                         SEQUENCE LISTING

<110>  THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
       Pardi, Norbert
       Weissman, Drew
 
<120>  Nucleoside-modified RNA for Inducing an Immune Response Against 
       SARS-CoV-2

<130>  046483-6209-00WO

<150>  US 63/049,295
<151>  2020-07-08

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Full-length WT S protein DNA sequence

<400>  1
atgttcgtgt tcctggtgct gctgcccctg gtgtcctccc agtgcgtgaa cctgaccacc       60

cgcacccagc tgccccccgc ctacaccaac tccttcaccc gcggcgtgta ctaccccgac      120

aaggtgttcc gctcctccgt gctgcactcc acccaggacc tgttcctgcc cttcttctcc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca acggcaccaa gcggttcgac      240

aaccccgtgc tgcccttcaa cgacggcgtg tacttcgcct ccaccgagaa gtccaacatc      300

atccgcggct ggatcttcgg caccaccctg gactccaaga cccagtccct gctgatcgtg      360

aacaacgcca ccaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaag tcctggatgg agtccgagtt ccgcgtgtac      480

tcctccgcca acaactgcac cttcgagtac gtgtcccagc ccttcctgat ggacctggag      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct actccaagca cacccccatc aacctggtgc gcgacctgcc ccagggcttc      660

tccgccctgg agcccctggt ggacctgccc atcggcatca acatcacccg cttccagacc      720

ctgctggccc tgcaccgctc ctacctgacc cccggcgact cctcctccgg ctggaccgcc      780

ggcgccgccg cctactacgt gggctacctg cagccccgca ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggac tgcgccctgg accccctgtc cgagaccaag      900

tgcaccctga agtccttcac cgtggagaag ggcatctacc agacctccaa cttccgcgtg      960

cagcccaccg agtccatcgt gcgcttcccc aacatcacca acctgtgccc cttcggcgag     1020

gtgttcaacg ccacccgctt cgcctccgtg tacgcctgga accgcaagcg catctccaac     1080

tgcgtggccg actactccgt gctgtacaac tccgcctcct tctccacctt caagtgctac     1140

ggcgtgtccc ccaccaagct gaacgacctg tgcttcacca acgtgtacgc cgactccttc     1200

gtgatccgcg gcgacgaggt gcgccagatc gcccccggcc agaccggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgcgtga tcgcctggaa ctccaacaac     1320

ctggactcca aggtgggcgg caactacaac tacctgtacc gcctgttccg caagtccaac     1380

ctgaagccct tcgagcgcga catctccacc gagatctacc aggccggctc caccccctgc     1440

aacggcgtgg agggcttcaa ctgctacttc cccctgcagt cctacggctt ccagcccacc     1500

aacggcgtgg gctaccagcc ctaccgcgtg gtggtgctgt ccttcgagct gctgcacgcc     1560

cccgccaccg tgtgcggccc caagaagtcc accaacctgg tgaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgaccg agtccaacaa gaagttcctg     1680

cccttccagc agttcggccg cgacatcgcc gacaccaccg acgccgtgcg cgacccccag     1740

accctggaga tcctggacat caccccctgc tccttcggcg gcgtgtccgt gatcaccccc     1800

ggcaccaaca cctccaacca ggtggccgtg ctgtaccagg acgtgaactg caccgaggtg     1860

cccgtggcca tccacgccga ccagctgacc cccacctggc gcgtgtactc caccggctcc     1920

aacgtgttcc agacccgcgc cggctgcctg atcggcgccg agcacgtgaa caactcctac     1980

gagtgcgaca tccccatcgg cgccggcatc tgcgcctcct accagaccca gaccaactcc     2040

ccccgccgcg cccgctccgt ggcctcccag tccatcatcg cctacaccat gtccctgggc     2100

gccgagaact ccgtggccta ctccaacaac tccatcgcca tccccaccaa cttcaccatc     2160

tccgtgacca ccgagatcct gcccgtgtcc atgaccaaga cctccgtgga ctgcaccatg     2220

tacatctgcg gcgactccac cgagtgctcc aacctgctgc tgcagtacgg ctccttctgc     2280

acccagctga accgcgccct gaccggcatc gccgtggagc aggacaagaa cacccaggag     2340

gtgttcgccc aggtgaagca gatctacaag acccccccca tcaaggactt cggcggcttc     2400

aacttctccc agatcctgcc cgacccctcc aagccctcca agcggtcctt catcgaggac     2460

ctgctgttca acaaggtgac cctggccgac gccggcttca tcaagcagta cggcgactgc     2520

ctgggcgaca tcgccgcccg cgacctgatc tgcgcccaga agttcaacgg cctgaccgtg     2580

ctgccccccc tgctgaccga cgagatgatc gcccagtaca cctccgccct gctggccggc     2640

accatcacct ccggctggac cttcggcgcc ggcgccgccc tgcagatccc cttcgccatg     2700

cagatggcct accgcttcaa cggcatcggc gtgacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caactccgcc atcggcaaga tccaggactc cctgtcctcc     2820

accgcctccg ccctgggcaa gctgcaggac gtggtgaacc agaacgccca ggccctgaac     2880

accctggtga agcagctgtc ctccaacttc ggcgccatct cctccgtgct gaacgacatc     2940

ctgtcccgcc tggacaaggt ggaggccgag gtgcagatcg accgcctgat caccggccgc     3000

ctgcagtccc tgcagaccta cgtgacccag cagctgatcc gcgccgccga gatccgcgcc     3060

tccgccaacc tggccgccac caagatgtcc gagtgcgtgc tgggccagtc caagcgcgtg     3120

gacttctgcg gcaagggcta ccacctgatg tccttccccc agtccgcccc ccacggcgtg     3180

gtgttcctgc acgtgaccta cgtgcccgcc caggagaaga acttcaccac cgcccccgcc     3240

atctgccacg acggcaaggc ccacttcccc cgcgagggcg tgttcgtgtc caacggcacc     3300

cactggttcg tgacccagcg caacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtccg gcaactgcga cgtggtgatc ggcatcgtga acaacaccgt gtacgacccc     3420

ctgcagcccg agctggactc cttcaaggag gagctggaca agtacttcaa gaaccacacc     3480

tcccccgacg tggacctggg cgacatctcc ggcatcaacg cctccgtggt gaacatccag     3540

aaggagatcg accgcctgaa cgaggtggcc aagaacctga acgagtccct gatcgacctg     3600

caggagctgg gcaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttc     3660

atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgcat gacctcctgc     3720

tgctcctgcc tgaagggctg ctgctcctgc ggctcctgct gcaagttcga cgaggacgac     3780

tccgagcccg tgctgaaggg cgtgaagctg cactacacct aa                        3822


<210>  2
<211>  4233
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Full-length WT S protein mRNA-coding DNA 
       sequence

<400>  2
agcataaaag tctcaacaca acatatacaa aacaaacgaa tctcaagcaa tcaagcattc       60

tacttctatt gcagcaattt aaatcatttc ttttaaagca aaagcaattt tctgaaaatt      120

ttcaccattt acgaacgata gcgctatgtt cgtgttcctg gtgctgctgc ccctggtgtc      180

ctcccagtgc gtgaacctga ccacccgcac ccagctgccc cccgcctaca ccaactcctt      240

cacccgcggc gtgtactacc ccgacaaggt gttccgctcc tccgtgctgc actccaccca      300

ggacctgttc ctgcccttct tctccaacgt gacctggttc cacgccatcc acgtgtccgg      360

caccaacggc accaagcggt tcgacaaccc cgtgctgccc ttcaacgacg gcgtgtactt      420

cgcctccacc gagaagtcca acatcatccg cggctggatc ttcggcacca ccctggactc      480

caagacccag tccctgctga tcgtgaacaa cgccaccaac gtggtgatca aggtgtgcga      540

gttccagttc tgcaacgacc ccttcctggg cgtgtactac cacaagaaca acaagtcctg      600

gatggagtcc gagttccgcg tgtactcctc cgccaacaac tgcaccttcg agtacgtgtc      660

ccagcccttc ctgatggacc tggagggcaa gcagggcaac ttcaagaacc tgcgcgagtt      720

cgtgttcaag aacatcgacg gctacttcaa gatctactcc aagcacaccc ccatcaacct      780

ggtgcgcgac ctgccccagg gcttctccgc cctggagccc ctggtggacc tgcccatcgg      840

catcaacatc acccgcttcc agaccctgct ggccctgcac cgctcctacc tgacccccgg      900

cgactcctcc tccggctgga ccgccggcgc cgccgcctac tacgtgggct acctgcagcc      960

ccgcaccttc ctgctgaagt acaacgagaa cggcaccatc accgacgccg tggactgcgc     1020

cctggacccc ctgtccgaga ccaagtgcac cctgaagtcc ttcaccgtgg agaagggcat     1080

ctaccagacc tccaacttcc gcgtgcagcc caccgagtcc atcgtgcgct tccccaacat     1140

caccaacctg tgccccttcg gcgaggtgtt caacgccacc cgcttcgcct ccgtgtacgc     1200

ctggaaccgc aagcgcatct ccaactgcgt ggccgactac tccgtgctgt acaactccgc     1260

ctccttctcc accttcaagt gctacggcgt gtcccccacc aagctgaacg acctgtgctt     1320

caccaacgtg tacgccgact ccttcgtgat ccgcggcgac gaggtgcgcc agatcgcccc     1380

cggccagacc ggcaagatcg ccgactacaa ctacaagctg cccgacgact tcaccggctg     1440

cgtgatcgcc tggaactcca acaacctgga ctccaaggtg ggcggcaact acaactacct     1500

gtaccgcctg ttccgcaagt ccaacctgaa gcccttcgag cgcgacatct ccaccgagat     1560

ctaccaggcc ggctccaccc cctgcaacgg cgtggagggc ttcaactgct acttccccct     1620

gcagtcctac ggcttccagc ccaccaacgg cgtgggctac cagccctacc gcgtggtggt     1680

gctgtccttc gagctgctgc acgcccccgc caccgtgtgc ggccccaaga agtccaccaa     1740

cctggtgaag aacaagtgcg tgaacttcaa cttcaacggc ctgaccggca ccggcgtgct     1800

gaccgagtcc aacaagaagt tcctgccctt ccagcagttc ggccgcgaca tcgccgacac     1860

caccgacgcc gtgcgcgacc cccagaccct ggagatcctg gacatcaccc cctgctcctt     1920

cggcggcgtg tccgtgatca cccccggcac caacacctcc aaccaggtgg ccgtgctgta     1980

ccaggacgtg aactgcaccg aggtgcccgt ggccatccac gccgaccagc tgacccccac     2040

ctggcgcgtg tactccaccg gctccaacgt gttccagacc cgcgccggct gcctgatcgg     2100

cgccgagcac gtgaacaact cctacgagtg cgacatcccc atcggcgccg gcatctgcgc     2160

ctcctaccag acccagacca actccccccg ccgcgcccgc tccgtggcct cccagtccat     2220

catcgcctac accatgtccc tgggcgccga gaactccgtg gcctactcca acaactccat     2280

cgccatcccc accaacttca ccatctccgt gaccaccgag atcctgcccg tgtccatgac     2340

caagacctcc gtggactgca ccatgtacat ctgcggcgac tccaccgagt gctccaacct     2400

gctgctgcag tacggctcct tctgcaccca gctgaaccgc gccctgaccg gcatcgccgt     2460

ggagcaggac aagaacaccc aggaggtgtt cgcccaggtg aagcagatct acaagacccc     2520

ccccatcaag gacttcggcg gcttcaactt ctcccagatc ctgcccgacc cctccaagcc     2580

ctccaagcgg tccttcatcg aggacctgct gttcaacaag gtgaccctgg ccgacgccgg     2640

cttcatcaag cagtacggcg actgcctggg cgacatcgcc gcccgcgacc tgatctgcgc     2700

ccagaagttc aacggcctga ccgtgctgcc ccccctgctg accgacgaga tgatcgccca     2760

gtacacctcc gccctgctgg ccggcaccat cacctccggc tggaccttcg gcgccggcgc     2820

cgccctgcag atccccttcg ccatgcagat ggcctaccgc ttcaacggca tcggcgtgac     2880

ccagaacgtg ctgtacgaga accagaagct gatcgccaac cagttcaact ccgccatcgg     2940

caagatccag gactccctgt cctccaccgc ctccgccctg ggcaagctgc aggacgtggt     3000

gaaccagaac gcccaggccc tgaacaccct ggtgaagcag ctgtcctcca acttcggcgc     3060

catctcctcc gtgctgaacg acatcctgtc ccgcctggac aaggtggagg ccgaggtgca     3120

gatcgaccgc ctgatcaccg gccgcctgca gtccctgcag acctacgtga cccagcagct     3180

gatccgcgcc gccgagatcc gcgcctccgc caacctggcc gccaccaaga tgtccgagtg     3240

cgtgctgggc cagtccaagc gcgtggactt ctgcggcaag ggctaccacc tgatgtcctt     3300

cccccagtcc gccccccacg gcgtggtgtt cctgcacgtg acctacgtgc ccgcccagga     3360

gaagaacttc accaccgccc ccgccatctg ccacgacggc aaggcccact tcccccgcga     3420

gggcgtgttc gtgtccaacg gcacccactg gttcgtgacc cagcgcaact tctacgagcc     3480

ccagatcatc accaccgaca acaccttcgt gtccggcaac tgcgacgtgg tgatcggcat     3540

cgtgaacaac accgtgtacg accccctgca gcccgagctg gactccttca aggaggagct     3600

ggacaagtac ttcaagaacc acacctcccc cgacgtggac ctgggcgaca tctccggcat     3660

caacgcctcc gtggtgaaca tccagaagga gatcgaccgc ctgaacgagg tggccaagaa     3720

cctgaacgag tccctgatcg acctgcagga gctgggcaag tacgagcagt acatcaagtg     3780

gccctggtac atctggctgg gcttcatcgc cggcctgatc gccatcgtga tggtgaccat     3840

catgctgtgc tgcatgacct cctgctgctc ctgcctgaag ggctgctgct cctgcggctc     3900

ctgctgcaag ttcgacgagg acgactccga gcccgtgctg aagggcgtga agctgcacta     3960

cacctaaact agtagtgact gactaggatc tggttaccac taaaccagcc tcaagaacac     4020

ccgaatggag tctctaagct acataatacc aacttacact tacaaaatgt tgtcccccaa     4080

aatgtagcca ttcgtatctg ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa     4140

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa                                  4233


<210>  3
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Full-length WT S protein AA sequence

<400>  3

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  4
<211>  3813
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Full-length S protein deleted furin 
       cleavage site DNA sequence

<400>  4
atgttcgtgt tcctggtgct gctgcccctg gtgtcctccc agtgcgtgaa cctgaccacc       60

cgcacccagc tgccccccgc ctacaccaac tccttcaccc gcggcgtgta ctaccccgac      120

aaggtgttcc gctcctccgt gctgcactcc acccaggacc tgttcctgcc cttcttctcc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca acggcaccaa gcggttcgac      240

aaccccgtgc tgcccttcaa cgacggcgtg tacttcgcct ccaccgagaa gtccaacatc      300

atccgcggct ggatcttcgg caccaccctg gactccaaga cccagtccct gctgatcgtg      360

aacaacgcca ccaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtgt actaccacaa gaacaacaag tcctggatgg agtccgagtt ccgcgtgtac      480

tcctccgcca acaactgcac cttcgagtac gtgtcccagc ccttcctgat ggacctggag      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct actccaagca cacccccatc aacctggtgc gcgacctgcc ccagggcttc      660

tccgccctgg agcccctggt ggacctgccc atcggcatca acatcacccg cttccagacc      720

ctgctggccc tgcaccgctc ctacctgacc cccggcgact cctcctccgg ctggaccgcc      780

ggcgccgccg cctactacgt gggctacctg cagccccgca ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggac tgcgccctgg accccctgtc cgagaccaag      900

tgcaccctga agtccttcac cgtggagaag ggcatctacc agacctccaa cttccgcgtg      960

cagcccaccg agtccatcgt gcgcttcccc aacatcacca acctgtgccc cttcggcgag     1020

gtgttcaacg ccacccgctt cgcctccgtg tacgcctgga accgcaagcg catctccaac     1080

tgcgtggccg actactccgt gctgtacaac tccgcctcct tctccacctt caagtgctac     1140

ggcgtgtccc ccaccaagct gaacgacctg tgcttcacca acgtgtacgc cgactccttc     1200

gtgatccgcg gcgacgaggt gcgccagatc gcccccggcc agaccggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgcgtga tcgcctggaa ctccaacaac     1320

ctggactcca aggtgggcgg caactacaac tacctgtacc gcctgttccg caagtccaac     1380

ctgaagccct tcgagcgcga catctccacc gagatctacc aggccggctc caccccctgc     1440

aacggcgtgg agggcttcaa ctgctacttc cccctgcagt cctacggctt ccagcccacc     1500

aacggcgtgg gctaccagcc ctaccgcgtg gtggtgctgt ccttcgagct gctgcacgcc     1560

cccgccaccg tgtgcggccc caagaagtcc accaacctgg tgaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgaccg agtccaacaa gaagttcctg     1680

cccttccagc agttcggccg cgacatcgcc gacaccaccg acgccgtgcg cgacccccag     1740

accctggaga tcctggacat caccccctgc tccttcggcg gcgtgtccgt gatcaccccc     1800

ggcaccaaca cctccaacca ggtggccgtg ctgtaccagg acgtgaactg caccgaggtg     1860

cccgtggcca tccacgccga ccagctgacc cccacctggc gcgtgtactc caccggctcc     1920

aacgtgttcc agacccgcgc cggctgcctg atcggcgccg agcacgtgaa caactcctac     1980

gagtgcgaca tccccatcgg cgccggcatc tgcgcctcct accagaccca gaccaactcc     2040

cccgcctccg tggcctccca gtccatcatc gcctacacca tgtccctggg cgccgagaac     2100

tccgtggcct actccaacaa ctccatcgcc atccccacca acttcaccat ctccgtgacc     2160

accgagatcc tgcccgtgtc catgaccaag acctccgtgg actgcaccat gtacatctgc     2220

ggcgactcca ccgagtgctc caacctgctg ctgcagtacg gctccttctg cacccagctg     2280

aaccgcgccc tgaccggcat cgccgtggag caggacaaga acacccagga ggtgttcgcc     2340

caggtgaagc agatctacaa gacccccccc atcaaggact tcggcggctt caacttctcc     2400

cagatcctgc ccgacccctc caagccctcc aagcggtcct tcatcgagga cctgctgttc     2460

aacaaggtga ccctggccga cgccggcttc atcaagcagt acggcgactg cctgggcgac     2520

atcgccgccc gcgacctgat ctgcgcccag aagttcaacg gcctgaccgt gctgcccccc     2580

ctgctgaccg acgagatgat cgcccagtac acctccgccc tgctggccgg caccatcacc     2640

tccggctgga ccttcggcgc cggcgccgcc ctgcagatcc ccttcgccat gcagatggcc     2700

taccgcttca acggcatcgg cgtgacccag aacgtgctgt acgagaacca gaagctgatc     2760

gccaaccagt tcaactccgc catcggcaag atccaggact ccctgtcctc caccgcctcc     2820

gccctgggca agctgcagga cgtggtgaac cagaacgccc aggccctgaa caccctggtg     2880

aagcagctgt cctccaactt cggcgccatc tcctccgtgc tgaacgacat cctgtcccgc     2940

ctggacaagg tggaggccga ggtgcagatc gaccgcctga tcaccggccg cctgcagtcc     3000

ctgcagacct acgtgaccca gcagctgatc cgcgccgccg agatccgcgc ctccgccaac     3060

ctggccgcca ccaagatgtc cgagtgcgtg ctgggccagt ccaagcgcgt ggacttctgc     3120

ggcaagggct accacctgat gtccttcccc cagtccgccc cccacggcgt ggtgttcctg     3180

cacgtgacct acgtgcccgc ccaggagaag aacttcacca ccgcccccgc catctgccac     3240

gacggcaagg cccacttccc ccgcgagggc gtgttcgtgt ccaacggcac ccactggttc     3300

gtgacccagc gcaacttcta cgagccccag atcatcacca ccgacaacac cttcgtgtcc     3360

ggcaactgcg acgtggtgat cggcatcgtg aacaacaccg tgtacgaccc cctgcagccc     3420

gagctggact ccttcaagga ggagctggac aagtacttca agaaccacac ctcccccgac     3480

gtggacctgg gcgacatctc cggcatcaac gcctccgtgg tgaacatcca gaaggagatc     3540

gaccgcctga acgaggtggc caagaacctg aacgagtccc tgatcgacct gcaggagctg     3600

ggcaagtacg agcagtacat caagtggccc tggtacatct ggctgggctt catcgccggc     3660

ctgatcgcca tcgtgatggt gaccatcatg ctgtgctgca tgacctcctg ctgctcctgc     3720

ctgaagggct gctgctcctg cggctcctgc tgcaagttcg acgaggacga ctccgagccc     3780

gtgctgaagg gcgtgaagct gcactacacc taa                                  3813


<210>  5
<211>  4224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Full-length S protein deleted furin 
       cleavage site mRNA-coding DNA sequence

<400>  5
agcataaaag tctcaacaca acatatacaa aacaaacgaa tctcaagcaa tcaagcattc       60

tacttctatt gcagcaattt aaatcatttc ttttaaagca aaagcaattt tctgaaaatt      120

ttcaccattt acgaacgata gcgctatgtt cgtgttcctg gtgctgctgc ccctggtgtc      180

ctcccagtgc gtgaacctga ccacccgcac ccagctgccc cccgcctaca ccaactcctt      240

cacccgcggc gtgtactacc ccgacaaggt gttccgctcc tccgtgctgc actccaccca      300

ggacctgttc ctgcccttct tctccaacgt gacctggttc cacgccatcc acgtgtccgg      360

caccaacggc accaagcggt tcgacaaccc cgtgctgccc ttcaacgacg gcgtgtactt      420

cgcctccacc gagaagtcca acatcatccg cggctggatc ttcggcacca ccctggactc      480

caagacccag tccctgctga tcgtgaacaa cgccaccaac gtggtgatca aggtgtgcga      540

gttccagttc tgcaacgacc ccttcctggg cgtgtactac cacaagaaca acaagtcctg      600

gatggagtcc gagttccgcg tgtactcctc cgccaacaac tgcaccttcg agtacgtgtc      660

ccagcccttc ctgatggacc tggagggcaa gcagggcaac ttcaagaacc tgcgcgagtt      720

cgtgttcaag aacatcgacg gctacttcaa gatctactcc aagcacaccc ccatcaacct      780

ggtgcgcgac ctgccccagg gcttctccgc cctggagccc ctggtggacc tgcccatcgg      840

catcaacatc acccgcttcc agaccctgct ggccctgcac cgctcctacc tgacccccgg      900

cgactcctcc tccggctgga ccgccggcgc cgccgcctac tacgtgggct acctgcagcc      960

ccgcaccttc ctgctgaagt acaacgagaa cggcaccatc accgacgccg tggactgcgc     1020

cctggacccc ctgtccgaga ccaagtgcac cctgaagtcc ttcaccgtgg agaagggcat     1080

ctaccagacc tccaacttcc gcgtgcagcc caccgagtcc atcgtgcgct tccccaacat     1140

caccaacctg tgccccttcg gcgaggtgtt caacgccacc cgcttcgcct ccgtgtacgc     1200

ctggaaccgc aagcgcatct ccaactgcgt ggccgactac tccgtgctgt acaactccgc     1260

ctccttctcc accttcaagt gctacggcgt gtcccccacc aagctgaacg acctgtgctt     1320

caccaacgtg tacgccgact ccttcgtgat ccgcggcgac gaggtgcgcc agatcgcccc     1380

cggccagacc ggcaagatcg ccgactacaa ctacaagctg cccgacgact tcaccggctg     1440

cgtgatcgcc tggaactcca acaacctgga ctccaaggtg ggcggcaact acaactacct     1500

gtaccgcctg ttccgcaagt ccaacctgaa gcccttcgag cgcgacatct ccaccgagat     1560

ctaccaggcc ggctccaccc cctgcaacgg cgtggagggc ttcaactgct acttccccct     1620

gcagtcctac ggcttccagc ccaccaacgg cgtgggctac cagccctacc gcgtggtggt     1680

gctgtccttc gagctgctgc acgcccccgc caccgtgtgc ggccccaaga agtccaccaa     1740

cctggtgaag aacaagtgcg tgaacttcaa cttcaacggc ctgaccggca ccggcgtgct     1800

gaccgagtcc aacaagaagt tcctgccctt ccagcagttc ggccgcgaca tcgccgacac     1860

caccgacgcc gtgcgcgacc cccagaccct ggagatcctg gacatcaccc cctgctcctt     1920

cggcggcgtg tccgtgatca cccccggcac caacacctcc aaccaggtgg ccgtgctgta     1980

ccaggacgtg aactgcaccg aggtgcccgt ggccatccac gccgaccagc tgacccccac     2040

ctggcgcgtg tactccaccg gctccaacgt gttccagacc cgcgccggct gcctgatcgg     2100

cgccgagcac gtgaacaact cctacgagtg cgacatcccc atcggcgccg gcatctgcgc     2160

ctcctaccag acccagacca actcccccgc ctccgtggcc tcccagtcca tcatcgccta     2220

caccatgtcc ctgggcgccg agaactccgt ggcctactcc aacaactcca tcgccatccc     2280

caccaacttc accatctccg tgaccaccga gatcctgccc gtgtccatga ccaagacctc     2340

cgtggactgc accatgtaca tctgcggcga ctccaccgag tgctccaacc tgctgctgca     2400

gtacggctcc ttctgcaccc agctgaaccg cgccctgacc ggcatcgccg tggagcagga     2460

caagaacacc caggaggtgt tcgcccaggt gaagcagatc tacaagaccc cccccatcaa     2520

ggacttcggc ggcttcaact tctcccagat cctgcccgac ccctccaagc cctccaagcg     2580

gtccttcatc gaggacctgc tgttcaacaa ggtgaccctg gccgacgccg gcttcatcaa     2640

gcagtacggc gactgcctgg gcgacatcgc cgcccgcgac ctgatctgcg cccagaagtt     2700

caacggcctg accgtgctgc cccccctgct gaccgacgag atgatcgccc agtacacctc     2760

cgccctgctg gccggcacca tcacctccgg ctggaccttc ggcgccggcg ccgccctgca     2820

gatccccttc gccatgcaga tggcctaccg cttcaacggc atcggcgtga cccagaacgt     2880

gctgtacgag aaccagaagc tgatcgccaa ccagttcaac tccgccatcg gcaagatcca     2940

ggactccctg tcctccaccg cctccgccct gggcaagctg caggacgtgg tgaaccagaa     3000

cgcccaggcc ctgaacaccc tggtgaagca gctgtcctcc aacttcggcg ccatctcctc     3060

cgtgctgaac gacatcctgt cccgcctgga caaggtggag gccgaggtgc agatcgaccg     3120

cctgatcacc ggccgcctgc agtccctgca gacctacgtg acccagcagc tgatccgcgc     3180

cgccgagatc cgcgcctccg ccaacctggc cgccaccaag atgtccgagt gcgtgctggg     3240

ccagtccaag cgcgtggact tctgcggcaa gggctaccac ctgatgtcct tcccccagtc     3300

cgccccccac ggcgtggtgt tcctgcacgt gacctacgtg cccgcccagg agaagaactt     3360

caccaccgcc cccgccatct gccacgacgg caaggcccac ttcccccgcg agggcgtgtt     3420

cgtgtccaac ggcacccact ggttcgtgac ccagcgcaac ttctacgagc cccagatcat     3480

caccaccgac aacaccttcg tgtccggcaa ctgcgacgtg gtgatcggca tcgtgaacaa     3540

caccgtgtac gaccccctgc agcccgagct ggactccttc aaggaggagc tggacaagta     3600

cttcaagaac cacacctccc ccgacgtgga cctgggcgac atctccggca tcaacgcctc     3660

cgtggtgaac atccagaagg agatcgaccg cctgaacgag gtggccaaga acctgaacga     3720

gtccctgatc gacctgcagg agctgggcaa gtacgagcag tacatcaagt ggccctggta     3780

catctggctg ggcttcatcg ccggcctgat cgccatcgtg atggtgacca tcatgctgtg     3840

ctgcatgacc tcctgctgct cctgcctgaa gggctgctgc tcctgcggct cctgctgcaa     3900

gttcgacgag gacgactccg agcccgtgct gaagggcgtg aagctgcact acacctaaac     3960

tagtagtgac tgactaggat ctggttacca ctaaaccagc ctcaagaaca cccgaatgga     4020

gtctctaagc tacataatac caacttacac ttacaaaatg ttgtccccca aaatgtagcc     4080

attcgtatct gctcctaata aaaagaaagt ttcttcacat tctaaaaaaa aaaaaaaaaa     4140

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     4200

aaaaaaaaaa aaaaaaaaaa aaaa                                            4224


<210>  6
<211>  1270
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Full-length S protein deleted furin 
       cleavage site AA sequence

<400>  6

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Ala Ser Val Ala Ser Gln Ser 
        675                 680                 685             


Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr 
    690                 695                 700                 


Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr 
705                 710                 715                 720 


Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr 
                725                 730                 735     


Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln 
            740                 745                 750         


Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala 
        755                 760                 765             


Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln 
    770                 775                 780                 


Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser 
785                 790                 795                 800 


Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu 
                805                 810                 815     


Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys 
            820                 825                 830         


Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys 
        835                 840                 845             


Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp 
    850                 855                 860                 


Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr 
865                 870                 875                 880 


Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala 
                885                 890                 895     


Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val 
            900                 905                 910         


Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile 
        915                 920                 925             


Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys 
    930                 935                 940                 


Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val 
945                 950                 955                 960 


Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp 
                965                 970                 975     


Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg 
            980                 985                 990         


Leu Ile Thr Gly Arg Leu Gln Ser  Leu Gln Thr Tyr Val  Thr Gln Gln 
        995                 1000                 1005             


Leu Ile  Arg Ala Ala Glu Ile  Arg Ala Ser Ala Asn  Leu Ala Ala 
    1010                 1015                 1020             


Thr Lys  Met Ser Glu Cys Val  Leu Gly Gln Ser Lys  Arg Val Asp 
    1025                 1030                 1035             


Phe Cys  Gly Lys Gly Tyr His  Leu Met Ser Phe Pro  Gln Ser Ala 
    1040                 1045                 1050             


Pro His  Gly Val Val Phe Leu  His Val Thr Tyr Val  Pro Ala Gln 
    1055                 1060                 1065             


Glu Lys  Asn Phe Thr Thr Ala  Pro Ala Ile Cys His  Asp Gly Lys 
    1070                 1075                 1080             


Ala His  Phe Pro Arg Glu Gly  Val Phe Val Ser Asn  Gly Thr His 
    1085                 1090                 1095             


Trp Phe  Val Thr Gln Arg Asn  Phe Tyr Glu Pro Gln  Ile Ile Thr 
    1100                 1105                 1110             


Thr Asp  Asn Thr Phe Val Ser  Gly Asn Cys Asp Val  Val Ile Gly 
    1115                 1120                 1125             


Ile Val  Asn Asn Thr Val Tyr  Asp Pro Leu Gln Pro  Glu Leu Asp 
    1130                 1135                 1140             


Ser Phe  Lys Glu Glu Leu Asp  Lys Tyr Phe Lys Asn  His Thr Ser 
    1145                 1150                 1155             


Pro Asp  Val Asp Leu Gly Asp  Ile Ser Gly Ile Asn  Ala Ser Val 
    1160                 1165                 1170             


Val Asn  Ile Gln Lys Glu Ile  Asp Arg Leu Asn Glu  Val Ala Lys 
    1175                 1180                 1185             


Asn Leu  Asn Glu Ser Leu Ile  Asp Leu Gln Glu Leu  Gly Lys Tyr 
    1190                 1195                 1200             


Glu Gln  Tyr Ile Lys Trp Pro  Trp Tyr Ile Trp Leu  Gly Phe Ile 
    1205                 1210                 1215             


Ala Gly  Leu Ile Ala Ile Val  Met Val Thr Ile Met  Leu Cys Cys 
    1220                 1225                 1230             


Met Thr  Ser Cys Cys Ser Cys  Leu Lys Gly Cys Cys  Ser Cys Gly 
    1235                 1240                 1245             


Ser Cys  Cys Lys Phe Asp Glu  Asp Asp Ser Glu Pro  Val Leu Lys 
    1250                 1255                 1260             


Gly Val  Lys Leu His Tyr Thr  
    1265                 1270 


<210>  7
<211>  714
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Soluble RBD of S protein DNA sequence

<400>  7
atgttcgtgt tcctggtgct gctgcccctg gtgtcctccc agcgcgtgca gcccaccgag       60

tccatcgtgc gcttccccaa catcaccaac ctgtgcccct tcggcgaggt gttcaacgcc      120

acccgcttcg cctccgtgta cgcctggaac cgcaagcgca tctccaactg cgtggccgac      180

tactccgtgc tgtacaactc cgcctccttc tccaccttca agtgctacgg cgtgtccccc      240

accaagctga acgacctgtg cttcaccaac gtgtacgccg actccttcgt gatccgcggc      300

gacgaggtgc gccagatcgc ccccggccag accggcaaga tcgccgacta caactacaag      360

ctgcccgacg acttcaccgg ctgcgtgatc gcctggaact ccaacaacct ggactccaag      420

gtgggcggca actacaacta cctgtaccgc ctgttccgca agtccaacct gaagcccttc      480

gagcgcgaca tctccaccga gatctaccag gccggctcca ccccctgcaa cggcgtggag      540

ggcttcaact gctacttccc cctgcagtcc tacggcttcc agcccaccaa cggcgtgggc      600

taccagccct accgcgtggt ggtgctgtcc ttcgagctgc tgcacgcccc cgccaccgtg      660

tgcggcccca agaagtccac caacctggtg aagaacaagt gcgtgaactt ctaa            714


<210>  8
<211>  1125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Soluble RBD of S protein mRNA-coding DNA 
       sequence

<400>  8
agcataaaag tctcaacaca acatatacaa aacaaacgaa tctcaagcaa tcaagcattc       60

tacttctatt gcagcaattt aaatcatttc ttttaaagca aaagcaattt tctgaaaatt      120

ttcaccattt acgaacgata gcgctatgtt cgtgttcctg gtgctgctgc ccctggtgtc      180

ctcccagcgc gtgcagccca ccgagtccat cgtgcgcttc cccaacatca ccaacctgtg      240

ccccttcggc gaggtgttca acgccacccg cttcgcctcc gtgtacgcct ggaaccgcaa      300

gcgcatctcc aactgcgtgg ccgactactc cgtgctgtac aactccgcct ccttctccac      360

cttcaagtgc tacggcgtgt cccccaccaa gctgaacgac ctgtgcttca ccaacgtgta      420

cgccgactcc ttcgtgatcc gcggcgacga ggtgcgccag atcgcccccg gccagaccgg      480

caagatcgcc gactacaact acaagctgcc cgacgacttc accggctgcg tgatcgcctg      540

gaactccaac aacctggact ccaaggtggg cggcaactac aactacctgt accgcctgtt      600

ccgcaagtcc aacctgaagc ccttcgagcg cgacatctcc accgagatct accaggccgg      660

ctccaccccc tgcaacggcg tggagggctt caactgctac ttccccctgc agtcctacgg      720

cttccagccc accaacggcg tgggctacca gccctaccgc gtggtggtgc tgtccttcga      780

gctgctgcac gcccccgcca ccgtgtgcgg ccccaagaag tccaccaacc tggtgaagaa      840

caagtgcgtg aacttctaaa ctagtagtga ctgactagga tctggttacc actaaaccag      900

cctcaagaac acccgaatgg agtctctaag ctacataata ccaacttaca cttacaaaat      960

gttgtccccc aaaatgtagc cattcgtatc tgctcctaat aaaaagaaag tttcttcaca     1020

ttctaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     1080

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa                     1125


<210>  9
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, Soluble RBD of S protein AA sequence

<400>  9

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Arg Val 
1               5                   10                  15      


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
    210                 215                 220                 


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
225                 230                 235         


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 2019-nCoV N1-Forward Primer

<400>  10
gaccccaaaa tcagcgaaat                                                   20


<210>  11
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 2019-nCoV N1-Reverse Primer

<400>  11
tctggttact gccagttgaa tctg                                              24


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 2019-nCoV N1-Probe

<400>  12
accccgcatt acgtttggtg gacc                                              24


