                         SEQUENCE LISTING

<110>  Technische Universitt Mnchen
 
<120>  MODIFIED TERPENE SYNTHASES AND THEIR USE FOR PRODUCTION OF 
       PSEUDOPTEROSIN INTERMEDIATES AND/OR PSEUDOPTEROSINS

<130>  U30985WO

<150>  EP 20183732.5
<151>  2020-07-02

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  321
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  1

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  2
<211>  307
<212>  PRT
<213>  Streptomyces melanosporofaciens

<400>  2

Met Thr Thr Gly Leu Ser Thr Ala Gly Ala Gln Asp Ile Gly Arg Ser 
1               5                   10                  15      


Ser Val Arg Pro Tyr Leu Glu Glu Cys Thr Arg Arg Phe Gln Glu Met 
            20                  25                  30          


Phe Asp Arg His Val Val Thr Arg Pro Thr Lys Val Glu Leu Thr Asp 
        35                  40                  45              


Ala Glu Leu Arg Glu Val Ile Asp Asp Cys Asn Ala Ala Val Ala Pro 
    50                  55                  60                  


Leu Gly Lys Thr Val Ser Asp Glu Arg Trp Ile Ser Tyr Val Gly Val 
65                  70                  75                  80  


Val Leu Trp Ser Gln Ser Pro Arg His Ile Lys Asp Met Glu Ala Phe 
                85                  90                  95      


Lys Ala Val Cys Val Leu Asn Cys Val Thr Phe Val Trp Asp Asp Met 
            100                 105                 110         


Asp Pro Ala Leu His Asp Phe Gly Leu Phe Leu Pro Gln Leu Arg Lys 
        115                 120                 125             


Ile Cys Glu Lys Tyr Tyr Gly Pro Glu Asp Ala Glu Val Ala Tyr Glu 
    130                 135                 140                 


Ala Ala Arg Ala Phe Val Thr Ser Asp His Met Phe Arg Asp Ser Pro 
145                 150                 155                 160 


Ile Lys Ala Ala Leu Cys Thr Thr Ser Pro Glu Gln Tyr Phe Arg Phe 
                165                 170                 175     


Arg Val Thr Asp Ile Gly Val Asp Phe Trp Met Lys Met Ser Tyr Pro 
            180                 185                 190         


Ile Tyr Arg His Pro Glu Phe Thr Glu His Ala Lys Thr Ser Leu Ala 
        195                 200                 205             


Ala Arg Met Thr Thr Arg Gly Leu Thr Ile Val Asn Asp Phe Tyr Ser 
    210                 215                 220                 


Tyr Asp Arg Glu Val Ser Leu Gly Gln Ile Thr Asn Cys Phe Arg Leu 
225                 230                 235                 240 


Cys Asp Val Ser Asp Glu Thr Ala Phe Lys Glu Phe Phe Gln Ala Arg 
                245                 250                 255     


Leu Asp Asp Met Ile Glu Asp Ile Glu Cys Ile Lys Ala Phe Asp Gln 
            260                 265                 270         


Leu Thr Gln Asp Val Phe Leu Asp Leu Ile Tyr Gly Asn Phe Val Trp 
        275                 280                 285             


Thr Thr Ser Asn Lys Arg Tyr Lys Thr Ala Val Asn Asp Val Asn Ser 
    290                 295                 300                 


Arg Ile Gln 
305         


<210>  3
<211>  342
<212>  PRT
<213>  Amycolatopsis benzoatilytica

<400>  3

Met Pro Asn Val Glu Thr Gly Met Leu Pro Lys Gln Tyr Gln Arg Thr 
1               5                   10                  15      


Ser Thr Pro Asp Ile Asn Pro Ala Trp Gln Glu Ala Glu Asn Ala Phe 
            20                  25                  30          


Val Ser Tyr Ala Arg Lys Thr Gly Met Leu Thr Gly Gln Ala Asp Thr 
        35                  40                  45              


Thr Val Met Leu Ser Phe Gly Phe Gly Arg Phe Thr Gly Trp Ala Tyr 
    50                  55                  60                  


Pro Asn Met Pro Thr Tyr Glu Leu Asn Leu Leu Thr Gln Trp His Tyr 
65                  70                  75                  80  


Trp Leu Thr Val Ala Asp Asp Val Ala Asp Ala Met Asn Ser Pro His 
                85                  90                  95      


Asp Ala His Arg Leu Arg Arg His Ile Leu Asp Ala Thr Ser Thr Arg 
            100                 105                 110         


Leu Pro Pro Glu Gln Gly Gly Pro Val Ala Ala Ser Phe His His Leu 
        115                 120                 125             


Trp Trp Arg Thr Ala Pro Ala Gln Ser Thr Glu Trp Gln Gln Arg Ala 
    130                 135                 140                 


Arg Ala Ser Leu Ala Leu Tyr Leu Ser Ala Trp Ala Ser Gln Ser Asp 
145                 150                 155                 160 


Asn Arg Ser Arg Gly His Ile Val Thr Thr His Glu Tyr Ile Asp Ile 
                165                 170                 175     


Arg Arg Arg Ala Val Gly Leu Asp Ile Asn Thr Asp Val Leu Glu Ala 
            180                 185                 190         


Val His His Ile Thr Leu Pro Ala Pro Leu Phe Ala Thr Ser Ser Phe 
        195                 200                 205             


Arg Glu Leu Arg Asn Cys Phe Val Asp Ala Asn Ala Trp Phe Asn Asp 
    210                 215                 220                 


Tyr Tyr Ser Tyr Glu Arg Glu Ala Thr Ser Gly Glu Asn His Asn Leu 
225                 230                 235                 240 


Ala Ile Val Leu Ala His Asn Gln Arg Met Pro Pro Gly Gln Ala Leu 
                245                 250                 255     


Asp Arg Val Leu Glu Met Ile Asn Thr Arg Leu Thr Thr Phe Leu Gln 
            260                 265                 270         


Ile Glu Arg Glu Leu Pro Asp Leu Val Ala Ala Leu Gly Tyr Pro Asp 
        275                 280                 285             


Glu Val Ala Thr Glu Val Leu Arg Tyr Thr Gln Ala Leu Arg Asp Tyr 
    290                 295                 300                 


Thr Tyr Gly His Val Ala Trp Ser Ser Thr Ser Thr Arg Tyr Asn Asp 
305                 310                 315                 320 


Gln Gln Leu Arg Ala Thr Glu Trp Asn Arg Ala His His Ser Asn Asp 
                325                 330                 335     


His Pro Ala Arg Thr Glu 
            340         


<210>  4
<211>  374
<212>  PRT
<213>  Fusarium sporotrichioides

<400>  4

Met Glu Asn Phe Pro Thr Glu Tyr Phe Leu Asn Thr Thr Val Arg Leu 
1               5                   10                  15      


Leu Glu Tyr Ile Arg Tyr Arg Asp Ser Asn Tyr Thr Arg Glu Glu Arg 
            20                  25                  30          


Ile Glu Asn Leu His Tyr Ala Tyr Asn Lys Ala Ala His His Phe Ala 
        35                  40                  45              


Gln Pro Arg Gln Gln Gln Leu Leu Lys Val Asp Pro Lys Arg Leu Gln 
    50                  55                  60                  


Ala Ser Leu Gln Thr Ile Val Gly Met Val Val Tyr Ser Trp Ala Lys 
65                  70                  75                  80  


Val Ser Lys Glu Cys Met Ala Asp Leu Ser Ile His Tyr Thr Tyr Thr 
                85                  90                  95      


Leu Val Leu Asp Asp Ser Lys Asp Asp Pro Tyr Pro Thr Met Val Asn 
            100                 105                 110         


Tyr Phe Asp Asp Leu Gln Ala Gly Arg Glu Gln Ala His Pro Trp Trp 
        115                 120                 125             


Ala Leu Val Asn Glu His Phe Pro Asn Val Leu Arg His Phe Gly Pro 
    130                 135                 140                 


Phe Cys Ser Leu Asn Leu Ile Arg Ser Thr Leu Asp Phe Phe Glu Gly 
145                 150                 155                 160 


Cys Trp Ile Glu Gln Tyr Asn Phe Gly Gly Phe Pro Gly Ser His Asp 
                165                 170                 175     


Tyr Pro Gln Phe Leu Arg Arg Met Asn Gly Leu Gly His Cys Val Gly 
            180                 185                 190         


Ala Ser Leu Trp Pro Lys Glu Gln Phe Asn Glu Arg Ser Leu Phe Leu 
        195                 200                 205             


Glu Ile Thr Ser Ala Ile Ala Gln Met Glu Asn Trp Met Val Trp Val 
    210                 215                 220                 


Asn Asp Leu Met Ser Phe Tyr Lys Glu Phe Asp Asp Glu Arg Asp Gln 
225                 230                 235                 240 


Ile Ser Leu Val Lys Asn Tyr Val Val Ser Asp Glu Ile Ser Leu His 
                245                 250                 255     


Glu Ala Leu Glu Lys Leu Thr Gln Asp Thr Leu His Ser Ser Lys Gln 
            260                 265                 270         


Met Val Ala Val Phe Ser Asp Lys Asp Pro Gln Val Met Asp Thr Ile 
        275                 280                 285             


Glu Cys Phe Met His Gly Tyr Val Thr Trp His Leu Cys Asp Arg Arg 
    290                 295                 300                 


Tyr Arg Leu Ser Glu Ile Tyr Glu Lys Val Lys Glu Glu Lys Thr Glu 
305                 310                 315                 320 


Asp Ala Gln Lys Phe Cys Lys Phe Tyr Glu Gln Ala Ala Asn Val Gly 
                325                 330                 335     


Ala Val Ser Pro Ser Glu Trp Ala Tyr Pro Pro Val Ala Gln Leu Ala 
            340                 345                 350         


Asn Val Arg Ser Lys Asp Val Lys Glu Val Gln Lys Pro Phe Leu Ser 
        355                 360                 365             


Ser Ile Glu Leu Val Glu 
    370                 


<210>  5
<211>  459
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  5

Met Arg Gly Ala Gly Pro Gly Ser Val Arg Phe Ser Ser Leu Phe Ser 
1               5                   10                  15      


Gly Leu Gln Arg Ser Ser Ala Phe Phe Ser Ala Leu Arg Glu Ser Ser 
            20                  25                  30          


Ala Phe Asp Val Arg His Arg Arg Gln Arg Val Ser His Glu Gln Arg 
        35                  40                  45              


Val Pro Ser Val Ala Ala Arg Tyr Gly Gly Thr Ala Val His Ser Glu 
    50                  55                  60                  


Asp Ser Gly Pro Glu Asp Val Met Val Leu Arg Val Pro Glu Leu Gly 
65                  70                  75                  80  


Arg Arg Leu Arg Pro Pro Arg Leu His Pro Ala Thr Pro Ala Val Val 
                85                  90                  95      


Gly Gln Arg Val Pro Trp Leu Arg Asp Ala Leu Ala Asp Ile Glu Phe 
            100                 105                 110         


Pro Gly Gly Thr Pro Glu Asp Phe Leu Arg Gln Asp Val His His Trp 
        115                 120                 125             


Ser Val Tyr Cys Leu Pro Thr Ala Arg Ala Asp Arg Ile Ala Asp Leu 
    130                 135                 140                 


Ser Asn Ile His Glu Leu Ala Phe Ala Met Asp Asp Met Leu Glu Ser 
145                 150                 155                 160 


Val His Asp Thr Asp Ala Asp Ala Asp Val His Thr Ala Ser Asp Leu 
                165                 170                 175     


Asp Pro Ala Ser Gly Ser Ala Ser Asn Pro Gly Ser Gly Ser Gly Ser 
            180                 185                 190         


Gly Phe Gly Ser Gly Phe Gly Arg Ala Ala Arg Arg Glu Arg Val Glu 
        195                 200                 205             


Pro Leu Ala Arg Ala Leu Glu Arg Ala Leu Ala Gly Leu Pro Pro Thr 
    210                 215                 220                 


Gly Pro Val Pro Ser Tyr Leu Arg Ala Ala Asp Gly Cys Phe Arg Thr 
225                 230                 235                 240 


Leu Arg Glu Thr Gly Pro Ala Pro Trp Tyr Arg Arg Phe Gly Glu Ala 
                245                 250                 255     


Val Leu Ser Trp Phe His Gly Ala Val Lys Glu Ser Ser Met Met Ala 
            260                 265                 270         


Thr Gly Arg Leu Thr Gly Phe Glu Glu Thr Leu Asp Ser Arg Ile Asp 
        275                 280                 285             


Thr Ala Gly Gly Phe Phe Ile Ala Thr Ser Ile Glu Tyr Gly Leu Gly 
    290                 295                 300                 


Ile Asp Leu Thr Asp Ala Ile Ala Ala Gly Pro Glu Leu Gly Glu Val 
305                 310                 315                 320 


Glu Arg Ala Ala Trp Val His Gly Val Leu Val Asn Asp Leu Phe Ser 
                325                 330                 335     


Tyr Arg Lys Glu His Phe Gly Glu Ala Gly Arg Ala Pro Asp Gly Arg 
            340                 345                 350         


Ala Pro Asp Gly Arg Ala Asn Thr Leu Arg Val Leu Ala Asp Glu Tyr 
        355                 360                 365             


Ala Cys Ser Leu Gln Glu Ala Val Asp Leu Leu Val Glu His Val Asp 
    370                 375                 380                 


Ala Ala Glu Ala Arg Phe Leu Glu Leu Arg Ala Asp Val Leu Gly Ala 
385                 390                 395                 400 


Pro Leu Gly Ala Arg Pro Gly Val Arg Glu Tyr Leu Asp Ala Leu Glu 
                405                 410                 415     


Leu Val Leu Pro Gly Asn Ile Val Trp Ser Arg Thr Ser Arg Arg Tyr 
            420                 425                 430         


His Gly Thr Gly Cys Pro Trp Thr Gly Thr Ala Arg Thr Ala Met Ala 
        435                 440                 445             


Leu His Pro Asp Arg Thr Val Phe Thr Pro Trp 
    450                 455                 


<210>  6
<211>  974
<212>  DNA
<213>  Streptomyces clavuligerus

<400>  6
atgcatatga ccattagcgt tccgcagctg gattgtccgc tgagccgtcc ggttcatccg       60

gaaggtgaac gtgcagatgc ctatgcagtt gaatggctgc gtggtgttgg tctgatggca      120

gatgaagcag atgcagcacc ggttctggca gttggtctgg gtcgtctggc agcatgttat      180

gttgatgaaa atgcaagctg ggataccctg gcatttatga ccattctgat ggcctggtat      240

gcagaatatg atgatcgtgc aattgatagt accggtgcca ttgatggtct gaccgatgca      300

gaagttgcag aactgcatcg tgcactgggt gaaattctgc gtgatcgtcc ggcacctgat      360

ccgagcgatc cggttcagcg tggtctggcg gatgtttggc gtaccctgaa tggtctggca      420

agcgattggg atcgtgcagc atttgttgat accaccctgc gttattttga agccaatcgt      480

tatgaacgtg tgaatattcg tcgtggtatt ccgcctaccc cgagcgcaca tattggtatg      540

cgtcgtcatg gtggtcatgt ttatggtatg tatattctgg gtgcagccgt taatggttat      600

cgtccggaac gtcgtgttct ggatcatgca gcagttcgtg aactggaaac cctggcagca      660

aattatacca gctgggcaaa tgatctgcat agctttgcac gtgaacatcg tatgggtcag      720

gttaataatc tggtttggag cgttcatcat catgaaggcc tgacctttca gcaggcagca      780

gatcgtgttg cagatctgtg tgataaagaa ctggcagcct atctggaact gcgtcagacc      840

ctgccggaac tgggcattcc gctgaccggt gcaacaggtc gtcatgttcg ttttctggaa      900

gatatgatgt ggtcaatggt tgattggagc gcacgtagtg cacgttatga tgttgttccg      960

gaagcagcat aagc                                                        974


<210>  7
<211>  321
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  7

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Leu Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  8
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  8

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Tyr Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  9
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  9

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Phe Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  10
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  10

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Leu Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  11
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  11

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Ala Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  12
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  12

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Phe Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  13
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  13

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly Ala Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  14
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  14

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly Phe Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  15
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  15

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Ile Met Trp Ser Met 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  16
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  16

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Ile 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  17
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  17

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Thr 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  18
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant of HpS

<400>  18

Met Thr Ile Ser Val Pro Gln Leu Asp Cys Pro Leu Ser Arg Pro Val 
1               5                   10                  15      


His Pro Glu Gly Glu Arg Ala Asp Ala Tyr Ala Val Glu Trp Leu Arg 
            20                  25                  30          


Gly Val Gly Leu Met Ala Asp Glu Ala Asp Ala Ala Pro Val Leu Ala 
        35                  40                  45              


Val Gly Leu Gly Arg Leu Ala Ala Cys Tyr Val Asp Glu Asn Ala Ser 
    50                  55                  60                  


Trp Asp Thr Leu Ala Phe Met Thr Ile Leu Met Ala Trp Tyr Ala Glu 
65                  70                  75                  80  


Tyr Asp Asp Arg Ala Ile Asp Ser Thr Gly Ala Ile Asp Gly Leu Thr 
                85                  90                  95      


Asp Ala Glu Val Ala Glu Leu His Arg Ala Leu Gly Glu Ile Leu Arg 
            100                 105                 110         


Asp Arg Pro Ala Pro Asp Pro Ser Asp Pro Val Gln Arg Gly Leu Ala 
        115                 120                 125             


Asp Val Trp Arg Thr Leu Asn Gly Leu Ala Ser Asp Trp Asp Arg Ala 
    130                 135                 140                 


Ala Phe Val Asp Thr Thr Leu Arg Tyr Phe Glu Ala Asn Arg Tyr Glu 
145                 150                 155                 160 


Arg Val Asn Ile Arg Arg Gly Ile Pro Pro Thr Pro Ser Ala His Ile 
                165                 170                 175     


Gly Met Arg Arg His Gly Gly His Val Tyr Gly Met Tyr Ile Leu Gly 
            180                 185                 190         


Ala Ala Val Asn Gly Tyr Arg Pro Glu Arg Arg Val Leu Asp His Ala 
        195                 200                 205             


Ala Val Arg Glu Leu Glu Thr Leu Ala Ala Asn Tyr Thr Ser Trp Ala 
    210                 215                 220                 


Asn Asp Leu His Ser Phe Ala Arg Glu His Arg Met Gly Gln Val Asn 
225                 230                 235                 240 


Asn Leu Val Trp Ser Val His His His Glu Gly Leu Thr Phe Gln Gln 
                245                 250                 255     


Ala Ala Asp Arg Val Ala Asp Leu Cys Asp Lys Glu Leu Ala Ala Tyr 
            260                 265                 270         


Leu Glu Leu Arg Gln Thr Leu Pro Glu Leu Gly Ile Pro Leu Thr Gly 
        275                 280                 285             


Ala Thr Gly Arg His Val Arg Phe Leu Glu Asp Met Met Trp Ser Cys 
    290                 295                 300                 


Val Asp Trp Ser Ala Arg Ser Ala Arg Tyr Asp Val Val Pro Glu Ala 
305                 310                 315                 320 


Ala 
    


<210>  19
<211>  5
<212>  PRT
<213>  Streptomyces clavuligerus


<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  19

Asp Asp Xaa Xaa Asp 
1               5   


<210>  20
<211>  4
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  20

Asp Asp Met Asp 
1               


<210>  21
<211>  6
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  21

Asp Asp Arg Ala Ile Asp 
1               5       


<210>  22
<211>  9
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  22

Asn Asp Phe Tyr Ser Tyr Asp Arg Glu 
1               5                   


<210>  23
<211>  9
<212>  PRT
<213>  Streptomyces clavuligerus


<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  23

Asn Asp Leu Xaa Ser Phe Ala Arg Glu 
1               5                   


<210>  24
<211>  8
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  24

Trp Thr Thr Ser Asn Lys Arg Tyr 
1               5               


<210>  25
<211>  8
<212>  PRT
<213>  Streptomyces clavuligerus

<400>  25

Trp Ser Ala Arg Ser Ala Arg Tyr 
1               5               


