                         SEQUENCE LISTING

<110>  The Nemours Foundation
       Ma, Zhengyu
 
<120>  Tumor-activated alloreactive and xenoreactive T cells and their 
       use in immunotherapy against cancer

<130>  045009-0053-00-WO-000006

<150>  US 63/048,881
<151>  2020-07-07

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  2219
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct (Gal4-CD3-PGK-BFP)

<400>  1
cggagcactg tcctccgaac gtcggagcac tgtcctccga acgtcggagc actgtcctcc       60

gaacgtcgga gcactgtcct ccgaacggag catgtcctcc gaacgtcgga gcactgtcct      120

ccgaacgact agttaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga      180

accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg      240

accgatccag cctctcgaca ttcgttgcat atggccacca tgcagagtgg gactcattgg      300

agagttttgg gcctgtgcct tctctcagtt ggcgtctggg ggcaggatgg aaacgaggaa      360

atgggaggta taacgcaaac accctacaaa gtctcaatat ccggaacgac ggttattttg      420

acatgccccc agtatccggg ctcagagatt ttgtggcagc ataacgataa aaatatcggg      480

ggcgatgagg acgacaagaa tatcggctcc gatgaagatc atctcagtct gaaagaattt      540

tccgaattgg aacagagcgg atattacgta tgttaccctc gagggtctaa acccgaggat      600

gctaactttt acctttattt gcgcgctagg gtctgcgaaa actgcatgga gatggatgta      660

atgtcagtgg ctacaatagt catagtggat atatgcatta ccgggggact gttgcttctc      720

gtctattact ggtctaaaaa ccgaaaagct aaggctaagc ccgttactcg aggagcaggc      780

gctggtgggc ggcagagggg gcaaaataaa gagcgcccac caccagtgcc taatccggat      840

tacgagccca tacgcaaagg tcagcgggat ctctatagtg ggcttaacca gcgaagaatc      900

tgagcggccg ccttgacttg cggccggccg caactcccac ctgcaacatg cgtgactgac      960

tgaggccgcg actctagagt cgacctgcac gaggttaacg aattctaccg ggtaggggag     1020

gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc     1080

tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct     1140

ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc     1200

cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt     1260

ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc     1320

caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg     1380

ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg     1440

cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc     1500

tgcagcccaa gcttaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca     1560

tggagggcac cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct     1620

acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct     1680

tcgacatcct ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg     1740

gcatccccga cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca     1800

catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc     1860

tcatctacaa cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga     1920

agaaaacact cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg     1980

aaggcagaaa cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacatca     2040

agaccacata tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg     2100

tggactacag actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg     2160

aggtggcagt ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaattaa      2219


<210>  2
<211>  2259
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct (NFAT-CD3-PKG-BFP)

<400>  2
ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt       60

ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt      120

ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt      180

ccccgggaca ttttgacacc cccataatat ttttccagaa ttaacagtat aaattgcatc      240

tcttgttcaa gagttcccta tcactctctt taatcactac tcacagtaac ctcaactcct      300

gaattcccat atggccacca tgcagagtgg gactcattgg agagttttgg gcctgtgcct      360

tctctcagtt ggcgtctggg ggcaggatgg aaacgaggaa atgggaggta taacgcaaac      420

accctacaaa gtctcaatat ccggaacgac ggttattttg acatgccccc agtatccggg      480

ctcagagatt ttgtggcagc ataacgataa aaatatcggg ggcgatgagg acgacaagaa      540

tatcggctcc gatgaagatc atctcagtct gaaagaattt tccgaattgg aacagagcgg      600

atattacgta tgttaccctc gagggtctaa acccgaggat gctaactttt acctttattt      660

gcgcgctagg gtctgcgaaa actgcatgga gatggatgta atgtcagtgg ctacaatagt      720

catagtggat atatgcatta ccgggggact gttgcttctc gtctattact ggtctaaaaa      780

ccgaaaagct aaggctaagc ccgttactcg aggagcaggc gctggtgggc ggcagagggg      840

gcaaaataaa gagcgcccac caccagtgcc taatccggat tacgagccca tacgcaaagg      900

tcagcgggat ctctatagtg ggcttaacca gcgaagaatc tgagcggccg ccttgacttg      960

cggccggccg caactcccac ctgcaacatg cgtgactgac tgaggccgcg actctagagt     1020

cgacctgcac gaggttaacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc     1080

tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc     1140

tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt     1200

cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc     1260

gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac     1320

cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt     1380

cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcggggc     1440

gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc     1500

cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg     1560

agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac     1620

catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg     1680

agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc     1740

ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag     1800

cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg     1860

ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc     1920

agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag     1980

gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc     2040

ctgaagctcg tgggcgggag ccatctgatc gcaaacatca agaccacata tagatccaag     2100

aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga     2160

atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac     2220

tgcgacctcc ctagcaaact ggggcacaag cttaattaa                            2259


<210>  3
<211>  2164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct (AP1-CD3-PKG-BFP)

<400>  3
ttgagtcaag attgagtcat cgttgagtca gacttgagtc actattgagt caactttgag       60

tcatgcttga gtcagtattg agtcaccccg ggacattttg acacccccat aatatttttc      120

cagaattaac agtataaatt gcatctcttg ttcaagagtt ccctatcact ctctttaatc      180

actactcaca gtaacctcaa ctcctgaatt cccatatggc caccatgcag agtgggactc      240

attggagagt tttgggcctg tgccttctct cagttggcgt ctgggggcag gatggaaacg      300

aggaaatggg aggtataacg caaacaccct acaaagtctc aatatccgga acgacggtta      360

ttttgacatg cccccagtat ccgggctcag agattttgtg gcagcataac gataaaaata      420

tcgggggcga tgaggacgac aagaatatcg gctccgatga agatcatctc agtctgaaag      480

aattttccga attggaacag agcggatatt acgtatgtta ccctcgaggg tctaaacccg      540

aggatgctaa cttttacctt tatttgcgcg ctagggtctg cgaaaactgc atggagatgg      600

atgtaatgtc agtggctaca atagtcatag tggatatatg cattaccggg ggactgttgc      660

ttctcgtcta ttactggtct aaaaaccgaa aagctaaggc taagcccgtt actcgaggag      720

caggcgctgg tgggcggcag agggggcaaa ataaagagcg cccaccacca gtgcctaatc      780

cggattacga gcccatacgc aaaggtcagc gggatctcta tagtgggctt aaccagcgaa      840

gaatctgagc ggccgccttg acttgcggcc ggccgcaact cccacctgca acatgcgtga      900

ctgactgagg ccgcgactct agagtcgacc tgcacgaggt taacgaattc taccgggtag      960

gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt     1020

ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac     1080

cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt     1140

tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca     1200

ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca     1260

gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt     1320

ccgggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct     1380

gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt     1440

cgacctgcag cccaagctta ccatgagcga gctgattaag gagaacatgc acatgaagct     1500

gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa     1560

gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt     1620

cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac     1680

ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt     1740

caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg     1800

ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat     1860

gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg     1920

cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa     1980

catcaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta     2040

ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca     2100

gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa     2160

ttaa                                                                  2164


<210>  4
<211>  2198
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct (NFkB-CD3-PKG-BFP)

<400>  4
ggggaaattc ccctagaggg gaaattcccc ttcgggggaa attcccctga cggggaaatt       60

cccctctagg ggaaattccc ctactgggga aattcccctt gcggggaaat tcccctgtac      120

cccgggacat tttgacaccc ccataatatt tttccagaat taacagtata aattgcatct      180

cttgttcaag agttccctat cactctcttt aatcactact cacagtaacc tcaactcctg      240

aattcccata tggccaccat gcagagtggg actcattgga gagttttggg cctgtgcctt      300

ctctcagttg gcgtctgggg gcaggatgga aacgaggaaa tgggaggtat aacgcaaaca      360

ccctacaaag tctcaatatc cggaacgacg gttattttga catgccccca gtatccgggc      420

tcagagattt tgtggcagca taacgataaa aatatcgggg gcgatgagga cgacaagaat      480

atcggctccg atgaagatca tctcagtctg aaagaatttt ccgaattgga acagagcgga      540

tattacgtat gttaccctcg agggtctaaa cccgaggatg ctaactttta cctttatttg      600

cgcgctaggg tctgcgaaaa ctgcatggag atggatgtaa tgtcagtggc tacaatagtc      660

atagtggata tatgcattac cgggggactg ttgcttctcg tctattactg gtctaaaaac      720

cgaaaagcta aggctaagcc cgttactcga ggagcaggcg ctggtgggcg gcagaggggg      780

caaaataaag agcgcccacc accagtgcct aatccggatt acgagcccat acgcaaaggt      840

cagcgggatc tctatagtgg gcttaaccag cgaagaatct gagcggccgc cttgacttgc      900

ggccggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga ctctagagtc      960

gacctgcacg aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct     1020

ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct     1080

cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc     1140

gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg     1200

tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc     1260

gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc     1320

gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcggggcg     1380

ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc     1440

gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga     1500

gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc     1560

atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga     1620

gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct     1680

tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc     1740

agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc     1800

tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca     1860

gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg     1920

ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc     1980

tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga     2040

aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa     2100

tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact     2160

gcgacctccc tagcaaactg gggcacaagc ttaattaa                             2198


<210>  5
<211>  2172
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct (NR4A-CD3-PKG-BFP)

<400>  5
aaaggtcaca gaaaaggtca ctcgaaaggt cacgacaaag gtcacctaaa aggtcacact       60

aaaggtcact gcaaaggtca cgtaaaaggt cacccccggg acattttgac acccccataa      120

tatttttcca gaattaacag tataaattgc atctcttgtt caagagttcc ctatcactct      180

ctttaatcac tactcacagt aacctcaact cctgaattcc catatggcca ccatgcagag      240

tgggactcat tggagagttt tgggcctgtg ccttctctca gttggcgtct gggggcagga      300

tggaaacgag gaaatgggag gtataacgca aacaccctac aaagtctcaa tatccggaac      360

gacggttatt ttgacatgcc cccagtatcc gggctcagag attttgtggc agcataacga      420

taaaaatatc gggggcgatg aggacgacaa gaatatcggc tccgatgaag atcatctcag      480

tctgaaagaa ttttccgaat tggaacagag cggatattac gtatgttacc ctcgagggtc      540

taaacccgag gatgctaact tttaccttta tttgcgcgct agggtctgcg aaaactgcat      600

ggagatggat gtaatgtcag tggctacaat agtcatagtg gatatatgca ttaccggggg      660

actgttgctt ctcgtctatt actggtctaa aaaccgaaaa gctaaggcta agcccgttac      720

tcgaggagca ggcgctggtg ggcggcagag ggggcaaaat aaagagcgcc caccaccagt      780

gcctaatccg gattacgagc ccatacgcaa aggtcagcgg gatctctata gtgggcttaa      840

ccagcgaaga atctgagcgg ccgccttgac ttgcggccgg ccgcaactcc cacctgcaac      900

atgcgtgact gactgaggcc gcgactctag agtcgacctg cacgaggtta acgaattcta      960

ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct     1020

gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag     1080

gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt     1140

caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc     1200

acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct     1260

ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag     1320

gggtgggtcc gggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc     1380

ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc     1440

gggcctttcg acctgcagcc caagcttacc atgagcgagc tgattaagga gaacatgcac     1500

atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac atccgagggc     1560

gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct     1620

ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa gaccttcatc     1680

aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg cttcacatgg     1740

gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc     1800

caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac atccaacggc     1860

cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct gtaccccgct     1920

gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg gagccatctg     1980

atcgcaaaca tcaagaccac atatagatcc aagaaacccg ctaagaacct caagatgcct     2040

ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa cgagacctac     2100

gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa actggggcac     2160

aagcttaatt aa                                                         2172


<210>  6
<211>  8
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (AP-1 Transcription factor binding motif)

<400>  6
ttgagtca                                                                 8


<210>  7
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (AP-1 Transcription factor binding site)

<400>  7
ttgagtcaag attgagtcat cgttgagtca gacttgagtc actattgagt caactttgag       60

tcatgcttga gtcagtattg agtca                                             85


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (NFAT Transcription factor binding motif)

<400>  8
ggaggaaaaa ctgtttcata cagaaggcgt                                        30


<210>  9
<211>  180
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (NFAT Transcription factor binding site)

<400>  9
ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt       60

ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt      120

ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt      180


<210>  10
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (NR4A1 Transcription factor binding motif)

<400>  10
aaaggtcac                                                                9


<210>  11
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (NR4A1 Transcription factor binding site)

<400>  11
aaaggtcaca gaaaaggtca ctcgaaaggt cacgacaaag gtcacctaaa aggtcacact       60

aaaggtcact gcaaaggtca cgtaaaaggt cac                                    93


<210>  12
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (NFkB Transcription factor binding motif)

<400>  12
ggggaaattc ccct                                                         14


<210>  13
<211>  119
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (NFkB Transcription factor binding site)

<400>  13
ggggaaattc ccctagaggg gaaattcccc ttcgggggaa attcccctga cggggaaatt       60

cccctctagg ggaaattccc ctactgggga aattcccctt gcggggaaat tcccctgta       119


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (CD3 zeta gRNA)

<400>  14
ctcctgggaa ccgcacgtgg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (beta-2-microglobulin gRNA)

<400>  15
cgtgagtaaa cctgaatctt                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Sequence (CD3 epsilon gRNA)

<400>  16
agggcatgtc aatattactg                                                   20


