﻿                         序列表

<110>  浙江工业大学

<120>  一种草铵膦脱氢酶突变体、基因工程菌及一锅法多酶同步定向进化方法

<160>  34

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  445
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  1
Met Ile Glu Ser Val Glu Ser Phe Leu Ala Arg Leu Lys Lys Arg Asp 
1               5                   10                  15      
Pro Asp Gln Pro Glu Phe His Gln Ala Val Glu Glu Val Leu Arg Ser 
            20                  25                  30          
Leu Trp Pro Phe Leu Glu Ala Asn Pro His Tyr Leu Thr Ser Gly Ile 
        35                  40                  45              
Leu Glu Arg Ile Cys Glu Pro Glu Arg Ala Val Val Phe Arg Val Ser 
    50                  55                  60                  
Trp Val Asp Asp His Gly Lys Val Gln Val Asn Arg Gly Phe Arg Ile 
65                  70                  75                  80  
Gln Met Asn Ser Ala Ile Gly Pro Tyr Lys Gly Gly Leu Arg Phe His 
                85                  90                  95      
Pro Ser Val Asn Leu Gly Val Leu Lys Phe Leu Ala Phe Glu Gln Thr 
            100                 105                 110         
Phe Lys Asn Ser Leu Thr Ser Leu Pro Met Gly Gly Gly Lys Gly Gly 
        115                 120                 125             
Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Ala Glu Val Met Arg Phe 
    130                 135                 140                 
Cys Gln Ala Phe Met Ser Glu Leu Tyr Arg His Ile Gly Ala Asp Val 
145                 150                 155                 160 
Asp Val Pro Gly Gly Asp Ile Gly Val Gly Ala Arg Glu Ile Gly Phe 
                165                 170                 175     
Leu Phe Gly Gln Tyr Lys Arg Leu Ser Asn Gln Phe Thr Ser Val Leu 
            180                 185                 190         
Thr Gly Lys Gly Pro Ser Tyr Gly Gly Ser Leu Ile Lys Pro Glu Ala 
        195                 200                 205             
Thr Gly Phe Gly Cys Val Tyr Phe Ala Glu Glu Met Leu Lys Arg Arg 
    210                 215                 220                 
Gly Glu Thr Val Glu Gly Lys Arg Val Ala Ile Ser Gly Ser Gly Asn 
225                 230                 235                 240 
Val Ala Gln Tyr Ala Ala Arg Lys Val Met Asp Leu Gly Gly Lys Val 
                245                 250                 255     
Ile Ser Leu Ser Asp Ser Glu Gly Thr Leu Tyr Cys Glu Ser Gly Leu 
            260                 265                 270         
Thr Glu Ala Gln Trp Gln Ala Val Leu Glu Leu Lys Asn Val Gln Arg 
        275                 280                 285             
Gly Arg Ile Ser Glu Leu Ala Ala Arg Phe Gly Leu Glu Phe Arg Ala 
    290                 295                 300                 
Gly Gln His Pro Trp Gly Leu Ser Cys Asp Ile Ala Leu Pro Cys Ala 
305                 310                 315                 320 
Thr Gln Asn Glu Leu Asp Ala Glu Ala Ala Arg Ala Leu Leu Arg Asn 
                325                 330                 335     
Gly Cys Ile Cys Val Ala Glu Gly Ala Asn Met Pro Thr Thr Leu Glu 
            340                 345                 350         
Ala Val Asp Leu Phe Ile Glu Ala Gly Ile Leu Phe Ala Pro Gly Lys 
        355                 360                 365             
Ala Ser Asn Ala Gly Gly Val Ala Val Ser Gly Leu Glu Met Ser Gln 
    370                 375                 380                 
Asn Ala Met Arg Leu Leu Trp Thr Gly Gly Glu Val Asp Ser Lys Leu 
385                 390                 395                 400 
His Ala Ile Met Gln Ser Ile His His Ala Cys Val His Tyr Gly Glu 
                405                 410                 415     
Glu Asn Gly Gln Val Asn Tyr Val Lys Gly Ala Asn Ile Ala Gly Phe 
            420                 425                 430         
Val Lys Val Ala Asp Ala Met Leu Ala Gln Gly Val Val 
        435                 440                 445 

<210>  2
<211>  1338
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
atgattgaga gcgtcgagtc tttcttggcc cgccttaaaa agcgcgaccc tgaccagccg        60
gagtttcatc aggcagttga ggaagtctta cgctcattat ggccgttcct ggaagctaac       120
ccccattatt tgactagcgg cattcttgaa cgtatttgcg agccggaacg tgccgtcgtt       180
ttccgtgtga gctgggtaga cgaccatgga aaggtgcaag tgaaccgtgg cttccgcatc       240
cagatgaact cagctatcgg cccatataaa ggcgggttgc gttttcatcc aagcgttaat       300
ttgggtgtct taaaattctt agcgttcgag caaacattta aaaacagctt aacatcgtta       360
cccatgggtg gaggaaaggg tggtagtgac ttcgacccaa aggggaagag cgatgcggaa       420
gtcatgcgtt tctgccaggc attcatgtca gagctttacc gtcacatcgg ggcggacgtc       480
gatgtgccag ggggagatat tggcgtgggt gcgcgcgaga ttggattttt attcggtcag       540
tataagcgtc tgtctaacca gttcacctcg gtacttacgg gtaagggacc gtcatatggc       600
ggcagtttga ttaagccaga agctaccgga tttggttgtg tttattttgc cgaagaaatg       660
cttaagcgcc gtggagaaac cgtggaaggc aagcgtgttg ccattagtgg ctctgggaac       720
gtagcgcagt atgcggcccg caaggtgatg gatcttggcg gaaaagtcat ttctttatca       780
gacagcgagg gcacattata ctgcgaatcc ggtttgactg aagctcaatg gcaagcagtg       840
ttggaactga agaatgtaca acgtggccgt atttcagaat tagccgcacg ctttggtctt       900
gaatttcgag cgggccaaca cccctggggt ttatcttgcg atatcgccct tccttgcgcg       960
acgcagaacg agcttgacgc cgaagctgcg cgtgctttac ttcgtaatgg atgcatctgc      1020
gtcgctgaag gggcgaacat gccgacaacc cttgaggcgg ttgatctgtt tatcgaagcg      1080
ggtattctgt tcgctccagg taaagcctcg aatgctggcg gggttgcagt gtcgggttta      1140
gagatgtcgc aaaacgcaat gcgtttattg tggacagggg gcgaggttga ctcaaaattg      1200
catgctatca tgcagagcat ccatcatgct tgcgtacatt acggtgaaga gaacggtcag      1260
gtaaactacg taaagggggc gaatattgct ggattcgtga aggttgctga tgcaatgctg      1320
gcacaggggg tcgtctaa                                                    1338

<210>  3
<211>  1197
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
atgaccaaag ttctggccgt gctgtatccg gatccggtgg atggttttcc gccgaaatat        60
gttcgtgatg atattccgaa aatcacccat tatccggatg gcagtaccgt tccgaccccg       120
gaaggcattg attttaaacc gggtgaactg ctgggtagcg ttagtggcgg tctgggcctg       180
aaaaaatatc tggaaagtaa aggtgtggaa tttgttgtta ccagtgataa agaaggcccg       240
gatagtgtgt ttgaaaaaga actgccgacc gccgatgtgg ttattagtca gccgttttgg       300
ccggcctatc tgaccgcaga tctgattgat aaagcaaaaa agctgaaact ggcaattacc       360
gccggtattg gcagcgatca tgtggatctg aatgccgcca atgaacataa tattaccgtt       420
gcagaagtga cctatagcaa tagtgttagt gttgcagaag cagaagtgat gcagctgctg       480
gccctggtgc gtaattttat tccggcacat gatattgtga aagccggtgg ctggaatatt       540
gcagatgcag ttagccgtgc ctatgatctg gaaggtatga ccgttggtgt gattggtgca       600
ggccgcattg gtcgtgccgt tctggaacgt ctgaaaccgt ttggcgttaa actggtgtat       660
aatcagcgcc aacagctgcc ggatgaagtt gaaaatgaac tgggcctgac ctattttccg       720
gatgttcatg aaatggtgaa agttgtggat gccgttgttc tggcagcacc gctgcatgca       780
cagacctatc atctgtttaa tgatgaagtt ctggccacca tgaaacgtgg cgcctatatt       840
gtgaataata gccgcggcga agaagttgat cgcgatgcaa ttgttcgcgc actgaatagc       900
ggtcagattg gcggttatag tggcgatgtt tggtatccgc agccggcacc gaaagatcat       960
ccgtggcgta ccatgccgaa tgaagcaatg accccgcata tgagtggcac caccctgagt      1020
gcccaggcac gctatgccgc aggtgcacgt gaaattctgg aagattttct ggaagataaa      1080
ccgattcgtc cggaatatct gattgcccag ggtggtagtc tggccggtac cggtgccaaa      1140
agttataccg tgaaaaaagg cgaagaaacc ccgggtagcg gcgaagcaga aaaataa         1197

<210>  4
<211>  27
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
ggcagtttga ttnnkccaga agctacc                                            27

<210>  5
<211>  27
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
ggtagcttct ggmnnaatca aactgcc                                            27

<210>  6
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
gtcgatgtgc cannkggaga tattggcg                                           28

<210>  7
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  7
acgccaatat ctccmnntgg cacatcga                                           28

<210>  8
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
tataattcat atacatccat gggttataat tc                                      32

<210>  9
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
gaattataac ccatggatgt atatgaatta ta                                      32

<210>  10
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
tataattcat atacatatgg gttataattc                                         30

<210>  11
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
gaattataac ccatatgtat atgaattata                                         30

<210>  12
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
tataattcat atacatgggt tataattc                                           28

<210>  13
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  13
gaattataac ccatgtatat gaattata                                           28

<210>  14
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
tataattcat atatgggtta taattc                                             26

<210>  15
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  15
gaattataac ccatatatga attata                                             26

<210>  16
<211>  14
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  16
atatacatcc agat                                                          14

<210>  17
<211>  13
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  17
atatacatcc aga                                                           13

<210>  18
<211>  10
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  18
atatacatcc                                                               10

<210>  19
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  19
gaagtgacct atagcaatnn kgttagtgtt gc                                      32

<210>  20
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  20
ctgcttctgc aacactaacm nnattgctat ag                                      32

<210>  21
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  21
cgttaaactg gtgtataatn nkcgccatca gc                                      32

<210>  22
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  22
ccggcagctg atggcgmnna ttatacacc                                          29

<210>  23
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  23
ctggtgtata atcagnnkca tcagctgccg                                         30

<210>  24
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  24
cttcatccgg cagctgatgm nnctgattat ac                                      32

<210>  25
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  25
ggtgtataat cagcgcnnkc agctgccgg                                          29

<210>  26
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  26
caacttcatc cggcagctgm nngcgctgat ta                                      32

<210>  27
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  27
gaagcaatga ccccgcatnn kagtggcacc                                         30

<210>  28
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  28
ctcagggtgg tgccactmnn atgcggggtc                                         30

<210>  29
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  29
cccgcatatg agtggcaccn nkctgagtgc c                                       31

<210>  30
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  30
gcgtgcctgg gcactcagmn nggtgccact c                                       31

<210>  31
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  31
ggccggtacc ggtgccnnka gttataccg                                          29

<210>  32
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  32
ccttttttca cggtataact mnnggcaccg g                                       31

<210>  33
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  33
cggtgccaaa agttatnnkg tgaaaaaagg                                         30

<210>  34
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  34
ggtttcttcg ccttttttca cmnnataact tt                                      32

