ser Phe Gly 
    210                 215                 220                 


Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Val Leu Thr Pro Thr 
225                 230                 235                 240 


Ile Leu Arg Asp Ser Gln Glu Tyr Val Ser Lys Lys His Asn Leu Pro 
                245                 250                 255     


His Asn Ser Leu Asn Phe Val Phe His Gly Gly Ser Gly Ser Thr Ala 
            260                 265                 270         


Gln Glu Ile Lys Asp Ser Val Ser Tyr Gly Val Val Lys Met Asn Ile 
        275                 280                 285             


Asp Thr Asp Thr Gln Trp Ala Thr Trp Glu Gly Val Leu Asn Tyr Tyr 
    290                 295                 300                 


Lys Ala Asn Glu Ala Tyr Leu Gln Gly Gln Leu Gly Asn Pro Lys Gly 
305                 310                 315                 320 


Glu Asp Gln Pro Asn Lys Lys Tyr Tyr Asp Pro Arg Val Trp Leu Arg 
                325                 330                 335     


Ala Gly Gln Thr Ser Met Ile Ala Arg Leu Glu Lys Ala Phe Gln Glu 
            340                 345                 350         


Leu Asn Ala Ile Asp Val Leu 
        355                 


<210>  22
<211>  394
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  22

Met Asn Lys Lys Thr Ile Arg Asp Val Asp Val Arg Gly Lys Arg Val 
1               5                   10                  15      


Phe Cys Arg Val Asp Phe Asn Val Pro Met Glu Gln Gly Ala Ile Thr 
            20                  25                  30          


Asp Asp Thr Arg Ile Arg Ala Ala Leu Pro Thr Ile Arg Tyr Leu Ile 
        35                  40                  45              


Glu His Gly Ala Lys Val Ile Leu Ala Ser His Leu Gly Arg Pro Lys 
    50                  55                  60                  


Gly Lys Val Val Glu Glu Leu Arg Leu Asp Ala Val Ala Lys Arg Leu 
65                  70                  75                  80  


Gly Glu Leu Leu Glu Arg Pro Val Ala Lys Thr Asn Glu Ala Val Gly 
                85                  90                  95      


Asp Glu Val Lys Ala Ala Val Asp Arg Leu Asn Glu Gly Asp Val Leu 
            100                 105                 110         


Leu Leu Glu Asn Val Arg Phe Tyr Pro Gly Glu Glu Lys Asn Asp Pro 
        115                 120                 125             


Glu Leu Ala Lys Ala Phe Ala Glu Leu Ala Asp Leu Tyr Val Asn Asp 
    130                 135                 140                 


Ala Phe Gly Ala Ala His Arg Ala His Ala Ser Thr Glu Gly Ile Ala 
145                 150                 155                 160 


His Tyr Leu Pro Ala Val Ala Gly Phe Leu Met Glu Lys Glu Leu Glu 
                165                 170                 175     


Val Leu Gly Lys Ala Leu Ser Asn Pro Asp Arg Pro Phe Thr Ala Ile 
            180                 185                 190         


Ile Gly Gly Ala Lys Val Lys Asp Lys Ile Gly Val Ile Asp Asn Leu 
        195                 200                 205             


Leu Glu Lys Val Asp Asn Leu Ile Ile Gly Gly Gly Leu Ala Tyr Thr 
    210                 215                 220                 


Phe Val Lys Ala Leu Gly His Asp Val Gly Lys Ser Leu Leu Glu Glu 
225                 230                 235                 240 


Asp Lys Ile Glu Leu Ala Lys Ser Phe Met Glu Lys Ala Lys Glu Lys 
                245                 250                 255     


Gly Val Arg Phe Tyr Met Pro Val Asp Val Val Val Ala Asp Arg Phe 
            260                 265                 270         


Ala Asn Asp Ala Asn Thr Lys Val Val Pro Ile Asp Ala Ile Pro Ala 
        275                 280                 285             


Asp Trp Ser Ala Leu Asp Ile Gly Pro Lys Thr Arg Glu Leu Tyr Arg 
    290                 295                 300                 


Asp Val Ile Arg Glu Ser Lys Leu Val Val Trp Asn Gly Pro Met Gly 
305                 310                 315                 320 


Val Phe Glu Met Asp Ala Phe Ala His Gly Thr Lys Ala Ile Ala Glu 
                325                 330                 335     


Ala Leu Ala Glu Ala Leu Asp Thr Tyr Ser Val Ile Gly Gly Gly Asp 
            340                 345                 350         


Ser Ala Ala Ala Val Glu Lys Phe Gly Leu Ala Asp Lys Met Asp His 
        355                 360                 365             


Ile Ser Thr Gly Gly Gly Ala Ser Leu Glu Phe Met Glu Gly Lys Gln 
    370                 375                 380                 


Leu Pro Gly Val Val Ala Leu Glu Asp Lys 
385                 390                 


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rpiA knockout forward primer

<400>  23
cgccttctac cagcagaaac                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rpiA knockout reverse primer

<400>  24
cccagaccgt tgtatgcttt                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rpiB knockout forward primer

<400>  25
ggaagcgctg aatcaaactc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rpiB knockout reverse primer

<400>  26
gctcttcatc ctccagttgc                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nupG knockin forward primer

<400>  27
atatgccatt tgccacacca                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nupG knockin reverse primer

<400>  28
cttatattcg cggtgacgtg                                                   20


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS3 knockin forward primer

<400>  29
tgttaattag cgggcaattg tacc                                              24


<210>  30
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SS3 knockin reverse primer

<400>  30
gatacctaca gcgcagaaaa acaa                                              24


<210>  31
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gapA knockout/gapC knockin forward primer

<400>  31
tgcttcgata ttatggcggg ctt                                               23


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gapA knockout/gapC knockin reverse primer

<400>  32
gccagatgtg caggtttctc ttt                                               23


<210>  33
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pfkA knckout forward primer

<400>  33
atcaatctta tggacggctg gtc                                               23


<210>  34
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pfkA knockout reverse primer

<400>  34
tgctgatctg atcgaacgta ccg                                               23


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  frmA frameshift validation forward primer

<400>  35
tattttgcca gccgccaaag                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  frmA frameshift validation reverse primer

<400>  36
cgaaatgact gctacagccg                                                   20


<210>  37
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pgi deletion validation forward primer

<400>  37
gaagtcaacg cggtgctg                                                     18


<210>  38
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pgi deletion validation reverse primer

<400>  38
ccctggtgga tcagctgg                                                     18


<210>  39
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFC139 plasmid variation validation forward primer

<400>  39
atcctactgc ttttttcaat tcatc                                             25


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFC139 plasmid variation validation reverse primer

<400>  40
caagggtgaa cactatccca                                                   20


<210>  41
<211>  963
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(963)

<400>  41
atg att aag aaa atc ggt gtg ttg aca agc ggc ggt gat gcg cca ggc         48
Met Ile Lys Lys Ile Gly Val Leu Thr Ser Gly Gly Asp Ala Pro Gly           
1               5                   10                  15                

atg aac gcc gca att cgc ggg gtt gtt cgt tct gcg ctg aca gaa ggt         96
Met Asn Ala Ala Ile Arg Gly Val Val Arg Ser Ala Leu Thr Glu Gly           
            20                  25                  30                    

ctg gaa gta atg ggt att tat gac ggc tat ctg ggt ctg tat gaa gac        144
Leu Glu Val Met Gly Ile Tyr Asp Gly Tyr Leu Gly Leu Tyr Glu Asp           
        35                  40                  45                        

cgt atg gta cag cta gac cgt tac agc gtg tct gac atg atc aac cgt        192
Arg Met Val Gln Leu Asp Arg Tyr Ser Val Ser Asp Met Ile Asn Arg           
    50                  55                  60                            

ggc ggt acg ttc ctc ggt tct gcg cgt ttc ccg gaa ttc cgc gac gag        240
Gly Gly Thr Phe Leu Gly Ser Ala Arg Phe Pro Glu Phe Arg Asp Glu           
65                  70                  75                  80            

aac atc cgc gcc gtg gct atc gaa aac ctg aaa aaa cgt ggt atc gac        288
Asn Ile Arg Ala Val Ala Ile Glu Asn Leu Lys Lys Arg Gly Ile Asp           
                85                  90                  95                

gcg ctg gtg gtt atc ggc ggt gac ggt tcc tac atg ggt gca atg cgt        336
Ala Leu Val Val Ile Gly Gly Asp Gly Ser Tyr Met Gly Ala Met Arg           
            100                 105                 110                   

ctg acc gaa atg ggc ttc ccg tgc atc ggt ctg ccg ggc act atc gac        384
Leu Thr Glu Met Gly Phe Pro Cys Ile Gly Leu Pro Gly Thr Ile Asp           
        115                 120                 125                       

aac gac atc aaa ggc act gac tac act atc ggt ttc ttc act gcg ctg        432
Asn Asp Ile Lys Gly Thr Asp Tyr Thr Ile Gly Phe Phe Thr Ala Leu           
    130                 135                 140                           

agc acc gtt gta gaa gcg atc gac cgt ctg cgt gac acc tct tct tct        480
Ser Thr Val Val Glu Ala Ile Asp Arg Leu Arg Asp Thr Ser Ser Ser           
145                 150                 155                 160           

cac cag cgt att tcc gtg gtg gaa gtg atg ggc cgt tat tgt gga gat        528
His Gln Arg Ile Ser Val Val Glu Val Met Gly Arg Tyr Cys Gly Asp           
                165                 170                 175               

ctg acg ttg gct gcg gcc att gcc ggt ggc tgt gaa ttc gtt gtg gtt        576
Leu Thr Leu Ala Ala Ala Ile Ala Gly Gly Cys Glu Phe Val Val Val           
            180                 185                 190                   

ccg gaa gtt gaa ttc agc cgt gaa gac ctg gta aac gaa atc aaa gcg        624
Pro Glu Val Glu Phe Ser Arg Glu Asp Leu Val Asn Glu Ile Lys Ala           
        195                 200                 205                       

ggt atc gcg aaa ggt aaa aaa cac gcg atc gtg gcg att acc gaa cat        672
Gly Ile Ala Lys Gly Lys Lys His Ala Ile Val Ala Ile Thr Glu His           
    210                 215                 220                           

atg tgt gat gtt gac gaa ctg gcg cat ttc atc gag aaa gaa acc ggt        720
Met Cys Asp Val Asp Glu Leu Ala His Phe Ile Glu Lys Glu Thr Gly           
225                 230                 235                 240           

cgt gaa acc cgc gca act gtg ctg ggc cac atc cag cgc ggt ggt tct        768
Arg Glu Thr Arg Ala Thr Val Leu Gly His Ile Gln Arg Gly Gly Ser           
                245                 250                 255               

ccg gtg cct tac gac cgt att ctg gct tcc cgt atg ggc gct tac gct        816
Pro Val Pro Tyr Asp Arg Ile Leu Ala Ser Arg Met Gly Ala Tyr Ala           
            260                 265                 270                   

atc gat ctg ctg ctg gca ggt tac ggc ggt cgt tgt gta ggt atc cag        864
Ile Asp Leu Leu Leu Ala Gly Tyr Gly Gly Arg Cys Val Gly Ile Gln           
        275                 280                 285                       

aac gaa cag ctg gtt cac cac gac atc atc gac gct atc gaa aac atg        912
Asn Glu Gln Leu Val His His Asp Ile Ile Asp Ala Ile Glu Asn Met           
    290                 295                 300                           

aag cgt ccg ttc aaa ggt gac tgg ctg gac tgc gcg aaa aaa ctg tat        960
Lys Arg Pro Phe Lys Gly Asp Trp Leu Asp Cys Ala Lys Lys Leu Tyr           
305                 310                 315                 320           

taa                                                                    963


<210>  42
<211>  320
<212>  PRT
<213>  Escherichia coli

<400>  42

Met Ile Lys Lys Ile Gly Val Leu Thr Ser Gly Gly Asp Ala Pro Gly 
1               5                   10                  15      


Met Asn Ala Ala Ile Arg Gly Val Val Arg Ser Ala Leu Thr Glu Gly 
            20                  25                  30          


Leu Glu Val Met Gly Ile Tyr Asp Gly Tyr Leu Gly Leu Tyr Glu Asp 
        35                  40                  45              


Arg Met Val Gln Leu Asp Arg Tyr Ser Val Ser Asp Met Ile Asn Arg 
    50                  55                  60                  


Gly Gly Thr Phe Leu Gly Ser Ala Arg Phe Pro Glu Phe Arg Asp Glu 
65                  70                  75                  80  


Asn Ile Arg Ala Val Ala Ile Glu Asn Leu Lys Lys Arg Gly Ile Asp 
                85                  90                  95      


Ala Leu Val Val Ile Gly Gly Asp Gly Ser Tyr Met Gly Ala Met Arg 
            100                 105                 110         


Leu Thr Glu Met Gly Phe Pro Cys Ile Gly Leu Pro Gly Thr Ile Asp 
        115                 120                 125             


Asn Asp Ile Lys Gly Thr Asp Tyr Thr Ile Gly Phe Phe Thr Ala Leu 
    130                 135                 140                 


Ser Thr Val Val Glu Ala Ile Asp Arg Leu Arg Asp Thr Ser Ser Ser 
145                 150                 155                 160 


His Gln Arg Ile Ser Val Val Glu Val Met Gly Arg Tyr Cys Gly Asp 
                165                 170                 175     


Leu Thr Leu Ala Ala Ala Ile Ala Gly Gly Cys Glu Phe Val Val Val 
            180                 185                 190         


Pro Glu Val Glu Phe Ser Arg Glu Asp Leu Val Asn Glu Ile Lys Ala 
        195                 200                 205             


Gly Ile Ala Lys Gly Lys Lys His Ala Ile Val Ala Ile Thr Glu His 
    210                 215                 220                 


Met Cys Asp Val Asp Glu Leu Ala His Phe Ile Glu Lys Glu Thr Gly 
225                 230                 235                 240 


Arg Glu Thr Arg Ala Thr Val Leu Gly His Ile Gln Arg Gly Gly Ser 
                245                 250                 255     


Pro Val Pro Tyr Asp Arg Ile Leu Ala Ser Arg Met Gly Ala Tyr Ala 
            260                 265                 270         


Ile Asp Leu Leu Leu Ala Gly Tyr Gly Gly Arg Cys Val Gly Ile Gln 
        275                 280                 285             


Asn Glu Gln Leu Val His His Asp Ile Ile Asp Ala Ile Glu Asn Met 
    290                 295                 300                 


Lys Arg Pro Phe Lys Gly Asp Trp Leu Asp Cys Ala Lys Lys Leu Tyr 
305                 310                 315                 320 


<210>  43
<211>  930
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(930)

<400>  43
atg gta cgt atc tat acg ttg aca ctt gcg ccc tct ctc gat agc gca         48
Met Val Arg Ile Tyr Thr Leu Thr Leu Ala Pro Ser Leu Asp Ser Ala           
1               5                   10                  15                

aca att acc ccg caa att tat ccc gaa gga aaa ctg cgc tgt acc gca         96
Thr Ile Thr Pro Gln Ile Tyr Pro Glu Gly Lys Leu Arg Cys Thr Ala           
            20                  25                  30                    

ccg gtg ttc gaa ccc ggg ggc ggc ggc atc aac gtc gcc cgc gcc att        144
Pro Val Phe Glu Pro Gly Gly Gly Gly Ile Asn Val Ala Arg Ala Ile           
        35                  40                  45                        

gcc cat ctt gga ggc agt gcc aca gcg atc ttc ccg gcg ggt ggc gcg        192
Ala His Leu Gly Gly Ser Ala Thr Ala Ile Phe Pro Ala Gly Gly Ala           
    50                  55                  60                            

acc ggc gaa cac ctg gtt tca ctg ttg gcg gat gaa aat gtc ccc gtc        240
Thr Gly Glu His Leu Val Ser Leu Leu Ala Asp Glu Asn Val Pro Val           
65                  70                  75                  80            

gct act gta gaa gcc aaa gac tgg acc cgg cag aat tta cac gta cat        288
Ala Thr Val Glu Ala Lys Asp Trp Thr Arg Gln Asn Leu His Val His           
                85                  90                  95                

gtg gaa gca agc ggt gag cag tat cgt ttt gtt atg cca ggc gcg gca        336
Val Glu Ala Ser Gly Glu Gln Tyr Arg Phe Val Met Pro Gly Ala Ala           
            100                 105                 110                   

tta aat gaa gat gag ttt cgc cag ctt gaa gag caa gtt ctg gaa att        384
Leu Asn Glu Asp Glu Phe Arg Gln Leu Glu Glu Gln Val Leu Glu Ile           
        115                 120                 125                       

gaa tcc ggg gcc atc ctg gtc ata agc gga agc ctg ccg cca ggt gtg        432
Glu Ser Gly Ala Ile Leu Val Ile Ser Gly Ser Leu Pro Pro Gly Val           
    130                 135                 140                           

aag ctg gaa aaa tta acc caa ctg att tcc gct gcg caa aaa caa ggg        480
Lys Leu Glu Lys Leu Thr Gln Leu Ile Ser Ala Ala Gln Lys Gln Gly           
145                 150                 155                 160           

atc cgc tgc atc gtc gac agt tct ggc gaa gcg tta agt gca gca ctg        528
Ile Arg Cys Ile Val Asp Ser Ser Gly Glu Ala Leu Ser Ala Ala Leu           
                165                 170                 175               

gca att ggt aac atc gag ttg gtt aag cct aac caa aaa gaa ctc agt        576
Ala Ile Gly Asn Ile Glu Leu Val Lys Pro Asn Gln Lys Glu Leu Ser           
            180                 185                 190                   

gcg ctg gtg aat cgc gaa ctc acc cag ccg gac gat gtc cgc aaa gcc        624
Ala Leu Val Asn Arg Glu Leu Thr Gln Pro Asp Asp Val Arg Lys Ala           
        195                 200                 205                       

gcg cag gaa atc gtt aat agc ggc aag gcc aaa cgg gtt gtc gtt tcc        672
Ala Gln Glu Ile Val Asn Ser Gly Lys Ala Lys Arg Val Val Val Ser           
    210                 215                 220                           

ctg ggt cca caa gga gcg ctg ggt gtt gat agt gaa aac tgt att cag        720
Leu Gly Pro Gln Gly Ala Leu Gly Val Asp Ser Glu Asn Cys Ile Gln           
225                 230                 235                 240           

gtg gtg cca cca ccg gtg aaa agc cag agt acc gtt ggc gct ggt gac        768
Val Val Pro Pro Pro Val Lys Ser Gln Ser Thr Val Gly Ala Gly Asp           
                245                 250                 255               

agc atg gtc ggc gcg atg aca ctg aaa ctg gca gaa aat gcc tct ctt        816
Ser Met Val Gly Ala Met Thr Leu Lys Leu Ala Glu Asn Ala Ser Leu           
            260                 265                 270                   

gaa gag atg gtt cgt ttt ggc gta gct gcg ggg agt gca gcc aca ctc        864
Glu Glu Met Val Arg Phe Gly Val Ala Ala Gly Ser Ala Ala Thr Leu           
        275                 280                 285                       

aat cag gga aca cgt ctg tgc tcc cat gac gat acg caa aaa att tac        912
Asn Gln Gly Thr Arg Leu Cys Ser His Asp Asp Thr Gln Lys Ile Tyr           
    290                 295                 300                           

gct tac ctt tcc cgc taa                                                930
Ala Tyr Leu Ser Arg                                                       
305                                                                       


<210>  44
<211>  309
<212>  PRT
<213>  Escherichia coli

<400>  44

Met Val Arg Ile Tyr Thr Leu Thr Leu Ala Pro Ser Leu Asp Ser Ala 
1               5                   10                  15      


Thr Ile Thr Pro Gln Ile Tyr Pro Glu Gly Lys Leu Arg Cys Thr Ala 
            20                  25                  30          


Pro Val Phe Glu Pro Gly Gly Gly Gly Ile Asn Val Ala Arg Ala Ile 
        35                  40                  45              


Ala His Leu Gly Gly Ser Ala Thr Ala Ile Phe Pro Ala Gly Gly Ala 
    50                  55                  60                  


Thr Gly Glu His Leu Val Ser Leu Leu Ala Asp Glu Asn Val Pro Val 
65                  70                  75                  80  


Ala Thr Val Glu Ala Lys Asp Trp Thr Arg Gln Asn Leu His Val His 
                85                  90                  95      


Val Glu Ala Ser Gly Glu Gln Tyr Arg Phe Val Met Pro Gly Ala Ala 
            100                 105                 110         


Leu Asn Glu Asp Glu Phe Arg Gln Leu Glu Glu Gln Val Leu Glu Ile 
        115                 120                 125             


Glu Ser Gly Ala Ile Leu Val Ile Ser Gly Ser Leu Pro Pro Gly Val 
    130                 135                 140                 


Lys Leu Glu Lys Leu Thr Gln Leu Ile Ser Ala Ala Gln Lys Gln Gly 
145                 150                 155                 160 


Ile Arg Cys Ile Val Asp Ser Ser Gly Glu Ala Leu Ser Ala Ala Leu 
                165                 170                 175     


Ala Ile Gly Asn Ile Glu Leu Val Lys Pro Asn Gln Lys Glu Leu Ser 
            180                 185                 190         


Ala Leu Val Asn Arg Glu Leu Thr Gln Pro Asp Asp Val Arg Lys Ala 
        195                 200                 205             


Ala Gln Glu Ile Val Asn Ser Gly Lys Ala Lys Arg Val Val Val Ser 
    210                 215                 220                 


Leu Gly Pro Gln Gly Ala Leu Gly Val Asp Ser Glu Asn Cys Ile Gln 
225                 230                 235                 240 


Val Val Pro Pro Pro Val Lys Ser Gln Ser Thr Val Gly Ala Gly Asp 
                245                 250                 255     


Ser Met Val Gly Ala Met Thr Leu Lys Leu Ala Glu Asn Ala Ser Leu 
            260                 265                 270         


Glu Glu Met Val Arg Phe Gly Val Ala Ala Gly Ser Ala Ala Thr Leu 
        275                 280                 285             


Asn Gln Gly Thr Arg Leu Cys Ser His Asp Asp Thr Gln Lys Ile Tyr 
    290                 295                 300                 


Ala Tyr Leu Ser Arg 
305                 


<210>  45
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBS sequence

<400>  45
gactaaaaac attcggaggc ttaagcagtc atcgt                                  35


