                       SEQUENCE LISTING

<110>  Precirix N.V.

<120>  Antibody fragment against FOLR1

<130>  P6091371PCT

<150> 20185584.8
<151> 2020-07-13

<160>  86

<210>  1
<211>  257
<212>  PRT
<213>  Human

<220>
<223>  Human FOLR1

<400>  1
Met Ala Gln Arg Met Thr Thr Gln Leu Leu Leu Leu Leu Val Trp 
1               5                   10                  15  

Val Ala Val Val Gly Glu Ala Gln Thr Arg Ile Ala Trp Ala Arg 
                20                  25                  30  

Thr Glu Leu Leu Asn Val Cys Met Asn Ala Lys His His Lys Glu 
                35                  40                  45  

Lys Pro Gly Pro Glu Asp Lys Leu His Glu Gln Cys Arg Pro Trp 
                50                  55                  60  

Arg Lys Asn Ala Cys Cys Ser Thr Asn Thr Ser Gln Glu Ala His 
                65                  70                  75  

Lys Asp Val Ser Tyr Leu Tyr Arg Phe Asn Trp Asn His Cys Gly 
                80                  85                  90  

Glu Met Ala Pro Ala Cys Lys Arg His Phe Ile Gln Asp Thr Cys 
                95                  100                 105 

Leu Tyr Glu Cys Ser Pro Asn Leu Gly Pro Trp Ile Gln Gln Val 
                110                 115                 120 

Asp Gln Ser Trp Arg Lys Glu Arg Val Leu Asn Val Pro Leu Cys 
                125                 130                 135 

Lys Glu Asp Cys Glu Gln Trp Trp Glu Asp Cys Arg Thr Ser Tyr 
                140                 145                 150 

Thr Cys Lys Ser Asn Trp His Lys Gly Trp Asn Trp Thr Ser Gly 
                155                 160                 165 

Phe Asn Lys Cys Ala Val Gly Ala Ala Cys Gln Pro Phe His Phe 
                170                 175                 180 

Tyr Phe Pro Thr Pro Thr Val Leu Cys Asn Glu Ile Trp Thr His 
                185                 190                 195 

Ser Tyr Lys Val Ser Asn Tyr Ser Arg Gly Ser Gly Arg Cys Ile 
                200                 205                 210 

Gln Met Trp Phe Asp Pro Ala Gln Gly Asn Pro Asn Glu Glu Val 
                215                 220                 225 

Ala Arg Phe Tyr Ala Ala Ala Met Ser Gly Ala Gly Pro Trp Ala 
                230                 235                 240 

Ala Trp Pro Phe Leu Leu Ser Leu Ala Leu Met Leu Leu Trp Leu 
                245                 250                 255 

Leu Ser 
        

<210>  2
<211>  78
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IMGT: part of A1: N37-W118 = longest region of A1 interacting with human FOLR1

<400>  2
Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu 
1               5                   10                  15  

Leu Ala Ala Ile Ile Thr Ser Asp Gly Asn Thr Asn Tyr Pro Asp 
                20                  25                  30  

Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                35                  40                  45  

Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala 
                50                  55                  60  

Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe Tyr 
                65                  70                  75  

Asp Tyr Trp 
            

<210>  3
<211>  76
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IMGT: part of A2: D38-W118 = longest region of A2 interacting with human FOLR1

<400>  3
Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg Glu Leu 
1               5                   10                  15  

Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro Asp Ser 
                20                  25                  30  

Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr 
                35                  40                  45  

Val Tyr Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
                50                  55                  60  

Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser Asp Tyr 
                65                  70                  75  

Trp 
    

<210>  4
<211>  30
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IMGT: 1st of 2 discrete regions of A1 interacting with human FOLR1 = N37-D69

<400>  4
Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu 
1               5                   10                  15  

Leu Ala Ala Ile Ile Thr Ser Asp Gly Asn Thr Asn Tyr Pro Asp 
                20                  25                  30  




<210>  5
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IMGT: 2nd of 2 discrete regions of A1 interacting with human FOLR1 = N105-W118

<400>  5
Asn Thr Phe Gly Arg Ser Val Ala Gly Phe Tyr Asp Tyr Trp 
1               5                   10                  

<210>  6
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IMGT: 1ST of 2 discrete regions of A2 interacting with human FOLR1 = D38-D69

<400>  6
Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg Glu Leu 
1               5                   10                  15  

Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro Asp 
                20                  25                  

<210>  7
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  IMGT: 2nd  of 2 discrete regions of A2 interacting with human FOLR1 = N105-W118

<400>  7
Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser Asp Tyr Trp 
1               5                   10              

<210>  8
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Full length sequence of A1

<400>  8
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Asp Gly Asn Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115                 

<210>  9
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Full length sequence of A2

<400>  9
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Met Lys Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115             

<210>  10
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of A1

<400>  10
Glu Ser Ile Phe Ser Arg Asn His 
1               5               

<210>  11
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of A1

<400>  11
Ile Thr Ser Asp Gly Asn Thr 
1               5           

<210>  12
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of A1

<400>  12
Asn Thr Phe Gly Arg Ser Val Ala Gly Phe Tyr Asp Tyr 
1               5                   10              

<210>  13
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of A1

<400>  13
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                20                  25  

<210>  14
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of A1

<400>  14
Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Ala 
1               5                   10                  15  

Ala Ile 
        

<210>  15
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of A1

<400>  15
Asn Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  16
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of A1

<400>  16
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  17
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of A2

<400>  17
Glu Met Lys Phe Ser Asn Tyr Asp 
1               5               

<210>  18
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of A2

<400>  18
Ile Thr Ser Gly Gly Ser Thr 
1               5           

<210>  19
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of A2

<400>  19
Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser Asp Tyr 
1               5                   10          

<210>  20
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of A2

<400>  20
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp 
                20                  25  

<210>  21
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of A2

<400>  21
Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg Glu Leu Ala 
1               5                   10                  15  

Ala Ile 
        

<210>  22
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of A2

<400>  22
Asn Tyr Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Thr Val Tyr Leu Glu Met Asn Ser Leu Lys Pro 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  23
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of A2

<400>  23
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  24
<211>  121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Full length sequence of A3

<400>  24
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser Gly Asn Met Phe Ser 
                20                  25                  30  

Met Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg 
                35                  40                  45  

Asp Trp Val Ala Val Ile Thr Lys Asp Gly Ser Thr Asn Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Val Gln Asp Gly Ser Gly Trp Ile Arg 
                95                  100                 105 

Ala Trp Gly Trp Asp Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
                110                 115                 120 

Ser 
    

<210>  25
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of A3

<400>  25
Gly Asn Met Phe Ser Met Asn Leu 
1               5               

<210>  26
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of A3

<400>  26
Ile Thr Lys Asp Gly Ser Thr 
1               5           

<210>  27
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of A3

<400>  27
Asn Val Gln Asp Gly Ser Gly Trp Ile Arg Ala Trp Gly Trp Asp 
1               5                   10                  15  




<210>  28
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of A3

<400>  28
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser 
                20                  25  

<210>  29
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of A3

<400>  29
Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg Asp Trp Val 
1               5                   10                  15  

Ala Val 
        

<210>  30
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of A3

<400>  30
Asn Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  31
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of A3

<400>  31
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  32
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Full length sequence of A4

<400>  32
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Arg Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ala Phe Ser 
                20                  25                  30  

Ser Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu 
                35                  40                  45  

Arg Glu Phe Val Ala Asp Ile Asn Trp Ser Glu Gly Ser Pro Met 
                50                  55                  60  

Tyr Ile Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn 
                65                  70                  75  

Ala Arg Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu 
                80                  85                  90  

Asp Thr Ala Val Tyr Tyr Cys Ala Ala Arg Arg Phe Gly Gln Gly 
                95                  100                 105 

Tyr Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115                 

<210>  33
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of A4

<400>  33
Gly Arg Ala Phe Ser Ser Thr Tyr Ala 
1               5                   

<210>  34
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of A4

<400>  34
Ile Asn Trp Ser Glu Gly Ser Pro 
1               5               

<210>  35
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of A4

<400>  35
Ala Ala Arg Arg Phe Gly Gln Gly Tyr Glu Tyr 
1               5                   10      

<210>  36
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of A4

<400>  36
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Arg Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
                20                  25  

<210>  37
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of A4

<400>  37
Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
1               5                   10                  15  

Ala Asp 
        

<210>  38
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of A4

<400>  38
Met Tyr Ile Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Arg Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  39
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of A4

<400>  39
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  40
<211>  121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Full length sequence of A5

<400>  40
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser Gly Asn Ile Phe Ser 
                20                  25                  30  

Met Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg 
                35                  40                  45  

Glu Trp Val Ala Leu Ile Thr Lys Asp Gly Ser Thr Asn Tyr Glu 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Gln Asp Gly Ser Gly Trp Ile Arg 
                95                  100                 105 

Ala Trp Gly Trp Asp Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
                110                 115                 120 

Ser 
    

<210>  41
<211>  8
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR1 of A5

<400>  41
Gly Asn Ile Phe Ser Met Asn Leu 
1               5               

<210>  42
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR2 of A5

<400>  42
Ile Thr Lys Asp Gly Ser Thr 
1               5           

<210>  43
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  CDR3 of A5

<400>  43
Asn Ala Gln Asp Gly Ser Gly Trp Ile Arg Ala Trp Gly Trp Asp 
1               5                   10                  15  




<210>  44
<211>  25
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR1 of A5

<400>  44
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser 
                20                  25  

<210>  45
<211>  17
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR2 of A5

<400>  45
Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg Glu Trp Val 
1               5                   10                  15  

Ala Leu 
        

<210>  46
<211>  38
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR3 of A5

<400>  46
Asn Tyr Glu Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
1               5                   10                  15  

Asn Ala Lys Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro 
                20                  25                  30  

Glu Asp Thr Ala Val Tyr Tyr Cys 
                35              

<210>  47
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  FR4 of A5

<400>  47
Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10      

<210>  48
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tagged variant of A1

<400>  48
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Asp Gly Asn Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly 
                110                 115                 120 

Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
                125                 130                 

<210>  49
<211>  133
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tagged variant of A2

<400>  49
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Met Lys Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg 
                110                 115                 120 

Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
                125                 130             

<210>  50
<211>  136
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tagged variant of A3

<400>  50
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser Gly Asn Met Phe Ser 
                20                  25                  30  

Met Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg 
                35                  40                  45  

Asp Trp Val Ala Val Ile Thr Lys Asp Gly Ser Thr Asn Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Val Gln Asp Gly Ser Gly Trp Ile Arg 
                95                  100                 105 

Ala Trp Gly Trp Asp Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
                110                 115                 120 

Ser Gly Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg 
                125                 130                 135 

Ala 
    

<210>  51
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tagged variant of A4

<400>  51
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Ala Arg Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ala Phe Ser 
                20                  25                  30  

Ser Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu 
                35                  40                  45  

Arg Glu Phe Val Ala Asp Ile Asn Trp Ser Glu Gly Ser Pro Met 
                50                  55                  60  

Tyr Ile Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn 
                65                  70                  75  

Ala Arg Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu 
                80                  85                  90  

Asp Thr Ala Val Tyr Tyr Cys Ala Ala Arg Arg Phe Gly Gln Gly 
                95                  100                 105 

Tyr Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly 
                110                 115                 120 

Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
                125                 130                 

<210>  52
<211>  136
<212>  PRT
<213>  Artificial sequence

<220>
<223>  HA-tagged variant of A5

<400>  52
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser Gly Asn Ile Phe Ser 
                20                  25                  30  

Met Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg 
                35                  40                  45  

Glu Trp Val Ala Leu Ile Thr Lys Asp Gly Ser Thr Asn Tyr Glu 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Gln Asp Gly Ser Gly Trp Ile Arg 
                95                  100                 105 

Ala Trp Gly Trp Asp Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
                110                 115                 120 

Ser Gly Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg 
                125                 130                 135 

Ala 
    

<210>  53
<211>  125
<212>  PRT
<213>  Artificial sequence

<220>
<223>  His6-tagged variant of A1

<400>  53
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Asp Gly Asn Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His 
                110                 115                 120 

His His His His His 
                125 

<210>  54
<211>  124
<212>  PRT
<213>  Artificial sequence

<220>
<223>  His6-tagged variant of A2

<400>  54
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Met Lys Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His 
                110                 115                 120 

His His His His 
                

<210>  55
<211>  127
<212>  PRT
<213>  Artificial sequence

<220>
<223>  His6-tagged variant of A3

<400>  55
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser Gly Asn Met Phe Ser 
                20                  25                  30  

Met Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg 
                35                  40                  45  

Asp Trp Val Ala Val Ile Thr Lys Asp Gly Ser Thr Asn Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Val Gln Asp Gly Ser Gly Trp Ile Arg 
                95                  100                 105 

Ala Trp Gly Trp Asp Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
                110                 115                 120 

Ser His His His His His His 
                125         

<210>  56
<211>  125
<212>  PRT
<213>  Artificial sequence

<220>
<223>  His6-tagged variant of A4

<400>  56
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Ala Arg Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Ala Phe Ser 
                20                  25                  30  

Ser Thr Tyr Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu 
                35                  40                  45  

Arg Glu Phe Val Ala Asp Ile Asn Trp Ser Glu Gly Ser Pro Met 
                50                  55                  60  

Tyr Ile Asp Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn 
                65                  70                  75  

Ala Arg Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu 
                80                  85                  90  

Asp Thr Ala Val Tyr Tyr Cys Ala Ala Arg Arg Phe Gly Gln Gly 
                95                  100                 105 

Tyr Glu Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His 
                110                 115                 120 

His His His His His 
                125 

<210>  57
<211>  127
<212>  PRT
<213>  Artificial sequence

<220>
<223>  His6-tagged variant of A5

<400>  57
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Gly Val Ser Gly Asn Ile Phe Ser 
                20                  25                  30  

Met Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg 
                35                  40                  45  

Glu Trp Val Ala Leu Ile Thr Lys Asp Gly Ser Thr Asn Tyr Glu 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Gln Asp Gly Ser Gly Trp Ile Arg 
                95                  100                 105 

Ala Trp Gly Trp Asp Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
                110                 115                 120 

Ser His His His His His His 
                125         

<210>  58
<211>  774
<212>  DNA
<213>  Human

<220>
<223>  Human FOLR1

<400>  58
atggctcagc ggatgacaac acagctgctg ctccttctag tgtgggtggc                 50
tgtagtaggg gaggctcaga caaggattgc atgggccagg actgagcttc                 100
tcaatgtctg catgaacgcc aagcaccaca aggaaaagcc aggccccgag                 150
gacaagttgc atgagcagtg tcgaccctgg aggaagaatg cctgctgttc                 200
taccaacacc agccaggaag cccataagga tgtttcctac ctatatagat                 250
tcaactggaa ccactgtgga gagatggcac ctgcctgcaa acggcatttc                 300
atccaggaca cctgcctcta cgagtgctcc cccaacttgg ggccctggat                 350
ccagcaggtg gatcagagct ggcgcaaaga gcgggtactg aacgtgcccc                 400
tgtgcaaaga ggactgtgag caatggtggg aagattgtcg cacctcctac                 450
acctgcaaga gcaactggca caagggctgg aactggactt cagggtttaa                 500
caagtgcgca gtgggagctg cctgccaacc tttccatttc tacttcccca                 550
cacccactgt tctgtgcaat gaaatctgga ctcactccta caaggtcagc                 600
aactacagcc gagggagtgg ccgctgcatc cagatgtggt tcgacccagc                 650
ccagggcaac cccaatgagg aggtggcgag gttctatgct gcagccatga                 700
gtggggctgg gccctgggca gcctggcctt tcctgcttag cctggcccta                 750
atgctgctgt ggctgctcag ctga                                             774

<210>  59
<211>  15927
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Targeting vector to knock-in human folr1 cDNA after the stop-codon in exon 4 of the mouse folr1 gene, by homologous recombination

<400>  59
cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc                 50
gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt                 100
gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg                 150
ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat                 200
tggagctcca ccgcccgggc tggttctttc cgcctcagaa gccatagagc                 250
ccaccgcatc cccagcatgc ctgctattgt cttcccaatc ctcccccttg                 300
ctgtcctgcc ccaccccacc ccccagaata gaatgacacc tactcagaca                 350
atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac                 400
cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg                 450
caactagaag gcacagtcga ggctgatcag cgagctctag gatctgcatt                 500
ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac                 550
aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata                 600
tttaccttag agctttaaat ctctgtaggt agtttgtcca attatgtcac                 650
accacagaag taaggttcct tcacaaagag atcgcctgac acgatttcct                 700
gcacaggctt gagccatata ctcatacatc gcatcttggc cacgttttcc                 750
acgggtttca aaattaatct caagttctac gcttaacgct ttcgcctgtt                 800
cccagttatt aatatattca acgctagaac tcccctcagc gaagggaagg                 850
ctgagcacta cacgcgaagc accatcaccg aaccttttga taaactcttc                 900
cgttccgact tgctccatca acggttcagt gagacttaaa cctaactctt                 950
tcttaatagt ttcggcatta tccactttta gtgcgagaac cttcgtcagt                 1000
cctggatacg tcactttgac cacgcctcca gcttttccag agagcgggtt                 1050
ttcattatct acagagtatc ccgcagcgtc gtatttattg tcggtactat                 1100
aaaacccttt ccaatcatcg tcataatttc cttgtgtacc agattttggc                 1150
ttttgtatac ctttttgaat ggaatctaca taaccaggtt tagtcccgtg                 1200
gtacgaagaa aagttttcca tcacaaaaga tttagaagaa tcaacaacat                 1250
catcaggatc catggcacgc gcttctacaa ggcgctggcc gaagaggtgc                 1300
gggagtttca cgccaccaag atctgcggca cgctgttgac gctgttaagc                 1350
gggtcgctgc agggtcgctc ggtgttcgag gccacacgcg tcaccttaat                 1400
atgcgaagtg gacctgggac cgcgccgccc cgactgcatc tgcgtgttcg                 1450
aattcgccaa tgacaagacg ctgggcgggg tttgctcgac attgggtgga                 1500
aacattccag gcctgggtgg agaggctttt tgcttcctct tgcaaaacca                 1550
cactgctcga cattgggtgg aaacattcca ggcctgggtg gagaggcttt                 1600
ttgcttcctc ttgaaaacca cactgctcga tttgttagca gcctcgaatc                 1650
aacccgggcg atcctaggcg atgagatcta gctgtcgcga agagtggcgc                 1700
gccagcctgg gcctacaaac aaacaaacaa acaaattcta caaatcttca                 1750
atttatccgt cgcttcttaa ggtttttgtt gtttgttttg ttttattctg                 1800
aaacagggtc ccactgtgtt tgctgtggtt gccctggctg tctttgaact                 1850
tagagatctg cctctctctg ctcccctagt gccgggatca aaggcctgta                 1900
tctccaaact aatacttaat ctttaaattc tttttttttt tttttttttt                 1950
ttcgagacag ggttcctctg tatagccctg gctgtcctgg aactcacttt                 2000
gtagaccaga ctggcctcga actcagaaat ttgtctgcct ctgcctcccg                 2050
agtgctggga ttaaaggtgt gtgccaacac gcccggctca tctttaaatt                 2100
tttttataca aagtcaattg gtatcaaaat ctgatttgtc aatcgtggtg                 2150
gtacaaggca gatctctttt gagttcaagg ccagcctgat ctgcaagacc                 2200
agcttgatct ataagaccaa cctggtctat agcaagtttc agagtagcca                 2250
gggctacata gtgagacctt acctaaagat ccaaaccaaa taaaacagaa                 2300
ttcctgagtt aattactata caggcgctag agagatggtt caggcggtaa                 2350
aggcacttgc tgacaacctg agaacaatcc ctgggtccct tacgctagaa                 2400
ggagagaacc aactcctgca agttgtcctt ccacctctac atacaggttc                 2450
aggcatgcac acgtgtatac ccaaatgaat taaatacata gtagatacta                 2500
ggcaaaggtt tttgttgttg ttgttgtttg gttggttttg tttttttcga                 2550
gacagggttt ctctgtgtag ccctggctgt cctggaactc attctgtaga                 2600
ccaggctggc cttgaactca ggcctgcctc tgcctcccaa gtgctgggat                 2650
taaaggcgtg agccaccact gcctggcagc caagctcttt ttacagaagt                 2700
tgaaaacttg tgacttgtta tgaacaatag tcctcagcat tccaagaagt                 2750
tatgtgacct tactcaagtt gggcttacaa attagaagca ttttttgttt                 2800
tatagatctt tttagcatag taatttcaat ttaaaacatc acaaattgag                 2850
gactggagag acagttcatg ggtgaagagc actggctatt tttagaggcc                 2900
ccacattcgt ttcccagcac ccacatggtt gctcacagcc atctctaacc                 2950
ctagtcctaa gggccctgac accctcttct ggtctctggg tacactgcat                 3000
gcatgtggtg tatagaatac atgcagagtg gatcagtgtg acggacgaat                 3050
ggttttagtg actagttgtg tctagatcct attgcactga tggtcatgaa                 3100
gttgaaacat gggggaaaat gaactttata cccttcttca tgacttctgt                 3150
ccttttgcct gcctcctttc tcatctccta atattacagt cttggtttcc                 3200
tctctaaatt tttagacttt taaccctcac ctaaacctgt atcagctttt                 3250
ataaaaatct tttcaaaact tcacactgaa gcatctgcct ccaaaggttt                 3300
tgaatgtgaa cgtgggtaaa ctctgttctt tcaaatggcc catctcttat                 3350
tttttaattg ccctgtgtga gtctcaggac cactaagtct aacaggctgt                 3400
gaccagtgat tgtctctagg gcatctgagc ctcacagagt ctgggaagac                 3450
tgacaggagg aggtgaccca aggtctgtga gtgcaggctc cacccactgg                 3500
agctgagcac acacttggag gttccactta ccttagctct gccttcaggg                 3550
tctgacatgg ctcagcggat gacaacacag ctgctgctcc ttctagtgtg                 3600
ggtggctgta gtaggggagg ctcagacaag gattgcatgg gccaggactg                 3650
agcttctcaa tgtctgcatg aacgccaagc accacaagga aaagccaggc                 3700
cccgaggaca agttgcatga gcagtgtcga ccctggagga agaatgcctg                 3750
ctgttctacc aacaccagcc aggaagccca taaggatgtt tcctacctat                 3800
atagattcaa ctggaaccac tgtggagaga tggcacctgc ctgcaaacgg                 3850
catttcatcc aggacacctg cctctacgag tgctccccca acttggggcc                 3900
ctggatccag caggtggatc agagctggcg caaagagcgg gtactgaacg                 3950
tgcccctgtg caaagaggac tgtgagcaat ggtgggaaga ttgtcgcacc                 4000
tcctacacct gcaagagcaa ctggcacaag ggctggaact ggacttcagg                 4050
gtttaacaag tgcgcagtgg gagctgcctg ccaacctttc catttctact                 4100
tccccacacc cactgttctg tgcaatgaaa tctggactca ctcctacaag                 4150
gtcagcaact acagccgagg gagtggccgc tgcatccaga tgtggttcga                 4200
cccagcccag ggcaacccca atgaggaggt ggcgaggttc tatgctgcag                 4250
ccatgagtgg ggctgggccc tgggcagcct ggcctttcct gcttagcctg                 4300
gccctaatgc tgctgtggct gctcagctga tcctcaggtg caggctgcct                 4350
atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac                 4400
tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct                 4450
tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag                 4500
tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca                 4550
aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata                 4600
tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc                 4650
agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc                 4700
ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc                 4750
tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc                 4800
tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga                 4850
tcaagcttct cgagggacct aataacttcg tatagcatac attatacgaa                 4900
gttatattaa gggttccgca agctctagtc gagccccagc tggttctttc                 4950
cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt                 5000
cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata                 5050
gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga                 5100
aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg                 5150
ggcaaacaac agatggctgg caactagaag gcacagtcga ggctgatcag                 5200
cgagctctag agaattgatc ccctcagaag aactcgtcaa gaaggcgata                 5250
gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga                 5300
agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc                 5350
aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat                 5400
gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat                 5450
cgccatgggt cacgacgaga tcatcgccgt cgggcatgcg cgccttgagc                 5500
ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc                 5550
atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc                 5600
gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc                 5650
agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag                 5700
gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt                 5750
cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc                 5800
gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag                 5850
ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg                 5900
acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag                 5950
tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa                 6000
tccatcttgt tcaatggccg atcccatggt ttagttcctc accttgtcgt                 6050
attatactat gccgatatac tatgccgatg attaattgtc aacaggctgc                 6100
aggtcgaaag gcccggagat gaggaagagg agaacagcgc ggcagacgtg                 6150
cgcttttgaa gcgtgcagaa tgccgggcct ccggaggacc ttcgggcgcc                 6200
cgccccgccc ctgagcccgc ccctgagccc gcccccggac ccaccccttc                 6250
ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg                 6300
ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac                 6350
gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga                 6400
gctgcggggc gggggggaac ttcctgacta ggggaggagt agaaggtggc                 6450
gcgaaggggc caccaaagaa cggagccggt tggcgcctac cggtggatgt                 6500
ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgcccagcg                 6550
gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct                 6600
acccggtaga atttcgacga cctgcagcca agctagcttc gcgagctcga                 6650
ccgaacaaac gacccaacac ccgtgcgttt tattctgtct ttttattgcc                 6700
gctcagcttt acagtgacaa tgacggctgg cgactgaata ttagtgctta                 6750
cagacagcac tacatatttt ccgtcgatgt tgaaatcctt tctcatatgt                 6800
caccataaat atcaaataat tatagcaatc atttacgcgt taatggctaa                 6850
tcgccatctt ccagcaggcg caccattgcc cctgtttcac tatccaggtt                 6900
acggatatag ttcatgacaa tatttacatt ggtccagcca ccagcttgca                 6950
tgatctccgg tattgaaact ccagcgcggg ccatatctcg cgcggctccg                 7000
acacgggcac tgtgtccaga ccaggccagg tatctctgac cagagtcatc                 7050
cttagcgccg taaatcaatc gatgagttgc ttcaaaaatc ccttccaggg                 7100
cgcgagttga tagctggctg gtggcagatg gcgcggcaac accatttttt                 7150
ctgacccggc aaaacaggta gttattcgga tcatcagcta caccagagac                 7200
ggaaatccat cgctcgacca gtttagttac ccccaggcta agtgccttct                 7250
ctacacctgc ggtgctaacc agcgttttcg ttctgccaat atggattaac                 7300
attctcccac cgtcagtacg tgagatatct ttaaccctga tcctggcaat                 7350
ttcggctata cgtaacaggg tgttataagc aatccccaga aatgccagat                 7400
tacgtatatc ctggcagcga tcgctatttt ccatgagtga acgaacctgg                 7450
tcgaaatcag tgcgttcgaa cgctagagcc tgttttgcac gttcaccggc                 7500
atcaacgttt tcttttcgga tccgccgcat aaccagtgaa acagcattgc                 7550
tgtcacttgg tcgtggcagc ccggaccgac gatgaagcat gtttagctgg                 7600
cccaaatgtt gctggatagt ttttactgcc agaccgcgcg cctgaagata                 7650
tagaagataa tcgcgaacat cttcaggttc tgcgggaaac catttccggt                 7700
tattcaactt gcaccatgcc gcccacgacc ggcaaacgga cagaagcatt                 7750
ttccaggtat gctcagaaaa cgcctggcga tccctgaaca tgtccatcag                 7800
gttcttgcga acctcatcac tcgttgcatc gaccggtaat gcaggcaaat                 7850
tttggtgtac ggtcagtaaa ttggacacct tcctcttctt cttgggcatg                 7900
gccgcaggaa agcagagccc tgaagctccc atcaccggcc aataagagcc                 7950
aagcctgcag tgtgacctca tagagcaatg tgccagccag cctgacccca                 8000
agggccctca ggcttgggca cactgtctct aggaccctga gagaaagaca                 8050
tacccatttc tgcttagggc cctgaggatg agcccagggg tggcttggca                 8100
ctgaagcaaa ggacactggg gctcagctgg cagcaaagtg accaggatgc                 8150
tgaggctttg acccagaagc cagaggccag aggccaggac ttctcttggt                 8200
cccagtccac cctcactcag agctttacca atgccctctg gatagttgtc                 8250
gggtaacggt ggacgccact gattctctgg ccagcctagg acttcgccat                 8300
tccgctgatt ctgctcttcc agccactggc tgaccggttg gaagtactcc                 8350
agcagtgcct tggcatccag ggcatctgag cctaccaggt ccttcagtac                 8400
ctcctgccag ggcctggagc agccagcctg caacacctgc ctgccaagca                 8450
gagtgaccac tgtgggcaca ggggacacag ggtggggccc acaacagcac                 8500
cattgtccac ttgtccctca ctagtaaaag aactctaggg ttgcgggggg                 8550
tgggggaggt ctctgtgagg ctggtaaggg atatttgcct ggcccatgga                 8600
gctagcttgg ctggacgtaa actcctcttc agacctaata acttcgtata                 8650
gcatacatta tacgaagtta tattaagggt tattgaatat gatcggaatt                 8700
gggctgcagg aattcgatag cttggctgca ggtcgaccct aggtgtatgc                 8750
tgcccagtca cttttctgcc agcctttgaa gacttgagaa ggagactctc                 8800
atatctgaga agcctttgga ggcaggcatc tagcgaacac ttggactgtg                 8850
gtcctcagct tgagggctgg agggcttgag ggctctgtgt tataacagtt                 8900
gtttgccata gtgcttttag tatcccaaag ctcactaaac atttaataaa                 8950
atcagtgtga tgcaacaact atgaagtcaa ccagcagcag gtctgctatt                 9000
ggggaggtac aatcagtgca gacaacaaag tgggaggggg gtctcaaaaa                 9050
aagccaagat gagggctgga gagttggctc agtggttaaa agcacttgtt                 9100
gagcttgcag aataccaagg tctgatccac aacatccaag gtggtggatc                 9150
catccccttt caggggatgc aatgccctct tctgacctct gcaggcacca                 9200
ggtgtgcatg tgatacatat aaaataaata aagctaataa gtaaataagc                 9250
aaataggcct ggggatgtag ctcagctgat agagtacctg catggcagtc                 9300
acaaatctct agcaccatat ggaactggat gtgttgacac ataaatgtaa                 9350
cctcagccca ggggaggtag aggtagtcag ggtcagaagt tcaaggttac                 9400
cttcagctac atagtgagtt cagtgccagt ctggaatata tgaaaccctg                 9450
tcaagaaagg gatagagaga gggaggagag agggaggagg ctatttttat                 9500
tatttatttt ttgagacagg gttctactat gtaattctgg ctagcctgac                 9550
tctcacagag atctgtctgc ctcaatctcc tgagtgctag gattaaaggt                 9600
ggacatacca caccacaaaa aaatttaaaa aagcaaaaga aaagcaatct                 9650
gtatcatgtg catcaatgga taaatgagca tgttgactct attagctgag                 9700
agctgtcagg ttctatttgc tgctggtttt ttgtttattt gtttttatgt                 9750
atttattttt atggtatttt gcatgcatat ttgtcaatca aagaagtgca                 9800
gtgcatgcaa aggccagaag agggtgttgg agtctccacg accagagtta                 9850
taggtggttg tgagcctcca cgtgcatgct gggagctgaa tccaagtcct                 9900
ctgtaagaat gacaagtgct ctcaacccct gagccatcac tccaggctct                 9950
atttactttt tgtaatactt aagctaagca gtggtgatgc atgtgtttaa                 10000
tcctagcact tggaggcaga ggcagacgga tttctgagtt tgagatcagc                 10050
ctggtctaca tagtgaggtc caggatagtc agatctacac agagaaacca                 10100
tgttctaaat aaataaataa ataaataagc ctaaataaaa tttaaaaata                 10150
tttaagattt gtaccttgag aattccatat gtacatacaa tgtatttcca                 10200
tcatgtgtag ctcaggcaca cccacccttt gggttctact ttgtcttttc                 10250
ctcccacagg agcatggtca gcctatttga accacatttg actcttccct                 10300
gggaatcaca aactatcaat agatcctcag ctagggatga ggttcctgtg                 10350
tccttctcta acccactgct gctgctaatt tgttcgtttg tttgtgtctg                 10400
ggagacaggg tctctcttgt agcctgtctg gcctggaact caaacaggct                 10450
ggcctcccgg gataaaggat taaaggtgtg tgcctccata cctgtgctgc                 10500
tgttttgaaa cactgagcac aaggttgcta gttttagatt gagcagtggc                 10550
taccttgagc atccttaatt tacaaactta ttcaaacctt ccttccttcc                 10600
ttccttcctt ccttccttcc ttccttcctt ccttccttct tttctctctt                 10650
tttttaaaat ttatttttgt cacttgggag tcagaggcag gagaatccac                 10700
tgagccacag agctagcctg gtctacatag caagttccaa gtaagccaga                 10750
gctatatagt gagaccctgt atcaaaaata aatagccggg caatggtgat                 10800
gcacgccttt aatccaagca cttgggaggc agaggcaggc gaatttctga                 10850
gttcgagacc agcctagtct acagaatgag ttccaggaca gccaggacta                 10900
tacagagaaa ccctgtctcg aaaaaccaaa ccaaacaaaa aaaaaaaaaa                 10950
aaaggaaaga aagaaaaatt atatatatgc ttttgtttag tgtaggtgtg                 11000
tgtgtctgtg tgaatgaata ccacacatgt atgtatgagt tctggtggaa                 11050
ctaaaggaaa gcatcagatc ctttggagct ggagctgcag ccatatttga                 11100
gccaggacat aggtgctggg cagtagacta ttaaatgctg agtcatctct                 11150
ctagattctt agggtgtttt tgtttttgtt ttttaaactg ggcatggtgc                 11200
cacatgccct ttatcctagc actctgagaa gcagaggaaa acagatctct                 11250
gtgagttaga ggccagtatg atccacatag caaatttcag ggttatccca                 11300
gtctcaaaaa caaacaaaca aacaaacaaa caaacaaaca aacagaataa                 11350
aacaacaaca aaaaacttgt tttgaaggtg tgttttgaaa ctttgttatg                 11400
tgctaagttc caggccaaga gtcgattttc aggagtaagt tagcgtattg                 11450
cgagtgttct ttttctcggt gacatttaat gcgaccatct ggtggcactg                 11500
caagtccaca tgcctttctc ttgcttttat ttatttattt atttatttat                 11550
ttatttcccc aaagcgattg gcatgttggg tttatcattt tatcccttat                 11600
ttcctaggaa agtggcttgg ggtgggtcgg ggtctcataa agactagaat                 11650
caaggggctg gagagatggc tcagcagtta agagcaccta ctgctcttct                 11700
gaaggtcatg agttcaaatc ctagcaacca catgatggct tacaaccatc                 11750
cgtaatgaga tctgatgccc tcttctaggg tgtctgaaga cagctactgt                 11800
gtacttacat ataataaata aataaatctt aaaaaaaaaa caaaacaaaa                 11850
ctagaattaa agaactagat gtatgatcag ctgtggtggt ggtaagccta                 11900
cctctgggga tgctaggtta acctgatcta tatagtgagt tacaggccag                 11950
ccagggttac ctagtgaaaa cctggctttt taaaatttta attaactaag                 12000
gaaagttaaa cctggatgtg gtagcacgca tctcaccact tgagaagcag                 12050
aagagattca ggagtccaag gctatccttg gttacatata gagtctgaag                 12100
ccacttagac tctatgaggc tgctcagaaa aaagggaccg ggaagacaac                 12150
acttgcctca gaaccataag gacccgagtt taatccttag gactcatgta                 12200
caaatgccta gggtaatgtg ggtttatgac ctcagcaccg aggagataag                 12250
atcagtgagt ccgaatgttt atcaaaggtt agaatagctt catttgtggt                 12300
ccccaggcca gtgagagacc ctgcctcaaa gtgggcagac tgcctgaggt                 12350
tgtcttctga cctctacact tgcaaacaaa tgtgtgtgtg aacactcata                 12400
gatacacaaa taggcacaca cacacacaaa gcacatgtgc aaagaaaaaa                 12450
ataccaaggc atagtgagtg gcccatgcct ttaattctag cacttagaag                 12500
gcagaggcag tcagatagct tggagtttaa ttcaagtctg gtttacatag                 12550
tgaattacag acagttgtga gcctccattg tgggtgcttg aaaccaaacc                 12600
ctggtcttct gcaagagcaa ccagtgtgct cttacccact gagccagatg                 12650
tctcttctgg gcttctacag ccagcttaac agcaagtatc gaaatggcta                 12700
ggcaatcaag gactcttcaa agtgcaaggc tgcatgtgga atattctgtc                 12750
tgtgcttggc cacggtcctg tgatttccat ctcacagtgc agcccctgga                 12800
agacgaattc ctgctgttcc acgaacacaa gccaggaagc acataaggac                 12850
atttcctacc tgtaccggtt caactggaac cactgcggaa ctatgacatc                 12900
ggaatgcaaa cggcacttta tccaagacac ctgcctctat gagtgttccc                 12950
cgaacttggg accctggatc cagcaggtac gagtgtcccc cagtgccagc                 13000
ggagtagctg tgctctccaa atctctacag gctatgtctg cagctccgat                 13050
tctactcagg ctgcatttct gaggttcctg agttcaaaac cttttacacc                 13100
ctctctcagg tggaccagag ctggcgcaaa gagcggatcc ttgatgttcc                 13150
cctgtgcaaa gaggactgtc agcagtggtg ggaggactgc cagagctctt                 13200
ttacctgcaa gagcaattgg cacaagggat ggaactggtc ctcgggtaag                 13250
gagtaggtgg agtgggcgcg gccgcgtacc agcttttgtt ccctttagtg                 13300
agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt                 13350
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata                 13400
aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc                 13450
gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc                 13500
attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc                 13550
tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg                 13600
gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat                 13650
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc                 13700
aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc                 13750
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc                 13800
cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg                 13850
cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct                 13900
cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca                 13950
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc                 14000
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa                 14050
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga                 14100
ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg                 14150
cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct                 14200
gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac                 14250
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg                 14300
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc                 14350
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat                 14400
tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt                 14450
aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg                 14500
cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca                 14550
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta                 14600
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc                 14650
tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa                 14700
gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg                 14750
gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc                 14800
cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat                 14850
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg                 14900
tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa                 14950
gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc                 15000
ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca                 15050
accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc                 15100
ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc                 15150
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg                 15200
ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc                 15250
agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc                 15300
aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc                 15350
atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct                 15400
catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg                 15450
ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc                 15500
gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact                 15550
tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg                 15600
ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta                 15650
gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta                 15700
gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc                 15750
ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact                 15800
ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat                 15850
tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat                 15900
ttaacgcgaa ttttaacaaa atattaa                                          15927

<210>  60
<211>  134
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Non-targeting control VHH R3B23

<400>  60
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Asp Thr Gly Tyr 
                20                  25                  30  

Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Gly Val 
                35                  40                  45  

Ala Val Ile Asn Ser Asp Ser Gly Val Gly Ser Thr Tyr Tyr Ala 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Ile Tyr Tyr Cys Ala Ala Gly His Phe Ser Asp Tyr Val Ser 
                95                  100                 105 

Pro Trp Thr Trp Arg Glu Ile Tyr Arg Tyr Asn Val Trp Gly Gln 
                110                 115                 120 

Gly Thr Gln Val Thr Val Ser Ser His His His His His His 
                125                 130                 

<210>  61
<211>  80
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A3: N37-W118 (IMGT)

<400>  61
Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg Asp 
1               5                   10                  15  

Trp Val Ala Val Ile Thr Lys Asp Gly Ser Thr Asn Tyr Ala Asp 
                20                  25                  30  

Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                35                  40                  45  

Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala 
                50                  55                  60  

Val Tyr Tyr Cys Asn Val Gln Asp Gly Ser Gly Trp Ile Arg Ala 
                65                  70                  75  

Trp Gly Trp Asp Trp 
                80  

<210>  62
<211>  30
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A3: N37-D69 (IMGT)

<400>  62
Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg Asp 
1               5                   10                  15  

Trp Val Ala Val Ile Thr Lys Asp Gly Ser Thr Asn Tyr Ala Asp 
                20                  25                  30  




<210>  63
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A3: N105-W118 (IMGT)

<400>  63
Asn Val Gln Asp Gly Ser Gly Trp Ile Arg Ala Trp Gly Trp Asp 
1               5                   10                  15  

Trp 
    

<210>  64
<211>  76
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A4: A38-W118 (IMGT)

<400>  64
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
1               5                   10                  15  

Val Ala Asp Ile Asn Trp Ser Glu Gly Ser Pro Met Tyr Ile Asp 
                20                  25                  30  

Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn 
                35                  40                  45  

Ser Leu Tyr Leu Gln Met Asn Ser Leu Lys Ser Glu Asp Thr Ala 
                50                  55                  60  

Val Tyr Tyr Cys Ala Ala Arg Arg Phe Gly Gln Gly Tyr Glu Tyr 
                65                  70                  75  

Trp 
    

<210>  65
<211>  30
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A4: A38-D69 (IMGT)

<400>  65
Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
1               5                   10                  15  

Val Ala Asp Ile Asn Trp Ser Glu Gly Ser Pro Met Tyr Ile Asp 
                20                  25                  30  




<210>  66
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A4: A105-W118 (IMGT)

<400>  66
Ala Ala Arg Arg Phe Gly Gln Gly Tyr Glu Tyr Trp 
1               5                   10          

<210>  67
<211>  80
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A5: N37-W118 (IMGT)

<400>  67
Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg Glu 
1               5                   10                  15  

Trp Val Ala Leu Ile Thr Lys Asp Gly Ser Thr Asn Tyr Glu Asp 
                20                  25                  30  

Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                35                  40                  45  

Thr Val Ser Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala 
                50                  55                  60  

Val Tyr Tyr Cys Asn Ala Gln Asp Gly Ser Gly Trp Ile Arg Ala 
                65                  70                  75  

Trp Gly Trp Asp Trp 
                80  

<210>  68
<211>  30
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A5: N37-D69 (IMGT)

<400>  68
Asn Leu Met Gly Trp Tyr Arg Gln Pro Pro Gly Lys Glu Arg Glu 
1               5                   10                  15  

Trp Val Ala Leu Ile Thr Lys Asp Gly Ser Thr Asn Tyr Glu Asp 
                20                  25                  30  




<210>  69
<211>  16
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Part of A5: N105-W118 (IMGT)

<400>  69
Asn Ala Gln Asp Gly Ser Gly Trp Ile Arg Ala Trp Gly Trp Asp 
1               5                   10                  15  

Trp 
    

<210>  70
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  D59E variant of A1

<400>  70
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Glu Gly Asn Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115                 

<210>  71
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N64E variant of A1

<400>  71
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Asp Gly Glu Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115                 

<210>  72
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N64Q variant of A1

<400>  72
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Asp Gly Gln Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115                 

<210>  73
<211>  119
<212>  PRT
<213>  Artificial sequence

<220>
<223>  N64S variant of A1

<400>  73
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Glu Ser Ile Phe Ser 
                20                  25                  30  

Arg Asn His Met Ser Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Asp Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Thr Phe Gly Arg Ser Val Ala Gly Phe 
                95                  100                 105 

Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115                 

<210>  74
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  E90Q variant of A2

<400>  74
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Met Lys Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115             

<210>  75
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  K29Q-E90Q variant of A2

<400>  75
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Met Gln Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115             

<210>  76
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  K29R-E90Q variant of A2

<400>  76
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Met Arg Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115             

<210>  77
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  M28A-E90Q variant of A2

<400>  77
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Ala Lys Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115             

<210>  78
<211>  118
<212>  PRT
<213>  Artificial sequence

<220>
<223>  M28T-E90Q variant of A2

<400>  78
Asp Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly 
1               5                   10                  15  

Gly Ser Leu Arg Leu Ser Cys Ala Ala Asp Glu Thr Lys Phe Ser 
                20                  25                  30  

Asn Tyr Asp Leu Ala Trp Tyr Arg Arg Ala Pro Gly Lys Gln Arg 
                35                  40                  45  

Glu Leu Ala Ala Ile Ile Thr Ser Gly Gly Ser Thr Asn Tyr Pro 
                50                  55                  60  

Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys 
                65                  70                  75  

Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr 
                80                  85                  90  

Ala Val Tyr Tyr Cys Asn Ala Leu Arg Pro Trp Pro Gln Phe Ser 
                95                  100                 105 

Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
                110                 115             

<210>  79
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  hemaglutinin tag (HA-tag)

<400>  79
Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   

<210>  80
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  tag

<400>  80
Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser 
1               5                   10  

<210>  81
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  non-limiting example of a cysteine tag

<400>  81
Ser Pro Ser Thr Pro Pro Thr Pro Ser Pro Ser Thr Pro Pro Cys 
1               5                   10                  15  




<210>  82
<211>  357
<212>  DNA
<213>  Artificial sequence

<220>
<223>  coding for A1

<400>  82
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagc ctggggggtc                 50
tctgagactc tcctgtgcag cctctgaaag catcttcagt cgcaatcaca                 100
tgagctggta ccgccaggct ccagggaagc agcgcgagtt ggccgcgatt                 150
attactagtg atggtaacac aaactatcca gactccgtga agggccgatt                 200
caccatctcc agagacaacg ctaagaacac ggtgtatctg caaatgaaca                 250
gcctgaaacc tgaggacacg gccgtctatt actgtaatac cttcggtcgt                 300
agcgtagctg gtttttatga ctactggggc caggggaccc aggtcaccgt                 350
ctcctca                                                                357

<210>  83
<211>  354
<212>  DNA
<213>  Artificial sequence

<220>
<223>  coding for A2

<400>  83
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagg ctggggggtc                 50
tctgagactc tcctgtgcag ccgatgaaat gaaattcagt aattatgact                 100
tggcctggta ccgccgggct ccagggaagc agcgcgagtt ggccgcaatt                 150
attactagtg gtggtagcac aaactatccg gactccgtga agggccgatt                 200
caccatctcc agagacaacg ccaagaacac ggtgtatcta gaaatgaaca                 250
gcctgaaacc tgaggacaca gccgtctatt actgcaatgc acttcgaccc                 300
tggcctcaat ttagcgacta ctggggccag gggacccagg tcaccgtctc                 350
ctca                                                                   354

<210>  84
<211>  363
<212>  DNA
<213>  Artificial sequence

<220>
<223>  coding for A3

<400>  84
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagg ctggggggtc                 50
tctgagactc tcctgtggag tctctgggaa catgttcagt atgaatctca                 100
tgggctggta ccgccaacct ccagggaagg agcgcgactg ggtcgcagtt                 150
attactaaag atgggagcac aaactatgca gactccgtga agggccgatt                 200
caccatctcc agggacaacg ccaagaacac ggtgtctctg caaatgaaca                 250
gcctgaaacc tgaggacaca gccgtctact actgtaatgt ccaggatggt                 300
agtggttgga ttcgggcgtg gggctgggac tggggccagg gaacccaggt                 350
caccgtctcc tca                                                         363

<210>  85
<211>  357
<212>  DNA
<213>  Artificial sequence

<220>
<223>  coding for A4

<400>  85
gatgtgcagc tggtggagtc tggaggagga ttggccaggg ctgggggctc                 50
tctgagactc tcctgtgcag cctctggacg cgccttcagt agtacgtatg                 100
ccatgggttg gttccgccag gctccaggga aggagcgtga gtttgtagcg                 150
gatattaatt ggagtgaagg tagcccgatg tatatagatt ccgtgaagga                 200
ccgattcacc atctccagag acaacgccag aaattcgctg tatcttcaga                 250
tgaacagcct gaaatctgag gacacggccg tttattactg tgcagcgcgg                 300
aggttcggtc aggggtatga gtactggggc caggggaccc aggtcaccgt                 350
ctcctca                                                                357

<210>  86
<211>  363
<212>  DNA
<213>  Artificial sequence

<220>
<223>  coding for A5

<400>  86
gatgtgcagc tggtggagtc tgggggaggc ttggtgcagg ctggggggtc                 50
tctgagactc tcctgtggag tctctgggaa catcttcagt atgaatctca                 100
tgggctggta ccgccaacct ccagggaagg agcgcgagtg ggtcgcacta                 150
attactaaag atgggagcac aaattatgaa gactccgtga agggccgatt                 200
caccatctcc agggacaacg ccaagaacac ggtgtctctg caaatgaaca                 250
gcctgaaacc tgaggacaca gccgtctact actgtaatgc ccaggatggt                 300
agtggctgga ttcgggcgtg gggctgggac tggggccagg gaacccaggt                 350
caccgtctcc tca                                                         363



