                         SEQUENCE LISTING

<110>  Eli Lilly and Company
 
<120>  Process For Preparing A GLP-1/Glucagon Dual Agonist

<130>  X22473

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Lys at position 20 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       ([2-(2-aminoethoxy)-ethoxy]-acetyl)2-(y-Glu)-CO-(CH2)18CO2H

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  Gly at position 34 is amidated

<400>  1

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  2
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is optionally protected by the protecting 
       group PG1, wherein PG1 is a base stable side-chain protecting 
       group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Lysine is protected by the protecting group PG2, 
       wherein PG2 is an ivDde, Dde or Alloc side-chain protecting group

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by the protecting group PG1,
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  2

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  3
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Boc

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Lysine is protected by ivDde

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  3

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  4
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Dnp

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Lysine is protected by ivDde

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  4

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  5
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is optionally protected by the protecting 
       group PG1, wherein PG1 is a base stable side-chain protecting 
       group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by the protecting group PG1,
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  5

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  6
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Boc

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  The sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  The sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  The sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  The sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  The sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  The sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  The sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  The sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  The sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  The sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  The sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  The sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  The sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  The sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  The sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  The sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  The sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  The sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  6

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  7
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is optionally protected by the protecting 
       group PG1, wherein PG1 is a base stable side-chain protecting 
       group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Lysine at position 20 is chemically modified through conjugation 
       to the epsilon-amino group of the K side-chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu(t-butyl))-CO-(
       CH2)18-CO2-(t-butyl)

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by the protecting group PG1,
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  7

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  8
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Boc

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Lysine at position 20 is chemically modified through conjugation 
       to the epsilon-amino group of the K side-chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu(t-butyl))-CO-(
       CH2)18-CO2-(t-butyl)

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  8

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  9
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Phenylalanine is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Lysine is protected by the protecting group PG2, 
       wherein PG2 is an ivDde, Dde or Alloc side-chain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (19)..(19)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Tryptophan is protected by the protecting group PG1,
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (23)..(23)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (27)..(27)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  The C-terminal Glycine is amidated and is bound to resin through 
       this amine group

<400>  9

Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu Lys Lys Ala Lys Glu 
1               5                   10                  15      


Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser Ser Gly 
            20                  25                  


<210>  10
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Phenylalanine is protected by Fmoc

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Lysine is protected by ivDde

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (19)..(19)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (23)..(23)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (27)..(27)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  10

Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu Lys Lys Ala Lys Glu 
1               5                   10                  15      


Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser Ser Gly 
            20                  25                  


<210>  11
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Lysine is protected by the protecting group PG2, 
       wherein PG2 is an ivDde, Dde or Alloc side-chain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Tryptophan is protected by the protecting group PG1,
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  11

Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu Lys Lys Ala Lys 
1               5                   10                  15      


Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser Ser Gly 
            20                  25                  30  


<210>  12
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Threonine is protected by Fmoc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (4)..(4)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (6)..(6)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (14)..(14)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Lysine is protected by ivDde

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (29)..(29)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (30)..(30)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  12

Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu Lys Lys Ala Lys 
1               5                   10                  15      


Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser Ser Gly 
            20                  25                  30  


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<400>  13

His Xaa Gln Gly Thr 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Dnp

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is protected by t-Butyl

<400>  14

His Xaa Gln Gly Thr 
1               5   


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<400>  15

His Xaa Gln Gly 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Dnp

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<400>  16

His Xaa Gln Gly 
1               


<210>  17
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Sidechain of Lysine is protected by the protecting group PG2, 
       wherein PG2 is an ivDde, Dde or Alloc side-chain protecting group

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by the protecting group PG1,
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by the protecting group 
       PG1, wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by the protecting group PG1, 
       wherein PG1 is a base stable side-chain protecting group

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  17

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  18
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Dnp

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  18

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


<210>  19
<211>  34
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  N-terminal Histidine is protected by Boc

<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  Sidechain of Histidine is protected by Dnp

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MOD_RES
<222>  (3)..(3)
<223>  Sidechain of Glutamine is protected by Trityl

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  Sidechain of Threonine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (10)..(10)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (11)..(11)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (12)..(12)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (13)..(13)
<223>  Sidechain of Tyrosine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (15)..(15)
<223>  Sidechain of Aspartic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (16)..(16)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (18)..(18)
<223>  Sidechain of Lysine is protected by Boc

<220>
<221>  MOD_RES
<222>  (20)..(20)
<223>  Lysine at position 20 is chemically modified through conjugation 
       to the epsilon-amino group of the K side-chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu(t-butyl))-CO-(
       CH2)18-CO2-(t-butyl)

<220>
<221>  MOD_RES
<222>  (21)..(21)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (24)..(24)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (25)..(25)
<223>  Sidechain of Tryptophan is protected by Boc

<220>
<221>  MOD_RES
<222>  (28)..(28)
<223>  Sidechain of Glutamic acid is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (32)..(32)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (33)..(33)
<223>  Sidechain of Serine is protected by t-Butyl

<220>
<221>  MOD_RES
<222>  (34)..(34)
<223>  The C-terminal Glycine is amidated and is bound to Sieber resin 
       through this amine group

<400>  19

His Xaa Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Glu 
1               5                   10                  15      


Lys Lys Ala Lys Glu Phe Val Glu Trp Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly 
        


