                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  Circular MiRNA Sponges

<130>  SP102718WO

<150>  SG10202006446X
<151>  2010-07-03

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-132

<400>  1
gcugguaccg acaucugaca au                                                22


<210>  2
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-212

<400>  2
accggcacug accucugaca au                                                22


<210>  3
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-132 bulged binding site

<400>  3
cgaccauggc ucagacuguu a                                                 21


<210>  4
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-212 bulged binding site

<400>  4
uggccgugac uccgacuguu a                                                 21


<210>  5
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-17-5p

<400>  5
caaagugcuu acagugcagg uag                                               23


<210>  6
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-18a-5p

<400>  6
uaaggugcau cuagugcaga uag                                               23


<210>  7
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-17-5p bulged binding site

<400>  7
cuaccugcac ugaugcacuu ug                                                22


<210>  8
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-18a-5p bulged binding site

<400>  8
cuaucugcac uacugcaccu ua                                                22


<210>  9
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-20b-5p

<400>  9
caaagugcuc auagugcagg uag                                               23


<210>  10
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-106a-5p

<400>  10
aaaagugcuu acagugcagg uag                                               23


<210>  11
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-20b-5p bulged binding site

<400>  11
cuaccugcac uaacgcacuu ug                                                22


<210>  12
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  miR-106a-5p bulged binding site

<400>  12
cuaccugcac ugaugcacuu uu                                                22


<210>  13
<211>  545
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of bulged circmR: miR-132 and miR-212

<400>  13
gttggagcag ttggaagtct tattgtacaa catgcttcga ttaagtctcc cacccaaccg       60

cggggaagat cccgaccatg gctcagactg ttatcagcag ctgtgtggcc gtgactccga      120

ctgttaactc gctattagcg accatggctc agactgttaa ctcctatcgt atggccgtga      180

ctccgactgt tatcatgact accgcgacca tggctcagac tgttactata agaagcatgg      240

ccgtgactcc gactgttaat tcggagatcc cgaccatggc tcagactgtt atcagcagct      300

gtgtggccgt gactccgact gttaactcgc tattagcgac catggctcag actgttaact      360

cctatcgtat ggccgtgact ccgactgtta tcatgactac cgcgaccatg gctcagactg      420

ttactataag aagcatggcc gtgactccga ctgttaattc ggagatctgg atagcggccg      480

cgaggtggag gggaagactt tgaggacacc tgtggagagc tcgaattcca gaacgatgaa      540

atagt                                                                  545


<210>  14
<211>  557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of bulged circmiR 1: miR-17 and miR-18a

<400>  14
gttggagcag ttggaagtct tattgtacaa catgcttcga ttaagtctcc cacccaaccg       60

cggggaagat ccctacctgc actgatgcac tttgtcagca gctgtgctat ctgcactact      120

gcaccttaac tcgctattag ctacctgcac tgatgcactt tgactcctat cgtactatct      180

gcactactgc accttatcat gactaccgct acctgcactg atgcactttg ctataagaag      240

cactatctgc actactgcac cttaattcgg agatccctac ctgcactgat gcactttgtc      300

agcagctgtg ctatctgcac tactgcacct taactcgcta ttagctacct gcactgatgc      360

actttgactc ctatcgtact atctgcacta ctgcacctta tcatgactac cgctacctgc      420

actgatgcac tttgctataa gaagcactat ctgcactact gcaccttaat tcggagatct      480

ggatagcggc cgcgaggtgg aggggaagac tttgaggaca cctgtggaga gctcgaattc      540

cagaacgatg aaatagt                                                     557


<210>  15
<211>  557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of bulged circmiR 2: miR-20b and miR-106a

<400>  15
gttggagcag ttggaagtct tattgtacaa catgcttcga ttaagtctcc cacccaaccg       60

cggggaagat ccctacctgc actaacgcac tttgtcagca gctgtgctac ctgcactgat      120

gcacttttac tcgctattag ctacctgcac taacgcactt tgactcctat cgtactacct      180

gcactgatgc actttttcat gactaccgct acctgcacta acgcactttg ctataagaag      240

cactacctgc actgatgcac ttttattcgg agatccctac ctgcactaac gcactttgtc      300

agcagctgtg ctacctgcac tgatgcactt ttactcgcta ttagctacct gcactaacgc      360

actttgactc ctatcgtact acctgcactg atgcactttt tcatgactac cgctacctgc      420

actaacgcac tttgctataa gaagcactac ctgcactgat gcacttttat tcggagatct      480

ggatagcggc cgcgaggtgg aggggaagac tttgaggaca cctgtggaga gctcgaattc      540

cagaacgatg aaatagt                                                     557


<210>  16
<211>  557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of perfect circmR: miR-132 and miR-212

<400>  16
gttggagcag ttggaagtct tattgtacaa catgcttcga ttaagtctcc cacccaaccg       60

cggggaagat cccgaccatg gctgtagact gttatcagca gctgtgtggc cgtgactgga      120

gactgttaac tcgctattag cgaccatggc tgtagactgt taactcctat cgtatggccg      180

tgactggaga ctgttatcat gactaccgcg accatggctg tagactgtta ctataagaag      240

catggccgtg actggagact gttaattcgg agatcccgac catggctgta gactgttatc      300

agcagctgtg tggccgtgac tggagactgt taactcgcta ttagcgacca tggctgtaga      360

ctgttaactc ctatcgtatg gccgtgactg gagactgtta tcatgactac cgcgaccatg      420

gctgtagact gttactataa gaagcatggc cgtgactgga gactgttaat tcggagatct      480

ggatagcggc cgcgaggtgg aggggaagac tttgaggaca cctgtggaga gctcgaattc      540

cagaacgatg aaatagt                                                     557


<210>  17
<211>  569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of perfect circmR1: miR-17 and miR-18a

<400>  17
gttggagcag ttggaagtct tattgtacaa catgcttcga ttaagtctcc cacccaaccg       60

cggggaagat ccctacctgc actgtaagca ctttgtcagc agctgtgcta tctgcactag      120

atgcacctta actcgctatt agctacctgc actgtaagca ctttgactcc tatcgtacta      180

tctgcactag atgcacctta tcatgactac cgctacctgc actgtaagca ctttgctata      240

agaagcacta tctgcactag atgcacctta attcggagat ccctacctgc actgtaagca      300

ctttgtcagc agctgtgcta tctgcactag atgcacctta actcgctatt agctacctgc      360

actgtaagca ctttgactcc tatcgtacta tctgcactag atgcacctta tcatgactac      420

cgctacctgc actgtaagca ctttgctata agaagcacta tctgcactag atgcacctta      480

attcggagat ctggatagcg gccgcgaggt ggaggggaag actttgagga cacctgtgga      540

gagctcgaat tccagaacga tgaaatagt                                        569


<210>  18
<211>  569
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Full sequence of perfect circmR1: miR-20b and miR-106a

<400>  18
gttggagcag ttggaagtct tattgtacaa catgcttcga ttaagtctcc cacccaaccg       60

cggggaagat ccctacctgc actatgagca ctttgtcagc agctgtgcta cctgcactgt      120

aagcactttt actcgctatt agctacctgc actatgagca ctttgactcc tatcgtacta      180

cctgcactgt aagcactttt tcatgactac cgctacctgc actatgagca ctttgctata      240

agaagcacta cctgcactgt aagcactttt attcggagat ccctacctgc actatgagca      300

ctttgtcagc agctgtgcta cctgcactgt aagcactttt actcgctatt agctacctgc      360

actatgagca ctttgactcc tatcgtacta cctgcactgt aagcactttt tcatgactac      420

cgctacctgc actatgagca ctttgctata agaagcacta cctgcactgt aagcactttt      480

attcggagat ctggatagcg gccgcgaggt ggaggggaag actttgagga cacctgtgga      540

gagctcgaat tccagaacga tgaaatagt                                        569


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' Intron forward primer

<400>  19
tagaatcgcc actcctgcat                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' Intron reverse primer

<400>  20
ttgggtggga gacttaatcg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' Intron forward primer

<400>  21
gaggtggagg ggaagacttt                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' Intron reverse primer

<400>  22
tagaatcgcc actcctgcat                                                   20


<210>  23
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-132 perfect binding site

<400>  23
cgaccatggc tgtagactgt ta                                                22


<210>  24
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-132 bulged binding site

<400>  24
cgaccatggc tcagactgtt a                                                 21


<210>  25
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-212 perfect binding site

<400>  25
tggccgtgac tggagactgt ta                                                22


<210>  26
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-212 bulged binding site

<400>  26
tggccgtgac tccgactgtt a                                                 21


<210>  27
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer 1

<400>  27
tcagcagctg tg                                                           12


<210>  28
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer 2

<400>  28
actcctatcg ta                                                           12


<210>  29
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer 3

<400>  29
ctataagaag ca                                                           12


<210>  30
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer 4

<400>  30
actcgctatt ag                                                           12


<210>  31
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer 5

<400>  31
tcatgactac cg                                                           12


<210>  32
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spacer 6

<400>  32
attcggagat cc                                                           12


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Human forward primer

<400>  33
agccacatcg ctcagacacc                                                   20


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GAPDH Human reverse primer

<400>  34
gcccaatacg accaaatcc                                                    19


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18S Mouse forward primer

<400>  35
ttgacggaag ggcaccacca g                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18S Mouse reverse primer

<400>  36
gcaccaccac ccacggaatc g                                                 21


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nppa Mouse forward primer

<400>  37
tcggagccta cgaagatcca                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nppa Mouse reverse primer

<400>  38
gtggcaatgt gaccaagctg                                                   20


<210>  39
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nppb Mouse forward primer

<400>  39
gctgctggag ctgataagag aa                                                22


<210>  40
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nppb Mouse reverse primer

<400>  40
aggtcttcct acaacaactt cagtg                                             25


<210>  41
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Myh6 Mouse forward primer

<400>  41
ctacaagcgc caggctgag                                                    19


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Myh6 Mouse reverse primer

<400>  42
tggagaggtt attcctcgtc g                                                 21


<210>  43
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Myh7 Mouse forward primer

<400>  43
agcattctcc tgctgtttcc tt                                                22


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Myh7 Mouse reverse primer

<400>  44
tgagccttgg attctcaaac g                                                 21


<210>  45
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-17-5p

<400>  45
caaagugcuu acagugcagg uag                                               23


<210>  46
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-18a-5p

<400>  46
uaaggugcau cuagugcaga uag                                               23


<210>  47
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-20b-5p

<400>  47
caaagugcuc auagugcagg uag                                               23


<210>  48
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-106a-5p

<400>  48
aaaagugcuu acagugcagg uag                                               23


<210>  49
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-17-5p perfect binding site

<400>  49
cuaccugcac uguaagcacu uug                                               23


<210>  50
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-18a-5p perfect binding site

<400>  50
cuaucugcac uagaugcacc uua                                               23


<210>  51
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-20b-5p perfect binding site

<400>  51
cuaccugcac uaugagcacu uug                                               23


<210>  52
<211>  23
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  hsa-miR-106a-5p perfect binding site

<400>  52
cuaccugcac uguaagcacu uuu                                               23


