                         SEQUENCE LISTING

<110>  SHANDONG BOAN BIOTECHNOLOGY CO., LTD.
 
<120>  Anti-GPC3 antibody, anti-GPC3 chimeric antigen receptor and 
       GPC3/CD3 bispecific antibody

<130>  BA11

<150>  63/047,239
<151>  2020-07-01

<150>  63/063,550
<151>  2020-08-10

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR1

<400>  1

Gly Phe Ser Leu Ser Thr Tyr Trp Met Thr 
1               5                   10  


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR2

<400>  2

Ile Ile Ser Pro Ala Gly Ser Ala Tyr Tyr Ala Ser Trp Ala Lys Gly 
1               5                   10                  15      


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR3

<400>  3

Ala Gly Gly Gly Gly Met Asp Pro 
1               5               


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR1

<400>  4

Gln Ser Ser Gln Ser Ile Val Lys Asn Tyr Leu Ser 
1               5                   10          


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR2

<400>  5

Lys Ala Ser Thr Leu Pro Ser 
1               5           


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR3

<400>  6

Ala Ala Ser Tyr Ser Asp Asn Ile Tyr Val 
1               5                   10  


<210>  7
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH1

<400>  7

Gln Cys Gln Ser Val Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Ser Leu Ser Thr 
            20                  25                  30          


Tyr Trp Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ile Ile Ser Pro Ala Gly Ser Ala Tyr Tyr Ala Ser Trp Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Ala Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Phe Cys Ala 
                85                  90                  95      


Gly Gly Gly Gly Met Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  8
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL1

<400>  8

Asp Ile Val Leu Thr Gln Ser Pro Ser Ala Met Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Ile Val Lys Asn 
            20                  25                  30          


Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly Lys Pro Pro Lys Arg Leu 
        35                  40                  45              


Ile Tyr Lys Ala Ser Thr Leu Pro Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Ala Ser Tyr Ser Asp Asn 
                85                  90                  95      


Ile Tyr Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 


<210>  9
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL3

<400>  9

Asp Pro Val Leu Thr Gln Ser Pro Ser Ala Met Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Ile Val Lys Asn 
            20                  25                  30          


Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu 
        35                  40                  45              


Ile Tyr Lys Ala Ser Thr Leu Pro Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Ala Ser Tyr Ser Asp Asn 
                85                  90                  95      


Ile Tyr Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 


<210>  10
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3-VH

<400>  10

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Lys Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Asn Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Ile Ser Tyr Trp 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  11
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3-VL

<400>  11

Gln Thr Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Ser Gly 
            20                  25                  30          


Tyr Tyr Pro Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Lys Phe Leu Ala Pro Gly Thr Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Val 
65                  70                  75                  80  


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


Arg Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  12
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 OPT1a3b-VH

<400>  12

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Asn Leu Arg Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Trp Gly Asn Ser Tyr Ile Ser Tyr Trp 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 


<210>  13
<211>  110
<212>  PRT
<213>  Homo sapiens

<400>  13

Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  14
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD3 OPT1a3b2b1-VH

<400>  14

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Thr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Ala Tyr Leu Gln Met Asn Asn Leu Arg Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Trp Gly Asn Ser Tyr Ile Ser Tyr Trp 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
        115                 120                 125 


<210>  15
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CH2 of human IgGl heavy chain

<400>  15

Ala Pro Glu Ala Ala Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  16
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clone 40-VH

<400>  16

Gln Cys Gln Ser Val Glu Glu Ser Gly Gly Arg Leu Val Thr Pro Gly 
1               5                   10                  15      


Thr Pro Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Thr 
            20                  25                  30          


Tyr Trp Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ile Ile Ser Pro Ala Gly Ser Ala Tyr Tyr Ala Ser Trp Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Thr Ser Ser Ala Thr Val Asp Leu 
65                  70                  75                  80  


Lys Met Thr Ser Leu Thr Thr Ala Asp Thr Ala Thr Tyr Phe Cys Ala 
                85                  90                  95      


Gly Gly Gly Gly Met Asp Pro Trp Gly Pro Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  17
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clone 40-VL

<400>  17

Asp Pro Val Leu Thr Gln Thr Pro Ser Ser Val Ser Ala Ala Val Gly 
1               5                   10                  15      


Gly Thr Val Ser Ile Ser Cys Gln Ser Ser Gln Ser Ile Val Lys Asn 
            20                  25                  30          


Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Arg Leu 
        35                  40                  45              


Ile Tyr Lys Ala Ser Thr Leu Pro Ser Gly Val Pro Ser Arg Phe Lys 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asp Leu Glu 
65                  70                  75                  80  


Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Ala Ala Ser Tyr Ser Asp Asn 
                85                  90                  95      


Ile Tyr Val Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 


<210>  18
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH2

<400>  18

Gln Cys Gln Ser Val Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Ser Leu Ser Thr 
            20                  25                  30          


Tyr Trp Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ile Ile Ser Pro Ala Gly Ser Ala Tyr Tyr Ala Ser Trp Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Thr Asn Ser Ala Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr Tyr Phe Cys Ala 
                85                  90                  95      


Gly Gly Gly Gly Met Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  19
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH3

<400>  19

Gln Cys Gln Ser Val Glu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly 
1               5                   10                  15      


Gly Ser Leu Arg Leu Ser Cys Thr Val Ser Gly Phe Ser Leu Ser Thr 
            20                  25                  30          


Tyr Trp Met Thr Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Ile Ile Ser Pro Ala Gly Ser Ala Tyr Tyr Ala Ser Trp Ala 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Lys Thr Asn Ser Ala Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Thr Tyr Phe Cys Ala 
                85                  90                  95      


Gly Gly Gly Gly Met Asp Pro Trp Gly Gln Gly Thr Leu Val Thr Val 
            100                 105                 110         


Ser Ser 
        


<210>  20
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL2

<400>  20

Asp Pro Val Leu Thr Gln Ser Pro Ser Ala Met Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Gln Ser Ser Gln Ser Ile Val Lys Asn 
            20                  25                  30          


Tyr Leu Ser Trp Phe Gln Gln Lys Pro Gly Lys Pro Pro Lys Arg Leu 
        35                  40                  45              


Ile Tyr Lys Ala Ser Thr Leu Pro Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Ala Ala Ser Tyr Ser Asp Asn 
                85                  90                  95      


Ile Tyr Val Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105                 


<210>  21
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR1

<400>  21

Ser Thr Tyr Asn Met Gly 
1               5       


<210>  22
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR2

<400>  22

Thr Ile Ser Ser Ser Thr Gly Asn Thr Tyr Tyr Ala Thr Trp Ala Lys 
1               5                   10                  15      


Gly 
    


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCDR3

<400>  23

Val Asn Asn Met Gly Asp Ile 
1               5           


<210>  24
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR1

<400>  24

Gln Ala Ser Glu Ser Val Tyr Lys Asp Asn Arg Leu Ala 
1               5                   10              


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR2

<400>  25

Leu Ala Ser Thr Leu Ala Ser 
1               5           


<210>  26
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LCDR3

<400>  26

Gly Gly Tyr Lys Asp Ser Leu Phe Asp Gly Phe Pro 
1               5                   10          


<210>  27
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clone 2-VH

<400>  27

Gln Cys Gln Ser Val Glu Glu Ser Gly Gly Arg Leu Val Thr Pro Gly 
1               5                   10                  15      


Thr Pro Leu Thr Leu Thr Cys Thr Val Ser Gly Phe Ser Leu Ser Thr 
            20                  25                  30          


Tyr Asn Met Gly Trp Ile Arg Gln Ala Pro Gly Glu Gly Leu Glu Tyr 
        35                  40                  45              


Ile Gly Thr Ile Ser Ser Ser Thr Gly Asn Thr Tyr Tyr Ala Thr Trp 
    50                  55                  60                  


Ala Lys Gly Arg Phe Thr Ile Ser Lys Thr Ser Ser Thr Thr Val Asp 
65                  70                  75                  80  


Leu Lys Ile Thr Asn Leu Thr Thr Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Val Arg Val Asn Asn Met Gly Asp Ile Trp Gly Pro Gly Thr Leu Val 
            100                 105                 110         


Thr Val Ser Leu 
        115     


<210>  28
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clone 2- VL

<400>  28

Ala Ile Val Met Thr Gln Thr Pro Ser Ser Lys Ser Val Ala Val Gly 
1               5                   10                  15      


Asp Thr Val Thr Ile Asn Cys Gln Ala Ser Glu Ser Val Tyr Lys Asp 
            20                  25                  30          


Asn Arg Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Ala Pro Lys Leu 
        35                  40                  45              


Leu Ile Tyr Leu Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Lys Gly Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Asp Val 
65                  70                  75                  80  


Val Cys Asp Asp Ala Ala Thr Tyr Tyr Cys Gly Gly Tyr Lys Asp Ser 
                85                  90                  95      


Leu Phe Asp Gly Phe Pro Phe Gly Gly Gly Thr Glu Val Val Val Lys 
            100                 105                 110         


<210>  29
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  29

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  30
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  30

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr 
        35                  40                  45          


<210>  31
<211>  22
<212>  PRT
<213>  Homo sapiens

<400>  31

Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu 
1               5                   10                  15      


Val Ile Thr Leu Tyr Cys 
            20          


<210>  32
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  32

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  33
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  33

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  34
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  34

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  35
<211>  386
<212>  PRT
<213>  Homo sapiens

<400>  35

Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala 
1               5                   10                  15      


Phe Leu Leu Ile Pro Ala Cys Gly Ala Asp Ser Tyr Glu Met Glu Glu 
            20                  25                  30          


Asp Gly Val Arg Lys Cys Lys Lys Cys Glu Gly Pro Cys Arg Lys Val 
        35                  40                  45              


Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn 
    50                  55                  60                  


Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp 
65                  70                  75                  80  


Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr 
                85                  90                  95      


Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu 
            100                 105                 110         


Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp 
        115                 120                 125             


Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln 
    130                 135                 140                 


His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu 
145                 150                 155                 160 


Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser 
                165                 170                 175     


Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu 
            180                 185                 190         


Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu 
        195                 200                 205             


Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro 
    210                 215                 220                 


Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn 
225                 230                 235                 240 


Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly 
                245                 250                 255     


Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro 
            260                 265                 270         


Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro 
        275                 280                 285             


Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val 
    290                 295                 300                 


Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp 
305                 310                 315                 320 


Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys 
                325                 330                 335     


Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly 
            340                 345                 350         


Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu 
        355                 360                 365             


Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met Arg Arg Arg His 
    370                 375                 380                 


Ile Val 
385     


<210>  36
<211>  507
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GC33 CAR

<400>  36

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Pro Gly 
1               5                   10                  15      


Glu Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val His Ser 
            20                  25                  30          


Asn Ala Asn Thr Tyr Leu His Trp Tyr Leu Gln Lys Pro Gly Gln Ser 
        35                  40                  45              


Pro Gln Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Ser Gln Asn 
                85                  90                  95      


Thr His Val Pro Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
    130                 135                 140                 


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr 
145                 150                 155                 160 


Glu Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
                165                 170                 175     


Gly Ala Leu Asp Pro Lys Thr Gly Asp Thr Ala Tyr Ser Gln Lys Phe 
            180                 185                 190         


Lys Gly Arg Val Thr Leu Thr Ala Asp Glu Ser Thr Ser Thr Ala Tyr 
        195                 200                 205             


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
    210                 215                 220                 


Thr Arg Phe Tyr Ser Tyr Thr Tyr Trp Gly Gln Gly Thr Leu Val Thr 
225                 230                 235                 240 


Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro 
                245                 250                 255     


Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro 
            260                 265                 270         


Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        275                 280                 285             


Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
    290                 295                 300                 


Ser Leu Val Ile Thr Leu Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu 
305                 310                 315                 320 


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
                325                 330                 335     


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
            340                 345                 350         


Ser Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
        355                 360                 365             


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
    370                 375                 380                 


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
385                 390                 395                 400 


Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr 
                405                 410                 415     


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
            420                 425                 430         


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
        435                 440                 445             


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
    450                 455                 460                 


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
465                 470                 475                 480 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
                485                 490                 495     


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            500                 505         


