                         SEQUENCE LISTING

<110>  The Wistar Institute of Anatomy and Biology
       Parzych, Liz
       Weiner, David
 
<120>  DNA ANTIBODY CONSTRUCTS FOR USE AGAINST ROTAVIRUS

<130>  206193-0067-00WO

<150>  US 63/050,275
<151>  2020-07-10

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAB 41 VH

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Pro Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Glu Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Thr Ser Pro Ala Ile Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Ser Leu Tyr 
65                  70                  75                  80  


Leu His Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Ile Ser His Gln Gln Phe Ser Ser Gly Trp Asn Ala Trp Phe 
            100                 105                 110         


Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  2
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAB 41 VL

<400>  2

Asn Phe Met Leu Thr Gln Pro His Ser Val Ser Glu Ser Pro Gly Lys 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Thr Gly Ser Ser Gly Ser Ile Ala Ser Asn 
            20                  25                  30          


Tyr Val Gln Trp Tyr Arg Gln Arg Pro Gly Ser Ala Pro Thr Thr Val 
        35                  40                  45              


Ile Tyr Glu Asn Tyr Gln Arg Pro Ser Gly Val Pro Ala Arg Phe Ser 
    50                  55                  60                  


Gly Ser Ile Asp Arg Ser Ser Asn Ser Ala Ser Leu Thr Ile Ser Gly 
65                  70                  75                  80  


Leu Gln Thr Asp Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asn 
                85                  90                  95      


Asn Asn Leu Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110     


<210>  3
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified HC (VH MOD)

<400>  3

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Glu Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Tyr Ile Ser Thr Ser Pro Ala Ile Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Ile Ser His Gln Gln Phe Ser Ser Gly Trp Asn Ala Trp Phe 
            100                 105                 110         


Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  4
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Modified LC (VL MOD)

<400>  4

Asn Phe Met Leu Thr Gln Pro His Ser Val Ser Glu Ser Pro Gly Lys 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Thr Gly Ser Ser Gly Ser Ile Ala Ser Asn 
            20                  25                  30          


Tyr Val Gln Trp Tyr Gln Gln Arg Pro Gly Ser Ser Pro Thr Thr Val 
        35                  40                  45              


Ile Tyr Glu Asn Tyr Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser 
    50                  55                  60                  


Gly Ser Ile Asp Ser Ser Ser Asn Ser Ala Ser Leu Thr Ile Ser Gly 
65                  70                  75                  80  


Leu Lys Thr Glu Asp Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asn 
                85                  90                  95      


Asn Asn Leu Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 110     


<210>  5
<211>  763
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb41_HC/LC_WT: AA

<400>  5

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Pro Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asn Tyr Glu Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Tyr Ile Ser Thr Ser Pro Ala Ile Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser 
                85                  90                  95      


Ser Leu Tyr Leu His Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Ile Ser His Gln Gln Phe Ser Ser Gly Trp Asn 
        115                 120                 125             


Ala Trp Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Ser Ser Glu Ser Ala Arg Asn Pro Thr Ile Tyr Pro Leu Thr Leu Pro 
145                 150                 155                 160 


Pro Ala Leu Ser Ser Asp Pro Val Ile Ile Gly Cys Leu Ile His Asp 
                165                 170                 175     


Tyr Phe Pro Ser Gly Thr Met Asn Val Thr Trp Gly Lys Ser Gly Lys 
            180                 185                 190         


Asp Ile Thr Thr Val Asn Phe Pro Pro Ala Leu Ala Ser Gly Gly Arg 
        195                 200                 205             


Tyr Thr Met Ser Ser Gln Leu Thr Leu Pro Ala Val Glu Cys Pro Glu 
    210                 215                 220                 


Gly Glu Ser Val Lys Cys Ser Val Gln His Asp Ser Asn Pro Val Gln 
225                 230                 235                 240 


Glu Leu Asp Val Asn Cys Ser Gly Pro Thr Pro Pro Pro Pro Ile Thr 
                245                 250                 255     


Ile Pro Ser Cys Gln Pro Ser Leu Ser Leu Gln Arg Pro Ala Leu Glu 
            260                 265                 270         


Asp Leu Leu Leu Gly Ser Asp Ala Ser Ile Thr Cys Thr Leu Asn Gly 
        275                 280                 285             


Leu Arg Asn Pro Glu Gly Ala Val Phe Thr Trp Glu Pro Ser Thr Gly 
    290                 295                 300                 


Lys Asp Ala Val Gln Lys Lys Ala Val Gln Asn Ser Cys Gly Cys Tyr 
305                 310                 315                 320 


Ser Val Ser Ser Val Leu Pro Gly Cys Ala Glu Arg Trp Asn Ser Gly 
                325                 330                 335     


Ala Ser Phe Lys Cys Thr Val Thr His Pro Glu Ser Gly Thr Leu Thr 
            340                 345                 350         


Gly Thr Ile Ala Lys Val Thr Val Asn Thr Phe Pro Pro Gln Val His 
        355                 360                 365             


Leu Leu Pro Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Leu Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Arg Ala Phe Asn Pro Lys Glu Val Leu Val Arg 
385                 390                 395                 400 


Trp Leu His Gly Asn Glu Glu Leu Ser Pro Glu Ser Tyr Leu Val Phe 
                405                 410                 415     


Glu Pro Leu Lys Glu Pro Gly Glu Gly Ala Thr Thr Tyr Leu Val Thr 
            420                 425                 430         


Ser Val Leu Arg Val Ser Ala Glu Thr Trp Lys Gln Gly Asp Gln Tyr 
        435                 440                 445             


Ser Cys Met Val Gly His Glu Ala Leu Pro Met Asn Phe Thr Gln Lys 
    450                 455                 460                 


Thr Ile Asp Arg Leu Ser Gly Lys Pro Thr Asn Val Ser Val Ser Val 
465                 470                 475                 480 


Ile Met Ser Glu Gly Asp Gly Ile Cys Tyr Arg Gly Arg Lys Arg Arg 
                485                 490                 495     


Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
            500                 505                 510         


Val Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu Leu Leu Pro 
        515                 520                 525             


Leu Leu Thr Phe Cys Thr Val Ser Glu Ala Ser Asn Phe Met Leu Thr 
    530                 535                 540                 


Gln Pro His Ser Val Ser Glu Ser Pro Gly Lys Thr Val Thr Ile Ser 
545                 550                 555                 560 


Cys Thr Gly Ser Ser Gly Ser Ile Ala Ser Asn Tyr Val Gln Trp Tyr 
                565                 570                 575     


Arg Gln Arg Pro Gly Ser Ala Pro Thr Thr Val Ile Tyr Glu Asn Tyr 
            580                 585                 590         


Gln Arg Pro Ser Gly Val Pro Ala Arg Phe Ser Gly Ser Ile Asp Arg 
        595                 600                 605             


Ser Ser Asn Ser Ala Ser Leu Thr Ile Ser Gly Leu Gln Thr Asp Asp 
    610                 615                 620                 


Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asn Asn Asn Leu Trp Val 
625                 630                 635                 640 


Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Thr Lys Leu Thr Val 
                645                 650                 655     


Leu Gly Gln Pro Lys Ser Ser Pro Ser Val Thr Leu Phe Pro Pro Ser 
            660                 665                 670         


Ser Glu Glu Leu Glu Thr Asn Lys Ala Thr Leu Val Cys Thr Ile Thr 
        675                 680                 685             


Asp Phe Tyr Pro Gly Val Val Thr Val Asp Trp Lys Val Asp Gly Thr 
    690                 695                 700                 


Pro Val Thr Gln Gly Met Glu Thr Thr Gln Pro Ser Lys Gln Ser Asn 
705                 710                 715                 720 


Asn Lys Tyr Met Ala Ser Ser Tyr Leu Thr Leu Thr Ala Arg Ala Trp 
                725                 730                 735     


Glu Arg His Ser Ser Tyr Ser Cys Gln Val Thr His Glu Gly His Thr 
            740                 745                 750         


Val Glu Lys Ser Leu Ser Arg Ala Asp Cys Ser 
        755                 760             


<210>  6
<211>  2279
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mAb41_HC/LC_WT: Nucleic Acid

<400>  6
atggactgga cctggagaat cctgttcctg gtggctgctg ctaccggaac acacgctgag       60

gtgcagctgg tggagagcgg cggaggccca gtgcagcccg gaggctccct gaagctgtct      120

tgcgctgcag cggattcacc ttttctaact acgagatgta ctgggtgcgg caggctccag      180

gcaagggact ggagtgggtg agctacatct ctacaagccc tgctatcacc tactacgctg      240

actccgtgcg gggccgcttt acatctctcg cgataacgct aagagctccc tgtacctgca      300

catgaacagc ctgagggctg aggacacagc cgtgtactac tgcgctacca tctcccacca      360

gcagttctct agcggctgga acgcctggtt tgatccatgg gccagggaac actggtgacc      420

gtgtcctcta gctccgagag cgcccgcaac cctacaatct acccactgac cctgccccct      480

gccctgtcta gcgaccctgt gatcatcggc tgtctgatcc acgattactt cccaagcgga      540

caatgaacgt gacctggggc aagtccggaa aggacatcac cacagtgaac tttccacccg      600

ctctggcttc cggaggcagg tacacaatgt cctctcagct gaccctgcca gctgtggagt      660

gccctgaggg agagtccgta agtgttctgt gcagcacgac tccaacccag tgcaggagct      720

ggatgtgaac tgctctggac ccacacctcc accccctatc accatcccca gctgtcagcc      780

ttccctgtct ctgcagagac ccgctctgga ggacctctgc tgggctccga tgcctctatc      840

acctgcacac tgaacggcct gcggaaccct gagggagccg tgttcacatg ggagccatcc      900

accggcaagg atgctgtgca gaagaaggcc gtgcagaaca gctgcggatg ttactcgtga      960

gctccgtgct gccaggatgc gctgagagat ggaacagcgg agcctccttc aagtgtaccg     1020

tgacacaccc tgagagcggc accctgacag gaaccatcgc caaggtgaca gtgaacacct     1080

ttccacccca gggcacctgc tgcctccacc ctctgaggag ctggctctga acgagctgct     1140

gagcctgacc tgtctggtga gagccttcaa ccctaaggag gtgctggtgc ggtggctgca     1200

cggcaacgag gagctgtctc cagagagcac ctggtgtttg agccactgaa ggagcccggc     1260

gagggagcta ccacatacct ggtgacatct gtgctgcgcg tgagcgccga gacctggaag     1320

cagggcgacc agtactcctg catggtggga cacgaggccc tgccatgaac ttcacacaga     1380

agaccatcga caggctgagc ggcaagccca ccaacgtgag cgtgtccgtg atcatgtccg     1440

agggcgatgg aatctgttac aggggaagaa agaggagatc tggcagcgga gctacaaact     1500

tagcctgctg aagcaggctg gcgatgtgga ggagaaccca ggacctatgg cctggacccc     1560

tctgctgctg ccactgctga cattctgcac cgtgtccgag gcttctaact ttatgctgac     1620

acagccacac tccgttctga gagccccggc aagacagtga ccatctcctg taccggctct     1680

agcggaagca tcgcctccaa ctacgtgcag tggtacaggc agaggccagg atctgctcct     1740

accacagtga tctacgagaa ctaccagagg ccagcggagt gccagctcgc ttctccggat     1800

ctatcgacag gtcctctaac agcgcctccc tgacaatctc cggcctgcag accgacgatg     1860

aggctgacta ctactgccag tcttacgata acaacaacct gtgggtgttt gaggcggaac     1920

aaagctgacc gtgctgggca ccaaactgac cgtgctggga cagcctaaga gctccccaag     1980

cgtgacactg ttccctccat ctagcgagga gctggagaca aacaaggcca ccctggtgtg     2040

cacaatcacg acttttaccc tggcgtggtg accgtggact ggaaggtgga tggcacaccc     2100

gtgacccagg gaatggagac cacacagcct tctaagcaga gcaacaacaa gtacatggcc     2160

tcctcttacc tgacactgac cgctaggcct gggagagaca cagctcctac tcctgccagg     2220

tgacacacga gggccacacc gtggagaagt ctctgagccg ggctgattgt tcttgataa      2279


<210>  7
<211>  763
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb41_HC/LC_MOD: AA

<400>  7

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asn Tyr Glu Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Tyr Ile Ser Thr Ser Pro Ala Ile Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                85                  90                  95      


Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Ile Ser His Gln Gln Phe Ser Ser Gly Trp Asn 
        115                 120                 125             


Ala Trp Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Ser Ser Glu Ser Ala Arg Asn Pro Thr Ile Tyr Pro Leu Thr Leu Pro 
145                 150                 155                 160 


Pro Ala Leu Ser Ser Asp Pro Val Ile Ile Gly Cys Leu Ile His Asp 
                165                 170                 175     


Tyr Phe Pro Ser Gly Thr Met Asn Val Thr Trp Gly Lys Ser Gly Lys 
            180                 185                 190         


Asp Ile Thr Thr Val Asn Phe Pro Pro Ala Leu Ala Ser Gly Gly Arg 
        195                 200                 205             


Tyr Thr Met Ser Ser Gln Leu Thr Leu Pro Ala Val Glu Cys Pro Glu 
    210                 215                 220                 


Gly Glu Ser Val Lys Cys Ser Val Gln His Asp Ser Asn Pro Val Gln 
225                 230                 235                 240 


Glu Leu Asp Val Asn Cys Ser Gly Pro Thr Pro Pro Pro Pro Ile Thr 
                245                 250                 255     


Ile Pro Ser Cys Gln Pro Ser Leu Ser Leu Gln Arg Pro Ala Leu Glu 
            260                 265                 270         


Asp Leu Leu Leu Gly Ser Asp Ala Ser Ile Thr Cys Thr Leu Asn Gly 
        275                 280                 285             


Leu Arg Asn Pro Glu Gly Ala Val Phe Thr Trp Glu Pro Ser Thr Gly 
    290                 295                 300                 


Lys Asp Ala Val Gln Lys Lys Ala Val Gln Asn Ser Cys Gly Cys Tyr 
305                 310                 315                 320 


Ser Val Ser Ser Val Leu Pro Gly Cys Ala Glu Arg Trp Asn Ser Gly 
                325                 330                 335     


Ala Ser Phe Lys Cys Thr Val Thr His Pro Glu Ser Gly Thr Leu Thr 
            340                 345                 350         


Gly Thr Ile Ala Lys Val Thr Val Asn Thr Phe Pro Pro Gln Val His 
        355                 360                 365             


Leu Leu Pro Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Leu Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Arg Ala Phe Asn Pro Lys Glu Val Leu Val Arg 
385                 390                 395                 400 


Trp Leu His Gly Asn Glu Glu Leu Ser Pro Glu Ser Tyr Leu Val Phe 
                405                 410                 415     


Glu Pro Leu Lys Glu Pro Gly Glu Gly Ala Thr Thr Tyr Leu Val Thr 
            420                 425                 430         


Ser Val Leu Arg Val Ser Ala Glu Thr Trp Lys Gln Gly Asp Gln Tyr 
        435                 440                 445             


Ser Cys Met Val Gly His Glu Ala Leu Pro Met Asn Phe Thr Gln Lys 
    450                 455                 460                 


Thr Ile Asp Arg Leu Ser Gly Lys Pro Thr Asn Val Ser Val Ser Val 
465                 470                 475                 480 


Ile Met Ser Glu Gly Asp Gly Ile Cys Tyr Arg Gly Arg Lys Arg Arg 
                485                 490                 495     


Ser Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
            500                 505                 510         


Val Glu Glu Asn Pro Gly Pro Met Ala Trp Thr Pro Leu Leu Leu Pro 
        515                 520                 525             


Leu Leu Thr Phe Cys Thr Val Ser Glu Ala Ser Asn Phe Met Leu Thr 
    530                 535                 540                 


Gln Pro His Ser Val Ser Glu Ser Pro Gly Lys Thr Val Thr Ile Ser 
545                 550                 555                 560 


Cys Thr Gly Ser Ser Gly Ser Ile Ala Ser Asn Tyr Val Gln Trp Tyr 
                565                 570                 575     


Gln Gln Arg Pro Gly Ser Ser Pro Thr Thr Val Ile Tyr Glu Asn Tyr 
            580                 585                 590         


Gln Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Ile Asp Ser 
        595                 600                 605             


Ser Ser Asn Ser Ala Ser Leu Thr Ile Ser Gly Leu Lys Thr Glu Asp 
    610                 615                 620                 


Glu Ala Asp Tyr Tyr Cys Gln Ser Tyr Asp Asn Asn Asn Leu Trp Val 
625                 630                 635                 640 


Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly Thr Lys Leu Thr Val 
                645                 650                 655     


Leu Gly Gln Pro Lys Ser Ser Pro Ser Val Thr Leu Phe Pro Pro Ser 
            660                 665                 670         


Ser Glu Glu Leu Glu Thr Asn Lys Ala Thr Leu Val Cys Thr Ile Thr 
        675                 680                 685             


Asp Phe Tyr Pro Gly Val Val Thr Val Asp Trp Lys Val Asp Gly Thr 
    690                 695                 700                 


Pro Val Thr Gln Gly Met Glu Thr Thr Gln Pro Ser Lys Gln Ser Asn 
705                 710                 715                 720 


Asn Lys Tyr Met Ala Ser Ser Tyr Leu Thr Leu Thr Ala Arg Ala Trp 
                725                 730                 735     


Glu Arg His Ser Ser Tyr Ser Cys Gln Val Thr His Glu Gly His Thr 
            740                 745                 750         


Val Glu Lys Ser Leu Ser Arg Ala Asp Cys Ser 
        755                 760             


<210>  8
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb41_HC_WT: AA

<400>  8

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Pro Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asn Tyr Glu Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Tyr Ile Ser Thr Ser Pro Ala Ile Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Arg Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser 
                85                  90                  95      


Ser Leu Tyr Leu His Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Ile Ser His Gln Gln Phe Ser Ser Gly Trp Asn 
        115                 120                 125             


Ala Trp Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Ser Ser Glu Ser Ala Arg Asn Pro Thr Ile Tyr Pro Leu Thr Leu Pro 
145                 150                 155                 160 


Pro Ala Leu Ser Ser Asp Pro Val Ile Ile Gly Cys Leu Ile His Asp 
                165                 170                 175     


Tyr Phe Pro Ser Gly Thr Met Asn Val Thr Trp Gly Lys Ser Gly Lys 
            180                 185                 190         


Asp Ile Thr Thr Val Asn Phe Pro Pro Ala Leu Ala Ser Gly Gly Arg 
        195                 200                 205             


Tyr Thr Met Ser Ser Gln Leu Thr Leu Pro Ala Val Glu Cys Pro Glu 
    210                 215                 220                 


Gly Glu Ser Val Lys Cys Ser Val Gln His Asp Ser Asn Pro Val Gln 
225                 230                 235                 240 


Glu Leu Asp Val Asn Cys Ser Gly Pro Thr Pro Pro Pro Pro Ile Thr 
                245                 250                 255     


Ile Pro Ser Cys Gln Pro Ser Leu Ser Leu Gln Arg Pro Ala Leu Glu 
            260                 265                 270         


Asp Leu Leu Leu Gly Ser Asp Ala Ser Ile Thr Cys Thr Leu Asn Gly 
        275                 280                 285             


Leu Arg Asn Pro Glu Gly Ala Val Phe Thr Trp Glu Pro Ser Thr Gly 
    290                 295                 300                 


Lys Asp Ala Val Gln Lys Lys Ala Val Gln Asn Ser Cys Gly Cys Tyr 
305                 310                 315                 320 


Ser Val Ser Ser Val Leu Pro Gly Cys Ala Glu Arg Trp Asn Ser Gly 
                325                 330                 335     


Ala Ser Phe Lys Cys Thr Val Thr His Pro Glu Ser Gly Thr Leu Thr 
            340                 345                 350         


Gly Thr Ile Ala Lys Val Thr Val Asn Thr Phe Pro Pro Gln Val His 
        355                 360                 365             


Leu Leu Pro Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Leu Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Arg Ala Phe Asn Pro Lys Glu Val Leu Val Arg 
385                 390                 395                 400 


Trp Leu His Gly Asn Glu Glu Leu Ser Pro Glu Ser Tyr Leu Val Phe 
                405                 410                 415     


Glu Pro Leu Lys Glu Pro Gly Glu Gly Ala Thr Thr Tyr Leu Val Thr 
            420                 425                 430         


Ser Val Leu Arg Val Ser Ala Glu Thr Trp Lys Gln Gly Asp Gln Tyr 
        435                 440                 445             


Ser Cys Met Val Gly His Glu Ala Leu Pro Met Asn Phe Thr Gln Lys 
    450                 455                 460                 


Thr Ile Asp Arg Leu Ser Gly Lys Pro Thr Asn Val Ser Val Ser Val 
465                 470                 475                 480 


Ile Met Ser Glu Gly Asp Gly Ile Cys Tyr 
                485                 490 


<210>  9
<211>  1466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mAb41_HC_WT: Nucleic Acid

<400>  9
atggactgga cctggagaat cctgttcctg gtggctgctg ctaccggaac acacgctgag       60

gtgcagctgg tggagagcgg cggaggccct gtgcagccag gaggctctct gaagctgagc      120

tgcgctgctc cggcttcaca ttttctaact acgagatgta ctgggtgcgc caggctccag      180

gaaagggact ggagtgggtg agctacatct ctacaagccc cgctatcacc tactacgccg      240

actctgtgag gggcaggttc ccatcagcag ggataacgcc aagagctccc tgtacctgca      300

catgaacagc ctgagagctg aggacacagc cgtgtactac tgcgctacca tctcccacca      360

gcagttctct agcggctgga acgcctggtt tgatcctggg gacagggcac actggtgacc      420

gtgtcctcta gctccgagtc cgctaggaac cccacaatct accctctgac cctgccccct      480

gccctgtcta gcgaccccgt gatcatcgga tgtctgatcc acgattactt ccttccggca      540

caatgaacgt gacctgggga aagtctggca aggacatcac cacagtgaac tttccacccg      600

ctctggccag cggaggcaga tacacaatgt cctctcagct gaccctgcca gctgtggagt      660

gccctgagga gagtctgtga agtgtagcgt gcagcacgac tctaaccctg tgcaggagct      720

ggatgtgaac tgcagcggcc caacacctcc accccctatc accatcccat cctgtcagcc      780

ctccctgtct ctgcagaggc cagtctggag gacctgctgc tgggctctga tgccagcatc      840

acctgcacac tgaacggact gcgcaaccca gagggagccg tgttcacctg ggagccctct      900

accggaaagg atgctgtgca gaagaaggcc gtgcagaacc ctgcggctgt tactctgtga      960

gctccgtgct gccaggatgc gctgagcggt ggaactccgg agcttctttc aagtgtaccg     1020

tgacacaccc cgagtccgga accctgacag gcaccatcgc caaggtgaca gtaacacctt     1080

tccaccccag gtgcacctgc tgcctccacc cagcgaggag ctggctctga acgagctgct     1140

gtccctgaca tgtctggtgc gggccttcaa ccccaaggag gtgctggtgc gctggctgca     1200

cggcaacagg agctgagccc tgagtcctac ctggtgtttg agcctctgaa ggagccagga     1260

gagggcgcta ccacatacct ggtgacaagc gtgctgaggg tgtccgccga gacctggaag     1320

cagggagatc agtacagctg ctggtgggac acgaggccct gcctatgaac tttacacaga     1380

agaccatcga cagactgtcc ggcaagccaa ccaacgtgag cgtgtccgtg atcatgtctg     1440

agggagatgg catctgttac tgataa                                          1466


<210>  10
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb41_LC_WT: AA

<400>  10

Met Ala Trp Thr Pro Leu Leu Leu Pro Leu Leu Thr Phe Cys Thr Val 
1               5                   10                  15      


Ser Glu Ala Ser Asn Phe Met Leu Thr Gln Pro His Ser Val Ser Glu 
            20                  25                  30          


Ser Pro Gly Lys Thr Val Thr Ile Ser Cys Thr Gly Ser Ser Gly Ser 
        35                  40                  45              


Ile Ala Ser Asn Tyr Val Gln Trp Tyr Arg Gln Arg Pro Gly Ser Ala 
    50                  55                  60                  


Pro Thr Thr Val Ile Tyr Glu Asn Tyr Gln Arg Pro Ser Gly Val Pro 
65                  70                  75                  80  


Ala Arg Phe Ser Gly Ser Ile Asp Arg Ser Ser Asn Ser Ala Ser Leu 
                85                  90                  95      


Thr Ile Ser Gly Leu Gln Thr Asp Asp Glu Ala Asp Tyr Tyr Cys Gln 
            100                 105                 110         


Ser Tyr Asp Asn Asn Asn Leu Trp Val Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Thr Val Leu Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ser Ser 
    130                 135                 140                 


Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Glu Thr Asn 
145                 150                 155                 160 


Lys Ala Thr Leu Val Cys Thr Ile Thr Asp Phe Tyr Pro Gly Val Val 
                165                 170                 175     


Thr Val Asp Trp Lys Val Asp Gly Thr Pro Val Thr Gln Gly Met Glu 
            180                 185                 190         


Thr Thr Gln Pro Ser Lys Gln Ser Asn Asn Lys Tyr Met Ala Ser Ser 
        195                 200                 205             


Tyr Leu Thr Leu Thr Ala Arg Ala Trp Glu Arg His Ser Ser Tyr Ser 
    210                 215                 220                 


Cys Gln Val Thr His Glu Gly His Thr Val Glu Lys Ser Leu Ser Arg 
225                 230                 235                 240 


Ala Asp Cys Ser 
                


<210>  11
<211>  733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mAb41_LC_WT: Nucleic Acid

<400>  11
atggcttgga ccccactgct gctgcccctg ctgaccttct gcacagtgag cgaggcctcc       60

aactttatgc tgacccagcc tcacagcgtg tccgagtctc caggaaagac cgtgacaatc      120

tcctgtaagg aagctccggc tctatcgcta gcaactacgt gcagtggtac aggcagagac      180

ctggcagcgc cccaaccaca gtgatctacg agaactacca gaggccctcc ggagtgcctg      240

ccagattcag cggctccatc accggtctag caactctgct agcctgacca tctctggcct      300

gcagacagac gatgaggccg actactactg ccagagctac gataacaaca acctgtgggt      360

gtttggcgga ggcaccaagc tgacagtgct ggaacaaaac tgacagtgct gggccagcca      420

aagtcctctc ccagcgtgac cctgttccct cccagcagcg aggagctgga gaccaacaag      480

gccaccctgg tgtgcaccat cacagacttt tacccaggag tggtacagtg gactggaagg      540

tggatggaac ccccgtgaca cagggcatgg agaccacaca gccttccaag cagtctaaca      600

acaagtacat ggcttctagc tacctgaccc tgacagcccg ggcttgggag aggcatcctc      660

ttactcctgc caggtgaccc acgagggcca cacagtggag aagtccctgt ctagagccga      720

ttgttcttga taa                                                         733


<210>  12
<211>  490
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb41_HC_MOD1: AA

<400>  12

Met Asp Trp Thr Trp Arg Ile Leu Phe Leu Val Ala Ala Ala Thr Gly 
1               5                   10                  15      


Thr His Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe 
        35                  40                  45              


Ser Asn Tyr Glu Met Tyr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu 
    50                  55                  60                  


Glu Trp Val Ser Tyr Ile Ser Thr Ser Pro Ala Ile Thr Tyr Tyr Ala 
65                  70                  75                  80  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                85                  90                  95      


Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Thr Ile Ser His Gln Gln Phe Ser Ser Gly Trp Asn 
        115                 120                 125             


Ala Trp Phe Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
    130                 135                 140                 


Ser Ser Glu Ser Ala Arg Asn Pro Thr Ile Tyr Pro Leu Thr Leu Pro 
145                 150                 155                 160 


Pro Ala Leu Ser Ser Asp Pro Val Ile Ile Gly Cys Leu Ile His Asp 
                165                 170                 175     


Tyr Phe Pro Ser Gly Thr Met Asn Val Thr Trp Gly Lys Ser Gly Lys 
            180                 185                 190         


Asp Ile Thr Thr Val Asn Phe Pro Pro Ala Leu Ala Ser Gly Gly Arg 
        195                 200                 205             


Tyr Thr Met Ser Ser Gln Leu Thr Leu Pro Ala Val Glu Cys Pro Glu 
    210                 215                 220                 


Gly Glu Ser Val Lys Cys Ser Val Gln His Asp Ser Asn Pro Val Gln 
225                 230                 235                 240 


Glu Leu Asp Val Asn Cys Ser Gly Pro Thr Pro Pro Pro Pro Ile Thr 
                245                 250                 255     


Ile Pro Ser Cys Gln Pro Ser Leu Ser Leu Gln Arg Pro Ala Leu Glu 
            260                 265                 270         


Asp Leu Leu Leu Gly Ser Asp Ala Ser Ile Thr Cys Thr Leu Asn Gly 
        275                 280                 285             


Leu Arg Asn Pro Glu Gly Ala Val Phe Thr Trp Glu Pro Ser Thr Gly 
    290                 295                 300                 


Lys Asp Ala Val Gln Lys Lys Ala Val Gln Asn Ser Cys Gly Cys Tyr 
305                 310                 315                 320 


Ser Val Ser Ser Val Leu Pro Gly Cys Ala Glu Arg Trp Asn Ser Gly 
                325                 330                 335     


Ala Ser Phe Lys Cys Thr Val Thr His Pro Glu Ser Gly Thr Leu Thr 
            340                 345                 350         


Gly Thr Ile Ala Lys Val Thr Val Asn Thr Phe Pro Pro Gln Val His 
        355                 360                 365             


Leu Leu Pro Pro Pro Ser Glu Glu Leu Ala Leu Asn Glu Leu Leu Ser 
    370                 375                 380                 


Leu Thr Cys Leu Val Arg Ala Phe Asn Pro Lys Glu Val Leu Val Arg 
385                 390                 395                 400 


Trp Leu His Gly Asn Glu Glu Leu Ser Pro Glu Ser Tyr Leu Val Phe 
                405                 410                 415     


Glu Pro Leu Lys Glu Pro Gly Glu Gly Ala Thr Thr Tyr Leu Val Thr 
            420                 425                 430         


Ser Val Leu Arg Val Ser Ala Glu Thr Trp Lys Gln Gly Asp Gln Tyr 
        435                 440                 445             


Ser Cys Met Val Gly His Glu Ala Leu Pro Met Asn Phe Thr Gln Lys 
    450                 455                 460                 


Thr Ile Asp Arg Leu Ser Gly Lys Pro Thr Asn Val Ser Val Ser Val 
465                 470                 475                 480 


Ile Met Ser Glu Gly Asp Gly Ile Cys Tyr 
                485                 490 


<210>  13
<211>  1466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mAb41_HC_MOD1: Nucleic Acid

<400>  13
atggactgga cctggagaat cctgttcctg gtggctgctg ctaccggaac acacgctgag       60

gtgcagctgg tggagagcgg cggaggcctg gtgcagccag gaggctctct gaggctgagc      120

tgcgctgctc cggcttcaca ttttctaact acgagatgta ctgggtgcgc caggctccag      180

gaaagggact ggagtgggtg agctacatct ctacaagccc cgctatcacc tactacgccg      240

actctgtgaa gggcaggttc ccatcagcag ggataacgcc aagaactccc tgtacctgca      300

gatgaacagc ctgagagctg aggacacagc cgtgtactac tgcgctacca tctcccacca      360

gcagttctct agcggctgga acgcctggtt tgatcctggg gacagggcac actggtgacc      420

gtgtcctcta gctccgagtc cgctaggaac cccacaatct accctctgac cctgccccct      480

gccctgtcta gcgaccccgt gatcatcgga tgtctgatcc acgattactt ccttccggca      540

caatgaacgt gacctgggga aagtctggca aggacatcac cacagtgaac tttccacccg      600

ctctggccag cggaggcaga tacacaatgt cctctcagct gaccctgcca gctgtggagt      660

gccctgaggg aagtctgtga agtgtagcgt gcagcacgac tctaaccctg tgcaggagct      720

ggatgtgaac tgcagcggcc caacacctcc accccctatc accatcccat cctgtcagcc      780

ctccctgtct ctgcagaggc cagcttggag gacctgctgc tgggctctga tgccagcatc      840

acctgcacac tgaacggact gcgcaaccca gagggagccg tgttcacctg ggagccctct      900

accggaaagg atgctgtgca gaagaaggcc gtgcagaact ccgcggctgt tactctgtga      960

gctccgtgct gccaggatgc gctgagcggt ggaactccgg agcttctttc aagtgtaccg     1020

tgacacaccc cgagtccgga accctgacag gcaccatcgc caaggtgaca gtgaaccctt     1080

tccaccccag gtgcacctgc tgcctccacc cagcgaggag ctggctctga acgagctgct     1140

gtccctgaca tgtctggtgc gggccttcaa ccccaaggag gtgctggtgc gctggctgca     1200

cggcaacgag gactgagccc tgagtcctac ctggtgtttg agcctctgaa ggagccagga     1260

gagggcgcta ccacatacct ggtgacaagc gtgctgaggg tgtccgccga gacctggaag     1320

cagggagatc agtacagctg catggtggac acgaggccct gcctatgaac tttacacaga     1380

agaccatcga cagactgtcc ggcaagccaa ccaacgtgag cgtgtccgtg atcatgtctg     1440

agggagatgg catctgttac tgataa                                          1466


<210>  14
<211>  244
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mAb41_LC_MOD1: AA

<400>  14

Met Ala Trp Thr Pro Leu Leu Leu Pro Leu Leu Thr Phe Cys Thr Val 
1               5                   10                  15      


Ser Glu Ala Ser Asn Phe Met Leu Thr Gln Pro His Ser Val Ser Glu 
            20                  25                  30          


Ser Pro Gly Lys Thr Val Thr Ile Ser Cys Thr Gly Ser Ser Gly Ser 
        35                  40                  45              


Ile Ala Ser Asn Tyr Val Gln Trp Tyr Gln Gln Arg Pro Gly Ser Ser 
    50                  55                  60                  


Pro Thr Thr Val Ile Tyr Glu Asn Tyr Gln Arg Pro Ser Gly Val Pro 
65                  70                  75                  80  


Asp Arg Phe Ser Gly Ser Ile Asp Ser Ser Ser Asn Ser Ala Ser Leu 
                85                  90                  95      


Thr Ile Ser Gly Leu Lys Thr Glu Asp Glu Ala Asp Tyr Tyr Cys Gln 
            100                 105                 110         


Ser Tyr Asp Asn Asn Asn Leu Trp Val Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Thr Val Leu Gly Thr Lys Leu Thr Val Leu Gly Gln Pro Lys Ser Ser 
    130                 135                 140                 


Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Glu Thr Asn 
145                 150                 155                 160 


Lys Ala Thr Leu Val Cys Thr Ile Thr Asp Phe Tyr Pro Gly Val Val 
                165                 170                 175     


Thr Val Asp Trp Lys Val Asp Gly Thr Pro Val Thr Gln Gly Met Glu 
            180                 185                 190         


Thr Thr Gln Pro Ser Lys Gln Ser Asn Asn Lys Tyr Met Ala Ser Ser 
        195                 200                 205             


Tyr Leu Thr Leu Thr Ala Arg Ala Trp Glu Arg His Ser Ser Tyr Ser 
    210                 215                 220                 


Cys Gln Val Thr His Glu Gly His Thr Val Glu Lys Ser Leu Ser Arg 
225                 230                 235                 240 


Ala Asp Cys Ser 
                


<210>  15
<211>  733
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mAb41_LC_MOD1: Nucleic Acid

<400>  15
atggcttgga ccccactgct gctgcccctg ctgaccttct gcacagtgag cgaggcctcc       60

aactttatgc tgacccagcc tcacagcgtg tccgagtctc caggaaagac cgtgacaatc      120

tcctgtaagg aagctccggc tctatcgcta gcaactacgt gcagtggtac cagcagagac      180

ctggcagcag cccaaccaca gtgatctacg agaactacca gaggccctcc ggagtgcctg      240

acagattcag cggctccatc gacactctag caactctgct agcctgacca tctctggcct      300

gaagacagag gatgaggccg actactactg ccagagctac gataacaaca acctgtgggt      360

gtttggcgga ggcaccaagc tgacagtgct gggaacaaaa tgacagtgct gggccagcca      420

aagtcctctc ccagcgtgac cctgttccct cccagcagcg aggagctgga gaccaacaag      480

gccaccctgg tgtgcaccat cacagacttt tacccaggag tggtgacagt ggactgaagg      540

tggatggaac ccccgtgaca cagggcatgg agaccacaca gccttccaag cagtctaaca      600

acaagtacat ggcttctagc tacctgaccc tgacagcccg ggcttgggag aggcactcct      660

cttactcctg caggtgaccc acgagggcca cacagtggag aagtccctgt ctagagccga      720

ttgttcttga taa                                                         733


<210>  16
<211>  159
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse J Chain: AA

<400>  16

Met Lys Thr His Leu Leu Leu Trp Gly Val Leu Ala Ile Phe Val Lys 
1               5                   10                  15      


Ala Val Leu Val Thr Gly Asp Asp Glu Ala Thr Ile Leu Ala Asp Asn 
            20                  25                  30          


Lys Cys Met Cys Thr Arg Val Thr Ser Arg Ile Ile Pro Ser Thr Glu 
        35                  40                  45              


Asp Pro Asn Glu Asp Ile Val Glu Arg Asn Ile Arg Ile Val Val Pro 
    50                  55                  60                  


Leu Asn Asn Arg Glu Asn Ile Ser Asp Pro Thr Ser Pro Leu Arg Arg 
65                  70                  75                  80  


Asn Phe Val Tyr His Leu Ser Asp Val Cys Lys Lys Cys Asp Pro Val 
                85                  90                  95      


Glu Val Glu Leu Glu Asp Gln Val Val Thr Ala Thr Gln Ser Asn Ile 
            100                 105                 110         


Cys Asn Glu Asp Asp Gly Val Pro Glu Thr Cys Tyr Met Tyr Asp Arg 
        115                 120                 125             


Asn Lys Cys Tyr Thr Thr Met Val Pro Leu Arg Tyr His Gly Glu Thr 
    130                 135                 140                 


Lys Met Val Gln Ala Ala Leu Thr Pro Asp Ser Cys Tyr Pro Asp 
145                 150                 155                 


<210>  17
<211>  477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mouse J Chain: Nucleic Acid

<400>  17
atgaaaactc acctcctcct gtggggtgtg ctggctatct ttgtcaaggc tgtgctcgtc       60

actggggatg atgaagctac tattctcgcc gacaacaagt gcatgtgcac cagggtgaca      120

tctagaatca tcccaagcac cgaggacccc aacgaggata tcgtggagcg gaacatccgc      180

atcgtggtgc ccctgaacaa ccgggagaac atcagcgatc ccacatcccc tctgaggaga      240

aacttcgtgt accacctgtc cgacgtgtgc aagaagtgtg atcctgtgga ggtggagctg      300

gaggaccagg tggtgaccgc tacacagtct aacatctgca acgaggacga tggcgtgcct      360

gagacatgct acatgtacga tcggaacaag tgttacacca caatggtgcc actgcgctat      420

cacggcgaga ctaaaatggt ccaggctgct ctgactcccg acagttgtta tcccgac         477


