                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Improved fiber-washing in corn wet-milling

<130>  15222-WO-PCT[2]

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  551
<212>  PRT
<213>  Artificial

<220>
<223>  Synthetic construct

<400>  1

Met Asp Glu Lys Asn Leu Leu Glu Asp Pro Asp Ser Asn Leu Ser Ala 
1               5                   10                  15      


Gly Ala Ser Ala Arg Ala Leu Ala Ala Thr Pro Met Leu His Val Gly 
            20                  25                  30          


Gly Arg Tyr Leu Lys Asp Pro Cys Asp Asn Asn Val Leu Leu His Gly 
        35                  40                  45              


Val Ala Ile Thr Pro Ser Pro Trp Phe Asn Gly Cys Gln Tyr Gly Ala 
    50                  55                  60                  


Asn Ser Gly Tyr Cys Thr Trp Asp Asn Tyr Asn Val Gln Gly Cys Leu 
65                  70                  75                  80  


Asn Tyr Asn Lys Ala Val Met Asn Lys Leu Thr Ser Ala Ala Asp Gly 
                85                  90                  95      


Trp Tyr Leu Asn Tyr Ile Arg Leu His Ile Asp Pro Tyr Trp Thr Asn 
            100                 105                 110         


Asp Pro Gly Pro Ser Ile Pro Glu Asn Asp Ile Ser Arg Phe Asn Tyr 
        115                 120                 125             


Asn Arg Leu Val Thr Tyr Thr Asp Gln Val Ile Ile Pro Leu Ile Asn 
    130                 135                 140                 


His Ala Arg Ser Arg Gly Met Tyr Val Ile Leu Arg Pro Pro Gly Val 
145                 150                 155                 160 


Cys Pro Asn Arg Ile Ala Val Asn Asp Ala Tyr His Ser Tyr Leu Lys 
                165                 170                 175     


Thr Val Trp Thr Phe Leu Ser Gln His Pro Gly Leu Lys Asn Ala Asp 
            180                 185                 190         


Asn Val Met Phe Glu Leu Ala Asn Glu Pro Val Glu Ile Leu Gly Thr 
        195                 200                 205             


Asn Gly Thr Trp Gly Gln Thr Gly Asn Glu His Phe Ala Ala Leu Lys 
    210                 215                 220                 


Asn Phe Phe Gln Pro Leu Val Asn Ile Ile Arg Asn Asn Gly Ala Asn 
225                 230                 235                 240 


Asn Val Cys Trp Ile Pro Gly Thr Gly Trp Gln Ser His Tyr Gln Gly 
                245                 250                 255     


Tyr Pro Asn Asn Gln Ile Thr Gly Gly Asn Ile Gly Tyr Ala Val His 
            260                 265                 270         


Ile Tyr Pro Ser Tyr Trp Gly Gly Leu Ser Asn Tyr Gln Ala Phe Gln 
        275                 280                 285             


Asn Ala Trp Asn Ile Asn Val Lys Pro Ile Ala Asp Ile Ala Pro Ile 
    290                 295                 300                 


Ala Ile Thr Glu Thr Asp Trp Ala Pro Gln Gly Tyr Gly Thr Phe Gly 
305                 310                 315                 320 


Ile Gly Thr Thr Gly Thr Ala Gly Gly Ser Gly Phe Gly Ala Asn Leu 
                325                 330                 335     


Lys Tyr Ile Val Asp Gln Ser Gly Asn Val Ser Trp Asn Val Leu Ala 
            340                 345                 350         


Pro Asp Asn Leu Leu His Lys Gly Asp Pro Asn Gly Gly Thr Ala Tyr 
        355                 360                 365             


Asn Asn Asp Trp Glu Ala Cys Ala Ala Pro Val Lys Gln Trp Phe Gln 
    370                 375                 380                 


Gln Tyr Ala Lys Ser Asn Tyr Pro Val Gly Asn Cys Asn Thr Thr Ser 
385                 390                 395                 400 


Ser Leu Val Asn Asn Gly Ile Tyr Glu Ile Glu Phe Gln Thr Asp Ala 
                405                 410                 415     


Asn Lys Val Val Asp Leu Lys Ser Gly Glu Asp Ala Asn Gly Ala Val 
            420                 425                 430         


Leu Arg Pro Trp Thr Arg Asn Gly Ala Ala Ala Gln Arg Trp Val Ala 
        435                 440                 445             


Ile Asp Ala Gly Asn Gly Tyr Trp Arg Phe Val Ser Lys Ala Ser Ala 
    450                 455                 460                 


Thr Asn Arg Cys Ile Thr Leu Ala Ser Asn Ser Asn Thr Leu Gly Thr 
465                 470                 475                 480 


Ser Ile Val Leu Trp Gln Asn Tyr Gly Asn Asp Ala Gln Ala Trp Gln 
                485                 490                 495     


Val Val Ala Val Ser Asn Gly Tyr Tyr Lys Ile Leu Ser Lys Val Asp 
            500                 505                 510         


Pro Thr Arg Gly Trp Asp Ile Pro Asn Cys Thr Met Asp Gly Asn Ser 
        515                 520                 525             


Asn Leu His Leu Trp Asp Tyr Tyr Gly Thr Ser Cys Gln Leu Phe Lys 
    530                 535                 540                 


Phe Lys Tyr Ile Gly Met Asn 
545                 550     


<210>  2
<211>  405
<212>  PRT
<213>  Talaromyces leycettanus

<400>  2

Met Val His Leu Ser Ser Leu Ala Leu Ala Leu Ala Ala Gly Ser Gln 
1               5                   10                  15      


Leu Ala Gln Ala Ala Gly Leu Asn Thr Ala Ala Lys Ala Ile Gly Lys 
            20                  25                  30          


Leu Tyr Phe Gly Thr Ala Thr Asp Asn Pro Glu Leu Ser Asp Ser Thr 
        35                  40                  45              


Tyr Met Gln Glu Thr Asp Asn Thr Asp Asp Phe Gly Gln Leu Thr Pro 
    50                  55                  60                  


Ala Asn Ser Met Lys Trp Asp Ala Thr Glu Pro Ser Gln Asn Thr Phe 
65                  70                  75                  80  


Thr Phe Thr Asn Gly Asp Gln Ile Ala Asn Leu Ala Lys Ser Asn Gly 
                85                  90                  95      


Gln Met Leu Arg Cys His Asn Leu Val Trp Tyr Asn Gln Leu Pro Ser 
            100                 105                 110         


Trp Val Thr Ser Gly Ser Trp Thr Asn Ala Thr Leu Leu Ala Ala Met 
        115                 120                 125             


Lys Asn His Ile Thr Asn Val Val Thr His Tyr Lys Gly Gln Cys Tyr 
    130                 135                 140                 


Ala Trp Asp Val Val Asn Glu Ala Leu Asn Asp Asp Gly Thr Tyr Arg 
145                 150                 155                 160 


Ser Asn Val Phe Tyr Gln Tyr Ile Gly Glu Ala Tyr Ile Pro Ile Ala 
                165                 170                 175     


Phe Ala Thr Ala Ala Ala Ala Asp Pro Asn Ala Lys Leu Tyr Tyr Asn 
            180                 185                 190         


Asp Tyr Asn Ile Glu Tyr Pro Gly Ala Lys Ala Thr Ala Ala Gln Asn 
        195                 200                 205             


Ile Val Lys Met Val Lys Ala Tyr Gly Ala Lys Ile Asp Gly Val Gly 
    210                 215                 220                 


Leu Gln Ser His Phe Ile Val Gly Ser Thr Pro Ser Gln Ser Ser Gln 
225                 230                 235                 240 


Gln Ser Asn Met Ala Ala Phe Thr Ala Leu Gly Val Glu Val Ala Ile 
                245                 250                 255     


Thr Glu Leu Asp Ile Arg Met Thr Leu Pro Ser Thr Ser Ala Leu Leu 
            260                 265                 270         


Ala Gln Gln Ser Thr Asp Tyr Gln Ser Thr Val Ser Ala Cys Val Asn 
        275                 280                 285             


Thr Pro Lys Cys Ile Gly Ile Thr Leu Trp Asp Trp Thr Asp Lys Tyr 
    290                 295                 300                 


Ser Trp Val Pro Asn Thr Phe Ser Gly Gln Gly Asp Ala Cys Pro Trp 
305                 310                 315                 320 


Asp Ser Asn Tyr Gln Lys Lys Pro Ala Tyr Tyr Gly Ile Leu Thr Ala 
                325                 330                 335     


Leu Gly Gly Ser Ala Ser Thr Ser Thr Thr Thr Thr Leu Val Thr Ser 
            340                 345                 350         


Thr Arg Thr Ser Thr Thr Thr Ser Thr Ser Ala Thr Ser Thr Ser Thr 
        355                 360                 365             


Gly Val Ala Gln His Trp Gly Gln Cys Gly Gly Ile Gly Trp Thr Gly 
    370                 375                 380                 


Pro Thr Thr Cys Ala Ser Pro Tyr Thr Cys Gln Glu Leu Asn Pro Tyr 
385                 390                 395                 400 


Tyr Tyr Gln Cys Leu 
                405 


<210>  3
<211>  325
<212>  PRT
<213>  Talaromyces pinophilus

<400>  3

Met His Phe Leu Ala Ala Leu Leu Ala Val Leu Pro Leu Val Ser Gly 
1               5                   10                  15      


Ser Pro Val Pro Glu Lys Arg Ser Gly Cys Ala Leu Pro Ser Thr Tyr 
            20                  25                  30          


Lys Trp Thr Ser Thr Gly Pro Leu Ala Ser Pro Lys Ser Gly Leu Val 
        35                  40                  45              


Ala Leu Arg Asp Tyr Ser His Val Ile Tyr Asn Gly Gln His Leu Val 
    50                  55                  60                  


Tyr Gly Ser Thr Ala Asn Thr Ala Gly Ser Tyr Gly Ser Met Asn Phe 
65                  70                  75                  80  


Gly Leu Phe Ser Asp Trp Ser Glu Met Ser Ser Ala Ser Gln Asn Thr 
                85                  90                  95      


Met Ser Thr Gly Ala Val Ala Pro Thr Ile Phe Tyr Phe Ala Pro Lys 
            100                 105                 110         


Ser Val Trp Ile Leu Ala Tyr Gln Trp Gly Pro Tyr Ala Phe Ser Tyr 
        115                 120                 125             


Arg Thr Ser Thr Asp Pro Ser Asn Ala Asn Gly Trp Ser Ser Pro Gln 
    130                 135                 140                 


Pro Leu Phe Thr Gly Thr Ile Ser Gly Ser Ser Thr Gly Val Ile Asp 
145                 150                 155                 160 


Gln Thr Val Ile Gly Asp Ser Glu Asn Met Tyr Leu Phe Phe Ala Gly 
                165                 170                 175     


Asp Asn Gly His Ile Tyr Arg Ala Ser Met Pro Ile Gly Asp Phe Pro 
            180                 185                 190         


Gly Ser Phe Gly Ser Ala Ser Thr Ile Val Leu Ser Asp Ser Thr Asn 
        195                 200                 205             


Asn Leu Phe Glu Ala Val Glu Val Tyr Thr Val Glu Gly Gln Asn Gln 
    210                 215                 220                 


Tyr Leu Met Ile Val Glu Ala Ile Gly Ala Asn Gly Arg Tyr Phe Arg 
225                 230                 235                 240 


Ser Phe Thr Ala Ser Ser Leu Gly Gly Thr Trp Thr Ala Gln Ala Ser 
                245                 250                 255     


Thr Glu Ser Asn Pro Phe Ala Gly Lys Ala Asn Ser Gly Ala Thr Trp 
            260                 265                 270         


Thr Asn Asp Ile Ser Ser Gly Asp Leu Val Arg Thr Asn Pro Asp Gln 
        275                 280                 285             


Thr Gln Thr Ile Asp Ala Cys Asn Leu Gln Phe Leu Tyr Gln Gly Arg 
    290                 295                 300                 


Ser Thr Ser Ser Gly Gly Asp Tyr Asn Leu Leu Pro Tyr Gln Pro Gly 
305                 310                 315                 320 


Leu Leu Thr Leu Ala 
                325 


<210>  4
<211>  319
<212>  PRT
<213>  Aspergillus niger

<400>  4

Met Val Gln Ile Lys Val Ala Ala Leu Ala Met Leu Phe Ala Ser Gln 
1               5                   10                  15      


Val Leu Ser Glu Pro Ile Glu Pro Arg Gln Ala Ser Val Ser Ile Asp 
            20                  25                  30          


Thr Lys Phe Lys Ala His Gly Lys Lys Tyr Leu Gly Asn Ile Gly Asp 
        35                  40                  45              


Gln Tyr Thr Leu Thr Lys Asn Ser Lys Thr Pro Ala Ile Ile Lys Ala 
    50                  55                  60                  


Asp Phe Gly Ala Leu Thr Pro Glu Asn Ser Met Lys Trp Asp Ala Thr 
65                  70                  75                  80  


Glu Pro Ser Arg Gly Gln Phe Ser Phe Ser Gly Ser Asp Tyr Leu Val 
                85                  90                  95      


Asn Phe Ala Gln Ser Asn Asn Lys Leu Ile Arg Gly His Thr Leu Val 
            100                 105                 110         


Trp His Ser Gln Leu Pro Ser Trp Val Gln Ser Ile Thr Asp Lys Asn 
        115                 120                 125             


Thr Leu Ile Glu Val Met Glu Asn His Ile Thr Thr Val Met Gln His 
    130                 135                 140                 


Tyr Lys Gly Lys Ile Tyr Ala Trp Asp Val Val Asn Glu Ile Phe Asn 
145                 150                 155                 160 


Glu Asp Gly Ser Leu Arg Asp Ser Val Phe Tyr Lys Val Ile Gly Glu 
                165                 170                 175     


Asp Tyr Val Arg Ile Ala Phe Glu Thr Ala Arg Ala Ala Asp Pro Asn 
            180                 185                 190         


Ala Lys Leu Tyr Ile Asn Asp Tyr Asn Leu Asp Ser Ala Ser Tyr Pro 
        195                 200                 205             


Lys Leu Thr Gly Met Val Ser His Val Lys Lys Trp Ile Ala Ala Gly 
    210                 215                 220                 


Ile Pro Ile Asp Gly Ile Gly Ser Gln Thr His Leu Ser Ala Ala Leu 
225                 230                 235                 240 


Asn Ala Leu Ala Gly Ala Gly Thr Lys Glu Ile Ala Val Thr Glu Leu 
                245                 250                 255     


Asp Ile Ala Gly Ala Ser Ser Thr Asp Tyr Val Glu Val Val Glu Ala 
            260                 265                 270         


Cys Leu Asn Gln Pro Lys Cys Ile Gly Ile Thr Val Trp Gly Val Ala 
        275                 280                 285             


Asp Pro Asp Ser Trp Arg Ser Ser Ser Thr Pro Leu Leu Phe Asp Ser 
    290                 295                 300                 


Asn Tyr Asn Pro Lys Pro Ala Tyr Thr Ala Ile Ala Asn Ala Leu 
305                 310                 315                 


