                         SEQUENCE LISTING

<110>  BASF

<120>  Plasmid copy number regulation and integration

<130>  PF 191586

<160>  84

<170>  According Wipo Std 25



<210> 1
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pE194

<400> 1
tatatacagc tggattcaca aaaaataggc ac                                   32

<210> 2
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pE194

<400> 2
tatatacagc tggattatgt cttttgcgca gtc                                  33

<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of functional mRFP cass
      ette

<400> 3
tatatggatc cgtaatcagg gtatcgaggc                                      30

<210> 4
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of functional mRFP cass
      ette

<400> 4
tatatggatc cctcattagg cgggctacta a                                    31

<210> 5
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 5
tataggtctc aacccataat gccgtcgcac tgg                                  33

<210> 6
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 6
ccttggtctc gtcgctagaa gagcagagag gacgg                                35

<210> 7
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 7
ttgagaggtc tcagagacat tttccctata ttttcttcc                            39

<210> 8
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 8
gtgaggtctc atgagaccat ccgttattga caagg                                35

<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 9
ttgcccgaat acaacgacag                                                 20

<210> 10
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of amyB genomic region

<400> 10
caacaacagg ctgtctgacg g                                               21

<210> 11
<211> 2143
<212> DNA
<213> Bacillus licheniforms

<220>
<223> P300 DSM641 RMS: RMS region and flanking region

<400> 11
aacttctata aatgtaacga tacgatttat tgtttcatta aagtcttcct ttatttcatg     60
ttcccatatt cttttaatgt tccaatcctt ttcctcgtaa tatttattaa cttccttatc    120
tcttttttta tttctttcga gttttttctc ccaatattcc gtattacttt ttggtatatt    180
cccgtgtttt tcacacgcat gccagaaaca agaatcaatg aatatgacta ttttatattt    240
ctgtattact atatctggac taccgtataa tttcttaaca ttttttcgga atcttattcc    300
acggtgccat agttctttag taaccttatc ttctaatttt gaacgagatt tgattgcctg    360
catgtttttt cttctttgtt cttttgaaac cgtgtcagtc atagaagagt cctccaaagc    420
cacaataatt gtattctata aacgaggaag caagccctca agcttacccc ctcttagttc    480
cttttttgcc tacttattta tttgttttca ttttcaaatt catcataaga acccatttca    540
caaaaatcaa tggagtaatg ttgttcgctg tgtttataaa caattactga gtcaatattt    600
agtctttcca gcatttcata ggttagaagt tctttttcct ctaagttcat aacttgtctt    660
agtagccatt ctccaagagc actatttgga ttagacataa gtgctttact attgtcttgg    720
cataccttgg ctgataaaag cgatttgtct ggcaagcgta actgaaaagg tttatcacga    780
gctgggaaaa atgttgggaa tacattatga atccattttg gaattggtat ataaatctcg    840
ttagggtttc gtggtcgacc taaagcattc cattggttta gaccgctttt ttctggtaca    900
tgacgctttg agccacggtc tgaaaagagt ggaagaataa cgtgctcaag gttttcaaaa    960
ggattgacag ttggtgctgg aatttttgga atttcaaagc caaatagttt agccaattca   1020
tgataaggat tttctaagat ttcaacatta atttcttcaa taggtttatc agtgataaaa   1080
cgcttataaa gggtgctctt agtgacatta aagctgtatt cgtgtagacc gtcttcaaag   1140
gtgattgtat ttctgttgtt acttactttc acatttgtaa ttgaggagat ttcaaccaag   1200
tccattggct cttcaaaaat aagaattttc cctggctttc ttgttacaca gtggtatatc   1260
attgaatcaa taccatatgt tcttttagta aattcaattc tctcgttacg gagagaagca   1320
accgtgttta ttagctcttt tggagatttc ccacgataca agtctgagtc tttattgaat   1380
tcagctactt tttgaagagt atgaccatta ccatgaagaa aagtcttaat accaattccg   1440
acacgattta atgaagcgtc agcagaacag tctgacctcc ccaagttttc agctccaaat   1500
gcttcacaaa aagcattttc cacattcctt gagaccaaat aaggcgagtc actttcagag   1560
aacaaattgg atagcgaacc agttgagcgg agcatttgtt tgtatgtagt gcagttgatg   1620
gctggttgat tagtatagaa cattattttt cctcctcttt tatgcttgtc atttcttctt   1680
tcagacccaa aaggtagtca gctgatacgt tcaatgtttc agctattctt ttgaaagtgt   1740
ccaatgatgg agttctattt tcactttcat atagtgacca agtgcttcta gtgaccccga   1800
ctttttcagc gatttggctg ggtaataacc tacgagcttc tcttgcattt tgaatacgat   1860
ttccaaggaa aggtatcatt tttgcacctc caagatttgt tgttttcaga gtatcaccag   1920
aacccccgaa aatagtccaa agttagctaa cagcaaacaa ataaaaataa ataagttgtt   1980
tactcttagc aaacttgtta ctaaaatttg ataaagttat tcatttaatc cagctcttat   2040
gctaaaattg cattagcgga caagcttaat gtttgcaagg aggtataatt ttgacttatc   2100
gagtaggtag tatgtttgct gggataggtg gaacttgttt agg                     2143

<210> 12
<211> 1146
<212> DNA
<213> Bacillus licheniforms

<220>
<223> CDS: Restrictase P300 DSM641

<400> 12
ttatttgttt tcattttcaa attcatcata agaacccatt tcacaaaaat caatggagta     60
atgttgttcg ctgtgtttat aaacaattac tgagtcaata tttagtcttt ccagcatttc    120
ataggttaga agttcttttt cctctaagtt cataacttgt cttagtagcc attctccaag    180
agcactattt ggattagaca taagtgcttt actattgtct tggcatacct tggctgataa    240
aagcgatttg tctggcaagc gtaactgaaa aggtttatca cgagctggga aaaatgttgg    300
gaatacatta tgaatccatt ttggaattgg tatataaatc tcgttagggt ttcgtggtcg    360
acctaaagca ttccattggt ttagaccgct tttttctggt acatgacgct ttgagccacg    420
gtctgaaaag agtggaagaa taacgtgctc aaggttttca aaaggattga cagttggtgc    480
tggaattttt ggaatttcaa agccaaatag tttagccaat tcatgataag gattttctaa    540
gatttcaaca ttaatttctt caataggttt atcagtgata aaacgcttat aaagggtgct    600
cttagtgaca ttaaagctgt attcgtgtag accgtcttca aaggtgattg tatttctgtt    660
gttacttact ttcacatttg taattgagga gatttcaacc aagtccattg gctcttcaaa    720
aataagaatt ttccctggct ttcttgttac acagtggtat atcattgaat caataccata    780
tgttctttta gtaaattcaa ttctctcgtt acggagagaa gcaaccgtgt ttattagctc    840
ttttggagat ttcccacgat acaagtctga gtctttattg aattcagcta ctttttgaag    900
agtatgacca ttaccatgaa gaaaagtctt aataccaatt ccgacacgat ttaatgaagc    960
gtcagcagaa cagtctgacc tccccaagtt ttcagctcca aatgcttcac aaaaagcatt   1020
ttccacattc cttgagacca aataaggcga gtcactttca gagaacaaat tggatagcga   1080
accagttgag cggagcattt gtttgtatgt agtgcagttg atggctggtt gattagtata   1140
gaacat                                                              1146

<210> 13
<211> 1022
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5-prime and 3-prime regions of
       restrictase gene with flanking BsaI sites

<400> 13
ggtctcgacc caacttctat aaatgtaacg atacgattta ttgtttcatt aaagtcttcc     60
tttatttcat gttcccatat tcttttaatg ttccaatcct tttcctcgta atatttatta    120
acttccttat ctcttttttt atttctttcg agttttttct cccaatattc cgtattactt    180
tttggtatat tcccgtgttt ttcacacgca tgccagaaac aagaatcaat gaatatgact    240
attttatatt tctgtattac tatatctgga ctaccgtata atttcttaac attttttcgg    300
aatcttattc cacggtgcca tagttcttta gtaaccttat cttctaattt tgaacgagat    360
ttgattgcct gcatgttttt tcttctttgt tcttttgaaa ccgtgtcagt catagaagag    420
tcctccaaag ccacaataat tgtattctat aaacgaggaa gcaagccctc aagcttaccc    480
cctcttagtt ccttttttgc ctacttattt atatttttcc tcctctttta tgcttgtcat    540
ttcttctttc agacccaaaa ggtagtcagc tgatacgttc aatgtttcag ctattctttt    600
gaaagtgtcc aatgatggag ttctattttc actttcatat agtgaccaag tgcttctagt    660
gaccccgact ttttcagcga tttggctggg taataaccta cgagcttctc ttgcattttg    720
aatacgattt ccaaggaaag gtatcatttt tgcacctcca agatttgttg ttttcagagt    780
atcaccagaa cccccgaaaa tagtccaaag ttagctaaca gcaaacaaat aaaaataaat    840
aagttgttta ctcttagcaa acttgttact aaaatttgat aaagttattc atttaatcca    900
gctcttatgc taaaattgca ttagcggaca agcttaatgt ttgcaaggag gtataatttt    960
gacttatcga gtaggtagta tgtttgctgg gataggtgga acttgtttag gctcaggaga   1020
cc                                                                  1022

<210> 14
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of restrictase genomic
      region

<400> 14
gacaatcccc ttttactgac c                                               21

<210> 15
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of restrictase genomic
      region

<400> 15
ctatttcatt tgcccagaca atcc                                            24

<210> 16
<211> 1363
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5-prime and 3-prime regions of
       pga genes with flanking BsaI sites

<400> 16
ggtctcgacc cgaacactga aattttagac cgggctggga tatcgagaca tatatagggg     60
cgtttaatgg cgaatacagt aacaatgaga atagtaagaa aaattaaaaa tgttaaagtt    120
tgatgaatta tcattgaaaa aaattaatgg ctttttaaat cctaggattt taacctaaaa    180
tctgaagaaa taaggtggat cgaacgactc acaaaatatt tggatttgtc aatgaatccc    240
gctttatgct aaaagagatt ttcatttttt gatagatggt ctgattgtca taggacggat    300
ttgttttgaa gagggaacat tggtgacttt ttaacctgtt cgaaaagagc gaaaatacta    360
aaagaaaaga gacatcccgg ctgacagccc atttaaaggg gattgcggcc gggggaaaaa    420
agagatcctg aatccatcct tcaacctttc atctgaaata gggagaaaag tacaaaaatc    480
ataatgtcga attttgaaag cgcatactta aaacgctgac aaaaatctga taggaattaa    540
gaactttcga tttccaaaaa tatcaataaa aagataggca ttaatgactc gggcgaggtg    600
atctttgtca cggaaaattt cgtcgtcttc tgttacataa tgccgattgt gatttcatag    660
tgaaccctga tcccggttat aaaagacctg tgaaaagcgg ccggtttgaa agggaaacac    720
gacaattttc ttaaccggtc agtgtataaa gttttataga aaatcaggag gatatataca    780
tggttttggg gttcatgttt attgtattct tttgaaggga ataaaaactg acaaatttcg    840
actgaagcaa aatttgaaaa tgcatcacct taccaattcg ggatgggaac cgcacctcat    900
gttcatgacc tctttagaat atttcccttc atctttttaa tccgcgctta ggtgaaaaag    960
ctgatcatgc tgtgctgagc gtttcttctc gctatgacgc tgctgtacat gcaaaaaaag   1020
tcctttaaat atcccagttg aatgacgatg aaagaggaaa gaagaggagg aacagatcaa   1080
ttgataaaaa aagcggcaaa caaaaagttg gttttgtttt gtggaattgc ggtgctttgg   1140
atgtctttat ttttaacgaa tcataatgat gtacgcgccg atacgatcgg cgagaaaata   1200
gcggaaactg ccagacagct tgagggtgcg aaatacagct acggcggaga gaagccgaaa   1260
acggggtttg actcgtcagg ctttgtgcaa tatgtgtttc aatcgctcga tattacgctt   1320
ccgagaacgg taaaggaaca atcgactctt ggctcaggag acc                     1363

<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pga genomic region

<400> 17
aaagccttct cctctctatt                                                 20

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of pga genomic region

<400> 18
ttcttgaaaa agacaaggtc                                                 20

<210> 19
<211> 1027
<212> DNA
<213> Artificial Sequence

<220>
<223> homology regions: Fusion of 5-prime and 3-prime regions of
       the aprE gene with flanking BsaI sites

<400> 19
ggtctcgacc cgaagttctt ttttaacata taggtaaaac aatacgaaaa aaggcgccaa     60
gtattgaaga attgcagcag ccgcggcatt tcccttttcg attgaagcaa aaaacgtata    120
ttgaacagta agcattccaa aaatggaaaa tactaaaatc gaacaaatat ctgttttttt    180
cttccatatc tgacacacat gttgaaaacc gtttttcatt gaaacatata acaagagaat    240
gactcccgat gccagaagcc tgacagagac aagcgagccg gcttcaaccg ctcccctttc    300
aaatatgtac tgtgcagcgc ttcccgataa tccccacaat gaagcccctg caagcaccat    360
caatacgcct ttcacatgag ctgatttcat atctttcacc cgtttctgta tgcgatatat    420
tgcatatttt aatagatgat cgacaaggcc gcaacctcct tcggcaaaaa atgatctcat    480
aaaataaatg aatagtattt tcataaaatg agctcaataa catattctaa caaatagcat    540
atagaaaaag ctagtgtttt tagcactagc tttttcttca ttctgatgaa ggttgttcaa    600
tattttgaat ccgttccatg atcgtcggat ggccgtattt aaaaatcttg acgagaaacg    660
gcgggtttgc ctcgctcagc ccggcttttg agagctcttg aaacgtcgaa accgctgcat    720
cgctgttttg cgtcagttca atcgcatact ggtcagcagc tttttcctga tgcctcgaaa    780
ctgcgttcgt aaatggagac gacgcgaaag agatgacccc catcagcatc agaagaagcg    840
gaagtgcggc tagatcggat tttcctgcaa tatgaaggct tcttccatag cggccgatga    900
tccgcttgta cagcttgtcg atcacataaa agacagcaag ggataaaagc agatacccgc    960
caagtcctat gtaaacatgc ttcatcacat agtgccccat ttcgtgcgcc atgatgctca   1020
ggagacc                                                             1027

<210> 20
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of aprE genomic region

<400> 20
ccggttgtca ttgatccttt a                                               21

<210> 21
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification of aprE genomic region

<400> 21
atcctcctgc aaaaaccgta t                                               21

<210> 22
<211> 1500
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: PxylA promoter and xylR repressor Bac
      illus megaterium flanked by BpiI sites

<400> 22
gaagaccttc gagttataga aaatcaatta attgaaagta gctccttcat tcttaagatc     60
aacgtgatat aggtttgcta acctttgcgt tcacttaact aacttatagg ggtaacactt    120
aaaaaagaat caataacgat agaaaccgct cctaaagcag gtgcattttt tcctaacgaa    180
gaaggcaata gttcacattt attgtctaaa tgagaatgga ctctagaaga aacttcgttt    240
ttaatcgtat ttaaaacaat gggatgagat tcaattatat gatttctcaa gataacagct    300
tctatatcaa atgtattaag gatattggtt aatccaattc cgatataaaa gccaaagttt    360
tgaagtgcat ttaacatttc tacatcattt ttatttgcgc gttccacaat ctcttttcga    420
gaaatattct tttcttcttt agagagcgaa gccagtaacg ctttttcaga agcatataat    480
tcccaacagc ctcgatttcc acagctgcat ttgggtccat taaaatctat cgtcatatga    540
cccatttccc cagaaaaacc ctgaacacct ttatacaatt cgttgttaat aacaagtcca    600
gttccaattc cgatattaat actgatgtaa acgatgtttt catagttttt tgtcatacca    660
aatacttttt caccgtatgc tcctgcatta gcttcatttt caacaaaaac cggaacatta    720
aactcactct caattaaaaa ctgcaaatct ttgatattcc aatttaagtt aggcatgaaa    780
ataatttgct gatgacgatc tacaaggcct ggaacacaaa ttcctattcc gactagacca    840
taaggggact caggcatatg ggttacaaaa ccatgaataa gtgcaaataa aatctctttt    900
acttcactag cggaagaact agacaagtca gaagttttct cgagaataat atttccttct    960
aagtcggtta gaattccgtt aagatagtcg actcctatat caataccaat cgagtagcct   1020
gcattcttat taaaaacaag cattacaggc ctcctgccgc ctctagattg ccctgcccca   1080
atttcaaaaa taaaatcttt ttcaagcagt gtatttactt gagaggagac agtagacttg   1140
tttaatcctg taatctcaga gagagttgcc ctggagacag gggagttctt caaaatttca   1200
tctaatatta atttttgatt catttttttt actaaagctt gatctgcaat ttgaataata   1260
accactcctt tgtttatcca ccgaactaag ttggtgtttt ttgaagcttg aattagatat   1320
ttaaaagtat catatctaat attataacta aattttctaa aaaaaacatt gaaataaaca   1380
tttattttgt atatgatgag ataaagttag tttattggat aaacaaacta actcaattaa   1440
gatagttgat ggataaactt gttcacttaa atcaaagggg gaaatgtaat ataagtcttc   1500

<210> 23
<211> 813
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: Cargo vector for type-II-assembly

<400> 23
ttcacctgcg atggcgagaa cttgcgaggt ctcagcgatc gtctcagcga cccgggtcag     60
ctgggtgctt tttttattcc ttcatcagaa tgaagaaaaa gctagtgcta aaaacactag    120
ctttttctat atgctatttg tggcgcgccg gctagggtct tcttaattaa ctcgaggtcg    180
acaagcttac tatgctacat cagagcagat tgtactgaga gtgcaccaga tgcggtgtga    240
aataccgcac agatgcgtaa ggagaaaata ccgcatcaga cgccattcgc cattcaggct    300
acgcaactgt tgggaagggc gatctgtgcg ggcctcttcg ctattacgcc aactggcgaa    360
agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg    420
ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctggaactcc agcttttgtt    480
ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt    540
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag    600
cctggggtgc ctaatgagta agctaactca cattaattgc gttgcgctga tatcgaattc    660
caccgcggtg agctctgtgg catcaaataa aacgaaaagc tcagtcgaaa gactgggcct    720
ttcgttttat ctgttgttgg atccaactag tgaagaccct cgacaatttg agatgagacc    780
ttgcaggtga gatgagacgg tccttaacat cgc                                 813

<210> 24
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR terminator Bsub fwd

<400> 24
caagaagctc atgcaaagac c                                               21

<210> 25
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR terminator Bsub rev

<400> 25
cgatgacaga gcaagagaga c                                               21

<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR plasmid AmpR fwd

<400> 26
atcagcaata aaccagccag                                                 20

<210> 27
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR plasmid AmpR rev

<400> 27
agccatacca aacgacgag                                                  19

<210> 28
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: delta cop+repF fwd

<400> 28
atcgccatgg gacaatggac acctaggg                                        28

<210> 29
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: delta repF rev

<400> 29
atcgccatgg ctcggcgtat gttattcaag                                      30

<210> 30
<211> 43
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PsrfAA(trunc) fwd

<400> 30
gcattctaga gaagacaaat atttttatct ttctaccgtt cag                       43

<210> 31
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PsrfAA(trunc) rev

<400> 31
gcatgaattc gaagacgctc gattagtgga aatgattgcg g                         41

<210> 32
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Hom(luxA) fwd

<400> 32
cagtgaattc ggtctctacc cgaggtaatg aaacgtttgg                           40

<210> 33
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Hom(luxA) rev

<400> 33
agcttctaga ggtctcttga gcccatattc ttgagccac                            39

<210> 34
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: KanR - ColE1 fwd NcoI

<400> 34
tcagccatgg gttcaacaaa cgggccat                                        28

<210> 35
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: KanR - ColE1 rev PstI

<400> 35
tgacctgcag agtacttgag ttttcgttcc actgag                               36

<210> 36
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: T2Acassette fwd BamHI

<400> 36
tacgagatct gtcgacggat ccgatatccc accctgagac ccac                      44

<210> 37
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: T2A cassette rev NcoI

<400> 37
gtacccatgg gatatcttaa attgagtgag acgagcagg                            39

<210> 38
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Terminator fwd SpeI

<400> 38
tagcactagt ctaaaaagga gcgatgag                                        28

<210> 39
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Terminator rev BamHI

<400> 39
gatcggatcc gtaatctaga gcaacgttc                                       29

<210> 40
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: pE194 ori fwd SpeI

<400> 40
gatcactagt gacgatttag agatgtagag g                                    31

<210> 41
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: pE194 ori rev NcoI

<400> 41
gcatccatgg gctggtgcga aaaaagag                                        28

<210> 42
<211> 28
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: oriT fwd NcoI

<400> 42
atgcccatgg gactaaattt ctgtatgg                                        28

<210> 43
<211> 26
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: oriT rev XbaI

<400> 43
gacttctaga ccccttgtta acgctc                                          26

<210> 44
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Pxyl_cop fwd NsiI

<400> 44
gatcatgcat catatgtcga gttatagaaa atc                                  33

<210> 45
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Pxyl_cop rev SpeI

<400> 45
tgacactagt ctttttttat tccttcatca g                                    31

<210> 46
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Quickchange lux001

<400> 46
gaagatagta ataaagctaa acaagagaca cgtgcattta ttagtg                    46

<210> 47
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Quickchange lux002

<400> 47
gatctcatga cggaatgagg ccgttgcaac gattag                               36

<210> 48
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Quickchange lux003

<400> 48
gatgattatt gagtcaaatg cagatgtgga atttaatcaa ccac                      44

<210> 49
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Artificial oligonucleotide

<400> 49
gttatgcata tgcaaaaaaa c                                               21

<210> 50
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Artificial oligonucleotide

<400> 50
cgcgtacgat gacctcta                                                   18

<210> 51
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Artificial oligonucleotide

<400> 51
accttcttca actaacgg                                                   18

<210> 52
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Artificial oligonucleotide

<400> 52
gaagaaagca gacaagtaag                                                 20

<210> 53
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: sacA 5-prime hom region fwd

<400> 53
cagtgaattc ggtctctacc cgccgggctt atgaagaag                            39

<210> 54
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: sacA 5-prime hom region rev

<400> 54
taggcttcta gaggtctctt cgcatcgggc caagaaatct c                         41

<210> 55
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: sacA 3-prime hom region fwd

<400> 55
agctgaattc ggtctctgag actggattca ctgcatgac                            39

<210> 56
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: sacA 3-prime hom region rev

<400> 56
agcttctaga ggtctcttga gcactggctg ttacttcat                            39

<210> 57
<211> 42
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: rev lux

<400> 57
gcattctaga gaagacgcgc tagcactggt aatggtagcg ac                        42

<210> 58
<211> 46
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: fwd lux

<400> 58
gcatgaattc gaagacgcat atgaaatttg gaaacttttt gcttac                    46

<210> 59
<211> 39
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Cop fwd

<400> 59
gcatgaattc gaagacgcat atggttgtag atagaaaag                            39

<210> 60
<211> 40
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Cop rev

<400> 60
gcattctaga gaagacgcgc tattaaaatg caccgtattc                           40

<210> 61
<211> 41
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: fwd lux

<400> 61
gcatgaattc gaagacgcat atgcttttat aagtcgacag g                         41

<210> 62
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Pveg T2A fwd

<400> 62
cgaggaagac cttcgagagt tctgagaatt ggtatgcc                             38

<210> 63
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Pveg T2A rev

<400> 63
attcgaagac ttatatagag tcctcctgtc gaccttac                             38

<210> 64
<211> 44
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Primer G to A 5-prime of repF

<400> 64
ctatgactaa attttgttaa atgtattagc accgttatta tatc                      44

<210> 65
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: Primer G to A within repF

<400> 65
gggatactga tacagaagat aggatgaaaa aagagc                               36

<210> 66
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: amylase gene amyE fwd

<400> 66
gagaggtctc gacccaaatg tgtgcagccg ctgaaga                              37

<210> 67
<211> 36
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: amylase gene amyE rev

<400> 67
gagagagacc gtcgccgact ctacccatgt cactag                               36

<210> 68
<211> 3123
<212> DNA
<213> Artificial Sequence

<220>
<223> replication origin: functional fragment replication origin
       pBS72 modified

<400> 68
ggttgcggtc tcaccacgcg ctagtttgtc tgcaactgaa aagtttatac cttacctgga     60
acaaatggtt gaaacatacg aggctaatat cggcttatta ggaatagtcc ctgtactaat    120
aaaatcaggt ggatcagttg atcagtatat tttggacgaa gctcggaaag aatttggaga    180
tgacttgctt aattccacaa ttaaattaag ggaaagaata aagcgatttg atgttcaagg    240
aatcacggaa gaagatactc atgataaaga agctctaaaa ctattcaata accttacaat    300
ggaattgatc gaaagggtgg aaggttaatg gtacgaaaat taggggatct acctagaaag    360
ccacaaggcg ataggtcaag cttaaagaac ccttacatgg atcttacaga ttctgaaagt    420
aaagaaacaa cagaggttaa acaaacagaa ccaaaaagaa aaaaagcatt gttgaaaaca    480
atgaaagttg atgtttcaat ccataataag attaaatcgc tgcacgaaat tctggcagca    540
tccgaaggga attcatatta cttagaggat actattgaga gagctattga taagatggtt    600
gagacattac ctgagagcca aaaaactttt tatgaatatg aattaaaaaa aagaaccaac    660
aaaggctgag acagactcca aacgagtctg tttttttaaa aaaaatatta ggagcattga    720
atatatatta gagaattaag aaagacatgg gaataaaaat attttaaatc cagtaaaaat    780
atgataagat tatttcagaa tatgaagaac tctgtttgtt tttgatgaaa aaacaaacaa    840
aaaaaatcca cctaacggaa tctcaattta actaacagcg gccaaactga gaagttaaat    900
ttgagaaggg gaaaaggcgg atttatactt gtatttaact atctccattt taacatttta    960
ttaaacccca tacaagtgaa aatcctcttt tacactgttc ctttaggtga tcgcggaggg   1020
acattatgag tgaagtaaac ctaaaaggaa atacagatga attagtgtat tatcgacagc   1080
aaaccactgg aaataaaatc gccaggaaga gaatcaaaaa agggaaagaa gaagtttatt   1140
atgttgctga aacggaagag aagatatgga cagaagagca aataaaaaac ttttctttag   1200
acaaatttgg tacgcatata ccttacatag aaggtcatta tacaatctta aataattact   1260
tctttgattt ttggggctat tttttaggtg ctgaaggaat tgcgctctat gctcacctaa   1320
ctcgttatgc atacggcagc aaagactttt gctttcctag tctacaaaca atcgctaaaa   1380
aaatggacaa gactcctgtt acagttagag gctacttgaa actgcttgaa aggtacggtt   1440
ttatttggaa ggtaaacgtc cgtaataaaa ccaaggataa cacagaggaa tccccgattt   1500
ttaagattag acgtaaggtt cctttgcttt cagaagaact tttaaatgga aaccctaata   1560
ttgaaattcc agatgacgag gaagcgcatg taaagaaggc tttaaaaaag gaaaaagagg   1620
gacttccaaa ggttttgaaa aaagagcacg atgaatttgt taaaaaaatg atggatgagt   1680
cagaaacaat taatattcca gaggctttac aatatgacac aatgtatgaa gatatactca   1740
gtaaaggaga aattcgaaaa gaaatcaaaa aacaaatacc taatcctaca acatcttttg   1800
agagtatatc aatgacaact gaagaggaaa aagtggacag tactttaaaa agcgaaatgc   1860
aaaatcgtgt ctctaagcct tcttttgata cctggtttaa aaacactaag atcaaaattg   1920
aaaataaaaa ttgtttatta cttgtaccga gtgaatttgc atttgaatgg attaagaaaa   1980
gatatttaga aacaattaaa acagtccttg aagaagctgg atatgttttc gaaaaaatcg   2040
aactaagaaa agtgcaataa actgctgaag tatttcagca gtttttttta tttagaaata   2100
gtgaaaaaaa tataatcagg gaggtatcaa tatttaatga gtactgattt aaatttattt   2160
agactggaat taataattaa cacgtagact aattaaaatt taatgaggga taaagaggat   2220
acaaaaatat taatttcaat ccctattaaa ttttaacaag ggggggatta aaatttaatt   2280
agaggtttat ccacaagaaa agaccctaat aaaattttta ctagggttat aacactgatt   2340
aatttcttaa tgggggaggg attaaaattt aatgacaaag aaaacaatct tttaagaaaa   2400
gcttttaaaa gataataata aaaagagctt tgcgattaag caaaactctt tactttttca   2460
ttgacattat caaattcatc gatttcaaat tgttgttgta tcataaagtt aattctgttt   2520
tgcacaacct tttcaggaat ataaaacaca tctgaggctt gttttataaa ctcagggtcg   2580
ctaaagtcaa tgtaacgtag catatgatat ggtatagctt ccacccaagt tagcctttct   2640
gcttcttctg aatgtttttc atatacttcc atgggtatct ctaaatgatt ttcctcatgt   2700
agcaaggtat gagcaaaaag tttatggaat tgatagttcc tctctttttc ttcaactttt   2760
ttatctaaaa caaacacttt aacatctgag tcaatgtaag cataagatgt ttttccagtc   2820
ataatttcaa tcccaaatct tttagacaga aattctggac gtaaatcttt tggtgaaaga   2880
atttttttat gtagcaatat atccgataca gcaccttcta aaagcgttgg tgaatagggc   2940
attttaccta tctcctctca ttttgtggaa taaaaatagt catattcgtc catctaccta   3000
tcctattatc gaacagttga actttttaat caaggatcag tccttttttt cattattctt   3060
aaactgtgct cttaacttta acaactcgat ttgtttttcc agatcccgct cagagacccg   3120
ttg                                                                 3123

<210> 69
<211> 1221
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: Type-II mRFP cassette

<400> 69
ggttgcggtc tcaaccttta tggttttggt cggcactgcc gacagcctcg cagagcacac     60
actttatgaa tataaagtat agtgtgttat actttacttg gaagtggttg ccggaaagag    120
cgaaaatgcc tcacatttgt gccacctaaa aaggagcgat ttacatatgt cgagggtctt    180
cggtaccttg tcggataaag ctgtgttata ttatgtcttg gtgttaaata cacacgctta    240
acgatttatg cagagggtgc tgcaggcggc agttctgtac aaaaatgacc taagcggagg    300
aaaaaaacca ttatattagg aggaaataac atggcctctt cagaggatgt tattaaagaa    360
tttatgcggt ttaaggtgag gatggaaggc tcggtgaacg gacatgagtt cgaaattgag    420
ggagaaggtg aaggccgccc ttatgaaggt actcagacag cgaaattgaa agtcacgaaa    480
ggcggaccgc tgccgtttgc ttgggacatt ctctcacctc aatttcaata tggctcaaaa    540
gcctacgtaa aacacccggc tgacatccct gattacttaa agctatcctt cccggagggc    600
tttaaatggg aacgagttat gaattttgag gacggcggcg tcgttactgt cacacaggat    660
tcttcccttc aggatggcga atttatttac aaagtaaaac ttcgtggaac taacttccca    720
agcgatggtc ccgtgatgca aaaaaaaaca atgggatggg aagcatctac ggaacgtatg    780
tatccggagg atggagcctt aaagggtgaa atcaaaatgc gcctgaaact taaagatggc    840
ggacactatg acgcggaagt taaaacaaca tatatggcta aaaaaccagt ccaactgccg    900
ggagcatata agacggatat aaagttggac attaccagcc ataatgaaga ttacacgatt    960
gtggaacagt atgagagagc agagggcaga catagcacag gcgcgtaaga attaatgaaa   1020
aataagcggc agcctgcttt tccatgcggg ctgccgctta tcgggttatt gcggccgcgt   1080
ttataatgaa gaccctagcc atattctaac aaatagcata tagaaaaagc tagtgttttt   1140
agcactagct ttttcttcat tctgatgaag gaataaaaaa agcacctgaa aaggtgtctt   1200
tttttccaca gagacccgtt g                                             1221

<210> 70
<211> 1817
<212> DNA
<213> Artificial Sequence

<220>
<223> functional fragment: modified kanamycin resistance gene an
      d ColE1 replication origin

<400> 70
ggttgcggtc tcagctctag ttcaacaaac gggccatatt gttgtataag tgatgaaata     60
ctgaatttaa aacttagttt atatgtggta aaatgtttta atcaagttta ggaggaatta    120
attatgaagt gtaataatgg accaataata atgactagag aagaaagaat gaagattgtt    180
catgaaatta aggaacgaat attggataaa tatggggatg atgttaaggc tattggtgtt    240
tatggctctc ttggtcgtca gactgatggg ccctattcgg atattgagat gatgtgtgtc    300
atgtcaacag aggaagcaga gttcagccat gaatggacaa ccggtgagtg gaaggtggaa    360
gtgaattttg atagcgaaga gattctacta gattatgcat ctcaggtgga atcagattgg    420
ccgcttacac atggtcaatt tttctctatt ttgccgattt atgattcagg tggatactta    480
gagaaagtgt atcaaactgc taaatcggta gaagcccaaa cgttccacga tgcgatttgt    540
gcccttatcg tagaagagct gtttgaatat gcaggcaaat ggcgtaatat tcgtgtgcaa    600
ggaccgacaa catttctacc atccttgact gtacaggtag caatggcagg tgccatgttg    660
attggtctgc atcatcgcat ctgttatacg acgagcgctt cggtcttaac tgaagcagtt    720
aagcaatcag atcttccttc aggttatgac catctgtgcc agttcgtaat gtctggtcaa    780
ctttccgact ctgagaaact tctggaatcg ctagagaatt tctggaatgg gattcaggag    840
tggacagaac gacacggata tatagtggat gtgtcaaaac gcataccatt ttgaatcgtt    900
aagggatcaa ctttgggaga gagttcaaaa ttgatccttt ttttataaca ggaattctga    960
gcaaaaggcc agcaaaaggc caggaaccgt aaaaagcgcg cgttgctggc gtttttccat   1020
aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac   1080
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct   1140
gttccgaccc tcgccgttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg   1200
ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg   1260
ggctgtgtgc acgaaccccc cgttcagccc gacccgtgcg ccttatccgg taactatcgt   1320
cttgagtcca acccggtaag acacgactta tcgccactgg cacgagccac tggtaacagg   1380
attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac   1440
ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga   1500
aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt   1560
gtttgcaacg agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt   1620
tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga   1680
ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc   1740
taaagtatat atgagtacaa ttcaaatctt tttgttccat taaagggcgc gattgctgaa   1800
tacctagaga cccgttg                                                  1817

<210> 71
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification terminator pMutin

<400> 71
cacatttgtg ccacctaaaa aggagcgatg agtcgctttt gtaaatttgg a              51

<210> 72
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification terminator pMutin

<400> 72
caaggtaccg aagaccctcg acatatgtaa tctagagcaa cgttcttgcc a              51

<210> 73
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification pBW424

<400> 73
ttacatatgt cgagggtctt cg                                              22

<210> 74
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: PCR-amplification pBW424

<400> 74
atcgctcctt tttaggtggc                                                 20

<210> 75
<211> 38
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: fwd amyB

<400> 75
tcgcgaagac aatcgcgatc cgcgacatta acgtttac                             38

<210> 76
<211> 37
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: rev amyB

<400> 76
acccgaagac ttacccgatt gataatgaag cggcacg                              37

<210> 77
<211> 33
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: fwd primer, T2A cassette from plasmid p#0
      692 with ter

<400> 77
gcatggtctc atcgaccgga aagagcgaaa atg                                  33

<210> 78
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: rev primer; T2A cassette from plasmid p#0
      692 with ter

<400> 78
cgatggtctc tgctacgcgt ggaaaaaaag acac                                 34

<210> 79
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Artificial oligonucleotide

<400> 79
gagattccgg tcgacaattt gaga                                            24

<210> 80
<211> 24
<212> DNA
<213> Artificial Sequence

<220>
<223> Artificial oligonucleotide

<400> 80
tgagtctcaa attgtcgacc ggaa                                            24

<210> 81
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR luxB fwd

<400> 81
atgtaacagc aaccagtcat c                                               21

<210> 82
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR luxB rev

<400> 82
atccgcaacg gctttatatc                                                 20

<210> 83
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR ter Bli fwd

<400> 83
gtctaagaca tggttccatt cg                                              22

<210> 84
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Oligonucleotide: qPCR ter Bli rev

<400> 84
caaactttgc acattgcatc c                                               21
