               SEQUENCE LISTING

<110> Julius-Maximilians-Universität Würzburg

<120> SLAMF7 binding chimeric antigen receptor (CAR) polypeptides

<130> 223733

<150> EP20182866.2
<151> 2020-06-29

<160> 26

<170> BiSSAP 1.3.6

<210> 1
<211> 241
<212> PRT
<213> Artificial Sequence


<220> 
<223> SLAMF7-binding element; huLuc63 VH, linker, huLuc63 VL

<400> 1
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu 
    50                  55                  60                  
Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Pro Asp Gly Asn Tyr Trp Tyr Phe Asp Val Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
145                 150                 155                 160 
Gln Asp Val Gly Ile Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Val Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val 
            180                 185                 190         
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 
Tyr Ser Ser Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 
Lys 
    

<210> 2
<211> 228
<212> PRT
<213> Artificial Sequence


<220> 
<223> IgG4-FC spacer domain; hinge, CH2, CH3 with 4/2 NQ modification

<400> 2
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro Val 
1               5                   10                  15      
Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
            20                  25                  30          
Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
        35                  40                  45              
Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
    50                  55                  60                  
Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser Thr 
65                  70                  75                  80  
Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn 
                85                  90                  95      
Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser 
            100                 105                 110         
Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln 
        115                 120                 125             
Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val 
    130                 135                 140                 
Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
145                 150                 155                 160 
Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
                165                 170                 175     
Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr 
            180                 185                 190         
Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val 
        195                 200                 205             
Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
    210                 215                 220                 
Ser Leu Gly Lys 
225             

<210> 3
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD28 transmembrane domain

<400> 3
Met Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser 
1               5                   10                  15      
Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25              

<210> 4
<211> 41
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD28 cytoplasmic domain

<400> 4
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210> 5
<211> 112
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD3 zeta domain

<400> 5
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  
Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  
Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      
Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         



<210> 6
<211> 469
<212> PRT
<213> Artificial Sequence


<220> 
<223> SLAMF7-binding CAR, extracellular domain; huLuc63 VH, linker,
      huLuc63 VL, hinge, CH2, CH3

<400> 6
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu 
    50                  55                  60                  
Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Pro Asp Gly Asn Tyr Trp Tyr Phe Asp Val Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
145                 150                 155                 160 
Gln Asp Val Gly Ile Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Val Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val 
            180                 185                 190         
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 
Tyr Ser Ser Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 
Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro 
                245                 250                 255     
Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
305                 310                 315                 320 
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            340                 345                 350         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            420                 425                 430         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 
Leu Ser Leu Gly Lys 
465                 

<210> 7
<211> 153
<212> PRT
<213> Artificial Sequence


<220> 
<223> SLAMF7-binding CAR, intracellular signalling domain; CD28
      cytoplasmic, CD3 zeta

<400> 7
Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        35                  40                  45              
Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    50                  55                  60                  
Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
65                  70                  75                  80  
Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                85                  90                  95      
Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            100                 105                 110         
Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        115                 120                 125             
Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    130                 135                 140                 
Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150             

<210> 8
<211> 650
<212> PRT
<213> Artificial Sequence


<220> 
<223> SLAMF7-binding CAR; huLuc63 VH, linker, huLuc63 VL, hinge, CH2,
      CH3, CD28 tm, CD28 cytoplasmic, CD3 zeta

<400> 8
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu 
    50                  55                  60                  
Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Pro Asp Gly Asn Tyr Trp Tyr Phe Asp Val Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             
Ser Gly Gly Gly Gly Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser 
    130                 135                 140                 
Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Lys Ala Ser 
145                 150                 155                 160 
Gln Asp Val Gly Ile Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys 
                165                 170                 175     
Val Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg His Thr Gly Val 
            180                 185                 190         
Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
        195                 200                 205             
Ile Ser Ser Leu Gln Pro Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 
Tyr Ser Ser Tyr Pro Tyr Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 
Lys Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Pro 
                245                 250                 255     
Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            260                 265                 270         
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        275                 280                 285             
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    290                 295                 300                 
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln Ser 
305                 310                 315                 320 
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                325                 330                 335     
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            340                 345                 350         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        355                 360                 365             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    370                 375                 380                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
385                 390                 395                 400 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                405                 410                 415     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            420                 425                 430         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        435                 440                 445             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    450                 455                 460                 
Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly Val 
465                 470                 475                 480 
Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
                485                 490                 495     
Val Arg Ser Lys Arg Ser Arg Gly Gly His Ser Asp Tyr Met Asn Met 
            500                 505                 510         
Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
        515                 520                 525             
Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
    530                 535                 540                 
Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
545                 550                 555                 560 
Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
                565                 570                 575     
Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
            580                 585                 590         
Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
        595                 600                 605             
Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
    610                 615                 620                 
Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
625                 630                 635                 640 
Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                645                 650 

<210> 9
<211> 225
<212> DNA
<213> Artificial Sequence


<220> 
<223> left IR/DR segment; left inverted repeats which are recognized
      and bound by transposase

<400> 9
cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac     60

tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact    120

ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata    180

attcactgta tcacaattcc agtgggtcag aagtttacat acact                    225


<210> 10
<211> 225
<212> DNA
<213> Artificial Sequence


<220> 
<223> right IR/DR segment; right inverted repeats which are recognized
      and bound by transposase

<400> 10
agtgtatgta aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa     60

tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac    120

tgacctaaga cagggaattt ttactaggat taaatgtcag gaattgtgaa aaagtgagtt    180

taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactg                    225


<210> 11
<211> 4352
<212> DNA
<213> Artificial Sequence


<220> 
<223> SLAMF7-binding CAR integration cassette comprising left IR/DR,
      EF-1 alpha core promoter, Kozak, GMCSF SP, huLuc63 VH, (4GS)3
      linker, huLuc63 VL, IgG4 Hinge, IgG4 CH2CH3 NQ, CD28 tm, CD28
      cytoplasmic, T2A, EGFRt, right IR/DR

<400> 11
cagttgaagt cggaagttta catacactta agttggagtc attaaaactc gtttttcaac       60

tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact      120

ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata      180

attcactgta tcacaattcc agtgggtcag aagtttacat acactaagtt gactgtgcct      240

ttaaacagct tggaaaattc cagaaaatga tgtcatggct ttagaagctt gatatccatg      300

gaattcggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag      360

tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg      420

gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag      480

aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca      540

gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct      600

gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct      660

gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg      720

cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg      780

ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac      840

cggcgcctac ggctagcgcc gccaccatgc tgctgctcgt gacatctctg ctgctgtgcg      900

agctgcccca ccccgccttt ctgctgattc ctgaggtgca gctggtggaa agcggcggag      960

gactggtgca gcctggcgga tctctgagac tgagctgtgc cgccagcggc ttcgacttca     1020

gccggtactg gatgagctgg gtgcgccagg cccctggcaa aggcctggaa tggatcggcg     1080

agatcaaccc cgacagcagc accatcaact acgcccccag cctgaaggac aagttcatca     1140

tcagccggga caacgccaag aacagcctgt acctgcagat gaactccctg cgggccgagg     1200

acaccgccgt gtactattgc gccagacccg acggcaacta ctggtacttc gacgtgtggg     1260

gccagggcac cctcgtgaca gtgtctagcg gaggcggcgg atctggcgga gggggatctg     1320

ggggcggagg ctctgatatc cagatgaccc agagccccag cagcctgtct gccagcgtgg     1380

gcgacagagt gaccatcaca tgcaaggcca gccaggacgt gggaatcgcc gtggcctggt     1440

atcagcagaa acccggcaag gtgcccaagc tgctgatcta ctgggccagc accagacaca     1500

ccggcgtgcc cgatagattt tccggcagcg gctccggcac cgacttcacc ctgacaatca     1560

gctccctgca gcctgaggac gtggccacct actactgcca gcagtacagc agctacccct     1620

acaccttcgg acagggcacc aaggtggaaa tcaaagagtc taagtacgga ccgccttgtc     1680

ctccttgtcc agctcctcct gtggccggac ctagcgtgtt cctgttcccc ccaaagccca     1740

aggacaccct gatgatcagc cggacccccg aagtgacctg cgtggtggtg gatgtgtccc     1800

aggaagatcc cgaggtgcag ttcaattggt acgtggacgg cgtggaagtg cacaacgcca     1860

agaccaagcc cagagaggaa cagttccaga gcacctaccg ggtggtgtcc gtgctgacag     1920

tgctgcacca ggactggctg aacggcaaag agtacaagtg caaggtgtcc aacaagggcc     1980

tgcccagcag catcgagaaa accatcagca aggccaaggg ccagcctcgc gagccccagg     2040

tgtacacact gcctccaagc caggaagaga tgaccaagaa ccaggtgtcc ctgacctgtc     2100

tcgtgaaggg cttctacccc agcgacattg ccgtggaatg ggagagcaac ggccagcccg     2160

agaacaacta caagaccacc ccccctgtgc tggacagcga cggctcattc ttcctgtaca     2220

gcagactgac cgtggacaag agccggtggc aggaaggcaa cgtgttcagc tgcagcgtga     2280

tgcacgaggc cctgcacaac cactacaccc agaagtccct gtctctgagc ctgggcaaga     2340

tgttctgggt gctggtggtc gtgggcggag tgctggcctg ttacagcctg ctcgtgaccg     2400

tggccttcat catcttttgg gtgcgcagca agcggagcag aggcggccac agcgactaca     2460

tgaacatgac ccccagacgg cctggcccca ccagaaagca ctaccagcct tacgcccctc     2520

ccagagactt cgccgcctac cggtccagag tgaagttcag cagaagcgcc gacgcccctg     2580

cctatcagca gggccagaac cagctgtaca acgagctgaa cctgggcaga cgggaagagt     2640

acgacgtgct ggataagcgg agaggccggg accctgagat gggcggcaag cctagaagaa     2700

agaaccccca ggaaggcctg tataacgaac tgcagaaaga caagatggcc gaggcctaca     2760

gcgagatcgg catgaagggc gaaagaaggc ggggcaaggg ccacgatggc ctgtatcagg     2820

gactgagcac cgccaccaag gatacctatg acgcactgca catgcaggcc ctgcccccca     2880

gactcgaggg cggaggcgaa ggcagaggat ctctgctgac atgcggcgac gtggaagaga     2940

acccaggccc cagaatgctg ctgctcgtga caagcctgct gctgtgcgag ctgccccacc     3000

ctgcctttct gctgatcccc cggaaagtgt gcaacggcat cggcatcgga gagttcaagg     3060

acagcctgtc catcaacgcc accaacatca agcacttcaa gaattgcacc agcatcagcg     3120

gcgacctgca catcctgcca gtggccttta gaggcgacag cttcacccac acccccccac     3180

tggatccaca ggaactggat attctgaaaa ccgtaaagga aatcacaggg tttttgctga     3240

ttcaggcttg gcctgaaaac aggacggacc tccatgcctt tgagaaccta gaaatcatac     3300

gcggcaggac caagcaacat ggtcagtttt ctcttgcagt cgtcagcctg aacataacat     3360

ccttgggatt acgctccctc aaggagataa gtgatggaga tgtgataatt tcaggaaaca     3420

aaaatttgtg ctatgcaaat acaataaact ggaaaaaact gtttgggacc tccggtcaga     3480

aaaccaaaat tataagcaac agaggtgaaa acagctgcaa ggccacaggc caggtctgcc     3540

atgccttgtg ctcccccgag ggctgctggg gcccggagcc cagggactgc gtctcttgcc     3600

ggaatgtcag ccgaggcagg gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa     3660

gggagtttgt ggagaactct gagtgcatac agtgccaccc agagtgcctg cctcaggcca     3720

tgaacatcac ctgcacagga cggggaccag acaactgtat ccagtgtgcc cactacattg     3780

acggccccca ctgcgtcaag acctgcccgg caggagtcat gggagaaaac aacaccctgg     3840

tctggaagta cgcagacgcc ggccatgtgt gccacctgtg ccatccaaac tgcacctacg     3900

gatgcactgg gccaggtctt gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg     3960

ccactgggat ggtgggggcc ctcctcttgc tgctggtggt ggccctgggg atcggcctct     4020

tcatgtgagc ggccgctcta gatggccaga tctagcttgt ggaaggctac tcgaaatgtt     4080

tgacccaagt taaacaattt aaaggcaatg ctaccaaata ctaattgagt gtatgtaaac     4140

ttctgaccca ctgggaatgt gatgaaagaa ataaaagctg aaatgaatca ttctctctac     4200

tattattctg atatttcaca ttcttaaaat aaagtggtga tcctaactga cctaagacag     4260

ggaattttta ctaggattaa atgtcaggaa ttgtgaaaaa gtgagtttaa atgtatttgg     4320

ctaaggtgta tgtaaacttc cgacttcaac tg                                   4352


<210> 12
<211> 4611
<212> DNA
<213> Artificial Sequence


<220> 
<223> SLAMF7 CAR minicircle DNA

<400> 12
gccttggtca aattgggtat acccatttgg gcctagtcta gccggcatgg cgcattacag       60

caatacgcaa tttaaatgcg cctagcgcat tttcccgacc ttaatgcgcc tcgcgctgta      120

gcctcacgcc cacatatgtg cagatctgaa gaagaagaag aagaagaaga agaagaagaa      180

gaagaagaag aagaagaaga agaagaagaa gtttctcgga tccctataca gttgaagtcg      240

gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta ctccacaaat      300

ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt gtgcatgaca      360

caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat tcactgtatc      420

acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt aaacagcttg      480

gaaaattcca gaaaatgatg tcatggcttt agaagcttga tatccatgga attcggatct      540

gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt      600

tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg      660

aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa      720

gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga      780

agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc      840

cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg      900

ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga      960

gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg     1020

tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgg     1080

ctagcgccgc caccatgctg ctgctcgtga catctctgct gctgtgcgag ctgccccacc     1140

ccgcctttct gctgattcct gaggtgcagc tggtggaaag cggcggagga ctggtgcagc     1200

ctggcggatc tctgagactg agctgtgccg ccagcggctt cgacttcagc cggtactgga     1260

tgagctgggt gcgccaggcc cctggcaaag gcctggaatg gatcggcgag atcaaccccg     1320

acagcagcac catcaactac gcccccagcc tgaaggacaa gttcatcatc agccgggaca     1380

acgccaagaa cagcctgtac ctgcagatga actccctgcg ggccgaggac accgccgtgt     1440

actattgcgc cagacccgac ggcaactact ggtacttcga cgtgtggggc cagggcaccc     1500

tcgtgacagt gtctagcgga ggcggcggat ctggcggagg gggatctggg ggcggaggct     1560

ctgatatcca gatgacccag agccccagca gcctgtctgc cagcgtgggc gacagagtga     1620

ccatcacatg caaggccagc caggacgtgg gaatcgccgt ggcctggtat cagcagaaac     1680

ccggcaaggt gcccaagctg ctgatctact gggccagcac cagacacacc ggcgtgcccg     1740

atagattttc cggcagcggc tccggcaccg acttcaccct gacaatcagc tccctgcagc     1800

ctgaggacgt ggccacctac tactgccagc agtacagcag ctacccctac accttcggac     1860

agggcaccaa ggtggaaatc aaagagtcta agtacggacc gccttgtcct ccttgtccag     1920

ctcctcctgt ggccggacct agcgtgttcc tgttcccccc aaagcccaag gacaccctga     1980

tgatcagccg gacccccgaa gtgacctgcg tggtggtgga tgtgtcccag gaagatcccg     2040

aggtgcagtt caattggtac gtggacggcg tggaagtgca caacgccaag accaagccca     2100

gagaggaaca gttccagagc acctaccggg tggtgtccgt gctgacagtg ctgcaccagg     2160

actggctgaa cggcaaagag tacaagtgca aggtgtccaa caagggcctg cccagcagca     2220

tcgagaaaac catcagcaag gccaagggcc agcctcgcga gccccaggtg tacacactgc     2280

ctccaagcca ggaagagatg accaagaacc aggtgtccct gacctgtctc gtgaagggct     2340

tctaccccag cgacattgcc gtggaatggg agagcaacgg ccagcccgag aacaactaca     2400

agaccacccc ccctgtgctg gacagcgacg gctcattctt cctgtacagc agactgaccg     2460

tggacaagag ccggtggcag gaaggcaacg tgttcagctg cagcgtgatg cacgaggccc     2520

tgcacaacca ctacacccag aagtccctgt ctctgagcct gggcaagatg ttctgggtgc     2580

tggtggtcgt gggcggagtg ctggcctgtt acagcctgct cgtgaccgtg gccttcatca     2640

tcttttgggt gcgcagcaag cggagcagag gcggccacag cgactacatg aacatgaccc     2700

ccagacggcc tggccccacc agaaagcact accagcctta cgcccctccc agagacttcg     2760

ccgcctaccg gtccagagtg aagttcagca gaagcgccga cgcccctgcc tatcagcagg     2820

gccagaacca gctgtacaac gagctgaacc tgggcagacg ggaagagtac gacgtgctgg     2880

ataagcggag aggccgggac cctgagatgg gcggcaagcc tagaagaaag aacccccagg     2940

aaggcctgta taacgaactg cagaaagaca agatggccga ggcctacagc gagatcggca     3000

tgaagggcga aagaaggcgg ggcaagggcc acgatggcct gtatcaggga ctgagcaccg     3060

ccaccaagga tacctatgac gcactgcaca tgcaggccct gccccccaga ctcgagggcg     3120

gaggcgaagg cagaggatct ctgctgacat gcggcgacgt ggaagagaac ccaggcccca     3180

gaatgctgct gctcgtgaca agcctgctgc tgtgcgagct gccccaccct gcctttctgc     3240

tgatcccccg gaaagtgtgc aacggcatcg gcatcggaga gttcaaggac agcctgtcca     3300

tcaacgccac caacatcaag cacttcaaga attgcaccag catcagcggc gacctgcaca     3360

tcctgccagt ggcctttaga ggcgacagct tcacccacac ccccccactg gatccacagg     3420

aactggatat tctgaaaacc gtaaaggaaa tcacagggtt tttgctgatt caggcttggc     3480

ctgaaaacag gacggacctc catgcctttg agaacctaga aatcatacgc ggcaggacca     3540

agcaacatgg tcagttttct cttgcagtcg tcagcctgaa cataacatcc ttgggattac     3600

gctccctcaa ggagataagt gatggagatg tgataatttc aggaaacaaa aatttgtgct     3660

atgcaaatac aataaactgg aaaaaactgt ttgggacctc cggtcagaaa accaaaatta     3720

taagcaacag aggtgaaaac agctgcaagg ccacaggcca ggtctgccat gccttgtgct     3780

cccccgaggg ctgctggggc ccggagccca gggactgcgt ctcttgccgg aatgtcagcc     3840

gaggcaggga atgcgtggac aagtgcaacc ttctggaggg tgagccaagg gagtttgtgg     3900

agaactctga gtgcatacag tgccacccag agtgcctgcc tcaggccatg aacatcacct     3960

gcacaggacg gggaccagac aactgtatcc agtgtgccca ctacattgac ggcccccact     4020

gcgtcaagac ctgcccggca ggagtcatgg gagaaaacaa caccctggtc tggaagtacg     4080

cagacgccgg ccatgtgtgc cacctgtgcc atccaaactg cacctacgga tgcactgggc     4140

caggtcttga aggctgtcca acgaatgggc ctaagatccc gtccatcgcc actgggatgg     4200

tgggggccct cctcttgctg ctggtggtgg ccctggggat cggcctcttc atgtgagcgg     4260

ccgctctaga tggccagatc tagcttgtgg aaggctactc gaaatgtttg acccaagtta     4320

aacaatttaa aggcaatgct accaaatact aattgagtgt atgtaaactt ctgacccact     4380

gggaatgtga tgaaagaaat aaaagctgaa atgaatcatt ctctctacta ttattctgat     4440

atttcacatt cttaaaataa agtggtgatc ctaactgacc taagacaggg aatttttact     4500

aggattaaat gtcaggaatt gtgaaaaagt gagtttaaat gtatttggct aaggtgtatg     4560

taaacttccg acttcaactg tatagggatc ctctagctag agtcgactgc a              4611


<210> 13
<211> 340
<212> PRT
<213> Artificial Sequence


<220> 
<223> SB transposase

<400> 13
Met Gly Lys Ser Lys Glu Ile Ser Gln Asp Leu Arg Lys Arg Ile Val 
1               5                   10                  15      
Asp Leu His Lys Ser Gly Ser Ser Leu Gly Ala Ile Ser Lys Arg Leu 
            20                  25                  30          
Ala Val Pro Arg Ser Ser Val Gln Thr Ile Val Arg Lys Tyr Lys His 
        35                  40                  45              
His Gly Thr Thr Gln Pro Ser Tyr Arg Ser Gly Arg Arg Arg Val Leu 
    50                  55                  60                  
Ser Pro Arg Asp Glu Arg Thr Leu Val Arg Lys Val Gln Ile Asn Pro 
65                  70                  75                  80  
Arg Thr Thr Ala Lys Asp Leu Val Lys Met Leu Glu Glu Thr Gly Thr 
                85                  90                  95      
Lys Val Ser Ile Ser Thr Val Lys Arg Val Leu Tyr Arg His Asn Leu 
            100                 105                 110         
Lys Gly His Ser Ala Arg Lys Lys Pro Leu Leu Gln Asn Arg His Lys 
        115                 120                 125             
Lys Ala Arg Leu Arg Phe Ala Thr Ala His Gly Asp Lys Asp Arg Thr 
    130                 135                 140                 
Phe Trp Arg Asn Val Leu Trp Ser Asp Glu Thr Lys Ile Glu Leu Phe 
145                 150                 155                 160 
Gly His Asn Asp His Arg Tyr Val Trp Arg Lys Lys Gly Glu Ala Cys 
                165                 170                 175     
Lys Pro Lys Asn Thr Ile Pro Thr Val Lys His Gly Gly Gly Ser Ile 
            180                 185                 190         
Met Leu Trp Gly Cys Phe Ala Ala Gly Gly Thr Gly Ala Leu His Lys 
        195                 200                 205             
Ile Asp Gly Ile Met Asp Ala Val Gln Tyr Val Asp Ile Leu Lys Gln 
    210                 215                 220                 
His Leu Lys Thr Ser Val Arg Lys Leu Lys Leu Gly Arg Lys Trp Val 
225                 230                 235                 240 
Phe Gln His Asp Asn Asp Pro Lys His Thr Ser Lys Val Val Ala Lys 
                245                 250                 255     
Trp Leu Lys Asp Asn Lys Val Lys Val Leu Glu Trp Pro Ser Gln Ser 
            260                 265                 270         
Pro Asp Leu Asn Pro Ile Glu Asn Leu Trp Ala Glu Leu Lys Lys Arg 
        275                 280                 285             
Val Arg Ala Arg Arg Pro Thr Asn Leu Thr Gln Leu His Gln Leu Cys 
    290                 295                 300                 
Gln Glu Glu Trp Ala Lys Ile His Pro Asn Tyr Cys Gly Lys Leu Val 
305                 310                 315                 320 
Glu Gly Tyr Pro Lys Arg Leu Thr Gln Val Lys Gln Phe Lys Gly Asn 
                325                 330                 335     
Ala Thr Lys Tyr 
            340 

<210> 14
<211> 6637
<212> DNA
<213> Artificial Sequence


<220> 
<223> pcGlobin2-SB100X plasmid

<400> 14
gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg       60

ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg      120

cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc      180

ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt      240

gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata      300

tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc      360

cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc      420

attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt      480

atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt      540

atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca      600

tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg      660

actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc      720

aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg      780

gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca      840

ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggcacg      900

agctggactt taagcaagca cctaaggaca tccaaaagcg taaacggatc caccatgggc      960

aaatccaagg agatctccca ggacctgagg aaaagaatcg tggacctgca caaatctggc     1020

tcttctctgg gcgccatctc taaaagactg gccgtgccta ggtccagcgt gcagaccatt     1080

gtgcggaaat acaaacacca cggaaccaca cagccatctt accgctccgg acggcggaga     1140

gtgctgtccc ctagagacga gaggaccctc gtgagaaaag tgcagatcaa ccctagaaca     1200

accgccaaag acctggtgaa aatgctggag gagaccggca ccaaggtgtc catctctacc     1260

gtgaagcgcg tgctgtaccg gcacaacctg aagggacact ccgcccggaa gaaacctctg     1320

ctgcagaata ggcacaagaa agcccggctg agattcgcca ccgcccacgg cgataaggat     1380

agaacctttt ggcgcaacgt gctgtggagc gacgagacaa aaatcgagct gttcgggcac     1440

aacgatcacc gctacgtgtg gcgcaaaaag ggagaggcct gtaagccaaa aaacaccatc     1500

cccaccgtga aacacggcgg aggctccatt atgctgtggg gctgctttgc cgccggcgga     1560

accggagccc tgcacaaaat cgacggcatc atggatgccg tgcagtacgt ggacatcctg     1620

aaacagcacc tgaaaacctc tgtgagaaaa ctgaaactgg gccgcaaatg ggtgttccag     1680

cacgacaatg accctaagca cacatccaaa gtggtggcca aatggctgaa agacaacaaa     1740

gtgaaagtgc tggagtggcc ttcccagtcc cccgatctga acccaattga gaacctgtgg     1800

gccgagctga agaaaagagt gcgggccaga cggcctacaa acctgacaca gctgcaccag     1860

ctgtgtcagg aggagtgggc caaaatccac cccaactact gtggcaagct ggtggaggga     1920

tacccaaaac ggctgaccca agtgaaacag ttcaagggca acgccaccaa gtactgagaa     1980

ttctgcagat atccatcaca ctggcggccg ctcgagtctc atcgccaatg aacgtcaggt     2040

gttttggaga aggtcacacc gcgcggagat gttcaaacaa agatctaact cttttgcaac     2100

aagatcaata aaataatgtt aaaagcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaatcta     2160

gaatcgatgc gcgctagccc gggccctatt ctatagtgtc acctaaatgc tagagctcgc     2220

tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg     2280

ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt     2340

gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc     2400

aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct     2460

tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc     2520

gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc     2580

ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc     2640

cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc     2700

gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg     2760

gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact     2820

ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt     2880

tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt     2940

ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc     3000

aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag     3060

gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc     3120

cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa     3180

ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt     3240

gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca     3300

ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat     3360

tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac     3420

agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc     3480

tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc     3540

tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag     3600

cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc     3660

ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg     3720

atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc     3780

ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc     3840

cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga     3900

cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca     3960

tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg     4020

atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg     4080

ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg     4140

gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga     4200

ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg     4260

gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat     4320

tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt     4380

tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg     4440

tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg     4500

aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc     4560

ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt     4620

ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg     4680

cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt     4740

tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc     4800

aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa     4860

aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa     4920

tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc     4980

ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc     5040

cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag     5100

ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga     5160

ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc     5220

gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac     5280

agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg     5340

cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca     5400

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa     5460

aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa     5520

ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt     5580

aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag     5640

ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat     5700

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc     5760

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa     5820

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca     5880

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa     5940

cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt     6000

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc     6060

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact     6120

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc     6180

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg     6240

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct     6300

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc     6360

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag     6420

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac     6480

acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg     6540

ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt     6600

tccgcgcaca tttccccgaa aagtgccacc tgacgtc                              6637


<210> 15
<211> 335
<212> PRT
<213> Artificial Sequence


<220> 
<223> EGFRt; truncated EGFR tag to facilitate testing for CAR+ cells
      and as potential depletion marker (suicide switch)

<400> 15
Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu 
1               5                   10                  15      
Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile 
            20                  25                  30          
Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe 
        35                  40                  45              
Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr 
    50                  55                  60                  
Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn 
65                  70                  75                  80  
Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg 
                85                  90                  95      
Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile 
            100                 105                 110         
Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val 
        115                 120                 125             
Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp 
    130                 135                 140                 
Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn 
145                 150                 155                 160 
Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu 
                165                 170                 175     
Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser 
            180                 185                 190         
Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu 
        195                 200                 205             
Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln 
    210                 215                 220                 
Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly 
225                 230                 235                 240 
Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro 
                245                 250                 255     
His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr 
            260                 265                 270         
Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His 
        275                 280                 285             
Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro 
    290                 295                 300                 
Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala 
305                 310                 315                 320 
Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met 
                325                 330                 335 

<210> 16
<211> 1008
<212> DNA
<213> Artificial Sequence


<220> 
<223> EGFRt; truncated EGFR tag to facilitate testing for CAR+ cells
      and as potential depletion marker (suicide switch)

<400> 16
cggaaagtgt gcaacggcat cggcatcgga gagttcaagg acagcctgtc catcaacgcc      60

accaacatca agcacttcaa gaattgcacc agcatcagcg gcgacctgca catcctgcca     120

gtggccttta gaggcgacag cttcacccac acccccccac tggatccaca ggaactggat     180

attctgaaaa ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac     240

aggacggacc tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat     300

ggtcagtttt ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc     360

aaggagataa gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat     420

acaataaact ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac     480

agaggtgaaa acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag     540

ggctgctggg gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg     600

gaatgcgtgg acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct     660

gagtgcatac agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga     720

cggggaccag acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag     780

acctgcccgg caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc     840

ggccatgtgt gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt     900

gaaggctgtc caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc     960

ctcctcttgc tgctggtggt ggccctgggg atcggcctct tcatgtga                 1008


<210> 17
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> huLuc63 VH: heavy chain of variable fragment derived from
      SLAMF7-binding antibody huLuc63

<400> 17
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asp Phe Ser Arg Tyr 
            20                  25                  30          
Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              
Gly Glu Ile Asn Pro Asp Ser Ser Thr Ile Asn Tyr Ala Pro Ser Leu 
    50                  55                  60                  
Lys Asp Lys Phe Ile Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Pro Asp Gly Asn Tyr Trp Tyr Phe Asp Val Trp Gly Gln Gly 
            100                 105                 110         
Thr Leu Val Thr Val Ser Ser 
        115                 

<210> 18
<211> 357
<212> DNA
<213> Artificial Sequence


<220> 
<223> huLuc63 VH: heavy chain of variable fragment derived from
      SLAMF7-binding antibody huLuc63

<400> 18
gaggtgcagc tggtggaaag cggcggagga ctggtgcagc ctggcggatc tctgagactg      60

agctgtgccg ccagcggctt cgacttcagc cggtactgga tgagctgggt gcgccaggcc     120

cctggcaaag gcctggaatg gatcggcgag atcaaccccg acagcagcac catcaactac     180

gcccccagcc tgaaggacaa gttcatcatc agccgggaca acgccaagaa cagcctgtac     240

ctgcagatga actccctgcg ggccgaggac accgccgtgt actattgcgc cagacccgac     300

ggcaactact ggtacttcga cgtgtggggc cagggcaccc tcgtgacagt gtctagc        357


<210> 19
<211> 107
<212> PRT
<213> Artificial Sequence


<220> 
<223> huLuc63 VL: light chain of variable fragment derived from
      SLAMF7-binding antibody huLuc63

<400> 19
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Val Gly Ile Ala 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Val Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Trp Ala Ser Thr Arg His Thr Gly Val Pro Asp Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Val Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Ser Tyr Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         

<210> 20
<211> 321
<212> DNA
<213> Artificial Sequence


<220> 
<223> huLuc63 VL: light chain of variable fragment derived from
      SLAMF7-binding antibody huLuc63

<400> 20
gatatccaga tgacccagag ccccagcagc ctgtctgcca gcgtgggcga cagagtgacc      60

atcacatgca aggccagcca ggacgtggga atcgccgtgg cctggtatca gcagaaaccc     120

ggcaaggtgc ccaagctgct gatctactgg gccagcacca gacacaccgg cgtgcccgat     180

agattttccg gcagcggctc cggcaccgac ttcaccctga caatcagctc cctgcagcct     240

gaggacgtgg ccacctacta ctgccagcag tacagcagct acccctacac cttcggacag     300

ggcaccaagg tggaaatcaa a                                               321


<210> 21
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide 1

<400> 21
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro 
1               5                   10                  15      
Ala Phe Leu Leu Ile Pro 
            20          

<210> 22
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> linker

<400> 22
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  

<210> 23
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> T2A sequence

<400> 23
Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      
Val Glu Glu Asn Pro Gly Pro Arg Met 
            20                  25  

<210> 24
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide 2

<400> 24
Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      
Phe Leu Leu Ile Pro 
            20      

<210> 25
<211> 42
<212> PRT
<213> Artificial Sequence


<220> 
<223> 4-1BB domain

<400> 25
Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      
Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          
Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          

<210> 26
<211> 126
<212> DNA
<213> Artificial Sequence


<220> 
<223> 4-1BB domain

<400> 26
aagcggggca gaaagaagct gctgtatatc ttcaagcagc ccttcatgcg gcccgtgcag     60

accacacagg aagaggacgg ctgctcctgc cggttccccg aggaagaaga aggcggctgc    120

gagctg                                                               126


