               SEQUENCE LISTING

<110> Intervet International B.V.

<120> Swine Influenza A Virus Vaccine

<130> 24972 EPPD

<160> 25

<170> BiSSAP 1.3.6

<210> 1
<211> 1695
<212> DNA
<213> Influenza A virus


<220> 
<223> >KU323133.1 Influenza A virus (A/swine/Italy/3033-1/2015(H1N2))
      segment 4 hemagglutinin (HA) gene, complete cds

<400> 1
atgaaagcta aactactaat cctgttgtgt gcactttcaa ctacagatgc agacacaata      60

tgtataggat accatgctaa caattcaacc gacaccgttg acacagtcct agaaaagaat     120

gtgacagtaa cacactctgt caacctcctt gaggacagtc acaatgggaa actatgtaaa     180

ctgaagggag tggccccact gcaattggga aaatgcagca tcgcaggatg gatactaggg     240

aacccagagt gtgaatcact gctttctaag aaatcatggt cctatattgc agaagcacca     300

aatgctgaga atggaatatg ttacccaggg cacttctccg actacgaaga gctgagggag     360

caattaagtt cagtatcttc attcgagagg ttcgaaatat ttcctaagga gagttcatgg     420

cccaaacaca gcataggagc aactgcatca tgctccaaac aagggaaaag cagtttttat     480

acaaatctgc tatggctaac tgaaaaaaat gggtcttatc caaatttgac catgtcctat     540

atgaacgaca aagagagaga agtccttgtg ctatggggag tccatcatcc gtccaacata     600

gaggaccaaa gagcaatcta tcgcaaagaa actgcttatg tttctgtaat gtcatcacac     660

tacaacaaga ggttcacccc agagattgca aaaagaccca aagtaaggaa tcaagagggg     720

agaatcaact actactggac actgctggaa cccagggaca caataatatt tgaagcaaat     780

gggaatttga tagcaccatg gtatgctttt gcattgaata gaggctttga gtcaggaatc     840

attgtctcaa atgcatcgat ggatgagtgt aacgcgaagt gtcaaacgcc ccgaggagcg     900

ataaacaata gcctgccttt ccagaatgta cacccaatca caatagggga atgccccaag     960

tatgtaaaga gtacaaaatt aaagatggct acaggactta gaaacattcc atccatccaa    1020

tccagaggtt tgtttggagc cattgctggt ttcattgaag ggggatggac cggaatgata    1080

gatggatggt atggttatca tcatcagaat gaacagggtt ctggctatgc tgcagatcaa    1140

aaaagcacac aaaatgccat taatgggatt acaaacaagg tgaactctgt tatcgataaa    1200

atgaacactc aatttacagc tgtaggtaaa gaattcaaca gattagaaaa aagaatggaa    1260

aacctgaata agaaggttga tgacggtttt cttgacattt ggacgtataa tgcagaattg    1320

ttggttctgc tggagaatga gaggactttg gattttcatg attcaaatgt gaagagctta    1380

tatgaaggag taaaggccca attgaagaat aatgccaaag aagtaggaaa cgggtgtttt    1440

gaattctacc acaagtgtga caacgaatgc atggacagtg taaaaaatgg aacttatgac    1500

tatccaagat actcagagga atcaaaatta aacagagaac aaattgatgg agtggaattg    1560

aaatcaatgg gagtctatca gatcctggcg atatactcaa ctgccgccag ttcattggtg    1620

cttttagtca ccctgggggc aatcagtttt tggatgtgtt ctaatggttc tttgcagtgc    1680

agaatatgta tctga                                                     1695


<210> 2
<211> 1695
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUH1-17 Swine optimized sequence

<400> 2
atgaaagcca agctcctcat tctcctttgt gccctgtcca cgaccgacgc tgatactatc      60

tgcatcggct accatgccaa caactccact gataccgtgg acaccgtgct ggaaaagaac     120

gtgaccgtga ctcactcggt gaacctcctt gaggacagcc acaacgggaa gctctgcaag     180

ctgaagggcg tggcacccct acaactggga aagtgttcca tcgcgggctg gattctggga     240

aaccccgagt gcgaatccct tctgtcgaaa aagtcatggt cctacattgc cgaggccccg     300

aatgctgaga acggcatttg ctacccggga cacttcagcg attacgaaga actgcgggaa     360

cagctgtcga gcgtgtcaag tttcgagagg ttcgagatct tcccgaagga aagctcatgg     420

ccgaagcaca gcatcggcgc caccgcctcg tgttcgaaac agggaaagtc ctcgttctac     480

actaacctcc tgtggctgac cgagaagaat gggtcctatc ctaacctgac catgtcgtac     540

atgaacgaca aggaacggga agtgctcgtg ttgtgggggg tgcaccatcc atcaaatatc     600

gaggaccaga gggccatcta ccgcaaagaa actgcctacg tgtccgtgat gtctagccac     660

tacaacaagc gcttcacgcc tgaaatcgcc aaaagaccca aggtccgcaa ccaagagggc     720

agaatcaatt actactggac cctcctggag ccccgggaca ccataatctt tgaagcaaat     780

ggaaacctca ttgcgccttg gtacgccttc gccctgaacc ggggtttcga gtccggaatt     840

atcgtcagca acgcctccat ggacgagtgt aacgcgaagt gccagacccc gcgcggcgct     900

atcaacaaca gcctgccttt ccaaaacgtg cacccaatca ccatcggcga atgccccaaa     960

tacgtgaagt ccactaagct gaagatggca acaggactgc ggaacatccc gtcaattcag    1020

tcccgagggt tgttcggggc aatcgccggg ttcattgagg gcggatggac cggaatgatt    1080

gacggttggt acggatacca ccaccagaac gaacagggtt ccggttatgc ggcggatcag    1140

aagtccaccc agaacgccat caacggcatc accaacaagg tcaactccgt catcgacaag    1200

atgaacaccc agttcactgc cgtgggaaag gagttcaacc ggctggagaa gcggatggaa    1260

aatctgaaca agaaagtcga cgacggcttc ctggacattt ggacctacaa cgccgaactg    1320

ctggtgctgc tggaaaacga aagaactctc gactttcatg attcgaacgt gaagtcactg    1380

tacgaaggag tgaaggccca gctcaagaac aacgccaagg aagtcggaaa cggttgcttc    1440

gagttttacc ataagtgcga caacgagtgc atggatagcg tgaagaacgg aacctacgac    1500

tacccccggt attccgaaga gtcgaagctc aacagagaac agatcgatgg cgtggagctg    1560

aagtccatgg gagtgtacca gatcctggcg atctactcca ctgcggctag ctccctggtg    1620

ctgttggtca ccttgggggc tatctctttc tggatgtgct ccaacggttc cctgcaatgc    1680

cgcatttgca tttga                                                     1695


<210> 3
<211> 564
<212> PRT
<213> Influenza A virus


<220> 
<223> >ALX30160.1 hemagglutinin [Influenza A virus
      (A/swine/Italy/3033-1/2015(H1N2))]

<400> 3
Met Lys Ala Lys Leu Leu Ile Leu Leu Cys Ala Leu Ser Thr Thr Asp 
1               5                   10                  15      
Ala Asp Thr Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 
            20                  25                  30          
Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
        35                  40                  45              
Leu Leu Glu Asp Ser His Asn Gly Lys Leu Cys Lys Leu Lys Gly Val 
    50                  55                  60                  
Ala Pro Leu Gln Leu Gly Lys Cys Ser Ile Ala Gly Trp Ile Leu Gly 
65                  70                  75                  80  
Asn Pro Glu Cys Glu Ser Leu Leu Ser Lys Lys Ser Trp Ser Tyr Ile 
                85                  90                  95      
Ala Glu Ala Pro Asn Ala Glu Asn Gly Ile Cys Tyr Pro Gly His Phe 
            100                 105                 110         
Ser Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe 
        115                 120                 125             
Glu Arg Phe Glu Ile Phe Pro Lys Glu Ser Ser Trp Pro Lys His Ser 
    130                 135                 140                 
Ile Gly Ala Thr Ala Ser Cys Ser Lys Gln Gly Lys Ser Ser Phe Tyr 
145                 150                 155                 160 
Thr Asn Leu Leu Trp Leu Thr Glu Lys Asn Gly Ser Tyr Pro Asn Leu 
                165                 170                 175     
Thr Met Ser Tyr Met Asn Asp Lys Glu Arg Glu Val Leu Val Leu Trp 
            180                 185                 190         
Gly Val His His Pro Ser Asn Ile Glu Asp Gln Arg Ala Ile Tyr Arg 
        195                 200                 205             
Lys Glu Thr Ala Tyr Val Ser Val Met Ser Ser His Tyr Asn Lys Arg 
    210                 215                 220                 
Phe Thr Pro Glu Ile Ala Lys Arg Pro Lys Val Arg Asn Gln Glu Gly 
225                 230                 235                 240 
Arg Ile Asn Tyr Tyr Trp Thr Leu Leu Glu Pro Arg Asp Thr Ile Ile 
                245                 250                 255     
Phe Glu Ala Asn Gly Asn Leu Ile Ala Pro Trp Tyr Ala Phe Ala Leu 
            260                 265                 270         
Asn Arg Gly Phe Glu Ser Gly Ile Ile Val Ser Asn Ala Ser Met Asp 
        275                 280                 285             
Glu Cys Asn Ala Lys Cys Gln Thr Pro Arg Gly Ala Ile Asn Asn Ser 
    290                 295                 300                 
Leu Pro Phe Gln Asn Val His Pro Ile Thr Ile Gly Glu Cys Pro Lys 
305                 310                 315                 320 
Tyr Val Lys Ser Thr Lys Leu Lys Met Ala Thr Gly Leu Arg Asn Ile 
                325                 330                 335     
Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile 
            340                 345                 350         
Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His 
        355                 360                 365             
Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Gln Lys Ser Thr Gln 
    370                 375                 380                 
Asn Ala Ile Asn Gly Ile Thr Asn Lys Val Asn Ser Val Ile Asp Lys 
385                 390                 395                 400 
Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn Arg Leu Glu 
                405                 410                 415     
Lys Arg Met Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe Leu Asp 
            420                 425                 430         
Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Glu Arg 
        435                 440                 445             
Thr Leu Asp Phe His Asp Ser Asn Val Lys Ser Leu Tyr Glu Gly Val 
    450                 455                 460                 
Lys Ala Gln Leu Lys Asn Asn Ala Lys Glu Val Gly Asn Gly Cys Phe 
465                 470                 475                 480 
Glu Phe Tyr His Lys Cys Asp Asn Glu Cys Met Asp Ser Val Lys Asn 
                485                 490                 495     
Gly Thr Tyr Asp Tyr Pro Arg Tyr Ser Glu Glu Ser Lys Leu Asn Arg 
            500                 505                 510         
Glu Gln Ile Asp Gly Val Glu Leu Lys Ser Met Gly Val Tyr Gln Ile 
        515                 520                 525             
Leu Ala Ile Tyr Ser Thr Ala Ala Ser Ser Leu Val Leu Leu Val Thr 
    530                 535                 540                 
Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys 
545                 550                 555                 560 
Arg Ile Cys Ile 
                

<210> 4
<211> 1701
<212> DNA
<213> Influenza A virus


<220> 
<223> >KR700530.1 Influenza A virus (A/swine/Italy/28762-3/2013(H1N1))
      segment 4 hemagglutinin (HA) gene, complete cds

<400> 4
atgaaagcaa aattatttgt attattctgt gcatttactg cactgaaagc tgacaccatt      60

tgtgtaggct atcatgctaa caattccaca gacactgtcg acacgatact ggaaaagaat     120

gttactgtta cccactcagt taatttacta gaaaacagcc acaatggaaa actctgcagc     180

ctgaatggaa aggctccgtt gcaactggag aactgcaacg tagcaggatg gatccttggt     240

aatccagaat gtgactttct gctcacagcg aattcatggt cctacataat agagacttca     300

aattcaaaaa atggaacatg ctatcctgga gaattcactg attatgagga actaagggag     360

caactgagta cagtttcttc atttgaaaga tttgaaattt tcccaaaagc aacctcatgg     420

ccaaatcatg agacaaccaa aggtaccaca gttgcatgct cccactctgg agccaacagt     480

ttttatcgga acttgctatg gatagtaaag aaaggaaact cctatcccaa gctcagcaag     540

tcatacacaa acaacaaagg gaaagaagtg cttgtaatct ggggagtgca tcacccccct     600

acagacagtg accaacaaac cctctatcag aataatcaca catatgtttc agttggatca     660

tcaaaatact accaaaggtt cacaccagaa atagtagcca gacctaaagt cagagagcaa     720

gcgggcagaa tgaattatta ttggatacta ttagatcaag gagacaccat aacatttgaa     780

gccacgggga atttaatagc accatggcat gcatttgcat tgaataaggg ctctagctct     840

ggaattatga tgtcagatgc tcatgttcaa aattgcacta caaagtgcca aactcctcat     900

ggggcattga aaagcaatct tccttttcaa aatgtacatc ccatcactat tggagagtgc     960

cccaaatatg ttaaaagcac ccaactaaga atggcaacag gattaaggaa cataccatct    1020

atccaatcca gaggactttt tggggcaatt gccggattta ttgaaggagg atggacagga    1080

atgatagatg gatggtatgg atatcaccat caaaatgagc aaggatctgg ttatgcagca    1140

gataagaaaa gcacacaaat tgcaattgat gagatcagca acaaagtaaa ctcagtaatt    1200

gagaaaatga acattcaatt cacttcagtg ggcaaggagt tcaataattt agagaaaagg    1260

attgagaatt tgaataaaaa ggtcgatgat ggatttttgg atgtgtggac atataacgct    1320

gagttactca ttttgctcga gaacgaaaga accctagatt tccatgactt taacgtaaga    1380

aatttgtatg aaaaggtcaa atcacaactg agaaacaatg ccaaggaaat cggtaatggt    1440

tgttttgagt tctatcacag atgtgacaat gaatgcatgg aaagtgtaaa gaatggcaca    1500

tataattatc ccagatattc agaagaatcc aaattaaata gagagaaaat agacggtgtg    1560

aaactagaat cattaggagt acatcagatt ttggcaatct actccacagt cgccagttcc    1620

ctggtattgc tagtctccct gggggcaatc agtttctgga tgtgctctaa tggatcattg    1680

caatgcagaa tatgcatttg a                                              1701


<210> 5
<211> 1701
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUH1-3 Swine optimized sequence

<400> 5
atgaaagcca agctgtttgt ccttttctgc gccttcaccg cccttaaagc cgacaccatc      60

tgtgtgggct accatgctaa caactccacc gatacggtcg acactattct ggagaagaac     120

gtgaccgtga cccactccgt gaatctgctg gagaatagcc acaacggaaa gctgtgcagc     180

ctgaacggaa aggccccgct gcaactggag aattgtaacg tggccggatg gatcctgggc     240

aaccctgaat gcgacttcct gctgaccgcc aattcctggt cctacatcat cgagacttcc     300

aactccaaga acggaacgtg ctaccccggg gagttcactg attacgaaga actgcgcgaa     360

cagctgtcaa ctgtgtcctc gttcgagcgg ttcgaaatct tcccgaaagc cacctcgtgg     420

cccaatcatg aaaccaccaa gggaaccact gtggcctgct cccactctgg ggcgaactca     480

ttctatcgca acttgctgtg gatcgtcaag aagggaaaca gttaccctaa gctgtccaag     540

tcctacacta acaacaaggg aaaggaagtc ctcgtgatct ggggcgtgca tcacccaccc     600

accgactccg atcagcaaac actgtaccag aacaaccaca cctacgtgtc cgtgggatca     660

tccaagtact accagagatt caccccggaa atcgtcgcgc ggcccaaggt ccgcgaacaa     720

gccggccgga tgaactacta ctggatcctg ctggaccagg gcgacactat cactttcgag     780

gcgaccggga acctcatcgc cccttggcac gcattcgcac tgaacaaggg atcgagctcc     840

gggattatga tgagcgatgc tcatgtgcaa aactgtacta ccaagtgcca gacccctcac     900

ggtgccctga agtccaattt gcccttccaa aatgtgcacc ccattaccat tggcgaatgc     960

ccgaaatacg tgaagtcgac ccaactccgg atggccaccg gccttcggaa catcccatcg    1020

atacagtcga ggggtctttt cggagcgatt gcgggattca tcgagggcgg ctggaccgga    1080

atgattgacg gttggtacgg ataccaccac cagaacgaac agggctccgg atacgccgcc    1140

gacaaaaagt cgactcagat cgcaattgac gagattagca acaaggtcaa ctccgtgatc    1200

gagaagatga atatccagtt tactagcgtg ggaaaggagt tcaacaacct ggagaagagg    1260

atcgagaacc tgaacaagaa agtggacgat ggattcctcg acgtgtggac atacaacgcc    1320

gagctcctca ttttgctgga gaacgaacgg accctcgact tccatgactt caacgtgcgc    1380

aacctgtacg aaaaggtcaa gagccagctg agaaacaacg ccaaggaaat tggcaacggt    1440

tgctttgagt tttaccaccg ctgcgacaac gaatgcatgg agagcgtgaa gaacgggacc    1500

tacaactatc cgagatactc agaggaatca aagctgaacc gcgaaaagat cgatggcgtg    1560

aagctcgaat cactcggcgt gcaccagatc ctggccatct attccactgt ggctagctcc    1620

ctcgtgctgc tggtgtccct gggtgctatt tccttctgga tgtgcagcaa cgggtcgctc    1680

cagtgccgga tctgtatctg a                                              1701


<210> 6
<211> 566
<212> PRT
<213> Influenza A virus


<220> 
<223> >AKJ81667.1 hemagglutinin [Influenza A virus
      (A/swine/Italy/28762-3/2013(H1N1))]

<400> 6
Met Lys Ala Lys Leu Phe Val Leu Phe Cys Ala Phe Thr Ala Leu Lys 
1               5                   10                  15      
Ala Asp Thr Ile Cys Val Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 
            20                  25                  30          
Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
        35                  40                  45              
Leu Leu Glu Asn Ser His Asn Gly Lys Leu Cys Ser Leu Asn Gly Lys 
    50                  55                  60                  
Ala Pro Leu Gln Leu Glu Asn Cys Asn Val Ala Gly Trp Ile Leu Gly 
65                  70                  75                  80  
Asn Pro Glu Cys Asp Phe Leu Leu Thr Ala Asn Ser Trp Ser Tyr Ile 
                85                  90                  95      
Ile Glu Thr Ser Asn Ser Lys Asn Gly Thr Cys Tyr Pro Gly Glu Phe 
            100                 105                 110         
Thr Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Thr Val Ser Ser Phe 
        115                 120                 125             
Glu Arg Phe Glu Ile Phe Pro Lys Ala Thr Ser Trp Pro Asn His Glu 
    130                 135                 140                 
Thr Thr Lys Gly Thr Thr Val Ala Cys Ser His Ser Gly Ala Asn Ser 
145                 150                 155                 160 
Phe Tyr Arg Asn Leu Leu Trp Ile Val Lys Lys Gly Asn Ser Tyr Pro 
                165                 170                 175     
Lys Leu Ser Lys Ser Tyr Thr Asn Asn Lys Gly Lys Glu Val Leu Val 
            180                 185                 190         
Ile Trp Gly Val His His Pro Pro Thr Asp Ser Asp Gln Gln Thr Leu 
        195                 200                 205             
Tyr Gln Asn Asn His Thr Tyr Val Ser Val Gly Ser Ser Lys Tyr Tyr 
    210                 215                 220                 
Gln Arg Phe Thr Pro Glu Ile Val Ala Arg Pro Lys Val Arg Glu Gln 
225                 230                 235                 240 
Ala Gly Arg Met Asn Tyr Tyr Trp Ile Leu Leu Asp Gln Gly Asp Thr 
                245                 250                 255     
Ile Thr Phe Glu Ala Thr Gly Asn Leu Ile Ala Pro Trp His Ala Phe 
            260                 265                 270         
Ala Leu Asn Lys Gly Ser Ser Ser Gly Ile Met Met Ser Asp Ala His 
        275                 280                 285             
Val Gln Asn Cys Thr Thr Lys Cys Gln Thr Pro His Gly Ala Leu Lys 
    290                 295                 300                 
Ser Asn Leu Pro Phe Gln Asn Val His Pro Ile Thr Ile Gly Glu Cys 
305                 310                 315                 320 
Pro Lys Tyr Val Lys Ser Thr Gln Leu Arg Met Ala Thr Gly Leu Arg 
                325                 330                 335     
Asn Ile Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly 
            340                 345                 350         
Phe Ile Glu Gly Gly Trp Thr Gly Met Ile Asp Gly Trp Tyr Gly Tyr 
        355                 360                 365             
His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Lys Ser 
    370                 375                 380                 
Thr Gln Ile Ala Ile Asp Glu Ile Ser Asn Lys Val Asn Ser Val Ile 
385                 390                 395                 400 
Glu Lys Met Asn Ile Gln Phe Thr Ser Val Gly Lys Glu Phe Asn Asn 
                405                 410                 415     
Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe 
            420                 425                 430         
Leu Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Ile Leu Leu Glu Asn 
        435                 440                 445             
Glu Arg Thr Leu Asp Phe His Asp Phe Asn Val Arg Asn Leu Tyr Glu 
    450                 455                 460                 
Lys Val Lys Ser Gln Leu Arg Asn Asn Ala Lys Glu Ile Gly Asn Gly 
465                 470                 475                 480 
Cys Phe Glu Phe Tyr His Arg Cys Asp Asn Glu Cys Met Glu Ser Val 
                485                 490                 495     
Lys Asn Gly Thr Tyr Asn Tyr Pro Arg Tyr Ser Glu Glu Ser Lys Leu 
            500                 505                 510         
Asn Arg Glu Lys Ile Asp Gly Val Lys Leu Glu Ser Leu Gly Val His 
        515                 520                 525             
Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu 
    530                 535                 540                 
Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu 
545                 550                 555                 560 
Gln Cys Arg Ile Cys Ile 
                565     

<210> 7
<211> 1701
<212> DNA
<213> Influenza A virus


<220> 
<223> >KU323308.1 Influenza A virus (A/swine/Italy/240849/2015(H3N2))
      segment 4 hemagglutinin (HA) gene, complete cds

<400> 7
atgaagactg tcattgcttt aagctacgtc ttttgcctgg tttttggcca agattttcca      60

ggaaagggca acaacacagc aacactgtgc ctagggcatc atgcagtgcc aaatgggaca     120

ttagtgaaaa caatcacaga tgatcaaatc gaagtaacca atgccacaga actggtccaa     180

aatttctcaa tgggtaaaat atgcaagaat cctcatcgaa ttcttgatgg ggcaaattgt     240

acattggtag actctctatt gggggaccct cactgtgatg gttttcaaaa tgagaaatgg     300

gacctcttca ttgaacgcag cagggctttc agcaactgct acccttatga tgtgccggag     360

tatacctctc ttaggtcact aattgcctca tcaggcacct tggaatttac caatgaaaat     420

ttcaattgga ctggagttac ccaaaatggg ggaagcagtg cttgcaagag ggggcccaac     480

aacagctttt tcagtagatt gaactggttg tacaaatcag gaaacacata tccgatgcta     540

aatgttacta tgccaaacag tgatgatttc gacaaattgt acatttgggg agttcatcat     600

ccgagcaccg atagagaaca gaccaacctg tacattcaag catcagggaa aataatagta     660

tccacaaaga gaagtcaaca aaccataatc ccgaatatcg ggtctagacc ctgggtaagg     720

ggtttatcta gtaggataag catctactgg acaatagtta aaccaggaga catcctgata     780

atcaacagca atggaaacct aattgctcct cggggttact tcaagataca gactgggaaa     840

agttcagtaa tgaagtcaga tgcacctatt ggcacctgca attcagaatg cattactcca     900

aacggaagca tacccaatga taaacccttt caaaacgtaa acaggatcac atatggggca     960

tgccctcatt acatcaagca aaacactctg aagctagcaa caggaatgcg gaatatacca    1020

gaaagacaaa ccagaggtat attcggtgca atagcagggt tcatagagaa tggttgggaa    1080

ggaatggtga acggttggta cggtttcaga caccaaaatt ctgagggcat aggacaggca    1140

gcagatctta aaagtaccca agcagctatc aaccaaatca atggaaaact gaatagagta    1200

atcgagaaaa caaacgagaa attccatcaa atcgaaaagg agttctcaga agtagaagga    1260

aggatccaag accttgaaag atatgttgaa gatactaaga tagatctttg gtcttacaac    1320

gcggaacttc ttgtcgcact agagaatcaa cacacaattg acctgactga ctcagaaatg    1380

aacaaactct ttgaaaaaac aaggaaacaa ctaagggaaa atgctgagga catgggaaat    1440

ggttgcttca aaatatacca caaatgtgac aactcttgca tggagtcaat cagaaatggg    1500

acttacgacc ataatgaata cagagacgaa gcagtaaaca atcgattcca aatcaaaagt    1560

gttgagctga agtcaggata caaagactgg atcctgtgga tttcctttgc catatcatgc    1620

tttttgcttt gcgctatttg gatgggattc gtaatatggg cctgccagaa aggcaacatt    1680

agatgcaata tttgcatctg a                                              1701


<210> 8
<211> 1701
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUH3-4_Swine optimized sequence

<400> 8
atgaaaaccg tcattgcgtt gagctatgtg ttttgtctcg tgtttggaca agacttcccg      60

ggaaagggca acaacaccgc caccctgtgc ctggggcacc atgccgtgcc caacggaact     120

ctcgtcaaga ccatcaccga cgaccagatc gaggtcacca atgcgaccga gttggtgcag     180

aatttctcca tgggaaagat ctgcaagaac ccgcaccgga ttttggatgg agccaactgc     240

acactggtgg actccctgct gggtgatcct cattgcgacg gattccaaaa cgagaagtgg     300

gatctgttca tcgagagatc ccgggccttc agcaactgct acccgtacga cgtgccagag     360

tacactagct tgcggtccct gatcgcctcc agcggtaccc tggagttcac caacgagaac     420

ttcaactgga ccggcgtgac gcagaacggg ggttcctcag cgtgcaagcg gggaccgaac     480

aactcgttct tctcccggct taactggctg tacaagagcg gcaacacata ccccatgctg     540

aacgtgacca tgcccaactc tgacgacttt gataagctgt acatctgggg agtgcaccac     600

ccatcgactg accgcgaaca gaccaacctg tacatccaag cctccggcaa aatcattgtg     660

tccaccaaga gatcgcagca gaccatcatt ccgaacattg gcagtcggcc atgggtcaga     720

ggactgtcgt ccagaatcag catctactgg accattgtga agcccggcga catcctcatc     780

ataaactcga acggcaacct gatcgcccca cggggatatt tcaagatcca gaccggaaag     840

agctccgtga tgaagtccga tgccccgatt gggacttgca actccgagtg catcaccccc     900

aacggctcca tccccaacga caaacccttc caaaacgtga accgcatcac ttacggagcc     960

tgcccgcatt acatcaagca gaacacgctc aagctcgcca ctgggatgcg caacattcct    1020

gagcgccaaa cccggggaat cttcggagcc attgcgggct tcatcgaaaa cggctgggaa    1080

gggatggtca acggttggta cggttttaga caccaaaatt ccgaaggaat cggacaggct    1140

gccgatctca aatcaaccca ggccgccatc aaccagatca acgggaagct gaacagagtg    1200

atcgaaaaga ccaacgaaaa gttccatcag attgagaagg agttttccga agtcgaggga    1260

cggattcaag acctggagcg ctacgtggag gacaccaaga ttgatctgtg gagctacaac    1320

gcagagctcc tggtcgcgct ggaaaatcag cacaccattg acttgaccga ctcggagatg    1380

aacaagctgt ttgaaaagac caggaagcag ctgcgggaaa acgccgaaga tatggggaac    1440

ggatgcttca aaatctacca taagtgcgac aatagctgca tggaaagcat taggaacggg    1500

acttacgacc ataacgagta cagagatgaa gcagtgaaca acagattcca gatcaagtcc    1560

gtggaactca agagcggtta caaggactgg atcctctgga tttcgttcgc tatttcgtgc    1620

ttcctgctgt gcgccatctg gatgggattc gtcatctggg cctgccagaa gggcaacatc    1680

cggtgcaaca tttgtatttg a                                              1701


<210> 9
<211> 566
<212> PRT
<213> Influenza A virus


<220> 
<223> >ALX30415.1 hemagglutinin [Influenza A virus
      (A/swine/Italy/240849/2015(H3N2))] EUH3-4

<400> 9
Met Lys Thr Val Ile Ala Leu Ser Tyr Val Phe Cys Leu Val Phe Gly 
1               5                   10                  15      
Gln Asp Phe Pro Gly Lys Gly Asn Asn Thr Ala Thr Leu Cys Leu Gly 
            20                  25                  30          
His His Ala Val Pro Asn Gly Thr Leu Val Lys Thr Ile Thr Asp Asp 
        35                  40                  45              
Gln Ile Glu Val Thr Asn Ala Thr Glu Leu Val Gln Asn Phe Ser Met 
    50                  55                  60                  
Gly Lys Ile Cys Lys Asn Pro His Arg Ile Leu Asp Gly Ala Asn Cys 
65                  70                  75                  80  
Thr Leu Val Asp Ser Leu Leu Gly Asp Pro His Cys Asp Gly Phe Gln 
                85                  90                  95      
Asn Glu Lys Trp Asp Leu Phe Ile Glu Arg Ser Arg Ala Phe Ser Asn 
            100                 105                 110         
Cys Tyr Pro Tyr Asp Val Pro Glu Tyr Thr Ser Leu Arg Ser Leu Ile 
        115                 120                 125             
Ala Ser Ser Gly Thr Leu Glu Phe Thr Asn Glu Asn Phe Asn Trp Thr 
    130                 135                 140                 
Gly Val Thr Gln Asn Gly Gly Ser Ser Ala Cys Lys Arg Gly Pro Asn 
145                 150                 155                 160 
Asn Ser Phe Phe Ser Arg Leu Asn Trp Leu Tyr Lys Ser Gly Asn Thr 
                165                 170                 175     
Tyr Pro Met Leu Asn Val Thr Met Pro Asn Ser Asp Asp Phe Asp Lys 
            180                 185                 190         
Leu Tyr Ile Trp Gly Val His His Pro Ser Thr Asp Arg Glu Gln Thr 
        195                 200                 205             
Asn Leu Tyr Ile Gln Ala Ser Gly Lys Ile Ile Val Ser Thr Lys Arg 
    210                 215                 220                 
Ser Gln Gln Thr Ile Ile Pro Asn Ile Gly Ser Arg Pro Trp Val Arg 
225                 230                 235                 240 
Gly Leu Ser Ser Arg Ile Ser Ile Tyr Trp Thr Ile Val Lys Pro Gly 
                245                 250                 255     
Asp Ile Leu Ile Ile Asn Ser Asn Gly Asn Leu Ile Ala Pro Arg Gly 
            260                 265                 270         
Tyr Phe Lys Ile Gln Thr Gly Lys Ser Ser Val Met Lys Ser Asp Ala 
        275                 280                 285             
Pro Ile Gly Thr Cys Asn Ser Glu Cys Ile Thr Pro Asn Gly Ser Ile 
    290                 295                 300                 
Pro Asn Asp Lys Pro Phe Gln Asn Val Asn Arg Ile Thr Tyr Gly Ala 
305                 310                 315                 320 
Cys Pro His Tyr Ile Lys Gln Asn Thr Leu Lys Leu Ala Thr Gly Met 
                325                 330                 335     
Arg Asn Ile Pro Glu Arg Gln Thr Arg Gly Ile Phe Gly Ala Ile Ala 
            340                 345                 350         
Gly Phe Ile Glu Asn Gly Trp Glu Gly Met Val Asn Gly Trp Tyr Gly 
        355                 360                 365             
Phe Arg His Gln Asn Ser Glu Gly Ile Gly Gln Ala Ala Asp Leu Lys 
    370                 375                 380                 
Ser Thr Gln Ala Ala Ile Asn Gln Ile Asn Gly Lys Leu Asn Arg Val 
385                 390                 395                 400 
Ile Glu Lys Thr Asn Glu Lys Phe His Gln Ile Glu Lys Glu Phe Ser 
                405                 410                 415     
Glu Val Glu Gly Arg Ile Gln Asp Leu Glu Arg Tyr Val Glu Asp Thr 
            420                 425                 430         
Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Leu Glu 
        435                 440                 445             
Asn Gln His Thr Ile Asp Leu Thr Asp Ser Glu Met Asn Lys Leu Phe 
    450                 455                 460                 
Glu Lys Thr Arg Lys Gln Leu Arg Glu Asn Ala Glu Asp Met Gly Asn 
465                 470                 475                 480 
Gly Cys Phe Lys Ile Tyr His Lys Cys Asp Asn Ser Cys Met Glu Ser 
                485                 490                 495     
Ile Arg Asn Gly Thr Tyr Asp His Asn Glu Tyr Arg Asp Glu Ala Val 
            500                 505                 510         
Asn Asn Arg Phe Gln Ile Lys Ser Val Glu Leu Lys Ser Gly Tyr Lys 
        515                 520                 525             
Asp Trp Ile Leu Trp Ile Ser Phe Ala Ile Ser Cys Phe Leu Leu Cys 
    530                 535                 540                 
Ala Ile Trp Met Gly Phe Val Ile Trp Ala Cys Gln Lys Gly Asn Ile 
545                 550                 555                 560 
Arg Cys Asn Ile Cys Ile 
                565     

<210> 10
<211> 1701
<212> DNA
<213> Influenza A virus


<220> 
<223> >CY116127.1 Influenza A virus (A/swine/England/373/2010(H1N1))
      hemagglutinin (HA) gene, complete cds

<400> 10
atgaaggcaa tactagtagt tctgctacat acatttgcaa ccgcaaatgc agacacatta      60

tgtataggtt atcatgcgaa caattcaaca gacactgtag acacagtact agaaaagaat     120

gtaacagtaa cacactctgt taaccttcta gaagacaagc ataacgggaa actatgcaaa     180

ctaagagggg tagccccatt gcatttgggt aaatgtaaca ttgctggctg gatcctggga     240

aatccagagt gtgaatcact ctccacagca agctcatggt cctacattgt ggaaacatct     300

agttcagaca atggaacgtg ttacccagga gatttcatcg attatgagga gctaagagag     360

caattgagct cagtgtcatc atttgaaagg tttgagatat tccccaagac aagttcatgg     420

cccaatcatg actcgaacaa aggtgtaacg gcagcatgtc ctcatgctgg agcaaaaagc     480

ttctacaaaa atttaatatg gctagttaaa aaaggaaatt catacccaaa gctcagcaaa     540

tcctacatta atgataaagg gaaagaagtc ctcgtgctat ggggcattca ccatccatct     600

actagtgctg accaacaaag tctctatcag aatgcagatg catatgtttt tgtggggaca     660

tcaagataca gcaagaagtt caagccggaa atagcaataa gacccaaagt gagggatcaa     720

gaagggagaa tgaactatta ctggacacta gtagagccgg gagacaaaat aacattcgaa     780

gcaactggaa atctagtggt accgagatat gcattcgcaa tggaaagaaa tgctggatct     840

ggtattatca tttcagatac accagtccac gattgcaata caacttgtca gacacccaag     900

ggtgctataa acaccagcct cccatttcag aatatacatc cgatcacaat tggaaaatgt     960

ccaaaatatg taaaaagcac aaaattgaga ctggccacag gattgaggaa cgtcccgtct    1020

attcaatcta gaggcctatt tggggccatt gccggtttca ttgaaggggg gtggacaggg    1080

atggtagatg gatggtacgg ttatcaccat caaaatgagc aggggtcagg atatgcagcc    1140

gacctgaaga gcacacagca tgccattgac gagattacta acaaagtaaa ttctgttatt    1200

gaaaagatga atacacagtt cacagcagta ggtaaagagt tcaaccacct ggaaaaaaga    1260

atagagaatt taaataaaaa agttgatgat ggtttcctgg acatttggac ttacaatgcc    1320

gaactgttgg ttctattgga aaatgaaaga actttggact accacgattc aaatgtgaag    1380

aacttatatg aaaaggtaag aagccagtta aaaaacaatg ccaaggaaat tggaaacggc    1440

tgctttgaat tttaccacaa atgcgataac acgtgcatgg aaagtgtcaa aaatgggact    1500

tatgactacc caaaatactc agaggaagca aaattaaaca gagaagaaat agatggggta    1560

aagctagaat caacaaggat ttaccagatt ttggcgatct attcaactgt cgccagttca    1620

ttggtactgg tagtctccct gggggcaatc agtttctgga tgtgctctaa tgggtctcta    1680

cagtgtagaa tatgtattta a                                              1701


<210> 11
<211> 1701
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUH1-11 Swine optimized sequence

<400> 11
atgaaggcta ttctggtggt cctgctccac acttttgcca ccgcgaacgc agataccctc      60

tgcatcggat accacgccaa caattccacc gatactgtgg ataccgtgct ggaaaagaac     120

gtgaccgtga ctcactcagt gaacctcctg gaagataagc acaacggaaa gctttgcaag     180

cttcggggag tggctccgtt gcatctggga aagtgcaaca tcgccggatg gatcctgggc     240

aaccccgagt gcgagagcct gtccaccgcc tcctcctggt cttacattgt ggaaacctcc     300

tcgtccgata acggcacatg ctatccgggg gacttcattg actacgaaga actgagggag     360

caactcagct ccgtgagctc ctttgagcgc ttcgaaatct tccctaagac ctcatcctgg     420

cccaaccacg actccaacaa gggagtgact gcagcgtgtc cgcacgctgg agccaagtca     480

ttctacaaga acctgatctg gctggtcaaa aagggaaact cgtaccccaa gctgtctaag     540

tcctacatca atgacaaggg gaaggaagtg ctggtgctgt ggggaatcca ccacccttcc     600

acctccgccg accagcagtc gctgtaccag aacgctgatg cgtacgtgtt cgtcggcact     660

agccgctact ccaagaagtt caagccagaa attgccatcc ggcccaaagt ccgcgatcag     720

gaggggcgga tgaactacta ctggactctc gtggaacccg gggataagat cacgttcgaa     780

gctaccggca acctcgtggt gcctcgctac gccttcgcaa tggagaggaa tgctggaagc     840

ggtatcatca tttcggacac tcctgtgcat gactgcaata cgacttgtca gactccgaag     900

ggagcgatca acacttccct cccgttccaa aacatccacc ccattacaat tggcaaatgt     960

cctaaatacg tgaagtcgac caagctccgg cttgcaactg ggctgcgcaa tgtgcctagc    1020

attcagtcaa ggggcctatt tggggccatc gccggtttca tcgagggagg atggaccgga    1080

atggtggacg gttggtatgg ctaccaccac caaaacgaac agggctccgg ttacgccgcc    1140

gacctgaagt ccacccagca cgccattgac gaaatcacca acaaggtcaa ctccgtcatc    1200

gagaagatga acacccagtt cactgccgtg ggaaaggagt tcaaccatct ggagaagaga    1260

attgagaacc tcaataagaa agtggacgac ggcttcctgg acatctggac ctacaacgcc    1320

gagctgctgg tcctgctgga aaacgagagg accctggact accacgactc gaacgtgaag    1380

aacctgtacg agaaggtccg atcacaactg aagaacaacg ccaaggaaat cggaaatggg    1440

tgcttcgagt tctaccacaa atgcgacaat acttgcatgg aatccgtgaa gaatggcacc    1500

tacgattacc ctaagtattc cgaggaagcg aagctgaacc gggaagagat tgacggcgtg    1560

aagctcgaat caacccggat ctaccagatc cttgcgatct actccaccgt ggccagctca    1620

ctcgtgctcg tggtgtccct gggtgccatc tccttctgga tgtgtagcaa cggcagcctc    1680

cagtgccgca tctgtatctg a                                              1701


<210> 12
<211> 566
<212> PRT
<213> Influenza A virus


<220> 
<223> >AFR76205.1 hemagglutinin [Influenza A virus
      (A/swine/England/373/2010(H1N1) EUH1-11

<400> 12
Met Lys Ala Ile Leu Val Val Leu Leu His Thr Phe Ala Thr Ala Asn 
1               5                   10                  15      
Ala Asp Thr Leu Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Asp Thr 
            20                  25                  30          
Val Asp Thr Val Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
        35                  40                  45              
Leu Leu Glu Asp Lys His Asn Gly Lys Leu Cys Lys Leu Arg Gly Val 
    50                  55                  60                  
Ala Pro Leu His Leu Gly Lys Cys Asn Ile Ala Gly Trp Ile Leu Gly 
65                  70                  75                  80  
Asn Pro Glu Cys Glu Ser Leu Ser Thr Ala Ser Ser Trp Ser Tyr Ile 
                85                  90                  95      
Val Glu Thr Ser Ser Ser Asp Asn Gly Thr Cys Tyr Pro Gly Asp Phe 
            100                 105                 110         
Ile Asp Tyr Glu Glu Leu Arg Glu Gln Leu Ser Ser Val Ser Ser Phe 
        115                 120                 125             
Glu Arg Phe Glu Ile Phe Pro Lys Thr Ser Ser Trp Pro Asn His Asp 
    130                 135                 140                 
Ser Asn Lys Gly Val Thr Ala Ala Cys Pro His Ala Gly Ala Lys Ser 
145                 150                 155                 160 
Phe Tyr Lys Asn Leu Ile Trp Leu Val Lys Lys Gly Asn Ser Tyr Pro 
                165                 170                 175     
Lys Leu Ser Lys Ser Tyr Ile Asn Asp Lys Gly Lys Glu Val Leu Val 
            180                 185                 190         
Leu Trp Gly Ile His His Pro Ser Thr Ser Ala Asp Gln Gln Ser Leu 
        195                 200                 205             
Tyr Gln Asn Ala Asp Ala Tyr Val Phe Val Gly Thr Ser Arg Tyr Ser 
    210                 215                 220                 
Lys Lys Phe Lys Pro Glu Ile Ala Ile Arg Pro Lys Val Arg Asp Gln 
225                 230                 235                 240 
Glu Gly Arg Met Asn Tyr Tyr Trp Thr Leu Val Glu Pro Gly Asp Lys 
                245                 250                 255     
Ile Thr Phe Glu Ala Thr Gly Asn Leu Val Val Pro Arg Tyr Ala Phe 
            260                 265                 270         
Ala Met Glu Arg Asn Ala Gly Ser Gly Ile Ile Ile Ser Asp Thr Pro 
        275                 280                 285             
Val His Asp Cys Asn Thr Thr Cys Gln Thr Pro Lys Gly Ala Ile Asn 
    290                 295                 300                 
Thr Ser Leu Pro Phe Gln Asn Ile His Pro Ile Thr Ile Gly Lys Cys 
305                 310                 315                 320 
Pro Lys Tyr Val Lys Ser Thr Lys Leu Arg Leu Ala Thr Gly Leu Arg 
                325                 330                 335     
Asn Val Pro Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly 
            340                 345                 350         
Phe Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr 
        355                 360                 365             
His His Gln Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Leu Lys Ser 
    370                 375                 380                 
Thr Gln His Ala Ile Asp Glu Ile Thr Asn Lys Val Asn Ser Val Ile 
385                 390                 395                 400 
Glu Lys Met Asn Thr Gln Phe Thr Ala Val Gly Lys Glu Phe Asn His 
                405                 410                 415     
Leu Glu Lys Arg Ile Glu Asn Leu Asn Lys Lys Val Asp Asp Gly Phe 
            420                 425                 430         
Leu Asp Ile Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn 
        435                 440                 445             
Glu Arg Thr Leu Asp Tyr His Asp Ser Asn Val Lys Asn Leu Tyr Glu 
    450                 455                 460                 
Lys Val Arg Ser Gln Leu Lys Asn Asn Ala Lys Glu Ile Gly Asn Gly 
465                 470                 475                 480 
Cys Phe Glu Phe Tyr His Lys Cys Asp Asn Thr Cys Met Glu Ser Val 
                485                 490                 495     
Lys Asn Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu 
            500                 505                 510         
Asn Arg Glu Glu Ile Asp Gly Val Lys Leu Glu Ser Thr Arg Ile Tyr 
        515                 520                 525             
Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val 
    530                 535                 540                 
Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu 
545                 550                 555                 560 
Gln Cys Arg Ile Cys Ile 
                565     

<210> 13
<211> 1410
<212> DNA
<213> Influenza A virus


<220> 
<223> >KR700793.1 Influenza A virus (A/swine/England/61470/2013(H1N2))
      segment 6 neuraminidase (NA) gene, complete cds

<400> 13
atgaatccaa atcagaagat aataacaatt ggctctgttt ctctcgtcat tgcaacatta      60

tgcttcttaa tgcagatggc catcctaata actactgtaa aattacattt caaacaatat     120

gagtgcggct tccctgcgaa caaccaagta ataacatgtg agccaacagt aatagaaagg     180

aacacaacag agatagtgta cttaactaac accaccatag agaaagaaac atgccacaaa     240

acagtggaat acaggaattg gtcaaagcct caatgcaaaa taacaggctt tgcacctttt     300

tccaaggaca attcaattcg actttctgct ggtggggaca tatgggtgac gagggaacct     360

tacgtgtcat gcgagcctgg caaatgttat cagtttgcac tcgggcaagg gaccacacta     420

gacaataaac attcaaacga tacaatacat gacagaaccc cctatcgaac tctattgatg     480

aatgaattgg gtgtcccatt tcatttaggg acaagacaag tgtgtattgc atggtccagc     540

tcaagttgtt atgatgggaa agcatggttg catgtctgta tcactggaca tgataaaaat     600

gcaactgcca gtttcattta cgatggtaga cttgtagata gcattggttc atggtctaaa     660

aatatactta gaacccagga atcagaatgc gtttgcatca atggggtctg tacagtagta     720

atgactgatg gaagtgcttc gggaagagct gatactaaaa tactattcat tgaagaaggg     780

aaaattgttc atattagccc attagcgggg agtgcacagc atgtggagga gtgctcctgt     840

tatccccgat atcctggcgt aaggtgtatc tgcagagaca actggaaagg ctctaacaga     900

cccgttgtgg atataaatat agaagattat agcattgatt ccagttatgt gtgttcaggg     960

cttgttggcg acacacccag aatcaatgac ggatccagta gtagctactg ccgtgatcct    1020

aacaacgaaa aaggaaatca cggagtgaag ggctgggctt ttgacgatgg aaatgatgtg    1080

tggatgggaa gaacgatcaa cgaagattca cgctcaggtt atgaaacatt caaagtcatt    1140

ggtggttggt ccactcctaa ttccaaattg cagataaata ggcaagtaat agttgatagc    1200

aacaataggt caggttattc tggtgttttc tccgttgaag gcaaaagctg catcaataga    1260

tgtttctacg tggagttgat aagaggaaga aggtcagaag cgcgagtatg gtggacctca    1320

aacagtattg ttgtattttg tggcacttca ggtacctatg gaacaggctc atggcctgat    1380

ggagcagaca tcaacctcat gcctatatga                                     1410


<210> 14
<211> 1410
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUN2-6 Swine optimized sequence

<400> 14
atgaatccga accagaaaat tatcaccatt gggtccgtca gccttgtgat tgccactctg      60

tgctttctga tgcaaatggc catcctcatt accactgtga agctgcattt caagcagtac     120

gaatgcggct tccccgcgaa caaccaggtc atcacttgcg agccaaccgt gattgagcgg     180

aacactaccg aaattgtata cctcacgaac actaccattg aaaaggagac ttgccacaag     240

accgtggaat accggaactg gtccaagcct cagtgcaaga ttaccgggtt cgccccattc     300

tccaaggaca atagcatccg cctttcggcc ggaggagata tttgggtgac gcgcgaaccc     360

tacgtcagct gcgaacccgg gaagtgttac cagttcgcac tgggccaggg aaccaccctg     420

gataataagc acagcaacga caccatccac gaccggactc cctaccgcac cctcctgatg     480

aatgagctcg gcgtgccgtt ccacctgggt accagacagg tctgcatcgc ctggtcgtcc     540

tcctcatgct acgatgggaa ggcttggctc cacgtgtgca taaccggtca tgacaagaac     600

gccacagcct cattcatcta cgacggacgg ctagtggact ccatcggctc ctggtccaag     660

aacattctga ggacccagga atccgaatgc gtctgtatca acggagtgtg caccgtggtg     720

atgaccgatg gctccgcaag cggaagggcc gatactaaga tcctgttcat cgaggaggga     780

aagattgtgc acatctctcc tctcgcggga tcagcccagc acgtggaaga atgttcctgt     840

taccctcgct acccgggagt gcgctgtatt tgccgggaca actggaaagg ctccaacagg     900

cctgtggtgg acattaatat cgaggactac tccatcgata gctcctacgt gtgctccggt     960

ctggtcgggg acactccaag aatcaacgat ggaagctcca gcagctactg ccgggacccc    1020

aataacgaaa aggggaacca cggcgtgaag ggttgggcgt tcgacgatgg aaatgacgtc    1080

tggatgggac ggactatcaa cgaggactca cggagcggat acgaaacctt taaggtcatc    1140

ggcggatggt cgaccccgaa cagcaagctg cagatcaacc gccaagtgat cgtggactcc    1200

aacaaccgct cggggtatag cggggtgttc tccgtggagg gaaagtcctg catcaaccgg    1260

tgcttctacg tcgaactgat cagaggccgg agatcagaag cccgcgtgtg gtggacctcc    1320

aactccattg tggtgttttg cggcacttcc gggacctatg gcactggatc ctggcccgat    1380

ggagcagaca tcaacctgat gccaatctga                                     1410


<210> 15
<211> 469
<212> PRT
<213> Influenza A virus


<220> 
<223> >AKJ82042.1 neuraminidase [Influenza A virus
      (A/swine/England/61470/2013(H1N2))] EUN2-6

<400> 15
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Val 
1               5                   10                  15      
Ile Ala Thr Leu Cys Phe Leu Met Gln Met Ala Ile Leu Ile Thr Thr 
            20                  25                  30          
Val Lys Leu His Phe Lys Gln Tyr Glu Cys Gly Phe Pro Ala Asn Asn 
        35                  40                  45              
Gln Val Ile Thr Cys Glu Pro Thr Val Ile Glu Arg Asn Thr Thr Glu 
    50                  55                  60                  
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Thr Cys His Lys 
65                  70                  75                  80  
Thr Val Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Lys Ile Thr Gly 
                85                  90                  95      
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly 
            100                 105                 110         
Asp Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Glu Pro Gly Lys 
        115                 120                 125             
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asp Asn Lys His 
    130                 135                 140                 
Ser Asn Asp Thr Ile His Asp Arg Thr Pro Tyr Arg Thr Leu Leu Met 
145                 150                 155                 160 
Asn Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile 
                165                 170                 175     
Ala Trp Ser Ser Ser Ser Cys Tyr Asp Gly Lys Ala Trp Leu His Val 
            180                 185                 190         
Cys Ile Thr Gly His Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asp 
        195                 200                 205             
Gly Arg Leu Val Asp Ser Ile Gly Ser Trp Ser Lys Asn Ile Leu Arg 
    210                 215                 220                 
Thr Gln Glu Ser Glu Cys Val Cys Ile Asn Gly Val Cys Thr Val Val 
225                 230                 235                 240 
Met Thr Asp Gly Ser Ala Ser Gly Arg Ala Asp Thr Lys Ile Leu Phe 
                245                 250                 255     
Ile Glu Glu Gly Lys Ile Val His Ile Ser Pro Leu Ala Gly Ser Ala 
            260                 265                 270         
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Gly Val Arg 
        275                 280                 285             
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Val Val Asp 
    290                 295                 300                 
Ile Asn Ile Glu Asp Tyr Ser Ile Asp Ser Ser Tyr Val Cys Ser Gly 
305                 310                 315                 320 
Leu Val Gly Asp Thr Pro Arg Ile Asn Asp Gly Ser Ser Ser Ser Tyr 
                325                 330                 335     
Cys Arg Asp Pro Asn Asn Glu Lys Gly Asn His Gly Val Lys Gly Trp 
            340                 345                 350         
Ala Phe Asp Asp Gly Asn Asp Val Trp Met Gly Arg Thr Ile Asn Glu 
        355                 360                 365             
Asp Ser Arg Ser Gly Tyr Glu Thr Phe Lys Val Ile Gly Gly Trp Ser 
    370                 375                 380                 
Thr Pro Asn Ser Lys Leu Gln Ile Asn Arg Gln Val Ile Val Asp Ser 
385                 390                 395                 400 
Asn Asn Arg Ser Gly Tyr Ser Gly Val Phe Ser Val Glu Gly Lys Ser 
                405                 410                 415     
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Arg Ser 
            420                 425                 430         
Glu Ala Arg Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 
        435                 440                 445             
Thr Ser Gly Thr Tyr Gly Thr Gly Ser Trp Pro Asp Gly Ala Asp Ile 
    450                 455                 460                 
Asn Leu Met Pro Ile 
465                 

<210> 16
<211> 1410
<212> DNA
<213> Influenza A virus


<220> 
<223> >KU323318.1 Influenza A virus (A/swine/Italy/248147-8/2015(H3N2))
      segment 6 neuraminidase (NA) gene, complete cds

<400> 16
atgaatccaa atcaaaagat aataacaatt ggttctgttt ctctcactat tacaacaatg      60

tgcctcttct tgcagattgc catcctagta actactataa cattgcattt caagcaatat     120

gaatgcgatt cccctgcaaa caaccaagta ataccgtgtg aaccaataat aatagaaaaa     180

aacataacaa aaatagtgta tttgaccaat accaccatag agaaagaggt atgcccaaaa     240

ttaggggaat acaggaattg gtcaaaacca caatgcaaga tcacaggatt tgcacctttt     300

tctaaggaca attcaattcg gctctctgcg ggtggggcca tttgggtcac gagagaacct     360

tatgtgtcat gcgaccctaa caagtgttat caatttgcat taggacaggg aaccacatta     420

gataacagac attcaaatga cacaatacat gatagaaccc cttttagaac cctgttgatg     480

agtgaattag gtgttccatt tcatttggga accagacaag tatgcatagc atggtccagt     540

tcaagttgtc acgatgggaa agcttggttg catgtttgtg tcactgggca tgataaaaat     600

gcaactgcta gtttcattta tgacggaaag cttgtagaca gcatcagttc atggtccaaa     660

aacatactcc ggactcagga atcagaatgt gtttgtatcg atggaatctg tacagtggtg     720

atgactgatg gaagtgcttc agggaaagct gatactaaga tactatttat tgaaaaaggg     780

aagatcattc atattagtcc attgttggga agtgctcagc atgtagaaga atgttcctgt     840

taccctagat accctgatgt caggtgtatt tgcagggata actggaaagg ttcaaatagg     900

cccatcgtag acataagaat gaaaaattat agcattggtt ccagttatat gtgctcagga     960

cttgttggcg acacacccag gaacaatgat gggtctagta atagcaattg tcggaatccc    1020

aataatgaaa gaggaaatca tggagtgaaa ggttgggcct ttgatgatgg aaatgacaca    1080

tggatgggaa gaactatcag caaggactca cgcttaggtt acgaaacctt caaagttgtt    1140

ggtggttggt cccaacccaa ttccaaatcc cagataaata gacaagttat tgttgacagc    1200

gataatagat caggttactc tggtattttc tctgttgagg ggaaagattg cattaatagg    1260

tgtttttatg tggaactaat aagaggaagg agacaggaaa ctagagtgtg gtggacttcg    1320

aacagtattg ttgtgttctg tggcacttct ggcacctatg ggtcaggctc atggcccgat    1380

ggagcaaaca tcaatttcat gcctgtataa                                     1410


<210> 17
<211> 1410
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUN2-7 Swine optimized sequence

<400> 17
atgaacccta atcagaagat catcacaatc ggctccgtgt ccctcaccat caccactatg      60

tgcctcttcc ttcaaatcgc cattctggtc accaccatca ccctgcattt caagcagtac     120

gaatgcgact caccagcgaa caatcaggtc atcccgtgtg aaccgatcat tatcgagaag     180

aacatcacta agattgtgta tttgaccaac accaccattg agaaggaagt ctgcccaaag     240

ctcggggaat accgcaactg gagcaagccc cagtgcaaga ttaccggctt cgccccgttc     300

tcaaaagaca actccatccg gctgtctgcg ggaggagcca tctgggtcac gagagaaccg     360

tacgtgtcgt gcgaccccaa caagtgctac cagttcgccc tgggacaagg gaccaccctg     420

gacaaccggc actccaacga cactatacat gataggactc ccttccggac ccttctcatg     480

agcgaactgg gggtcccctt ccatctcgga accagacaag tctgcattgc gtggagctcc     540

tcctcctgcc acgacggaaa agcttggctc cacgtctgcg tgacgggcca cgacaagaac     600

gccactgcct ccttcatcta cgatgggaag cttgtggact ccatctcgtc atggagcaag     660

aacatcctgc ggactcagga gtcagaatgc gtgtgcatcg acggcatttg caccgtcgtg     720

atgactgacg gttcggccag cggaaaggcc gacaccaaga tcctgttcat cgagaaggga     780

aagatcattc acatctcccc acttctgggt tccgcgcaac acgtggaaga atgctcctgc     840

tacccccgct accccgatgt ccggtgcatc tgccgggaca actggaaggg ttccaaccgg     900

ccaatcgtgg acatccgcat gaagaactat tccatcgggt cctcttacat gtgctcgggt     960

ctggtcggag atacaccgcg caacaacgac gggtcctcaa acagcaactg ccggaaccct    1020

aacaacgaga ggggcaacca cggcgtgaaa ggatgggctt tcgacgacgg aaatgacact    1080

tggatgggga gaaccattag caaggatagc aggcttggct acgaaacctt caaggtcgtg    1140

ggcggttggt cgcagccgaa ctcgaagtcg cagatcaaca gacaggtcat cgtggactcg    1200

gataacagat cggggtactc agggatcttc tccgtggagg gaaaggactg catcaataga    1260

tgtttctacg tcgagctgat tcgaggccgg agacaggaaa cccgcgtatg gtggacgtcc    1320

aactcaatcg tcgtgttttg cggtacctcc ggaacttacg gctcgggatc atggcccgat    1380

ggagccaata ttaacttcat gcctgtgtga                                     1410


<210> 18
<211> 469
<212> PRT
<213> Influenza A virus


<220> 
<223> >ALX30429.1 neuraminidase [Influenza A virus
      (A/swine/Italy/248147-8/2015(H3N2))] EUN2-7

<400> 18
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Ser Leu Thr 
1               5                   10                  15      
Ile Thr Thr Met Cys Leu Phe Leu Gln Ile Ala Ile Leu Val Thr Thr 
            20                  25                  30          
Ile Thr Leu His Phe Lys Gln Tyr Glu Cys Asp Ser Pro Ala Asn Asn 
        35                  40                  45              
Gln Val Ile Pro Cys Glu Pro Ile Ile Ile Glu Lys Asn Ile Thr Lys 
    50                  55                  60                  
Ile Val Tyr Leu Thr Asn Thr Thr Ile Glu Lys Glu Val Cys Pro Lys 
65                  70                  75                  80  
Leu Gly Glu Tyr Arg Asn Trp Ser Lys Pro Gln Cys Lys Ile Thr Gly 
                85                  90                  95      
Phe Ala Pro Phe Ser Lys Asp Asn Ser Ile Arg Leu Ser Ala Gly Gly 
            100                 105                 110         
Ala Ile Trp Val Thr Arg Glu Pro Tyr Val Ser Cys Asp Pro Asn Lys 
        115                 120                 125             
Cys Tyr Gln Phe Ala Leu Gly Gln Gly Thr Thr Leu Asp Asn Arg His 
    130                 135                 140                 
Ser Asn Asp Thr Ile His Asp Arg Thr Pro Phe Arg Thr Leu Leu Met 
145                 150                 155                 160 
Ser Glu Leu Gly Val Pro Phe His Leu Gly Thr Arg Gln Val Cys Ile 
                165                 170                 175     
Ala Trp Ser Ser Ser Ser Cys His Asp Gly Lys Ala Trp Leu His Val 
            180                 185                 190         
Cys Val Thr Gly His Asp Lys Asn Ala Thr Ala Ser Phe Ile Tyr Asp 
        195                 200                 205             
Gly Lys Leu Val Asp Ser Ile Ser Ser Trp Ser Lys Asn Ile Leu Arg 
    210                 215                 220                 
Thr Gln Glu Ser Glu Cys Val Cys Ile Asp Gly Ile Cys Thr Val Val 
225                 230                 235                 240 
Met Thr Asp Gly Ser Ala Ser Gly Lys Ala Asp Thr Lys Ile Leu Phe 
                245                 250                 255     
Ile Glu Lys Gly Lys Ile Ile His Ile Ser Pro Leu Leu Gly Ser Ala 
            260                 265                 270         
Gln His Val Glu Glu Cys Ser Cys Tyr Pro Arg Tyr Pro Asp Val Arg 
        275                 280                 285             
Cys Ile Cys Arg Asp Asn Trp Lys Gly Ser Asn Arg Pro Ile Val Asp 
    290                 295                 300                 
Ile Arg Met Lys Asn Tyr Ser Ile Gly Ser Ser Tyr Met Cys Ser Gly 
305                 310                 315                 320 
Leu Val Gly Asp Thr Pro Arg Asn Asn Asp Gly Ser Ser Asn Ser Asn 
                325                 330                 335     
Cys Arg Asn Pro Asn Asn Glu Arg Gly Asn His Gly Val Lys Gly Trp 
            340                 345                 350         
Ala Phe Asp Asp Gly Asn Asp Thr Trp Met Gly Arg Thr Ile Ser Lys 
        355                 360                 365             
Asp Ser Arg Leu Gly Tyr Glu Thr Phe Lys Val Val Gly Gly Trp Ser 
    370                 375                 380                 
Gln Pro Asn Ser Lys Ser Gln Ile Asn Arg Gln Val Ile Val Asp Ser 
385                 390                 395                 400 
Asp Asn Arg Ser Gly Tyr Ser Gly Ile Phe Ser Val Glu Gly Lys Asp 
                405                 410                 415     
Cys Ile Asn Arg Cys Phe Tyr Val Glu Leu Ile Arg Gly Arg Arg Gln 
            420                 425                 430         
Glu Thr Arg Val Trp Trp Thr Ser Asn Ser Ile Val Val Phe Cys Gly 
        435                 440                 445             
Thr Ser Gly Thr Tyr Gly Ser Gly Ser Trp Pro Asp Gly Ala Asn Ile 
    450                 455                 460                 
Asn Phe Met Pro Val 
465                 

<210> 19
<211> 1410
<212> DNA
<213> Influenza A virus


<220> 
<223> >KU323247.1 Influenza A virus (A/swine/Italy/179057/2015(H1N1))
      segment 6 neuraminidase (NA) gene, complete cds

<400> 19
atgaatccaa accaaaagat aataaccatt ggttcggtct gtatgacaat tggaatggct      60

aacttaatat tacaaattgg aaacataatc tcaatatgga ttagccactc aattcaactt     120

gggaatcaaa gtcagattga aacatgcaat caaagcgtca ttacttatga aaacaacact     180

tgggtaaatc agacatatgt taacatcagc aacaccaact ttgctgctgg acagtcagtg     240

gtttccgcga aattagcggg caattcctcc ctctgccctg ttagtggatg ggctatatac     300

agtaaagaca acagtgtaag aatcggttcc aagggggatg tgtttgtcat aagggaacca     360

ttcatatcat gctccccctt agaatgcaga accttcttct tgactcaagg ggccttgcta     420

aatgacaaac attccaatgg aaccattaaa gataggagcc catatcgaac cctgatgagc     480

tgtcctattg gtgaagttcc ctctccatac aactcaagat ttgagtcggt cgcttggtca     540

gcaagtgctt gtcacgatgg catcaattgg ctaacaatcg gaatttctgg cccagacagt     600

ggggcagtgg ctgtattaaa gtacaatggc ataataacag acactatcaa gagttggaaa     660

aacaatatat tgagaacaca agagtctgaa tgtgcatgtg taaatggttc ttgctttacc     720

ataatgaccg atggaccaag tgatggacag gcctcataca agatcttcag aatagaaaag     780

ggaaagatag tcaaatcagt cgaaatgaat gcccctaatt atcactatga ggaatgctcc     840

tgttatcctg attctagtga aatcacatgt gtgtgcaggg ataactggca tggctcgaat     900

cgaccgtggg tgtctttcaa ccagaatctg gaatatcaga taggatacat atgcagtggg     960

attttcggag acaatccacg ccctaatgat aagacaggca gttgtggtcc agtatcgtct    1020

aatggagcaa atggagtaaa aggattttca ttcaaatatg gcaatggtgt ttggataggg    1080

agaactaaaa gcattagttc aagaaaaggt tttgagatga tttgggatcc aaatggatgg    1140

actgggacag acaaaaactt ctcaataaag caagatatca taggaataaa tgagtggtca    1200

ggatacagcg ggagttttgt tcagcatcca gaactaacag ggctgaattg tataagacct    1260

tgcttctggg ttgaactaat cagagggcga cccaaagaga acacaatctg gactagcggg    1320

agcagcatat ccttttgtgg tgtaaacagt gacactgtgg gttggtcttg gccagacggt    1380

gctgagttgc catttaccat tgacaagtaa                                     1410


<210> 20
<211> 1410
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUN1-4 Swine optimized sequence

<400> 20
atgaacccta accagaagat tattaccatc ggatccgtgt gcatgaccat cggaatggcc      60

aacctgatcc tgcaaatcgg aaacatcatc tccatctgga ttagccatag cattcagctt     120

ggaaaccaga gccagatcga gacttgtaac cagtccgtga tcacctacga gaacaacacc     180

tgggtcaacc agacttacgt caatatcagc aacaccaact tcgctgcggg gcagtccgtg     240

gtgtccgcaa agctggccgg caacagctcc ctgtgccccg tgtccggatg ggcgatctac     300

tccaaggata acagcgtgcg catcgggtcc aaaggggacg tgttcgtcat ccgcgaacca     360

ttcatttctt gctccccctt ggaatgtcgg accttcttcc tgacccaagg ggcgttgctc     420

aacgacaagc acagcaacgg aaccatcaaa gatcggtcgc cctaccgcac tctgatgtcg     480

tgccctatcg gcgaagtgcc atccccctac aactcacgct tcgagtccgt ggcctggtcc     540

gcttccgcct gccacgatgg aatcaactgg ctcacaatcg gcatctccgg cccggactcg     600

ggagccgtgg ccgtgctgaa gtacaatggt attattactg acactatcaa gtcgtggaag     660

aacaatattc tccggactca agaatctgaa tgcgcctgcg tgaacggttc ctgcttcact     720

atcatgaccg acggcccttc cgatggacag gcctcataca agatcttccg gatcgagaag     780

ggaaagatcg tgaagtccgt cgagatgaac gcaccgaact accattatga ggaatgctcg     840

tgctacccgg actcctcgga aattacttgc gtgtgccgcg acaattggca cgggtccaac     900

aggccctggg tgtccttcaa ccaaaacctg gagtaccaga tcggttacat ctgctccggg     960

atttttggag acaaccctag acctaacgac aagaccggct catgcggacc tgtgtcctcc    1020

aacggagcca acggcgtgaa gggattctcg ttcaaatatg ggaacggcgt ctggataggt    1080

cggaccaagt ccatctcgtc acggaagggc tttgaaatga tttgggaccc gaacggttgg    1140

accggaaccg acaagaactt cagcatcaag caggacatta tcggcattaa cgagtggagc    1200

ggatactcgg gcagcttcgt ccagcacccg gaacttacgg gcctcaattg tattaggccc    1260

tgtttttggg tcgagctgat tagagggcgc cccaaggaaa acaccatctg gaccagcggc    1320

tccagcatct cattctgcgg agtgaactcc gacaccgtgg gctggtcgtg gcccgacggt    1380

gccgagctgc cgttcaccat cgataaatga                                     1410


<210> 21
<211> 469
<212> PRT
<213> Influenza A virus


<220> 
<223> >ALX30323.1 neuraminidase [Influenza A virus
      (A/swine/Italy/179057/2015(H1N1))] EUN1-4

<400> 21
Met Asn Pro Asn Gln Lys Ile Ile Thr Ile Gly Ser Val Cys Met Thr 
1               5                   10                  15      
Ile Gly Met Ala Asn Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile 
            20                  25                  30          
Trp Ile Ser His Ser Ile Gln Leu Gly Asn Gln Ser Gln Ile Glu Thr 
        35                  40                  45              
Cys Asn Gln Ser Val Ile Thr Tyr Glu Asn Asn Thr Trp Val Asn Gln 
    50                  55                  60                  
Thr Tyr Val Asn Ile Ser Asn Thr Asn Phe Ala Ala Gly Gln Ser Val 
65                  70                  75                  80  
Val Ser Ala Lys Leu Ala Gly Asn Ser Ser Leu Cys Pro Val Ser Gly 
                85                  90                  95      
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly 
            100                 105                 110         
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser Pro Leu Glu 
        115                 120                 125             
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His 
    130                 135                 140                 
Ser Asn Gly Thr Ile Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser 
145                 150                 155                 160 
Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Arg Phe Glu Ser 
                165                 170                 175     
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Ile Asn Trp Leu Thr 
            180                 185                 190         
Ile Gly Ile Ser Gly Pro Asp Ser Gly Ala Val Ala Val Leu Lys Tyr 
        195                 200                 205             
Asn Gly Ile Ile Thr Asp Thr Ile Lys Ser Trp Lys Asn Asn Ile Leu 
    210                 215                 220                 
Arg Thr Gln Glu Ser Glu Cys Ala Cys Val Asn Gly Ser Cys Phe Thr 
225                 230                 235                 240 
Ile Met Thr Asp Gly Pro Ser Asp Gly Gln Ala Ser Tyr Lys Ile Phe 
                245                 250                 255     
Arg Ile Glu Lys Gly Lys Ile Val Lys Ser Val Glu Met Asn Ala Pro 
            260                 265                 270         
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Ser Glu Ile 
        275                 280                 285             
Thr Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val 
    290                 295                 300                 
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly 
305                 310                 315                 320 
Ile Phe Gly Asp Asn Pro Arg Pro Asn Asp Lys Thr Gly Ser Cys Gly 
                325                 330                 335     
Pro Val Ser Ser Asn Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Lys 
            340                 345                 350         
Tyr Gly Asn Gly Val Trp Ile Gly Arg Thr Lys Ser Ile Ser Ser Arg 
        355                 360                 365             
Lys Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp 
    370                 375                 380                 
Lys Asn Phe Ser Ile Lys Gln Asp Ile Ile Gly Ile Asn Glu Trp Ser 
385                 390                 395                 400 
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asn 
                405                 410                 415     
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys 
            420                 425                 430         
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val 
        435                 440                 445             
Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro 
    450                 455                 460                 
Phe Thr Ile Asp Lys 
465                 

<210> 22
<211> 1410
<212> DNA
<213> Influenza A virus


<220> 
<223> >KR700532.1 Influenza A virus (A/swine/Italy/28762-3/2013(H1N1))
      segment 6 neuraminidase (NA) gene, complete cds

<400> 22
atgaacccaa atcagaagat aataatcatt agttcaatct gtatgacaaa tggaattgct      60

agcttgatat tacaaattgg gaacataata tcaatatgga ttagccattc aattcaaatt     120

gagaacccaa accagaccga accatgcaat caaagcgtta ttatttacga aaacaacaca     180

tgggtaaatc aaacgtatgt taacatcagc aacaataatt ttgttgttga acagacagtg     240

gtttcaatga aattagcggg cagttcttct ctctgccctg ttagtggatg ggctatatac     300

agtaaagata acagtgtaag aatcggttcc aaaggggatg tgtttgtcat aagagagcca     360

ttcatctcat gctcccattt ggaatgtaga accttcttct taactcaagg ggccctactg     420

aatgataaac attctaatgg aaccgttaaa gacagaagcc cctatcgaac cctgatgagc     480

tgtcctattg gtgaagtccc ctctccatac aactcaaaat ttgagtcagt tgcttggtca     540

gcaagtgctt gccatgatgg caccagttgg ttgacaattg ggatttctgg tccagacaat     600

ggagcagtgg ctgtgttgaa atacaatgac ataataacag acactatcaa gagttggaaa     660

aacaacatat tgagaacaca agaatctgaa tgtgcatgtt tgaatggttc ttgctttact     720

gtaatgaccg atggaccaag taatgggcag gcctcataca agatcttcaa aatagaaaag     780

gggaaagtag tcaaatcagt cgagttgaat gctcctaatt atcactatga ggaatgttcc     840

tgttatcctg attctggtga aatcatatgt gtatgcaggg acaattggca tggctcgaat     900

cgaccatggg tgtctttcaa tcagaatctg gagtatcaga taggatacat atgcagtggg     960

gttctcggag acaatccgcg ccctaatgat agaacaggca gttgtggtcc agtatcatct    1020

catggagcaa atggggtaaa agggttttcg tttaaatacg gcaatggaat ttggataggg    1080

agaactaaaa gcactattac aaggagtggt tttgagatga tttgggaccc aaacggatgg    1140

actggaacag acaataattt ctcagtgaag caagatatcg taggaataac taactggtca    1200

ggatacagcg ggagttttgt ccaacatcca gaattaaccg gattggattg tattagacct    1260

tgcttctggg ttgaactaat cagagggaga cccaaagaga acacaatctg gactagcgga    1320

agcagcatat ccttttgtgg tgtaaatagt gacactgtgg gttggtcttg gccagacggt    1380

gctgagttgc catttaccat tgacaagtaa                                     1410


<210> 23
<211> 1410
<212> DNA
<213> Artificial Sequence


<220> 
<223> >EUN1-2 Swine optimized sequence

<400> 23
atgaatccga atcagaagat cattatcatc tcctccattt gtatgactaa cggaatcgca      60

tctctgatct tgcaaattgg gaacatcatt agcatctgga tctcccactc gatccaaatc     120

gagaacccga accagaccga gccctgcaac cagagcgtga tcatatacga aaacaatacc     180

tgggtgaacc agacctatgt caatatctcg aacaacaact tcgtggtgga gcagactgtc     240

gtgtccatga agctcgccgg gtccagctcg ttgtgccctg tctccggctg ggctatctac     300

agcaaagaca acagcgtcag aatcggcagc aagggagatg tgttcgtgat ccgcgagcct     360

ttcatctcct gctcacacct ggaatgccgg acctttttcc tgacacaagg cgccctgctc     420

aatgacaagc actcgaacgg gaccgtgaag gacagaagcc cctacagaac cctgatgtcc     480

tgtccgattg gagaagtgcc ctccccgtat aactcaaagt tcgaatccgt ggcttggtca     540

gcatccgcgt gccatgacgg gaccagctgg ctgactatcg gaatctccgg tcctgacaac     600

ggcgcggtcg ccgtgctcaa gtacaacgac attatcactg acaccatcaa gtcctggaaa     660

aacaacatcc tccggaccca agagtccgag tgcgcctgcc tgaacggttc gtgtttcacc     720

gtgatgaccg atggaccaag caacggacaa gcctcgtaca agattttcaa gatcgaaaag     780

ggaaaagtgg tcaagtccgt ggagctgaac gcccctaact accactacga agagtgttca     840

tgctaccctg actccggcga aattatctgc gtgtgtcggg ataactggca cggatccaac     900

cgcccctggg tcagcttcaa ccagaacctg gaatatcaga tcggctacat ttgctccggc     960

gtgctggggg acaacccgag gccgaatgac cgcactggtt cctgcggacc cgtgtcatcc    1020

cacggggcga acggagtgaa gggtttctct ttcaaatacg gaaacggcat ctggattggc    1080

agaaccaaga gcactatcac ccggagcgga tttgagatga tctgggaccc caacggatgg    1140

accggtaccg ataacaactt ttcggtcaag caggatattg tgggcatcac taactggtcg    1200

ggatactccg gctcgttcgt gcagcatccc gagctgactg gcctggactg cattcgcccg    1260

tgcttctggg tggaacttat caggggtcgg cctaaggaga acactatctg gacctccgga    1320

tcgtcgattt ccttctgcgg cgtgaactca gataccgtgg gatggtcctg gccggacggg    1380

gccgagcttc cattcacgat tgataagtga                                     1410


<210> 24
<211> 469
<212> PRT
<213> Influenza A virus


<220> 
<223> >AKJ81669.1 neuraminidase [Influenza A virus
      (A/swine/Italy/28762-3/2013(H1N1))] EUN1-2

<400> 24
Met Asn Pro Asn Gln Lys Ile Ile Ile Ile Ser Ser Ile Cys Met Thr 
1               5                   10                  15      
Asn Gly Ile Ala Ser Leu Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile 
            20                  25                  30          
Trp Ile Ser His Ser Ile Gln Ile Glu Asn Pro Asn Gln Thr Glu Pro 
        35                  40                  45              
Cys Asn Gln Ser Val Ile Ile Tyr Glu Asn Asn Thr Trp Val Asn Gln 
    50                  55                  60                  
Thr Tyr Val Asn Ile Ser Asn Asn Asn Phe Val Val Glu Gln Thr Val 
65                  70                  75                  80  
Val Ser Met Lys Leu Ala Gly Ser Ser Ser Leu Cys Pro Val Ser Gly 
                85                  90                  95      
Trp Ala Ile Tyr Ser Lys Asp Asn Ser Val Arg Ile Gly Ser Lys Gly 
            100                 105                 110         
Asp Val Phe Val Ile Arg Glu Pro Phe Ile Ser Cys Ser His Leu Glu 
        115                 120                 125             
Cys Arg Thr Phe Phe Leu Thr Gln Gly Ala Leu Leu Asn Asp Lys His 
    130                 135                 140                 
Ser Asn Gly Thr Val Lys Asp Arg Ser Pro Tyr Arg Thr Leu Met Ser 
145                 150                 155                 160 
Cys Pro Ile Gly Glu Val Pro Ser Pro Tyr Asn Ser Lys Phe Glu Ser 
                165                 170                 175     
Val Ala Trp Ser Ala Ser Ala Cys His Asp Gly Thr Ser Trp Leu Thr 
            180                 185                 190         
Ile Gly Ile Ser Gly Pro Asp Asn Gly Ala Val Ala Val Leu Lys Tyr 
        195                 200                 205             
Asn Asp Ile Ile Thr Asp Thr Ile Lys Ser Trp Lys Asn Asn Ile Leu 
    210                 215                 220                 
Arg Thr Gln Glu Ser Glu Cys Ala Cys Leu Asn Gly Ser Cys Phe Thr 
225                 230                 235                 240 
Val Met Thr Asp Gly Pro Ser Asn Gly Gln Ala Ser Tyr Lys Ile Phe 
                245                 250                 255     
Lys Ile Glu Lys Gly Lys Val Val Lys Ser Val Glu Leu Asn Ala Pro 
            260                 265                 270         
Asn Tyr His Tyr Glu Glu Cys Ser Cys Tyr Pro Asp Ser Gly Glu Ile 
        275                 280                 285             
Ile Cys Val Cys Arg Asp Asn Trp His Gly Ser Asn Arg Pro Trp Val 
    290                 295                 300                 
Ser Phe Asn Gln Asn Leu Glu Tyr Gln Ile Gly Tyr Ile Cys Ser Gly 
305                 310                 315                 320 
Val Leu Gly Asp Asn Pro Arg Pro Asn Asp Arg Thr Gly Ser Cys Gly 
                325                 330                 335     
Pro Val Ser Ser His Gly Ala Asn Gly Val Lys Gly Phe Ser Phe Lys 
            340                 345                 350         
Tyr Gly Asn Gly Ile Trp Ile Gly Arg Thr Lys Ser Thr Ile Thr Arg 
        355                 360                 365             
Ser Gly Phe Glu Met Ile Trp Asp Pro Asn Gly Trp Thr Gly Thr Asp 
    370                 375                 380                 
Asn Asn Phe Ser Val Lys Gln Asp Ile Val Gly Ile Thr Asn Trp Ser 
385                 390                 395                 400 
Gly Tyr Ser Gly Ser Phe Val Gln His Pro Glu Leu Thr Gly Leu Asp 
                405                 410                 415     
Cys Ile Arg Pro Cys Phe Trp Val Glu Leu Ile Arg Gly Arg Pro Lys 
            420                 425                 430         
Glu Asn Thr Ile Trp Thr Ser Gly Ser Ser Ile Ser Phe Cys Gly Val 
        435                 440                 445             
Asn Ser Asp Thr Val Gly Trp Ser Trp Pro Asp Gly Ala Glu Leu Pro 
    450                 455                 460                 
Phe Thr Ile Asp Lys 
465                 

<210> 25
<211> 13
<212> DNA
<213> Artificial Sequence


<220> 
<223> 5` flanking sequence of open reading frame

<400> 25
ggcgcgccgc acc                                                       13


