                         SEQUENCE LISTING

<110>  Ziphius Vaccines
 
<120>  SELF-AMPLIFYING  SARS-COV-2  RNA VACCINE

<130>  ZIPH-001-EP3-WO

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RBD of Spike protein from SARS-CoV-2

<400>  1

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Arg Val Gln Pro Thr Glu Ser Ile Val Arg 
            20                  25                  30          


Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
        35                  40                  45              


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
    50                  55                  60                  


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
65                  70                  75                  80  


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
                85                  90                  95      


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
            100                 105                 110         


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
        115                 120                 125             


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
    130                 135                 140                 


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
145                 150                 155                 160 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
                165                 170                 175     


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
            180                 185                 190         


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
        195                 200                 205             


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
    210                 215                 220                 


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
225                 230                 235                 240 


Lys Cys Val Asn Phe 
                245 


<210>  2
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fusion protein RBD and C3d-p28.6

<400>  2

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Arg Val Gln Pro Thr Glu Ser Ile Val Arg 
            20                  25                  30          


Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
        35                  40                  45              


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
    50                  55                  60                  


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
65                  70                  75                  80  


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
                85                  90                  95      


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
            100                 105                 110         


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
        115                 120                 125             


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
    130                 135                 140                 


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
145                 150                 155                 160 


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
                165                 170                 175     


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
            180                 185                 190         


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
        195                 200                 205             


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
    210                 215                 220                 


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
225                 230                 235                 240 


Lys Cys Val Asn Phe Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Lys 
                245                 250                 255     


Phe Leu Thr Thr Ala Lys Asp Lys Asn Arg Trp Glu Asp Pro Gly Lys 
            260                 265                 270         


Gln Leu Tyr Asn Val Glu Ala Thr Ser Tyr Ala Gly Gly Gly Gly Ser 
        275                 280                 285             


Gly Gly Gly Gly Ser Lys Phe Leu Thr Thr Ala Lys Asp Lys Asn Arg 
    290                 295                 300                 


Trp Glu Asp Pro Gly Lys Gln Leu Tyr Asn Val Glu Ala Thr Ser Tyr 
305                 310                 315                 320 


Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Lys Phe Leu Thr Thr 
                325                 330                 335     


Ala Lys Asp Lys Asn Arg Trp Glu Asp Pro Gly Lys Gln Leu Tyr Asn 
            340                 345                 350         


Val Glu Ala Thr Ser Tyr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        355                 360                 365             


Ser Lys Phe Leu Thr Thr Ala Lys Asp Lys Asn Arg Trp Glu Asp Pro 
    370                 375                 380                 


Gly Lys Gln Leu Tyr Asn Val Glu Ala Thr Ser Tyr Ala Gly Gly Gly 
385                 390                 395                 400 


Gly Ser Gly Gly Gly Gly Ser Lys Phe Leu Thr Thr Ala Lys Asp Lys 
                405                 410                 415     


Asn Arg Trp Glu Asp Pro Gly Lys Gln Leu Tyr Asn Val Glu Ala Thr 
            420                 425                 430         


Ser Tyr Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Lys Phe Leu 
        435                 440                 445             


Thr Thr Ala Lys Asp Lys Asn Arg Trp Glu Asp Pro Gly Lys Gln Leu 
    450                 455                 460                 


Tyr Asn Val Glu Ala Thr Ser Tyr Ala 
465                 470             


<210>  3
<211>  738
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of S-RBD

<400>  3
atggatgcta tgaagagggg cctgtgctgc gtgctgcttc tgtgtggcgc tgtgttcgtg       60

tcccctagag tgcagcctac cgagagcatc gtgcggttcc ccaacatcac caatctgtgc      120

cctttcggcg aggtgttcaa cgccaccaga ttcgcctctg tgtacgcctg gaaccggaag      180

cggatcagca attgcgtggc cgactacagc gtgctgtaca acagcgccag cttcagcacc      240

ttcaagtgct acggcgtgtc acccaccaag ctgaacgacc tgtgcttcac caacgtgtac      300

gccgacagct tcgtgatcag aggcgacgaa gtgcggcaga ttgcccctgg acagacaggc      360

aagatcgccg attacaacta caagctgccc gacgacttca ccggctgtgt gattgcctgg      420

aacagcaaca acctggacag caaagtcggc ggcaactaca actacctgta ccggctgttc      480

cggaagtcca acctgaagcc tttcgagcgg gacatcagca ccgagatcta tcaggccggc      540

agcacccctt gcaatggcgt ggaaggcttc aactgctact tcccactgca gtcctacggc      600

ttccagccta caaacggcgt gggctaccag ccttacagag tggtggtgct gagcttcgag      660

ctgctgcatg ctcctgccac agtgtgcggc cctaagaaaa gcaccaacct ggtcaagaac      720

aaatgcgtga acttctga                                                    738


<210>  4
<211>  1422
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of S-RBD-p28.6

<400>  4
atggatgcta tgaagagggg cctgtgctgc gtgctgcttc tgtgtggcgc tgtgttcgtg       60

tcccctagag tgcagcctac cgagagcatc gtgcggttcc ccaacatcac caatctgtgc      120

cctttcggcg aggtgttcaa cgccaccaga ttcgcctctg tgtacgcctg gaaccggaag      180

cggatcagca attgcgtggc cgactacagc gtgctgtaca acagcgccag cttcagcacc      240

ttcaagtgct acggcgtgtc acccaccaag ctgaacgacc tgtgcttcac caacgtgtac      300

gccgacagct tcgtgatcag aggcgacgaa gtgcggcaga ttgcccctgg acagacaggc      360

aagatcgccg attacaacta caagctgccc gacgacttca ccggctgtgt gattgcctgg      420

aacagcaaca acctggacag caaagtcggc ggcaactaca actacctgta ccggctgttc      480

cggaagtcca acctgaagcc tttcgagcgg gacatcagca ccgagatcta tcaggccggc      540

agcacccctt gcaatggcgt ggaaggcttc aactgctact tcccactgca gtcctacggc      600

ttccagccta caaacggcgt gggctaccag ccttacagag tggtggtgct gagcttcgag      660

ctgctgcatg ctcctgccac agtgtgcggc cctaagaaaa gcaccaacct ggtcaagaac      720

aaatgcgtga acttcggcgg aggcggaagt ggtggcggcg gatctaagtt tctgaccacc      780

gccaaggaca agaacagatg ggaagatccc ggcaagcagc tgtacaatgt ggaagccaca      840

agctacgcag gcggcggagg aagcggaggc ggaggtagta aatttctgac aacggctaaa      900

gataagaatc gctgggaaga tcctgggaaa cagctctata acgtcgaggc caccagctat      960

gctggcggtg gcggatctgg cggcggtggt tcaaaattcc tgactacagc caaggataag     1020

aatcgttggg aagatccagg caagcaactc tataatgttg aggctacctc ttacgctggt     1080

ggcggaggtt ctggcggcgg aggctctaaa tttctcacaa cagcaaagga caagaatcga     1140

tgggaagatc cgggaaaaca actgtacaac gttgaggcaa catcctatgc aggcggaggt     1200

ggcagtggcg gaggtggaag caagtttctg actactgcaa aagataagaa tagatgggaa     1260

gatcccggga agcaactcta caacgtcgaa gctactagtt atgccggtgg cggtggatct     1320

ggcggaggcg gcagcaaatt cctgaccacc gctaaagaca agaatcgttg ggaagatccc     1380

ggaaagcagt tgtataacgt tgaagctacg tcctacgcct ga                        1422


<210>  5
<211>  1260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of SARS-CoV-2 nucleocapsid

<400>  5
atgagcgaca acggccctca gaaccagaga aacgcccctc ggatcacatt tggcggccct       60

agcgatagca ccggcagcaa tcagaatggc gagagaagcg gcgccagaag caagcagaga      120

aggcctcaag gcctgcctaa caacaccgcc agctggttca cagccctgac acagcacggc      180

aaagaggacc tgaagttccc tagaggacag ggcgtgccca tcaacaccaa cagcagcccc      240

gatgaccaga tcggctacta cagacgggcc accagaagaa tcagaggcgg cgacggcaag      300

atgaaggatc tgagccccag atggtacttc tactacctcg gcacaggacc cgaagccgga      360

cttccttatg gcgccaacaa ggacggcatc atctgggttg caacagaagg cgccctgaac      420

acccctaagg accacatcgg caccagaaat cccgccaaca atgccgccat tgtgctgcag      480

ttgcctcagg gcacaacact gcccaagggc ttttacgccg agggctctag aggcggatct      540

caggccagca gcagaagcag ctccagatcc agaaacagct cccggaatag cacccctggc      600

tccagcagag gaacaagccc tgctagaatg gccggcaacg gcggagatgc tgctctggca      660

cttctcctgc tggaccggct gaatcagctg gaaagcaaga tgagcggcaa gggacagcag      720

cagcagggcc agaccgtgac aaaaaagtct gccgccgagg ccagcaagaa gcccagacag      780

aaaagaaccg ccaccaaggc ctacaacgtg acccaggcct ttggcagaag aggccctgag      840

cagacccagg gcaatttcgg cgatcaagag ctgatcagac agggcaccga ctacaagcac      900

tggcctcaga tcgcccagtt tgccccatct gccagcgcct ttttcggcat gagccggatc      960

ggcatggaag tgacacctag cggcacctgg ctgacataca caggcgccat caagctggac     1020

gacaaggacc ccaacttcaa ggaccaagtg atcctgctga acaagcacat cgacgcctac     1080

aagacattcc ctccaaccga gcctaagaag gacaagaaga agaaggccga cgagacacag     1140

gccctgcctc agcgccagaa aaagcagcag acagtgacac tgctgccagc cgccgacctg     1200

gacgattttt ctaagcagct gcagcagagc atgagcagcg ccgattctac acaggcctga     1260


<210>  6
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of SARS-CoV-2 membrane protein

<400>  6
atggccgata gcaatggcac catcaccgtg gaagaactga agaaactgct ggaacagtgg       60

aacctcgtga tcggcttcct gttcctgacc tggatctgcc tgctgcagtt cgcctacgcc      120

aaccggaaca gattcctgta tattatcaag ctgatcttcc tgtggctgct gtggcccgtg      180

acactggcct gttttgtgct ggccgccgtg taccggatca actggatcac aggcggaatc      240

gccattgcca tggcctgtct cgttggcctg atgtggctga gctactttat cgccagcttc      300

cggctgttcg cccggaccag atccatgtgg tccttcaatc ccgagacaaa catcctgctg      360

aacgtgcccc tgcacggcac catccttaca agacctctgc tggaaagcga gctggtcatc      420

ggagccgtga tcctgagagg ccacctgaga attgccggac accacctggg cagatgcgac      480

atcaaggacc tgcctaaaga aatcacagtg gccaccagca gaaccctgtc ctactataag      540

ctgggcgcca gccagagagt ggccggcgat tctggatttg ccgcctacag cagataccgg      600

atcggcaact acaagctgaa caccgaccac agctccagca gcgacaatat cgcactgctg      660

gtgcagtga                                                              669


<210>  7
<211>  9372
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of RNA replicon encoding SARS-CoV-2 
       S-RBD-C3d-p28.6

<400>  7
atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg       60

ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg      120

aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc      180

tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa      240

gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat      300

gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg      360

aaataactga taaggaattg gacaagaaaa tgaaggagct cgccgccgtc atgagcgacc      420

ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc      480

aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag      540

ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta      600

agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa      660

cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt      720

ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga      780

ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact      840

tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg      900

tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta      960

cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg     1020

tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac     1080

tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta     1140

tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg     1200

tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa     1260

ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc     1320

acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg     1380

atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa     1440

caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg     1500

acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt     1560

tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg     1620

tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa     1680

aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg     1740

ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga     1800

taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg     1860

tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca     1920

ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag     1980

gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg     2040

aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag     2100

ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa     2160

cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag     2220

gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga     2280

aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg     2340

ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata     2400

ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac     2460

ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc     2520

tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc     2580

gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa     2640

cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc     2700

aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca     2760

aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg     2820

ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg     2880

tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga     2940

taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag     3000

cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc     3060

agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca     3120

tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact     3180

cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg     3240

gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc     3300

cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc     3360

cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc     3420

gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag     3480

tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg     3540

gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt     3600

tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg     3660

tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc     3720

agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc     3780

tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa     3840

gcatcattgg tgctatagcg cggctgttca agttttcccg ggtatgcaaa ccgaaatcct     3900

cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc     3960

acaatcctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg     4020

aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag     4080

gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc     4140

tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac     4200

tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt     4260

cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca     4320

acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga     4380

acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg     4440

cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg     4500

ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg     4560

atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca     4620

caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg     4680

atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca     4740

tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg     4800

aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa     4860

gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat     4920

tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct     4980

caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag     5040

acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac     5100

cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg     5160

aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg     5220

aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat     5280

ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca     5340

gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc     5400

gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa     5460

gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc     5520

caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc     5580

ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga     5640

ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg     5700

catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa     5760

cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc     5820

tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta     5880

acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta     5940

ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc     6000

tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg     6060

cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta     6120

ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca     6180

ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac     6240

ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag     6300

ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg     6360

cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt     6420

ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa     6480

aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca     6540

taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa     6600

aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag     6660

cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga     6720

acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact     6780

tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg     6840

acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt     6900

tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta     6960

aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag     7020

tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg     7080

cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag     7140

acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga     7200

aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc     7260

gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg     7320

aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg     7380

gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca     7440

tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag     7500

gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa     7560

ggccaccatg gatgctatga agaggggcct gtgctgcgtg ctgcttctgt gtggcgctgt     7620

gttcgtgtcc cctagagtgc agcctaccga gagcatcgtg cggttcccca acatcaccaa     7680

tctgtgccct ttcggcgagg tgttcaacgc caccagattc gcctctgtgt acgcctggaa     7740

ccggaagcgg atcagcaatt gcgtggccga ctacagcgtg ctgtacaaca gcgccagctt     7800

cagcaccttc aagtgctacg gcgtgtcacc caccaagctg aacgacctgt gcttcaccaa     7860

cgtgtacgcc gacagcttcg tgatcagagg cgacgaagtg cggcagattg cccctggaca     7920

gacaggcaag atcgccgatt acaactacaa gctgcccgac gacttcaccg gctgtgtgat     7980

tgcctggaac agcaacaacc tggacagcaa agtcggcggc aactacaact acctgtaccg     8040

gctgttccgg aagtccaacc tgaagccttt cgagcgggac atcagcaccg agatctatca     8100

ggccggcagc accccttgca atggcgtgga aggcttcaac tgctacttcc cactgcagtc     8160

ctacggcttc cagcctacaa acggcgtggg ctaccagcct tacagagtgg tggtgctgag     8220

cttcgagctg ctgcatgctc ctgccacagt gtgcggccct aagaaaagca ccaacctggt     8280

caagaacaaa tgcgtgaact tcggcggagg cggaagtggt ggcggcggat ctaagtttct     8340

gaccaccgcc aaggacaaga acagatggga agatcccggc aagcagctgt acaatgtgga     8400

agccacaagc tacgcaggcg gcggaggaag cggaggcgga ggtagtaaat ttctgacaac     8460

ggctaaagat aagaatcgct gggaagatcc tgggaaacag ctctataacg tcgaggccac     8520

cagctatgct ggcggtggcg gatctggcgg cggtggttca aaattcctga ctacagccaa     8580

ggataagaat cgttgggaag atccaggcaa gcaactctat aatgttgagg ctacctctta     8640

cgctggtggc ggaggttctg gcggcggagg ctctaaattt ctcacaacag caaaggacaa     8700

gaatcgatgg gaagatccgg gaaaacaact gtacaacgtt gaggcaacat cctatgcagg     8760

cggaggtggc agtggcggag gtggaagcaa gtttctgact actgcaaaag ataagaatag     8820

atgggaagat cccgggaagc aactctacaa cgtcgaagct actagttatg ccggtggcgg     8880

tggatctggc ggaggcggca gcaaattcct gaccaccgct aaagacaaga atcgttggga     8940

agatcccgga aagcagttgt ataacgttga agctacgtcc tacgcctgag gcgcgcctat     9000

gttacgtgca aaggtgattg tcaccccccg aaagaccata ttgtgacaca ccctcagtat     9060

cacgcccaaa catttacagc cgcggtgtca aaaaccgcgt ggacgtggtt aacatccctg     9120

ctgggaggat cagccgtaat tattataatt ggcttggtgc tggctactat tgtggccatg     9180

tacgtgctga ccaaccagaa acataattga atacagcagc aattggcaag ctgcttacat     9240

agaactcgcg gcgattggca tgccgcctta aaatttttat tttatttttc ttttcttttc     9300

cgaatcggat tttgttttta atatttcaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     9360

aaaaaaatag gg                                                         9372


<210>  8
<211>  419
<212>  PRT
<213>  Severe acute respiratory syndrome-related coronavirus

<400>  8

Met Ser Asp Asn Gly Pro Gln Asn Gln Arg Asn Ala Pro Arg Ile Thr 
1               5                   10                  15      


Phe Gly Gly Pro Ser Asp Ser Thr Gly Ser Asn Gln Asn Gly Glu Arg 
            20                  25                  30          


Ser Gly Ala Arg Ser Lys Gln Arg Arg Pro Gln Gly Leu Pro Asn Asn 
        35                  40                  45              


Thr Ala Ser Trp Phe Thr Ala Leu Thr Gln His Gly Lys Glu Asp Leu 
    50                  55                  60                  


Lys Phe Pro Arg Gly Gln Gly Val Pro Ile Asn Thr Asn Ser Ser Pro 
65                  70                  75                  80  


Asp Asp Gln Ile Gly Tyr Tyr Arg Arg Ala Thr Arg Arg Ile Arg Gly 
                85                  90                  95      


Gly Asp Gly Lys Met Lys Asp Leu Ser Pro Arg Trp Tyr Phe Tyr Tyr 
            100                 105                 110         


Leu Gly Thr Gly Pro Glu Ala Gly Leu Pro Tyr Gly Ala Asn Lys Asp 
        115                 120                 125             


Gly Ile Ile Trp Val Ala Thr Glu Gly Ala Leu Asn Thr Pro Lys Asp 
    130                 135                 140                 


His Ile Gly Thr Arg Asn Pro Ala Asn Asn Ala Ala Ile Val Leu Gln 
145                 150                 155                 160 


Leu Pro Gln Gly Thr Thr Leu Pro Lys Gly Phe Tyr Ala Glu Gly Ser 
                165                 170                 175     


Arg Gly Gly Ser Gln Ala Ser Ser Arg Ser Ser Ser Arg Ser Arg Asn 
            180                 185                 190         


Ser Ser Arg Asn Ser Thr Pro Gly Ser Ser Arg Gly Thr Ser Pro Ala 
        195                 200                 205             


Arg Met Ala Gly Asn Gly Gly Asp Ala Ala Leu Ala Leu Leu Leu Leu 
    210                 215                 220                 


Asp Arg Leu Asn Gln Leu Glu Ser Lys Met Ser Gly Lys Gly Gln Gln 
225                 230                 235                 240 


Gln Gln Gly Gln Thr Val Thr Lys Lys Ser Ala Ala Glu Ala Ser Lys 
                245                 250                 255     


Lys Pro Arg Gln Lys Arg Thr Ala Thr Lys Ala Tyr Asn Val Thr Gln 
            260                 265                 270         


Ala Phe Gly Arg Arg Gly Pro Glu Gln Thr Gln Gly Asn Phe Gly Asp 
        275                 280                 285             


Gln Glu Leu Ile Arg Gln Gly Thr Asp Tyr Lys His Trp Pro Gln Ile 
    290                 295                 300                 


Ala Gln Phe Ala Pro Ser Ala Ser Ala Phe Phe Gly Met Ser Arg Ile 
305                 310                 315                 320 


Gly Met Glu Val Thr Pro Ser Gly Thr Trp Leu Thr Tyr Thr Gly Ala 
                325                 330                 335     


Ile Lys Leu Asp Asp Lys Asp Pro Asn Phe Lys Asp Gln Val Ile Leu 
            340                 345                 350         


Leu Asn Lys His Ile Asp Ala Tyr Lys Thr Phe Pro Pro Thr Glu Pro 
        355                 360                 365             


Lys Lys Asp Lys Lys Lys Lys Ala Asp Glu Thr Gln Ala Leu Pro Gln 
    370                 375                 380                 


Arg Gln Lys Lys Gln Gln Thr Val Thr Leu Leu Pro Ala Ala Asp Leu 
385                 390                 395                 400 


Asp Asp Phe Ser Lys Gln Leu Gln Gln Ser Met Ser Ser Ala Asp Ser 
                405                 410                 415     


Thr Gln Ala 
            


<210>  9
<211>  222
<212>  PRT
<213>  Severe acute respiratory syndrome-related coronavirus

<400>  9

Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      


Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          


Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
        35                  40                  45              


Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
    50                  55                  60                  


Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                  70                  75                  80  


Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 
                85                  90                  95      


Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
            100                 105                 110         


Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
        115                 120                 125             


Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 
    130                 135                 140                 


Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                 150                 155                 160 


Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 
                165                 170                 175     


Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
            180                 185                 190         


Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
        195                 200                 205             


Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    210                 215                 220         


<210>  10
<211>  7950
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of RNA replicon without antigen ORF

<400>  10
atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg       60

ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg      120

aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc      180

tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa      240

gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat      300

gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg      360

aaataactga taaggaattg gacaagaaaa tgaaggagct cgccgccgtc atgagcgacc      420

ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc      480

aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag      540

ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta      600

agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa      660

cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt      720

ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga      780

ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact      840

tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg      900

tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta      960

cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg     1020

tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac     1080

tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta     1140

tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg     1200

tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa     1260

ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc     1320

acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg     1380

atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa     1440

caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg     1500

acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt     1560

tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg     1620

tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa     1680

aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg     1740

ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga     1800

taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg     1860

tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca     1920

ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag     1980

gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg     2040

aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag     2100

ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa     2160

cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag     2220

gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga     2280

aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg     2340

ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata     2400

ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac     2460

ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc     2520

tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc     2580

gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa     2640

cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc     2700

aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca     2760

aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg     2820

ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg     2880

tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga     2940

taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag     3000

cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc     3060

agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca     3120

tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact     3180

cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg     3240

gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc     3300

cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc     3360

cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc     3420

gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag     3480

tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg     3540

gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt     3600

tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg     3660

tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc     3720

agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc     3780

tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa     3840

gcatcattgg tgctatagcg cggctgttca agttttcccg ggtatgcaaa ccgaaatcct     3900

cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc     3960

acaatcctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg     4020

aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag     4080

gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc     4140

tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac     4200

tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt     4260

cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca     4320

acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga     4380

acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg     4440

cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg     4500

ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg     4560

atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca     4620

caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg     4680

atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca     4740

tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg     4800

aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa     4860

gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat     4920

tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct     4980

caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag     5040

acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac     5100

cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg     5160

aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg     5220

aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat     5280

ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca     5340

gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc     5400

gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa     5460

gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc     5520

caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc     5580

ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga     5640

ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg     5700

catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa     5760

cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc     5820

tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta     5880

acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta     5940

ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc     6000

tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg     6060

cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta     6120

ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca     6180

ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac     6240

ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag     6300

ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg     6360

cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt     6420

ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa     6480

aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca     6540

taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa     6600

aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag     6660

cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga     6720

acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact     6780

tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg     6840

acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt     6900

tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta     6960

aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag     7020

tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg     7080

cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag     7140

acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga     7200

aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc     7260

gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg     7320

aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg     7380

gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca     7440

tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag     7500

gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa     7560

ggccaccggc gcgcctatgt tacgtgcaaa ggtgattgtc accccccgaa agaccatatt     7620

gtgacacacc ctcagtatca cgcccaaaca tttacagccg cggtgtcaaa aaccgcgtgg     7680

acgtggttaa catccctgct gggaggatca gccgtaatta ttataattgg cttggtgctg     7740

gctactattg tggccatgta cgtgctgacc aaccagaaac ataattgaat acagcagcaa     7800

ttggcaagct gcttacatag aactcgcggc gattggcatg ccgccttaaa atttttattt     7860

tatttttctt ttcttttccg aatcggattt tgtttttaat atttcaaaaa aaaaaaaaaa     7920

aaaaaaaaaa aaaaaaaaaa aaaaataggg                                      7950


<210>  11
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nonstructural protein 1

<400>  11

Met Glu Lys Val His Val Asp Ile Glu Glu Asp Ser Pro Phe Leu Arg 
1               5                   10                  15      


Ala Leu Gln Arg Ser Phe Pro Gln Phe Glu Val Glu Ala Lys Gln Val 
            20                  25                  30          


Thr Asp Asn Asp His Ala Asn Ala Arg Ala Phe Ser His Leu Ala Ser 
        35                  40                  45              


Lys Leu Ile Glu Thr Glu Val Asp Pro Ser Asp Thr Ile Leu Asp Ile 
    50                  55                  60                  


Gly Ser Ala Pro Ala Arg Arg Met Tyr Ser Lys His Lys Tyr His Cys 
65                  70                  75                  80  


Ile Cys Pro Met Arg Cys Ala Glu Asp Pro Asp Arg Leu Tyr Lys Tyr 
                85                  90                  95      


Ala Thr Lys Leu Lys Lys Asn Cys Lys Glu Ile Thr Asp Lys Glu Leu 
            100                 105                 110         


Asp Lys Lys Met Lys Glu Leu Ala Ala Val Met Ser Asp Pro Asp Leu 
        115                 120                 125             


Glu Thr Glu Thr Met Cys Leu His Asp Asp Glu Ser Cys Arg Tyr Glu 
    130                 135                 140                 


Gly Gln Val Ala Val Tyr Gln Asp Val Tyr Ala Val Asp Gly Pro Thr 
145                 150                 155                 160 


Ser Leu Tyr His Gln Ala Asn Lys Gly Val Arg Val Ala Tyr Trp Ile 
                165                 170                 175     


Gly Phe Asp Thr Thr Pro Phe Met Phe Lys Asn Leu Ala Gly Ala Tyr 
            180                 185                 190         


Pro Ser Tyr Ser Thr Asn Trp Ala Asp Glu Thr Val Leu Thr Ala Arg 
        195                 200                 205             


Asn Ile Gly Leu Cys Ser Ser Asp Val Met Glu Arg Ser Arg Arg Gly 
    210                 215                 220                 


Met Ser Ile Leu Arg Lys Lys Tyr Leu Lys Pro Ser Asn Asn Val Leu 
225                 230                 235                 240 


Phe Ser Val Gly Ser Thr Ile Tyr His Glu Lys Arg Asp Leu Leu Arg 
                245                 250                 255     


Ser Trp His Leu Pro Ser Val Phe His Leu Arg Gly Lys Gln Asn Tyr 
            260                 265                 270         


Thr Cys Arg Cys Glu Thr Ile Val Ser Cys Asp Gly Tyr Val Val Lys 
        275                 280                 285             


Arg Ile Ala Ile Ser Pro Gly Leu Tyr Gly Lys Pro Ser Gly Tyr Ala 
    290                 295                 300                 


Ala Thr Met His Arg Glu Gly Phe Leu Cys Cys Lys Val Thr Asp Thr 
305                 310                 315                 320 


Leu Asn Gly Glu Arg Val Ser Phe Pro Val Cys Thr Tyr Val Pro Ala 
                325                 330                 335     


Thr Leu Cys Asp Gln Met Thr Gly Ile Leu Ala Thr Asp Val Ser Ala 
            340                 345                 350         


Asp Asp Ala Gln Lys Leu Leu Val Gly Leu Asn Gln Arg Ile Val Val 
        355                 360                 365             


Asn Gly Arg Thr Gln Arg Asn Thr Asn Thr Met Lys Asn Tyr Leu Leu 
    370                 375                 380                 


Pro Val Val Ala Gln Ala Phe Ala Arg Trp Ala Lys Glu Tyr Lys Glu 
385                 390                 395                 400 


Asp Gln Glu Asp Glu Arg Pro Leu Gly Leu Arg Asp Arg Gln Leu Val 
                405                 410                 415     


Met Gly Cys Cys Trp Ala Phe Arg Arg His Lys Ile Thr Ser Ile Tyr 
            420                 425                 430         


Lys Arg Pro Asp Thr Gln Thr Ile Ile Lys Val Asn Ser Asp Phe His 
        435                 440                 445             


Ser Phe Val Leu Pro Arg Ile Gly Ser Asn Thr Leu Glu Ile Gly Leu 
    450                 455                 460                 


Arg Thr Arg Ile Arg Lys Met Leu Glu Glu His Lys Glu Pro Ser Pro 
465                 470                 475                 480 


Leu Ile Thr Ala Glu Asp Val Gln Glu Ala Lys Cys Ala Ala Asp Glu 
                485                 490                 495     


Ala Lys Glu Val Arg Glu Ala Glu Glu Leu Arg Ala Ala Leu Pro Pro 
            500                 505                 510         


Leu Ala Ala Asp Val Glu Glu Pro Thr Leu Glu Ala Asp Val Asp Leu 
        515                 520                 525             


Met Leu Gln Glu Ala Gly Ala 
    530                 535 


<210>  12
<211>  794
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nonstructural protein 2

<400>  12

Gly Ser Val Glu Thr Pro Arg Gly Leu Ile Lys Val Thr Ser Tyr Asp 
1               5                   10                  15      


Gly Glu Asp Lys Ile Gly Ser Tyr Ala Val Leu Ser Pro Gln Ala Val 
            20                  25                  30          


Leu Lys Ser Glu Lys Leu Ser Cys Ile His Pro Leu Ala Glu Gln Val 
        35                  40                  45              


Ile Val Ile Thr His Ser Gly Arg Lys Gly Arg Tyr Ala Val Glu Pro 
    50                  55                  60                  


Tyr His Gly Lys Val Val Val Pro Glu Gly His Ala Ile Pro Val Gln 
65                  70                  75                  80  


Asp Phe Gln Ala Leu Ser Glu Ser Ala Thr Ile Val Tyr Asn Glu Arg 
                85                  90                  95      


Glu Phe Val Asn Arg Tyr Leu His His Ile Ala Thr His Gly Gly Ala 
            100                 105                 110         


Leu Asn Thr Asp Glu Glu Tyr Tyr Lys Thr Val Lys Pro Ser Glu His 
        115                 120                 125             


Asp Gly Glu Tyr Leu Tyr Asp Ile Asp Arg Lys Gln Cys Val Lys Lys 
    130                 135                 140                 


Glu Leu Val Thr Gly Leu Gly Leu Thr Gly Glu Leu Val Asp Pro Pro 
145                 150                 155                 160 


Phe His Glu Phe Ala Tyr Glu Ser Leu Arg Thr Arg Pro Ala Ala Pro 
                165                 170                 175     


Tyr Gln Val Pro Thr Ile Gly Val Tyr Gly Val Pro Gly Ser Gly Lys 
            180                 185                 190         


Ser Gly Ile Ile Lys Ser Ala Val Thr Lys Lys Asp Leu Val Val Ser 
        195                 200                 205             


Ala Lys Lys Glu Asn Cys Ala Glu Ile Ile Arg Asp Val Lys Lys Met 
    210                 215                 220                 


Lys Gly Leu Asp Val Asn Ala Arg Thr Val Asp Ser Val Leu Leu Asn 
225                 230                 235                 240 


Gly Cys Lys His Pro Val Glu Thr Leu Tyr Ile Asp Glu Ala Phe Ala 
                245                 250                 255     


Cys His Ala Gly Thr Leu Arg Ala Leu Ile Ala Ile Ile Arg Pro Lys 
            260                 265                 270         


Lys Ala Val Leu Cys Gly Asp Pro Lys Gln Cys Gly Phe Phe Asn Met 
        275                 280                 285             


Met Cys Leu Lys Val His Phe Asn His Glu Ile Cys Thr Gln Val Phe 
    290                 295                 300                 


His Lys Ser Ile Ser Arg Arg Cys Thr Lys Ser Val Thr Ser Val Val 
305                 310                 315                 320 


Ser Thr Leu Phe Tyr Asp Lys Lys Met Arg Thr Thr Asn Pro Lys Glu 
                325                 330                 335     


Thr Lys Ile Val Ile Asp Thr Thr Gly Ser Thr Lys Pro Lys Gln Asp 
            340                 345                 350         


Asp Leu Ile Leu Thr Cys Phe Arg Gly Trp Val Lys Gln Leu Gln Ile 
        355                 360                 365             


Asp Tyr Lys Gly Asn Glu Ile Met Thr Ala Ala Ala Ser Gln Gly Leu 
    370                 375                 380                 


Thr Arg Lys Gly Val Tyr Ala Val Arg Tyr Lys Val Asn Glu Asn Pro 
385                 390                 395                 400 


Leu Tyr Ala Pro Thr Ser Glu His Val Asn Val Leu Leu Thr Arg Thr 
                405                 410                 415     


Glu Asp Arg Ile Val Trp Lys Thr Leu Ala Gly Asp Pro Trp Ile Lys 
            420                 425                 430         


Thr Leu Thr Ala Lys Tyr Pro Gly Asn Phe Thr Ala Thr Ile Glu Glu 
        435                 440                 445             


Trp Gln Ala Glu His Asp Ala Ile Met Arg His Ile Leu Glu Arg Pro 
    450                 455                 460                 


Asp Pro Thr Asp Val Phe Gln Asn Lys Ala Asn Val Cys Trp Ala Lys 
465                 470                 475                 480 


Ala Leu Val Pro Val Leu Lys Thr Ala Gly Ile Asp Met Thr Thr Glu 
                485                 490                 495     


Gln Trp Asn Thr Val Asp Tyr Phe Glu Thr Asp Lys Ala His Ser Ala 
            500                 505                 510         


Glu Ile Val Leu Asn Gln Leu Cys Val Arg Phe Phe Gly Leu Asp Leu 
        515                 520                 525             


Asp Ser Gly Leu Phe Ser Ala Pro Thr Val Pro Leu Ser Ile Arg Asn 
    530                 535                 540                 


Asn His Trp Asp Asn Ser Pro Ser Pro Asn Met Tyr Gly Leu Asn Lys 
545                 550                 555                 560 


Glu Val Val Arg Gln Leu Ser Arg Arg Tyr Pro Gln Leu Pro Arg Ala 
                565                 570                 575     


Val Ala Thr Gly Arg Val Tyr Asp Met Asn Thr Gly Thr Leu Arg Asn 
            580                 585                 590         


Tyr Asp Pro Arg Ile Asn Leu Val Pro Val Asn Arg Arg Leu Pro His 
        595                 600                 605             


Ala Leu Val Leu His His Asn Glu His Pro Gln Ser Asp Phe Ser Ser 
    610                 615                 620                 


Phe Val Ser Lys Leu Lys Gly Arg Thr Val Leu Val Val Gly Glu Lys 
625                 630                 635                 640 


Leu Ser Val Pro Gly Lys Met Val Asp Trp Leu Ser Asp Arg Pro Glu 
                645                 650                 655     


Ala Thr Phe Arg Ala Arg Leu Asp Leu Gly Ile Pro Gly Asp Val Pro 
            660                 665                 670         


Lys Tyr Asp Ile Ile Phe Val Asn Val Arg Thr Pro Tyr Lys Tyr His 
        675                 680                 685             


His Tyr Gln Gln Cys Glu Asp His Ala Ile Lys Leu Ser Met Leu Thr 
    690                 695                 700                 


Lys Lys Ala Cys Leu His Leu Asn Pro Gly Gly Thr Cys Val Ser Ile 
705                 710                 715                 720 


Gly Tyr Gly Tyr Ala Asp Arg Ala Ser Glu Ser Ile Ile Gly Ala Ile 
                725                 730                 735     


Ala Arg Gln Phe Lys Phe Ser Arg Val Cys Lys Pro Lys Ser Ser Leu 
            740                 745                 750         


Glu Glu Thr Glu Val Leu Phe Val Phe Ile Gly Tyr Asp Arg Lys Ala 
        755                 760                 765             


Arg Thr His Asn Pro Tyr Lys Leu Ser Ser Thr Leu Thr Asn Ile Tyr 
    770                 775                 780                 


Thr Gly Ser Arg Leu His Glu Ala Gly Cys 
785                 790                 


<210>  13
<211>  550
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nonstructural protein 3

<400>  13

Ala Pro Ser Tyr His Val Val Arg Gly Asp Ile Ala Thr Ala Thr Glu 
1               5                   10                  15      


Gly Val Ile Ile Asn Ala Ala Asn Ser Lys Gly Gln Pro Gly Gly Gly 
            20                  25                  30          


Val Cys Gly Ala Leu Tyr Lys Lys Phe Pro Glu Ser Phe Asp Leu Gln 
        35                  40                  45              


Pro Ile Glu Val Gly Lys Ala Arg Leu Val Lys Gly Ala Ala Lys His 
    50                  55                  60                  


Ile Ile His Ala Val Gly Pro Asn Phe Asn Lys Val Ser Glu Val Glu 
65                  70                  75                  80  


Gly Asp Lys Gln Leu Ala Glu Ala Tyr Glu Ser Ile Ala Lys Ile Val 
                85                  90                  95      


Asn Asp Asn Asn Tyr Lys Ser Val Ala Ile Pro Leu Leu Ser Thr Gly 
            100                 105                 110         


Ile Phe Ser Gly Asn Lys Asp Arg Leu Thr Gln Ser Leu Asn His Leu 
        115                 120                 125             


Leu Thr Ala Leu Asp Thr Thr Asp Ala Asp Val Ala Ile Tyr Cys Arg 
    130                 135                 140                 


Asp Lys Lys Trp Glu Met Thr Leu Lys Glu Ala Val Ala Arg Arg Glu 
145                 150                 155                 160 


Ala Val Glu Glu Ile Cys Ile Ser Asp Asp Ser Ser Val Thr Glu Pro 
                165                 170                 175     


Asp Ala Glu Leu Val Arg Val His Pro Lys Ser Ser Leu Ala Gly Arg 
            180                 185                 190         


Lys Gly Tyr Ser Thr Ser Asp Gly Lys Thr Phe Ser Tyr Leu Glu Gly 
        195                 200                 205             


Thr Lys Phe His Gln Ala Ala Lys Asp Ile Ala Glu Ile Asn Ala Met 
    210                 215                 220                 


Trp Pro Val Ala Thr Glu Ala Asn Glu Gln Val Cys Met Tyr Ile Leu 
225                 230                 235                 240 


Gly Glu Ser Met Ser Ser Ile Arg Ser Lys Cys Pro Val Glu Glu Ser 
                245                 250                 255     


Glu Ala Ser Thr Pro Pro Ser Thr Leu Pro Cys Leu Cys Ile His Ala 
            260                 265                 270         


Met Thr Pro Glu Arg Val Gln Arg Leu Lys Ala Ser Arg Pro Glu Gln 
        275                 280                 285             


Ile Thr Val Cys Ser Ser Phe Pro Leu Pro Lys Tyr Arg Ile Thr Gly 
    290                 295                 300                 


Val Gln Lys Ile Gln Cys Ser Gln Pro Ile Leu Phe Ser Pro Lys Val 
305                 310                 315                 320 


Pro Ala Tyr Ile His Pro Arg Lys Tyr Leu Val Glu Thr Pro Pro Val 
                325                 330                 335     


Asp Glu Thr Pro Glu Pro Ser Ala Glu Asn Gln Ser Thr Glu Gly Thr 
            340                 345                 350         


Pro Glu Gln Pro Pro Leu Ile Thr Glu Asp Glu Thr Arg Thr Arg Thr 
        355                 360                 365             


Pro Glu Pro Ile Ile Ile Glu Glu Glu Glu Glu Asp Ser Ile Ser Leu 
    370                 375                 380                 


Leu Ser Asp Gly Pro Thr His Gln Val Leu Gln Val Glu Ala Asp Ile 
385                 390                 395                 400 


His Gly Pro Pro Ser Val Ser Ser Ser Ser Trp Ser Ile Pro His Ala 
                405                 410                 415     


Ser Asp Phe Asp Val Asp Ser Leu Ser Ile Leu Asp Thr Leu Glu Gly 
            420                 425                 430         


Ala Ser Val Thr Ser Gly Ala Thr Ser Ala Glu Thr Asn Ser Tyr Phe 
        435                 440                 445             


Ala Lys Ser Met Glu Phe Leu Ala Arg Pro Val Pro Ala Pro Arg Thr 
    450                 455                 460                 


Val Phe Arg Asn Pro Pro His Pro Ala Pro Arg Thr Arg Thr Pro Ser 
465                 470                 475                 480 


Leu Ala Pro Ser Arg Ala Cys Ser Arg Thr Ser Leu Val Ser Thr Pro 
                485                 490                 495     


Pro Gly Val Asn Arg Val Ile Thr Arg Glu Glu Leu Glu Ala Leu Thr 
            500                 505                 510         


Pro Ser Arg Thr Pro Ser Arg Ser Val Ser Arg Thr Ser Leu Val Ser 
        515                 520                 525             


Asn Pro Pro Gly Val Asn Arg Val Ile Thr Arg Glu Glu Phe Glu Ala 
    530                 535                 540                 


Phe Val Ala Gln Gln Gln 
545                 550 


<210>  14
<211>  607
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nonstructural protein 4

<400>  14

Tyr Ile Phe Ser Ser Asp Thr Gly Gln Gly His Leu Gln Gln Lys Ser 
1               5                   10                  15      


Val Arg Gln Thr Val Leu Ser Glu Val Val Leu Glu Arg Thr Glu Leu 
            20                  25                  30          


Glu Ile Ser Tyr Ala Pro Arg Leu Asp Gln Glu Lys Glu Glu Leu Leu 
        35                  40                  45              


Arg Lys Lys Leu Gln Leu Asn Pro Thr Pro Ala Asn Arg Ser Arg Tyr 
    50                  55                  60                  


Gln Ser Arg Lys Val Glu Asn Met Lys Ala Ile Thr Ala Arg Arg Ile 
65                  70                  75                  80  


Leu Gln Gly Leu Gly His Tyr Leu Lys Ala Glu Gly Lys Val Glu Cys 
                85                  90                  95      


Tyr Arg Thr Leu His Pro Val Pro Leu Tyr Ser Ser Ser Val Asn Arg 
            100                 105                 110         


Ala Phe Ser Ser Pro Lys Val Ala Val Glu Ala Cys Asn Ala Met Leu 
        115                 120                 125             


Lys Glu Asn Phe Pro Thr Val Ala Ser Tyr Cys Ile Ile Pro Glu Tyr 
    130                 135                 140                 


Asp Ala Tyr Leu Asp Met Val Asp Gly Ala Ser Cys Cys Leu Asp Thr 
145                 150                 155                 160 


Ala Ser Phe Cys Pro Ala Lys Leu Arg Ser Phe Pro Lys Lys His Ser 
                165                 170                 175     


Tyr Leu Glu Pro Thr Ile Arg Ser Ala Val Pro Ser Ala Ile Gln Asn 
            180                 185                 190         


Thr Leu Gln Asn Val Leu Ala Ala Ala Thr Lys Arg Asn Cys Asn Val 
        195                 200                 205             


Thr Gln Met Arg Glu Leu Pro Val Leu Asp Ser Ala Ala Phe Asn Val 
    210                 215                 220                 


Glu Cys Phe Lys Lys Tyr Ala Cys Asn Asn Glu Tyr Trp Glu Thr Phe 
225                 230                 235                 240 


Lys Glu Asn Pro Ile Arg Leu Thr Glu Glu Asn Val Val Asn Tyr Ile 
                245                 250                 255     


Thr Lys Leu Lys Gly Pro Lys Ala Ala Ala Leu Phe Ala Lys Thr His 
            260                 265                 270         


Asn Leu Asn Met Leu Gln Asp Ile Pro Met Asp Arg Phe Val Met Asp 
        275                 280                 285             


Leu Lys Arg Asp Val Lys Val Thr Pro Gly Thr Lys His Thr Glu Glu 
    290                 295                 300                 


Arg Pro Lys Val Gln Val Ile Gln Ala Ala Asp Pro Leu Ala Thr Ala 
305                 310                 315                 320 


Tyr Leu Cys Gly Ile His Arg Glu Leu Val Arg Arg Leu Asn Ala Val 
                325                 330                 335     


Leu Leu Pro Asn Ile His Thr Leu Phe Asp Met Ser Ala Glu Asp Phe 
            340                 345                 350         


Asp Ala Ile Ile Ala Glu His Phe Gln Pro Gly Asp Cys Val Leu Glu 
        355                 360                 365             


Thr Asp Ile Ala Ser Phe Asp Lys Ser Glu Asp Asp Ala Met Ala Leu 
    370                 375                 380                 


Thr Ala Leu Met Ile Leu Glu Asp Leu Gly Val Asp Ala Glu Leu Leu 
385                 390                 395                 400 


Thr Leu Ile Glu Ala Ala Phe Gly Glu Ile Ser Ser Ile His Leu Pro 
                405                 410                 415     


Thr Lys Thr Lys Phe Lys Phe Gly Ala Met Met Lys Ser Gly Met Phe 
            420                 425                 430         


Leu Thr Leu Phe Val Asn Thr Val Ile Asn Ile Val Ile Ala Ser Arg 
        435                 440                 445             


Val Leu Arg Glu Arg Leu Thr Gly Ser Pro Cys Ala Ala Phe Ile Gly 
    450                 455                 460                 


Asp Asp Asn Ile Val Lys Gly Val Lys Ser Asp Lys Leu Met Ala Asp 
465                 470                 475                 480 


Arg Cys Ala Thr Trp Leu Asn Met Glu Val Lys Ile Ile Asp Ala Val 
                485                 490                 495     


Val Gly Glu Lys Ala Pro Tyr Phe Cys Gly Gly Phe Ile Leu Cys Asp 
            500                 505                 510         


Ser Val Thr Gly Thr Ala Cys Arg Val Ala Asp Pro Leu Lys Arg Leu 
        515                 520                 525             


Phe Lys Leu Gly Lys Pro Leu Ala Ala Asp Asp Glu His Asp Asp Asp 
    530                 535                 540                 


Arg Arg Arg Ala Leu His Glu Glu Ser Thr Arg Trp Asn Arg Val Gly 
545                 550                 555                 560 


Ile Leu Ser Glu Leu Cys Lys Ala Val Glu Ser Arg Tyr Glu Thr Val 
                565                 570                 575     


Gly Thr Ser Ile Ile Val Met Ala Met Thr Thr Leu Ala Ser Ser Val 
            580                 585                 590         


Lys Ser Phe Ser Tyr Leu Arg Gly Ala Pro Ile Thr Leu Tyr Gly 
        595                 600                 605         


<210>  15
<211>  794
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nonstructural protein 2 with Q739L mutation

<400>  15

Gly Ser Val Glu Thr Pro Arg Gly Leu Ile Lys Val Thr Ser Tyr Asp 
1               5                   10                  15      


Gly Glu Asp Lys Ile Gly Ser Tyr Ala Val Leu Ser Pro Gln Ala Val 
            20                  25                  30          


Leu Lys Ser Glu Lys Leu Ser Cys Ile His Pro Leu Ala Glu Gln Val 
        35                  40                  45              


Ile Val Ile Thr His Ser Gly Arg Lys Gly Arg Tyr Ala Val Glu Pro 
    50                  55                  60                  


Tyr His Gly Lys Val Val Val Pro Glu Gly His Ala Ile Pro Val Gln 
65                  70                  75                  80  


Asp Phe Gln Ala Leu Ser Glu Ser Ala Thr Ile Val Tyr Asn Glu Arg 
                85                  90                  95      


Glu Phe Val Asn Arg Tyr Leu His His Ile Ala Thr His Gly Gly Ala 
            100                 105                 110         


Leu Asn Thr Asp Glu Glu Tyr Tyr Lys Thr Val Lys Pro Ser Glu His 
        115                 120                 125             


Asp Gly Glu Tyr Leu Tyr Asp Ile Asp Arg Lys Gln Cys Val Lys Lys 
    130                 135                 140                 


Glu Leu Val Thr Gly Leu Gly Leu Thr Gly Glu Leu Val Asp Pro Pro 
145                 150                 155                 160 


Phe His Glu Phe Ala Tyr Glu Ser Leu Arg Thr Arg Pro Ala Ala Pro 
                165                 170                 175     


Tyr Gln Val Pro Thr Ile Gly Val Tyr Gly Val Pro Gly Ser Gly Lys 
            180                 185                 190         


Ser Gly Ile Ile Lys Ser Ala Val Thr Lys Lys Asp Leu Val Val Ser 
        195                 200                 205             


Ala Lys Lys Glu Asn Cys Ala Glu Ile Ile Arg Asp Val Lys Lys Met 
    210                 215                 220                 


Lys Gly Leu Asp Val Asn Ala Arg Thr Val Asp Ser Val Leu Leu Asn 
225                 230                 235                 240 


Gly Cys Lys His Pro Val Glu Thr Leu Tyr Ile Asp Glu Ala Phe Ala 
                245                 250                 255     


Cys His Ala Gly Thr Leu Arg Ala Leu Ile Ala Ile Ile Arg Pro Lys 
            260                 265                 270         


Lys Ala Val Leu Cys Gly Asp Pro Lys Gln Cys Gly Phe Phe Asn Met 
        275                 280                 285             


Met Cys Leu Lys Val His Phe Asn His Glu Ile Cys Thr Gln Val Phe 
    290                 295                 300                 


His Lys Ser Ile Ser Arg Arg Cys Thr Lys Ser Val Thr Ser Val Val 
305                 310                 315                 320 


Ser Thr Leu Phe Tyr Asp Lys Lys Met Arg Thr Thr Asn Pro Lys Glu 
                325                 330                 335     


Thr Lys Ile Val Ile Asp Thr Thr Gly Ser Thr Lys Pro Lys Gln Asp 
            340                 345                 350         


Asp Leu Ile Leu Thr Cys Phe Arg Gly Trp Val Lys Gln Leu Gln Ile 
        355                 360                 365             


Asp Tyr Lys Gly Asn Glu Ile Met Thr Ala Ala Ala Ser Gln Gly Leu 
    370                 375                 380                 


Thr Arg Lys Gly Val Tyr Ala Val Arg Tyr Lys Val Asn Glu Asn Pro 
385                 390                 395                 400 


Leu Tyr Ala Pro Thr Ser Glu His Val Asn Val Leu Leu Thr Arg Thr 
                405                 410                 415     


Glu Asp Arg Ile Val Trp Lys Thr Leu Ala Gly Asp Pro Trp Ile Lys 
            420                 425                 430         


Thr Leu Thr Ala Lys Tyr Pro Gly Asn Phe Thr Ala Thr Ile Glu Glu 
        435                 440                 445             


Trp Gln Ala Glu His Asp Ala Ile Met Arg His Ile Leu Glu Arg Pro 
    450                 455                 460                 


Asp Pro Thr Asp Val Phe Gln Asn Lys Ala Asn Val Cys Trp Ala Lys 
465                 470                 475                 480 


Ala Leu Val Pro Val Leu Lys Thr Ala Gly Ile Asp Met Thr Thr Glu 
                485                 490                 495     


Gln Trp Asn Thr Val Asp Tyr Phe Glu Thr Asp Lys Ala His Ser Ala 
            500                 505                 510         


Glu Ile Val Leu Asn Gln Leu Cys Val Arg Phe Phe Gly Leu Asp Leu 
        515                 520                 525             


Asp Ser Gly Leu Phe Ser Ala Pro Thr Val Pro Leu Ser Ile Arg Asn 
    530                 535                 540                 


Asn His Trp Asp Asn Ser Pro Ser Pro Asn Met Tyr Gly Leu Asn Lys 
545                 550                 555                 560 


Glu Val Val Arg Gln Leu Ser Arg Arg Tyr Pro Gln Leu Pro Arg Ala 
                565                 570                 575     


Val Ala Thr Gly Arg Val Tyr Asp Met Asn Thr Gly Thr Leu Arg Asn 
            580                 585                 590         


Tyr Asp Pro Arg Ile Asn Leu Val Pro Val Asn Arg Arg Leu Pro His 
        595                 600                 605             


Ala Leu Val Leu His His Asn Glu His Pro Gln Ser Asp Phe Ser Ser 
    610                 615                 620                 


Phe Val Ser Lys Leu Lys Gly Arg Thr Val Leu Val Val Gly Glu Lys 
625                 630                 635                 640 


Leu Ser Val Pro Gly Lys Met Val Asp Trp Leu Ser Asp Arg Pro Glu 
                645                 650                 655     


Ala Thr Phe Arg Ala Arg Leu Asp Leu Gly Ile Pro Gly Asp Val Pro 
            660                 665                 670         


Lys Tyr Asp Ile Ile Phe Val Asn Val Arg Thr Pro Tyr Lys Tyr His 
        675                 680                 685             


His Tyr Gln Gln Cys Glu Asp His Ala Ile Lys Leu Ser Met Leu Thr 
    690                 695                 700                 


Lys Lys Ala Cys Leu His Leu Asn Pro Gly Gly Thr Cys Val Ser Ile 
705                 710                 715                 720 


Gly Tyr Gly Tyr Ala Asp Arg Ala Ser Glu Ser Ile Ile Gly Ala Ile 
                725                 730                 735     


Ala Arg Leu Phe Lys Phe Ser Arg Val Cys Lys Pro Lys Ser Ser Leu 
            740                 745                 750         


Glu Glu Thr Glu Val Leu Phe Val Phe Ile Gly Tyr Asp Arg Lys Ala 
        755                 760                 765             


Arg Thr His Asn Pro Tyr Lys Leu Ser Ser Thr Leu Thr Asn Ile Tyr 
    770                 775                 780                 


Thr Gly Ser Arg Leu His Glu Ala Gly Cys 
785                 790                 


<210>  16
<211>  1273
<212>  PRT
<213>  Severe acute respiratory syndrome-related coronavirus

<400>  16

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  17
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA equivalent of SARS-CoV-2 Spike protein

<400>  17
atgttcgtgt tcctggtgct gctgcctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctaccccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg tccggcacca atggcaccaa gagattcgac      240

aaccccgtgc tgcccttcaa cgacggggtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgaccccttc      420

ctgggcgtct actaccacaa gaacaacaag agctggatgg aaagcgagtt ccgggtgtac      480

agcagcgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaacttcaa gaacctgcgc gagttcgtgt ttaagaacat cgacggctac      600

ttcaagatct acagcaagca cacccctatc aacctcgtgc gggatctgcc tcagggcttc      660

tctgctctgg aacccctggt ggatctgccc atcggcatca acatcacccg gtttcagaca      720

ctgctggccc tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgccgccg cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga cgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggaaaag ggcatctacc agaccagcaa cttccgggtg      960

cagcccaccg aatccatcgt gcggttcccc aatatcacca atctgtgccc cttcggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgtggccg actactccgt gctgtacaac tccgccagct tcagcacctt caagtgctac     1140

ggcgtgtccc ctaccaagct gaacgacctg tgcttcacaa acgtgtacgc cgacagcttc     1200

gtgatccggg gagatgaagt gcggcagatt gcccctggac agacaggcaa gatcgccgac     1260

tacaactaca agctgcccga cgacttcacc ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggcgg caactacaat tacctgtacc ggctgttccg gaagtccaat     1380

ctgaagccct tcgagcggga catctccacc gagatctatc aggccggcag caccccttgt     1440

aacggcgtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgcc     1560

cctgccacag tgtgcggccc taagaaaagc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggcctgac cggcaccggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggccg ggatatcgcc gataccacag acgccgttag agatccccag     1740

acactggaaa tcctggacat caccccttgc agcttcggcg gagtgtctgt gatcacccct     1800

ggcaccaaca ccagcaatca ggtggcagtg ctgtaccagg acgtgaactg taccgaagtg     1860

cccgtggcca ttcacgccga tcagctgaca cctacatggc gggtgtactc caccggcagc     1920

aatgtgtttc agaccagagc cggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccccatcgg cgctggcatc tgtgccagct accagacaca gacaaacagc     2040

cccagacggg ccagatctgt ggccagccag agcatcattg cctacacaat gtctctgggc     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccccaccaa cttcaccatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgcg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gccgtggaac aggacaagaa cacccaagag     2340

gtgttcgccc aagtgaagca gatctacaag acccctccta tcaaggactt cggcggcttc     2400

aatttcagcc agattctgcc cgatcctagc aagcccagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac actggccgac gccggcttca tcaagcagta tggcgattgt     2520

ctgggcgaca ttgccgccag ggatctgatt tgcgcccaga agtttaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gcccagtaca catctgccct gctggccggc     2640

acaatcacaa gcggctggac atttggagct ggcgccgctc tgcagatccc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caacagcgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg ccctgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggcgccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggacaaggt ggaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagccgccga gattagagcc     3060

tctgccaatc tggccgccac caagatgtct gagtgtgtgc tgggccagag caagagagtg     3120

gacttttgcg gcaagggcta ccacctgatg agcttccctc agtctgcccc tcacggcgtg     3180

gtgtttctgc acgtgacata tgtgcccgct caagagaaga atttcaccac cgctccagcc     3240

atctgccacg acggcaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagccccaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtga acaataccgt gtacgaccct     3420

ctgcagcccg agctggacag cttcaaagag gaactggaca agtactttaa gaaccacaca     3480

agccccgacg tggacctggg cgatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaactgg ggaagtacga gcagtacatc aagtggccct ggtacatctg gctgggcttt     3660

atcgccggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgcc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagcccg tgctgaaggg cgtgaaactg cactacacct ga                        3822


<210>  18
<211>  556
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  nonstructural protein 4 variant after readthrough

<400>  18

Ala Pro Ser Tyr His Val Val Arg Gly Asp Ile Ala Thr Ala Thr Glu 
1               5                   10                  15      


Gly Val Ile Ile Asn Ala Ala Asn Ser Lys Gly Gln Pro Gly Gly Gly 
            20                  25                  30          


Val Cys Gly Ala Leu Tyr Lys Lys Phe Pro Glu Ser Phe Asp Leu Gln 
        35                  40                  45              


Pro Ile Glu Val Gly Lys Ala Arg Leu Val Lys Gly Ala Ala Lys His 
    50                  55                  60                  


Ile Ile His Ala Val Gly Pro Asn Phe Asn Lys Val Ser Glu Val Glu 
65                  70                  75                  80  


Gly Asp Lys Gln Leu Ala Glu Ala Tyr Glu Ser Ile Ala Lys Ile Val 
                85                  90                  95      


Asn Asp Asn Asn Tyr Lys Ser Val Ala Ile Pro Leu Leu Ser Thr Gly 
            100                 105                 110         


Ile Phe Ser Gly Asn Lys Asp Arg Leu Thr Gln Ser Leu Asn His Leu 
        115                 120                 125             


Leu Thr Ala Leu Asp Thr Thr Asp Ala Asp Val Ala Ile Tyr Cys Arg 
    130                 135                 140                 


Asp Lys Lys Trp Glu Met Thr Leu Lys Glu Ala Val Ala Arg Arg Glu 
145                 150                 155                 160 


Ala Val Glu Glu Ile Cys Ile Ser Asp Asp Ser Ser Val Thr Glu Pro 
                165                 170                 175     


Asp Ala Glu Leu Val Arg Val His Pro Lys Ser Ser Leu Ala Gly Arg 
            180                 185                 190         


Lys Gly Tyr Ser Thr Ser Asp Gly Lys Thr Phe Ser Tyr Leu Glu Gly 
        195                 200                 205             


Thr Lys Phe His Gln Ala Ala Lys Asp Ile Ala Glu Ile Asn Ala Met 
    210                 215                 220                 


Trp Pro Val Ala Thr Glu Ala Asn Glu Gln Val Cys Met Tyr Ile Leu 
225                 230                 235                 240 


Gly Glu Ser Met Ser Ser Ile Arg Ser Lys Cys Pro Val Glu Glu Ser 
                245                 250                 255     


Glu Ala Ser Thr Pro Pro Ser Thr Leu Pro Cys Leu Cys Ile His Ala 
            260                 265                 270         


Met Thr Pro Glu Arg Val Gln Arg Leu Lys Ala Ser Arg Pro Glu Gln 
        275                 280                 285             


Ile Thr Val Cys Ser Ser Phe Pro Leu Pro Lys Tyr Arg Ile Thr Gly 
    290                 295                 300                 


Val Gln Lys Ile Gln Cys Ser Gln Pro Ile Leu Phe Ser Pro Lys Val 
305                 310                 315                 320 


Pro Ala Tyr Ile His Pro Arg Lys Tyr Leu Val Glu Thr Pro Pro Val 
                325                 330                 335     


Asp Glu Thr Pro Glu Pro Ser Ala Glu Asn Gln Ser Thr Glu Gly Thr 
            340                 345                 350         


Pro Glu Gln Pro Pro Leu Ile Thr Glu Asp Glu Thr Arg Thr Arg Thr 
        355                 360                 365             


Pro Glu Pro Ile Ile Ile Glu Glu Glu Glu Glu Asp Ser Ile Ser Leu 
    370                 375                 380                 


Leu Ser Asp Gly Pro Thr His Gln Val Leu Gln Val Glu Ala Asp Ile 
385                 390                 395                 400 


His Gly Pro Pro Ser Val Ser Ser Ser Ser Trp Ser Ile Pro His Ala 
                405                 410                 415     


Ser Asp Phe Asp Val Asp Ser Leu Ser Ile Leu Asp Thr Leu Glu Gly 
            420                 425                 430         


Ala Ser Val Thr Ser Gly Ala Thr Ser Ala Glu Thr Asn Ser Tyr Phe 
        435                 440                 445             


Ala Lys Ser Met Glu Phe Leu Ala Arg Pro Val Pro Ala Pro Arg Thr 
    450                 455                 460                 


Val Phe Arg Asn Pro Pro His Pro Ala Pro Arg Thr Arg Thr Pro Ser 
465                 470                 475                 480 


Leu Ala Pro Ser Arg Ala Cys Ser Arg Thr Ser Leu Val Ser Thr Pro 
                485                 490                 495     


Pro Gly Val Asn Arg Val Ile Thr Arg Glu Glu Leu Glu Ala Leu Thr 
            500                 505                 510         


Pro Ser Arg Thr Pro Ser Arg Ser Val Ser Arg Thr Ser Leu Val Ser 
        515                 520                 525             


Asn Pro Pro Gly Val Asn Arg Val Ile Thr Arg Glu Glu Phe Glu Ala 
    530                 535                 540                 


Phe Val Ala Gln Gln Gln Arg Phe Asp Ala Gly Ala 
545                 550                 555   

  
<210>  19
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human tissue plasminogen activator leader peptide

<400>  19

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro 
            20                            




