               SEQUENCE LISTING

<110> GENETHON
      INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE
      MEDICALE)
      UNIVERSITE D'EVRY-VAL-D'ESSONNE

<120> A NOVEL MUSCLE SPECIFIC PROMOTER

<130> G143-B-57712 PCT

<150> EP20305796.3
<151> 2020-07-10

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 2239
<212> DNA
<213> Artificial Sequence


<220> 
<223> human ACTA1 promoter -2000 to + 239

<400> 1
tcaaggccca aatgtaagct agtcccctta cgttacatgc agctcatttg ctaagtggtt      60

tttttctagt atctccacta ctcgctgaca caggaggaca caggatgtta aaaaggaaat     120

acagttctgt caattattca cttactctcc aaaatacttg gaagaactaa atatggaacc     180

ataggagact ttatcctcac cgcatagtcc ctatactagt caaactcctt attttttaat     240

tgatcatttt taggaaggta gcattttatt cactagaaca tttttgttaa tacttgttta     300

tttttgggat gaactgccat gatgtgggct acagaggagg gtcgcatatg cttccatccc     360

ccttttagag aatccacacc tgtcccagtt gctgggttcc actaccaaaa gtgaattgca     420

actattttag gagcacttaa gcacatccga aaaatgagtg attctgttct ggcccacacc     480

acatcactga tgtaccccct taaagcatgt ccctgagttc atcacagaag actgctcctc     540

ctgtgccctc cacaaggtta gaactgtcct tgtcttaggg aaaaaggaga gagagagaga     600

gagagagaga gagagagaga gagagagaga gagagaggga caggcaccaa ctgggtaacc     660

tctgctgacc cccactctac tttaccataa gtagctccaa atccttctag aaaatctgaa     720

aggcatagcc ccatatatca gtgatataaa tagaacctgc agcaggctct ggtaaatgat     780

gactacaagg tggactggga ggcagcccgg ccttggcagg catcatcctc taaatataaa     840

gatgagtttg ttcagccttt gcagaaggaa aaactgccac ccatcctaga gtgccgcgtc     900

cttgtccccc caccccctcc aatttattgg gaggaaggac cagctaagcc tcatctagga     960

agagcccctc acccatctcc acctccactc caggtctagc cagtcctggg ttgtgaccct    1020

tgtctttcag ccccaggaga gggacacaca tagtgccacc aaagaggctg ggggagggcc    1080

tcagcccacc aaaacctggg gccagtgcgt cctacaggag gggaaccctc accccttcaa    1140

tccctttagg agacccaagg gcgctgcgcg tccctgaggc ggacagctcc gtgtgctcag    1200

gctttgcgcc tgacaggcct atccccggga gcccccgcgc ctcctccccg gcgctccgcc    1260

ctcgcctccc cccgccagtt gtctatcctg cgacagctgc gcgccctccg gccgccggtg    1320

gccctctgtg cggtggggga aggggtcgac gtggctcagc tttttggatt cagggagctc    1380

gggggtggga agagagaaat ggagttccag gggcgtaaag gagagggagt tcgccttcct    1440

tcccttcctg agactcagga gtgactgctt ctccaatcct cccaagccca ccactccaca    1500

cgactccctc ttcccggtag tcgcaagtgg gagtttgggg atctgagcaa agaacccgaa    1560

gaggagttga aatattggaa gtcagcagtc aggcaccttc ccgagcgccc agggcgctca    1620

gagtggacat ggttggggag gcctttggga caggtgcggt tcccggagcg caggcgcaca    1680

catgcaccca ccggcgaacg cggtgaccct cgccccaccc catcccctcc ggcgggcaac    1740

tgggtcgggt caggaggggc aaacccgcta gggagacact ccatatacgg cccggcccgc    1800

gttacctggg accgggccaa cccgctcctt ctttggtcaa cgcaggggac ccgggcgggg    1860

gcccaggccg cgaaccggcc gagggagggg gctctagtgc ccaacaccca aatatggctc    1920

gagaagggca gcgacattcc tgcggggtgg cgcggaggga atgcccgcgg gctatataaa    1980

acctgagcag agggacaagc ggccaccgca gcggacagcg ccaagtgaag cctcgcttcc    2040

cctccgcggc gaccagggcc cgagccgaga gtagcagttg tagctacccg cccaggtagg    2100

gcaggagttg ggaggggaca gggggacagg gcactaccga ggggaacctg aaggactccg    2160

gggcagaacc cagtcggttc acctggtcag ccccaggcct gcgccctgag cgctgtgcct    2220

cgtctccgga gccacacgc                                                 2239


<210> 2
<211> 324
<212> DNA
<213> Artificial Sequence


<220> 
<223> truncated pACTA1

<400> 2
aaaggcatag ccccatatat cagtgatata aatagaacct gcagcaggct ctggtaaatg      60

atgactacaa ggtggactgg gaggcagccc ggccttggca ggcatcgacc gggccaaccc     120

gctccttctt tggtcaacgc aggggacccg ggcgggggcc caggccgcga accggccgag     180

ggagggggct ctagtgccca acacccaaat atggctcgag aagggcagcg acattcctgc     240

ggggtggcgc ggagggaatg cccgcgggct atataaaacc tgagcagagg gacaagcggc     300

caccgcagcg gacagcgcca agtg                                            324


<210> 3
<211> 106
<212> DNA
<213> Artificial Sequence


<220> 
<223> part 1 truncated pACTA1 (distal)

<400> 3
aaaggcatag ccccatatat cagtgatata aatagaacct gcagcaggct ctggtaaatg     60

atgactacaa ggtggactgg gaggcagccc ggccttggca ggcatc                   106


<210> 4
<211> 218
<212> DNA
<213> Artificial Sequence


<220> 
<223> part 2 truncated pACTA1 (proximal)

<400> 4
gaccgggcca acccgctcct tctttggtca acgcagggga cccgggcggg ggcccaggcc     60

gcgaaccggc cgagggaggg ggctctagtg cccaacaccc aaatatggct cgagaagggc    120

agcgacattc ctgcggggtg gcgcggaggg aatgcccgcg ggctatataa aacctgagca    180

gagggacaag cggccaccgc agcggacagc gccaagtg                            218


<210> 5
<211> 1061
<212> DNA
<213> Artificial Sequence


<220> 
<223> human desmin promoter

<400> 5
taccccctgc cccccacagc tcctctcctg tgccttgttt cccagccatg cgttctcctc      60

tataaatacc cgctctggta tttggggttg gcagctgttg ctgccaggga gatggttggg     120

ttgacatgcg gctcctgaca aaacacaaac ccctggtgtg tgtgggcgtg ggtggtgtga     180

gtagggggat gaatcaggga gggggcgggg gacccagggg gcaggagcca cacaaagtct     240

gtgcgggggt gggagcgcac atagcaattg gaaactgaaa gcttatcaga ccctttctgg     300

aaatcagccc actgtttata aacttgaggc cccaccctcg acagtaccgg ggaggaagag     360

ggcctgcact agtccagagg gaaactgagg ctcagggcta gctcgcccat agacatacat     420

ggcaggcagg ctttggccag gatccctccg cctgccaggc gtctccctgc cctcccttcc     480

tgcctagaga cccccaccct caagcctggc tggtctttgc ctgagaccca aacctcttcg     540

acttcaagag aatatttagg aacaaggtgg tttagggcct ttcctgggaa caggccttga     600

ccctttaaga aatgacccaa agtctctcct tgaccaaaaa ggggaccctc aaactaaagg     660

gaagcctctc ttctgctgtc tcccctgacc ccactccccc ccaccccagg acgaggagat     720

aaccagggct gaaagaggcc cgcctggggg ctgcagacat gcttgctgcc tgccctggcg     780

aaggattggc aggcttgccc gtcacaggac ccccgctggc tgactcaggg gcgcaggcct     840

cttgcggggg agctggcctc cccgccccca cggccacggg ccgccctttc ctggcaggac     900

agcgggatct tgcagctgtc aggggagggg aggcgggggc tgatgtcagg agggatacaa     960

atagtgccga cggctggggg ccctgtctcc cctcgccgca tccactctcc ggccggccgc    1020

ctgcccgccg cctcctccgt gcgcccgcca gcctcgcccg c                        1061


<210> 6
<211> 3319
<212> DNA
<213> Artificial Sequence


<220> 
<223> AAV9-truncated pACTA1-GFP-Luc

<400> 6
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacatt aattttttgc      180

ggccgcaaag gcatagcccc atatatcagt gatataaata gaacctgcag caggctctgg      240

taaatgatga ctacaaggtg gactgggagg cagcccggcc ttggcaggca tcgaccgggc      300

caacccgctc cttctttggt caacgcaggg gacccgggcg ggggcccagg ccgcgaaccg      360

gccgagggag ggggctctag tgcccaacac ccaaatatgg ctcgagaagg gcagcgacat      420

tcctgcgggg tggcgcggag ggaatgcccg cgggctatat aaaacctgag cagagggaca      480

agcggccacc gcagcggaca gcgccaagtg gctagcgcta ccggtcgtcg cgatggtgag      540

caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt      600

aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct      660

gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac      720

caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga      780

cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga      840

cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg      900

catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga      960

gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa     1020

ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta     1080

ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag     1140

cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga     1200

gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt ccggccggac     1260

tcagatctcg agctcaagct tcgaattcga agacgccaaa aacataaaga aaggcccggc     1320

gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag     1380

atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac     1440

ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct     1500

gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt     1560

gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg     1620

tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg     1680

gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat     1740

ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct     1800

acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat     1860

tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca     1920

tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat     1980

tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac     2040

actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct     2100

gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt     2160

ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat     2220

tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca     2280

tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac     2340

acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa     2400

ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt     2460

gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat     2520

tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt     2580

cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc     2640

tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct     2700

tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac     2760

gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt     2820

gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc     2880

aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt     2940

ctcgaagatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg     3000

ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg     3060

caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca     3120

tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac     3180

tcatcaatgt atcttaacgc gtgtagataa gtagcatggc gggttaatca ttaactacaa     3240

ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc     3300

cgggcgacca aaggtcgcc                                                  3319


<210> 7
<211> 4376
<212> DNA
<213> Artificial Sequence


<220> 
<223> AAV9-pACTA1-hCalpain3-2xtarget-miR208a

<400> 7
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc       60

cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg      120

gccaactcca tcactagggg ttcctacgcg tatgcataaa ggcatagccc catatatcag      180

tgatataaat agaacctgca gcaggctctg gtaaatgatg actacaaggt ggactgggag      240

gcagcccggc cttggcaggc atcgaccggg ccaacccgct ccttctttgg tcaacgcagg      300

ggacccgggc gggggcccag gccgcgaacc ggccgaggga gggggctcta gtgcccaaca      360

cccaaatatg gctcgagaag ggcagcgaca ttcctgcggg gtggcgcgga gggaatgccc      420

gcgggctata taaaacctga gcagagggac aagcggccac cgcagcggac agcgccaagt      480

ggctagcgta cacatattga ccaaatcagg gtaattttgc atttgtaatt ttaaaaaatg      540

ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc ctaatctctt      600

tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct aaagaataac      660

agtgataatt tctgggttaa ggcaatagca atatttctgc atataaatat ttctgcatat      720

aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc cagctaccat      780

tctgctttta ttttttggtt gggataaggc tggattattc tgagtccaag ctaggccctt      840

ttgctaatct tgttcatacc tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc      900

tctgtgctgg cccatcactt tggcaaagaa ttcgccacca tgccgaccgt cattagcgca      960

tctgtggctc caaggacagc ggctgagccc cggtccccag ggccagttcc tcacccggcc     1020

cagagcaagg ccactgaggc tgggggtgga aacccaagtg gcatctattc agccatcatc     1080

agccgcaatt ttcctattat cggagtgaaa gagaagacat tcgagcaact tcacaagaaa     1140

tgtctagaaa agaaagttct ttatgtggac cctgagttcc caccggatga gacctctctc     1200

ttttatagcc agaagttccc catccagttc gtctggaaga gacctccgga aatttgcgag     1260

aatccccgat ttatcattga tggagccaac agaactgaca tctgtcaagg agagctaggg     1320

gactgctggt ttctcgcagc cattgcctgc ctgaccctga accagcacct tcttttccga     1380

gtcatacccc atgatcaaag tttcatcgaa aactacgcag ggatcttcca cttccagttc     1440

tggcgctatg gagagtgggt ggacgtggtt atagatgact gcctgccaac gtacaacaat     1500

caactggttt tcaccaagtc caaccaccgc aatgagttct ggagtgctct gctggagaag     1560

gcttatgcta agctccatgg ttcctacgaa gctctgaaag gtgggaacac cacagaggcc     1620

atggaggact tcacaggagg ggtggcagag ttttttgaga tcagggatgc tcctagtgac     1680

atgtacaaga tcatgaagaa agccatcgag agaggctccc tcatgggctg ctccattgat     1740

gatggcacga acatgaccta tggaacctct ccttctggtc tgaacatggg ggagttgatt     1800

gcacggatgg taaggaatat ggataactca ctgctccagg actcagacct cgaccccaga     1860

ggctcagatg aaagaccgac ccggacaatc attccggttc agtatgagac aagaatggcc     1920

tgcgggctgg tcagaggtca cgcctactct gtcacggggc tggatgaggt cccgttcaaa     1980

ggtgagaaag tgaagctggt gcggctgcgg aatccgtggg gccaggtgga gtggaacggt     2040

tcttggagtg atagatggaa ggactggagc tttgtggaca aagatgagaa ggcccgtctg     2100

cagcaccagg tcactgagga tggagagttc tggatgtcct atgaggattt catctaccat     2160

ttcacaaagt tggagatctg caacctcacg gccgatgctc tgcagtctga caagcttcag     2220

acctggacag tgtctgtgaa cgagggccgc tgggtacggg gttgctctgc cggaggctgc     2280

cgcaacttcc cagatacttt ctggaccaac cctcagtacc gtctgaagct cctggaggag     2340

gacgatgacc ctgatgactc ggaggtgatt tgcagcttcc tggtggccct gatgcagaag     2400

aaccggcgga aggaccggaa gctaggggcc agtctcttca ccattggctt cgccatctac     2460

gaggttccca aagagatgca cgggaacaag cagcacctgc agaaggactt cttcctgtac     2520

aacgcctcca aggcccgtag caaaacctac atcaacatga gagaggtgag ccagcgcttc     2580

cgcctgcctc ccagcgagta cgtcatcgtg ccctccacct acgagcccca ccaggagggg     2640

gaattcatcc tccgggtctt ctctgaaaag aggaacctct ctgaggaagt tgaaaatacc     2700

atctccgtgg atcggccagt gaaaaagaaa aaaaccaagc ccatcatctt cgtttcggac     2760

agagcaaaca gcaacaagga gctgggtgtg gaccaggagt cagaggaggg caaaggcaaa     2820

acaagccctg ataagcaaaa gcagtcccca cagccacagc ctggcagctc tgatcaggaa     2880

agtgaggaac agcaacaatt ccggaacatt ttcaagcaga tagcaggaga tgacatggag     2940

atctgtgcag atgagctcaa gaaggtcctt aacacagtcg tgaacaaaca caaggacctg     3000

aagacacacg ggttcacact ggagtcctgc cgtagcatga ttgcgctcat ggatacagat     3060

ggctctggaa agctcaacct gcaggagttc caccacctct ggaacaagat taaggcctgg     3120

cagaaaattt tcaaacacta tgacacagac cagtccggca ccatcaacag ctacgagatg     3180

cgaaatgcag tcaacgacgc aggattccac ctcaacaacc agctctatga catcattacc     3240

atgcggtacg cagacaaaca catgaacatc gactttgaca gtttcatctg ctgcttcgtt     3300

aggctggagg gcatgttcag agcttttcat gcatttgaca aggatggaga tggtatcatc     3360

aagctcaacg ttctggagtg gctgcagctc accatgtatg cctgacgcgt acaagctttt     3420

tgctcgtctt atcctaggac aagctttttg ctcgtcttat gcggcattca ccccaccagt     3480

gcaggctgcc tatcagaaag tggtggctgg tgtggctaat gccctggccc acaagtatca     3540

ctaagctcgc tttcttgctg tccaatttct attaaaggtt cctttgttcc ctaagtccaa     3600

ctactaaact gggggatatt atgaagggcc ttgagcatct ggattctgcc taataaaaaa     3660

catttatttt cattgcaatg atgtatttaa attatttctg aatattttac taaaaaggga     3720

atgtgggagg tcagtgcatt taaaacataa agaaatgaag agctagttca aaccttggga     3780

aaatacacta tatcttaaac tccatgaaag aaggtgaggc tgcaaacagc taatgcacat     3840

tggcaacagc cctgatgcct atgccttatt catccctcag aaaaggattc aagtagaggc     3900

ttgatttgga ggttaaagtt ttgctatgct gtattttaca ttacttattg ttttagctgt     3960

cctcatgaat gtcttttcac tacccatttg cttatcctgc atctctcagc cttgactcca     4020

ctcagttctc ttgcttagag ataccacctt tcccctgaag tgttccttcc atgttttacg     4080

gcgagatggt ttctcctcgc ctggccactc agccttagtt gtctctgttg tcttatagag     4140

gtctacttga agaaggaaaa acagggggca tggtttgact gtcctgtgag cccttcttcc     4200

ctgcctcccc cactcacagt gacccggaat caggaacccc tagtgatgga gttggccact     4260

ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg     4320

ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gagagggagt ggccaa         4376


<210> 8
<211> 821
<212> PRT
<213> Artificial Sequence


<220> 
<223> human calpain 3

<400> 8
Met Pro Thr Val Ile Ser Ala Ser Val Ala Pro Arg Thr Ala Ala Glu 
1               5                   10                  15      
Pro Arg Ser Pro Gly Pro Val Pro His Pro Ala Gln Ser Lys Ala Thr 
            20                  25                  30          
Glu Ala Gly Gly Gly Asn Pro Ser Gly Ile Tyr Ser Ala Ile Ile Ser 
        35                  40                  45              
Arg Asn Phe Pro Ile Ile Gly Val Lys Glu Lys Thr Phe Glu Gln Leu 
    50                  55                  60                  
His Lys Lys Cys Leu Glu Lys Lys Val Leu Tyr Val Asp Pro Glu Phe 
65                  70                  75                  80  
Pro Pro Asp Glu Thr Ser Leu Phe Tyr Ser Gln Lys Phe Pro Ile Gln 
                85                  90                  95      
Phe Val Trp Lys Arg Pro Pro Glu Ile Cys Glu Asn Pro Arg Phe Ile 
            100                 105                 110         
Ile Asp Gly Ala Asn Arg Thr Asp Ile Cys Gln Gly Glu Leu Gly Asp 
        115                 120                 125             
Cys Trp Phe Leu Ala Ala Ile Ala Cys Leu Thr Leu Asn Gln His Leu 
    130                 135                 140                 
Leu Phe Arg Val Ile Pro His Asp Gln Ser Phe Ile Glu Asn Tyr Ala 
145                 150                 155                 160 
Gly Ile Phe His Phe Gln Phe Trp Arg Tyr Gly Glu Trp Val Asp Val 
                165                 170                 175     
Val Ile Asp Asp Cys Leu Pro Thr Tyr Asn Asn Gln Leu Val Phe Thr 
            180                 185                 190         
Lys Ser Asn His Arg Asn Glu Phe Trp Ser Ala Leu Leu Glu Lys Ala 
        195                 200                 205             
Tyr Ala Lys Leu His Gly Ser Tyr Glu Ala Leu Lys Gly Gly Asn Thr 
    210                 215                 220                 
Thr Glu Ala Met Glu Asp Phe Thr Gly Gly Val Ala Glu Phe Phe Glu 
225                 230                 235                 240 
Ile Arg Asp Ala Pro Ser Asp Met Tyr Lys Ile Met Lys Lys Ala Ile 
                245                 250                 255     
Glu Arg Gly Ser Leu Met Gly Cys Ser Ile Asp Asp Gly Thr Asn Met 
            260                 265                 270         
Thr Tyr Gly Thr Ser Pro Ser Gly Leu Asn Met Gly Glu Leu Ile Ala 
        275                 280                 285             
Arg Met Val Arg Asn Met Asp Asn Ser Leu Leu Gln Asp Ser Asp Leu 
    290                 295                 300                 
Asp Pro Arg Gly Ser Asp Glu Arg Pro Thr Arg Thr Ile Ile Pro Val 
305                 310                 315                 320 
Gln Tyr Glu Thr Arg Met Ala Cys Gly Leu Val Arg Gly His Ala Tyr 
                325                 330                 335     
Ser Val Thr Gly Leu Asp Glu Val Pro Phe Lys Gly Glu Lys Val Lys 
            340                 345                 350         
Leu Val Arg Leu Arg Asn Pro Trp Gly Gln Val Glu Trp Asn Gly Ser 
        355                 360                 365             
Trp Ser Asp Arg Trp Lys Asp Trp Ser Phe Val Asp Lys Asp Glu Lys 
    370                 375                 380                 
Ala Arg Leu Gln His Gln Val Thr Glu Asp Gly Glu Phe Trp Met Ser 
385                 390                 395                 400 
Tyr Glu Asp Phe Ile Tyr His Phe Thr Lys Leu Glu Ile Cys Asn Leu 
                405                 410                 415     
Thr Ala Asp Ala Leu Gln Ser Asp Lys Leu Gln Thr Trp Thr Val Ser 
            420                 425                 430         
Val Asn Glu Gly Arg Trp Val Arg Gly Cys Ser Ala Gly Gly Cys Arg 
        435                 440                 445             
Asn Phe Pro Asp Thr Phe Trp Thr Asn Pro Gln Tyr Arg Leu Lys Leu 
    450                 455                 460                 
Leu Glu Glu Asp Asp Asp Pro Asp Asp Ser Glu Val Ile Cys Ser Phe 
465                 470                 475                 480 
Leu Val Ala Leu Met Gln Lys Asn Arg Arg Lys Asp Arg Lys Leu Gly 
                485                 490                 495     
Ala Ser Leu Phe Thr Ile Gly Phe Ala Ile Tyr Glu Val Pro Lys Glu 
            500                 505                 510         
Met His Gly Asn Lys Gln His Leu Gln Lys Asp Phe Phe Leu Tyr Asn 
        515                 520                 525             
Ala Ser Lys Ala Arg Ser Lys Thr Tyr Ile Asn Met Arg Glu Val Ser 
    530                 535                 540                 
Gln Arg Phe Arg Leu Pro Pro Ser Glu Tyr Val Ile Val Pro Ser Thr 
545                 550                 555                 560 
Tyr Glu Pro His Gln Glu Gly Glu Phe Ile Leu Arg Val Phe Ser Glu 
                565                 570                 575     
Lys Arg Asn Leu Ser Glu Glu Val Glu Asn Thr Ile Ser Val Asp Arg 
            580                 585                 590         
Pro Val Lys Lys Lys Lys Thr Lys Pro Ile Ile Phe Val Ser Asp Arg 
        595                 600                 605             
Ala Asn Ser Asn Lys Glu Leu Gly Val Asp Gln Glu Ser Glu Glu Gly 
    610                 615                 620                 
Lys Gly Lys Thr Ser Pro Asp Lys Gln Lys Gln Ser Pro Gln Pro Gln 
625                 630                 635                 640 
Pro Gly Ser Ser Asp Gln Glu Ser Glu Glu Gln Gln Gln Phe Arg Asn 
                645                 650                 655     
Ile Phe Lys Gln Ile Ala Gly Asp Asp Met Glu Ile Cys Ala Asp Glu 
            660                 665                 670         
Leu Lys Lys Val Leu Asn Thr Val Val Asn Lys His Lys Asp Leu Lys 
        675                 680                 685             
Thr His Gly Phe Thr Leu Glu Ser Cys Arg Ser Met Ile Ala Leu Met 
    690                 695                 700                 
Asp Thr Asp Gly Ser Gly Lys Leu Asn Leu Gln Glu Phe His His Leu 
705                 710                 715                 720 
Trp Asn Lys Ile Lys Ala Trp Gln Lys Ile Phe Lys His Tyr Asp Thr 
                725                 730                 735     
Asp Gln Ser Gly Thr Ile Asn Ser Tyr Glu Met Arg Asn Ala Val Asn 
            740                 745                 750         
Asp Ala Gly Phe His Leu Asn Asn Gln Leu Tyr Asp Ile Ile Thr Met 
        755                 760                 765             
Arg Tyr Ala Asp Lys His Met Asn Ile Asp Phe Asp Ser Phe Ile Cys 
    770                 775                 780                 
Cys Phe Val Arg Leu Glu Gly Met Phe Arg Ala Phe His Ala Phe Asp 
785                 790                 795                 800 
Lys Asp Gly Asp Gly Ile Ile Lys Leu Asn Val Leu Glu Trp Leu Gln 
                805                 810                 815     
Leu Thr Met Tyr Ala 
            820     

<210> 9
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> FWD hcalpain3

<400> 9
cgcctccaag gcccgt                                                    16


<210> 10
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> REV hcalpain3

<400> 10
ggcggaagcg ctggct                                                    16


<210> 11
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Probe hcalpain3

<400> 11
ctacatcaac atgagagagg t                                              21


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> FWD Rplp0

<400> 12
ctccaagcag atgcagcaga                                                20


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> REV Rplp0

<400> 13
atagccttgc gcatcatggt                                                20


<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> Probe Rplp0

<400> 14
ccgtggtgct gatgggcaag aa                                             22


