<110>    Korea Research Institute of Chemical Technology

<120>    fluorescent protein-tagged cytochrome P450 transformed human
         induced pluripotent stem cells and screening method of AHR
         regulator using the same

<130>    2021FPO-01-009/PCT

<150>    KR 1020200075214
<151>    2020-06-19

<160>    39

<170>    KoPatentIn 3.0

<210>    1
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    single guide RNA 1


<400>    1
gcattgatcc tcctgtccat ggg                                                 23


<210>    2
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    single guide RNA 4


<400>    2
taggtagtgg ctcccttcaa agg                                                 23


<210>    3
<211>    23
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    single guide RNA 5


<400>    3
ctggcactga cccctttgaa ggg                                                 23


<210>    4
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CYP1A1 forward


<400>    4
aggcttttac atccccaagg                                                     20


<210>    5
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    CYP1A1 reverse


<400>    5
ttgtcgatag caccatcagg                                                     20


<210>    6
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    mCherry forward


<400>    6
gtgagcactt ccaaatgcag c                                                   21


<210>    7
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    mCherry reverse


<400>    7
ccttgaagcg catgaactcc t                                                   21


<210>    8
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    PAX6 forward


<400>    8
gtgtccaacg gatgtgtgag                                                     20


<210>    9
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    PAX6 reverse


<400>    9
ctagccaggt tgcgaagaac                                                     20


<210>    10
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    OTX2 forward


<400>    10
tgcaggggtt cttctgtgat                                                     20


<210>    11
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    OTX2 reverse


<400>    11
agggtcagag caattgacca                                                     20


<210>    12
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    alpha SMA forward


<400>    12
tgctctgggt tcgtcagagt c                                                   21


<210>    13
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    alpha SMA reverse


<400>    13
caggcaagtc actgtgtggc                                                     20


<210>    14
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    EOMES forward


<400>    14
aggcgcaaat aacaacaaca c                                                   21


<210>    15
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    EOMES reverse


<400>    15
attcaagtcc tccacgccat c                                                   21


<210>    16
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Brachyury forward


<400>    16
gcgggaaaga gcctgcagta                                                     20


<210>    17
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Brachyury reverse


<400>    17
ttccccgttc acgtacttcc                                                     20


<210>    18
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    FOXA2 forward


<400>    18
atgcactcgg cttccagtat                                                     20


<210>    19
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    FOXA2 reverse


<400>    19
cacgtacgac gacatgttca                                                     20


<210>    20
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GATA4 forward


<400>    20
tccaaaccag aaaacggaag                                                     20


<210>    21
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    GATA4 reverse


<400>    21
ctgtgcccgt agtgagatga                                                     20


<210>    22
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    SOX17 forward


<400>    22
cagaatccag acctgcacaa                                                     20


<210>    23
<211>    19
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    SOX17 reverse


<400>    23
gcggccggta cttgtagtt                                                      19


<210>    24
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    ALB forward


<400>    24
gagaccagag gttgatgtga tg                                                  22


<210>    25
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    ALB reverse


<400>    25
agttccgggg cataaaagta ag                                                  22


<210>    26
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    AAT forward


<400>    26
gaagtcaagg acaccgagga                                                     20


<210>    27
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    AAT reverse


<400>    27
gctggcagac cttctgtctt                                                     20


<210>    28
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TDO2 forward


<400>    28
caaatcctct gggagttgga                                                     20


<210>    29
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TDO2 reverse


<400>    29
gtccaaggct gtcatcgtct                                                     20


<210>    30
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    HNF4A forward


<400>    30
cgagcagatc cagttcatca                                                     20


<210>    31
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    HNF4A reverse


<400>    31
tcacacatct gtccgttgct                                                     20


<210>    32
<211>    25
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    G6Pase forward


<400>    32
tcaacctcgt ctttaagtgg attct                                               25


<210>    33
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    G6Pase reverse


<400>    33
agtatacacc tgctgtgccc                                                     20


<210>    34
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TAT forward


<400>    34
gcttcctcaa gtccaatgct                                                     20


<210>    35
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TAT reverse


<400>    35
ctgtgatgac cactcggatg                                                     20


<210>    36
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TTR forward


<400>    36
accggtgaat ccaagtgtcc                                                     20


<210>    37
<211>    21
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    TTR reverse


<400>    37
ggttttccca gaggcaaatg g                                                   21


<210>    38
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    AFP forward


<400>    38
agcttggtgg tggatgaa                                                       18


<210>    39
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    AFP reverse


<400>    39
tctgcaatga cagcctcaag                                                     20
