               SEQUENCE LISTING

<110> RIBOXX GMBH
      TRENZYME GMBH
      TER MEULEN

<120> Post-exposure vaccination against viral respiratory infections

<130> R 0350 WO

<140> EPPCT/2021/069673
<141> 2021-07-14

<150> EP20185962.6
<151> 2020-07-15
<150> EP20185839.6
<151> 2020-07-14

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 368
<212> PRT
<213> Artificial Sequence


<220> 
<223> HA1 with C-terminal His-tag and secretion signal

<400> 1
Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      
Val Gln Ala Met Asp Thr Ile Cys Val Gly Tyr His Ala Asn Asn Ser 
            20                  25                  30          
Thr Asp Thr Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His 
        35                  40                  45              
Ser Val Asn Leu Leu Glu Asn Ser His Asn Gly Lys Leu Cys Ser Leu 
    50                  55                  60                  
Asn Gly Lys Ile Pro Leu Gln Leu Gly Asn Cys Asn Val Ala Gly Trp 
65                  70                  75                  80  
Ile Leu Gly Asn Pro Lys Cys Asp Leu Leu Leu Thr Ala Asn Ser Trp 
                85                  90                  95      
Ser Tyr Ile Ile Glu Thr Ser Asn Ser Lys Asn Gly Ala Cys Tyr Pro 
            100                 105                 110         
Gly Glu Phe Ala Asp Tyr Glu Glu Leu Lys Glu Gln Leu Ser Thr Val 
        115                 120                 125             
Ser Ser Phe Glu Arg Phe Glu Ile Phe Pro Lys Ala Thr Ser Trp Pro 
    130                 135                 140                 
Asn His Asp Thr Thr Arg Gly Thr Thr Val Ala Cys Ser His Ser Gly 
145                 150                 155                 160 
Ala Asn Ser Phe Tyr Arg Asn Leu Leu Trp Ile Val Lys Lys Gly Asn 
                165                 170                 175     
Ser Tyr Pro Lys Leu Ser Lys Ser Tyr Thr Asn Asn Lys Gly Lys Glu 
            180                 185                 190         
Val Leu Val Ile Trp Gly Val His His Pro Pro Thr Asp Ser Asp Gln 
        195                 200                 205             
Gln Thr Leu Tyr Gln Asn Asn His Thr Tyr Val Ser Val Gly Ser Ser 
    210                 215                 220                 
Lys Tyr Tyr Lys Arg Phe Thr Pro Glu Ile Val Ala Arg Pro Lys Val 
225                 230                 235                 240 
Arg Glu Gln Ala Gly Arg Met Asn Tyr Tyr Trp Thr Leu Leu Asp Gln 
                245                 250                 255     
Gly Asp Thr Ile Thr Phe Glu Ala Thr Gly Asn Leu Ile Ala Pro Trp 
            260                 265                 270         
His Ala Phe Ala Leu Lys Lys Gly Ser Ser Ser Gly Ile Met Arg Ser 
        275                 280                 285             
Asp Ala Gln Val His Asn Cys Thr Thr Lys Cys Gln Thr Pro His Gly 
    290                 295                 300                 
Ala Leu Lys Gly Asn Leu Pro Phe Gln Asn Val His Pro Val Thr Ile 
305                 310                 315                 320 
Gly Lys Cys Pro Lys Tyr Val Lys Ser Thr Gln Leu Arg Met Ala Thr 
                325                 330                 335     
Gly Leu Arg Asn Ile Pro Ser Ile Gln Ser Arg Gly Glu Asn Leu Tyr 
            340                 345                 350         
Phe Gln Gly Gly Gly Ser His His His His His His His His His His 
        355                 360                 365             



<210> 2
<211> 329
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> HA1 without His-tag and without secretion signal

<400> 2
Met Asp Thr Ile Cys Val Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 
1               5                   10                  15      
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
            20                  25                  30          
Leu Leu Glu Asn Ser His Asn Gly Lys Leu Cys Ser Leu Asn Gly Lys 
        35                  40                  45              
Ile Pro Leu Gln Leu Gly Asn Cys Asn Val Ala Gly Trp Ile Leu Gly 
    50                  55                  60                  
Asn Pro Lys Cys Asp Leu Leu Leu Thr Ala Asn Ser Trp Ser Tyr Ile 
65                  70                  75                  80  
Ile Glu Thr Ser Asn Ser Lys Asn Gly Ala Cys Tyr Pro Gly Glu Phe 
                85                  90                  95      
Ala Asp Tyr Glu Glu Leu Lys Glu Gln Leu Ser Thr Val Ser Ser Phe 
            100                 105                 110         
Glu Arg Phe Glu Ile Phe Pro Lys Ala Thr Ser Trp Pro Asn His Asp 
        115                 120                 125             
Thr Thr Arg Gly Thr Thr Val Ala Cys Ser His Ser Gly Ala Asn Ser 
    130                 135                 140                 
Phe Tyr Arg Asn Leu Leu Trp Ile Val Lys Lys Gly Asn Ser Tyr Pro 
145                 150                 155                 160 
Lys Leu Ser Lys Ser Tyr Thr Asn Asn Lys Gly Lys Glu Val Leu Val 
                165                 170                 175     
Ile Trp Gly Val His His Pro Pro Thr Asp Ser Asp Gln Gln Thr Leu 
            180                 185                 190         
Tyr Gln Asn Asn His Thr Tyr Val Ser Val Gly Ser Ser Lys Tyr Tyr 
        195                 200                 205             
Lys Arg Phe Thr Pro Glu Ile Val Ala Arg Pro Lys Val Arg Glu Gln 
    210                 215                 220                 
Ala Gly Arg Met Asn Tyr Tyr Trp Thr Leu Leu Asp Gln Gly Asp Thr 
225                 230                 235                 240 
Ile Thr Phe Glu Ala Thr Gly Asn Leu Ile Ala Pro Trp His Ala Phe 
                245                 250                 255     
Ala Leu Lys Lys Gly Ser Ser Ser Gly Ile Met Arg Ser Asp Ala Gln 
            260                 265                 270         
Val His Asn Cys Thr Thr Lys Cys Gln Thr Pro His Gly Ala Leu Lys 
        275                 280                 285             
Gly Asn Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Lys Cys 
    290                 295                 300                 
Pro Lys Tyr Val Lys Ser Thr Gln Leu Arg Met Ala Thr Gly Leu Arg 
305                 310                 315                 320 
Asn Ile Pro Ser Ile Gln Ser Arg Gly 
                325                 

<210> 3
<211> 501
<212> PRT
<213> Artificial Sequence


<220> 
<223> HA1-C-CPE with N-terminal His-tag and secretion signal

<400> 3
Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      
Val Gln Ala Met Gly His His His His His His His His His Glu Asn 
            20                  25                  30          
Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Met Asp Thr Ile Cys Val 
        35                  40                  45              
Gly Tyr His Ala Asn Asn Ser Thr Asp Thr Val Asp Thr Ile Leu Glu 
    50                  55                  60                  
Lys Asn Val Thr Val Thr His Ser Val Asn Leu Leu Glu Asn Ser His 
65                  70                  75                  80  
Asn Gly Lys Leu Cys Ser Leu Asn Gly Lys Ile Pro Leu Gln Leu Gly 
                85                  90                  95      
Asn Cys Asn Val Ala Gly Trp Ile Leu Gly Asn Pro Lys Cys Asp Leu 
            100                 105                 110         
Leu Leu Thr Ala Asn Ser Trp Ser Tyr Ile Ile Glu Thr Ser Asn Ser 
        115                 120                 125             
Lys Asn Gly Ala Cys Tyr Pro Gly Glu Phe Ala Asp Tyr Glu Glu Leu 
    130                 135                 140                 
Lys Glu Gln Leu Ser Thr Val Ser Ser Phe Glu Arg Phe Glu Ile Phe 
145                 150                 155                 160 
Pro Lys Ala Thr Ser Trp Pro Asn His Asp Thr Thr Arg Gly Thr Thr 
                165                 170                 175     
Val Ala Cys Ser His Ser Gly Ala Asn Ser Phe Tyr Arg Asn Leu Leu 
            180                 185                 190         
Trp Ile Val Lys Lys Gly Asn Ser Tyr Pro Lys Leu Ser Lys Ser Tyr 
        195                 200                 205             
Thr Asn Asn Lys Gly Lys Glu Val Leu Val Ile Trp Gly Val His His 
    210                 215                 220                 
Pro Pro Thr Asp Ser Asp Gln Gln Thr Leu Tyr Gln Asn Asn His Thr 
225                 230                 235                 240 
Tyr Val Ser Val Gly Ser Ser Lys Tyr Tyr Lys Arg Phe Thr Pro Glu 
                245                 250                 255     
Ile Val Ala Arg Pro Lys Val Arg Glu Gln Ala Gly Arg Met Asn Tyr 
            260                 265                 270         
Tyr Trp Thr Leu Leu Asp Gln Gly Asp Thr Ile Thr Phe Glu Ala Thr 
        275                 280                 285             
Gly Asn Leu Ile Ala Pro Trp His Ala Phe Ala Leu Lys Lys Gly Ser 
    290                 295                 300                 
Ser Ser Gly Ile Met Arg Ser Asp Ala Gln Val His Asn Cys Thr Thr 
305                 310                 315                 320 
Lys Cys Gln Thr Pro His Gly Ala Leu Lys Gly Asn Leu Pro Phe Gln 
                325                 330                 335     
Asn Val His Pro Val Thr Ile Gly Lys Cys Pro Lys Tyr Val Lys Ser 
            340                 345                 350         
Thr Gln Leu Arg Met Ala Thr Gly Leu Arg Asn Ile Pro Ser Ile Gln 
        355                 360                 365             
Ser Arg Gly Gly Gly Gly Ser Asp Ile Glu Lys Glu Ile Leu Asp Leu 
    370                 375                 380                 
Ala Ala Ala Thr Glu Arg Leu Asn Leu Thr Asp Ala Leu Asn Ser Asn 
385                 390                 395                 400 
Pro Ala Gly Asn Leu Tyr Asp Trp Arg Ser Ser Asn Ser Tyr Pro Trp 
                405                 410                 415     
Thr Gln Lys Leu Asn Leu His Leu Thr Ile Thr Ala Thr Gly Gln Lys 
            420                 425                 430         
Tyr Arg Ile Leu Ala Ser Lys Ile Val Asp Phe Asn Ile Tyr Ser Asn 
        435                 440                 445             
Asn Phe Asn Asn Leu Val Lys Leu Glu Gln Ser Leu Gly Asp Gly Val 
    450                 455                 460                 
Lys Asp His Tyr Val Asp Ile Ser Leu Asp Ala Gly Gln Tyr Val Leu 
465                 470                 475                 480 
Val Met Lys Ala Asn Ser Ser Tyr Ser Gly Asn Tyr Pro Tyr Ser Ile 
                485                 490                 495     
Leu Phe Gln Lys Phe 
            500     

<210> 4
<211> 459
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> HA1-C-CPE without His-tag and without secretion signal

<400> 4
Met Asp Thr Ile Cys Val Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 
1               5                   10                  15      
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
            20                  25                  30          
Leu Leu Glu Asn Ser His Asn Gly Lys Leu Cys Ser Leu Asn Gly Lys 
        35                  40                  45              
Ile Pro Leu Gln Leu Gly Asn Cys Asn Val Ala Gly Trp Ile Leu Gly 
    50                  55                  60                  
Asn Pro Lys Cys Asp Leu Leu Leu Thr Ala Asn Ser Trp Ser Tyr Ile 
65                  70                  75                  80  
Ile Glu Thr Ser Asn Ser Lys Asn Gly Ala Cys Tyr Pro Gly Glu Phe 
                85                  90                  95      
Ala Asp Tyr Glu Glu Leu Lys Glu Gln Leu Ser Thr Val Ser Ser Phe 
            100                 105                 110         
Glu Arg Phe Glu Ile Phe Pro Lys Ala Thr Ser Trp Pro Asn His Asp 
        115                 120                 125             
Thr Thr Arg Gly Thr Thr Val Ala Cys Ser His Ser Gly Ala Asn Ser 
    130                 135                 140                 
Phe Tyr Arg Asn Leu Leu Trp Ile Val Lys Lys Gly Asn Ser Tyr Pro 
145                 150                 155                 160 
Lys Leu Ser Lys Ser Tyr Thr Asn Asn Lys Gly Lys Glu Val Leu Val 
                165                 170                 175     
Ile Trp Gly Val His His Pro Pro Thr Asp Ser Asp Gln Gln Thr Leu 
            180                 185                 190         
Tyr Gln Asn Asn His Thr Tyr Val Ser Val Gly Ser Ser Lys Tyr Tyr 
        195                 200                 205             
Lys Arg Phe Thr Pro Glu Ile Val Ala Arg Pro Lys Val Arg Glu Gln 
    210                 215                 220                 
Ala Gly Arg Met Asn Tyr Tyr Trp Thr Leu Leu Asp Gln Gly Asp Thr 
225                 230                 235                 240 
Ile Thr Phe Glu Ala Thr Gly Asn Leu Ile Ala Pro Trp His Ala Phe 
                245                 250                 255     
Ala Leu Lys Lys Gly Ser Ser Ser Gly Ile Met Arg Ser Asp Ala Gln 
            260                 265                 270         
Val His Asn Cys Thr Thr Lys Cys Gln Thr Pro His Gly Ala Leu Lys 
        275                 280                 285             
Gly Asn Leu Pro Phe Gln Asn Val His Pro Val Thr Ile Gly Lys Cys 
    290                 295                 300                 
Pro Lys Tyr Val Lys Ser Thr Gln Leu Arg Met Ala Thr Gly Leu Arg 
305                 310                 315                 320 
Asn Ile Pro Ser Ile Gln Ser Arg Gly Gly Gly Gly Ser Asp Ile Glu 
                325                 330                 335     
Lys Glu Ile Leu Asp Leu Ala Ala Ala Thr Glu Arg Leu Asn Leu Thr 
            340                 345                 350         
Asp Ala Leu Asn Ser Asn Pro Ala Gly Asn Leu Tyr Asp Trp Arg Ser 
        355                 360                 365             
Ser Asn Ser Tyr Pro Trp Thr Gln Lys Leu Asn Leu His Leu Thr Ile 
    370                 375                 380                 
Thr Ala Thr Gly Gln Lys Tyr Arg Ile Leu Ala Ser Lys Ile Val Asp 
385                 390                 395                 400 
Phe Asn Ile Tyr Ser Asn Asn Phe Asn Asn Leu Val Lys Leu Glu Gln 
                405                 410                 415     
Ser Leu Gly Asp Gly Val Lys Asp His Tyr Val Asp Ile Ser Leu Asp 
            420                 425                 430         
Ala Gly Gln Tyr Val Leu Val Met Lys Ala Asn Ser Ser Tyr Ser Gly 
        435                 440                 445             
Asn Tyr Pro Tyr Ser Ile Leu Phe Gln Lys Phe 
    450                 455                 

<210> 5
<211> 263
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 Spike S1 RBD with C-terminal His-tag and secretion
      signal

<400> 5
Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      
Val Gln Ala Met Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro 
            20                  25                  30          
Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
        35                  40                  45              
Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
    50                  55                  60                  
Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
65                  70                  75                  80  
Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
                85                  90                  95      
Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
            100                 105                 110         
Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
        115                 120                 125             
Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
    130                 135                 140                 
Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
145                 150                 155                 160 
Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
                165                 170                 175     
Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
            180                 185                 190         
Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
        195                 200                 205             
Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
    210                 215                 220                 
Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
225                 230                 235                 240 
Val Asn Phe Val Leu Val Pro Arg Gly Ser Ala Ala Ala Leu Glu His 
                245                 250                 255     
His His His His His His His 
            260             

<210> 6
<211> 224
<212> PRT
<213> SARS coronavirus


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> SARS-CoV-2 Spike S1 RBD

<400> 6
Met Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
1               5                   10                  15      
Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
            20                  25                  30          
Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
        35                  40                  45              
Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
    50                  55                  60                  
Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
65                  70                  75                  80  
Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
                85                  90                  95      
Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
            100                 105                 110         
Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
        115                 120                 125             
Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
    130                 135                 140                 
Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
145                 150                 155                 160 
Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
                165                 170                 175     
Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
            180                 185                 190         
Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
        195                 200                 205             
Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220                 



<210> 7
<211> 397
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 Spike S1 RBD-C-CPE with N-terminal His-tag and
      secretion signal

<400> 7
Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      
Val Gln Ala Met Gly His His His His His His His His His Glu Asn 
            20                  25                  30          
Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Met Arg Val Gln Pro Thr 
        35                  40                  45              
Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
    50                  55                  60                  
Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
65                  70                  75                  80  
Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
                85                  90                  95      
Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
            100                 105                 110         
Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
        115                 120                 125             
Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
    130                 135                 140                 
Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
145                 150                 155                 160 
Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
                165                 170                 175     
Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
            180                 185                 190         
Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
        195                 200                 205             
Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
    210                 215                 220                 
Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
225                 230                 235                 240 
Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
                245                 250                 255     
Asn Leu Val Lys Asn Lys Cys Val Asn Phe Gly Gly Gly Gly Ser Asp 
            260                 265                 270         
Ile Glu Lys Glu Ile Leu Asp Leu Ala Ala Ala Thr Glu Arg Leu Asn 
        275                 280                 285             
Leu Thr Asp Ala Leu Asn Ser Asn Pro Ala Gly Asn Leu Tyr Asp Trp 
    290                 295                 300                 
Arg Ser Ser Asn Ser Tyr Pro Trp Thr Gln Lys Leu Asn Leu His Leu 
305                 310                 315                 320 
Thr Ile Thr Ala Thr Gly Gln Lys Tyr Arg Ile Leu Ala Ser Lys Ile 
                325                 330                 335     
Val Asp Phe Asn Ile Tyr Ser Asn Asn Phe Asn Asn Leu Val Lys Leu 
            340                 345                 350         
Glu Gln Ser Leu Gly Asp Gly Val Lys Asp His Tyr Val Asp Ile Ser 
        355                 360                 365             
Leu Asp Ala Gly Gln Tyr Val Leu Val Met Lys Ala Asn Ser Ser Tyr 
    370                 375                 380                 
Ser Gly Asn Tyr Pro Tyr Ser Ile Leu Phe Gln Lys Phe 
385                 390                 395         

<210> 8
<211> 355
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> RBD-C-CPE without His-tag and without secretion signal

<400> 8
Met Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr 
1               5                   10                  15      
Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser 
            20                  25                  30          
Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr 
        35                  40                  45              
Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly 
    50                  55                  60                  
Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala 
65                  70                  75                  80  
Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly 
                85                  90                  95      
Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe 
            100                 105                 110         
Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val 
        115                 120                 125             
Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu 
    130                 135                 140                 
Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser 
145                 150                 155                 160 
Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln 
                165                 170                 175     
Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg 
            180                 185                 190         
Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys 
        195                 200                 205             
Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220                 
Gly Gly Gly Gly Ser Asp Ile Glu Lys Glu Ile Leu Asp Leu Ala Ala 
225                 230                 235                 240 
Ala Thr Glu Arg Leu Asn Leu Thr Asp Ala Leu Asn Ser Asn Pro Ala 
                245                 250                 255     
Gly Asn Leu Tyr Asp Trp Arg Ser Ser Asn Ser Tyr Pro Trp Thr Gln 
            260                 265                 270         
Lys Leu Asn Leu His Leu Thr Ile Thr Ala Thr Gly Gln Lys Tyr Arg 
        275                 280                 285             
Ile Leu Ala Ser Lys Ile Val Asp Phe Asn Ile Tyr Ser Asn Asn Phe 
    290                 295                 300                 
Asn Asn Leu Val Lys Leu Glu Gln Ser Leu Gly Asp Gly Val Lys Asp 
305                 310                 315                 320 
His Tyr Val Asp Ile Ser Leu Asp Ala Gly Gln Tyr Val Leu Val Met 
                325                 330                 335     
Lys Ala Asn Ser Ser Tyr Ser Gly Asn Tyr Pro Tyr Ser Ile Leu Phe 
            340                 345                 350         
Gln Lys Phe 
        355 

<210> 9
<211> 1294
<212> PRT
<213> Artificial Sequence


<220> 
<223> Stabilized Spike trimer with C-terminal His/Strep-tag including
      trimerization domain

<400> 9
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      
His Ala Ala Arg Pro Met Val Asn Leu Thr Thr Arg Thr Gln Leu Pro 
            20                  25                  30          
Pro Ala Tyr Thr Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys 
        35                  40                  45              
Val Phe Arg Ser Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro 
    50                  55                  60                  
Phe Phe Ser Asn Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr 
65                  70                  75                  80  
Asn Gly Thr Lys Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly 
                85                  90                  95      
Val Tyr Phe Ala Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile 
            100                 105                 110         
Phe Gly Thr Thr Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn 
        115                 120                 125             
Asn Ala Thr Asn Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn 
    130                 135                 140                 
Asp Pro Phe Leu Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met 
145                 150                 155                 160 
Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu 
                165                 170                 175     
Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn 
            180                 185                 190         
Phe Lys Asn Leu Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe 
        195                 200                 205             
Lys Ile Tyr Ser Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro 
    210                 215                 220                 
Gln Gly Phe Ser Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile 
225                 230                 235                 240 
Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu 
                245                 250                 255     
Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr 
            260                 265                 270         
Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu 
        275                 280                 285             
Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser 
    290                 295                 300                 
Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr 
305                 310                 315                 320 
Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe 
                325                 330                 335     
Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr 
            340                 345                 350         
Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys 
        355                 360                 365             
Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe 
    370                 375                 380                 
Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr 
385                 390                 395                 400 
Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln 
                405                 410                 415     
Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu 
            420                 425                 430         
Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu 
        435                 440                 445             
Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg 
    450                 455                 460                 
Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr 
465                 470                 475                 480 
Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr 
                485                 490                 495     
Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr 
            500                 505                 510         
Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro 
        515                 520                 525             
Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys 
    530                 535                 540                 
Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr 
545                 550                 555                 560 
Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile 
                565                 570                 575     
Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu 
            580                 585                 590         
Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly 
        595                 600                 605             
Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys 
    610                 615                 620                 
Thr Glu Val Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp 
625                 630                 635                 640 
Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys 
                645                 650                 655     
Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro 
            660                 665                 670         
Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro 
        675                 680                 685             
Gly Ala Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu 
    690                 695                 700                 
Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro 
705                 710                 715                 720 
Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met 
                725                 730                 735     
Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr 
            740                 745                 750         
Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu 
        755                 760                 765             
Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln 
    770                 775                 780                 
Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys 
785                 790                 795                 800 
Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys 
                805                 810                 815     
Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr 
            820                 825                 830         
Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp 
        835                 840                 845             
Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr 
    850                 855                 860                 
Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser 
865                 870                 875                 880 
Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly 
                885                 890                 895     
Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn 
            900                 905                 910         
Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile 
        915                 920                 925             
Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser 
    930                 935                 940                 
Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn 
945                 950                 955                 960 
Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly 
                965                 970                 975     
Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro 
            980                 985                 990         
Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser 
        995                 1000                1005            
Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg 
    1010                1015                1020                
Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly 
1025                1030                1035                1040
Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser 
                1045                1050                1055    
Phe Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr 
            1060                1065                1070        
Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His 
        1075                1080                1085            
Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly 
    1090                1095                1100                
Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile 
1105                1110                1115                1120
Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly 
                1125                1130                1135    
Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser 
            1140                1145                1150        
Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp 
        1155                1160                1165            
Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile 
    1170                1175                1180                
Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu 
1185                1190                1195                1200
Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys 
                1205                1210                1215    
Gly Ser Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val 
            1220                1225                1230        
Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Gly Glu Asn 
        1235                1240                1245            
Leu Tyr Phe Gln Gly Gly Ser Ala Trp Ser His Pro Gln Phe Glu Lys 
    1250                1255                1260                
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser Ala Trp Ser His Pro 
1265                1270                1275                1280
Gln Phe Glu Lys Gly Ser His His His His His His His His 
                1285                1290                

<210> 10
<211> 1224
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> Stabilized Spike trimer including trimerization domain

<400> 10
Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser 
1               5                   10                  15      
Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val 
            20                  25                  30          
Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr 
        35                  40                  45              
Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe 
    50                  55                  60                  
Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr 
65                  70                  75                  80  
Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp 
                85                  90                  95      
Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val 
            100                 105                 110         
Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val 
        115                 120                 125             
Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val 
    130                 135                 140                 
Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe 
145                 150                 155                 160 
Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu 
                165                 170                 175     
Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His 
            180                 185                 190         
Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu 
        195                 200                 205             
Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln 
    210                 215                 220                 
Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
225                 230                 235                 240 
Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln 
                245                 250                 255     
Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp 
            260                 265                 270         
Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
        275                 280                 285             
Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
    290                 295                 300                 
Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
305                 310                 315                 320 
Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
                325                 330                 335     
Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
            340                 345                 350         
Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
        355                 360                 365             
Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
    370                 375                 380                 
Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
385                 390                 395                 400 
Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Met Thr 
                405                 410                 415     
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
            420                 425                 430         
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
        435                 440                 445             
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
    450                 455                 460                 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
465                 470                 475                 480 
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
        515                 520                 525             
Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
    530                 535                 540                 
Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
545                 550                 555                 560 
Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
                565                 570                 575     
Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
            580                 585                 590         
Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
        595                 600                 605             
Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
    610                 615                 620                 
Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 
His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     
Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ala Ser Val Ala 
            660                 665                 670         
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
        675                 680                 685             
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
    690                 695                 700                 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
705                 710                 715                 720 
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
                725                 730                 735     
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
            740                 745                 750         
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
        755                 760                 765             
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
    770                 775                 780                 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
785                 790                 795                 800 
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
                805                 810                 815     
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
            820                 825                 830         
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
        835                 840                 845             
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
    850                 855                 860                 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
865                 870                 875                 880 
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
                885                 890                 895     
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
            900                 905                 910         
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
        915                 920                 925             
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
    930                 935                 940                 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
945                 950                 955                 960 
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
                965                 970                 975     
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
            980                 985                 990         
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
        995                 1000                1005            
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
    1010                1015                1020                
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
1025                1030                1035                1040
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
                1045                1050                1055    
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
            1060                1065                1070        
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
        1075                1080                1085            
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
    1090                1095                1100                
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
1105                1110                1115                1120
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
                1125                1130                1135    
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
            1140                1145                1150        
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
        1155                1160                1165            
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
    1170                1175                1180                
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Gly Ser Gly Tyr Ile 
1185                1190                1195                1200
Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu 
                1205                1210                1215    
Trp Val Leu Leu Ser Thr Phe Leu 
            1220                

<210> 11
<211> 1396
<212> PRT
<213> Artificial Sequence


<220> 
<223> Stabilized Spike-C-CPE trimer with N-terminal His-tag, C-terminal
      trimerization domain and secretion signal

<400> 11
Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      
Val Gln Ala Met Gly His His His His His His His His His Glu Asn 
            20                  25                  30          
Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Met Val Asn Leu Thr Thr 
        35                  40                  45              
Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe Thr Arg Gly Val 
    50                  55                  60                  
Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu His Ser Thr Gln 
65                  70                  75                  80  
Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp Phe His Ala Ile 
                85                  90                  95      
His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro Val Leu 
            100                 105                 110         
Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser Asn Ile 
        115                 120                 125             
Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr Gln Ser 
    130                 135                 140                 
Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val Cys Glu 
145                 150                 155                 160 
Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr Tyr His Lys Asn 
                165                 170                 175     
Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn 
            180                 185                 190         
Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu 
        195                 200                 205             
Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys Asn 
    210                 215                 220                 
Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn Leu 
225                 230                 235                 240 
Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val Asp 
                245                 250                 255     
Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala Leu 
            260                 265                 270         
His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala 
        275                 280                 285             
Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu 
    290                 295                 300                 
Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala 
305                 310                 315                 320 
Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr Val 
                325                 330                 335     
Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Glu 
            340                 345                 350         
Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu 
        355                 360                 365             
Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys 
    370                 375                 380                 
Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala 
385                 390                 395                 400 
Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn 
                405                 410                 415     
Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly 
            420                 425                 430         
Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
        435                 440                 445             
Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp 
    450                 455                 460                 
Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu 
465                 470                 475                 480 
Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile 
                485                 490                 495     
Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu 
            500                 505                 510         
Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr 
        515                 520                 525             
Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu 
    530                 535                 540                 
Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn 
545                 550                 555                 560 
Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly 
                565                 570                 575     
Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln 
            580                 585                 590         
Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln 
        595                 600                 605             
Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser 
    610                 615                 620                 
Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr 
625                 630                 635                 640 
Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln 
                645                 650                 655     
Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln 
            660                 665                 670         
Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr 
        675                 680                 685             
Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr 
    690                 695                 700                 
Gln Thr Asn Ser Pro Gly Ala Ser Val Ala Ser Gln Ser Ile Ile Ala 
705                 710                 715                 720 
Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn 
                725                 730                 735     
Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile 
            740                 745                 750         
Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile 
        755                 760                 765             
Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser 
    770                 775                 780                 
Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln 
785                 790                 795                 800 
Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys 
                805                 810                 815     
Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu 
            820                 825                 830         
Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu 
        835                 840                 845             
Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly 
    850                 855                 860                 
Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys 
865                 870                 875                 880 
Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile 
                885                 890                 895     
Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp 
            900                 905                 910         
Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met 
        915                 920                 925             
Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu 
    930                 935                 940                 
Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile 
945                 950                 955                 960 
Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp 
                965                 970                 975     
Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu 
            980                 985                 990         
Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser 
        995                 1000                1005            
Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr 
    1010                1015                1020                
Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg 
1025                1030                1035                1040
Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser 
                1045                1050                1055    
Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly 
            1060                1065                1070        
Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe 
        1075                1080                1085            
Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala 
    1090                1095                1100                
Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val 
1105                1110                1115                1120
Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr 
                1125                1130                1135    
Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys 
            1140                1145                1150        
Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln 
        1155                1160                1165            
Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn 
    1170                1175                1180                
His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala 
1185                1190                1195                1200
Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala 
                1205                1210                1215    
Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr 
            1220                1225                1230        
Glu Gln Tyr Ile Lys Gly Ser Gly Tyr Ile Pro Glu Ala Pro Arg Asp 
        1235                1240                1245            
Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu Trp Val Leu Leu Ser Thr 
    1250                1255                1260                
Phe Leu Gly Gly Gly Ser Asp Ile Glu Lys Glu Ile Leu Asp Leu Ala 
1265                1270                1275                1280
Ala Ala Thr Glu Arg Leu Asn Leu Thr Asp Ala Leu Asn Ser Asn Pro 
                1285                1290                1295    
Ala Gly Asn Leu Tyr Asp Trp Arg Ser Ser Asn Ser Tyr Pro Trp Thr 
            1300                1305                1310        
Gln Lys Leu Asn Leu His Leu Thr Ile Thr Ala Thr Gly Gln Lys Tyr 
        1315                1320                1325            
Arg Ile Leu Ala Ser Lys Ile Val Asp Phe Asn Ile Tyr Ser Asn Asn 
    1330                1335                1340                
Phe Asn Asn Leu Val Lys Leu Glu Gln Ser Leu Gly Asp Gly Val Lys 
1345                1350                1355                1360
Asp His Tyr Val Asp Ile Ser Leu Asp Ala Gly Gln Tyr Val Leu Val 
                1365                1370                1375    
Met Lys Ala Asn Ser Ser Tyr Ser Gly Asn Tyr Pro Tyr Ser Ile Leu 
            1380                1385                1390        
Phe Gln Lys Phe 
        1395    

<210> 12
<211> 1354
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> Stabilized Spike-C-CPE trimer with trimerization domain

<400> 12
Met Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
1               5                   10                  15      
Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser 
            20                  25                  30          
Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val 
        35                  40                  45              
Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg 
    50                  55                  60                  
Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser 
65                  70                  75                  80  
Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu 
                85                  90                  95      
Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val 
            100                 105                 110         
Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly 
        115                 120                 125             
Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg 
    130                 135                 140                 
Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro 
145                 150                 155                 160 
Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg 
                165                 170                 175     
Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys 
            180                 185                 190         
His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala 
        195                 200                 205             
Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe 
    210                 215                 220                 
Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser 
225                 230                 235                 240 
Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
                245                 250                 255     
Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr 
            260                 265                 270         
Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr 
        275                 280                 285             
Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe 
    290                 295                 300                 
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
305                 310                 315                 320 
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
                325                 330                 335     
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
            340                 345                 350         
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
        355                 360                 365             
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
    370                 375                 380                 
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
385                 390                 395                 400 
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
                405                 410                 415     
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
            420                 425                 430         
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
        435                 440                 445             
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
    450                 455                 460                 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
465                 470                 475                 480 
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
        515                 520                 525             
Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
    530                 535                 540                 
Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
545                 550                 555                 560 
Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
                565                 570                 575     
Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
            580                 585                 590         
Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
        595                 600                 605             
Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
    610                 615                 620                 
Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 
His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     
Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ala Ser Val Ala 
            660                 665                 670         
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
        675                 680                 685             
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
    690                 695                 700                 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
705                 710                 715                 720 
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
                725                 730                 735     
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
            740                 745                 750         
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
        755                 760                 765             
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
    770                 775                 780                 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
785                 790                 795                 800 
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
                805                 810                 815     
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
            820                 825                 830         
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
        835                 840                 845             
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
    850                 855                 860                 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
865                 870                 875                 880 
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
                885                 890                 895     
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
            900                 905                 910         
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
        915                 920                 925             
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
    930                 935                 940                 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
945                 950                 955                 960 
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
                965                 970                 975     
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
            980                 985                 990         
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
        995                 1000                1005            
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
    1010                1015                1020                
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
1025                1030                1035                1040
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
                1045                1050                1055    
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
            1060                1065                1070        
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
        1075                1080                1085            
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
    1090                1095                1100                
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
1105                1110                1115                1120
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
                1125                1130                1135    
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
            1140                1145                1150        
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
        1155                1160                1165            
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
    1170                1175                1180                
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Gly Ser Gly Tyr Ile 
1185                1190                1195                1200
Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys Asp Gly Glu 
                1205                1210                1215    
Trp Val Leu Leu Ser Thr Phe Leu Gly Gly Gly Ser Asp Ile Glu Lys 
            1220                1225                1230        
Glu Ile Leu Asp Leu Ala Ala Ala Thr Glu Arg Leu Asn Leu Thr Asp 
        1235                1240                1245            
Ala Leu Asn Ser Asn Pro Ala Gly Asn Leu Tyr Asp Trp Arg Ser Ser 
    1250                1255                1260                
Asn Ser Tyr Pro Trp Thr Gln Lys Leu Asn Leu His Leu Thr Ile Thr 
1265                1270                1275                1280
Ala Thr Gly Gln Lys Tyr Arg Ile Leu Ala Ser Lys Ile Val Asp Phe 
                1285                1290                1295    
Asn Ile Tyr Ser Asn Asn Phe Asn Asn Leu Val Lys Leu Glu Gln Ser 
            1300                1305                1310        
Leu Gly Asp Gly Val Lys Asp His Tyr Val Asp Ile Ser Leu Asp Ala 
        1315                1320                1325            
Gly Gln Tyr Val Leu Val Met Lys Ala Asn Ser Ser Tyr Ser Gly Asn 
    1330                1335                1340                
Tyr Pro Tyr Ser Ile Leu Phe Gln Lys Phe 
1345                1350                

<210> 13
<211> 1367
<212> PRT
<213> Artificial Sequence


<220> 
<223> Stabilized Spike-C-CPE monomer with N-terminal His-tag and
      secretion signal

<400> 13
Met Asn Phe Gly Leu Arg Leu Ile Phe Leu Val Leu Thr Leu Lys Gly 
1               5                   10                  15      
Val Gln Ala Met Gly His His His His His His His His His Glu Asn 
            20                  25                  30          
Leu Tyr Phe Gln Gly Gly Gly Gly Ser Gly Met Val Asn Leu Thr Thr 
        35                  40                  45              
Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe Thr Arg Gly Val 
    50                  55                  60                  
Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu His Ser Thr Gln 
65                  70                  75                  80  
Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp Phe His Ala Ile 
                85                  90                  95      
His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro Val Leu 
            100                 105                 110         
Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser Asn Ile 
        115                 120                 125             
Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr Gln Ser 
    130                 135                 140                 
Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val Cys Glu 
145                 150                 155                 160 
Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr Tyr His Lys Asn 
                165                 170                 175     
Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala Asn 
            180                 185                 190         
Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu Glu 
        195                 200                 205             
Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys Asn 
    210                 215                 220                 
Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn Leu 
225                 230                 235                 240 
Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val Asp 
                245                 250                 255     
Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala Leu 
            260                 265                 270         
His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr Ala 
        275                 280                 285             
Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe Leu 
    290                 295                 300                 
Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys Ala 
305                 310                 315                 320 
Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr Val 
                325                 330                 335     
Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr Glu 
            340                 345                 350         
Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu 
        355                 360                 365             
Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys 
    370                 375                 380                 
Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala 
385                 390                 395                 400 
Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn 
                405                 410                 415     
Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly 
            420                 425                 430         
Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp 
        435                 440                 445             
Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp 
    450                 455                 460                 
Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu 
465                 470                 475                 480 
Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile 
                485                 490                 495     
Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu 
            500                 505                 510         
Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr 
        515                 520                 525             
Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu 
    530                 535                 540                 
Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn 
545                 550                 555                 560 
Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr Gly 
                565                 570                 575     
Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln Gln 
            580                 585                 590         
Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro Gln 
        595                 600                 605             
Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val Ser 
    610                 615                 620                 
Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu Tyr 
625                 630                 635                 640 
Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp Gln 
                645                 650                 655     
Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe Gln 
            660                 665                 670         
Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser Tyr 
        675                 680                 685             
Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln Thr 
    690                 695                 700                 
Gln Thr Asn Ser Pro Gly Ala Ser Val Ala Ser Gln Ser Ile Ile Ala 
705                 710                 715                 720 
Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn 
                725                 730                 735     
Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr Thr Glu Ile 
            740                 745                 750         
Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr Met Tyr Ile 
        755                 760                 765             
Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser 
    770                 775                 780                 
Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala Val Glu Gln 
785                 790                 795                 800 
Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln Ile Tyr Lys 
                805                 810                 815     
Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu 
            820                 825                 830         
Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu 
        835                 840                 845             
Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly 
    850                 855                 860                 
Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys 
865                 870                 875                 880 
Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp Glu Met Ile 
                885                 890                 895     
Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp 
            900                 905                 910         
Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala Met Gln Met 
        915                 920                 925             
Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val Leu Tyr Glu 
    930                 935                 940                 
Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile 
945                 950                 955                 960 
Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp 
                965                 970                 975     
Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val Lys Gln Leu 
            980                 985                 990         
Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp Ile Leu Ser 
        995                 1000                1005            
Arg Leu Asp Pro Pro Glu Ala Glu Val Gln Ile Asp Arg Leu Ile Thr 
    1010                1015                1020                
Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg 
1025                1030                1035                1040
Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr Lys Met Ser 
                1045                1050                1055    
Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys Gly Lys Gly 
            1060                1065                1070        
Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly Val Val Phe 
        1075                1080                1085            
Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala 
    1090                1095                1100                
Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro Arg Glu Gly Val 
1105                1110                1115                1120
Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg Asn Phe Tyr 
                1125                1130                1135    
Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser Gly Asn Cys 
            1140                1145                1150        
Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp Pro Leu Gln 
        1155                1160                1165            
Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn 
    1170                1175                1180                
His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala 
1185                1190                1195                1200
Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn Glu Val Ala 
                1205                1210                1215    
Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr 
            1220                1225                1230        
Glu Gln Tyr Ile Lys Gly Gly Gly Ser Asp Ile Glu Lys Glu Ile Leu 
        1235                1240                1245            
Asp Leu Ala Ala Ala Thr Glu Arg Leu Asn Leu Thr Asp Ala Leu Asn 
    1250                1255                1260                
Ser Asn Pro Ala Gly Asn Leu Tyr Asp Trp Arg Ser Ser Asn Ser Tyr 
1265                1270                1275                1280
Pro Trp Thr Gln Lys Leu Asn Leu His Leu Thr Ile Thr Ala Thr Gly 
                1285                1290                1295    
Gln Lys Tyr Arg Ile Leu Ala Ser Lys Ile Val Asp Phe Asn Ile Tyr 
            1300                1305                1310        
Ser Asn Asn Phe Asn Asn Leu Val Lys Leu Glu Gln Ser Leu Gly Asp 
        1315                1320                1325            
Gly Val Lys Asp His Tyr Val Asp Ile Ser Leu Asp Ala Gly Gln Tyr 
    1330                1335                1340                
Val Leu Val Met Lys Ala Asn Ser Ser Tyr Ser Gly Asn Tyr Pro Tyr 
1345                1350                1355                1360
Ser Ile Leu Phe Gln Lys Phe 
                1365        

<210> 14
<211> 1325
<212> PRT
<213> Artificial Sequence


<220> 
<221> SITE
<222> 1..1
<223> optionally present

<220> 
<223> Stabilized Spike-C-CPE monomer

<400> 14
Met Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn 
1               5                   10                  15      
Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser 
            20                  25                  30          
Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val 
        35                  40                  45              
Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg 
    50                  55                  60                  
Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser 
65                  70                  75                  80  
Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu 
                85                  90                  95      
Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val 
            100                 105                 110         
Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly 
        115                 120                 125             
Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg 
    130                 135                 140                 
Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro 
145                 150                 155                 160 
Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg 
                165                 170                 175     
Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys 
            180                 185                 190         
His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala 
        195                 200                 205             
Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe 
    210                 215                 220                 
Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser 
225                 230                 235                 240 
Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu 
                245                 250                 255     
Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr 
            260                 265                 270         
Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr 
        275                 280                 285             
Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe 
    290                 295                 300                 
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
305                 310                 315                 320 
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
                325                 330                 335     
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
            340                 345                 350         
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
        355                 360                 365             
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
    370                 375                 380                 
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
385                 390                 395                 400 
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
                405                 410                 415     
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
            420                 425                 430         
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
        435                 440                 445             
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
    450                 455                 460                 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
465                 470                 475                 480 
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
                485                 490                 495     
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
            500                 505                 510         
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
        515                 520                 525             
Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys 
    530                 535                 540                 
Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp 
545                 550                 555                 560 
Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys 
                565                 570                 575     
Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn 
            580                 585                 590         
Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val 
        595                 600                 605             
Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr 
    610                 615                 620                 
Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu 
625                 630                 635                 640 
His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile 
                645                 650                 655     
Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ala Ser Val Ala 
            660                 665                 670         
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
        675                 680                 685             
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
    690                 695                 700                 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
705                 710                 715                 720 
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
                725                 730                 735     
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
            740                 745                 750         
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
        755                 760                 765             
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
    770                 775                 780                 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
785                 790                 795                 800 
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
                805                 810                 815     
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
            820                 825                 830         
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
        835                 840                 845             
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
    850                 855                 860                 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
865                 870                 875                 880 
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
                885                 890                 895     
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
            900                 905                 910         
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
        915                 920                 925             
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
    930                 935                 940                 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
945                 950                 955                 960 
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
                965                 970                 975     
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
            980                 985                 990         
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
        995                 1000                1005            
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
    1010                1015                1020                
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
1025                1030                1035                1040
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
                1045                1050                1055    
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
            1060                1065                1070        
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
        1075                1080                1085            
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
    1090                1095                1100                
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
1105                1110                1115                1120
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
                1125                1130                1135    
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
            1140                1145                1150        
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
        1155                1160                1165            
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
    1170                1175                1180                
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Gly Gly Gly Ser Asp 
1185                1190                1195                1200
Ile Glu Lys Glu Ile Leu Asp Leu Ala Ala Ala Thr Glu Arg Leu Asn 
                1205                1210                1215    
Leu Thr Asp Ala Leu Asn Ser Asn Pro Ala Gly Asn Leu Tyr Asp Trp 
            1220                1225                1230        
Arg Ser Ser Asn Ser Tyr Pro Trp Thr Gln Lys Leu Asn Leu His Leu 
        1235                1240                1245            
Thr Ile Thr Ala Thr Gly Gln Lys Tyr Arg Ile Leu Ala Ser Lys Ile 
    1250                1255                1260                
Val Asp Phe Asn Ile Tyr Ser Asn Asn Phe Asn Asn Leu Val Lys Leu 
1265                1270                1275                1280
Glu Gln Ser Leu Gly Asp Gly Val Lys Asp His Tyr Val Asp Ile Ser 
                1285                1290                1295    
Leu Asp Ala Gly Gln Tyr Val Leu Val Met Lys Ala Asn Ser Ser Tyr 
            1300                1305                1310        
Ser Gly Asn Tyr Pro Tyr Ser Ile Leu Phe Gln Lys Phe 
        1315                1320                1325

