                         SEQUENCE LISTING

<110>  Optimvia LLC
       Ferreira Gesteira, Tarsis
       Lajiness, Daniel H.
 
<120>  METHODS FOR SYNTHESIZING NON-ANTICOAGULANT HEPARAN SULFATE

<130>  OPT-008P

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 1

<400>  1
atgagcgaag agaaggaccc tttgtggcag gacccgtgcg aggacaaacg ccacaaagac       60

atttggtcga aggagaaaac ctgtgaccgc ttccctaagt tgcttattat tggtccgcaa      120

aagaccggcg cctgggcgct ttaccatttc ctgggtatgc atcccgatct tagttccaac      180

tacccgtcga gtgaaacaca tggcagtatc caattcttta atggacataa ctaccataag      240

ggcatcgact ggtatatgga atttttcccc attccctcaa ataccacttc tgacttttat      300

ttcgagaaat cagcgaatta ttttgacagt gaggtagcgc ctcgccgcgc agcagcattg      360

ttgcccaaag caaaagtgct gactattctt atcaatccag ctgaccgcgc atattcttgg      420

tatcagcacc agcgcgccca cgacgacccg gtggcgctga aatacacatt ccatgaagtg      480

attactgctg gaagcgatgc gtcgtctaag ttgcgtgctc tgcagaaccg ctgtttggta      540

cctggctggt atgctacgca cattgaacgt tggctgtccg catatcacgc gaaccagatc      600

ctggttttag atggtaaatt acttcgcacg gagccagcta aagtcatgga catggtacaa      660

aagttcctgg gggtaacgaa taccattgat tatcataaga ctttggcttt cgaccccaag      720

aagggatttt ggtgccagtt attggagggg ggcaagacga agtgcttagg caaatcgcat      780

gggcgcaagt acccggagat ggatttggac tcacgcgcct ttcttaagga ctactaccgc      840

gaccacaaca ttgaattgag taaattatta tacaaaatgg ggcaaactct tccgacttgg      900

ttgcgtgaag acttgcagaa cacacgc                                          927


<210>  2
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 1

<400>  2

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Gln Lys Thr Gly Ala Trp Ala Leu Tyr 
        35                  40                  45              


His Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr His Gly Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Lys Ser Ala Asn Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Leu 
                245                 250                 255     


Gly Lys Ser His Gly Arg Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  3
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 2

<400>  3
atgagcgaag agaaggaccc tttgtggcag gacccgtgcg aggacaaacg ccataaggac       60

atctggtcga aagagaagac ttgtgaccgt tttccaaaat tacttattat cggtccttca      120

aagaccggcg ctttcctttt aacccacttt ttggggatgc atccagacct tagttcaaat      180

tacccttcgt ctgagactgg gcattccatt caattcttca acgggcacaa ttatcacaag      240

ggtattgact ggtacatgga atttttcccg attccgagca atacaacttc cgatttttac      300

tttgaaacct catccaatta ttttgattcc gaagtcgctc cacgccgcgc cgctgctttg      360

ttgccaaaag ctaaggtttt gactattctg atcaacccgg ctgaccgcgc ctattcatgg      420

taccaacacc agcgtgctca tgatgaccca gtggctttga agtatacgtt ccatgaggtc      480

attacagcgg gcagcgacgc aagctccaaa cttcgcgcat tgcaaaaccg ctgccttgtg      540

cccggttggt acgcgacaca cattgaacgc tggctgtccg cttaccacgc caaccaaatt      600

ttagttttag atgggaaatt acttcgtacc gaacctgcca aggtcatgga catggtgcag      660

aaatttttgg gagtcactaa cactatcgac taccacaaaa cattggcatt cgatccaaaa      720

aaggggtttt ggtgccagct tttagaaggg ggcaagacga agtgtcacgg gaagcgttgg      780

gggcgtaagt atccagagat ggatcttgat agccgcgctt tcttaaaaga ttattaccgt      840

gaccacaaca ttgagcttag caaactgctt tacaagatgg gtcagacact tccgacatgg      900

ctgcgtgaag acttgcagaa cacacgc                                          927


<210>  4
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 2

<400>  4

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Ser Lys Thr Gly Ala Phe Leu Leu Thr 
        35                  40                  45              


His Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Gly His Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Thr Ser Ser Asn Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys His 
                245                 250                 255     


Gly Lys Arg Trp Gly Arg Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  5
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 3

<400>  5
atgagcgaag agaaggaccc tttgtggcag gacccgtgcg aagataagcg tcacaaggac       60

atctggtcaa aagagaaaac ttgcgaccgc tttccgaaat tgttaattat tggaccacat      120

ggcaccgggg gtcacgcact ttacttattc ttgggaatgc acccagatct gagctccaac      180

taccccagct ctgaaaccgg cgaagaaatc caatttttca acgggcacaa ttatcataaa      240

ggcattgatt ggtatatgga attcttcccc atcccgtcta atactaccag cgatttctat      300

tttgaaaaaa gtgcgaacta cttcgactcg gaggtggcac cccgtcgtgc tgcggcctta      360

ctgccaaagg ccaaggtttt aaccatcttg attaatccgg ctgaccgtgc ttattcctgg      420

taccaggctc aacgcgcaca tgacgacccc gttgcgctta aatatacatt ccacgaggtc      480

attactgcgg gctctgatgc ttcttcgaaa cttcgtgcgc tgcaaaatcg ttgtttagtg      540

ccgggttggt acgccacgca catcgagcgt tggcttagtg cctaccatgc gaatcaaatc      600

cttgtcttgg atgggaagct tttgcgtact gaaccggcca aggtcatgga catggtccag      660

aagtttctgg gtgttaccaa cactattgat taccataaga ctttagcctt cgatccgaag      720

aaaggcttct ggtgtcaatt acttgagggt ggtaagacca agtgcggagg aaaacatctt      780

gggcgcaaat accccgaaat ggacttagat agccgtgcct ttctgaaaga ttactaccgc      840

gaccataata tcgagcttag caaattattg tacaaaatgg gccaaacctt gccgacgtgg      900

ctgcgtgaag acttgcagaa cacacgc                                          927


<210>  6
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 3

<400>  6

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His Gly Thr Gly Gly His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Gly Glu Glu Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Lys Ser Ala Asn Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln Ala Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Gly 
                245                 250                 255     


Gly Lys His Leu Gly Arg Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  7
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 4

<400>  7
atgagcgaag agaaggaccc tttgtggcag gacccgtgcg aagataagcg ccacaaggac       60

atctggagca aggagaaaac ttgcgaccgc tttccaaagt tgctgattat tgggcctcac      120

ggcacgggcg gccacgcgct gtacctgttt cttggcatgc acccggacct ttccagcaat      180

tatcctagta gtgagacatt tttgagtatc caatttttta acggacataa ctatcacaaa      240

ggtatcgatt ggtacatgga attcttccca attccgtcta atacgacatc tgacttttat      300

ttcgagcatt cggggaatta ctttgattcc gaggtagccc cacgccgtgc cgccgctctt      360

ttgcccaagg cgaaagtctt gactattctt attaatcccg cagaccgtgc ctaccgcgcg      420

tatgtatggc aacgcgcaca cgatgaccca gtcgcattga aatatacatt ccatgaggtg      480

attaccgcgg gtagtgacgc ttctagcaag ttacgtgctc ttcagaatcg ctgccttgtc      540

ccaggttggt atgccacaca catcgaacgt tggctgtccg cctaccatgc taatcagatt      600

cttgtgctgg atggtaaatt gttgcgtaca gagcctgcca aagttatgga tatggtgcaa      660

aaatttttgg gtgttacgaa tactattgat taccataaga cacttgcatt tgacccgaaa      720

aaaggtttct ggtgccaatt gttggagggt ggcaagacta agtgcttagg taagagtctt      780

ggttcgaagt accccgaaat ggatttagac tcgcgcgctt tcttgaagga ctattatcgt      840

gaccacaata tcgaactttc taaactttta tataagatgg gccaaacact tcccacgtgg      900

ctgcgtgaag acttgcagaa cacacgc                                          927


<210>  8
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 4

<400>  8

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His Gly Thr Gly Gly His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Phe Leu Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu His Ser Gly Asn Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Arg Ala Tyr Val Trp Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Leu 
                245                 250                 255     


Gly Lys Ser Leu Gly Ser Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  9
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 5

<400>  9
atgagcgaag agaaggaccc tttgtggcag gacccgtgcg aagataagcg tcataaagac       60

atttggagta aagagaagac ttgtgatcgt ttccccaagt tactgatcat cggcccacat      120

aagacaggag tacatgcatt gtacttgttt ttgggaatgc atccggacct gtcttcaaat      180

taccccagtt cagagacagg caatcacatc ggcttcttcg gaggacataa ctaccacaaa      240

ggcatcgatt ggtacatgga attctttcct atcccctcta atactacctc agatttttac      300

ttcgagaaaa gtgcttggta ctttgactcc gaagttgctc ctcgtcgcgc agcagcatta      360

cttccaaagg cgaaagttct gactattttg atcaaccctg cggatcgcgc ctacagctgg      420

tatcaacacc agcgcgccca cgatgatcct gtcgcattga aatacacctt tcatgaagtt      480

atcaccgctg gctccgatgc gtctagcaaa ttgcgtgcat tacagaatcg ttgccttgtg      540

ccaggatggt acgctaccca tattgagcgc tggctgagtg catatcacgc gaatcagatt      600

ctggtgttag atggaaagct gctgcgtact gaaccggcca aagtaatgga catggttcaa      660

aagttcctgg gggtgacgaa cacaattgat taccataaga ctcttgcatt tgatcctaag      720

aaaggctttt ggtgtcaact tttagagggg gggaagacca agtgcttagg gaagagcgtg      780

ggacgcaagt accccgaaat ggacttagat agccgtgctt tcttgaagga ttattatcgc      840

gaccacaaca ttgaactttc taaactgtta tacaagatgg gccagacact gccgacctgg      900

ctgcgtgaag acttgcagaa cacacgc                                          927


<210>  10
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 5

<400>  10

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His Lys Thr Gly Val His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Gly Asn His Ile Gly Phe Phe Gly Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Lys Ser Ala Trp Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Leu 
                245                 250                 255     


Gly Lys Ser Val Gly Arg Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  11
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 6

<400>  11
atgagcgaag agaaggaccc tttgtggcag gacccgtgcg aagataagcg tcacaaggat       60

atttggtcca aagaaaagac ctgcgatcgc tttcccaagc tgttaatcat cggcccggcc      120

aaaacaggcg cctggctttt gcatcatttc ctgggcatgc atcccgactt gtcgagtaac      180

tatccgtcgt ccgaaactca ctcctctatt caattcttca atgggcataa ttatcacaag      240

ggtatcgact ggtacatgga gttctttcca atccctagta atacaaccag tgatttttat      300

tttgagacta gcgctaacta ctttgattca gaggtggcac cgcgtcgtgc ggcggcgctg      360

ttgccgaagg cgaaagtttt aactatcttg atcaatccgg cagatcgtgc gtacagctgg      420

taccaacatc aacgtgctca cgatgacccg gtggccctga aatatacctt ccacgaggtc      480

attacagccg gaagtgacgc ttccagtaaa ttgcgcgcgt tacaaaatcg ttgtctggtc      540

cctgggtggt acgcaacgca cattgaacgc tggttatcgg cataccacgc aaatcagatc      600

cttgtgcttg acggaaagtt attgcgtact gaaccggcca aggtgatgga tatggtacag      660

aaattccttg gcgtcaccaa tacgatcgac tatcacaaga cgcttgcctt cgaccccaag      720

aaggggtttt ggtgccaact tttagagggt ggtaagacaa agtgtgctca taaggggtta      780

ggccgcaagt accctgaaat ggatctggac tcgcgcgctt ttttgaaaga ctattatcgc      840

gatcacaata ttgagttgag caagttgctg tataaaatgg gacagacact gccgacctgg      900

ctgcgtgaag acttgcagaa cacacgc                                          927


<210>  12
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 6

<400>  12

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Ala Lys Thr Gly Ala Trp Leu Leu His 
        35                  40                  45              


His Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr His Ser Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Thr Ser Ala Asn Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Ala 
                245                 250                 255     


His Lys Gly Leu Gly Arg Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  13
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 7


<220>
<221>  MISC_FEATURE
<222>  (40)..(40)
<223>  Xaa is glutamine, serine, or alanine

<220>
<221>  MISC_FEATURE
<222>  (45)..(45)
<223>  Xaa is tryptophan or phenyalanine

<220>
<221>  MISC_FEATURE
<222>  (46)..(46)
<223>  Xaa is alanine or leucine

<220>
<221>  MISC_FEATURE
<222>  (48)..(48)
<223>  Xaa is tyrosine, threonine, or histidine

<220>
<221>  MISC_FEATURE
<222>  (67)..(67)
<223>  Xaa is histidine or glycine

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is glycine, histidine, or serine

<220>
<221>  MISC_FEATURE
<222>  (103)..(103)
<223>  Xaa is lysine or threonine

<220>
<221>  MISC_FEATURE
<222>  (105)..(105)
<223>  Xaa is alanine or serine

<220>
<221>  MISC_FEATURE
<222>  (256)..(256)
<223>  Xaa is leucine, histidine, or alanine

<220>
<221>  MISC_FEATURE
<222>  (257)..(257)
<223>  Xaa is glycine or histidine

<220>
<221>  MISC_FEATURE
<222>  (259)..(259)
<223>  Xaa is serine, arginine, or glycine

<220>
<221>  MISC_FEATURE
<222>  (260)..(260)
<223>  Xaa is histidine, tryptophan, or leucine

<400>  13

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Xaa Lys Thr Gly Ala Xaa Xaa Leu Xaa 
        35                  40                  45              


His Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Xaa Xaa Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Xaa Ser Xaa Asn Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Xaa 
                245                 250                 255     


Xaa Lys Xaa Xaa Gly Arg Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  14
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 8


<220>
<221>  MISC_FEATURE
<222>  (41)..(41)
<223>  Xaa is glycine or lysine

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa is glycine or valine

<220>
<221>  MISC_FEATURE
<222>  (67)..(67)
<223>  Xaa is glycine or phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is glutamic acid, leucine, or asparagine

<220>
<221>  MISC_FEATURE
<222>  (69)..(69)
<223>  Xaa is glutamic acid, serine, or histidine

<220>
<221>  MISC_FEATURE
<222>  (71)..(71)
<223>  Xaa is glutamine or glycine

<220>
<221>  MISC_FEATURE
<222>  (74)..(74)
<223>  Xaa is asparagine or glycine

<220>
<221>  MISC_FEATURE
<222>  (103)..(103)
<223>  Xaa is lysine or histidine

<220>
<221>  MISC_FEATURE
<222>  (105)..(105)
<223>  Xaa is alanine or glycine

<220>
<221>  MISC_FEATURE
<222>  (106)..(106)
<223>  Xaa is asparagine or tryptophan

<220>
<221>  MISC_FEATURE
<222>  (139)..(139)
<223>  Xaa is serine or arginine

<220>
<221>  MISC_FEATURE
<222>  (140)..(140)
<223>  Xaa is tryptophan or alanine

<220>
<221>  MISC_FEATURE
<222>  (142)..(142)
<223>  Xaa is glutamine or valine

<220>
<221>  MISC_FEATURE
<222>  (143)..(143)
<223>  Xaa is histidine, alanine, or tryptophan

<220>
<221>  MISC_FEATURE
<222>  (256)..(256)
<223>  Xaa is leucine or glycine

<220>
<221>  MISC_FEATURE
<222>  (259)..(259)
<223>  Xaa is serine or histidine

<220>
<221>  MISC_FEATURE
<222>  (260)..(260)
<223>  Xaa is leucine or valine

<220>
<221>  MISC_FEATURE
<222>  (262)..(262)
<223>  Xaa is arginine or serine

<400>  14

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His Xaa Thr Gly Xaa His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Met His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Xaa Xaa Xaa Ile Xaa Phe Phe Xaa Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Glu Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Xaa Ser Xaa Xaa Tyr Phe Asp Ser Glu Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Xaa Xaa Tyr Xaa Xaa Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Val Ala Leu Lys Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Ser Asp Ala Ser Ser Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Ile Glu Arg Trp Leu 
            180                 185                 190         


Ser Ala Tyr His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Lys Val Met Asp Met Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Xaa 
                245                 250                 255     


Gly Lys Xaa Xaa Gly Xaa Lys Tyr Pro Glu Met Asp Leu Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Lys Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Asp 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  15
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 9

<400>  15

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Gln Lys Thr Gly Ala Trp Ala Leu Tyr 
        35                  40                  45              


His Phe Leu Gly Leu His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr His Gly Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Asp Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Lys Ser Ala Asn Tyr Phe Asp Ser Asp Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Ala Ala Leu Arg Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Pro Asp Ala Ser Leu Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Leu Glu Arg Trp Leu 
            180                 185                 190         


Gly Ala Phe His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Arg Val Met Asp Thr Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Leu 
                245                 250                 255     


Gly Arg Ser His Gly Arg Lys Tyr Pro Asp Met Asp Pro Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Arg Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Glu 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  16
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 10

<400>  16

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Ser Lys Thr Gly Ala Phe Leu Leu Thr 
        35                  40                  45              


His Phe Leu Gly Leu His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Gly His Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Asp Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Thr Ser Ser Asn Tyr Phe Asp Ser Asp Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Ala Ala Leu Arg Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Pro Asp Ala Ser Leu Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Leu Glu Arg Trp Leu 
            180                 185                 190         


Gly Ala Phe His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Arg Val Met Asp Thr Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys His 
                245                 250                 255     


Gly Arg Arg Trp Gly Arg Lys Tyr Pro Asp Met Asp Pro Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Arg Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Glu 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  17
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 11

<400>  17

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His His Thr Gly Gly His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Leu His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Gly Glu Glu Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Asp Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Lys Ser Ala Asn Tyr Phe Asp Ser Asp Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln Ala Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Ala Ala Leu Arg Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Pro Asp Ala Ser Leu Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Leu Glu Arg Trp Leu 
            180                 185                 190         


Gly Ala Phe His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Arg Val Met Asp Thr Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Gly 
                245                 250                 255     


Gly Arg His Leu Gly Arg Lys Tyr Pro Asp Met Asp Pro Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Arg Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Glu 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  18
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 12

<400>  18

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His Gly Thr Gly Gly His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Leu His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Phe Leu Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Asp Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu His Ser Gly Asn Tyr Phe Asp Ser Asp Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Arg Ala Tyr Val Trp Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Ala Ala Leu Arg Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Pro Asp Ala Ser Leu Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Leu Glu Arg Trp Leu 
            180                 185                 190         


Gly Ala Phe His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Arg Val Met Asp Thr Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Leu 
                245                 250                 255     


Gly Arg Ser Leu Gly Ser Lys Tyr Pro Asp Met Asp Pro Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Arg Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Glu 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  19
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 13

<400>  19

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro His Lys Thr Gly Val His Ala Leu Tyr 
        35                  40                  45              


Leu Phe Leu Gly Leu His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr Gly Asn His Ile Gly Phe Phe Gly Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Asp Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Lys Ser Ala Trp Tyr Phe Asp Ser Asp Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Ala Ala Leu Arg Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Pro Asp Ala Ser Leu Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Leu Glu Arg Trp Leu 
            180                 185                 190         


Gly Ala Phe His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Arg Val Met Asp Thr Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Leu 
                245                 250                 255     


Gly Arg Ser Val Gly Arg Lys Tyr Pro Asp Met Asp Pro Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Arg Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Glu 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  20
<211>  309
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent N-sulfotransferase variant 14

<400>  20

Met Ser Glu Glu Lys Asp Pro Leu Trp Gln Asp Pro Cys Glu Asp Lys 
1               5                   10                  15      


Arg His Lys Asp Ile Trp Ser Lys Glu Lys Thr Cys Asp Arg Phe Pro 
            20                  25                  30          


Lys Leu Leu Ile Ile Gly Pro Ala Lys Thr Gly Ala Trp Leu Leu His 
        35                  40                  45              


His Phe Leu Gly Leu His Pro Asp Leu Ser Ser Asn Tyr Pro Ser Ser 
    50                  55                  60                  


Glu Thr His Ser Ser Ile Gln Phe Phe Asn Gly His Asn Tyr His Lys 
65                  70                  75                  80  


Gly Ile Asp Trp Tyr Met Asp Phe Phe Pro Ile Pro Ser Asn Thr Thr 
                85                  90                  95      


Ser Asp Phe Tyr Phe Glu Thr Ser Ala Asn Tyr Phe Asp Ser Asp Val 
            100                 105                 110         


Ala Pro Arg Arg Ala Ala Ala Leu Leu Pro Lys Ala Lys Val Leu Thr 
        115                 120                 125             


Ile Leu Ile Asn Pro Ala Asp Arg Ala Tyr Ser Trp Tyr Gln His Gln 
    130                 135                 140                 


Arg Ala His Asp Asp Pro Ala Ala Leu Arg Tyr Thr Phe His Glu Val 
145                 150                 155                 160 


Ile Thr Ala Gly Pro Asp Ala Ser Leu Lys Leu Arg Ala Leu Gln Asn 
                165                 170                 175     


Arg Cys Leu Val Pro Gly Trp Tyr Ala Thr His Leu Glu Arg Trp Leu 
            180                 185                 190         


Gly Ala Phe His Ala Asn Gln Ile Leu Val Leu Asp Gly Lys Leu Leu 
        195                 200                 205             


Arg Thr Glu Pro Ala Arg Val Met Asp Thr Val Gln Lys Phe Leu Gly 
    210                 215                 220                 


Val Thr Asn Thr Ile Asp Tyr His Lys Thr Leu Ala Phe Asp Pro Lys 
225                 230                 235                 240 


Lys Gly Phe Trp Cys Gln Leu Leu Glu Gly Gly Lys Thr Lys Cys Ala 
                245                 250                 255     


His Arg Gly Leu Gly Arg Lys Tyr Pro Asp Met Asp Pro Asp Ser Arg 
            260                 265                 270         


Ala Phe Leu Arg Asp Tyr Tyr Arg Asp His Asn Ile Glu Leu Ser Lys 
        275                 280                 285             


Leu Leu Tyr Lys Met Gly Gln Thr Leu Pro Thr Trp Leu Arg Glu Glu 
    290                 295                 300                 


Leu Gln Asn Thr Arg 
305                 


<210>  21
<211>  939
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 1

<400>  21
atgaagtact attttccggt ccgcgaattg gagcgctcac tgcgttttga tatgaagggt       60

gacgatgtga ttgtattcct tcatattggg catacaggcg ggacgacttt tggacgtcat      120

ttagtccaga acgttcgtct ggaggtaccc tgtgattgcc gcccgggtca aaaaaaatgc      180

acgtgttacc gcccaaatcg ccgtgagacc tggttgttct ctcgcttttc cacaggctgg      240

tcttgcggaa ctaacgccga ctggacagag cttaccaact gtgtcccagg ggtattggac      300

cgccgtgatc cagctgggtt gcgctcgcca cgtaaatttt actatattac cctgctgcgc      360

gatcctgtct cccgctacct ggggggctgg cgccaccatc agcgtggcgg cacaaataaa      420

acatcgttgc acatgtgtga tgggcgcacg ccaacacccg aagagcttcc cccgtgctat      480

gagggaacgg actggagtgg atgtacttta caggaattta tggactgtcc ctacaatttg      540

gcaaacaatc gtcaagtccg catgcttgcg gatcttagtt tggtcggctg ttacaacttg      600

agctttattc ccgaaagtaa gcgcgcacaa cttttattag agagtgccaa gaagaacttg      660

cgtggaatgg cattctttgg attgaccgaa tttcagcgta aaacgcagta tttgtttgaa      720

cgtacattca acctgaaatt tatccgcccg tttatgcagt ataacagtac gcgcgcgggg      780

ggcgtggaag tggacgagga cacgattcgc cacattgagg aattgaatga ccttgatatg      840

caattgtacg actacgccaa agatcttttc cagcaacgtt atcaatacaa gcgccagctt      900

gaacgccgcg agcagcgttt acgcaatcgt gaggaataa                             939


<210>  22
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 1

<400>  22

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu Arg Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Thr Asn Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Gly Leu Arg Ser Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Gly 
        115                 120                 125             


Gly Trp Arg His His Gln Arg Gly Gly Thr Asn Lys Thr Ser Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Ala Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Ser Lys Arg 
        195                 200                 205             


Ala Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Asp Glu Asp Thr Ile Arg His Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Lys Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Glu 
    290                 295                 300                 


Gln Arg Leu Arg Asn Arg Glu Glu 
305                 310         


<210>  23
<211>  939
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 2

<400>  23
atgaagtact attttccggt ccgcgaattg gagcgctcat tgcgtttcga tatgaagggt       60

gatgatgtca tcgtcttcct tcacattggt cacactggtg gaaccacctt tggacgtcat      120

cttgtgcaaa acgtacgttt agaggtccct tgcgattgtc gtccgggtca aaaaaaatgt      180

acttgctatc gtcctaatcg tcgtgaaacg tggcttttca gtcgttttag tacggggtgg      240

tcatgcggta cccgcgcaga ctggacggag ttaaccaact gcgtacctgg ggtgttggat      300

cgccgcgatc cggcaggttt acgctcccca cgtaaattct attatattac cctgttacgt      360

gacccagtca gtcgctattt gtctcactgg cgtcacacac aacgtggcgg cgcgaacaag      420

accggactgc acatgtgtga cgggcgtact cctacaccag aggaattacc cccatgctat      480

gagggaactg actggtcggg atgtacactg caggagttca tggactgccc atacaatctg      540

gggaataatc gccaagtccg tatgttggcg gatttaagcc ttgtcggatg ctataatttg      600

tcattcattc cagaatcaaa acgcgcgcaa cttcttcttg agtcagccaa gaaaaatttg      660

cgcggaatgg catttttcgg gttgacagaa tttcagcgca aaacacaata tctgttcgag      720

cgcacattca atttaaaatt tattcgtcct ttcatgcaat acaactctac acgtgcagga      780

ggagtcgaag tggacgagga cacaattcgc cacatcgagg aattaaatga tctggatatg      840

cagttgtatg actatgcaaa agatctgttt cagcaacgct atcaatacaa gcgtcagttg      900

gaacgccgcg agcagcgttt acgcaatcgt gaggaataa                             939


<210>  24
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 2

<400>  24

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu Arg Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Thr Arg Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Gly Leu Arg Ser Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Ser 
        115                 120                 125             


His Trp Arg His Thr Gln Arg Gly Gly Ala Asn Lys Thr Gly Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Ser Lys Arg 
        195                 200                 205             


Ala Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Asp Glu Asp Thr Ile Arg His Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Lys Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Glu 
    290                 295                 300                 


Gln Arg Leu Arg Asn Arg Glu Glu 
305                 310         


<210>  25
<211>  939
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 3

<400>  25
atgaagtact attttccggt ccgcgaattg gagcgctcat tgcgtttcga catgaaagga       60

gacgacgtca ttgtattttt acatattggc cacaccggtg gcacgacttt tggccgtcac      120

ttagtccaaa acgtacgctt agaggtgcct tgcgactgtc gtccagggca aaagaaatgc      180

acctgctatc gccccaaccg ccgtgaaaca tggttgttta gtcgctttag taccggttgg      240

agctgtggct ctcatgctga ttggactgaa ctgacgaatt gtgtccccgg agtattggat      300

cgccgtgatc ctgctggttt acgctcacct cgcaaattct attatattac gttacttcgt      360

gatcccgtta gccgttatct tagtgggtgg cgtcaccatc aacgcggagg ggctaataag      420

acgagcctgc acatgtgtga cggacgtacg ccaacccccg aggaactgcc gccctgttac      480

gaggggacgg actggtctgg ctgtacatta caagagttta tggattgccc atataacctg      540

ggtaacaatc gccaagtccg tatgttggcg gatctttcgc tggtgggatg ttataattta      600

agttttatcc cggagagcaa gcgtgcacag ttgctgcttg aatcagcgaa gaagaacctt      660

cgcggaatgg catttttcgg tttaacggag tttcaacgta agactcagta ccttttcgag      720

cgtaccttca acttgaaatt tatccgtccc ttcatgcagt acaactccac ccgcgctggt      780

ggagttgaag tcgacgagga taccatccgc cacattgaag aacttaatga cttagatatg      840

caattgtacg actatgctaa ggacttattc cagcaacgtt atcagtacaa acgccaattg      900

gaacgccgcg agcagcgttt acgcaatcgt gaggaataa                             939


<210>  26
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 3

<400>  26

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu Arg Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Ser His Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Gly Leu Arg Ser Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Ser 
        115                 120                 125             


Gly Trp Arg His His Gln Arg Gly Gly Ala Asn Lys Thr Ser Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Ser Lys Arg 
        195                 200                 205             


Ala Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Asp Glu Asp Thr Ile Arg His Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Lys Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Glu 
    290                 295                 300                 


Gln Arg Leu Arg Asn Arg Glu Glu 
305                 310         


<210>  27
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 4


<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (85)..(85)
<223>  Xaa is asparagine, arginine, or histidine

<220>
<221>  MISC_FEATURE
<222>  (128)..(128)
<223>  Xaa is glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (129)..(129)
<223>  Xaa is glycine or histidine

<220>
<221>  MISC_FEATURE
<222>  (133)..(133)
<223>  Xaa is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (138)..(138)
<223>  Xaa is alanine or threonine

<220>
<221>  MISC_FEATURE
<222>  (142)..(142)
<223>  Xaa is glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (181)..(181)
<223>  Xaa is glycine or alanine

<400>  27

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu Arg Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Xaa Xaa Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Gly Leu Arg Ser Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Xaa 
        115                 120                 125             


Xaa Trp Arg His Xaa Gln Arg Gly Gly Xaa Asn Lys Thr Xaa Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Xaa Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Ser Lys Arg 
        195                 200                 205             


Ala Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Asp Glu Asp Thr Ile Arg His Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Lys Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Glu 
    290                 295                 300                 


Gln Arg Leu Arg Asn Arg Glu Glu 
305                 310         


<210>  28
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 5


<220>
<221>  MISC_FEATURE
<222>  (149)..(149)
<223>  Xaa is threonine or serine

<220>
<221>  MISC_FEATURE
<222>  (150)..(150)
<223>  Xaa is asparagine, arginine, or histidine

<220>
<221>  MISC_FEATURE
<222>  (193)..(193)
<223>  Xaa is glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (194)..(194)
<223>  Xaa is glycine or histidine

<220>
<221>  MISC_FEATURE
<222>  (198)..(198)
<223>  Xaa is histidine or threonine

<220>
<221>  MISC_FEATURE
<222>  (203)..(203)
<223>  Xaa is alanine or threonine

<220>
<221>  MISC_FEATURE
<222>  (207)..(207)
<223>  Xaa is glycine or serine

<220>
<221>  MISC_FEATURE
<222>  (246)..(246)
<223>  Xaa is glycine or alanine

<400>  28

Met Arg Arg Arg Arg Ala Gly Gly Arg Thr Met Val Glu Arg Ala Ser 
1               5                   10                  15      


Lys Phe Val Leu Val Val Ala Gly Ser Ala Cys Phe Met Leu Ile Leu 
            20                  25                  30          


Tyr Gln Tyr Ala Gly Pro Gly Leu Ser Leu Gly Ala Pro Gly Gly Arg 
        35                  40                  45              


Val Pro Pro Asp Asp Leu Asp Leu Phe Pro Thr Pro Asp Pro His Tyr 
    50                  55                  60                  


Glu Lys Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu Arg 
65                  70                  75                  80  


Phe Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His 
                85                  90                  95      


Thr Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu 
            100                 105                 110         


Glu Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr 
        115                 120                 125             


Arg Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly 
    130                 135                 140                 


Trp Ser Cys Gly Xaa Xaa Ala Asp Trp Thr Glu Leu Thr Asn Cys Val 
145                 150                 155                 160 


Pro Gly Val Leu Asp Arg Arg Asp Pro Ala Gly Leu Arg Ser Pro Arg 
                165                 170                 175     


Lys Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu 
            180                 185                 190         


Xaa Xaa Trp Arg His Xaa Gln Arg Gly Gly Xaa Asn Lys Thr Xaa Leu 
        195                 200                 205             


His Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys 
    210                 215                 220                 


Tyr Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp 
225                 230                 235                 240 


Cys Pro Tyr Asn Leu Xaa Asn Asn Arg Gln Val Arg Met Leu Ala Asp 
                245                 250                 255     


Leu Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Ser Lys 
            260                 265                 270         


Arg Ala Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met 
        275                 280                 285             


Ala Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe 
    290                 295                 300                 


Glu Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Arg Ala Gly Gly Val Glu Val Asp Glu Asp Thr Ile Arg His 
                325                 330                 335     


Ile Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Lys 
            340                 345                 350         


Asp Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg 
        355                 360                 365             


Glu Gln Arg Leu Arg Asn Arg Glu Glu Arg Leu Leu His Arg Ser Lys 
    370                 375                 380                 


Glu Ala Leu Pro Arg Glu Asp Pro Glu Glu Pro Gly Arg Val Pro Thr 
385                 390                 395                 400 


Glu Asp Tyr Met Ser His Ile Ile Glu Lys Trp 
                405                 410     


<210>  29
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 6

<400>  29

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu His Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Thr Asn Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Ala Leu Arg Thr Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Gly 
        115                 120                 125             


Gly Trp Arg His His Gln Arg Gly Gly Thr Asn Lys Thr Ser Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Ala Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Gly Lys Arg 
        195                 200                 205             


Ser Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Gly Glu Asp Thr Ile Arg Arg Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Arg Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Gln 
    290                 295                 300                 


Gln Arg Leu Arg Ser Arg Glu Glu 
305                 310         


<210>  30
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 7

<400>  30

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu His Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Thr Arg Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Ala Leu Arg Thr Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Ser 
        115                 120                 125             


His Trp Arg His Thr Gln Arg Gly Gly Ala Asn Lys Thr Gly Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Gly Lys Arg 
        195                 200                 205             


Ser Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Gly Glu Asp Thr Ile Arg Arg Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Arg Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Gln 
    290                 295                 300                 


Gln Arg Leu Arg Ser Arg Glu Glu 
305                 310         


<210>  31
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 8

<400>  31

Met Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu His Phe 
1               5                   10                  15      


Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His Thr 
            20                  25                  30          


Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu Glu 
        35                  40                  45              


Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr Arg 
    50                  55                  60                  


Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp 
65                  70                  75                  80  


Ser Cys Gly Ser His Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro 
                85                  90                  95      


Gly Val Leu Asp Arg Arg Asp Pro Ala Ala Leu Arg Thr Pro Arg Lys 
            100                 105                 110         


Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu Ser 
        115                 120                 125             


Gly Trp Arg His His Gln Arg Gly Gly Ala Asn Lys Thr Ser Leu His 
    130                 135                 140                 


Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys Tyr 
145                 150                 155                 160 


Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp Cys 
                165                 170                 175     


Pro Tyr Asn Leu Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu 
            180                 185                 190         


Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Gly Lys Arg 
        195                 200                 205             


Ser Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met Ala 
    210                 215                 220                 


Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe Glu 
225                 230                 235                 240 


Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn Ser 
                245                 250                 255     


Thr Arg Ala Gly Gly Val Glu Val Gly Glu Asp Thr Ile Arg Arg Ile 
            260                 265                 270         


Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Arg Asp 
        275                 280                 285             


Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg Gln 
    290                 295                 300                 


Gln Arg Leu Arg Ser Arg Glu Glu 
305                 310         


<210>  32
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 9

<400>  32

Met Arg Arg Arg Arg Ala Gly Ser Arg Thr Met Val Glu Arg Ala Ser 
1               5                   10                  15      


Lys Phe Val Leu Val Val Ala Gly Ser Ala Cys Phe Met Leu Ile Leu 
            20                  25                  30          


Tyr Gln Tyr Ala Gly Pro Gly Leu Ser Leu Gly Ala Pro Gly Gly Arg 
        35                  40                  45              


Ala Pro Pro Asp Asp Leu Asp Leu Phe Pro Thr Pro Asp Pro His Tyr 
    50                  55                  60                  


Glu Lys Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu His 
65                  70                  75                  80  


Phe Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His 
                85                  90                  95      


Thr Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu 
            100                 105                 110         


Glu Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr 
        115                 120                 125             


Arg Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly 
    130                 135                 140                 


Trp Ser Cys Gly Thr Asn Ala Asp Trp Thr Glu Leu Thr Asn Cys Val 
145                 150                 155                 160 


Pro Gly Val Leu Asp Arg Arg Asp Pro Ala Ala Leu Arg Thr Pro Arg 
                165                 170                 175     


Lys Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu 
            180                 185                 190         


Gly Gly Trp Arg His His Gln Arg Gly Gly Thr Asn Lys Thr Ser Leu 
        195                 200                 205             


His Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys 
    210                 215                 220                 


Tyr Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp 
225                 230                 235                 240 


Cys Pro Tyr Asn Leu Ala Asn Asn Arg Gln Val Arg Met Leu Ala Asp 
                245                 250                 255     


Leu Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Gly Lys 
            260                 265                 270         


Arg Ser Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met 
        275                 280                 285             


Ala Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe 
    290                 295                 300                 


Glu Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Arg Ala Gly Gly Val Glu Val Gly Glu Asp Thr Ile Arg Arg 
                325                 330                 335     


Ile Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Arg 
            340                 345                 350         


Asp Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg 
        355                 360                 365             


Gln Gln Arg Leu Arg Ser Arg Glu Glu Arg Leu Leu His Arg Ala Lys 
    370                 375                 380                 


Glu Ala Pro Pro Arg Gly Asp Thr Glu Glu Pro Gly Arg Val Pro Thr 
385                 390                 395                 400 


Glu Asp Tyr Met Ser His Ile Ile Glu Lys Trp 
                405                 410     


<210>  33
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 10

<400>  33

Met Arg Arg Arg Arg Ala Gly Ser Arg Thr Met Val Glu Arg Ala Ser 
1               5                   10                  15      


Lys Phe Val Leu Val Val Ala Gly Ser Ala Cys Phe Met Leu Ile Leu 
            20                  25                  30          


Tyr Gln Tyr Ala Gly Pro Gly Leu Ser Leu Gly Ala Pro Gly Gly Arg 
        35                  40                  45              


Ala Pro Pro Asp Asp Leu Asp Leu Phe Pro Thr Pro Asp Pro His Tyr 
    50                  55                  60                  


Glu Lys Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu His 
65                  70                  75                  80  


Phe Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His 
                85                  90                  95      


Thr Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu 
            100                 105                 110         


Glu Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr 
        115                 120                 125             


Arg Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly 
    130                 135                 140                 


Trp Ser Cys Gly Thr Arg Ala Asp Trp Thr Glu Leu Thr Asn Cys Val 
145                 150                 155                 160 


Pro Gly Val Leu Asp Arg Arg Asp Pro Ala Ala Leu Arg Thr Pro Arg 
                165                 170                 175     


Lys Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu 
            180                 185                 190         


Ser His Trp Arg His Thr Gln Arg Gly Gly Ala Asn Lys Thr Gly Leu 
        195                 200                 205             


His Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys 
    210                 215                 220                 


Tyr Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp 
225                 230                 235                 240 


Cys Pro Tyr Asn Leu Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp 
                245                 250                 255     


Leu Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Gly Lys 
            260                 265                 270         


Arg Ser Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met 
        275                 280                 285             


Ala Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe 
    290                 295                 300                 


Glu Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Arg Ala Gly Gly Val Glu Val Gly Glu Asp Thr Ile Arg Arg 
                325                 330                 335     


Ile Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Arg 
            340                 345                 350         


Asp Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg 
        355                 360                 365             


Gln Gln Arg Leu Arg Ser Arg Glu Glu Arg Leu Leu His Arg Ala Lys 
    370                 375                 380                 


Glu Ala Pro Pro Arg Gly Asp Thr Glu Glu Pro Gly Arg Val Pro Thr 
385                 390                 395                 400 


Glu Asp Tyr Met Ser His Ile Ile Glu Lys Trp 
                405                 410     


<210>  34
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 11

<400>  34

Met Arg Arg Arg Arg Ala Gly Ser Arg Thr Met Val Glu Arg Ala Ser 
1               5                   10                  15      


Lys Phe Val Leu Val Val Ala Gly Ser Ala Cys Phe Met Leu Ile Leu 
            20                  25                  30          


Tyr Gln Tyr Ala Gly Pro Gly Leu Ser Leu Gly Ala Pro Gly Gly Arg 
        35                  40                  45              


Ala Pro Pro Asp Asp Leu Asp Leu Phe Pro Thr Pro Asp Pro His Tyr 
    50                  55                  60                  


Glu Lys Lys Tyr Tyr Phe Pro Val Arg Glu Leu Glu Arg Ser Leu His 
65                  70                  75                  80  


Phe Asp Met Lys Gly Asp Asp Val Ile Val Phe Leu His Ile Gly His 
                85                  90                  95      


Thr Gly Gly Thr Thr Phe Gly Arg His Leu Val Gln Asn Val Arg Leu 
            100                 105                 110         


Glu Val Pro Cys Asp Cys Arg Pro Gly Gln Lys Lys Cys Thr Cys Tyr 
        115                 120                 125             


Arg Pro Asn Arg Arg Glu Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly 
    130                 135                 140                 


Trp Ser Cys Gly Ser His Ala Asp Trp Thr Glu Leu Thr Asn Cys Val 
145                 150                 155                 160 


Pro Gly Val Leu Asp Arg Arg Asp Pro Ala Ala Leu Arg Thr Pro Arg 
                165                 170                 175     


Lys Phe Tyr Tyr Ile Thr Leu Leu Arg Asp Pro Val Ser Arg Tyr Leu 
            180                 185                 190         


Ser Gly Trp Arg His His Gln Arg Gly Gly Ala Asn Lys Thr Ser Leu 
        195                 200                 205             


His Met Cys Asp Gly Arg Thr Pro Thr Pro Glu Glu Leu Pro Pro Cys 
    210                 215                 220                 


Tyr Glu Gly Thr Asp Trp Ser Gly Cys Thr Leu Gln Glu Phe Met Asp 
225                 230                 235                 240 


Cys Pro Tyr Asn Leu Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp 
                245                 250                 255     


Leu Ser Leu Val Gly Cys Tyr Asn Leu Ser Phe Ile Pro Glu Gly Lys 
            260                 265                 270         


Arg Ser Gln Leu Leu Leu Glu Ser Ala Lys Lys Asn Leu Arg Gly Met 
        275                 280                 285             


Ala Phe Phe Gly Leu Thr Glu Phe Gln Arg Lys Thr Gln Tyr Leu Phe 
    290                 295                 300                 


Glu Arg Thr Phe Asn Leu Lys Phe Ile Arg Pro Phe Met Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Arg Ala Gly Gly Val Glu Val Gly Glu Asp Thr Ile Arg Arg 
                325                 330                 335     


Ile Glu Glu Leu Asn Asp Leu Asp Met Gln Leu Tyr Asp Tyr Ala Arg 
            340                 345                 350         


Asp Leu Phe Gln Gln Arg Tyr Gln Tyr Lys Arg Gln Leu Glu Arg Arg 
        355                 360                 365             


Gln Gln Arg Leu Arg Ser Arg Glu Glu Arg Leu Leu His Arg Ala Lys 
    370                 375                 380                 


Glu Ala Pro Pro Arg Gly Asp Thr Glu Glu Pro Gly Arg Val Pro Thr 
385                 390                 395                 400 


Glu Asp Tyr Met Ser His Ile Ile Glu Lys Trp 
                405                 410     


<210>  35
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 12

<400>  35

Met Asp Glu Arg Phe Asn Lys Trp Leu Leu Thr Pro Val Leu Thr Leu 
1               5                   10                  15      


Leu Phe Val Val Ile Met Tyr Gln Tyr Val Ser Pro Ser Cys Thr Ser 
            20                  25                  30          


Ser Cys Thr Asn Phe Gly Glu Gln Leu Arg Ser Gly Glu Ala Arg Pro 
        35                  40                  45              


Pro Ala Val Pro Ser Pro Ala Arg Arg Ala Gln Ala Pro Leu Asp Glu 
    50                  55                  60                  


Trp Glu Arg Arg Pro Gln Leu Pro Pro Pro Pro Arg Gly Pro Pro Glu 
65                  70                  75                  80  


Gly Ser Arg Gly Val Ala Ala Pro Glu Asp Glu Asp Glu Asp Pro Gly 
                85                  90                  95      


Asp Pro Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Pro Asp Pro Glu 
            100                 105                 110         


Ala Pro Glu Asn Gly Ser Leu Pro Arg Phe Val Pro Arg Phe Asn Phe 
        115                 120                 125             


Thr Leu Lys Asp Leu Thr Arg Phe Val Asp Phe Asn Ile Lys Gly Arg 
    130                 135                 140                 


Asp Val Ile Val Phe Leu His Ile Gly His Thr Gly Gly Thr Thr Phe 
145                 150                 155                 160 


Gly Arg His Leu Val Lys Asn Ile Arg Leu Glu Gln Pro Cys Ser Cys 
                165                 170                 175     


Lys Ala Gly Gln Lys Lys Cys Thr Cys His Arg Pro Gly Lys Lys Glu 
            180                 185                 190         


Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp Ser Cys Gly Thr Asn 
        195                 200                 205             


Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro Ala Ile Met Glu Lys 
    210                 215                 220                 


Lys Asp Cys Pro Arg Asn His Ser His Thr Arg Asn Phe Tyr Tyr Ile 
225                 230                 235                 240 


Thr Met Leu Arg Asp Pro Val Ser Arg Tyr Leu Gly Gly Trp Lys His 
                245                 250                 255     


His Gln Arg Gly Gly Thr Asn Lys Thr Ser Leu His Met Cys Asp Gly 
            260                 265                 270         


Arg Ser Pro Thr Pro Asp Glu Leu Pro Thr Cys Tyr Pro Gly Asp Asp 
        275                 280                 285             


Trp Ser Gly Val Ser Leu Arg Glu Phe Met Asp Cys Ser Tyr Asn Leu 
    290                 295                 300                 


Ala Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu Ser Leu Val Gly 
305                 310                 315                 320 


Cys Tyr Asn Leu Thr Phe Met Asn Glu Ser Glu Arg Asn Thr Ile Leu 
                325                 330                 335     


Leu Gln Ser Ala Lys Asn Asn Leu Lys Asn Met Ala Phe Phe Gly Leu 
            340                 345                 350         


Thr Glu Phe Gln Arg Lys Thr Gln Phe Leu Phe Glu Arg Thr Phe Asn 
        355                 360                 365             


Leu Lys Phe Ile Ser Pro Phe Thr Gln Val Asn Ile Thr Arg Ala Ser 
    370                 375                 380                 


Asn Val Asp Ile Asn Asp Gly Ala Arg Gln His Ile Glu Glu Leu Asn 
385                 390                 395                 400 


Phe Leu Asp Met Gln Leu Tyr Glu Tyr Ala Lys Asp Leu Phe Gln Gln 
                405                 410                 415     


Arg Tyr His His Thr Lys Gln Leu Glu His Gln Arg Asp Arg Gln Lys 
            420                 425                 430         


Arg Arg Glu Glu Arg Arg Leu Gln Arg Glu His Arg Ala His Arg Trp 
        435                 440                 445             


Pro Lys Glu Asp Arg Ala Met Glu Gly Thr Val Thr Glu Asp Tyr Asn 
    450                 455                 460                 


Ser Gln Val Val Arg Trp 
465                 470 


<210>  36
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 13

<400>  36

Met Asp Glu Arg Phe Asn Lys Trp Leu Leu Thr Pro Val Leu Thr Leu 
1               5                   10                  15      


Leu Phe Val Val Ile Met Tyr Gln Tyr Val Ser Pro Ser Cys Thr Ser 
            20                  25                  30          


Ser Cys Thr Asn Phe Gly Glu Gln Leu Arg Ser Gly Glu Ala Arg Pro 
        35                  40                  45              


Pro Ala Val Pro Ser Pro Ala Arg Arg Ala Gln Ala Pro Leu Asp Glu 
    50                  55                  60                  


Trp Glu Arg Arg Pro Gln Leu Pro Pro Pro Pro Arg Gly Pro Pro Glu 
65                  70                  75                  80  


Gly Ser Arg Gly Val Ala Ala Pro Glu Asp Glu Asp Glu Asp Pro Gly 
                85                  90                  95      


Asp Pro Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Pro Asp Pro Glu 
            100                 105                 110         


Ala Pro Glu Asn Gly Ser Leu Pro Arg Phe Val Pro Arg Phe Asn Phe 
        115                 120                 125             


Thr Leu Lys Asp Leu Thr Arg Phe Val Asp Phe Asn Ile Lys Gly Arg 
    130                 135                 140                 


Asp Val Ile Val Phe Leu His Ile Gly His Thr Gly Gly Thr Thr Phe 
145                 150                 155                 160 


Gly Arg His Leu Val Lys Asn Ile Arg Leu Glu Gln Pro Cys Ser Cys 
                165                 170                 175     


Lys Ala Gly Gln Lys Lys Cys Thr Cys His Arg Pro Gly Lys Lys Glu 
            180                 185                 190         


Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp Ser Cys Gly Thr Arg 
        195                 200                 205             


Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro Ala Ile Met Glu Lys 
    210                 215                 220                 


Lys Asp Cys Pro Arg Asn His Ser His Thr Arg Asn Phe Tyr Tyr Ile 
225                 230                 235                 240 


Thr Met Leu Arg Asp Pro Val Ser Arg Tyr Leu Ser His Trp Lys His 
                245                 250                 255     


Thr Gln Arg Gly Gly Ala Asn Lys Thr Gly Leu His Met Cys Asp Gly 
            260                 265                 270         


Arg Ser Pro Thr Pro Asp Glu Leu Pro Thr Cys Tyr Pro Gly Asp Asp 
        275                 280                 285             


Trp Ser Gly Val Ser Leu Arg Glu Phe Met Asp Cys Ser Tyr Asn Leu 
    290                 295                 300                 


Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu Ser Leu Val Gly 
305                 310                 315                 320 


Cys Tyr Asn Leu Thr Phe Met Asn Glu Ser Glu Arg Asn Thr Ile Leu 
                325                 330                 335     


Leu Gln Ser Ala Lys Asn Asn Leu Lys Asn Met Ala Phe Phe Gly Leu 
            340                 345                 350         


Thr Glu Phe Gln Arg Lys Thr Gln Phe Leu Phe Glu Arg Thr Phe Asn 
        355                 360                 365             


Leu Lys Phe Ile Ser Pro Phe Thr Gln Phe Asn Ile Thr Arg Ala Ser 
    370                 375                 380                 


Asn Val Asp Ile Asn Asp Gly Ala Arg Gln His Ile Glu Glu Leu Asn 
385                 390                 395                 400 


Phe Leu Asp Met Gln Leu Tyr Glu Tyr Ala Lys Asp Leu Phe Gln Gln 
                405                 410                 415     


Arg Tyr His His Thr Lys Gln Leu Glu His Gln Arg Asp Arg Gln Lys 
            420                 425                 430         


Arg Arg Glu Glu Arg Arg Leu Gln Arg Glu His Arg Ala His Arg Trp 
        435                 440                 445             


Pro Lys Glu Asp Arg Ala Met Glu Gly Thr Val Thr Glu Asp Tyr Asn 
    450                 455                 460                 


Ser Gln Val Val Arg Trp 
465                 470 


<210>  37
<211>  470
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence encoding for engineered aryl 
       sulfate-dependent 6-O-sulfotransferase variant 13

<400>  37

Met Asp Glu Arg Phe Asn Lys Trp Leu Leu Thr Pro Val Leu Thr Leu 
1               5                   10                  15      


Leu Phe Val Val Ile Met Tyr Gln Tyr Val Ser Pro Ser Cys Thr Ser 
            20                  25                  30          


Ser Cys Thr Asn Phe Gly Glu Gln Leu Arg Ser Gly Glu Ala Arg Pro 
        35                  40                  45              


Pro Ala Val Pro Ser Pro Ala Arg Arg Ala Gln Ala Pro Leu Asp Glu 
    50                  55                  60                  


Trp Glu Arg Arg Pro Gln Leu Pro Pro Pro Pro Arg Gly Pro Pro Glu 
65                  70                  75                  80  


Gly Ser Arg Gly Val Ala Ala Pro Glu Asp Glu Asp Glu Asp Pro Gly 
                85                  90                  95      


Asp Pro Glu Glu Glu Glu Glu Glu Glu Glu Glu Glu Pro Asp Pro Glu 
            100                 105                 110         


Ala Pro Glu Asn Gly Ser Leu Pro Arg Phe Val Pro Arg Phe Asn Phe 
        115                 120                 125             


Thr Leu Lys Asp Leu Thr Arg Phe Val Asp Phe Asn Ile Lys Gly Arg 
    130                 135                 140                 


Asp Val Ile Val Phe Leu His Ile Gly His Thr Gly Gly Thr Thr Phe 
145                 150                 155                 160 


Gly Arg His Leu Val Lys Asn Ile Arg Leu Glu Gln Pro Cys Ser Cys 
                165                 170                 175     


Lys Ala Gly Gln Lys Lys Cys Thr Cys His Arg Pro Gly Lys Lys Glu 
            180                 185                 190         


Thr Trp Leu Phe Ser Arg Phe Ser Thr Gly Trp Ser Cys Gly Ser His 
        195                 200                 205             


Ala Asp Trp Thr Glu Leu Thr Asn Cys Val Pro Ala Ile Met Glu Lys 
    210                 215                 220                 


Lys Asp Cys Pro Arg Asn His Ser His Thr Arg Asn Phe Tyr Tyr Ile 
225                 230                 235                 240 


Thr Met Leu Arg Asp Pro Val Ser Arg Tyr Leu Ser Gly Trp Lys His 
                245                 250                 255     


His Gln Arg Gly Gly Ala Asn Lys Thr Ser Leu His Met Cys Asp Gly 
            260                 265                 270         


Arg Ser Pro Thr Pro Asp Glu Leu Pro Thr Cys Tyr Pro Gly Asp Asp 
        275                 280                 285             


Trp Ser Gly Val Ser Leu Arg Glu Phe Met Asp Cys Ser Tyr Asn Leu 
    290                 295                 300                 


Gly Asn Asn Arg Gln Val Arg Met Leu Ala Asp Leu Ser Leu Val Gly 
305                 310                 315                 320 


Cys Tyr Asn Leu Thr Phe Met Asn Glu Ser Glu Arg Asn Thr Ile Leu 
                325                 330                 335     


Leu Gln Ser Ala Lys Asn Asn Leu Lys Asn Met Ala Phe Phe Gly Leu 
            340                 345                 350         


Thr Glu Phe Gln Arg Lys Thr Gln Phe Leu Phe Glu Arg Thr Phe Asn 
        355                 360                 365             


Leu Lys Phe Ile Ser Pro Phe Thr Gln Phe Asn Ile Thr Arg Ala Ser 
    370                 375                 380                 


Asn Val Asp Ile Asn Asp Gly Ala Arg Gln His Ile Glu Glu Leu Asn 
385                 390                 395                 400 


Phe Leu Asp Met Gln Leu Tyr Glu Tyr Ala Lys Asp Leu Phe Gln Gln 
                405                 410                 415     


Arg Tyr His His Thr Lys Gln Leu Glu His Gln Arg Asp Arg Gln Lys 
            420                 425                 430         


Arg Arg Glu Glu Arg Arg Leu Gln Arg Glu His Arg Ala His Arg Trp 
        435                 440                 445             


Pro Lys Glu Asp Arg Ala Met Glu Gly Thr Val Thr Glu Asp Tyr Asn 
    450                 455                 460                 


Ser Gln Val Val Arg Trp 
465                 470 


<210>  38
<211>  394
<212>  PRT
<213>  Bacteroides eggerthii

<400>  38

Met Lys Lys Asn Ile Phe Ile Ile Cys Met Ala Met Ala Ala Gly Cys 
1               5                   10                  15      


Ile Thr Thr Leu Thr Ala Gln Val Lys Asn Ala Glu Thr Leu Val Pro 
            20                  25                  30          


Leu Thr Lys Arg Val Asn Val Gln Ala Asp Thr Ala Arg Leu Asp Gln 
        35                  40                  45              


Ile Ile Asp Gly Cys Trp Val Ala Val Gly Thr Asn Lys Lys His Ala 
    50                  55                  60                  


Ile Gln Arg Asp Phe Thr Arg Leu Phe Ala Gly Lys Pro Ser Tyr Arg 
65                  70                  75                  80  


Phe Glu Leu Arg Lys Glu Asp Asn Thr Leu Glu Gly Tyr Gly Lys Gly 
                85                  90                  95      


Glu Thr Lys Gly Arg Ala Glu Phe Ser Tyr Cys Tyr Ala Thr Ser Ala 
            100                 105                 110         


Asp Phe Lys Gly Leu Pro Ala Asp Ala Tyr Arg Lys Ala Gln Ile Thr 
        115                 120                 125             


Lys Thr Val Tyr His His Gly Lys Gly Ile Cys Pro Gln Gly Val Ser 
    130                 135                 140                 


Arg Asp Tyr Glu Phe Ser Val Tyr Ile Pro Ser Ala Leu Asp Ser Asn 
145                 150                 155                 160 


Val Ser Thr Ile Phe Ala Gln Trp His Gly Met Pro Asp Arg Thr Leu 
                165                 170                 175     


Val Gln Thr Pro Glu Gly Glu Val Lys Lys Leu Thr Val Asp Glu Phe 
            180                 185                 190         


Leu Glu Leu Asp Lys Thr Thr Ile Phe Lys Lys Asn Thr Gly His Glu 
        195                 200                 205             


Lys Val Ala Lys Leu Asp Lys Gln Gly Asn Pro Val Lys Asp Lys Lys 
    210                 215                 220                 


Gly Asn Pro Val Tyr Lys Ala Gly Lys Lys Asn Gly Trp Leu Val Glu 
225                 230                 235                 240 


Gln Gly Gly Tyr Pro Pro Leu Ala Phe Gly Phe Ser Gly Gly Trp Phe 
                245                 250                 255     


Tyr Ile Lys Ala Asn Ser Asp Arg Arg Trp Leu Thr Asp Lys Thr Asp 
            260                 265                 270         


Arg Cys Asn Ala Ser Pro Glu Lys Thr Pro Val Met Lys Pro Val Thr 
        275                 280                 285             


Ser Lys Tyr Lys Ser Ser Thr Ile Ala Tyr Lys Met Pro Phe Ala Asp 
    290                 295                 300                 


Phe Pro Lys Asp Cys Trp Val Thr Phe Arg Val His Ile Asp Trp Thr 
305                 310                 315                 320 


Thr Tyr Gly Lys Glu Ala Glu Asn Ile Val Lys Pro Gly Lys Leu Asp 
                325                 330                 335     


Val Gln Met Glu Tyr Thr Asp Lys Lys Lys Thr Val Lys Glu His Ile 
            340                 345                 350         


Val Asn Asn Glu Val Ile Gln Ile Gly Arg Asn Asp Asp Asp Gly Tyr 
        355                 360                 365             


Tyr Phe Lys Phe Gly Ile Tyr Arg Val Gly Asn Ser Thr Val Pro Val 
    370                 375                 380                 


Cys Tyr Asn Leu Ala Gly Tyr Lys Glu Glu 
385                 390                 


<210>  39
<211>  773
<212>  PRT
<213>  Bacteroides eggerthii

<400>  39

Met Lys Lys Ser Ile Leu Phe Ile Thr Ser Leu Phe Leu Cys Ile Phe 
1               5                   10                  15      


Cys Leu Lys Ser Asn Ala Gln Gln Ser Arg Thr Glu Val Thr Trp Glu 
            20                  25                  30          


Lys Met Glu Asp Val Thr Val Pro Ile Pro Pro Gln Val His Pro Arg 
        35                  40                  45              


Leu Tyr Val Arg Ser Ala Asp Leu Pro Asp Leu Lys Lys Arg Met Asn 
    50                  55                  60                  


His Pro His Val Lys Glu Val Leu Ala Thr Leu Asn Lys Leu Gly Lys 
65                  70                  75                  80  


Asp Arg Thr Pro Glu Glu Glu Ala Lys Val Lys Asp Arg Gly Phe Arg 
                85                  90                  95      


Tyr Tyr Phe Glu Met Arg Gly Val Thr Ser Arg Val Gln Val Gln Ala 
            100                 105                 110         


Leu Glu Tyr Leu Val Tyr Gly Asp Lys Lys Gln Ala Arg Arg Ala Ile 
        115                 120                 125             


Thr Ala Met Leu Asp Thr Leu Gln Asn Val Asn Tyr Gly Thr Gln Gly 
    130                 135                 140                 


Asp Leu Ser Arg Ala Ser Gly Val Met Leu Thr Cys Gly Ala Met Val 
145                 150                 155                 160 


Tyr Asp Trp Cys Tyr Asp Gln Met Lys Glu Ser Glu Lys Lys Ala Tyr 
                165                 170                 175     


Val Glu Ser Phe Ile Arg Ile Ala Lys Thr Met Glu Cys Gly Tyr Pro 
            180                 185                 190         


Pro Arg Asn Asn Glu Pro Ile Ala Gly His Ser Ser Glu Trp Met Ile 
        195                 200                 205             


Leu Arg Asp Met Leu Ser Ala Gly Ile Ala Ile Tyr Asp Glu Tyr Pro 
    210                 215                 220                 


Asp Met Tyr Asn Tyr Val Ile Lys Met Met Phe Lys Asp Tyr Leu Pro 
225                 230                 235                 240 


Val Arg Asn Tyr Ile Tyr Ser Gly His Asn Tyr His Gln Gly Thr Ser 
                245                 250                 255     


Tyr Val Asn Val Arg Phe Ser Asn Asp Leu Phe Ser Leu Trp Ile Leu 
            260                 265                 270         


Gln Arg Met Gly Ala Gly Ala Ile Tyr Asn Pro Ala Gln Gln Phe Val 
        275                 280                 285             


Leu Tyr Asp Phe Leu Tyr Arg Arg Arg Pro Asp Gly Gln Val Met Pro 
    290                 295                 300                 


Ala Gly Asp Thr Asn Pro Ile Arg Lys Asn Thr Pro Ser Tyr Ser Leu 
305                 310                 315                 320 


Pro Ala Met Leu Ala Ser Ser Phe Tyr Lys Asp Ser Tyr Leu Ala Tyr 
                325                 330                 335     


Glu Tyr Glu Arg Lys Pro Asn Ile Glu Arg His Cys Leu Ile Phe Asp 
            340                 345                 350         


Ile Leu Trp Arg Asp Leu Asp Leu Lys Ala Lys Ala Pro Asp Asp Leu 
        355                 360                 365             


Pro Leu Thr Arg Tyr Ser Gly Ser Pro Phe Gly Trp Met Ile Ala Arg 
    370                 375                 380                 


Thr Ala Trp Asp Glu Asn Ser Val Ile Ala Glu Met Lys Ile Asn Glu 
385                 390                 395                 400 


Gln Phe Val Gly Asn His Gln His Leu Asp Gly Gly Ser Phe Gln Leu 
                405                 410                 415     


Tyr Tyr Lys Gly Pro Leu Ala Ile Asp Ala Gly Ala Tyr Gln Gly Ser 
            420                 425                 430         


Ser Gly Gly Tyr Asn Ser Pro His Asn Lys Asn Phe Phe Lys Arg Thr 
        435                 440                 445             


Ile Ala His Asn Ser Leu Leu Val Tyr Asn Pro Asp Glu Lys Phe Ala 
    450                 455                 460                 


Cys Trp Asn Tyr Gly Gly Gly Gly Lys Thr Glu Phe Ala Ala Asn Asp 
465                 470                 475                 480 


Gly Gly Gln Arg Met Pro Gly Asp Arg Trp Glu Thr Cys Arg Ser Phe 
                485                 490                 495     


Lys Gln Leu Met Ser Lys Asp Tyr Thr Thr Gly Lys Ala Leu Ala His 
            500                 505                 510         


Gly Phe Gly Pro Asp Ala Cys Lys Pro Asp Tyr Ser Tyr Leu Lys Gly 
        515                 520                 525             


Asp Ile Thr Gln Ala Tyr Thr Asp Lys Val Lys Glu Ala Lys Arg Ser 
    530                 535                 540                 


Phe Val Phe Leu Asn Leu His Ser Thr Glu Val Pro Gly Ala Leu Ile 
545                 550                 555                 560 


Val Phe Asp Lys Val Val Ser Ser Asp Pro Gln Phe Lys Lys Phe Trp 
                565                 570                 575     


Leu Leu His Ser Ile Glu Glu Pro Val Ile Glu Gly Asp Arg Phe Ile 
            580                 585                 590         


Ile Arg Arg Thr Lys Asn Gly Asp Thr Gly Met Leu Gln Asn Gln Val 
        595                 600                 605             


Leu Leu Pro Glu Ala Gly Asn Ala Gln Ile Glu Lys Val Gly Gly Lys 
    610                 615                 620                 


Gly Lys Glu Phe Trp Val Phe Gly Thr Asn Tyr Pro Asn Asp Ala Leu 
625                 630                 635                 640 


Pro Asn Arg Pro Asp Asp Ala Asn Glu Arg Gly Ala Trp Arg Val Glu 
                645                 650                 655     


Val Ser Pro Ala Val Pro Ala Ala Glu Asn Tyr Phe Leu Asn Val Ile 
            660                 665                 670         


Gln Val Ala Asp Asn Thr Cys Lys Arg Met Asn Asp Val Lys Arg Ile 
        675                 680                 685             


Asp Ala Gly Lys Val Val Gly Val Gln Ile Ala Asp Arg Ile Val Thr 
    690                 695                 700                 


Phe Ser Lys Asn Ser Leu Pro Leu Ser Gly Lys Ile Asp Met Lys Val 
705                 710                 715                 720 


Asp Gly Asn Thr Ser Met Lys Phe Val Ile Thr Asp Leu Ile Pro Gly 
                725                 730                 735     


Thr Trp Gln Ile Lys Lys Asp Gly Lys Val Tyr Ile Pro Ala Met Glu 
            740                 745                 750         


Val Arg Ser Asp Asp Gly Ile Leu Ser Phe Glu Gly Thr Ala Gly His 
        755                 760                 765             


Tyr Glu Phe Leu Arg 
    770             


<210>  40
<211>  666
<212>  PRT
<213>  Bacteroides eggerthii

<400>  40

Met Lys Ile Met Lys Phe Ile Leu Ser Val Phe Leu Leu Thr Ile Ala 
1               5                   10                  15      


Ile Ile Ala Asp Ala Gln Gln Leu Arg Lys Glu Ala Phe Asp Leu Leu 
            20                  25                  30          


Asn Leu Asp Tyr Pro Gly Leu Glu Lys Val Lys Thr Ala Cys Ser Arg 
        35                  40                  45              


Gln Gln Trp Glu Glu Ala Ala Gln Glu Leu Leu Ala Tyr Tyr Arg Asn 
    50                  55                  60                  


Arg Thr Asp Ile Ala His Pro Asp Ile Asp Leu Lys Asn Leu Ala Ile 
65                  70                  75                  80  


Ser Lys Glu Glu Gln Lys Trp Ala Asp Asp Ala Met Asp His Thr Phe 
                85                  90                  95      


Phe Val His Lys Gly Tyr Gln Pro Ser Tyr Asn Tyr Gly Lys Asp Ile 
            100                 105                 110         


Asn Trp Glu Tyr Trp Pro Val Lys Asp Asn Glu Leu Arg Trp Gln Leu 
        115                 120                 125             


His Arg His Lys Trp Phe Thr Pro Met Gly Lys Ala Tyr Arg Ile Ser 
    130                 135                 140                 


Gly Asp Glu Lys Tyr Ala Lys Glu Trp Ala Phe Gln Tyr Ile Asp Trp 
145                 150                 155                 160 


Ile Lys Lys Asn Pro Leu Val Lys Met Glu Lys Glu Asn Phe Glu Leu 
                165                 170                 175     


Val Ser Ala Gly Glu Val Lys Glu Asp Ala Asp Asn Val His Phe Ala 
            180                 185                 190         


Trp Arg Gln Leu Glu Val Ser Asn Arg Leu Gln Asp Gln Thr Cys Gln 
        195                 200                 205             


Phe Leu Leu Phe Cys Pro Ala Glu Ala Phe Thr Pro Glu Phe Leu Thr 
    210                 215                 220                 


Glu Phe Leu Val Asn Tyr His Arg His Gly Ala Tyr Leu Phe Lys Asn 
225                 230                 235                 240 


Tyr Ser Ala Glu Gly Asn His Leu Leu Phe Glu Ala Gln Arg Met Val 
                245                 250                 255     


Tyr Ala Gly Val Phe Phe Pro Glu Phe Lys Asp Ala Ala Thr Trp Arg 
            260                 265                 270         


Glu Ser Gly Ile Asn Ile Leu Asn Arg Glu Ile Lys Lys Gln Val Tyr 
        275                 280                 285             


Asp Asp Gly Gly Gln Tyr Glu Leu Asp Pro His Tyr His Leu Ala Ala 
    290                 295                 300                 


Ile Asn Ile Phe Cys Lys Ala Leu Arg Met Ala Asp Cys Asn Gly Phe 
305                 310                 315                 320 


Arg Asn Glu Phe Pro Ala Glu Tyr Leu Asp Thr Val Lys Lys Met Ile 
                325                 330                 335     


Glu Phe Tyr Thr Asn Ile Cys Phe Pro Asp Tyr Thr Asn Pro Cys Phe 
            340                 345                 350         


Ser Asp Ala Lys Leu Gly Asp Tyr Lys Ser Glu Leu Ala Asn Tyr Arg 
        355                 360                 365             


Asp Trp Val Thr Leu Phe Pro Asp Ser Glu Trp Ile Arg Tyr Tyr Ala 
    370                 375                 380                 


Thr Glu Gly Arg Glu Gly Ala Pro Leu Pro Tyr Leu Ser His Gly Ser 
385                 390                 395                 400 


Leu Ala Ser Gly Phe Phe Thr Phe Arg Ser Gly Trp Lys Lys Asp Ala 
                405                 410                 415     


Ala Val Val Val Val Lys Ala Gly Pro Lys Gly Glu Trp His Cys Gln 
            420                 425                 430         


Pro Asp Asn Gly Thr Phe Glu Phe Trp Phe Asn Gly Lys Asn Leu Phe 
        435                 440                 445             


Pro Asp Ser Gly Ala Tyr Val Tyr Ala Gly Ser Asp Glu Val Met Lys 
    450                 455                 460                 


Leu Arg Asn Trp Phe Arg Gln Thr Arg Val His Asn Thr Leu Thr Leu 
465                 470                 475                 480 


Asp Gly Arg Asn Phe Glu Thr Thr Gln Ser Val Thr Lys Leu Trp Gln 
                485                 490                 495     


Pro Glu Gly Arg Glu Gln Ile Leu Val Thr Glu Asn Pro Ser Tyr Gln 
            500                 505                 510         


Gly Leu Lys His Arg Arg Thr Val Phe Phe Val Glu Gln Thr Tyr Tyr 
        515                 520                 525             


Val Ile Val Asp Glu Ala Val Gly Asp Ala Lys Gly Thr Val Asn Leu 
    530                 535                 540                 


Asn Tyr His Phe Cys Glu Gly Thr Val Asn Val Asp Val Lys Lys Asn 
545                 550                 555                 560 


Met Ala Thr Thr Ala Tyr Ala Gly Pro Ser Asn Val Lys Leu Gln Cys 
                565                 570                 575     


Phe Pro Glu Lys Lys Ala Ser Leu Lys Lys Glu Glu Gly Trp Arg Ser 
            580                 585                 590         


Ile Ala Tyr Arg Gln Arg Val Pro Arg Thr Ser Leu Ser Phe Asp Ile 
        595                 600                 605             


His Lys Asp Asp Ala Glu Ala Val Arg Tyr Ile Thr Val Ile Tyr Pro 
    610                 615                 620                 


Val Lys Asp Ala Ala Ser Tyr Pro Val Leu Lys Ala Lys Phe Leu Asn 
625                 630                 635                 640 


Lys Asp Phe Asp Glu Lys Gly Val Lys Val Glu Val Ser Val Asn Gly 
                645                 650                 655     


Val Ala Arg Gln Leu Met Ser Gln Leu Lys 
            660                 665     


<210>  41
<211>  617
<212>  PRT
<213>  Homo sapiens

<400>  41

Met Arg Cys Leu Ala Ala Arg Val Asn Tyr Lys Thr Leu Ile Ile Ile 
1               5                   10                  15      


Cys Ala Leu Phe Thr Leu Val Thr Val Leu Leu Trp Asn Lys Cys Ser 
            20                  25                  30          


Ser Asp Lys Ala Ile Gln Phe Pro Arg Arg Ser Ser Ser Gly Phe Arg 
        35                  40                  45              


Val Asp Gly Phe Glu Lys Arg Ala Ala Ala Ser Glu Ser Asn Asn Tyr 
    50                  55                  60                  


Met Asn His Val Ala Lys Gln Gln Ser Glu Glu Ala Phe Pro Gln Glu 
65                  70                  75                  80  


Gln Gln Lys Ala Pro Pro Val Val Gly Gly Phe Asn Ser Asn Val Gly 
                85                  90                  95      


Ser Lys Val Leu Gly Leu Lys Tyr Glu Glu Ile Asp Cys Leu Ile Asn 
            100                 105                 110         


Asp Glu His Thr Ile Lys Gly Arg Arg Glu Gly Asn Glu Val Phe Leu 
        115                 120                 125             


Pro Phe Thr Trp Val Glu Lys Tyr Phe Asp Val Tyr Gly Lys Val Val 
    130                 135                 140                 


Gln Tyr Asp Gly Tyr Asp Arg Phe Glu Phe Ser His Ser Tyr Ser Lys 
145                 150                 155                 160 


Val Tyr Ala Gln Arg Ala Pro Tyr His Pro Asp Gly Val Phe Met Ser 
                165                 170                 175     


Phe Glu Gly Tyr Asn Val Glu Val Arg Asp Arg Val Lys Cys Ile Ser 
            180                 185                 190         


Gly Val Glu Gly Val Pro Leu Ser Thr Gln Trp Gly Pro Gln Gly Tyr 
        195                 200                 205             


Phe Tyr Pro Ile Gln Ile Ala Gln Tyr Gly Leu Ser His Tyr Ser Lys 
    210                 215                 220                 


Asn Leu Thr Glu Lys Pro Pro His Ile Glu Val Tyr Glu Thr Ala Glu 
225                 230                 235                 240 


Asp Arg Asp Lys Asn Lys Pro Asn Asp Trp Thr Val Pro Lys Gly Cys 
                245                 250                 255     


Phe Met Ala Asn Val Ala Asp Lys Ser Arg Phe Thr Asn Val Lys Gln 
            260                 265                 270         


Phe Ile Ala Pro Glu Thr Ser Glu Gly Val Ser Leu Gln Leu Gly Asn 
        275                 280                 285             


Thr Lys Asp Phe Ile Ile Ser Phe Asp Leu Lys Phe Leu Thr Asn Gly 
    290                 295                 300                 


Ser Val Ser Val Val Leu Glu Thr Thr Glu Lys Asn Gln Leu Phe Thr 
305                 310                 315                 320 


Ile His Tyr Val Ser Asn Ala Gln Leu Ile Ala Phe Lys Glu Arg Asp 
                325                 330                 335     


Ile Tyr Tyr Gly Ile Gly Pro Arg Thr Ser Trp Ser Thr Val Thr Arg 
            340                 345                 350         


Asp Leu Val Thr Asp Leu Arg Lys Gly Val Gly Leu Ser Asn Thr Lys 
        355                 360                 365             


Ala Val Lys Pro Thr Lys Ile Met Pro Lys Lys Val Val Arg Leu Ile 
    370                 375                 380                 


Ala Lys Gly Lys Gly Phe Leu Asp Asn Ile Thr Ile Ser Thr Thr Ala 
385                 390                 395                 400 


His Met Ala Ala Phe Phe Ala Ala Ser Asp Trp Leu Val Arg Asn Gln 
                405                 410                 415     


Asp Glu Lys Gly Gly Trp Pro Ile Met Val Thr Arg Lys Leu Gly Glu 
            420                 425                 430         


Gly Phe Lys Ser Leu Glu Pro Gly Trp Tyr Ser Ala Met Ala Gln Gly 
        435                 440                 445             


Gln Ala Ile Ser Thr Leu Val Arg Ala Tyr Leu Leu Thr Lys Asp His 
    450                 455                 460                 


Ile Phe Leu Asn Ser Ala Leu Arg Ala Thr Ala Pro Tyr Lys Phe Leu 
465                 470                 475                 480 


Ser Glu Gln His Gly Val Lys Ala Val Phe Met Asn Lys His Asp Trp 
                485                 490                 495     


Tyr Glu Glu Tyr Pro Thr Thr Pro Ser Ser Phe Val Leu Asn Gly Phe 
            500                 505                 510         


Met Tyr Ser Leu Ile Gly Leu Tyr Asp Leu Lys Glu Thr Ala Gly Glu 
        515                 520                 525             


Lys Leu Gly Lys Glu Ala Arg Ser Leu Tyr Glu Arg Gly Met Glu Ser 
    530                 535                 540                 


Leu Lys Ala Met Leu Pro Leu Tyr Asp Thr Gly Ser Gly Thr Ile Tyr 
545                 550                 555                 560 


Asp Leu Arg His Phe Met Leu Gly Ile Ala Pro Asn Leu Ala Arg Trp 
                565                 570                 575     


Asp Tyr His Thr Thr His Ile Asn Gln Leu Gln Leu Leu Ser Thr Ile 
            580                 585                 590         


Asp Glu Ser Pro Val Phe Lys Glu Phe Val Lys Arg Trp Lys Ser Tyr 
        595                 600                 605             


Leu Lys Gly Ser Arg Ala Lys His Asn 
    610                 615         


