                         SEQUENCE LISTING

<110>  The United States of America, as represented by the 
       Secretary, Department of Health and Human Services
       Allotropic Tech, LLC
 
<120>  METHODS TO PRODUCE VERY LONG CHAIN FATTY ACIDS

<130>  4239-104579-02

<150>  US 63/051,520
<151>  2020-07-14

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  295
<212>  PRT
<213>  Homo sapiens

<400>  1

Glu His Leu Lys Ala Phe Asp Asp Glu Ile Asn Ala Phe Leu Asp Asn 
1               5                   10                  15      


Met Phe Gly Pro Arg Asp Ser Arg Val Arg Gly Trp Phe Met Leu Asp 
            20                  25                  30          


Ser Tyr Leu Pro Thr Phe Phe Leu Thr Val Met Tyr Leu Leu Ser Ile 
        35                  40                  45              


Trp Leu Gly Asn Lys Tyr Met Lys Asn Arg Pro Ala Leu Ser Leu Arg 
    50                  55                  60                  


Gly Ile Leu Thr Leu Tyr Asn Leu Gly Ile Thr Leu Leu Ser Ala Tyr 
65                  70                  75                  80  


Met Leu Ala Glu Leu Ile Leu Ser Thr Trp Glu Gly Gly Tyr Asn Leu 
                85                  90                  95      


Gln Cys Gln Asp Leu Thr Ser Ala Gly Glu Ala Asp Ile Arg Val Ala 
            100                 105                 110         


Lys Val Leu Trp Trp Tyr Tyr Phe Ser Lys Ser Val Glu Phe Leu Asp 
        115                 120                 125             


Thr Ile Phe Phe Val Leu Arg Lys Lys Thr Ser Gln Ile Thr Phe Leu 
    130                 135                 140                 


His Val Tyr His His Ala Ser Met Phe Asn Ile Trp Trp Cys Val Leu 
145                 150                 155                 160 


Asn Trp Ile Pro Cys Gly Gln Ser Phe Phe Gly Pro Thr Leu Asn Ser 
                165                 170                 175     


Phe Ile His Ile Leu Met Tyr Ser Tyr Tyr Gly Leu Ser Val Phe Pro 
            180                 185                 190         


Ser Met His Lys Tyr Leu Trp Trp Lys Lys Tyr Leu Thr Gln Ala Gln 
        195                 200                 205             


Leu Val Gln Phe Val Leu Thr Ile Thr His Thr Met Ser Ala Val Val 
    210                 215                 220                 


Lys Pro Cys Gly Phe Pro Phe Gly Cys Leu Ile Phe Gln Ser Ser Tyr 
225                 230                 235                 240 


Met Leu Thr Leu Val Ile Leu Phe Leu Asn Phe Tyr Val Gln Thr Tyr 
                245                 250                 255     


Arg Lys Lys Pro Met Lys Lys Asp Met Gln Glu Pro Pro Ala Gly Lys 
            260                 265                 270         


Glu Val Lys Asn Gly Phe Ser Lys Ala Tyr Phe Thr Ala Ala Asn Gly 
        275                 280                 285             


Val Met Asn Lys Lys Ala Gln 
    290                 295 


<210>  2
<211>  888
<212>  DNA
<213>  Homo sapiens

<400>  2
gaacatctga aggccttcga cgacgagatc aatgcctttc tggacaacat gttcggtcct       60

cgtgacagcc gtgtgagagg ctggttcatg ctggactctt atctgcccac tttcttcctc      120

accgtgatgt atctgctgag catctggctg ggtaacaagt acatgaagaa ccgtcccgct      180

ctgtctctga gaggcattct gactctctac aatctgggca tcactctgct cagcgcttac      240

atgctggctg agctgatcct cagcacttgg gagggtggct acaatctgca gtgccaagac      300

ctcactagcg ccggtgaagc cgacatcaga gtcgccaagg tgctgtggtg gtactacttc      360

agcaagagcg tcgagtttct ggacaccatc ttctttgtcc tcagaaagaa aaccagccag      420

attaccttcc tccacgtcta ccaccacgcc agcatgttca acatctggtg gtgcgtgctg      480

aactggattc cttgcggcca gtctttcttc ggccccactc tgaacagctt catccacatt      540

ctgatgtaca gctactacgg tctgtctgtc tttcccagca tgcacaagta tctgtggtgg      600

aagaagtacc tcacccaagc tcagctggtc caattcgtgc tgactatcac ccacaccatg      660

tctgctgtgg tgaagccttg cggtttcccc ttcggttgcc tcatcttcca gagctcttac      720

atgctgactc tggtcattct gttcctcaat ttctacgtgc agacctacag aaagaagcct      780

atgaagaagg atatgcaaga accccccgct ggcaaagaag tcaagaacgg cttcagcaag      840

gcctacttca ccgccgctaa cggtgtcatg aacaagaagg ctcagtaa                   888


<210>  3
<211>  313
<212>  PRT
<213>  Homo sapiens

<400>  3

Gly Leu Leu Asp Ser Glu Pro Gly Ser Val Leu Asn Val Val Ser Thr 
1               5                   10                  15      


Ala Leu Asn Asp Thr Val Glu Phe Tyr Arg Trp Thr Trp Ser Ile Ala 
            20                  25                  30          


Asp Lys Arg Val Glu Asn Trp Pro Leu Met Gln Ser Pro Trp Pro Thr 
        35                  40                  45              


Leu Ser Ile Ser Thr Leu Tyr Leu Leu Phe Val Trp Leu Gly Pro Lys 
    50                  55                  60                  


Trp Met Lys Asp Arg Glu Pro Phe Gln Met Arg Leu Val Leu Ile Ile 
65                  70                  75                  80  


Tyr Asn Phe Gly Met Val Leu Leu Asn Leu Phe Ile Phe Arg Glu Leu 
                85                  90                  95      


Phe Met Gly Ser Tyr Asn Ala Gly Tyr Ser Tyr Ile Cys Gln Ser Val 
            100                 105                 110         


Asp Tyr Ser Asn Asn Val His Glu Val Arg Ile Ala Ala Ala Leu Trp 
        115                 120                 125             


Trp Tyr Phe Val Ser Lys Gly Val Glu Tyr Leu Asp Thr Val Phe Phe 
    130                 135                 140                 


Ile Leu Arg Lys Lys Asn Asn Gln Val Ser Phe Leu His Val Tyr His 
145                 150                 155                 160 


His Cys Thr Met Phe Thr Leu Trp Trp Ile Gly Ile Lys Trp Val Ala 
                165                 170                 175     


Gly Gly Gln Ala Phe Phe Gly Ala Gln Leu Asn Ser Phe Ile His Val 
            180                 185                 190         


Ile Met Tyr Ser Tyr Tyr Gly Leu Thr Ala Phe Gly Pro Trp Ile Gln 
        195                 200                 205             


Lys Tyr Leu Trp Trp Lys Arg Tyr Leu Thr Met Leu Gln Leu Ile Gln 
    210                 215                 220                 


Phe His Val Thr Ile Gly His Thr Ala Leu Ser Leu Tyr Thr Asp Cys 
225                 230                 235                 240 


Pro Phe Pro Lys Trp Met His Trp Ala Leu Ile Ala Tyr Ala Ile Ser 
                245                 250                 255     


Phe Ile Phe Leu Phe Leu Asn Phe Tyr Ile Arg Thr Tyr Lys Glu Pro 
            260                 265                 270         


Lys Lys Pro Lys Ala Gly Lys Thr Ala Met Asn Gly Ile Ser Ala Asn 
        275                 280                 285             


Gly Val Ser Lys Ser Glu Lys Gln Leu Met Ile Glu Asn Gly Lys Lys 
    290                 295                 300                 


Gln Lys Asn Gly Lys Ala Lys Gly Asp 
305                 310             


<210>  4
<211>  941
<212>  DNA
<213>  Homo sapiens

<400>  4
ggtctgctgg attctgaacc cggttctgtc ctcaacgtgg tctctaccgc tctgaacgac       60

accgtcgagt tctaccgttg gacttggtct atcgccgaca aaagagtgga gaactggcct      120

ctcatgcagt ctccttggcc tactctgagc atcagcactc tgtatctgct gttcgtctgg      180

ctgggtccta agtggatgaa ggaccgtgaa cccttccaga tgcgtctcgt cctcatcatc      240

tacaacttcg gcatggtgct cctcaatctg ttcatcttcc gtgagctgtt catgggcagc      300

tacaatgctg gctacagcta catttgccag agcgtcgact acagcaacaa cgtccacgag      360

gtcagatcgc tgccgctctg tggtggtact tcgtcagcaa gggtgtcgaa tatctggaca      420

ccgtgttctt catcctcaga aagaagaaca accaagtcag ctttctgcac gtgtatcacc      480

actgcaccat gttcactctg tggtggatcg gtatcaagtg ggtcgccggt ggtcaagcct      540

ttttcggtgc tcagctcaac agcttcatcc acgtgatcat gtacagctat tacggcctca      600

ccgccttcgg cccttggatt cagaagtacc tctggtggaa gcgttatctg accatgctgc      660

agctgatcca gttccacgtg accattggcc acactgccct ctctctgtat actgactgcc      720

ctttccccaa gtggatgcac tgggctctga tcgcctacgc catcagcttc atctttctgt      780

tcctcaactt ctatatccgt acctacaagg aacctaagaa gcccaaggcc ggcaaaactg      840

ccatgaacgg catcagcgct aacggcgtca gcaagagcga gaagcagctc atgatcgaga      900

acggcaagaa gcaaaaaaac ggcaaggcca agggcgacta a                          941


<210>  5
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bombyxin signal peptide

<400>  5

Met Lys Ile Leu Leu Ala Ile Ala Leu Met Leu Ser Thr Val Met Trp 
1               5                   10                  15      


Val Ser Thr 
            


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG tag

<400>  6

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


