                         SEQUENCE LISTING

<110>  CSL BEHRING L.L.C.
       CHEN, Chao-Guang
       AZAR, Walid Jhan
       HSU, Arthur Lian-Chi
       ALMA, Christopher Walter
 
<120>  DONOR T-CELLS WITH KILL SWITCH

<130>  Calimmune-097WO

<150>  US 63/044,697
<151>  2020-06-26

<160>  61    

<170>  PatentIn version 3.5

<210>  1
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shHPRT 734

<400>  1
aggatatgcc cttgactatt tgtccgacat agtcaagggc atatcctttt tt               52


<210>  2
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Modified sh734

<400>  2
aggatatgcc cttgactatg ccctgaccca gcatagtcaa gggcatatcc t                51


<210>  3
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shRNA targeting an HPRT gene

<400>  3
aggatatgcc cttgactatt tgtccgacat agtcaagggc atatcc                      46


<210>  4
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shRNA targeting an HPRT gene

<400>  4
tcctatacgg gaactgataa acaggctgta tcagttcccg tatagg                      46


<210>  5
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shHPRT 616

<400>  5
gcaggcagta taatccaaat acctgaccca tatttggatt atactgcctg cttttt           56


<210>  6
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shHPRT 211

<400>  6
ggttatgacc ttgatttata cctgacccat attaaatcaa ggtcataacc ttttt            55


<210>  7
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: shHPRT 734.1

<400>  7
gggatatgcc cttgactaat acctgaccca tattagtcaa gggcatatcc cttttt           56


<210>  8
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA734 de novo (DNA form)

<400>  8
acccgtacat atttttgtgt agctctagtt tatagtcaag ggcatatcct tgtgtttttt       60

ttgaaggata tgcccttgac tataaactag cgctacactt tttcgtcttg t               111


<210>  9
<211>  111
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA211 de novo (DNA form)

<400>  9
acccgtacat atttttgtgt agctctagtt ataaatcaag gtcataacct tgtgtttttt       60

ttgaaggtta tgaccttgat ttataactag cgctacactt tttcgtcttg t               111


<210>  10
<211>  166
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA211-3G

<400>  10
ccggatcaac gccctaggtt tatgtttgga tgaactgaca tacgcgtatc cgtcttttaa       60

atcaaggtca taaccgtagt gaaatatata ttaaacaggt tatgaccttg atttaaaata      120

cggtaacgcg gaattcgcaa ctattttatc aattttttgc gtcgac                     166


<210>  11
<211>  166
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA734-3G

<400>  11
ccggatcaac gccctaggtt tatgtttgga tgaactgaca tacgcgtatc cgtcttatag       60

tcaagggcat atcctgtagt gaaatatata ttaaacaagg atatgccctt gactataata      120

cggtaacgcg gaattcgcaa ctattttatc aattttttgc gtcgac                     166


<210>  12
<211>  1435
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Homo sapiens hypoxanthine phosphoribosyltransferase 1 (HPRT1), 
       mRNA

<400>  12
ggcggggcct gcttctcctc agcttcaggc ggctgcgacg agccctcagg cgaacctctc       60

ggctttcccg cgcggcgccg cctcttgctg cgcctccgcc tcctcctctg ctccgccacc      120

ggcttcctcc tcctgagcag tcagcccgcg cgccggccgg ctccgttatg gcgacccgca      180

gccctggcgt cgtgattagt gatgatgaac caggttatga ccttgattta ttttgcatac      240

ctaatcatta tgctgaggat ttggaaaggg tgtttattcc tcatggacta attatggaca      300

ggactgaacg tcttgctcga gatgtgatga aggagatggg aggccatcac attgtagccc      360

tctgtgtgct caaggggggc tataaattct ttgctgacct gctggattac atcaaagcac      420

tgaatagaaa tagtgataga tccattccta tgactgtaga ttttatcaga ctgaagagct      480

attgtaatga ccagtcaaca ggggacataa aagtaattgg tggagatgat ctctcaactt      540

taactggaaa gaatgtcttg attgtggaag atataattga cactggcaaa acaatgcaga      600

ctttgctttc cttggtcagg cagtataatc caaagatggt caaggtcgca agcttgctgg      660

tgaaaaggac cccacgaagt gttggatata agccagactt tgttggattt gaaattccag      720

acaagtttgt tgtaggatat gcccttgact ataatgaata cttcagggat ttgaatcatg      780

tttgtgtcat tagtgaaact ggaaaagcaa aatacaaagc ctaagatgag agttcaagtt      840

gagtttggaa acatctggag tcctattgac atcgccagta aaattatcaa tgttctagtt      900

ctgtggccat ctgcttagta gagctttttg catgtatctt ctaagaattt tatctgtttt      960

gtactttaga aatgtcagtt gctgcattcc taaactgttt atttgcacta tgagcctata     1020

gactatcagt tccctttggg cggattgttg tttaacttgt aaatgaaaaa attctcttaa     1080

accacagcac tattgagtga aacattgaac tcatatctgt aagaaataaa gagaagatat     1140

attagttttt taattggtat tttaattttt atatatgcag gaaagaatag aagtgattga     1200

atattgttaa ttataccacc gtgtgttaga aaagtaagaa gcagtcaatt ttcacatcaa     1260

agacagcatc taagaagttt tgttctgtcc tggaattatt ttagtagtgt ttcagtaatg     1320

ttgactgtat tttccaactt gttcaaatta ttaccagtga atctttgtca gcagttccct     1380

tttaaatgca aatcaataaa ttcccaaaaa tttaaaaaaa aaaaaaaaaa aaaaa          1435


<210>  13
<211>  299
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: 7sk/sh734 expression cassette sequence

<400>  13
accatcgacg tgcagtattt agcatgcccc acccatctgc aaggcattct ggatagtgtc       60

aaaacagccg gaaatcaagt ccgtttatct caaactttag cattttggga ataaatgata      120

tttgctatgc tggttaaatt agattttagt taaatttcct gctgaagctc tagtacgata      180

agtaacttga cctaagtgta aagttgagat ttccttcagg tttatatagc ttgtgcgccg      240

cctgggtacc tcaggatatg cccttgacta tttgtccgac atagtcaagg gcatatcct       299


<210>  14
<211>  248
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Homo sapiens cell-line HEK-293 7SK RNA promoter region

<400>  14
tcgacgtgca gtatttagca tgccccaccc atctgcaagg cattctggat agtgtcaaaa       60

cagccggaaa tcaagtccgt ttatctcaaa ctttagcatt ttgggaataa atgatatttg      120

ctatgctggt taaattagat tttagttaaa tttcctgctg aagctctagt acgataagta      180

acttgaccta agtgtaaagt tgagatttcc ttcaggttta tatagcttgt gcgccgcctg      240

ggtacctc                                                               248


<210>  15
<211>  248
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Homo sapiens cell-line HEK-293 7SK RNA promoter region with 
       mutation 1

<400>  15
tcgacgtgca gtcgggctac tgccccaccc atagtaccgg cattctggat agtgtcaaaa       60

cagccggaaa tcaagtccgt ttatctcaaa ctttagcatt ttgggaataa atgatatttg      120

ctatgctggt taaattagat tttagttaaa tttcctgctg aagctctagt acgataagta      180

acttgaccta agtgtaaagt tgagatttcc ttcaggttta tatagcttgt gcgccgcctg      240

ggtacctc                                                               248


<210>  16
<211>  3901
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: TL20 viral Backbone

<400>  16
ggccgcctcg gccaaacagc ccttgagttt accactccct atcagtgata gagaaaagtg       60

aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact      120

ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga      180

aaagtgaaag tcgagtttac cagtccctat cagtgataga gaaaagtgaa agtcgagttt      240

accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga      300

tagagaaaag tgaaagtcga gctcgccatg ggaggcgtgg cctgggcggg actggggagt      360

ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg      420

ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct      480

caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt      540

aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga      600

acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg      660

ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga      720

ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa      780

ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa      840

aacatatagt atgggcaagc agggagctag aacgattcgc agttaatact ggcctgttag      900

aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat      960

cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga     1020

tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta     1080

agaaaaaagc acagcaagca gcaggatctt cagacctgga aattccctac aatccccaaa     1140

gtcaaggagt agtagaatct atgaataaag aattaaagaa aattatagga caggtaagag     1200

atcaggctga acatcttaag acagcagtac aaatggcagt attcatccac aattttaaaa     1260

gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag     1320

acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt     1380

acagggacag cagaaatcca ctttggaaag gaccagcaaa gctcctctgg aaaggtgaag     1440

gggcagtagt aatacaagat aatagtgaca taaaagtagt gccaagaaga aaagcaaaga     1500

tcattaggga ttatggaaaa cagatggcag gtgatgattg tgtggcaagt agacaggatg     1560

aggattagaa catggaaaag tttagtaaaa caccataagg aggagatatg agggacaatt     1620

ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca     1680

ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt     1740

tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg     1800

tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta     1860

ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa     1920

gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct     1980

ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc     2040

tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca     2100

caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag     2160

aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc     2220

tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt     2280

ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga     2340

cccacctccc aaccccgagg ggaccgagct caagcttcga acgcgtgcgg ccgcatcgat     2400

gccgtagtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta     2460

aaagaaaagg ggggactgga agggctaatt cactcccaaa gaagacaaga tccctgcagg     2520

cattcaaggc caggctggat gtggctctgg gcagcctggg ctgctggttg atgaccctgc     2580

acatagcagg gggttggatc tggatgagca ctgtgctcct ttgcaaccca ggccgttcta     2640

tgattctgtc attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg     2700

tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt     2760

ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac     2820

cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat     2880

ccctgggggc tttggggggg ggctgtcccc gtgagctccc cagatctgct ttttgcctgt     2940

actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac     3000

ccactgctta agcctcaata aagcttcagc tgctcgagct agcagatctt tttccctctg     3060

ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga     3120

aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat     3180

atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat     3240

atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga     3300

aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt     3360

tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg     3420

ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc     3480

tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt     3540

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag     3600

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg     3660

cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa     3720

ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt     3780

ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct     3840

ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc     3900

t                                                                     3901


<210>  17
<211>  6565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: pTL20c Plasmid Sequence

<400>  17
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cggccgcctc ggccaaacag      420

cccttgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc      480

cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa      540

aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta      600

ccagtcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat      660

agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg      720

agctcgccat gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg      780

catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct      840

gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag      900

tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac      960

ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa     1020

agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag     1080

aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga     1140

gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa     1200

aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag     1260

cagggagcta gaacgattcg cagttaatac tggcctgtta gaaacatcag aaggctgtag     1320

acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt     1380

atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa     1440

ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaaaaaag cacagcaagc     1500

agcaggatct tcagacctgg aaattcccta caatccccaa agtcaaggag tagtagaatc     1560

tatgaataaa gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa     1620

gacagcagta caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg     1680

gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt     1740

acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagaaatcc     1800

actttggaaa ggaccagcaa agctcctctg gaaaggtgaa ggggcagtag taatacaaga     1860

taatagtgac ataaaagtag tgccaagaag aaaagcaaag atcattaggg attatggaaa     1920

acagatggca ggtgatgatt gtgtggcaag tagacaggat gaggattaga acatggaaaa     1980

gtttagtaaa acaccataag gaggagatat gagggacaat tggagaagtg aattatataa     2040

atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt     2100

ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc     2160

agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt     2220

gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct     2280

gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag     2340

atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac     2400

cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca     2460

cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt     2520

aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa     2580

atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt     2640

cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt     2700

gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag     2760

gggaccgagc tcaagcttcg aacgcgtgcg gccgcatcga tgccgtagta cctttaagac     2820

caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg     2880

aagggctaat tcactcccaa agaagacaag atccctgcag gcattcaagg ccaggctgga     2940

tgtggctctg ggcagcctgg gctgctggtt gatgaccctg cacatagcag ggggttggat     3000

ctggatgagc actgtgctcc tttgcaaccc aggccgttct atgattctgt cattctaaat     3060

ctctctttca gcctaaagct ttttccccgt atccccccag gtgtctgcag gctcaaagag     3120

cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc     3180

ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg     3240

gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg     3300

gggctgtccc cgtgagctcc ccagatctgc tttttgcctg tactgggtct ctctggttag     3360

accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat     3420

aaagcttcag ctgctcgagc tagcagatct ttttccctct gccaaaaatt atggggacat     3480

catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat     3540

agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt     3600

aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc     3660

catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca     3720

ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt     3780

gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt     3840

cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga     3900

cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc     3960

cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct     4020

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa     4080

acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac     4140

tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact     4200

aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta     4260

gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctgtcgactg cagaggcctg     4320

catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc     4380

acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga     4440

gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg     4500

tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg     4560

cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg     4620

gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga     4680

aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg     4740

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag     4800

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     4860

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     4920

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     4980

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     5040

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     5100

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     5160

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     5220

gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc     5280

ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat     5340

cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt     5400

ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt     5460

tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc     5520

agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc     5580

gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata     5640

ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg     5700

gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc     5760

cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct     5820

acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa     5880

cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt     5940

cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca     6000

ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac     6060

tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca     6120

atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt     6180

tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc     6240

actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca     6300

aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata     6360

ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc     6420

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     6480

cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat     6540

aggcgtatca cgaggccctt tcgtc                                           6565


<210>  18
<211>  592
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: WPRE Sequence

<400>  18
aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct       60

ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt      120

atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg      180

tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact      240

ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct      300

attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg      360

ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc      420

gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc      480

aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt      540

cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tg              592


<210>  19
<211>  769
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: REV response element

<400>  19
aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat       60

tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag      120

agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg      180

cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca      240

gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg      300

gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca      360

gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa      420

tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg      480

ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa      540

ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa      600

ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg      660

cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg      720

atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccg                  769


<210>  20
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Hairpin loop sequence of sh734

<400>  20
ttgtccgac                                                                9


<210>  21
<211>  9
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: hsa-miR-22 loop sequence

<400>  21
ccugaccca                                                                9


<210>  22
<211>  111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA734 de novo (RNA form)

<400>  22
acccguacau auuuuugugu agcucuaguu uauagucaag ggcauauccu uguguuuuuu       60

uugaaggaua ugcccuugac uauaaacuag cgcuacacuu uuucgucuug u               111


<210>  23
<211>  111
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA211 de novo (RNA form)

<400>  23
acccguacau auuuuugugu agcucuaguu auaaaucaag gucauaaccu uguguuuuuu       60

uugaagguua ugaccuugau uuauaacuag cgcuacacuu uuucgucuug u               111


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: miRNA 451 hairpin sequence

<400>  24
aaaccgttac cattactgag tttagtaatg gtaatggttc tc                          42


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  25
gttatggcga cccgcagccc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  26
gcgggtcgcc ataacggagc                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  27
cgtgacgtaa agccgaaccc                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  28
ggcacggaaa gcgaccacct                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  29
catgcgtatt tgacacacga                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  30
gctaagtgct agagttacgg                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  31
gaggcgcggg atccgcagtg                                                   20


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sequence with Chromosome X targeted by a gRNA

<400>  32
ttattactgt tccccgccag                                                   20


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: IN-1

<400>  33
attatgctga ggatttggaa                                                   20


<210>  34
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: IDT-1

<400>  34
gatgatctct ctcaacttta ac                                                22


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: IDT-6

<400>  35
catacctaat cattatgctg                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: IDT-4

<400>  36
ggttatgacc ttgatttatt                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: IDT-2

<400>  37
catggactaa ttatggacag                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: IDT-3

<400>  38
tagccctctg tgtgctcaag                                                   20


<210>  39
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Nat Paper

<400>  39
gccctggccg gttcaggccc acg                                               23


<210>  40
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 12

<400>  40
gccccccttg agcacacaga ggg                                               23


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 13

<400>  41
agcccccctt gagcacacag agg                                               23


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 15

<400>  42
gatgtgatga aggagatggg agg                                               23


<210>  43
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 16

<400>  43
ctgataaaat ctacagtcat agg                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 17

<400>  44
gtagccctct gtgtgctcaa ggg                                               23


<210>  45
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 18

<400>  45
ttatgctgag gatttggaaa ggg                                               23


<210>  46
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 19

<400>  46
gtgctttgat gtaatccagc agg                                               23


<210>  47
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 20

<400>  47
tgaagtattc attatagtca agg                                               23


<210>  48
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 21

<400>  48
tatcctacaa caaacttgtc tgg                                               23


<210>  49
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: gRNA 22

<400>  49
gaagtattca ttatagtcaa ggg                                               23


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  50
tagcccccct tgagcacaca                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  51
atgtaatcca gcaggtcagc                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  52
tattcagtgc tttgatgtaa                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  53
ctgacctgct ggattacatc                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  54
tcagactgaa gagctattgt                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  55
aaattccaga caagtttgtt                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  56
ttgtaggata tgcccttgac                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  57
caaatcctca gcataatgat                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  58
ttttgcatac ctaatcatta                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  59
catacctaat cattatgctg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  60
gaaagggtgt ttattcctca                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  61
ttcctcatgg actaattatg                                                   20


