                         SEQUENCE LISTING

<110>  Cilian AG
 
<120>  New lipase enzyme

<130>  CD 42798

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  272
<212>  PRT
<213>  Tetrahymena thermophila

<400>  1

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Gln Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  2
<211>  867
<212>  DNA
<213>  Tetrahymena thermophila

<400>  2
atgaaattgt aattgcttct attggtttgc ttgtcatttg ctgcctgcta atcatttact       60

tatacttaat cacttgctta agacttagct ggtttctctc ttgcttctta ctgtaatcct      120

aaatctatag aacaatggaa ttgtggatgt gcttgtgata aaaaccctta aggacttcga      180

aatgttacta tcttatttaa ctctactcta taagctagtg gatatttagg ctactccact      240

catcatgatg caattgttgt tgtattcaga ggaacagtac cttggttaat cgaaaattgg      300

attgctgact taaacacctt caagacttag tacccactct gccaaaactg ttatgtccat      360

taaggctttt ataaccagtt caaataattg aaatctcagc ttgttactag ctttacttca      420

cttcgttaac tatatcctaa tgcaaaagta tttgttacag gacattctct tggtgctgca      480

atgagtgctc actcaatacc agtaatttac taattaaatg gaaataaacc tattgatgct      540

ttttacaatt atggttgtcc tagagtaggt gactaaactt atgcaaactg gtttaacagt      600

taaaattttg ccttagaata tggtagaatt aataatgctg ctgatccagt tcctcattta      660

cctcctcttc tttacccatt ttcatttttc cactacaacc atgaaatatt ctatccttct      720

tttgttcttt ttggaaacta acataactaa tgttaaaacg cggaaacaat atttggtgca      780

gatggagtaa taatagcagc taatgttcta gaccatctaa cttattttgg atgggattgg      840

tctggttcta tattaacttg ctaatga                                          867


<210>  3
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  3

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Lys Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  4
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  4

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Arg Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  5
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  5

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu His Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  6
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  6

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Gln Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Ile Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Ile Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  7
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  7

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Gln Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Ile Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Ile Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  8
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  8

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Gln Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Ile Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Phe Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  9
<211>  16
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lead peptide

<400>  9

Met Lys Leu Gln Leu Leu Leu Leu Val Cys Leu Ser Phe Ala Ala Cys 
1               5                   10                  15      


<210>  10
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  10

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Lys Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Ile Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Ile Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  11
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  11

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Lys Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Ile Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Ile Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  12
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  12

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Lys Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Ile Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Phe Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  13
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  13

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Arg Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Ile Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Ile Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  14
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  14

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Arg Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Ile Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Ile Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  15
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  15

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu Arg Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Ile Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Phe Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  16
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  16

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu His Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Ile Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Ile Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  17
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  17

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu His Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Ile Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Val Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Tyr Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Ile Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


<210>  18
<211>  272
<212>  PRT
<213>  artificial sequence

<220>
<223>  Lipase mutant

<400>  18

Gln Ser Phe Thr Tyr Thr Gln Ser Leu Ala Gln Asp Leu Ala Gly Phe 
1               5                   10                  15      


Ser Leu Ala Ser Tyr Cys Asn Pro Lys Ser Ile Glu Gln Trp Asn Cys 
            20                  25                  30          


Gly Cys Ala Cys Asp Lys Asn Pro Gln Gly Leu Arg Asn Val Thr Ile 
        35                  40                  45              


Leu Phe Asn Ser Thr Leu His Ala Ser Gly Tyr Leu Gly Tyr Ser Thr 
    50                  55                  60                  


His His Asp Ala Ile Val Val Val Phe Arg Gly Thr Val Pro Trp Leu 
65                  70                  75                  80  


Ile Glu Asn Trp Ile Ala Asp Leu Asn Thr Phe Lys Thr Gln Tyr Pro 
                85                  90                  95      


Leu Cys Gln Asn Cys Tyr Val His Gln Gly Phe Tyr Asn Gln Phe Lys 
            100                 105                 110         


Gln Leu Lys Ser Gln Leu Ile Thr Ser Phe Thr Ser Leu Arg Gln Leu 
        115                 120                 125             


Tyr Pro Asn Ala Lys Val Phe Val Thr Gly His Ser Leu Gly Ala Ala 
    130                 135                 140                 


Met Ser Ala His Ser Ile Pro Val Ile Tyr Gln Leu Asn Gly Asn Lys 
145                 150                 155                 160 


Pro Ile Asp Ala Phe Tyr Asn Phe Gly Cys Pro Arg Val Gly Asp Gln 
                165                 170                 175     


Thr Tyr Ala Asn Trp Phe Asn Ser Gln Asn Phe Ala Leu Glu Tyr Gly 
            180                 185                 190         


Arg Ile Asn Asn Ala Ala Asp Pro Val Pro His Leu Pro Pro Leu Leu 
        195                 200                 205             


Tyr Pro Phe Ser Phe Phe His Tyr Asn His Glu Ile Phe Tyr Pro Ser 
    210                 215                 220                 


Phe Val Leu Phe Gly Asn Gln His Asn Gln Cys Gln Asn Ala Glu Thr 
225                 230                 235                 240 


Ile Phe Gly Ala Asp Gly Val Ile Ile Ala Ala Asn Val Leu Asp His 
                245                 250                 255     


Leu Thr Tyr Phe Gly Trp Asp Trp Ser Gly Ser Ile Leu Thr Cys Gln 
            260                 265                 270         


