                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals SA
 
<120>  MODIFIED PROTEINS

<130>  VB66928 FF

<150>  EP20182138.6
<151>  2020-06-25

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  612
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  1

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly 
        275                 280                 285             


Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser 
    290                 295                 300                 


Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly 
305                 310                 315                 320 


Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala 
                325                 330                 335     


Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg 
            340                 345                 350         


Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val 
        355                 360                 365             


Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp 
    370                 375                 380                 


Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe 
385                 390                 395                 400 


Leu Gly Asp Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn 
                405                 410                 415     


Trp Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg 
            420                 425                 430         


Gly Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln 
        435                 440                 445             


Ser Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala 
    450                 455                 460                 


Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly 
465                 470                 475                 480 


Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly 
                485                 490                 495     


Ala Leu Leu Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr 
            500                 505                 510         


Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu 
        515                 520                 525             


Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly 
    530                 535                 540                 


Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala 
545                 550                 555                 560 


Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn 
                565                 570                 575     


Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala 
            580                 585                 590         


Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg 
        595                 600                 605             


Glu Asp Leu Lys 
    610         


<210>  2
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be Ser or Thr

<400>  2

Xaa Xaa Asn Xaa Xaa 
1               5   


<210>  3
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<400>  3

Lys Xaa Xaa Asn Xaa Xaa Lys 
1               5           


<210>  4
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Consensus sequence

<400>  4

Lys Asp Gln Asn Ala Thr Lys 
1               5           


<210>  5
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  Consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be Ser or Thr

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

Xaa Xaa Xaa Asn Xaa Xaa Xaa 
1               5           


<210>  6
<211>  624
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       Y208+R274

<400>  6

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys 
        195                 200                 205             


Asp Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp 
    210                 215                 220                 


Thr Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys 
225                 230                 235                 240 


His Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe 
                245                 250                 255     


Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
            260                 265                 270         


Leu Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn Ala Thr Lys His Arg 
        275                 280                 285             


Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln 
    290                 295                 300                 


Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val 
305                 310                 315                 320 


Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp 
                325                 330                 335     


Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu 
            340                 345                 350         


Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly 
        355                 360                 365             


Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys 
    370                 375                 380                 


Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
385                 390                 395                 400 


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
                405                 410                 415     


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
            420                 425                 430         


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
        435                 440                 445             


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
    450                 455                 460                 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
465                 470                 475                 480 


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
                485                 490                 495     


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
            500                 505                 510         


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
        515                 520                 525             


Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly 
    530                 535                 540                 


His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu 
545                 550                 555                 560 


Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val 
                565                 570                 575     


Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp 
            580                 585                 590         


Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu 
        595                 600                 605             


Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615                 620                 


<210>  7
<211>  630
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       Y208+R274+A519

<400>  7

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys 
        195                 200                 205             


Asp Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp 
    210                 215                 220                 


Thr Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys 
225                 230                 235                 240 


His Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe 
                245                 250                 255     


Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
            260                 265                 270         


Leu Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn Ala Thr Lys His Arg 
        275                 280                 285             


Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln 
    290                 295                 300                 


Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val 
305                 310                 315                 320 


Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp 
                325                 330                 335     


Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu 
            340                 345                 350         


Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly 
        355                 360                 365             


Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys 
    370                 375                 380                 


Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
385                 390                 395                 400 


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
                405                 410                 415     


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
            420                 425                 430         


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
        435                 440                 445             


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
    450                 455                 460                 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
465                 470                 475                 480 


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
                485                 490                 495     


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
            500                 505                 510         


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
        515                 520                 525             


Thr Leu Lys Asp Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu 
    530                 535                 540                 


Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile 
545                 550                 555                 560 


Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro 
                565                 570                 575     


Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro 
            580                 585                 590         


Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu 
        595                 600                 605             


Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro 
    610                 615                 620                 


Pro Arg Glu Asp Leu Lys 
625                 630 


<210>  8
<211>  647
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+Y208+R274+A519

<400>  8

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Lys 
1               5                   10                  15      


Leu Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys 
            20                  25                  30          


Val Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp 
        35                  40                  45              


Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met 
    50                  55                  60                  


Val Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala 
65                  70                  75                  80  


Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val 
                85                  90                  95      


Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly 
            100                 105                 110         


Ser Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser 
        115                 120                 125             


Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser 
    130                 135                 140                 


His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala 
145                 150                 155                 160 


Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn 
                165                 170                 175     


Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val 
            180                 185                 190         


Met Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala 
        195                 200                 205             


Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn 
    210                 215                 220                 


Lys Asp Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp 
225                 230                 235                 240 


Asp Thr Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala 
                245                 250                 255     


Lys His Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His 
            260                 265                 270         


Phe Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys 
        275                 280                 285             


His Leu Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn Ala Thr Lys His 
    290                 295                 300                 


Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val 
305                 310                 315                 320 


Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln 
                325                 330                 335     


Val Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly 
            340                 345                 350         


Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala 
        355                 360                 365             


Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr 
    370                 375                 380                 


Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr 
385                 390                 395                 400 


Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp 
                405                 410                 415     


Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp 
            420                 425                 430         


Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val 
        435                 440                 445             


Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val 
    450                 455                 460                 


Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val 
465                 470                 475                 480 


Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg 
                485                 490                 495     


Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln 
            500                 505                 510         


Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu 
        515                 520                 525             


Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly 
    530                 535                 540                 


Leu Thr Leu Lys Asp Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly 
545                 550                 555                 560 


Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala 
                565                 570                 575     


Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp 
            580                 585                 590         


Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp 
        595                 600                 605             


Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys 
    610                 615                 620                 


Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys 
625                 630                 635                 640 


Pro Pro Arg Glu Asp Leu Lys 
                645         


<210>  9
<211>  658
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+Y208+R274+A519+C-terminal

<400>  9

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Ala 
1               5                   10                  15      


Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val Leu 
            20                  25                  30          


Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro Ala 
        35                  40                  45              


Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val Leu 
    50                  55                  60                  


Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu Ser 
65                  70                  75                  80  


Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu Pro 
                85                  90                  95      


Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser Trp 
            100                 105                 110         


Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn Ile 
        115                 120                 125             


Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His Met 
    130                 135                 140                 


Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys Leu 
145                 150                 155                 160 


Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu Met 
                165                 170                 175     


Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met Ala 
            180                 185                 190         


Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser Gly 
        195                 200                 205             


Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys Asp 
    210                 215                 220                 


Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr 
225                 230                 235                 240 


Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His 
                245                 250                 255     


Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe Pro 
            260                 265                 270         


Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His Leu 
        275                 280                 285             


Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn Ala Thr Lys His Arg Gln 
    290                 295                 300                 


Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg 
305                 310                 315                 320 


Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp 
                325                 330                 335     


Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu 
            340                 345                 350         


Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr 
        355                 360                 365             


Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn 
    370                 375                 380                 


Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro 
385                 390                 395                 400 


Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu 
                405                 410                 415     


Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly 
            420                 425                 430         


Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg 
        435                 440                 445             


Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val 
    450                 455                 460                 


Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly 
465                 470                 475                 480 


Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe 
                485                 490                 495     


Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln 
            500                 505                 510         


Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val 
        515                 520                 525             


Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr 
    530                 535                 540                 


Leu Lys Asp Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu Val 
545                 550                 555                 560 


Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr 
                565                 570                 575     


Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu 
            580                 585                 590         


Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg 
        595                 600                 605             


Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln 
    610                 615                 620                 


Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro 
625                 630                 635                 640 


Arg Glu Asp Leu Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser 
                645                 650                 655     


Gly Gly 
        


<210>  10
<211>  659
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+Y208+S318+A519+C-terminal

<400>  10

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Ala 
1               5                   10                  15      


Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val Leu 
            20                  25                  30          


Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro Ala 
        35                  40                  45              


Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val Leu 
    50                  55                  60                  


Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu Ser 
65                  70                  75                  80  


Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu Pro 
                85                  90                  95      


Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser Trp 
            100                 105                 110         


Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn Ile 
        115                 120                 125             


Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His Met 
    130                 135                 140                 


Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys Leu 
145                 150                 155                 160 


Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu Met 
                165                 170                 175     


Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met Ala 
            180                 185                 190         


Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser Gly 
        195                 200                 205             


Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys Asp 
    210                 215                 220                 


Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr 
225                 230                 235                 240 


Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His 
                245                 250                 255     


Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe Pro 
            260                 265                 270         


Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His Leu 
        275                 280                 285             


Pro Leu Glu Ala Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln 
    290                 295                 300                 


Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu 
305                 310                 315                 320 


Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala 
                325                 330                 335     


Leu Ala Lys Asp Gln Asn Ala Thr Lys Ser Pro Gly Ser Gly Gly Asp 
            340                 345                 350         


Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu 
        355                 360                 365             


Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly 
    370                 375                 380                 


Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys 
385                 390                 395                 400 


Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
                405                 410                 415     


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
            420                 425                 430         


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
        435                 440                 445             


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
    450                 455                 460                 


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
465                 470                 475                 480 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
                485                 490                 495     


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
            500                 505                 510         


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
        515                 520                 525             


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
    530                 535                 540                 


Thr Leu Lys Asp Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu 
545                 550                 555                 560 


Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile 
                565                 570                 575     


Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro 
            580                 585                 590         


Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro 
        595                 600                 605             


Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu 
    610                 615                 620                 


Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro 
625                 630                 635                 640 


Pro Arg Glu Asp Leu Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly 
                645                 650                 655     


Ser Gly Gly 
            


<210>  11
<211>  659
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+R274+S318+A519+C-terminal

<400>  11

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Ala 
1               5                   10                  15      


Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val Leu 
            20                  25                  30          


Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro Ala 
        35                  40                  45              


Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val Leu 
    50                  55                  60                  


Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu Ser 
65                  70                  75                  80  


Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu Pro 
                85                  90                  95      


Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser Trp 
            100                 105                 110         


Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn Ile 
        115                 120                 125             


Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His Met 
    130                 135                 140                 


Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys Leu 
145                 150                 155                 160 


Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu Met 
                165                 170                 175     


Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met Ala 
            180                 185                 190         


Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser Gly 
        195                 200                 205             


Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr Leu 
    210                 215                 220                 


Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile Tyr 
225                 230                 235                 240 


Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys Pro 
                245                 250                 255     


Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu Ala 
            260                 265                 270         


Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe Thr 
        275                 280                 285             


Lys Asp Gln Asn Ala Thr Lys His Arg Gln Pro Arg Gly Trp Glu Gln 
    290                 295                 300                 


Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu 
305                 310                 315                 320 


Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala 
                325                 330                 335     


Leu Ala Lys Asp Gln Asn Ala Thr Lys Ser Pro Gly Ser Gly Gly Asp 
            340                 345                 350         


Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu 
        355                 360                 365             


Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly 
    370                 375                 380                 


Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys 
385                 390                 395                 400 


Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
                405                 410                 415     


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
            420                 425                 430         


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
        435                 440                 445             


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
    450                 455                 460                 


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
465                 470                 475                 480 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
                485                 490                 495     


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
            500                 505                 510         


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
        515                 520                 525             


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
    530                 535                 540                 


Thr Leu Lys Asp Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu 
545                 550                 555                 560 


Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile 
                565                 570                 575     


Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro 
            580                 585                 590         


Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro 
        595                 600                 605             


Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu 
    610                 615                 620                 


Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro 
625                 630                 635                 640 


Pro Arg Glu Asp Leu Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly 
                645                 650                 655     


Ser Gly Gly 
            


<210>  12
<211>  665
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+Y208+R274+S318+A519+C-terminal

<400>  12

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Ala 
1               5                   10                  15      


Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val Leu 
            20                  25                  30          


Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro Ala 
        35                  40                  45              


Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val Leu 
    50                  55                  60                  


Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu Ser 
65                  70                  75                  80  


Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu Pro 
                85                  90                  95      


Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser Trp 
            100                 105                 110         


Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn Ile 
        115                 120                 125             


Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His Met 
    130                 135                 140                 


Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys Leu 
145                 150                 155                 160 


Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu Met 
                165                 170                 175     


Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met Ala 
            180                 185                 190         


Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser Gly 
        195                 200                 205             


Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys Asp 
    210                 215                 220                 


Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr 
225                 230                 235                 240 


Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His 
                245                 250                 255     


Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe Pro 
            260                 265                 270         


Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His Leu 
        275                 280                 285             


Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn Ala Thr Lys His Arg Gln 
    290                 295                 300                 


Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg 
305                 310                 315                 320 


Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp 
                325                 330                 335     


Gln Val Ile Arg Asn Ala Leu Ala Lys Asp Gln Asn Ala Thr Lys Ser 
            340                 345                 350         


Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu 
        355                 360                 365             


Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe 
    370                 375                 380                 


Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp 
385                 390                 395                 400 


Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro 
                405                 410                 415     


Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala 
            420                 425                 430         


Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr 
        435                 440                 445             


Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu 
    450                 455                 460                 


Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala 
465                 470                 475                 480 


Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu 
                485                 490                 495     


Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala 
            500                 505                 510         


Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg 
        515                 520                 525             


Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly 
    530                 535                 540                 


Phe Tyr Arg Thr Gly Leu Thr Leu Lys Asp Gln Asn Ala Thr Lys Ala 
545                 550                 555                 560 


Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro 
                565                 570                 575     


Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Val 
            580                 585                 590         


Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser 
        595                 600                 605             


Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser 
    610                 615                 620                 


Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala 
625                 630                 635                 640 


Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu Gly Ser Gly Gly Gly 
                645                 650                 655     


Asp Gln Asn Ala Thr Gly Ser Gly Gly 
            660                 665 


<210>  13
<211>  658
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+Y208+K240+R274+S318+A519

<400>  13

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Ala 
1               5                   10                  15      


Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val Leu 
            20                  25                  30          


Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro Ala 
        35                  40                  45              


Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val Leu 
    50                  55                  60                  


Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu Ser 
65                  70                  75                  80  


Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu Pro 
                85                  90                  95      


Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser Trp 
            100                 105                 110         


Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn Ile 
        115                 120                 125             


Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His Met 
    130                 135                 140                 


Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys Leu 
145                 150                 155                 160 


Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu Met 
                165                 170                 175     


Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met Ala 
            180                 185                 190         


Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser Gly 
        195                 200                 205             


Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys Asp 
    210                 215                 220                 


Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr 
225                 230                 235                 240 


Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His 
                245                 250                 255     


Asp Leu Asp Ile Lys Asp Asn Asn Asn Ser Thr Pro Thr Val Ile Ser 
            260                 265                 270         


His Arg Leu His Phe Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala 
        275                 280                 285             


His Gln Ala Cys His Leu Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn 
    290                 295                 300                 


Ala Thr Lys His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys 
305                 310                 315                 320 


Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu 
                325                 330                 335     


Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Lys Asp 
            340                 345                 350         


Gln Asn Ala Thr Lys Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala 
        355                 360                 365             


Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala 
    370                 375                 380                 


Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala 
385                 390                 395                 400 


Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala 
                405                 410                 415     


Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg 
            420                 425                 430         


Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser 
        435                 440                 445             


Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln 
    450                 455                 460                 


Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His 
465                 470                 475                 480 


Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg 
                485                 490                 495     


Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala 
            500                 505                 510         


Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp 
        515                 520                 525             


Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro 
    530                 535                 540                 


Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Lys Asp 
545                 550                 555                 560 


Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu 
                565                 570                 575     


Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu 
            580                 585                 590         


Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg 
        595                 600                 605             


Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly 
    610                 615                 620                 


Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser 
625                 630                 635                 640 


Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp 
                645                 650                 655     


Leu Lys 
        


<210>  14
<211>  671
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+Y208+K240+R274+S318+A519+C-terminal

<400>  14

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Ala 
1               5                   10                  15      


Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val Leu 
            20                  25                  30          


Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro Ala 
        35                  40                  45              


Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val Leu 
    50                  55                  60                  


Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu Ser 
65                  70                  75                  80  


Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu Pro 
                85                  90                  95      


Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser Trp 
            100                 105                 110         


Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn Ile 
        115                 120                 125             


Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His Met 
    130                 135                 140                 


Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys Leu 
145                 150                 155                 160 


Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu Met 
                165                 170                 175     


Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met Ala 
            180                 185                 190         


Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser Gly 
        195                 200                 205             


Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys Asp 
    210                 215                 220                 


Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr 
225                 230                 235                 240 


Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His 
                245                 250                 255     


Asp Leu Asp Ile Lys Asp Asn Asn Asn Ser Thr Pro Thr Val Ile Ser 
            260                 265                 270         


His Arg Leu His Phe Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala 
        275                 280                 285             


His Gln Ala Cys His Leu Pro Leu Glu Ala Phe Thr Lys Asp Gln Asn 
    290                 295                 300                 


Ala Thr Lys His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys 
305                 310                 315                 320 


Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu 
                325                 330                 335     


Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Lys Asp 
            340                 345                 350         


Gln Asn Ala Thr Lys Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala 
        355                 360                 365             


Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala 
    370                 375                 380                 


Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala 
385                 390                 395                 400 


Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala 
                405                 410                 415     


Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg 
            420                 425                 430         


Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser 
        435                 440                 445             


Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln 
    450                 455                 460                 


Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His 
465                 470                 475                 480 


Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg 
                485                 490                 495     


Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala 
            500                 505                 510         


Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp 
        515                 520                 525             


Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro 
    530                 535                 540                 


Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Lys Asp 
545                 550                 555                 560 


Gln Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu 
                565                 570                 575     


Ile Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu 
            580                 585                 590         


Glu Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg 
        595                 600                 605             


Thr Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly 
    610                 615                 620                 


Gly Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser 
625                 630                 635                 640 


Ala Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp 
                645                 650                 655     


Leu Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly 
            660                 665                 670     


<210>  15
<211>  19
<212>  PRT
<213>  E. coli

<400>  15

Met Ile Lys Phe Leu Ser Ala Leu Ile Leu Leu Leu Val Thr Thr Ala 
1               5                   10                  15      


Ala Gln Ala 
            


<210>  16
<211>  21
<212>  PRT
<213>  E. coli

<400>  16

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  17
<211>  26
<212>  PRT
<213>  E. coli

<400>  17

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  18
<211>  22
<212>  PRT
<213>  Erwinia carotovorans

<400>  18

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  19
<211>  23
<212>  PRT
<213>  E. coli

<400>  19

Met Ser Phe Lys Lys Ile Ile Lys Ala Phe Val Ile Met Ala Ala Leu 
1               5                   10                  15      


Val Ser Val Gln Ala His Ala 
            20              


<210>  20
<211>  28
<212>  PRT
<213>  Bacillus subtilis

<400>  20

Met Phe Lys Phe Lys Lys Lys Phe Leu Val Gly Leu Thr Ala Ala Phe 
1               5                   10                  15      


Met Ser Ile Ser Met Phe Ser Ala Thr Ala Ser Ala 
            20                  25              


<210>  21
<211>  19
<212>  PRT
<213>  E. coli

<400>  21

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala 
            


<210>  22
<211>  21
<212>  PRT
<213>  E.coli

<400>  22

Met Lys Gln Ala Leu Arg Val Ala Phe Gly Phe Leu Ile Leu Trp Ala 
1               5                   10                  15      


Ser Val Leu His Ala 
            20      


<210>  23
<211>  26
<212>  PRT
<213>  Streptococcus agalactiae

<400>  23

Met Lys Met Asn Lys Lys Val Leu Leu Thr Ser Thr Met Ala Ala Ser 
1               5                   10                  15      


Leu Leu Ser Val Ala Ser Val Gln Ala Ser 
            20                  25      


<210>  24
<211>  713
<212>  PRT
<213>  Campylobacter jejuni

<400>  24

Met Leu Lys Lys Glu Tyr Leu Lys Asn Pro Tyr Leu Val Leu Phe Ala 
1               5                   10                  15      


Met Ile Ile Leu Ala Tyr Val Phe Ser Val Phe Cys Arg Phe Tyr Trp 
            20                  25                  30          


Val Trp Trp Ala Ser Glu Phe Asn Glu Tyr Phe Phe Asn Asn Gln Leu 
        35                  40                  45              


Met Ile Ile Ser Asn Asp Gly Tyr Ala Phe Ala Glu Gly Ala Arg Asp 
    50                  55                  60                  


Met Ile Ala Gly Phe His Gln Pro Asn Asp Leu Ser Tyr Tyr Gly Ser 
65                  70                  75                  80  


Ser Leu Ser Ala Leu Thr Tyr Trp Leu Tyr Lys Ile Thr Pro Phe Ser 
                85                  90                  95      


Phe Glu Ser Ile Ile Leu Tyr Met Ser Thr Phe Leu Ser Ser Leu Val 
            100                 105                 110         


Val Ile Pro Thr Ile Leu Leu Ala Asn Glu Tyr Lys Arg Pro Leu Met 
        115                 120                 125             


Gly Phe Val Ala Ala Leu Leu Ala Ser Ile Ala Asn Ser Tyr Tyr Asn 
    130                 135                 140                 


Arg Thr Met Ser Gly Tyr Tyr Asp Thr Asp Met Leu Val Ile Val Leu 
145                 150                 155                 160 


Pro Met Phe Ile Leu Phe Phe Met Val Arg Met Ile Leu Lys Lys Asp 
                165                 170                 175     


Phe Phe Ser Leu Ile Ala Leu Pro Leu Phe Ile Gly Ile Tyr Leu Trp 
            180                 185                 190         


Trp Tyr Pro Ser Ser Tyr Thr Leu Asn Val Ala Leu Ile Gly Leu Phe 
        195                 200                 205             


Leu Ile Tyr Thr Leu Ile Phe His Arg Lys Glu Lys Ile Phe Tyr Ile 
    210                 215                 220                 


Ala Val Ile Leu Ser Ser Leu Thr Leu Ser Asn Ile Ala Trp Phe Tyr 
225                 230                 235                 240 


Gln Ser Ala Ile Ile Val Ile Leu Phe Ala Leu Phe Ala Leu Glu Gln 
                245                 250                 255     


Lys Arg Leu Asn Phe Met Ile Ile Gly Ile Leu Gly Ser Ala Thr Leu 
            260                 265                 270         


Ile Phe Leu Ile Leu Ser Gly Gly Val Asp Pro Ile Leu Tyr Gln Leu 
        275                 280                 285             


Lys Phe Tyr Ile Phe Arg Ser Asp Glu Ser Ala Asn Leu Thr Gln Gly 
    290                 295                 300                 


Phe Met Tyr Phe Asn Val Asn Gln Thr Ile Gln Glu Val Glu Asn Val 
305                 310                 315                 320 


Asp Leu Ser Glu Phe Met Arg Arg Ile Ser Gly Ser Glu Ile Val Phe 
                325                 330                 335     


Leu Phe Ser Leu Phe Gly Phe Val Trp Leu Leu Arg Lys His Lys Ser 
            340                 345                 350         


Met Ile Met Ala Leu Pro Ile Leu Val Leu Gly Phe Leu Ala Leu Lys 
        355                 360                 365             


Gly Gly Leu Arg Phe Thr Ile Tyr Ser Val Pro Val Met Ala Leu Gly 
    370                 375                 380                 


Phe Gly Phe Leu Leu Ser Glu Phe Lys Ala Ile Met Val Lys Lys Tyr 
385                 390                 395                 400 


Ser Gln Leu Thr Ser Asn Val Cys Ile Val Phe Ala Thr Ile Leu Thr 
                405                 410                 415     


Leu Ala Pro Val Phe Ile His Ile Tyr Asn Tyr Lys Ala Pro Thr Val 
            420                 425                 430         


Phe Ser Gln Asn Glu Ala Ser Leu Leu Asn Gln Leu Lys Asn Ile Ala 
        435                 440                 445             


Asn Arg Glu Asp Tyr Val Val Thr Trp Trp Asp Tyr Gly Tyr Pro Val 
    450                 455                 460                 


Arg Tyr Tyr Ser Asp Val Lys Thr Leu Val Asp Gly Gly Lys His Leu 
465                 470                 475                 480 


Gly Lys Asp Asn Phe Phe Pro Ser Phe Ala Leu Ser Lys Asp Glu Gln 
                485                 490                 495     


Ala Ala Ala Asn Met Ala Arg Leu Ser Val Glu Tyr Thr Glu Lys Ser 
            500                 505                 510         


Phe Tyr Ala Pro Gln Asn Asp Ile Leu Lys Thr Asp Ile Leu Gln Ala 
        515                 520                 525             


Met Met Lys Asp Tyr Asn Gln Ser Asn Val Asp Leu Phe Leu Ala Ser 
    530                 535                 540                 


Leu Ser Lys Pro Asp Phe Lys Ile Asp Thr Pro Lys Thr Arg Asp Ile 
545                 550                 555                 560 


Tyr Leu Tyr Met Pro Ala Arg Met Ser Leu Ile Phe Ser Thr Val Ala 
                565                 570                 575     


Ser Phe Ser Phe Ile Asn Leu Asp Thr Gly Val Leu Asp Lys Pro Phe 
            580                 585                 590         


Thr Phe Ser Thr Ala Tyr Pro Leu Asp Val Lys Asn Gly Glu Ile Tyr 
        595                 600                 605             


Leu Ser Asn Gly Val Val Leu Ser Asp Asp Phe Arg Ser Phe Lys Ile 
    610                 615                 620                 


Gly Asp Asn Val Val Ser Val Asn Ser Ile Val Glu Ile Asn Ser Ile 
625                 630                 635                 640 


Lys Gln Gly Glu Tyr Lys Ile Thr Pro Ile Asp Asp Lys Ala Gln Phe 
                645                 650                 655     


Tyr Ile Phe Tyr Leu Lys Asp Ser Ala Ile Pro Tyr Ala Gln Phe Ile 
            660                 665                 670         


Leu Met Asp Lys Thr Met Phe Asn Ser Ala Tyr Val Gln Met Phe Phe 
        675                 680                 685             


Leu Gly Asn Tyr Asp Lys Asn Leu Phe Asp Leu Val Ile Asn Ser Arg 
    690                 695                 700                 


Asp Ala Lys Val Phe Lys Leu Lys Ile 
705                 710             


<210>  25
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  Consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa can be Asp or Glu

<220>
<221>  MISC_FEATURE
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa can be any naturally occurring amino acid except proline

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Xaa can be Ser or Thr

<400>  25

Gly Ser Gly Gly Gly Xaa Xaa Asn Xaa Xaa Gly Ser Gly Gly 
1               5                   10                  


<210>  26
<211>  624
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       K240+A376

<400>  26

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Asp Asn Asn Asn Ser Thr Pro Thr Val Ile Ser His Arg Leu His Phe 
                245                 250                 255     


Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
            260                 265                 270         


Leu Pro Leu Glu Ala Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu 
        275                 280                 285             


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
    290                 295                 300                 


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
305                 310                 315                 320 


Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
                325                 330                 335     


Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
            340                 345                 350         


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
        355                 360                 365             


Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Lys Asp Gln 
    370                 375                 380                 


Asn Arg Thr Lys Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
385                 390                 395                 400 


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
                405                 410                 415     


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
            420                 425                 430         


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
        435                 440                 445             


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
    450                 455                 460                 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
465                 470                 475                 480 


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
                485                 490                 495     


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
            500                 505                 510         


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
        515                 520                 525             


Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly 
    530                 535                 540                 


His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu 
545                 550                 555                 560 


Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val 
                565                 570                 575     


Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp 
            580                 585                 590         


Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu 
        595                 600                 605             


Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615                 620                 


<210>  27
<211>  652
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+K240+A376+C-terminal

<400>  27

Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Lys 
1               5                   10                  15      


Leu Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys 
            20                  25                  30          


Val Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp 
        35                  40                  45              


Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met 
    50                  55                  60                  


Val Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala 
65                  70                  75                  80  


Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val 
                85                  90                  95      


Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly 
            100                 105                 110         


Ser Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser 
        115                 120                 125             


Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser 
    130                 135                 140                 


His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala 
145                 150                 155                 160 


Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn 
                165                 170                 175     


Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val 
            180                 185                 190         


Met Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala 
        195                 200                 205             


Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn 
    210                 215                 220                 


Tyr Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys 
225                 230                 235                 240 


Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile 
                245                 250                 255     


Lys Asp Asn Asn Asn Ser Thr Pro Thr Val Ile Ser His Arg Leu His 
            260                 265                 270         


Phe Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys 
        275                 280                 285             


His Leu Pro Leu Glu Ala Phe Thr Arg His Arg Gln Pro Arg Gly Trp 
    290                 295                 300                 


Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu 
305                 310                 315                 320 


Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg 
                325                 330                 335     


Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile 
            340                 345                 350         


Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala 
        355                 360                 365             


Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly 
    370                 375                 380                 


Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Lys Asp 
385                 390                 395                 400 


Gln Asn Arg Thr Lys Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp 
                405                 410                 415     


Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp 
            420                 425                 430         


Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val 
        435                 440                 445             


Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val 
    450                 455                 460                 


Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val 
465                 470                 475                 480 


Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg 
                485                 490                 495     


Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln 
            500                 505                 510         


Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu 
        515                 520                 525             


Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly 
    530                 535                 540                 


Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile 
545                 550                 555                 560 


Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu 
                565                 570                 575     


Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr 
            580                 585                 590         


Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly 
        595                 600                 605             


Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala 
    610                 615                 620                 


Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu 
625                 630                 635                 640 


Lys Leu Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr 
                645                 650         


<210>  28
<211>  624
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       N-terminal+K240+A376+C-terminal

<400>  28

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys 
        195                 200                 205             


Asp Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp 
    210                 215                 220                 


Thr Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys 
225                 230                 235                 240 


His Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe 
                245                 250                 255     


Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
            260                 265                 270         


Leu Pro Leu Glu Ala Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu 
        275                 280                 285             


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
    290                 295                 300                 


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
305                 310                 315                 320 


Ala Leu Ala Lys Asp Gln Asn Ala Thr Lys Pro Gly Ser Gly Gly Asp 
                325                 330                 335     


Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu 
            340                 345                 350         


Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly 
        355                 360                 365             


Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys 
    370                 375                 380                 


Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
385                 390                 395                 400 


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
                405                 410                 415     


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
            420                 425                 430         


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
        435                 440                 445             


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
    450                 455                 460                 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
465                 470                 475                 480 


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
                485                 490                 495     


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
            500                 505                 510         


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
        515                 520                 525             


Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly 
    530                 535                 540                 


His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu 
545                 550                 555                 560 


Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val 
                565                 570                 575     


Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp 
            580                 585                 590         


Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu 
        595                 600                 605             


Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615                 620                 


<210>  29
<211>  624
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       Y208+A519

<400>  29

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys 
        195                 200                 205             


Asp Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp 
    210                 215                 220                 


Thr Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys 
225                 230                 235                 240 


His Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe 
                245                 250                 255     


Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
            260                 265                 270         


Leu Pro Leu Glu Ala Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu 
        275                 280                 285             


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
    290                 295                 300                 


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
305                 310                 315                 320 


Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
                325                 330                 335     


Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
            340                 345                 350         


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
        355                 360                 365             


Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu 
    370                 375                 380                 


Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr 
385                 390                 395                 400 


Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser 
                405                 410                 415     


Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His 
            420                 425                 430         


Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr 
        435                 440                 445             


Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg 
    450                 455                 460                 


Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp 
465                 470                 475                 480 


Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg 
                485                 490                 495     


Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Trp 
            500                 505                 510         


Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Lys Asp Gln Asn 
        515                 520                 525             


Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly 
    530                 535                 540                 


His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu 
545                 550                 555                 560 


Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val 
                565                 570                 575     


Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp 
            580                 585                 590         


Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu 
        595                 600                 605             


Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615                 620                 


<210>  30
<211>  624
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       R274+S318

<400>  30

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Lys Asp Gln Asn Ala Thr Lys His Arg Gln Pro Arg Gly Trp Glu 
        275                 280                 285             


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
    290                 295                 300                 


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
305                 310                 315                 320 


Ala Leu Ala Lys Asp Gln Asn Ala Thr Lys Pro Gly Ser Gly Gly Asp 
                325                 330                 335     


Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu 
            340                 345                 350         


Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly 
        355                 360                 365             


Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys 
    370                 375                 380                 


Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala 
385                 390                 395                 400 


Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly 
                405                 410                 415     


Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu 
            420                 425                 430         


Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe 
        435                 440                 445             


Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe 
    450                 455                 460                 


Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly 
465                 470                 475                 480 


Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp 
                485                 490                 495     


Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg 
            500                 505                 510         


Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu 
        515                 520                 525             


Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile Gly 
    530                 535                 540                 


His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu 
545                 550                 555                 560 


Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr Val 
                565                 570                 575     


Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly Asp 
            580                 585                 590         


Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu 
        595                 600                 605             


Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615                 620                 


<210>  31
<211>  625
<212>  PRT
<213>  Artificial

<220>
<223>  Modified EPA sequence with consensus sequences inserted at 
       R274+A519

<400>  31

Ala Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys 
1               5                   10                  15      


Val Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp 
            20                  25                  30          


Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met 
        35                  40                  45              


Val Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala 
    50                  55                  60                  


Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val 
65                  70                  75                  80  


Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly 
                85                  90                  95      


Ser Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser 
            100                 105                 110         


Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser 
        115                 120                 125             


His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala 
    130                 135                 140                 


Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn 
145                 150                 155                 160 


Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val 
                165                 170                 175     


Met Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala 
            180                 185                 190         


Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn 
        195                 200                 205             


Tyr Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys 
    210                 215                 220                 


Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile 
225                 230                 235                 240 


Lys Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser 
                245                 250                 255     


Leu Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala 
            260                 265                 270         


Phe Thr Lys Asp Gln Asn Ala Thr Lys His Arg Gln Pro Arg Gly Trp 
        275                 280                 285             


Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu 
    290                 295                 300                 


Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg 
305                 310                 315                 320 


Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile 
                325                 330                 335     


Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala 
            340                 345                 350         


Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly 
        355                 360                 365             


Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly 
    370                 375                 380                 


Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn 
385                 390                 395                 400 


Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe 
                405                 410                 415     


Ser Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala 
            420                 425                 430         


His Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly 
        435                 440                 445             


Thr Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala 
    450                 455                 460                 


Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly 
465                 470                 475                 480 


Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala 
                485                 490                 495     


Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg 
            500                 505                 510         


Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Lys Asp Gln 
        515                 520                 525             


Asn Ala Thr Lys Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile 
    530                 535                 540                 


Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu 
545                 550                 555                 560 


Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr 
                565                 570                 575     


Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly 
            580                 585                 590         


Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala 
        595                 600                 605             


Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu 
    610                 615                 620                 


Lys 
625 


<210>  32
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  32
aagctagcgc cgccgaggaa gccttcgacc                                        30


<210>  33
<211>  50
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  33
aagaattctc agtggtggtg gtggtggtgc ttcaggtcct cgcgcggcgg                  50


<210>  34
<211>  618
<212>  PRT
<213>  Artificial

<220>
<223>  EPA_mut_Y208

<400>  34

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Lys 
        195                 200                 205             


Asp Gln Asn Ala Thr Lys Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp 
    210                 215                 220                 


Thr Trp Glu Gly Lys Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys 
225                 230                 235                 240 


His Asp Leu Asp Ile Lys Pro Thr Val Ile Ser His Arg Leu His Phe 
                245                 250                 255     


Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys His 
            260                 265                 270         


Leu Pro Leu Glu Thr Phe Thr Arg His Arg Gln Pro Arg Gly Trp Glu 
        275                 280                 285             


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
    290                 295                 300                 


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
305                 310                 315                 320 


Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
                325                 330                 335     


Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
            340                 345                 350         


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
        355                 360                 365             


Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu 
    370                 375                 380                 


Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr 
385                 390                 395                 400 


Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser 
                405                 410                 415     


Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His 
            420                 425                 430         


Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr 
        435                 440                 445             


Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg 
    450                 455                 460                 


Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp 
465                 470                 475                 480 


Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg 
                485                 490                 495     


Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Pro 
            500                 505                 510         


Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu 
        515                 520                 525             


Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg 
    530                 535                 540                 


Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile 
545                 550                 555                 560 


Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile 
                565                 570                 575     


Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile 
            580                 585                 590         


Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln 
        595                 600                 605             


Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615             


<210>  35
<211>  618
<212>  PRT
<213>  Artificial

<220>
<223>  EPA_mut_R274

<400>  35

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Lys Asp Gln Asn Ala Thr Lys His Arg Gln Pro Arg Gly Trp Glu 
        275                 280                 285             


Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr 
    290                 295                 300                 


Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg Asn 
305                 310                 315                 320 


Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
                325                 330                 335     


Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
            340                 345                 350         


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
        355                 360                 365             


Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu 
    370                 375                 380                 


Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr 
385                 390                 395                 400 


Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser 
                405                 410                 415     


Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His 
            420                 425                 430         


Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr 
        435                 440                 445             


Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg 
    450                 455                 460                 


Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp 
465                 470                 475                 480 


Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg 
                485                 490                 495     


Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Trp 
            500                 505                 510         


Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu 
        515                 520                 525             


Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg 
    530                 535                 540                 


Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile 
545                 550                 555                 560 


Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile 
                565                 570                 575     


Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile 
            580                 585                 590         


Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln 
        595                 600                 605             


Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615             


<210>  36
<211>  618
<212>  PRT
<213>  Artificial

<220>
<223>  EPA_mut_S318

<400>  36

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly 
        275                 280                 285             


Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser 
    290                 295                 300                 


Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Lys Asp Gln 
305                 310                 315                 320 


Asn Ala Thr Lys Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg 
                325                 330                 335     


Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu 
            340                 345                 350         


Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala 
        355                 360                 365             


Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Ala Gly Glu 
    370                 375                 380                 


Cys Ala Gly Pro Ala Asp Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr 
385                 390                 395                 400 


Pro Thr Gly Ala Glu Phe Leu Gly Asp Gly Gly Asp Val Ser Phe Ser 
                405                 410                 415     


Thr Arg Gly Thr Gln Asn Trp Thr Val Glu Arg Leu Leu Gln Ala His 
            420                 425                 430         


Arg Gln Leu Glu Glu Arg Gly Tyr Val Phe Val Gly Tyr His Gly Thr 
        435                 440                 445             


Phe Leu Glu Ala Ala Gln Ser Ile Val Phe Gly Gly Val Arg Ala Arg 
    450                 455                 460                 


Ser Gln Asp Leu Asp Ala Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp 
465                 470                 475                 480 


Pro Ala Leu Ala Tyr Gly Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg 
                485                 490                 495     


Gly Arg Ile Arg Asn Gly Ala Leu Leu Arg Val Tyr Val Pro Arg Trp 
            500                 505                 510         


Ser Leu Pro Gly Phe Tyr Arg Thr Gly Leu Thr Leu Ala Ala Pro Glu 
        515                 520                 525             


Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg 
    530                 535                 540                 


Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile 
545                 550                 555                 560 


Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile 
                565                 570                 575     


Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile 
            580                 585                 590         


Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln 
        595                 600                 605             


Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615             


<210>  37
<211>  618
<212>  PRT
<213>  Artificial

<220>
<223>  EPA_mut_A519

<400>  37

Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys Val 
1               5                   10                  15      


Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp Pro 
            20                  25                  30          


Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met Val 
        35                  40                  45              


Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala Leu 
    50                  55                  60                  


Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val Glu 
65                  70                  75                  80  


Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly Ser 
                85                  90                  95      


Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser Asn 
            100                 105                 110         


Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser His 
        115                 120                 125             


Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala Lys 
    130                 135                 140                 


Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn Glu 
145                 150                 155                 160 


Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val Met 
                165                 170                 175     


Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala Ser 
            180                 185                 190         


Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn Tyr 
        195                 200                 205             


Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys Ile 
    210                 215                 220                 


Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile Lys 
225                 230                 235                 240 


Pro Thr Val Ile Ser His Arg Leu His Phe Pro Glu Gly Gly Ser Leu 
                245                 250                 255     


Ala Ala Leu Thr Ala His Gln Ala Cys His Leu Pro Leu Glu Ala Phe 
            260                 265                 270         


Thr Arg His Arg Gln Pro Arg Gly Trp Glu Gln Leu Glu Gln Cys Gly 
        275                 280                 285             


Tyr Pro Val Gln Arg Leu Val Ala Leu Tyr Leu Ala Ala Arg Leu Ser 
    290                 295                 300                 


Trp Asn Gln Val Asp Gln Val Ile Arg Asn Ala Leu Ala Ser Pro Gly 
305                 310                 315                 320 


Ser Gly Gly Asp Leu Gly Glu Ala Ile Arg Glu Gln Pro Glu Gln Ala 
                325                 330                 335     


Arg Leu Ala Leu Thr Leu Ala Ala Ala Glu Ser Glu Arg Phe Val Arg 
            340                 345                 350         


Gln Gly Thr Gly Asn Asp Glu Ala Gly Ala Ala Ser Ala Asp Val Val 
        355                 360                 365             


Ser Leu Thr Cys Pro Val Ala Ala Gly Glu Cys Ala Gly Pro Ala Asp 
    370                 375                 380                 


Ser Gly Asp Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe 
385                 390                 395                 400 


Leu Gly Asp Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn 
                405                 410                 415     


Trp Thr Val Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg 
            420                 425                 430         


Gly Tyr Val Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln 
        435                 440                 445             


Ser Ile Val Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala 
    450                 455                 460                 


Ile Trp Arg Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly 
465                 470                 475                 480 


Tyr Ala Gln Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly 
                485                 490                 495     


Ala Leu Leu Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr 
            500                 505                 510         


Arg Thr Gly Leu Thr Leu Lys Asp Gln Asn Ala Thr Lys Ala Pro Glu 
        515                 520                 525             


Ala Ala Gly Glu Val Glu Arg Leu Ile Gly His Pro Leu Pro Leu Arg 
    530                 535                 540                 


Leu Asp Ala Ile Thr Gly Pro Glu Glu Glu Gly Gly Arg Val Thr Ile 
545                 550                 555                 560 


Leu Gly Trp Pro Leu Ala Glu Arg Thr Val Val Ile Pro Ser Ala Ile 
                565                 570                 575     


Pro Thr Asp Pro Arg Asn Val Gly Gly Asp Leu Asp Pro Ser Ser Ile 
            580                 585                 590         


Pro Asp Lys Glu Gln Ala Ile Ser Ala Leu Pro Asp Tyr Ala Ser Gln 
        595                 600                 605             


Pro Gly Lys Pro Pro Arg Glu Asp Leu Lys 
    610                 615             


<210>  38
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  38
gaaggcgggc gcgtgaccat tctcggc                                           27


<210>  39
<211>  27
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  39
gccgagaatg gtcacgcgcc cgccttc                                           27


<210>  40
<211>  1932
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence EPA with mutation Y208 > KDQNATK

<400>  40
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc tagcgccgcc       60

gaggaagcct tcgacctctg gaacgaatgc gccaaggcct gcgtgctcga cctcaaggac      120

ggcgtgcgtt ccagccgcat gagcgtcgac ccggccatcg ccgacaccaa cggccagggc      180

gtgctgcact actccatggt cctggagggc ggcaacgacg cgctcaagct ggccatcgac      240

aacgccctca gcatcaccag cgacggcctg accatccgcc tcgaaggtgg cgtcgagccg      300

aacaagccgg tgcgctacag ctacacgcgc caggcgcgcg gcagttggtc gctgaactgg      360

ctggtgccga tcggccacga gaagccttcg aacatcaagg tgttcatcca cgaactgaac      420

gccggtaacc agctcagcca catgtcgccg atctacacca tcgagatggg cgacgagttg      480

ctggcgaagc tggcgcgcga tgccaccttc ttcgtcaggg cgcacgagag caacgagatg      540

cagccgacgc tcgccatcag ccatgccggg gtcagcgtgg tcatggccca ggcccagccg      600

cgccgggaaa agcgctggag cgaatgggcc agcggcaagg tgttgtgcct gctcgacccg      660

ctggacgggg tctacaacaa agatcagaac gcgaccaaac tcgcccagca gcgctgcaac      720

ctcgacgata cctgggaagg caagatctac cgggtgctcg ccggcaaccc ggcgaagcat      780

gacctggaca tcaagcccac ggtcatcagt catcgcctgc atttccccga gggcggcagc      840

ctggccgcgc tgaccgcgca ccaggcctgc cacctgccgc tggagacctt cacccgtcat      900

cgccagccgc gcggctggga acaactggag cagtgcggct atccggtgca gcggctggtc      960

gccctctacc tggcggcgcg gctgtcgtgg aaccaggtcg accaggtgat ccgcaacgcc     1020

ctggccagcc ccggcagcgg cggcgacctg ggcgaagcga tccgcgagca gccggagcag     1080

gcccgtctgg ccctgaccct ggccgccgcc gagagcgagc gcttcgtccg gcagggcacc     1140

ggcaacgacg aggccggcgc ggccagcgcc gacgtggtga gcctgacctg cccggtcgcc     1200

gccggtgaat gcgcgggccc ggcggacagc ggcgacgccc tgctggagcg caactatccc     1260

actggcgcgg agttcctcgg cgacggcggc gacgtcagct tcagcacccg cggcacgcag     1320

aactggacgg tggagcggct gctccaggcg caccgccaac tggaggagcg cggctatgtg     1380

ttcgtcggct accacggcac cttcctcgaa gcggcgcaaa gcatcgtctt cggcggggtg     1440

cgcgcgcgca gccaggacct cgacgcgatc tggcgcggtt tctatatcgc cggcgatccg     1500

gcgctggcct acggctacgc ccaggaccag gaacccgacg cgcgcggccg gatccgcaac     1560

ggtgccctgc tgcgggtcta tgtgccgcgc ccgagtctgc cgggcttcta ccgcaccggc     1620

ctgaccctgg ccgcgccgga ggcggcgggc gaggtcgaac ggctgatcgg ccatccgctg     1680

ccgctgcgcc tggacgccat caccggcccc gaggaggaag gcgggcgcgt gaccattctc     1740

ggctggccgc tggccgagcg caccgtggtg attccctcgg cgatccccac cgacccgcgc     1800

aacgtcggcg gcgacctcga cccgtccagc atccccgaca aggaacaggc gatcagcgcc     1860

ctgccggact acgccagcca gcccggcaaa ccgccgcgcg aggacttgaa gcaccaccac     1920

caccaccact ga                                                         1932


<210>  41
<211>  1932
<212>  DNA
<213>  Artificial

<220>
<223>  SEQ ID NO: 41 Nucleotide sequence EPA with mutation R274 > 
       KDQNATK

<400>  41
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc tagcgccgcc       60

gaggaagcct tcgacctctg gaacgaatgc gccaaggcct gcgtgctcga cctcaaggac      120

ggcgtgcgtt ccagccgcat gagcgtcgac ccggccatcg ccgacaccaa cggccagggc      180

gtgctgcact actccatggt cctggagggc ggcaacgacg cgctcaagct ggccatcgac      240

aacgccctca gcatcaccag cgacggcctg accatccgcc tcgaaggcgg cgtcgagccg      300

aacaagccgg tgcgctacag ctacacgcgc caggcgcgcg gcagttggtc gctgaactgg      360

ctggtaccga tcggccacga gaagccctcg aacatcaagg tgttcatcca cgaactgaac      420

gccggtaacc agctcagcca catgtcgccg atctacacca tcgagatggg cgacgagttg      480

ctggcgaagc tggcgcgcga tgccaccttc ttcgtcaggg cgcacgagag caacgagatg      540

cagccgacgc tcgccatcag ccatgccggg gtcagcgtgg tcatggctca ggcccagccg      600

cgccgggaaa agcgctggag cgaatgggcc agcggcaagg tgttgtgcct gctcgacccg      660

ctggacgggg tctacaacta cctcgcccag cagcgctgca acctcgacga tacctgggaa      720

ggcaagatct accgggtgct cgccggcaac ccggcgaagc atgacctgga catcaagccc      780

acggtcatca gtcatcgcct gcatttcccc gagggcggca gcctggccgc gctgaccgcg      840

caccaggcct gccacctgcc gctggaggcc ttcactaaag atcagaacgc gaccaaacat      900

cgccagccgc gcggctggga acaactggag cagtgcggct atccggtgca gcggctggtc      960

gccctctacc tggcggcgcg actgtcgtgg aaccaggtcg accaggtgat ccgcaacgcc     1020

ctggccagcc ccggcagcgg cggcgacctg ggcgaagcga tccgcgagca gccggagcag     1080

gcccgtctgg ccctgaccct ggccgccgcc gagagcgagc gcttcgtccg gcagggcacc     1140

ggcaacgacg aggccggcgc ggccagcgcc gacgtggtga gcctgacctg cccggtcgcc     1200

gccggtgaat gcgcgggccc ggcggacagc ggcgacgccc tgctggagcg caactatccc     1260

actggcgcgg agttcctcgg cgacggcggc gacgtcagct tcagcacccg cggcacgcag     1320

aactggacgg tggagcggct gctccaggcg caccgccaac tggaggagcg cggctatgtg     1380

ttcgtcggct accacggcac cttcctcgaa gcggcgcaaa gcatcgtctt cggcggggtg     1440

cgcgcgcgca gccaggacct cgacgcgatc tggcgcggtt tctatatcgc cggcgatccg     1500

gcgctggcct acggctacgc ccaggaccag gaacccgacg cgcgcggccg gatccgcaac     1560

ggtgccctgc tgcgggtcta tgtgccgcgc tggagtctgc cgggcttcta ccgcaccggc     1620

ctgaccctgg ccgcgccgga ggcggcgggc gaggtcgaac ggctgatcgg ccatccgctg     1680

ccgctgcgcc tggacgccat caccggcccc gaggaggaag gcgggcgcgt gaccattctc     1740

ggctggccgc tggccgagcg caccgtggtg attccctcgg cgatccccac cgacccgcgc     1800

aacgtcggcg gcgacctcga cccgtccagc atccccgaca aggaacaggc gatcagcgcc     1860

ctgccggact acgccagcca gcccggcaaa ccgccgcgcg aggacttgaa gcaccaccac     1920

caccaccact ga                                                         1932


<210>  42
<211>  1932
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence EPA with mutation S318 > KDQNATK

<400>  42
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc tagcgccgcc       60

gaggaagcct tcgacctctg gaacgaatgc gccaaggcct gcgtgctcga cctcaaggac      120

ggcgtgcgtt ccagccgcat gagcgtcgac ccggccatcg ccgacaccaa cggccagggc      180

gtgctgcact actccatggt cctggagggc ggcaacgacg cgctcaagct ggccatcgac      240

aacgccctca gcatcaccag cgacggcctg accatccgcc tcgaaggcgg cgtcgagccg      300

aacaagccgg tgcgctacag ctacacgcgc caggcgcgcg gcagttggtc gctgaactgg      360

ctggtaccga tcggccacga gaagccctcg aacatcaagg tgttcatcca cgaactgaac      420

gccggtaacc agctcagcca catgtcgccg atctacacca tcgagatggg cgacgagttg      480

ctggcgaagc tggcgcgcga tgccaccttc ttcgtcaggg cgcacgagag caacgagatg      540

cagccgacgc tcgccatcag ccatgccggg gtcagcgtgg tcatggctca ggcccagccg      600

cgccgggaaa agcgctggag cgaatgggcc agcggcaagg tgttgtgcct gctcgacccg      660

ctggacgggg tctacaacta cctcgcccag cagcgctgca acctcgacga tacctgggaa      720

ggcaagatct accgggtgct cgccggcaac ccggcgaagc atgacctgga catcaagccc      780

acggtcatca gtcatcgcct gcatttcccc gagggcggca gcctggccgc gctgaccgcg      840

caccaggcct gccacctgcc gctggaggcc ttcactcgtc atcgccagcc gcgcggctgg      900

gaacaactgg agcagtgcgg ctatccggtg cagcggctgg tcgccctcta cctggcggcg      960

cgactgtcgt ggaaccaggt cgaccaggtg atccgcaacg ccctggccaa agatcagaac     1020

gcgaccaaac ccggcagcgg cggcgacctg ggcgaagcga tccgcgagca gccggagcag     1080

gcccgtctgg ccctgaccct ggccgccgcc gagagcgagc gcttcgtccg gcagggcacc     1140

ggcaacgacg aggccggcgc ggccagcgcc gacgtggtga gcctgacctg cccggtcgcc     1200

gccggtgaat gcgcgggccc ggcggacagc ggcgacgccc tgctggagcg caactatccc     1260

actggcgcgg agttcctcgg cgacggcggc gacgtcagct tcagcacccg cggcacgcag     1320

aactggacgg tggagcggct gctccaggcg caccgccaac tggaggagcg cggctatgtg     1380

ttcgtcggct accacggcac cttcctcgaa gcggcgcaaa gcatcgtctt cggcggggtg     1440

cgcgcgcgca gccaggacct cgacgcgatc tggcgcggtt tctatatcgc cggcgatccg     1500

gcgctggcct acggctacgc ccaggaccag gaacccgacg cgcgcggccg gatccgcaac     1560

ggtgccctgc tgcgggtcta tgtgccgcgc tggagtctgc cgggcttcta ccgcaccggc     1620

ctgaccctgg ccgcgccgga ggcggcgggc gaggtcgaac ggctgatcgg ccatccgctg     1680

ccgctgcgcc tggacgccat caccggcccc gaggaggaag gcgggcgcgt gaccattctc     1740

ggctggccgc tggccgagcg caccgtggtg attccctcgg cgatccccac cgacccgcgc     1800

aacgtcggcg gcgacctcga cccgtccagc atccccgaca aggaacaggc gatcagcgcc     1860

ctgccggact acgccagcca gcccggcaaa ccgccgcgcg aggacttgaa gcaccaccac     1920

caccaccact ga                                                         1932


<210>  43
<211>  1932
<212>  DNA
<213>  Artificial

<220>
<223>  Nucleotide sequence EPA with mutation A519 > KDQNATK

<400>  43
atgaaaaaga tttggctggc gctggctggt ttagttttag cgtttagcgc tagcgccgcc       60

gaggaagcct tcgacctctg gaacgaatgc gccaaggcct gcgtgctcga cctcaaggac      120

ggcgtgcgtt ccagccgcat gagcgtcgac ccggccatcg ccgacaccaa cggccagggc      180

gtgctgcact actccatggt cctggagggc ggcaacgacg cgctcaagct ggccatcgac      240

aacgccctca gcatcaccag cgacggcctg accatccgcc tcgaaggcgg cgtcgagccg      300

aacaagccgg tgcgctacag ctacacgcgc caggcgcgcg gcagttggtc gctgaactgg      360

ctggtaccga tcggccacga gaagccctcg aacatcaagg tgttcatcca cgaactgaac      420

gccggtaacc agctcagcca catgtcgccg atctacacca tcgagatggg cgacgagttg      480

ctggcgaagc tggcgcgcga tgccaccttc ttcgtcaggg cgcacgagag caacgagatg      540

cagccgacgc tcgccatcag ccatgccggg gtcagcgtgg tcatggctca ggcccagccg      600

cgccgggaaa agcgctggag cgaatgggcc agcggcaagg tgttgtgcct gctcgacccg      660

ctggacgggg tctacaacta cctcgcccag cagcgctgca acctcgacga tacctgggaa      720

ggcaagatct accgggtgct cgccggcaac ccggcgaagc atgacctgga catcaagccc      780

acggtcatca gtcatcgcct gcatttcccc gagggcggca gcctggccgc gctgaccgcg      840

caccaggcct gccacctgcc gctggaggcc ttcactcgtc atcgccagcc gcgcggctgg      900

gaacaactgg agcagtgcgg ctatccggtg cagcggctgg tcgccctcta cctggcggcg      960

cgactgtcgt ggaaccaggt cgaccaggtg atccgcaacg ccctggccag ccccggcagc     1020

ggcggcgacc tgggcgaagc gatccgcgag cagccggagc aggcccgtct ggccctgacc     1080

ctggccgccg ccgagagcga gcgcttcgtc cggcagggca ccggcaacga cgaggccggc     1140

gcggccagcg ccgacgtggt gagcctgacc tgcccggtcg ccgccggtga atgcgcgggc     1200

ccggcggaca gcggcgacgc cctgctggag cgcaactatc ccactggcgc ggagttcctc     1260

ggcgacggcg gcgacgtcag cttcagcacc cgcggcacgc agaactggac ggtggagcgg     1320

ctgctccagg cgcaccgcca actggaggag cgcggctatg tgttcgtcgg ctaccacggc     1380

accttcctcg aagcggcgca aagcatcgtc ttcggcgggg tgcgcgcgcg cagccaggac     1440

ctcgacgcga tctggcgcgg tttctatatc gccggcgatc cggcgctggc ctacggctac     1500

gcccaggacc aggaacccga cgcgcgcggc cggatccgca acggtgccct gctgcgggtc     1560

tatgtgccgc gctggagtct gccgggcttc taccgcaccg gcctgaccct gaaagatcag     1620

aacgcgacca aagcgccgga ggcggcgggc gaggtcgaac ggctgatcgg ccatccgctg     1680

ccgctgcgcc tggacgccat caccggcccc gaggaggaag gcgggcgcgt gaccattctc     1740

ggctggccgc tggccgagcg caccgtggtg attccctcgg cgatccccac cgacccgcgc     1800

aacgtcggcg gcgacctcga cccgtccagc atccccgaca aggaacaggc gatcagcgcc     1860

ctgccggact acgccagcca gcccggcaaa ccgccgcgcg aggacttgaa gcaccaccac     1920

caccaccact ga                                                         1932


