<110>    LG CHEM, LTD.

<120>    Composition for preventing or treating osteoarthritis comprising
         mesenchymal stem cell expressing tumor necrosis factor-inducible
         gene 6

<130>    OPP20212366KR

<150>    KR 10-2020-0089148
<151>    2020-07-17

<160>    10

<170>    KoPatentIn 3.0

<210>    1
<211>    277
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic_TSG-6 amino acid sequence


<400>    1
Met Ile Ile Leu Ile Tyr Leu Phe Leu Leu Leu Trp Glu Asp Thr Gln
  1               5                  10                  15 

Gly Trp Gly Phe Lys Asp Gly Ile Phe His Asn Ser Ile Trp Leu Glu
             20                  25                  30 

Arg Ala Ala Gly Val Tyr His Arg Glu Ala Arg Ser Gly Lys Tyr Lys
         35                  40                  45 

Leu Thr Tyr Ala Glu Ala Lys Ala Val Cys Glu Phe Glu Gly Gly His
     50                  55                  60 

Leu Ala Thr Tyr Lys Gln Leu Glu Ala Ala Arg Lys Ile Gly Phe His
 65                  70                  75                  80 

Val Cys Ala Ala Gly Trp Met Ala Lys Gly Arg Val Gly Tyr Pro Ile
                 85                  90                  95 

Val Lys Pro Gly Pro Asn Cys Gly Phe Gly Lys Thr Gly Ile Ile Asp
            100                 105                 110 

Tyr Gly Ile Arg Leu Asn Arg Ser Glu Arg Trp Asp Ala Tyr Cys Tyr
        115                 120                 125 

Asn Pro His Ala Lys Glu Cys Gly Gly Val Phe Thr Asp Pro Lys Gln
    130                 135                 140 

Ile Phe Lys Ser Pro Gly Phe Pro Asn Glu Tyr Glu Asp Asn Gln Ile
145                 150                 155                 160 

Cys Tyr Trp His Ile Arg Leu Lys Tyr Gly Gln Arg Ile His Leu Ser
                165                 170                 175 

Phe Leu Asp Phe Asp Leu Glu Asp Asp Pro Gly Cys Leu Ala Asp Tyr
            180                 185                 190 

Val Glu Ile Tyr Asp Ser Tyr Asp Asp Val His Gly Phe Val Gly Arg
        195                 200                 205 

Tyr Cys Gly Asp Glu Leu Pro Asp Asp Ile Ile Ser Thr Gly Asn Val
    210                 215                 220 

Met Thr Leu Lys Phe Leu Ser Asp Ala Ser Val Thr Ala Gly Gly Phe
225                 230                 235                 240 

Gln Ile Lys Tyr Val Ala Met Asp Pro Val Ser Lys Ser Ser Gln Gly
                245                 250                 255 

Lys Asn Thr Ser Thr Thr Ser Thr Gly Asn Lys Asn Phe Leu Ala Gly
            260                 265                 270 

Arg Phe Ser His Leu
        275        


<210>    2
<211>    1422
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_TSG-6 gene


<400>    2
agtcacattt cagccactgc tctgagaatt tgtgagcagc ccctaacagg ctgttacttc     60

actacaactg acgatatgat catcttaatt tacttatttc tcttgctatg ggaagacact    120

caaggatggg gattcaagga tggaattttt cataactcca tatggcttga acgagcagcc    180

ggtgtgtacc acagagaagc acggtctggc aaatacaagc tcacctacgc agaagctaag    240

gcggtgtgtg aatttgaagg cggccatctc gcaacttaca agcagctaga ggcagccaga    300

aaaattggat ttcatgtctg tgctgctgga tggatggcta agggcagagt tggatacccc    360

attgtgaagc cagggcccaa ctgtggattt ggaaaaactg gcattattga ttatggaatc    420

cgtctcaata ggagtgaaag atgggatgcc tattgctaca acccacacgc aaaggagtgt    480

ggtggcgtct ttacagatcc aaagcaaatt tttaaatctc caggcttccc aaatgagtac    540

gaagataacc aaatctgcta ctggcacatt agactcaagt atggtcagcg tattcacctg    600

agttttttag attttgacct tgaagatgac ccaggttgct tggctgatta tgttgaaata    660

tatgacagtt acgatgatgt ccatggcttt gtgggaagat actgtggaga tgagcttcca    720

gatgacatca tcagtacagg aaatgtcatg accttgaagt ttctaagtga tgcttcagtg    780

acagctggag gtttccaaat caaatatgtt gcaatggatc ctgtatccaa atccagtcaa    840

ggaaaaaata caagtactac ttctactgga aataaaaact ttttagctgg aagatttagc    900

cacttataaa aaaaaaaaaa aggatgatca aaacacacag tgtttatgtt ggaatctttt    960

ggaactcctt tgatctcact gttattatta acatttattt attatttttc taaatgtgaa   1020

agcaatacat aatttaggga aaattggaaa atataggaaa ctttaaacga gaaaatgaaa   1080

cctctcataa tcccactgca tagaaataac aagcgttaac attttcatat ttttttcttt   1140

cagtcatttt tctatttgtg gtatatgtat atatgtacct atatgtattt gcatttgaaa   1200

ttttggaatc ctgctctatg tacagttttg tattatactt tttaaatctt gaactttata   1260

aacattttct gaaatcattg attattctac aaaaacatga ttttaaacag ctgtaaaata   1320

ttctatgata tgaatgtttt atgcattatt taagcctgtc tctattgttg gaatttcagg   1380

tcattttcat aaatattgtt gcaataaata tccttgaaca ca                      1422


<210>    3
<211>    834
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_TSG-6 cDNA


<400>    3
atgatcatct taatttactt atttctcttg ctatgggaag acactcaagg atggggattc     60

aaggatggaa tttttcataa ctccatatgg cttgaacgag cagccggtgt gtaccacaga    120

gaagcacggt ctggcaaata caagctcacc tacgcagaag ctaaggcggt gtgtgaattt    180

gaaggcggcc atctcgcaac ttacaagcag ctagaggcag ccagaaaaat tggatttcat    240

gtctgtgctg ctggatggat ggctaagggc agagttggat accccattgt gaagccaggg    300

cccaactgtg gatttggaaa aactggcatt attgattatg gaatccgtct caataggagt    360

gaaagatggg atgcctattg ctacaaccca cacgcaaagg agtgtggtgg cgtctttaca    420

gatccaaagc aaatttttaa atctccaggc ttcccaaatg agtacgaaga taaccaaatc    480

tgctactggc acattagact caagtatggt cagcgtattc acctgagttt tttagatttt    540

gaccttgaag atgacccagg ttgcttggct gattatgttg aaatatatga cagttacgat    600

gatgtccatg gctttgtggg aagatactgt ggagatgagc ttccagatga catcatcagt    660

acaggaaatg tcatgacctt gaagtttcta agtgatgctt cagtgacagc tggaggtttc    720

caaatcaaat atgttgcaat ggatcctgta tccaaatcca gtcaaggaaa aaatacaagt    780

actacttcta ctggaaataa aaacttttta gctggaagat ttagccactt ataa          834


<210>    4
<211>    834
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_TSG-6 cDNA_codon optimization


<400>    4
atgattatcc tgatctacct gttcctgctg ctgtgggaag atacccaagg ctggggcttc     60

aaggacggca tcttccacaa cagcatctgg ctggaaagag ccgctggcgt ctaccacaga    120

gaagccagaa gcggcaagta caagctgacc tacgccgagg ccaaagccgt gtgcgaattt    180

gaaggcggac acctggccac ctacaagcag ctggaagccg ccagaaagat cggcttccat    240

gtgtgcgctg ccggctggat ggctaagggc agagtgggat accccatcgt gaagcccgga    300

cctaattgcg gctttggaaa gaccggcatc atcgactacg gcatccggct gaacagatcc    360

gagagatggg acgcctactg ctacaaccct cacgccaaag aatgtggcgg cgtgttcaca    420

gaccccaagc agatctttaa gagccctggc ttccccaacg agtacgagga caaccagatc    480

tgctactggc acatcagact gaagtacggc cagcggatcc acctgagctt tctggacttc    540

gacctggaag atgaccctgg ctgcctggcc gactacgtgg aaatctacga cagctacgac    600

gacgtgcacg gcttcgtggg cagatactgc ggagatgagc tgcccgacga catcatcagc    660

accggcaacg tgatgactct gaagttcctg agcgacgcct ctgtgacagc cggcggattc    720

cagatcaaat acgtggccat ggatcccgtg tccaagtcca gccagggcaa gaataccagc    780

accacctcta ccggcaacaa gaacttcctg gccggcagat tcagccacct gtaa          834



<210>    5
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_TSG-6 forward primer


<400>    5
tggatggcta agggcagag                                                  19



<210>    6
<211>    17
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_TSG-6 reverse primer


<400>    6
gcgtgtgggt tgtagca                                                    17



<210>    7
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_GAPDH forward primer


<400>    7
gtctcctctg acttcaacag cg                                              22



<210>    8
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_GAPDH reverse primer


<400>    8
accaccctgt tgctgtagcc aa                                              22



<210>    9
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_collagen II forward primer


<400>    9
ctcaagtcgc tgaacaacca                                                 20



<210>    10
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic_collagen II reverse primer


<400>    10
gtctccgctc ttccactctg                                                 20