                         SEQUENCE LISTING

<110>  New Portal Limited
 
<120>  Genetically engineered live bacteria and methods of constructing 
       the same

<130>  N026.003.PCTCN

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  64
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(64)
<223>  Asd-F primer

<400>  1
tcacttgcga ctttggctgc tttttgtatg gtgaaagatg tgccaaatag gcgtatcacg       60

aggc                                                                    64


<210>  2
<211>  66
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(66)
<223>  Asd-R primer

<400>  2
gcactagcag gggcggcatc gcgccccaga tttaatgaat aaagatagtg aacctcttcg       60

agggac                                                                  66


<210>  3
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  asd-F2

<400>  3
taggtttccg agcggatcca                                                   20


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  Cm-R3 (reverse)

<400>  4
cctcttacgt gccgatcaac g                                                 21


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<223>  hly operon-F

<400>  5
actcagcagg acaaagcacg                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  hly operon-R

<400>  6
gaggccaatg agtttctctg                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  vat-F

<400>  7
gaactagccc gaagggtatg                                                   20


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  vat-R

<400>  8
tggagatcag atgaactgtg ttc                                               23


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  pks-left-F

<400>  9
aatcaaccca gctgcaaatc                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  pks-left-R

<400>  10
cacccccatc attaaaaacg                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  pks-right-F

<400>  11
agccgtatcc tgctcaaaac                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  pks-right-R

<400>  12
tcggtatgtc cggttaaagc                                                   20


<210>  13
<211>  22
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  papG-F

<400>  13
gcgctaataa tcattatgcg gc                                                22


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  papG-R

<400>  14
caatatcatg agcagcgttg c                                                 21


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  sat-F

<400>  15
ggataaggac tttaatccgc tg                                                22


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  sat-R

<400>  16
ttgatcgcgt tatccacgtt g                                                 21


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  fyrA-F

<400>  17
tgacacggct ttatcctctg                                                   20


<210>  18
<211>  19
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(19)
<223>  fyrA-R

<400>  18
gttgttggct gatgccgag                                                    19


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  iutA-F

<400>  19
aagctggaag gcgtgaaagt                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  iutA-R

<400>  20
taacccgggc tgtagtacag                                                   20


<210>  21
<211>  22
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(22)
<223>  yfcV-F

<400>  21
gagtaagttt gccaaaacag cc                                                22


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  yfcV-R

<400>  22
ctggaaatct ttcggtgtgg t                                                 21


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  chuA-F

<400>  23
gacgaaccaa cggtcaggat                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  chuA-R

<400>  24
tgccgccagt accaaagaca                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  yjaA-F

<400>  25
tgaagtgtca ggagacgctg                                                   20


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  yjaA-R

<400>  26
atggagaatg cgttcctcaa c                                                 21


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  TspE4C2-F

<400>  27
gagtaatgtc ggggcattca                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  TspE4C2-R

<400>  28
cgcgccaaca aagtattacg                                                   20


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(23)
<223>  sfa-F

<400>  29
ccctcgtgga gcctttttta tat                                               23


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  sfa-R

<400>  30
cactgttaac ctcttctggt c                                                 21


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  iutA-F

<400>  31
aagctggaag gcgtgaaagt                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence



<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  iutA-R

<400>  32
taacccgggc tgtagtacag                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  oxb-F

<400>  33
ctgttgtgac cgcttgctct                                                   20


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence


<220>
<221>  misc_feature
<222>  (1)..(21)
<223>  exlA-R

<400>  34
gaggtggaag acaggattgt c                                                 21


<210>  35
<211>  5567
<212>  DNA
<213>  Artificial Sequence




<220>
<221>  misc_feature
<222>  (1)..(286)
<223>  OXB18 promoter and ribosome binding site

<220>
<221>  misc_feature
<222>  (287)..(352)
<223>  start codon ATG followed by pelB leader sequence

<220>
<221>  misc_feature
<222>  (353)..(5387)
<223>  exlA gene of Pseudomonas aeruginosa PA7 without start codon

<220>
<221>  misc_feature
<222>  (5388)..(5545)
<223>  rrnB transcription termination region

<400>  35
atctaagctg ttgtgaccgc ttgctctagc cagctatcga gttgtgaacc gatccatcta       60

gcaattggtc tcgatctagc gataggcttc gatctagcta tgtagaaacg ccgtgtgctc      120

gatcgcttta taaggtccac agtagctgct ataattgctt caacagaaca tattgactat      180

ccggtattac ccggcagatc tttgtcgatc ctaccatcca ctcgacacac ccgccagcgg      240

ccgctgccaa gcttccgagc tctcgaattc aaaggaggta cccaccatga aatacctgct      300

gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cccatagaga      360

caatcctgtc ttccacctct ccccccgggg aaaactccgt tgcctgatcg ccggcctgct      420

gctggcgccc cacctgcccc aggctttcgc cggcgggctg gaggcggccg gcgggccggg      480

cggcacgccg caactgctca accagggcgg cgtgcccatc gtcaatatcg tcgcgccgaa      540

cgccgccggc ctgtcgcaca accagttcct cgactacaac gtcgaccgcc agggcctggt      600

cctcaacaat gcgctgcaag ccggcgcctc gcagctcgcc ggacaactgg cggccaaccc      660

gcagctgcgc ggcgacgccg ccagtgccat cctcaacgaa gtgatcagcc gcaacgcctc      720

ggcgatcaac ggcccgcagg aaatcttcgg ccaggccgcc gactacatcc tggccaaccc      780

caacggcatc tcggtgaacg gcggcagctt catcaacacg ccccatgcca gcctgctggt      840

cggccgtccg gaactggccg acggcaagct gcaggcgctg aacaccaacg acgccgtcgg      900

cgccctgcag atccagggcc agggcctgag caaccgggat ggcagcatcg ccctgctggc      960

gccgcgagtc gacagccaag gcaagatcga ggcgtcggcc gagctcgacc tgaccgtcgg     1020

tcgcaaccgc atcgactacc cgagcggcaa ggtcgagcgc gacccgtccg gcgacgtccg     1080

tccgggcgag cggcgcatcg acgccagcct gttcggcgcc atgcaggccg gacgcatcaa     1140

catcctcagc accgccgagg gcgccggcgt gcgcgtcggc ccggtcggga tcgacggcag     1200

ggacggcgtc gacctgcgct ccgccggcga cctgtcgatc agcggccagg cgcttcccga     1260

caacagcctg aacgccttgc gcgcggcgat ccgcagcgac agcggcaacg tcggcctgca     1320

tgcccgcggc gacctgagcc tggccgccgc cgacgtcagc ggcggcaggg tcgacctcaa     1380

gtccgggcgc aacctgaccc tgggcagcgt ggaaagccgc aacctgcggg aaagccgcga     1440

gcgctggagc aacagcacca tcggcatcac ctgggaaacc tacgaccgca cgcgtaccgt     1500

caccgattcg aagcagcacg gcagccggat cgatgcccgc gccgacgcga gcctggccgc     1560

gcggggcgac agcgaactga gggcggcgac ggtcaaggcc ggcgctaccc tgaaagtctc     1620

cagcggcggc gatacccgtc ttctcgccgc caccgagacc cgcaccgaac gcgaccaggg     1680

cgcgcaccgc aagcacctgt ggaaagccaa ctgggacaag ggcagcagcg agcaacgcag     1740

cgtggccagc agcctcgaag gcgcgcgggt cgaactcggc ggcggccggc gcctgaatct     1800

ggaaggcgcc gacgtggcca gccgcggcga cctcgacctg caagccaaga gcgtcgacat     1860

cggcagcgcc agccgcagcc acagcagccg cgacaacagc tactccggcg acctggtcgg     1920

cggcagcttc ttcggcagcc acggcgatgg cgacagcggc aagaccctgc aacagggcag     1980

ccgggtcaag gccgacggcg cactgacggt gaccgccgat gcggtcgagg tgcgcggcag     2040

ccaggtacgc ggcgcgcgca aggccgaggt agtcagcggg aaaggctcgc tgcgcatcga     2100

cggcgtggag gaaaccgccc acagcaacag ctacagcaag gacagcaagt tcttcggcat     2160

cgccaaggac gagagtcgcc agcgcagcaa ggacagcagc aaccgcgcca gcgaagtacg     2220

ctcggacagc aacctgaccc tgcgcagcgc cgccgacatc gccattcgcg gctcccgggt     2280

cgaggctggc ggcgccctcg ccgccgaggc caagggcaac ctggagatag cctcggcgca     2340

ggagcgccac gacggcaacg acagccgcca cacccgcggc ttcgacgcct atgccggcga     2400

gcagacccca ggcagccgcc aataccgcgc cggggtgcgc taccaggacc agcggaccag     2460

cgtccgccgc gaggaaaccc gcaacagcgg ctccagcctg ggcggcgcct ccctggccgt     2520

gaaggccggc ggcgatctca ccgtgaaggg ggccgagctg aaggccagcg cgggcgacgc     2580

cagcctctcc gggaagaacg tcgccctgct cgccgagcag gacagcaaga cccgcagcag     2640

cgaacagacc accaccggcg gcggcttcta ctataccggc ggcctggacc gtgccggcag     2700

cggtatcgaa gtcggccacc agcggatcga cgagaacgac gccgaaagcc atgcgcgcac     2760

cagccaggtg aacgcgacgg gcaatctcag gatcgacgcc gcccagggca gcctgacgac     2820

gcagggcgcg cgcctggagg ccggcgacag cctggcggtc gccgccggca cggtcgacaa     2880

ccaggccgcc cgcgacagcc agagcagcca gcgccacgac agcggctgga gcggcgacat     2940

cggcgccaac ctcgagtacc gcggcatcgc ccggccgatc gagaaggcgg tcgaaggcgt     3000

cgcccagcgc aaggtccacc agcccggcct gctcgacaac ctggagcagc cgaacgtcgg     3060

cgtcgacctc gagatcagcc accgcgacag ccgcggcgag caacaggcga gccaggcgca     3120

ggtcagcagc ttcgcgggcg gccaggtcga actgaaggtc ggcgacgccc tgcgggacga     3180

gggcacccgt taccaggctc gcagcggagg cctcctcatc gacgccgcca ggcatgacgc     3240

cagggcggcg gagaacacct ccggcagcca tgagcagagc ctcgacgcca aggtcggcgg     3300

gcgcctctac accaccaccg gccaggacct gaacctgcgc ctgagcggca ttggcggcag     3360

cagcgagaac agcgccagcc agaccaccgc ggtggtcggc gaatacgccg cgaagcaggg     3420

cgtcgagatc cgcctcggcg gagacggcct ctaccagggc agccgcttcg acggcggcga     3480

agccggggtc aggctcagcg ccggcggcaa cctggccctg gaacaggcca acgaccggca     3540

gagcgccagc agcgccagcc tgcgtggcga cgccgcgttg agcggcggca tggcccccag     3600

cgccaacggc aaagggctga acgccagcgc cggcctgcaa ctcgaccaca aggccggcga     3660

cagccgggac agccaggcgc gggtcgccga catccaggcc aagggcgcgg tggagctgcg     3720

cagcggcggc gatctggtcc tgcaaggcag caatatcggc agcgcggcag cgaagaccgg     3780

cgacatagtc ctggccgccg gcggcaagct cgacctgcag gccgcccgcg atagccaccg     3840

ggccggggga aacaacctcg gcggcggctt cagcctaggc ggcggcagcg ttcgcgacgc     3900

cgaaacgagc agcaagaacg gcagcgtcag cggcaacttc aacatcggcc gggtcgacga     3960

ggaacgtcac gcgctgaacg gcggcaacct gcacagcgcg accaaggcca gcctttccag     4020

cgccgcagac gacgccaccg cggtacgcct gcaaggcact cgcatcgaag ccgcccaggt     4080

cagcctcgaa gccggcaacg gcggcattct ccaggagtcc gccgaatcca gcgagcggcg     4140

cgacaactgg ggcgtgctgc tcggtgccgg ggccaacggc ggcaagacca ccggcgcgcc     4200

gagcgactac cggagcgact atgccgtcca ggcccgcgcc aaggtcgatg tcgatgtcct     4260

gcgcagccag acccagggcg acagcgtcat ccaggccgac cgggtaatcc tggcgagcca     4320

gggcgacacc cgcctggagg gcgcgcgcat cgacgcggca caggtggacg ggcgcatcgg     4380

cggcgacctg cgggtggaga gccgccagga ccgcgcggag ggcgtgaagg tcaacgtcga     4440

cgcgcgcctg ggcgtggaga agaaccagcc cggcctggtg aacaagctgg cgagcaagac     4500

cggaccgttg aaggacaagc tggaaaccaa ggccgagaat gctttcgaca agcaccgcgg     4560

caagttggag aacggcatcg accgtaatgt cgagcggctc ggcaaggccg gggacaacct     4620

cctcgccaaa gccgaaaagg ccaaggagcg cctgggcgag aagctggtcc gcagcggcag     4680

ctacgaggtc aacccggagc cgcgcggcgc cttcgccagc aagctggaca gggccagggg     4740

ctatctggcg gagaaaggcg aagcgctcgg cgaccggctg tccggcctca agcagcgcct     4800

gtcgccgaac aagaccggta gctatgtggt gaacgacaag cagacggccg gcgccaaggt     4860

cggcaatgcc gccgagaacg tgctgttcgg cgacaagagc ggcgaagcct cggtaacccc     4920

gacgctgtac ctggacgtca gccacgtcag ccgcaactac gtcaccgagg cctccggcat     4980

caccggcagg cagggcgtga acctgcaggt gggcgcagcg acccagctga ccggcgcacg     5040

gatcagcgcc agcgacggca aggtcgacct cggcggctcg cgcgtggaaa cccgcgccct     5100

ggccggcaag gactaccgcg ccgatctcgg cctgaacgtc tccaggtcgc cggtggacct     5160

ggccttcggt atcaaggacg agttcagcca ggagcacgac caggcgaccc gcgacgacca     5220

ggccttcaac ctcggcgccc tgcgcgtcgg cggacgcaac cgcgaccagc agttgcaggc     5280

cggcatcgag cagaaggccg actaatgagt ttaaacggtc tccagcttag gtaacagcct     5340

gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag     5400

tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga     5460

tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa     5520

aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcg                   5567


