                         SEQUENCE LISTING

<110>  Horizon Discovery Limited
 
<120>  RNA scaffolds

<130>  P33290WO1

<160>  186   

<170>  PatentIn version 3.5

<210>  1
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wild type Cas9 protein

<400>  1

Met Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  2
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Uracil-DNA glycosylase inhibitor (UGI)

<400>  2

Met Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu 
1               5                   10                  15      


Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val 
            20                  25                  30          


Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp 
        35                  40                  45              


Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu 
    50                  55                  60                  


Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys 
65                  70                  75                  80  


Ile Lys Met Leu 
                


<210>  3
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  exemplary hybrid crRNA:tracrRNA, gRNA sequence

<400>  3
guuuaagagc uaugcuggaa acagcauagc aaguuuaaau aaggcuaguc cguuaucaac       60

uugaaaaagu ggcaccgagu cggugcuuuu uuu                                    93


<210>  4
<211>  79
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 1

<400>  4
ggaaccauuc aaaacagcau agcaaguuaa aauaaggcua guccguuauc aacuugaaaa       60

aguggcaccg agucggugc                                                    79


<210>  5
<211>  60
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 2

<400>  5
uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc gagucggugc       60


<210>  6
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 3

<400>  6
agcauagcaa guuaaaauaa ggcuaguccg uuaucaacuu gaaaaagugg caccgagucg       60

gugc                                                                    64


<210>  7
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 4

<400>  7
caaaacagca uagcaaguua aaauaaggcu aguccguuau caacuugaaa aaguggcacc       60

gagucggugc                                                              70


<210>  8
<211>  45
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 5

<400>  8
uagcaaguua aaauaaggcu aguccguuau caacuugaaa aagug                       45


<210>  9
<211>  32
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 6

<400>  9
uagcaaguua aaauaaggcu aguccguuau ca                                     32


<210>  10
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA 7

<400>  10
uagcaaguua aaauaaggcu aguccg                                            26


<210>  11
<211>  66
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Telomerase Ku Binding Motif

<400>  11
uucuugucgu acuuauagau cgcuacguua uuucaauuuu gaaaaucuga guccugggag       60

ugcgga                                                                  66


<210>  12
<211>  1094
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Telomerase Ku Heterodimer

<400>  12

Met Ser Gly Trp Glu Ser Tyr Tyr Lys Thr Glu Gly Asp Glu Glu Ala 
1               5                   10                  15      


Glu Glu Glu Gln Glu Glu Asn Leu Glu Ala Ser Gly Asp Tyr Lys Tyr 
            20                  25                  30          


Ser Gly Arg Asp Ser Leu Ile Phe Leu Val Asp Ala Ser Lys Ala Met 
        35                  40                  45              


Phe Glu Ser Gln Ser Glu Asp Glu Leu Thr Pro Phe Asp Met Ser Ile 
    50                  55                  60                  


Gln Cys Ile Gln Ser Val Tyr Ile Ser Lys Ile Ile Ser Ser Asp Arg 
65                  70                  75                  80  


Asp Leu Leu Ala Val Val Phe Tyr Gly Thr Glu Lys Asp Lys Asn Ser 
                85                  90                  95      


Val Asn Phe Lys Asn Ile Tyr Val Leu Gln Glu Leu Asp Asn Pro Gly 
            100                 105                 110         


Ala Lys Arg Ile Leu Glu Leu Asp Gln Phe Lys Gly Gln Gln Gly Gln 
        115                 120                 125             


Lys Arg Phe Gln Asp Met Met Gly His Gly Ser Asp Tyr Ser Leu Ser 
    130                 135                 140                 


Glu Val Leu Trp Val Cys Ala Asn Leu Phe Ser Asp Val Gln Phe Lys 
145                 150                 155                 160 


Met Ser His Lys Arg Ile Met Leu Phe Thr Asn Glu Asp Asn Pro His 
                165                 170                 175     


Gly Asn Asp Ser Ala Lys Ala Ser Arg Ala Arg Thr Lys Ala Gly Asp 
            180                 185                 190         


Leu Arg Asp Thr Gly Ile Phe Leu Asp Leu Met His Leu Lys Lys Pro 
        195                 200                 205             


Gly Gly Phe Asp Ile Ser Leu Phe Tyr Arg Asp Ile Ile Ser Ile Ala 
    210                 215                 220                 


Glu Asp Glu Asp Leu Arg Val His Phe Glu Glu Ser Ser Lys Leu Glu 
225                 230                 235                 240 


Asp Leu Leu Arg Lys Val Arg Ala Lys Glu Thr Arg Lys Arg Ala Leu 
                245                 250                 255     


Ser Arg Leu Lys Leu Lys Leu Asn Lys Asp Ile Val Ile Ser Val Gly 
            260                 265                 270         


Ile Tyr Asn Leu Val Gln Lys Ala Leu Lys Pro Pro Pro Ile Lys Leu 
        275                 280                 285             


Tyr Arg Glu Thr Asn Glu Pro Val Lys Thr Lys Thr Arg Thr Phe Asn 
    290                 295                 300                 


Thr Ser Thr Gly Gly Leu Leu Leu Pro Ser Asp Thr Lys Arg Ser Gln 
305                 310                 315                 320 


Ile Tyr Gly Ser Arg Gln Ile Ile Leu Glu Lys Glu Glu Thr Glu Glu 
                325                 330                 335     


Leu Lys Arg Phe Asp Asp Pro Gly Leu Met Leu Met Gly Phe Lys Pro 
            340                 345                 350         


Leu Val Leu Leu Lys Lys His His Tyr Leu Arg Pro Ser Leu Phe Val 
        355                 360                 365             


Tyr Pro Glu Glu Ser Leu Val Ile Gly Ser Ser Thr Leu Phe Ser Ala 
    370                 375                 380                 


Leu Leu Ile Lys Cys Leu Glu Lys Glu Val Ala Ala Leu Cys Arg Tyr 
385                 390                 395                 400 


Thr Pro Arg Arg Asn Ile Pro Pro Tyr Phe Val Ala Leu Val Pro Gln 
                405                 410                 415     


Glu Glu Glu Leu Asp Asp Gln Lys Ile Gln Val Thr Pro Pro Gly Phe 
            420                 425                 430         


Gln Leu Val Phe Leu Pro Phe Ala Asp Asp Lys Arg Lys Met Pro Phe 
        435                 440                 445             


Thr Glu Lys Ile Met Ala Thr Pro Glu Gln Val Gly Lys Met Lys Ala 
    450                 455                 460                 


Ile Val Glu Lys Leu Arg Phe Thr Tyr Arg Ser Asp Ser Phe Glu Asn 
465                 470                 475                 480 


Pro Val Leu Gln Gln His Phe Arg Asn Leu Glu Ala Leu Ala Leu Asp 
                485                 490                 495     


Leu Met Glu Pro Glu Gln Ala Val Asp Leu Thr Leu Pro Lys Val Glu 
            500                 505                 510         


Ala Met Asn Lys Arg Leu Gly Ser Leu Val Asp Glu Phe Lys Glu Leu 
        515                 520                 525             


Val Tyr Pro Pro Asp Tyr Asn Pro Glu Gly Lys Val Thr Lys Arg Lys 
    530                 535                 540                 


His Asp Asn Glu Gly Ser Gly Ser Lys Arg Pro Lys Val Glu Tyr Ser 
545                 550                 555                 560 


Glu Glu Glu Leu Lys Thr His Ile Ser Lys Gly Thr Leu Gly Lys Phe 
                565                 570                 575     


Thr Val Pro Met Leu Lys Glu Ala Cys Arg Ala Tyr Gly Leu Lys Ser 
            580                 585                 590         


Gly Leu Lys Lys Gln Glu Leu Leu Glu Ala Leu Thr Lys His Phe Gln 
        595                 600                 605             


Asp Met Val Arg Ser Gly Asn Lys Ala Ala Val Val Leu Cys Met Asp 
    610                 615                 620                 


Val Gly Phe Thr Met Ser Asn Ser Ile Pro Gly Ile Glu Ser Pro Phe 
625                 630                 635                 640 


Glu Gln Ala Lys Lys Val Ile Thr Met Phe Val Gln Arg Gln Val Phe 
                645                 650                 655     


Ala Glu Asn Lys Asp Glu Ile Ala Leu Val Leu Phe Gly Thr Asp Gly 
            660                 665                 670         


Thr Asp Asn Pro Leu Ser Gly Gly Asp Gln Tyr Gln Asn Ile Thr Val 
        675                 680                 685             


His Arg His Leu Met Leu Pro Asp Phe Asp Leu Leu Glu Asp Ile Glu 
    690                 695                 700                 


Ser Lys Ile Gln Pro Gly Ser Gln Gln Ala Asp Phe Leu Asp Ala Leu 
705                 710                 715                 720 


Ile Val Ser Met Asp Val Ile Gln His Glu Thr Ile Gly Lys Lys Phe 
                725                 730                 735     


Glu Lys Arg His Ile Glu Ile Phe Thr Asp Leu Ser Ser Arg Phe Ser 
            740                 745                 750         


Lys Ser Gln Leu Asp Ile Ile Ile His Ser Leu Lys Lys Cys Asp Ile 
        755                 760                 765             


Ser Glu Arg His Ser Ile His Trp Pro Cys Arg Leu Thr Ile Gly Ser 
    770                 775                 780                 


Asn Leu Ser Ile Arg Ile Ala Ala Tyr Lys Ser Ile Leu Gln Glu Arg 
785                 790                 795                 800 


Val Lys Lys Thr Trp Thr Val Val Asp Ala Lys Thr Leu Lys Lys Glu 
                805                 810                 815     


Asp Ile Gln Lys Glu Thr Val Tyr Cys Leu Asn Asp Asp Asp Glu Thr 
            820                 825                 830         


Glu Val Leu Lys Glu Asp Ile Ile Gln Gly Phe Arg Tyr Gly Ser Asp 
        835                 840                 845             


Ile Val Pro Phe Ser Lys Val Asp Glu Glu Gln Met Lys Tyr Lys Ser 
    850                 855                 860                 


Glu Gly Lys Cys Phe Ser Val Leu Gly Phe Cys Lys Ser Ser Gln Val 
865                 870                 875                 880 


Gln Arg Arg Phe Phe Met Gly Asn Gln Val Leu Lys Val Phe Ala Ala 
                885                 890                 895     


Arg Asp Asp Glu Ala Ala Ala Val Ala Leu Ser Ser Leu Ile His Ala 
            900                 905                 910         


Leu Asp Asp Leu Asp Met Val Ala Ile Val Arg Tyr Ala Tyr Asp Lys 
        915                 920                 925             


Arg Ala Asn Pro Gln Val Gly Val Ala Phe Pro His Ile Lys His Asn 
    930                 935                 940                 


Tyr Glu Cys Leu Val Tyr Val Gln Leu Pro Phe Met Glu Asp Leu Arg 
945                 950                 955                 960 


Gln Tyr Met Phe Ser Ser Leu Lys Asn Ser Lys Lys Tyr Ala Pro Thr 
                965                 970                 975     


Glu Ala Gln Leu Asn Ala Val Asp Ala Leu Ile Asp Ser Met Ser Leu 
            980                 985                 990         


Ala Lys Lys Asp Glu Lys Thr Asp  Thr Leu Glu Asp Leu  Phe Pro Thr 
        995                 1000                 1005             


Thr Lys  Ile Pro Asn Pro Arg  Phe Gln Arg Leu Phe  Gln Cys Leu 
    1010                 1015                 1020             


Leu His  Arg Ala Leu His Pro  Arg Glu Pro Leu Pro  Pro Ile Gln 
    1025                 1030                 1035             


Gln His  Ile Trp Asn Met Leu  Asn Pro Pro Ala Glu  Val Thr Thr 
    1040                 1045                 1050             


Lys Ser  Gln Ile Pro Leu Ser  Lys Ile Lys Thr Leu  Phe Pro Leu 
    1055                 1060                 1065             


Ile Glu  Ala Lys Lys Lys Asp  Gln Val Thr Ala Gln  Glu Ile Phe 
    1070                 1075                 1080             


Gln Asp  Asn His Glu Asp Gly  Pro Thr Ala Lys 
    1085                 1090                 


<210>  13
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Telomerase Sm7 consensus site (single stranded)

<400>  13
aauuuuugga                                                              10


<210>  14
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Monomeric Sm-like protein (archaea)

<400>  14

Gly Ser Val Ile Asp Val Ser Ser Gln Arg Val Asn Val Gln Arg Pro 
1               5                   10                  15      


Leu Asp Ala Leu Gly Asn Ser Leu Asn Ser Pro Val Ile Ile Lys Leu 
            20                  25                  30          


Lys Gly Asp Arg Glu Phe Arg Gly Val Leu Lys Ser Phe Asp Leu His 
        35                  40                  45              


Met Asn Leu Val Leu Asn Asp Ala Glu Glu Leu Glu Asp Gly Glu Val 
    50                  55                  60                  


Thr Arg Arg Leu Gly Thr Val Leu Ile Arg Gly Asp Asn Ile Val Tyr 
65                  70                  75                  80  


Ile Ser Pro 
            


<210>  15
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MS2 phage operator stem loop

<400>  15
acaugaggau cacccaugu                                                    19


<210>  16
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MS2 coat protein

<400>  16

Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr 
1               5                   10                  15      


Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Ile Ala Glu 
            20                  25                  30          


Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser 
        35                  40                  45              


Val Arg Gln Ser Ser Ala Gln Asn Arg Lys Tyr Thr Ile Lys Val Glu 
    50                  55                  60                  


Val Pro Lys Gly Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile 
65                  70                  75                  80  


Pro Ile Phe Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met 
                85                  90                  95      


Gln Gly Leu Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala 
            100                 105                 110         


Asn Ser Gly Ile Tyr 
        115         


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PP7 phage operator stem loop

<400>  17
auaaggaguu uauauggaaa cccuua                                            26


<210>  18
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PP7 coat protein (PCP)

<400>  18

Met Ser Lys Thr Ile Val Leu Ser Val Gly Glu Ala Thr Arg Thr Leu 
1               5                   10                  15      


Thr Glu Ile Gln Ser Thr Ala Asp Arg Gln Ile Phe Glu Glu Lys Val 
            20                  25                  30          


Gly Pro Leu Val Gly Arg Leu Arg Leu Thr Ala Ser Leu Arg Gln Asn 
        35                  40                  45              


Gly Ala Lys Thr Ala Tyr Arg Val Asn Leu Lys Leu Asp Gln Ala Asp 
    50                  55                  60                  


Val Val Asp Cys Ser Thr Ser Val Cys Gly Glu Leu Pro Lys Val Arg 
65                  70                  75                  80  


Tyr Thr Gln Val Trp Ser His Asp Val Thr Ile Val Ala Asn Ser Thr 
                85                  90                  95      


Glu Ala Ser Arg Lys Ser Leu Tyr Asp Leu Thr Lys Ser Leu Val Ala 
            100                 105                 110         


Thr Ser Gln Val Glu Asp Leu Val Val Asn Leu Val Pro Leu Gly Arg 
        115                 120                 125             


<210>  19
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SfMu Com stem loop

<400>  19
cugaaugccu gcgagcauc                                                    19


<210>  20
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SfMu Com binding protein

<400>  20

Met Lys Ser Ile Arg Cys Lys Asn Cys Asn Lys Leu Leu Phe Lys Ala 
1               5                   10                  15      


Asp Ser Phe Asp His Ile Glu Ile Arg Cys Pro Arg Cys Lys Arg His 
            20                  25                  30          


Ile Ile Met Leu Asn Ala Cys Glu His Pro Thr Glu Lys His Cys Gly 
        35                  40                  45              


Lys Arg Glu Lys Ile Thr His Ser Asp Glu Thr Val Arg Tyr 
    50                  55                  60          


<210>  21
<211>  25
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  4nt MS2 extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  GC linker

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Extension

<220>
<221>  misc_feature
<222>  (4)..(23)
<223>  MS2 aptamer

<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  Extension

<400>  21
gcgcacauga ggaucaccca ugugc                                             25


<210>  22
<211>  31
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  10nt MS2 extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linker

<220>
<221>  misc_feature
<222>  (3)..(7)
<223>  Extension

<220>
<221>  misc_feature
<222>  (8)..(26)
<223>  MS2 Aptamer

<220>
<221>  misc_feature
<222>  (27)..(31)
<223>  Extension

<400>  22
gcgagcgaca ugaggaucac ccaugucgcu c                                      31


<210>  23
<211>  37
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  16 nt MS2 extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linker

<220>
<221>  misc_feature
<222>  (3)..(10)
<223>  Extension

<220>
<221>  misc_feature
<222>  (11)..(29)
<223>  MS2 Aptamer

<220>
<221>  misc_feature
<222>  (30)..(37)
<223>  Extension

<400>  23
gccacgagcg acaugaggau cacccauguc gcucgug                                37


<210>  24
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  26nt MS2 extension


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Linker

<220>
<221>  misc_feature
<222>  (3)..(15)
<223>  Linker

<220>
<221>  misc_feature
<222>  (16)..(34)
<223>  MS2 aptamer

<220>
<221>  misc_feature
<222>  (35)..(47)
<223>  Extension

<400>  24
gccgucagac gagcgacaug aggaucaccc augucgcucg ucugacg                     47


<210>  25
<211>  1368
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S. pyogenes dCas9 protein sequence


<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Active site mutant D10A

<220>
<221>  MISC_FEATURE
<222>  (840)..(840)
<223>  Active site mutant H840A

<400>  25

Met Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
1               5                   10                  15      


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            20                  25                  30          


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        35                  40                  45              


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    50                  55                  60                  


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
65                  70                  75                  80  


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                85                  90                  95      


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            100                 105                 110         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        115                 120                 125             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    130                 135                 140                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
145                 150                 155                 160 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                165                 170                 175     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            180                 185                 190         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        195                 200                 205             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    210                 215                 220                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
225                 230                 235                 240 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                245                 250                 255     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            260                 265                 270         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        275                 280                 285             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    290                 295                 300                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
305                 310                 315                 320 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                325                 330                 335     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            340                 345                 350         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        355                 360                 365             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    370                 375                 380                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
385                 390                 395                 400 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                405                 410                 415     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            420                 425                 430         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        435                 440                 445             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    450                 455                 460                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
465                 470                 475                 480 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                485                 490                 495     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            500                 505                 510         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        515                 520                 525             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    530                 535                 540                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
545                 550                 555                 560 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                565                 570                 575     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            580                 585                 590         


Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp 
        595                 600                 605             


Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr 
    610                 615                 620                 


Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala 
625                 630                 635                 640 


His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr 
                645                 650                 655     


Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp 
            660                 665                 670         


Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe 
        675                 680                 685             


Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe 
    690                 695                 700                 


Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu 
705                 710                 715                 720 


His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly 
                725                 730                 735     


Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly 
            740                 745                 750         


Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln 
        755                 760                 765             


Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile 
    770                 775                 780                 


Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro 
785                 790                 795                 800 


Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu 
                805                 810                 815     


Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg 
            820                 825                 830         


Leu Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys 
        835                 840                 845             


Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg 
    850                 855                 860                 


Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys 
865                 870                 875                 880 


Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys 
                885                 890                 895     


Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp 
            900                 905                 910         


Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr 
        915                 920                 925             


Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp 
    930                 935                 940                 


Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser 
945                 950                 955                 960 


Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg 
                965                 970                 975     


Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val 
            980                 985                 990         


Val Gly Thr Ala Leu Ile Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe 
        995                 1000                 1005             


Val Tyr  Gly Asp Tyr Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala 
    1010                 1015                 1020             


Lys Ser  Glu Gln Glu Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe 
    1025                 1030                 1035             


Tyr Ser  Asn Ile Met Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala 
    1040                 1045                 1050             


Asn Gly  Glu Ile Arg Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu 
    1055                 1060                 1065             


Thr Gly  Glu Ile Val Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val 
    1070                 1075                 1080             


Arg Lys  Val Leu Ser Met Pro  Gln Val Asn Ile Val  Lys Lys Thr 
    1085                 1090                 1095             


Glu Val  Gln Thr Gly Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys 
    1100                 1105                 1110             


Arg Asn  Ser Asp Lys Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro 
    1115                 1120                 1125             


Lys Lys  Tyr Gly Gly Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val 
    1130                 1135                 1140             


Leu Val  Val Ala Lys Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys 
    1145                 1150                 1155             


Ser Val  Lys Glu Leu Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser 
    1160                 1165                 1170             


Phe Glu  Lys Asn Pro Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys 
    1175                 1180                 1185             


Glu Val  Lys Lys Asp Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu 
    1190                 1195                 1200             


Phe Glu  Leu Glu Asn Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly 
    1205                 1210                 1215             


Glu Leu  Gln Lys Gly Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val 
    1220                 1225                 1230             


Asn Phe  Leu Tyr Leu Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser 
    1235                 1240                 1245             


Pro Glu  Asp Asn Glu Gln Lys  Gln Leu Phe Val Glu  Gln His Lys 
    1250                 1255                 1260             


His Tyr  Leu Asp Glu Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys 
    1265                 1270                 1275             


Arg Val  Ile Leu Ala Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala 
    1280                 1285                 1290             


Tyr Asn  Lys His Arg Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn 
    1295                 1300                 1305             


Ile Ile  His Leu Phe Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala 
    1310                 1315                 1320             


Phe Lys  Tyr Phe Asp Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser 
    1325                 1330                 1335             


Thr Lys  Glu Val Leu Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr 
    1340                 1345                 1350             


Gly Leu  Tyr Glu Thr Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp 
    1355                 1360                 1365             


<210>  26
<211>  1367
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cas9 D10A Protein


<220>
<221>  Mutation
<222>  (10)..(10)
<223>  D10A

<400>  26

Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys 
            20                  25                  30          


Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly 
        35                  40                  45              


Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys 
    50                  55                  60                  


Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr 
65                  70                  75                  80  


Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe 
                85                  90                  95      


Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His 
            100                 105                 110         


Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His 
        115                 120                 125             


Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser 
    130                 135                 140                 


Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 


Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp 
                165                 170                 175     


Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn 
            180                 185                 190         


Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys 
        195                 200                 205             


Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu 
    210                 215                 220                 


Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu 
225                 230                 235                 240 


Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp 
                245                 250                 255     


Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp 
            260                 265                 270         


Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu 
        275                 280                 285             


Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile 
    290                 295                 300                 


Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met 
305                 310                 315                 320 


Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala 
                325                 330                 335     


Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp 
            340                 345                 350         


Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln 
        355                 360                 365             


Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly 
    370                 375                 380                 


Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys 
385                 390                 395                 400 


Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly 
                405                 410                 415     


Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu 
            420                 425                 430         


Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro 
        435                 440                 445             


Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met 
    450                 455                 460                 


Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val 
465                 470                 475                 480 


Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     


Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu 
            500                 505                 510         


Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr 
        515                 520                 525             


Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 


Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 


Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     


Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 


Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 


Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 


Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg 
    1100                 1105                 1110             


Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Val Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser 
    1145                 1150                 1155             


Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe 
    1160                 1165                 1170             


Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu 
    1175                 1180                 1185             


Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1190                 1195                 1200             


Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu 
    1205                 1210                 1215             


Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
    1220                 1225                 1230             


Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro 
    1235                 1240                 1245             


Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His 
    1250                 1255                 1260             


Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg 
    1265                 1270                 1275             


Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr 
    1280                 1285                 1290             


Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile 
    1295                 1300                 1305             


Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr 
    1325                 1330                 1335             


Lys Glu  Val Leu Asp Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln Leu Gly  Gly Asp 
    1355                 1360                 1365         


<210>  27
<211>  4104
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding Cas9 D10A Protein


<220>
<221>  misc_feature
<222>  (29)..()
<223>  A to C

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  A to C

<400>  27
atggataaaa agtattctat tggtttagcc atcggcacta attccgttgg atgggctgtc       60

ataaccgatg aatacaaagt accttcaaag aaatttaagg tgttggggaa cacagaccgt      120

cattcgatta aaaagaatct tatcggtgcc ctcctattcg atagtggcga aacggcagag      180

gcgactcgcc tgaaacgaac cgctcggaga aggtatacac gtcgcaagaa ccgaatatgt      240

tacttacaag aaatttttag caatgagatg gccaaagttg acgattcttt ctttcaccgt      300

ttggaagagt ccttccttgt cgaagaggac aagaaacatg aacggcaccc catctttgga      360

aacatagtag atgaggtggc atatcatgaa aagtacccaa cgatttatca cctcagaaaa      420

aagctagttg actcaactga taaagcggac ctgaggttaa tctacttggc tcttgcccat      480

atgataaagt tccgtgggca ctttctcatt gagggtgatc taaatccgga caactcggat      540

gtcgacaaac tgttcatcca gttagtacaa acctataatc agttgtttga agagaaccct      600

ataaatgcaa gtggcgtgga tgcgaaggct attcttagcg cccgcctctc taaatcccga      660

cggctagaaa acctgatcgc acaattaccc ggagagaaga aaaatgggtt gttcggtaac      720

cttatagcgc tctcactagg cctgacacca aattttaagt cgaacttcga cttagctgaa      780

gatgccaaat tgcagcttag taaggacacg tacgatgacg atctcgacaa tctactggca      840

caaattggag atcagtatgc ggacttattt ttggctgcca aaaaccttag cgatgcaatc      900

ctcctatctg acatactgag agttaatact gagattacca aggcgccgtt atccgcttca      960

atgatcaaaa ggtacgatga acatcaccaa gacttgacac ttctcaaggc cctagtccgt     1020

cagcaactgc ctgagaaata taaggaaata ttctttgatc agtcgaaaaa cgggtacgca     1080

ggttatattg acggcggagc gagtcaagag gaattctaca agtttatcaa acccatatta     1140

gagaagatgg atgggacgga agagttgctt gtaaaactca atcgcgaaga tctactgcga     1200

aagcagcgga ctttcgacaa cggtagcatt ccacatcaaa tccacttagg cgaattgcat     1260

gctatactta gaaggcagga ggatttttat ccgttcctca aagacaatcg tgaaaagatt     1320

gagaaaatcc taacctttcg cataccttac tatgtgggac ccctggcccg agggaactct     1380

cggttcgcat ggatgacaag aaagtccgaa gaaacgatta ctccatggaa ttttgaggaa     1440

gttgtcgata aaggtgcgtc agctcaatcg ttcatcgaga ggatgaccaa ctttgacaag     1500

aatttaccga acgaaaaagt attgcctaag cacagtttac tttacgagta tttcacagtg     1560

tacaatgaac tcacgaaagt taagtatgtc actgagggca tgcgtaaacc cgcctttcta     1620

agcggagaac agaagaaagc aatagtagat ctgttattca agaccaaccg caaagtgaca     1680

gttaagcaat tgaaagagga ctactttaag aaaattgaat gcttcgattc tgtcgagatc     1740

tccggggtag aagatcgatt taatgcgtca cttggtacgt atcatgacct cctaaagata     1800

attaaagata aggacttcct ggataacgaa gagaatgaag atatcttaga agatatagtg     1860

ttgactctta ccctctttga agatcgggaa atgattgagg aaagactaaa aacatacgct     1920

cacctgttcg acgataaggt tatgaaacag ttaaagaggc gtcgctatac gggctgggga     1980

cgattgtcgc ggaaacttat caacgggata agagacaagc aaagtggtaa aactattctc     2040

gattttctaa agagcgacgg cttcgccaat aggaacttta tgcagctgat ccatgatgac     2100

tctttaacct tcaaagagga tatacaaaag gcacaggttt ccggacaagg ggactcattg     2160

cacgaacata ttgcgaatct tgctggttcg ccagccatca aaaagggcat actccagaca     2220

gtcaaagtag tggatgagct agttaaggtc atgggacgtc acaaaccgga aaacattgta     2280

atcgagatgg cacgcgaaaa tcaaacgact cagaaggggc aaaaaaacag tcgagagcgg     2340

atgaagagaa tagaagaggg tattaaagaa ctgggcagcc agatcttaaa ggagcatcct     2400

gtggaaaata cccaattgca gaacgagaaa ctttacctct attacctaca aaatggaagg     2460

gacatgtatg ttgatcagga actggacata aaccgtttat ctgattacga cgtcgatcac     2520

attgtacccc aatccttttt gaaggacgat tcaatcgaca ataaagtgct tacacgctcg     2580

gataagaacc gagggaaaag tgacaatgtt ccaagcgagg aagtcgtaaa gaaaatgaag     2640

aactattggc ggcagctcct aaatgcgaaa ctgataacgc aaagaaagtt cgataactta     2700

actaaagctg agaggggtgg cttgtctgaa cttgacaagg ccggatttat taaacgtcag     2760

ctcgtggaaa cccgccaaat cacaaagcat gttgcacaga tactagattc ccgaatgaat     2820

acgaaatacg acgagaacga taagctgatt cgggaagtca aagtaatcac tttaaagtca     2880

aaattggtgt cggacttcag aaaggatttt caattctata aagttaggga gataaataac     2940

taccaccatg cgcacgacgc ttatcttaat gccgtcgtag ggaccgcact cattaagaaa     3000

tacccgaagc tagaaagtga gtttgtgtat ggtgattaca aagtttatga cgtccgtaag     3060

atgatcgcga aaagcgaaca ggagataggc aaggctacag ccaaatactt cttttattct     3120

aacattatga atttctttaa gacggaaatc actctggcaa acggagagat acgcaaacga     3180

cctttaattg aaaccaatgg ggagacaggt gaaatcgtat gggataaggg ccgggacttc     3240

gcgacggtga gaaaagtttt gtccatgccc caagtcaaca tagtaaagaa aactgaggtg     3300

cagaccggag ggttttcaaa ggaatcgatt cttccaaaaa ggaatagtga taagctcatc     3360

gctcgtaaaa aggactggga cccgaaaaag tacggtggct tcgatagccc tacagttgcc     3420

tattctgtcc tagtagtggc aaaagttgag aagggaaaat ccaagaaact gaagtcagtc     3480

aaagaattat tggggataac gattatggag cgctcgtctt ttgaaaagaa ccccatcgac     3540

ttccttgagg cgaaaggtta caaggaagta aaaaaggatc tcataattaa actaccaaag     3600

tatagtctgt ttgagttaga aaatggccga aaacggatgt tggctagcgc cggagagctt     3660

caaaagggga acgaactcgc actaccgtct aaatacgtga atttcctgta tttagcgtcc     3720

cattacgaga agttgaaagg ttcacctgaa gataacgaac agaagcaact ttttgttgag     3780

cagcacaaac attatctcga cgaaatcata gagcaaattt cggaattcag taagagagtc     3840

atcctagctg atgccaatct ggacaaagta ttaagcgcat acaacaagca cagggataaa     3900

cccatacgtg agcaggcgga aaatattatc catttgttta ctcttaccaa cctcggcgct     3960

ccagccgcat tcaagtattt tgacacaacg atagatcgca aacgatacac ttctaccaag     4020

gaggtgctag acgcgacact gattcaccaa tccatcacgg gattatatga aactcggata     4080

gatttgtcac agcttggggg tgac                                            4104


<210>  28
<211>  128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA scaffold expression cassette (S. pyogenes), containing a 
       20-nucleotide programmable sequence, a CRISPR RNA motif, and an 
       MS2 operator motif


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  20-nucleotide programmable sequence

<220>
<221>  misc_feature
<222>  (21)..(96)
<223>  CRISPR RNA motif (tracrRNA)

<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  GC linker

<220>
<221>  misc_feature
<222>  (99)..(100)
<223>  Extension

<220>
<221>  misc_feature
<222>  (101)..(119)
<223>  MS2 motif

<220>
<221>  misc_feature
<222>  (120)..(121)
<223>  Extension

<220>
<221>  misc_feature
<222>  (122)..(128)
<223>  Terminator

<400>  28
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgcgc acatgaggat cacccatgtg      120

cttttttt                                                               128


<210>  29
<211>  150
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA scaffold containing two MS2 loops (2xMS2)


<220>
<221>  misc_feature
<222>  (83)..(101)
<223>  MS2 scaffold

<220>
<221>  misc_feature
<222>  (112)..(130)
<223>  MS2 scaffold

<400>  29
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgcggga gcacatgagg atcacccatg tgccacgagc gacatgagga      120

tcacccatgt cgctcgtgtt cccttttttt                                       150


<210>  30
<211>  340
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Effector AID -MCP fusion


<220>
<221>  MISC_FEATURE
<222>  (1)..(198)
<223>  AID

<220>
<221>  MISC_FEATURE
<222>  (199)..(223)
<223>  MCP

<220>
<221>  MISC_FEATURE
<222>  (224)..(340)
<223>  UGI

<400>  30

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr 
        195                 200                 205             


His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Met 
    210                 215                 220                 


Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp Asn Gly Gly Thr Gly 
225                 230                 235                 240 


Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn Gly Ile Ala Glu Trp 
                245                 250                 255     


Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys Val Thr Cys Ser Val 
            260                 265                 270         


Arg Gln Ser Ser Ala Gln Asn Arg Lys Tyr Thr Ile Lys Val Glu Val 
        275                 280                 285             


Pro Lys Gly Ala Trp Arg Ser Tyr Leu Asn Met Glu Leu Thr Ile Pro 
    290                 295                 300                 


Ile Phe Ala Thr Asn Ser Asp Cys Glu Leu Ile Val Lys Ala Met Gln 
305                 310                 315                 320 


Gly Leu Leu Lys Asp Gly Asn Pro Ile Pro Ser Ala Ile Ala Ala Asn 
                325                 330                 335     


Ser Gly Ile Tyr 
            340 


<210>  31
<211>  594
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  wtAID cDNA


<220>
<221>  misc_feature
<222>  (112)..(114)
<223>  Ser38 codon

<400>  31
atggacagcc tcttgatgaa ccggaggaag tttctttacc aattcaaaaa tgtccgctgg       60

gctaagggtc ggcgtgagac ctacctgtgc tacgtagtga agaggcgtga cagtgctaca      120

tccttttcac tggactttgg ttatcttcgc aataagaacg gctgccacgt ggaattgctc      180

ttcctccgct acatctcgga ctgggaccta gaccctggcc gctgctaccg cgtcacctgg      240

ttcacctcct ggagcccctg ctacgactgt gcccgacatg tggccgactt tctgcgaggg      300

aaccccaacc tcagtctgag gatcttcacc gcgcgcctct acttctgtga ggaccgcaag      360

gctgagcccg aggggctgcg gcggctgcac cgcgccgggg tgcaaatagc catcatgacc      420

ttcaaagatt atttttactg ctggaatact tttgtagaaa accatgaaag aactttcaaa      480

gcctgggaag ggctgcatga aaattcagtt cgtctctcca gacagcttcg gcgcatcctt      540

ttgcccctgt atgaggttga tgacttacga gacgcatttc gtactttggg actt            594


<210>  32
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wtAID protein


<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  Ser38

<400>  32

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ser Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210>  33
<211>  594
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AID_S38A cDNA


<220>
<221>  misc_feature
<222>  (112)..(114)
<223>  S38A mutation

<400>  33
atggacagcc tcttgatgaa ccggaggaag tttctttacc aattcaaaaa tgtccgctgg       60

gctaagggtc ggcgtgagac ctacctgtgc tacgtagtga agaggcgtga cgccgctaca      120

tccttttcac tggactttgg ttatcttcgc aataagaacg gctgccacgt ggaattgctc      180

ttcctccgct acatctcgga ctgggaccta gaccctggcc gctgctaccg cgtcacctgg      240

ttcacctcct ggagcccctg ctacgactgt gcccgacatg tggccgactt tctgcgaggg      300

aaccccaacc tcagtctgag gatcttcacc gcgcgcctct acttctgtga ggaccgcaag      360

gctgagcccg aggggctgcg gcggctgcac cgcgccgggg tgcaaatagc catcatgacc      420

ttcaaagatt atttttactg ctggaatact tttgtagaaa accatgaaag aactttcaaa      480

gcctgggaag ggctgcatga aaattcagtt cgtctctcca gacagcttcg gcgcatcctt      540

ttgcccctgt atgaggttga tgacttacga gacgcatttc gtactttggg actt            594


<210>  34
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AID_S38A protein


<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  S38A mutation

<400>  34

Met Asp Ser Leu Leu Met Asn Arg Arg Lys Phe Leu Tyr Gln Phe Lys 
1               5                   10                  15      


Asn Val Arg Trp Ala Lys Gly Arg Arg Glu Thr Tyr Leu Cys Tyr Val 
            20                  25                  30          


Val Lys Arg Arg Asp Ala Ala Thr Ser Phe Ser Leu Asp Phe Gly Tyr 
        35                  40                  45              


Leu Arg Asn Lys Asn Gly Cys His Val Glu Leu Leu Phe Leu Arg Tyr 
    50                  55                  60                  


Ile Ser Asp Trp Asp Leu Asp Pro Gly Arg Cys Tyr Arg Val Thr Trp 
65                  70                  75                  80  


Phe Thr Ser Trp Ser Pro Cys Tyr Asp Cys Ala Arg His Val Ala Asp 
                85                  90                  95      


Phe Leu Arg Gly Asn Pro Asn Leu Ser Leu Arg Ile Phe Thr Ala Arg 
            100                 105                 110         


Leu Tyr Phe Cys Glu Asp Arg Lys Ala Glu Pro Glu Gly Leu Arg Arg 
        115                 120                 125             


Leu His Arg Ala Gly Val Gln Ile Ala Ile Met Thr Phe Lys Asp Tyr 
    130                 135                 140                 


Phe Tyr Cys Trp Asn Thr Phe Val Glu Asn His Glu Arg Thr Phe Lys 
145                 150                 155                 160 


Ala Trp Glu Gly Leu His Glu Asn Ser Val Arg Leu Ser Arg Gln Leu 
                165                 170                 175     


Arg Arg Ile Leu Leu Pro Leu Tyr Glu Val Asp Asp Leu Arg Asp Ala 
            180                 185                 190         


Phe Arg Thr Leu Gly Leu 
        195             


<210>  35
<211>  1835
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of ARNA scaffold mediated recruitment system nu 
       construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Nuclear Localization Signal (NLS)

<220>
<221>  MISC_FEATURE
<222>  (8)..(205)
<223>  AID

<220>
<221>  MISC_FEATURE
<222>  (206)..(230)
<223>  Linker

<220>
<221>  MISC_FEATURE
<222>  (231)..(347)
<223>  MCP

<220>
<221>  MISC_FEATURE
<222>  (351)..(368)
<223>  T2A peptide

<220>
<221>  MISC_FEATURE
<222>  (371)..(1737)
<223>  nCAS9D10A

<220>
<221>  MISC_FEATURE
<222>  (1742)..(1824)
<223>  UGI

<220>
<221>  MISC_FEATURE
<222>  (1829)..(1835)
<223>  Nuclear Localization Signal (NLS)

<400>  35

Pro Lys Lys Lys Arg Lys Val Met Asp Ser Leu Leu Met Asn Arg Arg 
1               5                   10                  15      


Lys Phe Leu Tyr Gln Phe Lys Asn Val Arg Trp Ala Lys Gly Arg Arg 
            20                  25                  30          


Glu Thr Tyr Leu Cys Tyr Val Val Lys Arg Arg Asp Ser Ala Thr Ser 
        35                  40                  45              


Phe Ser Leu Asp Phe Gly Tyr Leu Arg Asn Lys Asn Gly Cys His Val 
    50                  55                  60                  


Glu Leu Leu Phe Leu Arg Tyr Ile Ser Asp Trp Asp Leu Asp Pro Gly 
65                  70                  75                  80  


Arg Cys Tyr Arg Val Thr Trp Phe Thr Ser Trp Ser Pro Cys Tyr Asp 
                85                  90                  95      


Cys Ala Arg His Val Ala Asp Phe Leu Arg Gly Asn Pro Asn Leu Ser 
            100                 105                 110         


Leu Arg Ile Phe Thr Ala Arg Leu Tyr Phe Cys Glu Asp Arg Lys Ala 
        115                 120                 125             


Glu Pro Glu Gly Leu Arg Arg Leu His Arg Ala Gly Val Gln Ile Ala 
    130                 135                 140                 


Ile Met Thr Phe Lys Asp Tyr Phe Tyr Cys Trp Asn Thr Phe Val Glu 
145                 150                 155                 160 


Asn His Glu Arg Thr Phe Lys Ala Trp Glu Gly Leu His Glu Asn Ser 
                165                 170                 175     


Val Arg Leu Ser Arg Gln Leu Arg Arg Ile Leu Leu Pro Leu Tyr Glu 
            180                 185                 190         


Val Asp Asp Leu Arg Asp Ala Phe Arg Thr Leu Gly Leu Glu Leu Lys 
        195                 200                 205             


Thr Pro Leu Gly Asp Thr Thr His Thr Ser Pro Pro Cys Pro Ala Pro 
    210                 215                 220                 


Glu Leu Leu Gly Gly Pro Met Ala Ser Asn Phe Thr Gln Phe Val Leu 
225                 230                 235                 240 


Val Asp Asn Gly Gly Thr Gly Asp Val Thr Val Ala Pro Ser Asn Phe 
                245                 250                 255     


Ala Asn Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala 
            260                 265                 270         


Tyr Lys Val Thr Cys Ser Val Arg Gln Ser Ser Ala Gln Asn Arg Lys 
        275                 280                 285             


Tyr Thr Ile Lys Val Glu Val Pro Lys Gly Ala Trp Arg Ser Tyr Leu 
    290                 295                 300                 


Asn Met Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn Ser Asp Cys Glu 
305                 310                 315                 320 


Leu Ile Val Lys Ala Met Gln Gly Leu Leu Lys Asp Gly Asn Pro Ile 
                325                 330                 335     


Pro Ser Ala Ile Ala Ala Asn Ser Gly Ile Tyr Gly Ser Gly Glu Gly 
            340                 345                 350         


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
        355                 360                 365             


Gly Thr Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser 
    370                 375                 380                 


Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys 
385                 390                 395                 400 


Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu 
                405                 410                 415     


Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg 
            420                 425                 430         


Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile 
        435                 440                 445             


Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp 
    450                 455                 460                 


Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys 
465                 470                 475                 480 


Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala 
                485                 490                 495     


Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val 
            500                 505                 510         


Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala 
        515                 520                 525             


His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn 
    530                 535                 540                 


Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr 
545                 550                 555                 560 


Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp 
                565                 570                 575     


Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu 
            580                 585                 590         


Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly 
        595                 600                 605             


Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn 
    610                 615                 620                 


Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr 
625                 630                 635                 640 


Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala 
                645                 650                 655     


Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser 
            660                 665                 670         


Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala 
        675                 680                 685             


Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu 
    690                 695                 700                 


Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe 
705                 710                 715                 720 


Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala 
                725                 730                 735     


Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met 
            740                 745                 750         


Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu 
        755                 760                 765             


Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His 
    770                 775                 780                 


Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro 
785                 790                 795                 800 


Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg 
                805                 810                 815     


Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala 
            820                 825                 830         


Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu 
        835                 840                 845             


Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met 
    850                 855                 860                 


Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His 
865                 870                 875                 880 


Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val 
                885                 890                 895     


Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu 
            900                 905                 910         


Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val 
        915                 920                 925             


Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe 
    930                 935                 940                 


Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu 
945                 950                 955                 960 


Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu 
                965                 970                 975     


Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu 
            980                 985                 990         


Thr Leu Phe Glu Asp Arg Glu Met  Ile Glu Glu Arg Leu  Lys Thr Tyr 
        995                 1000                 1005             


Ala His  Leu Phe Asp Asp Lys  Val Met Lys Gln Leu  Lys Arg Arg 
    1010                 1015                 1020             


Arg Tyr  Thr Gly Trp Gly Arg  Leu Ser Arg Lys Leu  Ile Asn Gly 
    1025                 1030                 1035             


Ile Arg  Asp Lys Gln Ser Gly  Lys Thr Ile Leu Asp  Phe Leu Lys 
    1040                 1045                 1050             


Ser Asp  Gly Phe Ala Asn Arg  Asn Phe Met Gln Leu  Ile His Asp 
    1055                 1060                 1065             


Asp Ser  Leu Thr Phe Lys Glu  Asp Ile Gln Lys Ala  Gln Val Ser 
    1070                 1075                 1080             


Gly Gln  Gly Asp Ser Leu His  Glu His Ile Ala Asn  Leu Ala Gly 
    1085                 1090                 1095             


Ser Pro  Ala Ile Lys Lys Gly  Ile Leu Gln Thr Val  Lys Val Val 
    1100                 1105                 1110             


Asp Glu  Leu Val Lys Val Met  Gly Arg His Lys Pro  Glu Asn Ile 
    1115                 1120                 1125             


Val Ile  Glu Met Ala Arg Glu  Asn Gln Thr Thr Gln  Lys Gly Gln 
    1130                 1135                 1140             


Lys Asn  Ser Arg Glu Arg Met  Lys Arg Ile Glu Glu  Gly Ile Lys 
    1145                 1150                 1155             


Glu Leu  Gly Ser Gln Ile Leu  Lys Glu His Pro Val  Glu Asn Thr 
    1160                 1165                 1170             


Gln Leu  Gln Asn Glu Lys Leu  Tyr Leu Tyr Tyr Leu  Gln Asn Gly 
    1175                 1180                 1185             


Arg Asp  Met Tyr Val Asp Gln  Glu Leu Asp Ile Asn  Arg Leu Ser 
    1190                 1195                 1200             


Asp Tyr  Asp Val Asp His Ile  Val Pro Gln Ser Phe  Leu Lys Asp 
    1205                 1210                 1215             


Asp Ser  Ile Asp Asn Lys Val  Leu Thr Arg Ser Asp  Lys Asn Arg 
    1220                 1225                 1230             


Gly Lys  Ser Asp Asn Val Pro  Ser Glu Glu Val Val  Lys Lys Met 
    1235                 1240                 1245             


Lys Asn  Tyr Trp Arg Gln Leu  Leu Asn Ala Lys Leu  Ile Thr Gln 
    1250                 1255                 1260             


Arg Lys  Phe Asp Asn Leu Thr  Lys Ala Glu Arg Gly  Gly Leu Ser 
    1265                 1270                 1275             


Glu Leu  Asp Lys Ala Gly Phe  Ile Lys Arg Gln Leu  Val Glu Thr 
    1280                 1285                 1290             


Arg Gln  Ile Thr Lys His Val  Ala Gln Ile Leu Asp  Ser Arg Met 
    1295                 1300                 1305             


Asn Thr  Lys Tyr Asp Glu Asn  Asp Lys Leu Ile Arg  Glu Val Lys 
    1310                 1315                 1320             


Val Ile  Thr Leu Lys Ser Lys  Leu Val Ser Asp Phe  Arg Lys Asp 
    1325                 1330                 1335             


Phe Gln  Phe Tyr Lys Val Arg  Glu Ile Asn Asn Tyr  His His Ala 
    1340                 1345                 1350             


His Asp  Ala Tyr Leu Asn Ala  Val Val Gly Thr Ala  Leu Ile Lys 
    1355                 1360                 1365             


Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val Tyr Gly  Asp Tyr Lys 
    1370                 1375                 1380             


Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys Ser Glu  Gln Glu Ile 
    1385                 1390                 1395             


Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr Ser Asn  Ile Met Asn 
    1400                 1405                 1410             


Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn Gly Glu  Ile Arg Lys 
    1415                 1420                 1425             


Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr Gly Glu  Ile Val Trp 
    1430                 1435                 1440             


Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg Lys Val  Leu Ser Met 
    1445                 1450                 1455             


Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu Val Gln  Thr Gly Gly 
    1460                 1465                 1470             


Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg Asn Ser  Asp Lys Leu 
    1475                 1480                 1485             


Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys Lys Tyr  Gly Gly Phe 
    1490                 1495                 1500             


Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu Val Val  Ala Lys Val 
    1505                 1510                 1515             


Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser Val Lys  Glu Leu Leu 
    1520                 1525                 1530             


Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe Glu Lys  Asn Pro Ile 
    1535                 1540                 1545             


Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu Val Lys  Lys Asp Leu 
    1550                 1555                 1560             


Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe Glu Leu  Glu Asn Gly 
    1565                 1570                 1575             


Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu Leu Gln  Lys Gly Asn 
    1580                 1585                 1590             


Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn Phe Leu  Tyr Leu Ala 
    1595                 1600                 1605             


Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro Glu Asp  Asn Glu Gln 
    1610                 1615                 1620             


Lys Gln  Leu Phe Val Glu Gln  His Lys His Tyr Leu  Asp Glu Ile 
    1625                 1630                 1635             


Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg Val Ile  Leu Ala Asp 
    1640                 1645                 1650             


Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr Asn Lys  His Arg Asp 
    1655                 1660                 1665             


Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile Ile His  Leu Phe Thr 
    1670                 1675                 1680             


Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe Lys Tyr  Phe Asp Thr 
    1685                 1690                 1695             


Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr Lys Glu  Val Leu Asp 
    1700                 1705                 1710             


Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly Leu Tyr  Glu Thr Arg 
    1715                 1720                 1725             


Ile Asp  Leu Ser Gln Leu Gly  Gly Asp Ser Gly Gly  Ser Thr Asn 
    1730                 1735                 1740             


Leu Ser  Asp Ile Ile Glu Lys  Glu Thr Gly Lys Gln  Leu Val Ile 
    1745                 1750                 1755             


Gln Glu  Ser Ile Leu Met Leu  Pro Glu Glu Val Glu  Glu Val Ile 
    1760                 1765                 1770             


Gly Asn  Lys Pro Glu Ser Asp  Ile Leu Val His Thr  Ala Tyr Asp 
    1775                 1780                 1785             


Glu Ser  Thr Asp Glu Asn Val  Met Leu Leu Thr Ser  Asp Ala Pro 
    1790                 1795                 1800             


Glu Tyr  Lys Pro Trp Ala Leu  Val Ile Gln Asp Ser  Asn Gly Glu 
    1805                 1810                 1815             


Asn Lys  Ile Lys Met Leu Ser  Gly Gly Ser Pro Lys  Lys Lys Arg 
    1820                 1825                 1830             


Lys Val  
    1835 


<210>  36
<211>  1941
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of ARNA scaffold mediated recruitment system 
       nu.2 construct


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Nuclear Localization Signal (NLS)

<220>
<221>  MISC_FEATURE
<222>  (8)..(205)
<223>  AID

<220>
<221>  MISC_FEATURE
<222>  (206)..(230)
<223>  Linker

<220>
<221>  MISC_FEATURE
<222>  (231)..(347)
<223>  MCP

<220>
<221>  MISC_FEATURE
<222>  (351)..(368)
<223>  T2A peptide

<220>
<221>  MISC_FEATURE
<222>  (371)..(377)
<223>  Nuclear Localization Signal (NLS)

<220>
<221>  MISC_FEATURE
<222>  (378)..(1744)
<223>  nCAS9D10A

<220>
<221>  MISC_FEATURE
<222>  (1755)..(1837)
<223>  UGI

<220>
<221>  MISC_FEATURE
<222>  (1848)..(1930)
<223>  UGI

<220>
<221>  MISC_FEATURE
<222>  (1935)..(1941)
<223>  Nuclear Localization Signal (NLS)

<400>  36

Pro Lys Lys Lys Arg Lys Val Met Asp Ser Leu Leu Met Asn Arg Arg 
1               5                   10                  15      


Lys Phe Leu Tyr Gln Phe Lys Asn Val Arg Trp Ala Lys Gly Arg Arg 
            20                  25                  30          


Glu Thr Tyr Leu Cys Tyr Val Val Lys Arg Arg Asp Ser Ala Thr Ser 
        35                  40                  45              


Phe Ser Leu Asp Phe Gly Tyr Leu Arg Asn Lys Asn Gly Cys His Val 
    50                  55                  60                  


Glu Leu Leu Phe Leu Arg Tyr Ile Ser Asp Trp Asp Leu Asp Pro Gly 
65                  70                  75                  80  


Arg Cys Tyr Arg Val Thr Trp Phe Thr Ser Trp Ser Pro Cys Tyr Asp 
                85                  90                  95      


Cys Ala Arg His Val Ala Asp Phe Leu Arg Gly Asn Pro Asn Leu Ser 
            100                 105                 110         


Leu Arg Ile Phe Thr Ala Arg Leu Tyr Phe Cys Glu Asp Arg Lys Ala 
        115                 120                 125             


Glu Pro Glu Gly Leu Arg Arg Leu His Arg Ala Gly Val Gln Ile Ala 
    130                 135                 140                 


Ile Met Thr Phe Lys Asp Tyr Phe Tyr Cys Trp Asn Thr Phe Val Glu 
145                 150                 155                 160 


Asn His Glu Arg Thr Phe Lys Ala Trp Glu Gly Leu His Glu Asn Ser 
                165                 170                 175     


Val Arg Leu Ser Arg Gln Leu Arg Arg Ile Leu Leu Pro Leu Tyr Glu 
            180                 185                 190         


Val Asp Asp Leu Arg Asp Ala Phe Arg Thr Leu Gly Leu Glu Leu Lys 
        195                 200                 205             


Thr Pro Leu Gly Asp Thr Thr His Thr Ser Pro Pro Cys Pro Ala Pro 
    210                 215                 220                 


Glu Leu Leu Gly Gly Pro Met Ala Ser Asn Phe Thr Gln Phe Val Leu 
225                 230                 235                 240 


Val Asp Asn Gly Gly Thr Gly Asp Val Thr Val Ala Pro Ser Asn Phe 
                245                 250                 255     


Ala Asn Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala 
            260                 265                 270         


Tyr Lys Val Thr Cys Ser Val Arg Gln Ser Ser Ala Gln Asn Arg Lys 
        275                 280                 285             


Tyr Thr Ile Lys Val Glu Val Pro Lys Gly Ala Trp Arg Ser Tyr Leu 
    290                 295                 300                 


Asn Met Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn Ser Asp Cys Glu 
305                 310                 315                 320 


Leu Ile Val Lys Ala Met Gln Gly Leu Leu Lys Asp Gly Asn Pro Ile 
                325                 330                 335     


Pro Ser Ala Ile Ala Ala Asn Ser Gly Ile Tyr Gly Ser Gly Glu Gly 
            340                 345                 350         


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
        355                 360                 365             


Gly Thr Pro Lys Lys Lys Arg Lys Val Asp Lys Lys Tyr Ser Ile Gly 
    370                 375                 380                 


Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu 
385                 390                 395                 400 


Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg 
                405                 410                 415     


His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly 
            420                 425                 430         


Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr 
        435                 440                 445             


Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn 
    450                 455                 460                 


Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser 
465                 470                 475                 480 


Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly 
                485                 490                 495     


Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr 
            500                 505                 510         


His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg 
        515                 520                 525             


Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe 
    530                 535                 540                 


Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu 
545                 550                 555                 560 


Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro 
                565                 570                 575     


Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu 
            580                 585                 590         


Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu 
        595                 600                 605             


Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu 
    610                 615                 620                 


Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu 
625                 630                 635                 640 


Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala 
                645                 650                 655     


Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu 
            660                 665                 670         


Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile 
        675                 680                 685             


Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His 
    690                 695                 700                 


His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro 
705                 710                 715                 720 


Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala 
                725                 730                 735     


Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile 
            740                 745                 750         


Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys 
        755                 760                 765             


Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly 
    770                 775                 780                 


Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg 
785                 790                 795                 800 


Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile 
                805                 810                 815     


Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala 
            820                 825                 830         


Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr 
        835                 840                 845             


Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala 
    850                 855                 860                 


Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn 
865                 870                 875                 880 


Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val 
                885                 890                 895     


Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys 
            900                 905                 910         


Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu 
        915                 920                 925             


Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr 
    930                 935                 940                 


Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu 
945                 950                 955                 960 


Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile 
                965                 970                 975     


Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu 
            980                 985                 990         


Glu Asp Ile Val Leu Thr Leu Thr  Leu Phe Glu Asp Arg  Glu Met Ile 
        995                 1000                 1005             


Glu Glu  Arg Leu Lys Thr Tyr  Ala His Leu Phe Asp  Asp Lys Val 
    1010                 1015                 1020             


Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr Gly Trp  Gly Arg Leu 
    1025                 1030                 1035             


Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp Lys Gln  Ser Gly Lys 
    1040                 1045                 1050             


Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly Phe Ala  Asn Arg Asn 
    1055                 1060                 1065             


Phe Met  Gln Leu Ile His Asp  Asp Ser Leu Thr Phe  Lys Glu Asp 
    1070                 1075                 1080             


Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly Asp Ser  Leu His Glu 
    1085                 1090                 1095             


His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala Ile Lys  Lys Gly Ile 
    1100                 1105                 1110             


Leu Gln  Thr Val Lys Val Val  Asp Glu Leu Val Lys  Val Met Gly 
    1115                 1120                 1125             


Arg His  Lys Pro Glu Asn Ile  Val Ile Glu Met Ala  Arg Glu Asn 
    1130                 1135                 1140             


Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser Arg Glu  Arg Met Lys 
    1145                 1150                 1155             


Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly Ser Gln  Ile Leu Lys 
    1160                 1165                 1170             


Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn Glu  Lys Leu Tyr 
    1175                 1180                 1185             


Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  Asp Gln Glu 
    1190                 1195                 1200             


Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His Ile Val 
    1205                 1210                 1215             


Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val Leu 
    1220                 1225                 1230             


Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
    1235                 1240                 1245             


Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu 
    1250                 1255                 1260             


Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys 
    1265                 1270                 1275             


Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile 
    1280                 1285                 1290             


Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile Thr Lys  His Val Ala 
    1295                 1300                 1305             


Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp 
    1310                 1315                 1320             


Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu 
    1325                 1330                 1335             


Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe Tyr Lys  Val Arg Glu 
    1340                 1345                 1350             


Ile Asn  Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
    1355                 1360                 1365             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1370                 1375                 1380             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1385                 1390                 1395             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1400                 1405                 1410             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1415                 1420                 1425             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1430                 1435                 1440             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1445                 1450                 1455             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1460                 1465                 1470             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1475                 1480                 1485             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1490                 1495                 1500             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1505                 1510                 1515             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1520                 1525                 1530             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1535                 1540                 1545             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1550                 1555                 1560             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1565                 1570                 1575             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1580                 1585                 1590             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1595                 1600                 1605             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1610                 1615                 1620             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1625                 1630                 1635             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1640                 1645                 1650             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1655                 1660                 1665             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1670                 1675                 1680             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1685                 1690                 1695             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1700                 1705                 1710             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1715                 1720                 1725             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1730                 1735                 1740             


Asp Ser  Gly Gly Ser Gly Gly  Ser Gly Gly Ser Thr  Asn Leu Ser 
    1745                 1750                 1755             


Asp Ile  Ile Glu Lys Glu Thr  Gly Lys Gln Leu Val  Ile Gln Glu 
    1760                 1765                 1770             


Ser Ile  Leu Met Leu Pro Glu  Glu Val Glu Glu Val  Ile Gly Asn 
    1775                 1780                 1785             


Lys Pro  Glu Ser Asp Ile Leu  Val His Thr Ala Tyr  Asp Glu Ser 
    1790                 1795                 1800             


Thr Asp  Glu Asn Val Met Leu  Leu Thr Ser Asp Ala  Pro Glu Tyr 
    1805                 1810                 1815             


Lys Pro  Trp Ala Leu Val Ile  Gln Asp Ser Asn Gly  Glu Asn Lys 
    1820                 1825                 1830             


Ile Lys  Met Leu Ser Gly Gly  Ser Gly Gly Ser Gly  Gly Ser Thr 
    1835                 1840                 1845             


Asn Leu  Ser Asp Ile Ile Glu  Lys Glu Thr Gly Lys  Gln Leu Val 
    1850                 1855                 1860             


Ile Gln  Glu Ser Ile Leu Met  Leu Pro Glu Glu Val  Glu Glu Val 
    1865                 1870                 1875             


Ile Gly  Asn Lys Pro Glu Ser  Asp Ile Leu Val His  Thr Ala Tyr 
    1880                 1885                 1890             


Asp Glu  Ser Thr Asp Glu Asn  Val Met Leu Leu Thr  Ser Asp Ala 
    1895                 1900                 1905             


Pro Glu  Tyr Lys Pro Trp Ala  Leu Val Ile Gln Asp  Ser Asn Gly 
    1910                 1915                 1920             


Glu Asn  Lys Ile Lys Met Leu  Ser Gly Gly Ser Pro  Lys Lys Lys 
    1925                 1930                 1935             


Arg Lys  Val 
    1940     


<210>  37
<211>  1866
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of RNA scaffold mediated recruitment system


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Nuclear Localization Signal (NLS)

<220>
<221>  MISC_FEATURE
<222>  (8)..(235)
<223>  AID

<220>
<221>  MISC_FEATURE
<222>  (236)..(261)
<223>  Linker

<220>
<221>  MISC_FEATURE
<222>  (262)..(378)
<223>  MCP

<220>
<221>  MISC_FEATURE
<222>  (382)..(399)
<223>  T2A peptide

<220>
<221>  MISC_FEATURE
<222>  (402)..(1768)
<223>  nCAS9D10A

<220>
<221>  MISC_FEATURE
<222>  (1772)..(1855)
<223>  UGI

<220>
<221>  MISC_FEATURE
<222>  (1860)..(1866)
<223>  Nuclear Localization Signal (NLS)

<400>  37

Pro Lys Lys Lys Arg Lys Val Met Ser Ser Glu Thr Gly Pro Val Ala 
1               5                   10                  15      


Val Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu Phe Glu Val 
            20                  25                  30          


Phe Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu Leu Tyr Glu 
        35                  40                  45              


Ile Asn Trp Gly Gly Arg His Ser Ile Trp Arg His Thr Ser Gln Asn 
    50                  55                  60                  


Thr Asn Lys His Val Glu Val Asn Phe Ile Glu Lys Phe Thr Thr Glu 
65                  70                  75                  80  


Arg Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr Trp Phe Leu Ser 
                85                  90                  95      


Trp Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu Phe Leu Ser 
            100                 105                 110         


Arg Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala Arg Leu Tyr His 
        115                 120                 125             


His Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp Leu Ile Ser Ser 
    130                 135                 140                 


Gly Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser Gly Tyr Cys Trp 
145                 150                 155                 160 


Arg Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala His Trp Pro Arg 
                165                 170                 175     


Tyr Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu Leu Tyr Cys Ile 
            180                 185                 190         


Ile Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys Gln Pro 
        195                 200                 205             


Gln Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His Tyr Gln Arg 
    210                 215                 220                 


Leu Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Glu Leu Lys Thr 
225                 230                 235                 240 


Pro Leu Gly Asp Thr Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val 
            260                 265                 270         


Asp Asn Gly Gly Thr Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala 
        275                 280                 285             


Asn Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr 
    290                 295                 300                 


Lys Val Thr Cys Ser Val Arg Gln Ser Ser Ala Gln Asn Arg Lys Tyr 
305                 310                 315                 320 


Thr Ile Lys Val Glu Val Pro Lys Gly Ala Trp Arg Ser Tyr Leu Asn 
                325                 330                 335     


Met Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn Ser Asp Cys Glu Leu 
            340                 345                 350         


Ile Val Lys Ala Met Gln Gly Leu Leu Lys Asp Gly Asn Pro Ile Pro 
        355                 360                 365             


Ser Ala Ile Ala Ala Asn Ser Gly Ile Tyr Gly Ser Gly Glu Gly Arg 
    370                 375                 380                 


Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Gly 
385                 390                 395                 400 


Thr Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val 
                405                 410                 415     


Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe 
            420                 425                 430         


Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile 
        435                 440                 445             


Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu 
    450                 455                 460                 


Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys 
465                 470                 475                 480 


Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser 
                485                 490                 495     


Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys 
            500                 505                 510         


His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr 
        515                 520                 525             


His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp 
    530                 535                 540                 


Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His 
545                 550                 555                 560 


Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro 
                565                 570                 575     


Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr 
            580                 585                 590         


Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala 
        595                 600                 605             


Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn 
    610                 615                 620                 


Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn 
625                 630                 635                 640 


Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe 
                645                 650                 655     


Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp 
            660                 665                 670         


Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp 
        675                 680                 685             


Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp 
    690                 695                 700                 


Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser 
705                 710                 715                 720 


Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys 
                725                 730                 735     


Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe 
            740                 745                 750         


Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser 
        755                 760                 765             


Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp 
    770                 775                 780                 


Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg 
785                 790                 795                 800 


Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu 
                805                 810                 815     


Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe 
            820                 825                 830         


Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile 
        835                 840                 845             


Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp 
    850                 855                 860                 


Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu 
865                 870                 875                 880 


Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr 
                885                 890                 895     


Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser 
            900                 905                 910         


Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys 
        915                 920                 925             


Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln 
    930                 935                 940                 


Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr 
945                 950                 955                 960 


Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp 
                965                 970                 975     


Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly 
            980                 985                 990         


Thr Tyr His Asp Leu Leu Lys Ile  Ile Lys Asp Lys Asp  Phe Leu Asp 
        995                 1000                 1005             


Asn Glu  Glu Asn Glu Asp Ile  Leu Glu Asp Ile Val  Leu Thr Leu 
    1010                 1015                 1020             


Thr Leu  Phe Glu Asp Arg Glu  Met Ile Glu Glu Arg  Leu Lys Thr 
    1025                 1030                 1035             


Tyr Ala  His Leu Phe Asp Asp  Lys Val Met Lys Gln  Leu Lys Arg 
    1040                 1045                 1050             


Arg Arg  Tyr Thr Gly Trp Gly  Arg Leu Ser Arg Lys  Leu Ile Asn 
    1055                 1060                 1065             


Gly Ile  Arg Asp Lys Gln Ser  Gly Lys Thr Ile Leu  Asp Phe Leu 
    1070                 1075                 1080             


Lys Ser  Asp Gly Phe Ala Asn  Arg Asn Phe Met Gln  Leu Ile His 
    1085                 1090                 1095             


Asp Asp  Ser Leu Thr Phe Lys  Glu Asp Ile Gln Lys  Ala Gln Val 
    1100                 1105                 1110             


Ser Gly  Gln Gly Asp Ser Leu  His Glu His Ile Ala  Asn Leu Ala 
    1115                 1120                 1125             


Gly Ser  Pro Ala Ile Lys Lys  Gly Ile Leu Gln Thr  Val Lys Val 
    1130                 1135                 1140             


Val Asp  Glu Leu Val Lys Val  Met Gly Arg His Lys  Pro Glu Asn 
    1145                 1150                 1155             


Ile Val  Ile Glu Met Ala Arg  Glu Asn Gln Thr Thr  Gln Lys Gly 
    1160                 1165                 1170             


Gln Lys  Asn Ser Arg Glu Arg  Met Lys Arg Ile Glu  Glu Gly Ile 
    1175                 1180                 1185             


Lys Glu  Leu Gly Ser Gln Ile  Leu Lys Glu His Pro  Val Glu Asn 
    1190                 1195                 1200             


Thr Gln  Leu Gln Asn Glu Lys  Leu Tyr Leu Tyr Tyr  Leu Gln Asn 
    1205                 1210                 1215             


Gly Arg  Asp Met Tyr Val Asp  Gln Glu Leu Asp Ile  Asn Arg Leu 
    1220                 1225                 1230             


Ser Asp  Tyr Asp Val Asp His  Ile Val Pro Gln Ser  Phe Leu Lys 
    1235                 1240                 1245             


Asp Asp  Ser Ile Asp Asn Lys  Val Leu Thr Arg Ser  Asp Lys Asn 
    1250                 1255                 1260             


Arg Gly  Lys Ser Asp Asn Val  Pro Ser Glu Glu Val  Val Lys Lys 
    1265                 1270                 1275             


Met Lys  Asn Tyr Trp Arg Gln  Leu Leu Asn Ala Lys  Leu Ile Thr 
    1280                 1285                 1290             


Gln Arg  Lys Phe Asp Asn Leu  Thr Lys Ala Glu Arg  Gly Gly Leu 
    1295                 1300                 1305             


Ser Glu  Leu Asp Lys Ala Gly  Phe Ile Lys Arg Gln  Leu Val Glu 
    1310                 1315                 1320             


Thr Arg  Gln Ile Thr Lys His  Val Ala Gln Ile Leu  Asp Ser Arg 
    1325                 1330                 1335             


Met Asn  Thr Lys Tyr Asp Glu  Asn Asp Lys Leu Ile  Arg Glu Val 
    1340                 1345                 1350             


Lys Val  Ile Thr Leu Lys Ser  Lys Leu Val Ser Asp  Phe Arg Lys 
    1355                 1360                 1365             


Asp Phe  Gln Phe Tyr Lys Val  Arg Glu Ile Asn Asn  Tyr His His 
    1370                 1375                 1380             


Ala His  Asp Ala Tyr Leu Asn  Ala Val Val Gly Thr  Ala Leu Ile 
    1385                 1390                 1395             


Lys Lys  Tyr Pro Lys Leu Glu  Ser Glu Phe Val Tyr  Gly Asp Tyr 
    1400                 1405                 1410             


Lys Val  Tyr Asp Val Arg Lys  Met Ile Ala Lys Ser  Glu Gln Glu 
    1415                 1420                 1425             


Ile Gly  Lys Ala Thr Ala Lys  Tyr Phe Phe Tyr Ser  Asn Ile Met 
    1430                 1435                 1440             


Asn Phe  Phe Lys Thr Glu Ile  Thr Leu Ala Asn Gly  Glu Ile Arg 
    1445                 1450                 1455             


Lys Arg  Pro Leu Ile Glu Thr  Asn Gly Glu Thr Gly  Glu Ile Val 
    1460                 1465                 1470             


Trp Asp  Lys Gly Arg Asp Phe  Ala Thr Val Arg Lys  Val Leu Ser 
    1475                 1480                 1485             


Met Pro  Gln Val Asn Ile Val  Lys Lys Thr Glu Val  Gln Thr Gly 
    1490                 1495                 1500             


Gly Phe  Ser Lys Glu Ser Ile  Leu Pro Lys Arg Asn  Ser Asp Lys 
    1505                 1510                 1515             


Leu Ile  Ala Arg Lys Lys Asp  Trp Asp Pro Lys Lys  Tyr Gly Gly 
    1520                 1525                 1530             


Phe Asp  Ser Pro Thr Val Ala  Tyr Ser Val Leu Val  Val Ala Lys 
    1535                 1540                 1545             


Val Glu  Lys Gly Lys Ser Lys  Lys Leu Lys Ser Val  Lys Glu Leu 
    1550                 1555                 1560             


Leu Gly  Ile Thr Ile Met Glu  Arg Ser Ser Phe Glu  Lys Asn Pro 
    1565                 1570                 1575             


Ile Asp  Phe Leu Glu Ala Lys  Gly Tyr Lys Glu Val  Lys Lys Asp 
    1580                 1585                 1590             


Leu Ile  Ile Lys Leu Pro Lys  Tyr Ser Leu Phe Glu  Leu Glu Asn 
    1595                 1600                 1605             


Gly Arg  Lys Arg Met Leu Ala  Ser Ala Gly Glu Leu  Gln Lys Gly 
    1610                 1615                 1620             


Asn Glu  Leu Ala Leu Pro Ser  Lys Tyr Val Asn Phe  Leu Tyr Leu 
    1625                 1630                 1635             


Ala Ser  His Tyr Glu Lys Leu  Lys Gly Ser Pro Glu  Asp Asn Glu 
    1640                 1645                 1650             


Gln Lys  Gln Leu Phe Val Glu  Gln His Lys His Tyr  Leu Asp Glu 
    1655                 1660                 1665             


Ile Ile  Glu Gln Ile Ser Glu  Phe Ser Lys Arg Val  Ile Leu Ala 
    1670                 1675                 1680             


Asp Ala  Asn Leu Asp Lys Val  Leu Ser Ala Tyr Asn  Lys His Arg 
    1685                 1690                 1695             


Asp Lys  Pro Ile Arg Glu Gln  Ala Glu Asn Ile Ile  His Leu Phe 
    1700                 1705                 1710             


Thr Leu  Thr Asn Leu Gly Ala  Pro Ala Ala Phe Lys  Tyr Phe Asp 
    1715                 1720                 1725             


Thr Thr  Ile Asp Arg Lys Arg  Tyr Thr Ser Thr Lys  Glu Val Leu 
    1730                 1735                 1740             


Asp Ala  Thr Leu Ile His Gln  Ser Ile Thr Gly Leu  Tyr Glu Thr 
    1745                 1750                 1755             


Arg Ile  Asp Leu Ser Gln Leu  Gly Gly Asp Ser Gly  Gly Ser Thr 
    1760                 1765                 1770             


Asn Leu  Ser Asp Ile Ile Glu  Lys Glu Thr Gly Lys  Gln Leu Val 
    1775                 1780                 1785             


Ile Gln  Glu Ser Ile Leu Met  Leu Pro Glu Glu Val  Glu Glu Val 
    1790                 1795                 1800             


Ile Gly  Asn Lys Pro Glu Ser  Asp Ile Leu Val His  Thr Ala Tyr 
    1805                 1810                 1815             


Asp Glu  Ser Thr Asp Glu Asn  Val Met Leu Leu Thr  Ser Asp Ala 
    1820                 1825                 1830             


Pro Glu  Tyr Lys Pro Trp Ala  Leu Val Ile Gln Asp  Ser Asn Gly 
    1835                 1840                 1845             


Glu Asn  Lys Ile Lys Met Leu  Ser Gly Gly Ser Pro  Lys Lys Lys 
    1850                 1855                 1860             


Arg Lys  Val 
    1865     


<210>  38
<211>  128
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  RNA sequence of gRNA_MS2 construct


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the customisable target

<220>
<221>  misc_feature
<222>  (21)..(96)
<223>  gRNA scaffold

<220>
<221>  misc_feature
<222>  (101)..(119)
<223>  MS2 aptamer

<400>  38
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcgcgc acaugaggau cacccaugug      120

cuuuuuuu                                                               128


<210>  39
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of gRNA_2xMS2 construct


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents customisable target sequence

<220>
<221>  misc_feature
<222>  (21)..(96)
<223>  gRNA scaffold

<220>
<221>  misc_feature
<222>  (103)..(121)
<223>  MS2 aptamers

<220>
<221>  misc_feature
<222>  (132)..(150)
<223>  MS2 aptamers

<220>
<221>  misc_feature
<222>  (164)..(170)
<223>  Terminator

<400>  39
nnnnnnnnnn nnnnnnnngu uuuagagcua gaaauagcaa guuaaaauaa ggcuaguccg       60

uuaucaacuu gaaaaagugg caccgagucg gugcgggagc acaugaggau cacccaugug      120

ccacgagcga caugaggauc acccaugucg ctcgtgttcc cuuuuuuu                   168


<210>  40
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. pyrogenes sgRNA


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(96)
<223>  Constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (97)..(103)
<223>  Terminator

<400>  40
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttt                        103


<210>  41
<211>  170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2xMS2_3'


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(96)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (97)..(102)
<223>  Extension

<220>
<221>  misc_feature
<222>  (103)..(121)
<223>  MS2 (C5 variant)

<220>
<221>  misc_feature
<222>  (122)..(131)
<223>  Extension

<220>
<221>  misc_feature
<222>  (132)..(150)
<223>  MS2 (C5 variant)

<220>
<221>  misc_feature
<222>  (151)..(163)
<223>  Extension

<220>
<221>  misc_feature
<222>  (164)..(170)
<223>  Terminator

<400>  41
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgcggga gcacatgagg atcacccatg      120

tgccacgagc gacatgagga tcacccatgt cgctcgtgtt cccttttttt                 170


<210>  42
<211>  133
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2_TL


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(37)
<223>  Extension

<220>
<221>  misc_feature
<222>  (38)..(56)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (57)..(66)
<223>  Extension

<220>
<221>  misc_feature
<222>  (67)..(126)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (127)..(133)
<223>  Terminator

<400>  42
nnnnnnnnnn nnnnnnnnnn gttttagagc taggccaaca tgaggatcac ccatgtctgc       60

agggcctagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt      120

cggtgctttt ttt                                                         133


<210>  43
<211>  128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2_3'


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N respresents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(96)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (97)..(100)
<223>  Extension

<220>
<221>  misc_feature
<222>  (101)..(119)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (120)..(121)
<223>  Extension

<220>
<221>  misc_feature
<222>  (122)..(128)
<223>  Terminator

<400>  43
nnnnnnnnnn nnnnnnnnnn gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgcgc acatgaggat cacccatgtg      120

cttttttt                                                               128


<210>  44
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  7bp Extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(39)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (40)..(43)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (44)..(50)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (51)..(110)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (111)..(117)
<223>  Terminator

<400>  44
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttg aaaaacagca tagcaagtta       60

aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttt         117


<210>  45
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2xMS2_3' 7bp_extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(39)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (40)..(43)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (44)..(50)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (51)..(110)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (111)..(116)
<223>  Extension

<220>
<221>  misc_feature
<222>  (117)..(135)
<223>  MS2 (C5 variant)

<220>
<221>  misc_feature
<222>  (136)..(145)
<223>  Extension

<220>
<221>  misc_feature
<222>  (146)..(164)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (165)..(177)
<223>  Extension

<220>
<221>  misc_feature
<222>  (178)..(184)
<223>  Terminator

<400>  45
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttg aaaaacagca tagcaagtta       60

aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc gggagcacat      120

gaggatcacc catgtgccac gagcgacatg aggatcaccc atgtcgctcg tgttcccttt      180

tttt                                                                   184


<210>  46
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2-TL _7bp extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the  20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(39)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (40)..(44)
<223>  Extension

<220>
<221>  misc_feature
<222>  (45)..(63)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (64)..(73)
<223>  Extension

<220>
<221>  misc_feature
<222>  (74)..(80)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (81)..(140)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (141)..(147)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (141)..(147)
<223>  Terminator

<400>  46
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttg gccaacatga ggatcaccca       60

tgtctgcagg gccaacagca tagcaagtta aaataaggct agtccgttat caacttgaaa      120

aagtggcacc gagtcggtgc ttttttt                                          147


<210>  47
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2-3'_2bp extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(34)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (35)..(38)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (39)..(40)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (41)..(100)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (101)..(104)
<223>  Extension

<220>
<221>  misc_feature
<222>  (105)..(123)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (124)..(126)
<223>  Extension

<220>
<221>  misc_feature
<222>  (127)..(132)
<223>  Terminator

<400>  47
nnnnnnnnnn nnnnnnnnnn gttttagagc tatggaaaca tagcaagtta aaataaggct       60

agtccgttat caacttgaaa aagtggcacc gagtcggtgc gcgcacatga ggatcaccca      120

tgtgcttttt tt                                                          132


<210>  48
<211>  138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2-3'_5bp-extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N represents the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(37)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (38)..(41)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (42)..(46)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (47)..(106)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (107)..(110)
<223>  Extension

<220>
<221>  misc_feature
<222>  (111)..(129)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (130)..(131)
<223>  Extension

<220>
<221>  misc_feature
<222>  (132)..(138)
<223>  Terminator

<400>  48
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctggaa acagcatagc aagttaaaat       60

aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgcgc acatgaggat      120

cacccatgtg cttttttt                                                    138


<210>  49
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2-3'_7bp-extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N denotes the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(39)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (40)..(43)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (44)..(50)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (51)..(110)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (111)..(114)
<223>  Extensions

<220>
<221>  misc_feature
<222>  (111)..(133)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (134)..(135)
<223>  Extension

<220>
<221>  misc_feature
<222>  (136)..(142)
<223>  Extension

<220>
<221>  misc_feature
<222>  (136)..(142)
<223>  Terminator

<400>  49
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttg aaaaacagca tagcaagtta       60

aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc gcgcacatga      120

ggatcaccca tgtgcttttt tt                                               142


<210>  50
<211>  148
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2-3'_10bp-extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N denotes the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(42)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (43)..(46)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (47)..(56)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (57)..(116)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (117)..(120)
<223>  Extension

<220>
<221>  misc_feature
<222>  (121)..(139)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (140)..(141)
<223>  Extension

<220>
<221>  misc_feature
<222>  (142)..(148)
<223>  Terminator

<400>  50
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttt tggaaacaaa acagcatagc       60

aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgcgc      120

acatgaggat cacccatgtg cttttttt                                         148


<210>  51
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1xMS2-SL2_7bp-extended_US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N denotes the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(39)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (40)..(43)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (44)..(50)
<223>  the extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (51)..(86)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (87)..(91)
<223>  Extension

<220>
<221>  misc_feature
<222>  (92)..(110)
<223>  MS2 (C5 variant) aptamer

<220>
<221>  misc_feature
<222>  (111)..(120)
<223>  Extension

<220>
<221>  misc_feature
<222>  (121)..(140)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (141)..(147)
<223>  Terminator

<400>  51
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttg aaaaacagca tagcaagtta       60

aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt ctgcagggcc      120

aagtggcacc gagtcggtgc ttttttt                                          147


<210>  52
<211>  167
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2xMS2_C5-SL2_f6-3'_7bp extended-US


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N denotes the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (21)..(32)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (33)..(39)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (40)..(43)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (44)..(50)
<223>  extended repeat: anti-repeat sequences

<220>
<221>  misc_feature
<222>  (51)..(86)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (87)..(91)
<223>  Extension

<220>
<221>  misc_feature
<222>  (92)..(110)
<223>  f6 aptamer

<220>
<221>  misc_feature
<222>  (111)..(120)
<223>  Extension

<220>
<221>  misc_feature
<222>  (121)..(140)
<223>  constant sgRNA sequence

<220>
<221>  misc_feature
<222>  (141)..(144)
<223>  Extension

<220>
<221>  misc_feature
<222>  (145)..(158)
<223>  f6 aptamer

<220>
<221>  misc_feature
<222>  (159)..(160)
<223>  Extension

<220>
<221>  misc_feature
<222>  (161)..(167)
<223>  Terminator

<400>  52
nnnnnnnnnn nnnnnnnnnn gttttagagc tatgctgttg aaaaacagca tagcaagtta       60

aaataaggct agtccgttat caacttggcc aacatgagga tcacccatgt ctgcagggcc      120

aagtggcacc gagtcggtgc gcgcccacag tcactggggc ttttttt                    167


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence Site2

<400>  53
gaacacaaag catagactgc                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence Site 3

<400>  54
ggcccagact gagcacgtga                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence CTNNB1

<400>  55
ctggactctg gaatccattc                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence EGFR

<400>  56
atcacgcagc tcatgccctt                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence PCSK9

<400>  57
caggttccac gggatgctct                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence FANCF

<400>  58
ggaatccctt ctgcagcacc                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence TRAC

<400>  59
ttcgtatctg taaaaccaag                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNa target site sequence B2M

<400>  60
cttaccccac ttaactatct                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences CR0118_PDCD1

<400>  61
cagttccaaa ccctggtggt                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences  CR0107_PDCD1

<400>  62
gggggttcca gggcctgtct                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences CR0057-TRAC_EX3

<400>  63
ttcgtatctg taaaaccaag                                                   20


<210>  64
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences CR0151_CD2

<400>  64
gttcagccaa aacctcccca                                                   20


<210>  65
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences CR0121_PDCD1

<400>  65
ggagtctgag agatggagag                                                   20


<210>  66
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences CR0165_CIITA

<400>  66
cagctcacag tgtgccacca                                                   20


<210>  67
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences TRAC_22550571

<400>  67
ttcaaaacct gtcagtgatt                                                   20


<210>  68
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences PDCD1_241852953

<400>  68
gggggttcca gggcctgtct                                                   20


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sgRNA target site sequences CTNNB1

<400>  69
ctggactctg gaatccattc                                                   20


<210>  70
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site2 Forward Primer

<400>  70
tggcccttca agttactgca                                                   20


<210>  71
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site2 Reverse Primer

<400>  71
agcacatgac agttaaggtt tgt                                               23


<210>  72
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site3 Forward Primer

<400>  72
aaacgcccat gcaattagtc                                                   20


<210>  73
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Site3 Reverse Primer

<400>  73
agcccctgtc taggaaaagc                                                   20


<210>  74
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTNNB1 Forward Primer

<400>  74
caatgggtca tatcacagat tctt                                              24


<210>  75
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CTNNB1 Reverse Primer

<400>  75
ccagctactt gttcttgagt gaa                                               23


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFR Forward Primer

<400>  76
tcatgcgtct tcacctggaa                                                   20


<210>  77
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EGFR Reverse Primer

<400>  77
cgcacacaca tatccccatg                                                   20


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9 Forward Primer

<400>  78
cactagcagg gacaaggtgg                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCSK9 Reverse Primer

<400>  79
attcagctca gatggggtgg                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FANCF Forward Primer

<400>  80
cgctgggaga ttgacatgca                                                   20


<210>  81
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FANCF Reverse Primer

<400>  81
ctcttgcctc cactggttgt                                                   20


<210>  82
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC Forward Primer

<400>  82
acctacccca tccccagaag                                                   20


<210>  83
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC Reverse Primer

<400>  83
tccctaaacc ccactcccag                                                   20


<210>  84
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M Forward Primer

<400>  84
tgggtttcat ccatccgaca t                                                 21


<210>  85
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M Reverse Primer

<400>  85
atgggatggg actcattcag g                                                 21


<210>  86
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic crRNA Sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N denotes the 20 nt target specific spacer sequence

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  2'OMe  (2'-O-Methyl nucleotides) and phosphorothioate modified 
       residue

<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  2'OMe  (2'-O-Methyl nucleotides) and phosphorothioate modified 
       residue

<400>  86
nnnnnnnnnn nnnnnnnnnn guuuuagagc uaugcuguuu ug                          42


<210>  87
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic 1xMS2 tracrRNA-Aptamer Sequence


<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  2'OMe (m) and phosphorothioate (*) modified residues

<220>
<221>  misc_feature
<222>  (97)..(98)
<223>  2'OMe and phosphorothioate modified residues

<400>  87
aacagcauag caaguuaaaa uaaggcuagu ccguuaucaa cuugaaaaag uggcaccgag       60

ucggugcgcg cacaugagga ucacccaugu gcuuuuuuu                              99


<210>  88
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic 2xMS2 tracrRNA-Aptamer Sequence


<220>
<221>  misc_feature
<222>  (139)..(140)
<223>  2'OMe and phosphorothioate modified residue

<220>
<221>  misc_feature
<222>  (139)..(140)
<223>  2'OMe and phosphorothioate modified residues

<400>  88
aacagcauag caaguuaaaa uaaggcuagu ccguuaucaa cuugaaaaag uggcaccgag       60

ucggugcggg agcacaugag gaucacccau gugccacgag cgacaugagg aucacccaug      120

ucgcucgugu ucccuuuuuu u                                                141


<210>  89
<211>  167
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lentiviral sgRNA sequences


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  n's denote the 20 base target specific sequence

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  residues 1 and 2 are phosphothorothioate modified

<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  residues 1 and 2 are 2'OMe modified

<220>
<221>  misc_feature
<222>  (168)..(169)
<223>  residues 168 and 169 are phosphorothioate modified

<220>
<221>  misc_feature
<222>  (168)..(169)
<223>  residues 168 and 169 are 2'OMe modified

<400>  89
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugcggga gcacaugagg aucacccaug      120

ugccacgagc gacaugagga ucacccaugu cgcucguguu cccuuuu                    167


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_1 sgRNA guide sequence

<400>  90
cacagcccaa gatagttaag                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_2 sgRNA guide sequence

<400>  91
acagcccaag atagttaagt                                                   20


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_3 sgRNA guide sequence

<400>  92
ttaccccact taactatctt                                                   20


<210>  93
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_4 sgRNA guide sequence

<400>  93
cttaccccac ttaactatct                                                   20


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_5 sgRNA guide sequence

<400>  94
actcacgctg gatagcctcc                                                   20


<210>  95
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_6 sgRNA guide sequence

<400>  95
ttggagtacc tgaggaatat                                                   20


<210>  96
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_7 sgRNA guide sequence

<400>  96
tcgatctatg aaaaagacag                                                   20


<210>  97
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  B2M_8 sgRNA guide sequence

<400>  97
aacctgaaaa gaaaagaaaa                                                   20


<210>  98
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_1 sgRNA guide sequence

<400>  98
gtacaggtaa gagcaacgcc                                                   20


<210>  99
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_2 sgRNA guide sequence

<400>  99
ctcctcctac agatacaaac                                                   20


<210>  100
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_3 sgRNA guide sequence

<400>  100
cagatacaaa ctggactctc                                                   20


<210>  101
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_4 sgRNA guide sequence

<400>  101
ctcttacctg taccataacc                                                   20


<210>  102
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_5 sgRNA guide sequence

<400>  102
gtatctgtag gaggagaagt                                                   20


<210>  103
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_6 sgRNA guide sequence

<400>  103
tgtatctgta ggaggagaag                                                   20


<210>  104
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD52_7 sgRNA guide sequence

<400>  104
gtccagtttg tatctgtagg                                                   20


<210>  105
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_1 sgRNA guide sequence

<400>  105
aacaaatgtg tcacaaagta                                                   20


<210>  106
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_2 sgRNA guide sequence

<400>  106
cttcttcccc agcccaggta                                                   20


<210>  107
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_3 sgRNA guide sequence

<400>  107
ttcttcccca gcccaggtaa                                                   20


<210>  108
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_4 sgRNA guide sequence

<400>  108
agcccaggta agggcagctt                                                   20


<210>  109
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_5 sgRNA guide sequence

<400>  109
tttcaaaacc tgtcagtgat                                                   20


<210>  110
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_6 sgRNA guide sequence

<400>  110
ttcaaaacct gtcagtgatt                                                   20


<210>  111
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_7 sgRNA guide sequence

<400>  111
ccgaatcctc ctcctgaaag                                                   20


<210>  112
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_8 sgRNA guide sequence

<400>  112
cttacctggg ctggggaaga                                                   20


<210>  113
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRAC_9 sgRNA guide sequence

<400>  113
ttcgtatctg taaaaccaag                                                   20


<210>  114
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_1 sgRNA guide sequence

<400>  114
ccacacccaa aaggccacac                                                   20


<210>  115
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_2 sgRNA guide sequence

<400>  115
cccaccagct cagctccacg                                                   20


<210>  116
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_3 sgRNA guide sequence

<400>  116
cgctgtcaag tccagttcta                                                   20


<210>  117
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_4 sgRNA guide sequence

<400>  117
gctgtcaagt ccagttctac                                                   20


<210>  118
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_5 sgRNA guide sequence

<400>  118
agtccagttc tacgggctct                                                   20


<210>  119
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_6 sgRNA guide sequence

<400>  119
cacccagatc gtcagcgccg                                                   20


<210>  120
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_7 sgRNA guide sequence

<400>  120
acctgctcta ccccaggcct                                                   20


<210>  121
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1/2_8 sgRNA guide sequence

<400>  121
ccactcacct gctctacccc                                                   20


<210>  122
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_1 sgRNA guide sequence

<400>  122
cacggacccg cagcccctca                                                   20


<210>  123
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_2 sgRNA guide sequence

<400>  123
gcgggggttc tgccagaagg                                                   20


<210>  124
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_3 sgRNA guide sequence

<400>  124
gttgcggggg ttctgccaga                                                   20


<210>  125
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_4 sgRNA guide sequence

<400>  125
atgacgagtg gacccaggat                                                   20


<210>  126
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_5 sgRNA guide sequence

<400>  126
tgacgagtgg acccaggata                                                   20


<210>  127
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_6 sgRNA guide sequence

<400>  127
acctgctcta ccccaggcct                                                   20


<210>  128
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_7 sgRNA guide sequence

<400>  128
ccaacagtgt cctaccagca                                                   20


<210>  129
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_8 sgRNA guide sequence

<400>  129
caacagtgtc ctaccagcaa                                                   20


<210>  130
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  	TRBC1_9 sgRNA guide sequence

<400>  130
aacagtgtcc taccagcaag                                                   20


<210>  131
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_10 sgRNA guide sequence

<400>  131
gtctgaaaga aagcagggag                                                   20


<210>  132
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_11 sgRNA guide sequence

<400>  132
ccacagtctg aaagaaagca                                                   20


<210>  133
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_12 sgRNA guide sequence

<400>  133
gccacagtct gaaagaaagc                                                   20


<210>  134
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_13 sgRNA guide sequence

<400>  134
gacactgttg gcacggagga                                                   20


<210>  135
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_14 sgRNA guide sequence

<400>  135
gtaggacact gttggcacgg                                                   20


<210>  136
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_15 sgRNA guide sequence

<400>  136
taccatggcc atcaacacaa                                                   20


<210>  137
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC1_16 sgRNA guide sequence

<400>  137
ttaccatggc catcaacaca                                                   20


<210>  138
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_1 sgRNA guide sequence

<400>  138
ccagctcagc tccacgtggt                                                   20


<210>  139
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_2 sgRNA guide sequence

<400>  139
cacagacccg cagcccctca                                                   20


<210>  140
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_3 sgRNA guide sequence

<400>  140
gcgggggttc tgccagaagg                                                   20


<210>  141
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_4 sgRNA guide sequence

<400>  141
gttgcggggg ttctgccaga                                                   20


<210>  142
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_5 sgRNA guide sequence

<400>  142
atgacgagtg gacccaggat                                                   20


<210>  143
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_6 sgRNA guide sequence

<400>  143
tgacgagtgg acccaggata                                                   20


<210>  144
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_7 sgRNA guide sequence

<400>  144
acctgctcta ccccaggcct                                                   20


<210>  145
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_8 sgRNA guide sequence

<400>  145
tcaacagagt cttaccagca                                                   20


<210>  146
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_9 sgRNA guide sequence

<400>  146
caacagagtc ttaccagcaa                                                   20


<210>  147
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_10 sgRNA guide sequence

<400>  147
aacagagtct taccagcaag                                                   20


<210>  148
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_11 sgRNA guide sequence

<400>  148
cacagtctga aagaaaacag                                                   20


<210>  149
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_12 sgRNA guide sequence

<400>  149
ccacagtctg aaagaaaaca                                                   20


<210>  150
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRBC2_13 sgRNA guide sequence

<400>  150
gccacagtct gaaagaaaac                                                   20


<210>  151
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_1 sgRNA guide sequence

<400>  151
tccaggcatg cagatcccac                                                   20


<210>  152
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_2 sgRNA guide sequence

<400>  152
tgcagatccc acaggcgccc                                                   20


<210>  153
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_3 sgRNA guide sequence

<400>  153
cgactggcca gggcgcctgt                                                   20


<210>  154
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_4 sgRNA guide sequence

<400>  154
acgactggcc agggcgcctg                                                   20


<210>  155
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_5 sgRNA guide sequence

<400>  155
accgcccaga cgactggcca                                                   20


<210>  156
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_6 sgRNA guide sequence

<400>  156
caccgcccag acgactggcc                                                   20


<210>  157
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_7 sgRNA guide sequence

<400>  157
tgtagcaccg cccagacgac                                                   20


<210>  158
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_8 sgRNA guide sequence

<400>  158
gggcggtgct acaactgggc                                                   20


<210>  159
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_9 sgRNA guide sequence

<400>  159
cggtgctaca actgggctgg                                                   20


<210>  160
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_10 sgRNA guide sequence

<400>  160
ctacaactgg gctggcggcc                                                   20


<210>  161
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_11 sgRNA guide sequence

<400>  161
cacctaccta agaaccatcc                                                   20


<210>  162
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  =PDCD1_12 sgRNA guide sequence

<400>  162
ggggttccag ggcctgtctg                                                   20


<210>  163
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_13 sgRNA guide sequence

<400>  163
gggggttcca gggcctgtct                                                   20


<210>  164
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_14 sgRNA guide sequence

<400>  164
ggggggttcc agggcctgtc                                                   20


<210>  165
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_15 sgRNA guide sequence

<400>  165
cagcaaccag acggacaagc                                                   20


<210>  166
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_16 sgRNA guide sequence

<400>  166
cccgaggacc gcagccagcc                                                   20


<210>  167
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_17 sgRNA guide sequence

<400>  167
ggaccgcagc cagcccggcc                                                   20


<210>  168
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_18 sgRNA guide sequence

<400>  168
cgtgtcacac aactgcccaa                                                   20


<210>  169
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_19 sgRNA guide sequence

<400>  169
gtgtcacaca actgcccaac                                                   20


<210>  170
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_20 sgRNA guide sequence

<400>  170
cgcagatcaa agagagcctg                                                   20


<210>  171
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_21 sgRNA guide sequence

<400>  171
gcagatcaaa gagagcctgc                                                   20


<210>  172
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_22 sgRNA guide sequence

<400>  172
agccggccag ttccaaaccc                                                   20


<210>  173
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_23 sgRNA guide sequence

<400>  173
cggccagttc caaaccctgg                                                   20


<210>  174
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_24 sgRNA guide sequence

<400>  174
cagttccaaa ccctggtggt                                                   20


<210>  175
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_25 sgRNA guide sequence

<400>  175
ggacccagac tagcagcacc                                                   20


<210>  176
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_26 sgRNA guide sequence

<400>  176
cacctaccta agaaccatcc                                                   20


<210>  177
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_27 sgRNA guide sequence

<400>  177
ggagtctgag agatggagag                                                   20


<210>  178
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_28 sgRNA guide sequence

<400>  178
tctggaaggg cacaaaggtc                                                   20


<210>  179
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_29 sgRNA guide sequence

<400>  179
ttctctctgg aagggcacaa                                                   20


<210>  180
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_30 sgRNA guide sequence

<400>  180
tgacgttacc tcgtgcggcc                                                   20


<210>  181
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_31 sgRNA guide sequence

<400>  181
tccctgcaga gaaacacact                                                   20


<210>  182
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_32 sgRNA guide sequence

<400>  182
gagactcacc aggggctggc                                                   20


<210>  183
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_33 sgRNA guide sequence

<400>  183
tctttgagga gaaagggaga                                                   20


<210>  184
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDCD1_34 sgRNA guide sequence

<400>  184
ttctttgagg agaaagggag                                                   20


<210>  185
<211>  99
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  1x MS2_3? tracrRNA (F-5)


<220>
<221>  misc_feature
<222>  (77)..(77)
<223>  x = 2AdP (2-aminopurine)

<220>
<221>  misc_feature
<222>  (97)..(97)
<223>  U is modified with 2'OMe and phosphorothioate

<220>
<221>  misc_feature
<222>  (98)..(98)
<223>  U is modified with 2'OMe and phosphorothioate

<400>  185
aacagcauag caaguuaaaa uaaggcuagu ccguuaucaa cuugaaaaag uggcaccgag       60

ucggugcgcg gcccggngga ucaccacggg ccuuuuuuu                              99


<210>  186
<211>  1981
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Protein sequence of RNA scaffold mediated recruitment system (2 
       xUGI)


<220>
<221>  MISC_FEATURE
<222>  (1)..(7)
<223>  Nuclear Localization Signal (NLS)

<220>
<221>  MISC_FEATURE
<222>  (8)..(235)
<223>  APOBEC1

<220>
<221>  MISC_FEATURE
<222>  (236)..(261)
<223>  Linker

<220>
<221>  MISC_FEATURE
<222>  (262)..(378)
<223>  MCP

<220>
<221>  MISC_FEATURE
<222>  (382)..(399)
<223>  T2A peptide

<220>
<221>  MISC_FEATURE
<222>  (402)..(408)
<223>  Nuclear Localization Signal (NLS)

<220>
<221>  MISC_FEATURE
<222>  (409)..(1775)
<223>  nCAS9D10A

<220>
<221>  MISC_FEATURE
<222>  (1786)..(1868)
<223>  UGI

<220>
<221>  MISC_FEATURE
<222>  (1879)..(1961)
<223>  UGI

<220>
<221>  MISC_FEATURE
<222>  (1976)..(1982)
<223>  Nuclear Localization Signal (NLS)

<400>  186

Lys Lys Lys Arg Lys Val Met Ser Ser Glu Thr Gly Pro Val Ala Val 
1               5                   10                  15      


Asp Pro Thr Leu Arg Arg Arg Ile Glu Pro His Glu Phe Glu Val Phe 
            20                  25                  30          


Phe Asp Pro Arg Glu Leu Arg Lys Glu Thr Cys Leu Leu Tyr Glu Ile 
        35                  40                  45              


Asn Trp Gly Gly Arg His Ser Ile Trp Arg His Thr Ser Gln Asn Thr 
    50                  55                  60                  


Asn Lys His Val Glu Val Asn Phe Ile Glu Lys Phe Thr Thr Glu Arg 
65                  70                  75                  80  


Tyr Phe Cys Pro Asn Thr Arg Cys Ser Ile Thr Trp Phe Leu Ser Trp 
                85                  90                  95      


Ser Pro Cys Gly Glu Cys Ser Arg Ala Ile Thr Glu Phe Leu Ser Arg 
            100                 105                 110         


Tyr Pro His Val Thr Leu Phe Ile Tyr Ile Ala Arg Leu Tyr His His 
        115                 120                 125             


Ala Asp Pro Arg Asn Arg Gln Gly Leu Arg Asp Leu Ile Ser Ser Gly 
    130                 135                 140                 


Val Thr Ile Gln Ile Met Thr Glu Gln Glu Ser Gly Tyr Cys Trp Arg 
145                 150                 155                 160 


Asn Phe Val Asn Tyr Ser Pro Ser Asn Glu Ala His Trp Pro Arg Tyr 
                165                 170                 175     


Pro His Leu Trp Val Arg Leu Tyr Val Leu Glu Leu Tyr Cys Ile Ile 
            180                 185                 190         


Leu Gly Leu Pro Pro Cys Leu Asn Ile Leu Arg Arg Lys Gln Pro Gln 
        195                 200                 205             


Leu Thr Phe Phe Thr Ile Ala Leu Gln Ser Cys His Tyr Gln Arg Leu 
    210                 215                 220                 


Pro Pro His Ile Leu Trp Ala Thr Gly Leu Lys Glu Leu Lys Thr Pro 
225                 230                 235                 240 


Leu Gly Asp Thr Thr His Thr Ser Pro Pro Cys Pro Ala Pro Glu Leu 
                245                 250                 255     


Leu Gly Gly Pro Met Ala Ser Asn Phe Thr Gln Phe Val Leu Val Asp 
            260                 265                 270         


Asn Gly Gly Thr Gly Asp Val Thr Val Ala Pro Ser Asn Phe Ala Asn 
        275                 280                 285             


Gly Ile Ala Glu Trp Ile Ser Ser Asn Ser Arg Ser Gln Ala Tyr Lys 
    290                 295                 300                 


Val Thr Cys Ser Val Arg Gln Ser Ser Ala Gln Asn Arg Lys Tyr Thr 
305                 310                 315                 320 


Ile Lys Val Glu Val Pro Lys Gly Ala Trp Arg Ser Tyr Leu Asn Met 
                325                 330                 335     


Glu Leu Thr Ile Pro Ile Phe Ala Thr Asn Ser Asp Cys Glu Leu Ile 
            340                 345                 350         


Val Lys Ala Met Gln Gly Leu Leu Lys Asp Gly Asn Pro Ile Pro Ser 
        355                 360                 365             


Ala Ile Ala Ala Asn Ser Gly Ile Tyr Gly Ser Gly Glu Gly Arg Gly 
    370                 375                 380                 


Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Gly Thr 
385                 390                 395                 400 


Pro Lys Lys Lys Arg Lys Val Asp Lys Lys Tyr Ser Ile Gly Leu Ala 
                405                 410                 415     


Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys 
            420                 425                 430         


Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser 
        435                 440                 445             


Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr 
    450                 455                 460                 


Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg 
465                 470                 475                 480 


Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met 
                485                 490                 495     


Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu 
            500                 505                 510         


Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile 
        515                 520                 525             


Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu 
    530                 535                 540                 


Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile 
545                 550                 555                 560 


Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile 
                565                 570                 575     


Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile 
            580                 585                 590         


Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn 
        595                 600                 605             


Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys 
    610                 615                 620                 


Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys 
625                 630                 635                 640 


Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro 
                645                 650                 655     


Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu 
            660                 665                 670         


Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile 
        675                 680                 685             


Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp 
    690                 695                 700                 


Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys 
705                 710                 715                 720 


Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln 
                725                 730                 735     


Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys 
            740                 745                 750         


Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr 
        755                 760                 765             


Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro 
    770                 775                 780                 


Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn 
785                 790                 795                 800 


Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile 
                805                 810                 815     


Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln 
            820                 825                 830         


Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys 
        835                 840                 845             


Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly 
    850                 855                 860                 


Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile Thr 
865                 870                 875                 880 


Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala Gln Ser 
                885                 890                 895     


Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys 
            900                 905                 910         


Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn 
        915                 920                 925             


Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys Pro Ala 
    930                 935                 940                 


Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu Phe Lys 
945                 950                 955                 960 


Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys 
                965                 970                 975     


Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu Asp Arg 
            980                 985                 990         


Phe Asn Ala Ser Leu Gly Thr Tyr  His Asp Leu Leu Lys  Ile Ile Lys 
        995                 1000                 1005             


Asp Lys  Asp Phe Leu Asp Asn  Glu Glu Asn Glu Asp  Ile Leu Glu 
    1010                 1015                 1020             


Asp Ile  Val Leu Thr Leu Thr  Leu Phe Glu Asp Arg  Glu Met Ile 
    1025                 1030                 1035             


Glu Glu  Arg Leu Lys Thr Tyr  Ala His Leu Phe Asp  Asp Lys Val 
    1040                 1045                 1050             


Met Lys  Gln Leu Lys Arg Arg  Arg Tyr Thr Gly Trp  Gly Arg Leu 
    1055                 1060                 1065             


Ser Arg  Lys Leu Ile Asn Gly  Ile Arg Asp Lys Gln  Ser Gly Lys 
    1070                 1075                 1080             


Thr Ile  Leu Asp Phe Leu Lys  Ser Asp Gly Phe Ala  Asn Arg Asn 
    1085                 1090                 1095             


Phe Met  Gln Leu Ile His Asp  Asp Ser Leu Thr Phe  Lys Glu Asp 
    1100                 1105                 1110             


Ile Gln  Lys Ala Gln Val Ser  Gly Gln Gly Asp Ser  Leu His Glu 
    1115                 1120                 1125             


His Ile  Ala Asn Leu Ala Gly  Ser Pro Ala Ile Lys  Lys Gly Ile 
    1130                 1135                 1140             


Leu Gln  Thr Val Lys Val Val  Asp Glu Leu Val Lys  Val Met Gly 
    1145                 1150                 1155             


Arg His  Lys Pro Glu Asn Ile  Val Ile Glu Met Ala  Arg Glu Asn 
    1160                 1165                 1170             


Gln Thr  Thr Gln Lys Gly Gln  Lys Asn Ser Arg Glu  Arg Met Lys 
    1175                 1180                 1185             


Arg Ile  Glu Glu Gly Ile Lys  Glu Leu Gly Ser Gln  Ile Leu Lys 
    1190                 1195                 1200             


Glu His  Pro Val Glu Asn Thr  Gln Leu Gln Asn Glu  Lys Leu Tyr 
    1205                 1210                 1215             


Leu Tyr  Tyr Leu Gln Asn Gly  Arg Asp Met Tyr Val  Asp Gln Glu 
    1220                 1225                 1230             


Leu Asp  Ile Asn Arg Leu Ser  Asp Tyr Asp Val Asp  His Ile Val 
    1235                 1240                 1245             


Pro Gln  Ser Phe Leu Lys Asp  Asp Ser Ile Asp Asn  Lys Val Leu 
    1250                 1255                 1260             


Thr Arg  Ser Asp Lys Asn Arg  Gly Lys Ser Asp Asn  Val Pro Ser 
    1265                 1270                 1275             


Glu Glu  Val Val Lys Lys Met  Lys Asn Tyr Trp Arg  Gln Leu Leu 
    1280                 1285                 1290             


Asn Ala  Lys Leu Ile Thr Gln  Arg Lys Phe Asp Asn  Leu Thr Lys 
    1295                 1300                 1305             


Ala Glu  Arg Gly Gly Leu Ser  Glu Leu Asp Lys Ala  Gly Phe Ile 
    1310                 1315                 1320             


Lys Arg  Gln Leu Val Glu Thr  Arg Gln Ile Thr Lys  His Val Ala 
    1325                 1330                 1335             


Gln Ile  Leu Asp Ser Arg Met  Asn Thr Lys Tyr Asp  Glu Asn Asp 
    1340                 1345                 1350             


Lys Leu  Ile Arg Glu Val Lys  Val Ile Thr Leu Lys  Ser Lys Leu 
    1355                 1360                 1365             


Val Ser  Asp Phe Arg Lys Asp  Phe Gln Phe Tyr Lys  Val Arg Glu 
    1370                 1375                 1380             


Ile Asn  Asn Tyr His His Ala  His Asp Ala Tyr Leu  Asn Ala Val 
    1385                 1390                 1395             


Val Gly  Thr Ala Leu Ile Lys  Lys Tyr Pro Lys Leu  Glu Ser Glu 
    1400                 1405                 1410             


Phe Val  Tyr Gly Asp Tyr Lys  Val Tyr Asp Val Arg  Lys Met Ile 
    1415                 1420                 1425             


Ala Lys  Ser Glu Gln Glu Ile  Gly Lys Ala Thr Ala  Lys Tyr Phe 
    1430                 1435                 1440             


Phe Tyr  Ser Asn Ile Met Asn  Phe Phe Lys Thr Glu  Ile Thr Leu 
    1445                 1450                 1455             


Ala Asn  Gly Glu Ile Arg Lys  Arg Pro Leu Ile Glu  Thr Asn Gly 
    1460                 1465                 1470             


Glu Thr  Gly Glu Ile Val Trp  Asp Lys Gly Arg Asp  Phe Ala Thr 
    1475                 1480                 1485             


Val Arg  Lys Val Leu Ser Met  Pro Gln Val Asn Ile  Val Lys Lys 
    1490                 1495                 1500             


Thr Glu  Val Gln Thr Gly Gly  Phe Ser Lys Glu Ser  Ile Leu Pro 
    1505                 1510                 1515             


Lys Arg  Asn Ser Asp Lys Leu  Ile Ala Arg Lys Lys  Asp Trp Asp 
    1520                 1525                 1530             


Pro Lys  Lys Tyr Gly Gly Phe  Asp Ser Pro Thr Val  Ala Tyr Ser 
    1535                 1540                 1545             


Val Leu  Val Val Ala Lys Val  Glu Lys Gly Lys Ser  Lys Lys Leu 
    1550                 1555                 1560             


Lys Ser  Val Lys Glu Leu Leu  Gly Ile Thr Ile Met  Glu Arg Ser 
    1565                 1570                 1575             


Ser Phe  Glu Lys Asn Pro Ile  Asp Phe Leu Glu Ala  Lys Gly Tyr 
    1580                 1585                 1590             


Lys Glu  Val Lys Lys Asp Leu  Ile Ile Lys Leu Pro  Lys Tyr Ser 
    1595                 1600                 1605             


Leu Phe  Glu Leu Glu Asn Gly  Arg Lys Arg Met Leu  Ala Ser Ala 
    1610                 1615                 1620             


Gly Glu  Leu Gln Lys Gly Asn  Glu Leu Ala Leu Pro  Ser Lys Tyr 
    1625                 1630                 1635             


Val Asn  Phe Leu Tyr Leu Ala  Ser His Tyr Glu Lys  Leu Lys Gly 
    1640                 1645                 1650             


Ser Pro  Glu Asp Asn Glu Gln  Lys Gln Leu Phe Val  Glu Gln His 
    1655                 1660                 1665             


Lys His  Tyr Leu Asp Glu Ile  Ile Glu Gln Ile Ser  Glu Phe Ser 
    1670                 1675                 1680             


Lys Arg  Val Ile Leu Ala Asp  Ala Asn Leu Asp Lys  Val Leu Ser 
    1685                 1690                 1695             


Ala Tyr  Asn Lys His Arg Asp  Lys Pro Ile Arg Glu  Gln Ala Glu 
    1700                 1705                 1710             


Asn Ile  Ile His Leu Phe Thr  Leu Thr Asn Leu Gly  Ala Pro Ala 
    1715                 1720                 1725             


Ala Phe  Lys Tyr Phe Asp Thr  Thr Ile Asp Arg Lys  Arg Tyr Thr 
    1730                 1735                 1740             


Ser Thr  Lys Glu Val Leu Asp  Ala Thr Leu Ile His  Gln Ser Ile 
    1745                 1750                 1755             


Thr Gly  Leu Tyr Glu Thr Arg  Ile Asp Leu Ser Gln  Leu Gly Gly 
    1760                 1765                 1770             


Asp Ser  Gly Gly Ser Gly Gly  Ser Gly Gly Ser Thr  Asn Leu Ser 
    1775                 1780                 1785             


Asp Ile  Ile Glu Lys Glu Thr  Gly Lys Gln Leu Val  Ile Gln Glu 
    1790                 1795                 1800             


Ser Ile  Leu Met Leu Pro Glu  Glu Val Glu Glu Val  Ile Gly Asn 
    1805                 1810                 1815             


Lys Pro  Glu Ser Asp Ile Leu  Val His Thr Ala Tyr  Asp Glu Ser 
    1820                 1825                 1830             


Thr Asp  Glu Asn Val Met Leu  Leu Thr Ser Asp Ala  Pro Glu Tyr 
    1835                 1840                 1845             


Lys Pro  Trp Ala Leu Val Ile  Gln Asp Ser Asn Gly  Glu Asn Lys 
    1850                 1855                 1860             


Ile Lys  Met Leu Ser Gly Gly  Ser Gly Gly Ser Gly  Gly Ser Thr 
    1865                 1870                 1875             


Asn Leu  Ser Asp Ile Ile Glu  Lys Glu Thr Gly Lys  Gln Leu Val 
    1880                 1885                 1890             


Ile Gln  Glu Ser Ile Leu Met  Leu Pro Glu Glu Val  Glu Glu Val 
    1895                 1900                 1905             


Ile Gly  Asn Lys Pro Glu Ser  Asp Ile Leu Val His  Thr Ala Tyr 
    1910                 1915                 1920             


Asp Glu  Ser Thr Asp Glu Asn  Val Met Leu Leu Thr  Ser Asp Ala 
    1925                 1930                 1935             


Pro Glu  Tyr Lys Pro Trp Ala  Leu Val Ile Gln Asp  Ser Asn Gly 
    1940                 1945                 1950             


Glu Asn  Lys Ile Lys Met Leu  Ser Gly Gly Ser Lys  Arg Thr Ala 
    1955                 1960                 1965             


Asp Gly  Ser Glu Phe Glu Pro  Lys Lys Lys Arg Lys  Val 
    1970                 1975                 1980     


