                         SEQUENCE LISTING

<110>  ELIGO BIOSCIENCE
 
<120>  METHOD OF CONTAINMENT OF NUCLEIC ACID VECTORS INTRODUCED IN A 
       MICROBIOME POPULATION

<130>  B3567PC

<150>  US63/048,034
<151>  2020-07-03

<150>  US63/132,190
<151>  2020-12-30

<150>  US63/137,989
<151>  2021-01-15

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primase ori from the PICI of the Escherichia coli strain CFT073

<400>  1
tttgttgcaa tggctgtcta ccctgtctac ctgagtaaag aaaaatacat ttaattcagt       60

acattaactt gggtagacag ccttttttta ctgtctacct actatctacc ctctctacct      120

gattttacct gaatcagaca gggaggtaga tacggggtag atagtggata aaagcactct      180

accccactga aagccgcgcc attactggca tggtggccag taaggtagat aaggtagaca      240

aggggaggca caactcaaaa ctttttaaac gagggggtaa aa                         282


<210>  2
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  restriction site


<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  n is a, c, g, or t

<400>  2
twcannnnnn tgg                                                          13


<210>  3
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primase ori deltaGAAABCC

<400>  3
tttgttgcaa tggctgtcta ccctgtctac ctgagtaaag aaaaatacat ttaattcagt       60

acattaactt gggtagacag ccttttttta ctgtctacct actatctacc ctctctacct      120

gattttacct gaatcagaca gggaggtaga tacggggtag atagtggata aaagcactct      180

accccactga aagcagcgcc attactggca tggtggccag taaggtagat aaggtagaca      240

aggggaggca caactcaaaa ctttttaaac gagggggtaa aa                         282


<210>  4
<211>  282
<212>  DNA
<213>  Artificial Sequence

<220>
<223>   Primase ori devoid of restriction sites

<400>  4
tttgttgcaa tggctgtcta ccctgtctac ctgagtaaag aaaaatacat ttaattcagt       60

atattaactt gggtagacag ccttttttta ctgtctacct tctgtctacc ctctctacct      120

gattttacct gaatcagaca gggaggtaga cacggggtag acagtggata aaagcactct      180

accccactga aagcagtgcc attactggca tggttgccag taaggttgat aaggtagaca      240

aggggaggga caactcaaaa ctttttaaac gagggggtaa aa                         282


<210>  5
<211>  584
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PICI primase-helicase

<400>  5

Met Lys Leu Ala Pro Asn Val Lys Gln Gln Ser Arg Gly Ile Lys His 
1               5                   10                  15      


Lys Glu Thr Glu Val Ile Ile Phe Ala Gly Ser Asp Ala Trp Ser His 
            20                  25                  30          


Ala Lys Gln Trp Gln Glu His Asp Ala Arg Met Ala Gly Asp Asn Glu 
        35                  40                  45              


Pro Pro Val Trp Leu Gly Glu Gln Gln Leu Ser Glu Leu Asp Lys Leu 
    50                  55                  60                  


Gln Ile Val Pro Glu Gly Arg Lys Ser Val Arg Ile Phe Arg Ala Gly 
65                  70                  75                  80  


Tyr Leu Ala Pro Val Met Ile Lys Ala Ile Gly Gln Lys Leu Ala Ala 
                85                  90                  95      


Ala Gly Val Gln Asp Ala Asn Phe Tyr Pro Asp Gly Met His Gly Gln 
            100                 105                 110         


Lys Val Glu Asn Trp Arg Glu Tyr Leu Ala Arg Glu Arg Gln Asn Leu 
        115                 120                 125             


Ser Asp Gly Leu Val Ile Glu Leu Pro Val Lys Gln Lys Ala Gln Leu 
    130                 135                 140                 


Ser Gln Met Ala Asp Ser Glu Arg Ala Gln Leu Leu Ala Asp Arg Phe 
145                 150                 155                 160 


Asp Gly Val Cys Val His Pro Glu Ser Glu Ile Val His Val Trp Cys 
                165                 170                 175     


Gly Gly Val Trp Cys Pro Val Ser Thr Met Glu Leu Ser Arg Glu Met 
            180                 185                 190         


Val Ala Ile Tyr Ser Glu His Arg Ala Thr Phe Ser Lys Arg Val Ile 
        195                 200                 205             


Asn Asn Ala Val Glu Ala Leu Lys Val Ile Ala Glu Pro Met Gly Glu 
    210                 215                 220                 


Pro Ser Gly Asp Leu Leu Pro Phe Ala Asn Gly Ala Leu Asp Leu Lys 
225                 230                 235                 240 


Thr Gly Glu Phe Ser Pro His Thr Pro Glu Asn Trp Ile Thr Thr His 
                245                 250                 255     


Asn Gly Ile Glu Tyr Thr Pro Pro Ala Pro Gly Glu Asn Ile Arg Asp 
            260                 265                 270         


Asn Ala Pro Asn Phe His Lys Trp Leu Glu His Ala Ala Gly Lys Asp 
        275                 280                 285             


Pro Arg Lys Met Met Arg Ile Cys Ala Ala Leu Tyr Met Ile Met Ala 
    290                 295                 300                 


Asn Arg Tyr Asp Trp Gln Met Phe Ile Glu Ala Thr Gly Asp Gly Gly 
305                 310                 315                 320 


Ser Gly Lys Ser Thr Phe Thr His Ile Ala Ser Leu Leu Ala Gly Lys 
                325                 330                 335     


Gln Asn Thr Val Ser Ala Glu Met Thr Ser Leu Asp Asp Ala Gly Gly 
            340                 345                 350         


Arg Ala Gln Val Val Gly Ser Arg Leu Ile Val Leu Ala Asp Gln Pro 
        355                 360                 365             


Lys Tyr Thr Gly Glu Gly Thr Gly Ile Lys Lys Ile Thr Gly Gly Asp 
    370                 375                 380                 


Pro Val Glu Ile Asn Pro Lys Tyr Glu Lys Arg Phe Thr Ala Val Ile 
385                 390                 395                 400 


Arg Ala Val Val Leu Ala Thr Asn Asn Asn Pro Met Ile Phe Thr Glu 
                405                 410                 415     


Arg Ala Gly Gly Val Ala Arg Arg Arg Val Ile Phe Arg Phe Asp Asn 
            420                 425                 430         


Ile Val Ser Glu Ala Glu Lys Asp Arg Glu Leu Pro Glu Lys Ile Ala 
        435                 440                 445             


Ala Glu Ile Pro Val Ile Ile Arg Arg Leu Leu Ala Asn Phe Ala Asp 
    450                 455                 460                 


Pro Glu Lys Ala Arg Ala Leu Leu Ile Glu Gln Arg Asp Gly Asp Glu 
465                 470                 475                 480 


Ala Leu Ala Ile Lys Gln Gln Thr Asp Pro Val Ile Glu Phe Cys Gln 
                485                 490                 495     


Phe Leu Asn Phe Leu Glu Glu Ala Arg Gly Leu Met Met Gly Gly Gly 
            500                 505                 510         


Gly Asp Ser Val Lys Tyr Thr Thr Arg Asn Ser Leu Tyr Arg Val Tyr 
        515                 520                 525             


Leu Ala Phe Met Ala Tyr Ala Gly Arg Ser Lys Pro Leu Asn Val Asn 
    530                 535                 540                 


Asp Phe Gly Lys Ala Met Lys Pro Ala Ala Lys Val Tyr Gly His Glu 
545                 550                 555                 560 


Tyr Ile Thr Arg Lys Val Lys Gly Val Thr Gln Thr Asn Ala Ile Thr 
                565                 570                 575     


Thr Asp Asp Cys Asp Ala Phe Leu 
            580                 


<210>  6
<211>  1752
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PICI primase-helicase

<400>  6
atgaaactgg caccgaacgt aaaacagcag tcacgcggca taaaacacaa agaaacagaa       60

gtcattattt ttgcgggtag tgatgcctgg tcacacgcaa aacaatggca ggaacatgac      120

gcgcgtatgg ccggagataa tgagcctcct gtgtggcttg gggagcagca gttatccgaa      180

ctggataagc tgcaaattgt gccggaaggc agaaaatccg tgcgcatatt cagggccgga      240

tatcttgcgc cagtaatgat aaaggcgatt ggtcagaagc tggcggcggc aggcgtacag      300

gatgcaaatt tttaccctga tggtatgcac ggtcagaagg tggagaactg gcgcgaatat      360

ctggcccgtg agcgccagaa tctttctgat ggtctggtca ttgagcttcc ggtaaagcaa      420

aaggcgcaac tttcgcagat ggcggacagt gagcgcgcgc agctgcttgc cgatcgcttt      480

gatggcgttt gcgtacatcc tgaaagtgaa atcgttcacg tatggtgcgg cggggtatgg      540

tgtccggtca gcacaatgga gctgagccgc gaaatggtgg cgatctattc agagcacagg      600

gccactttca gcaagcgcgt aatcaataac gccgtggaag cgttaaaagt tattgccgaa      660

ccaatgggcg agccgtccgg cgatttgctg ccgttcgcca atggtgcgct tgacctgaaa      720

acgggggaat tttccccgca cacgccggag aactggatca ccacgcacaa cggcattgag      780

tacacgccac cagcacccgg ggagaacatc cgcgataacg cgccaaactt tcataaatgg      840

cttgagcacg cagccggaaa agacccgcgc aagatgatgc gtatatgtgc cgcgctgtac      900

atgattatgg cgaaccggta cgactggcag atgtttattg aggccaccgg agacggcggg      960

agcggtaaaa gtacattcac acacatagcc agccttctgg cagggaaaca aaacacggta     1020

agcgctgaaa tgacatcgct tgatgatgct ggtgggcgtg cgcaggttgt cgggagtcgt     1080

cttatcgtcc tggcagacca gccgaaatat acaggcgaag gaacgggcat caagaaaatc     1140

acgggcggcg accccgtgga aattaacccg aaatatgaaa agcgttttac ggcggtaatc     1200

agggcggtgg tgctggcaac caataacaat ccgatgatat tcaccgaacg ggccggaggt     1260

gtggcacgtc gtcgggtgat attccggttc gataacatcg taagcgaggc agaaaaagac     1320

agggagctac cggaaaagat cgcggctgaa atccctgtca ttatccgccg cttgctggcg     1380

aactttgccg accctgaaaa ggcacgggct ttactcattg aacagcgtga cggtgatgaa     1440

gcactggcaa taaagcaaca gacggatccg gttattgagt tttgccagtt cctgaatttt     1500

ctggaggaag cacgcggcct gatgatgggc ggcggtggcg attcagtgaa gtacacgacc     1560

agaaacagcc tttaccgcgt ctatctggcg tttatggcgt acgcaggcag gagcaaaccg     1620

ctaaacgtaa atgactttgg caaggctatg aagccagccg cgaaagttta cggacatgaa     1680

tatattacgc ggaaagttaa aggagtaacg cagactaacg caataacaac agacgattgc     1740

gacgcgtttt ta                                                         1752


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeted sequence

<400>  7
gccgatctgg ttaactacaa                                                   20


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  targeted sequence

<400>  8
ccagccgatc tggttaacta caa                                               23


