                         SEQUENCE LISTING

<110>  Oriengene Biotechnology Co., Ltd
 
<120>  Guide RNA for HSV-1 Gene Editing and Method thereof

<130>  O05-2PI-HT

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6gRNA4-F

<400>  1
caccggcctc ggcgcagatc gatct                                             25


<210>  2
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6gRNA4-R

<400>  2
aaacagatcg atctgcgccg aggcc                                             25


<210>  3
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer MluI-gRNA-F

<400>  3
cgacgcgtga gggcctattt cccatgatt                                         29


<210>  4
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer SpeI-sgRNA-R

<400>  4
gactagtcaa taatcaatgt cacgggtacc tctagagcca tttg                        44


<210>  5
<211>  474
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6-ICP6gRNA fragment

<400>  5
cgacgcgtga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg       60

ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt      120

gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg      180

actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt      240

ggaaaggacg aaacaccggc ctcggcgcag atcgatctgt tttagagcta gaaatagcaa      300

gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt      360

gttttagagc tagaaatagc aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca      420

attctgcaga caaatggctc tagaggtacc cgtgacattg attattgact agtc            474


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6-SOE-F1

<400>  6
gcccaggctc tggaccatta cg                                                22


<210>  7
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6-SOE-R1

<400>  7
gttatgtaac gcggaactcc tcgatctgcg ccgaggcgg                              39


<210>  8
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6-SOE-F3

<400>  8
ggcgtaaatt gtaagcgtct cggacgtcag cgagggc                                37


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer OL40-SOE-R3

<400>  9
aaggttgttg gtgcgaaggt aggc                                              24


<210>  10
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer EGFP-SOE-F2

<400>  10
gcagatcgag gagttccgcg ttacataact tacgg                                  35


<210>  11
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer EGFP-SOE-R2

<400>  11
acgtccgaga cgcttacaat ttacgcctta agatacattg                             40


<210>  12
<211>  1534
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  upstream homology arm

<400>  12
gcccaggctc tggaccatta cgactgtctg atccacagca caccgcacac gctggtcgag       60

cgggggctgc aatcggccct gaagtatgag gagttttacc taaagcgctt tggcgggcac      120

tacatggagt ccgtcttcca gatgtacacc cgcatcgccg gctttttggc ctgccgggcc      180

acgcgcggca tgcgccacat cgccctgggg cgagaggggt cgtggtggga aatgttcaag      240

ttctttttcc accgcctcta cgaccaccag atcgtaccat cgacccccgc catgctgaac      300

ctggggaccc gcaactacta cacctccagc tgctacctgg taaaccccca ggccaccaca      360

aacaaggcga ccctgcgggc catcaccagc aacgtcagtg ccatcctcgc ccgcaacggg      420

ggcatcgggc tatgcgtgca ggcgtttaac gactccggcc ccgggaccgc cagcgtcatg      480

cccgccctca aggtccttga ctcgctggtg gcggcgcaca acaaagagag cgcgcgtccg      540

accggcgcgt gcgtgtacct ggagccgtgg cacaccgacg tgcgggccgt gctccggatg      600

aagggggtcc tcgccggcga agaggcccag cgctgcgaca atatcttcag cgccctctgg      660

atgccagacc tgtttttcaa gcgcctgatt cgccacctgg acggcgagaa gaacgtcaca      720

tggaccctgt tcgaccggga caccagcatg tcgctcgccg actttcacgg ggaggagttc      780

gagaagctct accagcacct cgaggtcatg gggttcggcg agcagatacc catccaggag      840

ctggcctatg gcattgtgcg cagtgcggcc acgaccggga gccccttcgt catgttcaaa      900

gacgcggtga accgccacta catctacgac acccaggggg cggccatcgc cggctccaac      960

ctctgcaccg agatcgtcca tccggcctcc aagcgatcca gtggggtctg caatctggga     1020

agcgtgaatc tggcccgatg cgtctccagg cagacgtttg actttgggcg gctccgcgac     1080

gccgtgcagg cgtgcgtgct gatggtgaac atcatgatcg acagcacgct acaacccacg     1140

ccccagtgca cccgcggcaa cgacaacctg cggtccatgg gaatcggcat gcagggcctg     1200

cacacggcct gcctgaagct ggggctggat ctggagtctg ccgaatttca ggacctgaac     1260

aaacacatcg ccgaggtgat gctgctgtcg gcgatgaaga ccagcaacgc gctgtgcgtt     1320

cgcggggccc gtcccttcaa ccactttaag cgcagcatgt atcgcgccgg ccgctttcac     1380

tgggagcgct ttccggacgc ccggccgcgg tacgagggcg agtgggagat gctacgccag     1440

agcatgatga aacacggcct gcgcaacagc cagtttgtcg cgctgatgcc caccgccgcc     1500

tcggcgcaga tcgaggagtt ccgcgttaca taac                                 1534


<210>  13
<211>  1194
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  downstream homology arm

<400>  13
ggcgtaaatt gtaagcgtct cggacgtcag cgagggcttt gcccccctgt tcaccaacct       60

gttcagcaag gtgacccggg acggcgagac gctgcgcccc aacacgctcc tgctaaagga      120

actggaacgc acgtttagcg ggaagcgcct cctggaggtg atggacagtc tcgacgccaa      180

gcagtggtcc gtggcgcagg cgctcccgtg cctggagccc acccaccccc tccggcgatt      240

caagaccgcg tttgactacg accagaagtt gctgatcgac ctgtgtgcgg accgcgcccc      300

ctacgtcgac catagccaat ccatgaccct gtatgtcacg gagaaggcgg acgggaccct      360

cccagcctcc accctggtcc gccttctggt ccacgcatat aagcgcggac taaaaacagg      420

gatgtactac tgcaaggttc gcaaggcgac caacagcggg gtctttggcg gcgacgacaa      480

cattgtctgc acgagctgcg cgctgtgacc gacaaacccc ctccgcgcca ggcccgccgc      540

cactgtcgtc gccgtcccac gcgctccccc gctgccatgg attccgcggc cccagccctc      600

tcccccgctc tgacggccca tacgggccag agcgcgccgg cggacctggc gatccagatt      660

ccaaagtgcc ccgaccccga gaggtacttc tacacctccc agtgtcccga cattaaccac      720

ctgcgctccc tcagcatcct taaccgctgg ctggaaaccg agcttgtttt cgtgggggac      780

gaggaggacg tctccaagct ttccgagggc gagctcagct tttaccgctt cctcttcgct      840

ttcctgtcgg ccgccgacga cctggttacg gaaaacctgg gcggcctctc cggcctgttt      900

gagcagaagg acattctcca ctactacgtg gagcaggaat gcatcgaagt cgtacactcg      960

cgcgtgtaca acatcatcca gctggtgctt tttcacaaca acgaccaggc gcgccgcgag     1020

tacgtggccg gcaccatcaa ccacccggcc atccgcgcca aggtggactg gctggaagcg     1080

cgggtgcggg aatgtgcctc cgttccggaa aagttcatcc tcatgatcct catcgagggc     1140

atcttttttg ccgcctcgtt tgccgccatc gcctaccttc gcaccaacaa cctt           1194


<210>  14
<211>  1628
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  foreign EGFP gene

<400>  14
gcagatcgag gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc       60

caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg      120

gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca      180

tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc      240

ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt      300

attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata      360

gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt      420

ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca      480

aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg      540

tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg      600

acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg      660

ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc      720

agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc      780

tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc      840

gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc      900

atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag      960

acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc     1020

atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc     1080

cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc     1140

cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc     1200

atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg     1260

agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc     1320

gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa     1380

tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc     1440

tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata     1500

atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc     1560

attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtc     1620

tcggacgt                                                              1628


<210>  15
<211>  4300
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Targeting gene

<400>  15
gcccaggctc tggaccatta cgactgtctg atccacagca caccgcacac gctggtcgag       60

cgggggctgc aatcggccct gaagtatgag gagttttacc taaagcgctt tggcgggcac      120

tacatggagt ccgtcttcca gatgtacacc cgcatcgccg gctttttggc ctgccgggcc      180

acgcgcggca tgcgccacat cgccctgggg cgagaggggt cgtggtggga aatgttcaag      240

ttctttttcc accgcctcta cgaccaccag atcgtaccat cgacccccgc catgctgaac      300

ctggggaccc gcaactacta cacctccagc tgctacctgg taaaccccca ggccaccaca      360

aacaaggcga ccctgcgggc catcaccagc aacgtcagtg ccatcctcgc ccgcaacggg      420

ggcatcgggc tatgcgtgca ggcgtttaac gactccggcc ccgggaccgc cagcgtcatg      480

cccgccctca aggtccttga ctcgctggtg gcggcgcaca acaaagagag cgcgcgtccg      540

accggcgcgt gcgtgtacct ggagccgtgg cacaccgacg tgcgggccgt gctccggatg      600

aagggggtcc tcgccggcga agaggcccag cgctgcgaca atatcttcag cgccctctgg      660

atgccagacc tgtttttcaa gcgcctgatt cgccacctgg acggcgagaa gaacgtcaca      720

tggaccctgt tcgaccggga caccagcatg tcgctcgccg actttcacgg ggaggagttc      780

gagaagctct accagcacct cgaggtcatg gggttcggcg agcagatacc catccaggag      840

ctggcctatg gcattgtgcg cagtgcggcc acgaccggga gccccttcgt catgttcaaa      900

gacgcggtga accgccacta catctacgac acccaggggg cggccatcgc cggctccaac      960

ctctgcaccg agatcgtcca tccggcctcc aagcgatcca gtggggtctg caatctggga     1020

agcgtgaatc tggcccgatg cgtctccagg cagacgtttg actttgggcg gctccgcgac     1080

gccgtgcagg cgtgcgtgct gatggtgaac atcatgatcg acagcacgct acaacccacg     1140

ccccagtgca cccgcggcaa cgacaacctg cggtccatgg gaatcggcat gcagggcctg     1200

cacacggcct gcctgaagct ggggctggat ctggagtctg ccgaatttca ggacctgaac     1260

aaacacatcg ccgaggtgat gctgctgtcg gcgatgaaga ccagcaacgc gctgtgcgtt     1320

cgcggggccc gtcccttcaa ccactttaag cgcagcatgt atcgcgccgg ccgctttcac     1380

tgggagcgct ttccggacgc ccggccgcgg tacgagggcg agtgggagat gctacgccag     1440

agcatgatga aacacggcct gcgcaacagc cagtttgtcg cgctgatgcc caccgccgcc     1500

tcggcgcaga tcgaggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga     1560

ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca     1620

atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca     1680

gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg     1740

cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc     1800

tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt     1860

ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt     1920

ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg     1980

acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg     2040

aaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattctgc     2100

agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc aagggcgagg     2160

agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca     2220

agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt     2280

tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct     2340

acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt     2400

ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact     2460

acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga     2520

agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca     2580

acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca     2640

agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca     2700

cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg     2760

ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg     2820

ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagat     2880

cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct     2940

ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc     3000

ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc     3060

actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa     3120

gcgtctcgga cgtcagcgag ggctttgccc ccctgttcac caacctgttc agcaaggtga     3180

cccgggacgg cgagacgctg cgccccaaca cgctcctgct aaaggaactg gaacgcacgt     3240

ttagcgggaa gcgcctcctg gaggtgatgg acagtctcga cgccaagcag tggtccgtgg     3300

cgcaggcgct cccgtgcctg gagcccaccc accccctccg gcgattcaag accgcgtttg     3360

actacgacca gaagttgctg atcgacctgt gtgcggaccg cgccccctac gtcgaccata     3420

gccaatccat gaccctgtat gtcacggaga aggcggacgg gaccctccca gcctccaccc     3480

tggtccgcct tctggtccac gcatataagc gcggactaaa aacagggatg tactactgca     3540

aggttcgcaa ggcgaccaac agcggggtct ttggcggcga cgacaacatt gtctgcacga     3600

gctgcgcgct gtgaccgaca aaccccctcc gcgccaggcc cgccgccact gtcgtcgccg     3660

tcccacgcgc tcccccgctg ccatggattc cgcggcccca gccctctccc ccgctctgac     3720

ggcccatacg ggccagagcg cgccggcgga cctggcgatc cagattccaa agtgccccga     3780

ccccgagagg tacttctaca cctcccagtg tcccgacatt aaccacctgc gctccctcag     3840

catccttaac cgctggctgg aaaccgagct tgttttcgtg ggggacgagg aggacgtctc     3900

caagctttcc gagggcgagc tcagctttta ccgcttcctc ttcgctttcc tgtcggccgc     3960

cgacgacctg gttacggaaa acctgggcgg cctctccggc ctgtttgagc agaaggacat     4020

tctccactac tacgtggagc aggaatgcat cgaagtcgta cactcgcgcg tgtacaacat     4080

catccagctg gtgctttttc acaacaacga ccaggcgcgc cgcgagtacg tggccggcac     4140

catcaaccac ccggccatcc gcgccaaggt ggactggctg gaagcgcggg tgcgggaatg     4200

tgcctccgtt ccggaaaagt tcatcctcat gatcctcatc gagggcatct tttttgccgc     4260

ctcgtttgcc gccatcgcct accttcgcac caacaacctt                           4300


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer U6-seq-F2

<400>  16
tgcatatacg atacaaggct gttagag                                           27


<210>  17
<211>  71
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HSV-1 partial ICP6 gene containing a GATC insertion

<400>  17
gccagtttgt cgcgctgatg cccaccgccg cctcggcgca gatcgatctc ggacgtcagc       60

gagggctttg c                                                            71


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICP6 target sequence 1 for gRNA recognition

<400>  18
atcgatctgc gccgaggcgg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICP6 target sequence 2 for gRNA recognition

<400>  19
gagatcgatc tgcgccgagg                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICP6 target sequence 3 for gRNA recognition

<400>  20
tccgagatcg atctgcgccg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICP6 target sequence 4 for gRNA recognition

<400>  21
gcctcggcgc agatcgatct                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICP6 target sequence 5 for gRNA recognition

<400>  22
atcgatctcg gacgtcagcg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ICP6 target sequence 6 for gRNA recognition

<400>  23
tcgatctcgg acgtcagcga                                                   20


<210>  24
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of gRNA 1

<400>  24
uagcuagacg cggcuccgcc                                                   20


<210>  25
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of gRNA 2

<400>  25
cucuagcuag acgcggcucc                                                   20


<210>  26
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of gRNA 3

<400>  26
aggcucuagc uagacgcggc                                                   20


<210>  27
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of gRNA 4

<400>  27
cggagccgcg ucuagcuaga                                                   20


<210>  28
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of gRNA 5

<400>  28
uagcuagagc cugcagucgc                                                   20


<210>  29
<211>  20
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  guide sequence of gRNA 6

<400>  29
agcuagagcc ugcagucgcu                                                   20


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6-F

<400>  30
tgtcggcgat gaagaccagc a                                                 21


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer ICP6-R

<400>  31
gcggaccagg gtggaggct                                                    19


<210>  32
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer HSV1-GFP-KI-F1

<400>  32
gaccactacc agcagaacac cc                                                22


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer HSV1-GFP-KI-R1

<400>  33
cgaacaaacg acccaccaat                                                   20


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer HSV1-GFP-KI-F2

<400>  34
gcccgacaac cactacctg                                                    19


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer HSV1-GFP-KI-R2

<400>  35
cgtccctgac aagaatcaca at                                                22


<210>  36
<211>  10076
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCas9-ICP6gRNA-NeoR

<400>  36
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgagggcc      240

tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg      300

gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat      360

aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta      420

ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca      480

ccggcctcgg cgcagatcga tctgttttag agctagaaat agcaagttaa aataaggcta      540

gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa      600

atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat      660

ggctctagag gtacccgtga cattgattat tgactagtta ttaatagtaa tcaattacgg      720

ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc      780

cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca      840

tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg      900

cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg      960

acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt     1020

ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca     1080

tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg     1140

tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact     1200

ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag     1260

ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact     1320

atagggagac ccaagctggc tagttaagct tggtaccgcc accatggccc caaagaagaa     1380

gcggaaggtc ggtatccacg gtgtcccagc agccatggac aagaagtact ccattgggct     1440

cgatatcggc acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag     1500

caaaaaattc aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg     1560

cgccctcctg ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg     1620

gcgcagatat acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga     1680

gatggctaag gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga     1740

ggataaaaag cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca     1800

tgaaaagtac ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc     1860

tgacttgcgg ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct     1920

catcgagggg gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt     1980

tcagacttac aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa     2040

agcaatcctg agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct     2100

ccctggggag aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac     2160

ccccaacttt aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga     2220

cacctacgat gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct     2280

ttttttggcg gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa     2340

cacggagatc accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca     2400

ccaagacttg actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga     2460

aattttcttc gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca     2520

ggaggaattt tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct     2580

gctggtaaag cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag     2640

catcccccac cagattcacc tgggcgaact gcacgctata ctcaggcggc aagaggattt     2700

ctaccccttt ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc     2760

ctactatgta ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc     2820

agaagagacc atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca     2880

gtccttcatc gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc     2940

taaacactct ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata     3000

cgtcacagaa gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt     3060

ggacctcctc ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt     3120

caaaaagatt gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc     3180

atccctggga acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa     3240

tgaggagaac gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag     3300

ggagatgatt gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa     3360

acagctcaag aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg     3420

gatccgagac aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc     3480

caaccggaac ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca     3540

gaaagcacaa gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg     3600

tagcccagct atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa     3660

agtaatggga aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac     3720

tacccagaag ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa     3780

agaactgggg tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga     3840

gaagctctac ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga     3900

catcaatcgg ctctccgact acgacgtgga tcatatcgtg ccccagtctt ttctcaaaga     3960

tgattctatt gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa     4020

cgtcccctca gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc     4080

caaactgatc acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc     4140

tgagttggat aaagcaggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa     4200

gcacgtggcc caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact     4260

gattcgagag gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga     4320

ctttcagttt tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct     4380

gaatgcagtg gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt     4440

ttacggagac tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat     4500

aggcaaggcc accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga     4560

gattacactg gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac     4620

aggagaaatc gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat     4680

gccgcaggtg aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag     4740

tatcctcccg aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa     4800

gaaatacggc ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt     4860

ggagaaaggg aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat     4920

ggagcgatca agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga     4980

ggtcaaaaaa gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg     5040

ccggaaacga atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc     5100

ctctaaatac gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc     5160

cgaagataat gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat     5220

catcgagcaa ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa     5280

ggtgctttct gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat     5340

tatccacttg tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac     5400

caccatagac agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca     5460

tcagtcaatt acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacaa     5520

gcgtcctgct gctactaaga aagctggtca agctaagaaa aagaaataat ctagagggcc     5580

cttcgaacaa aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca     5640

tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc     5700

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct     5760

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg     5820

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg     5880

ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta     5940

tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt     6000

gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct     6060

cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg     6120

atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag     6180

tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa     6240

tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga     6300

tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa     6360

atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc     6420

tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga     6480

aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca     6540

accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat     6600

tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc     6660

tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag     6720

ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt     6780

cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta     6840

ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg     6900

tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa     6960

ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct     7020

gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg     7080

caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca     7140

atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat     7200

cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac     7260

gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc     7320

gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa     7380

aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag     7440

gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc     7500

ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt     7560

cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca     7620

acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa     7680

tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct     7740

tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca     7800

caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca     7860

tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat     7920

ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag     7980

ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg     8040

cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa     8100

tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca     8160

ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg     8220

taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc     8280

agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc     8340

cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac     8400

tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc     8460

tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata     8520

gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc     8580

acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca     8640

acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag     8700

cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta     8760

gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg     8820

gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc     8880

agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg     8940

acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga     9000

tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg     9060

agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct     9120

gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg     9180

agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc     9240

cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa     9300

ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc     9360

cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt     9420

cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc     9480

ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt     9540

tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc     9600

catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt     9660

gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata     9720

gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga     9780

tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag     9840

catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa     9900

aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt     9960

attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga    10020

aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc        10076


