                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  COMPOSITIONS AND METHODS FOR DETECTING MOLECULAR TARGETS ON 
       CHROMOSOMAL DNA

<130>  NEB-428

<150>  63/038,469
<151>  2020-06-12

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  1

Gly Ser Ala Thr Ile Val 
1               5       


<210>  2
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  2

Gly Ser Ala Thr Gly Thr Ser Ala Gly Ser Thr Gly 
1               5                   10          


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  3

Gly Ser Gly Ser Gly Ser 
1               5       


<210>  4
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  4

Lys Lys Pro Leu Arg Pro Cys Cys Glu Phe His Cys Tyr Asn Leu Thr 
1               5                   10                  15      


Arg Glu Arg Tyr Cys Glu Glu His Arg Tyr Lys Glu Lys Glu Thr Gln 
            20                  25                  30          


Gln Asp Lys Asn Arg Tyr Tyr Asp Arg Phe Lys Arg Asp Lys Glu Ser 
        35                  40                  45              


Thr Ala Phe Tyr Arg Ser Lys Ala Trp Glu Arg Leu Arg Glu Gln Ala 
    50                  55                  60                  


Leu Met Arg Asp Lys Gly Leu Cys Leu His Cys Lys Asn Asn Arg Lys 
65                  70                  75                  80  


Ile Lys Val Ala Asp Met Val Asp His Ile Ile Pro Ile Lys Val Asp 
                85                  90                  95      


Pro Ser Leu Lys Leu Lys Leu Glu Asn Leu Gln Ser Leu Cys Asn Pro 
            100                 105                 110         


Cys His Asn Arg Lys Thr Ala Glu Asp Lys Lys Lys Tyr Gly 
        115                 120                 125     


<210>  5
<211>  317
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  5

Met Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu 
1               5                   10                  15      


Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp 
            20                  25                  30          


Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Pro Glu 
        35                  40                  45              


Val Ile Asp Ala Ser Glu Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu 
    50                  55                  60                  


Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu Ala Val 
65                  70                  75                  80  


Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn 
                85                  90                  95      


Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr 
            100                 105                 110         


Val Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu Leu Thr Pro Ala 
        115                 120                 125             


Val Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu 
    130                 135                 140                 


Thr Thr Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys 
145                 150                 155                 160 


Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp 
                165                 170                 175     


Ala Thr Lys Thr Phe Thr Val Thr Glu Gly Ser Ala Thr Ile Val Lys 
            180                 185                 190         


Lys Pro Leu Arg Pro Cys Cys Glu Phe His Cys Tyr Asn Leu Thr Arg 
        195                 200                 205             


Glu Arg Tyr Cys Glu Glu His Arg Tyr Lys Glu Lys Glu Thr Gln Gln 
    210                 215                 220                 


Asp Lys Asn Arg Tyr Tyr Asp Arg Phe Lys Arg Asp Lys Glu Ser Thr 
225                 230                 235                 240 


Ala Phe Tyr Arg Ser Lys Ala Trp Glu Arg Leu Arg Glu Gln Ala Leu 
                245                 250                 255     


Met Arg Asp Lys Gly Leu Cys Leu His Cys Lys Asn Asn Arg Lys Ile 
            260                 265                 270         


Lys Val Ala Asp Met Val Asp His Ile Ile Pro Ile Lys Val Asp Pro 
        275                 280                 285             


Ser Leu Lys Leu Lys Leu Glu Asn Leu Gln Ser Leu Cys Asn Pro Cys 
    290                 295                 300                 


His Asn Arg Lys Thr Ala Glu Asp Lys Lys Lys Tyr Gly 
305                 310                 315         


<210>  6
<211>  373
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  6

Ala Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu 
1               5                   10                  15      


Asn Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser 
            20                  25                  30          


Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln 
        35                  40                  45              


Lys Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Lys 
    50                  55                  60                  


Phe Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro 
65                  70                  75                  80  


Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
                85                  90                  95      


Asp Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn 
            100                 105                 110         


Glu Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln 
        115                 120                 125             


Asn Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln 
    130                 135                 140                 


Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala 
145                 150                 155                 160 


Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
                165                 170                 175     


Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
            180                 185                 190         


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln 
        195                 200                 205             


Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala 
    210                 215                 220                 


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Glu Gly Ser Ala Thr Gly 
225                 230                 235                 240 


Thr Ser Ala Gly Ser Thr Gly Lys Lys Pro Leu Arg Pro Cys Cys Glu 
                245                 250                 255     


Phe His Cys Tyr Asn Leu Thr Arg Glu Arg Tyr Cys Glu Glu His Arg 
            260                 265                 270         


Tyr Lys Glu Lys Glu Thr Gln Gln Asp Lys Asn Arg Tyr Tyr Asp Arg 
        275                 280                 285             


Phe Lys Arg Asp Lys Glu Ser Thr Ala Phe Tyr Arg Ser Lys Ala Trp 
    290                 295                 300                 


Glu Arg Leu Arg Glu Gln Ala Leu Met Arg Asp Lys Gly Leu Cys Leu 
305                 310                 315                 320 


His Cys Lys Asn Asn Arg Lys Ile Lys Val Ala Asp Met Val Asp His 
                325                 330                 335     


Ile Ile Pro Ile Lys Val Asp Pro Ser Leu Lys Leu Lys Leu Glu Asn 
            340                 345                 350         


Leu Gln Ser Leu Cys Asn Pro Cys His Asn Arg Lys Thr Ala Glu Asp 
        355                 360                 365             


Lys Lys Lys Tyr Gly 
    370             


<210>  7
<211>  539
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  7

Met Lys Gly Glu Thr Thr Thr Glu Ala Val Asp Ala Ala Thr Ala Glu 
1               5                   10                  15      


Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Glu Trp 
            20                  25                  30          


Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Lys Pro Glu 
        35                  40                  45              


Val Ile Asp Ala Ser Glu Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu 
    50                  55                  60                  


Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Glu Ala Val 
65                  70                  75                  80  


Asp Ala Ala Thr Ala Glu Lys Val Phe Lys Gln Tyr Ala Asn Asp Asn 
                85                  90                  95      


Gly Val Asp Gly Glu Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr 
            100                 105                 110         


Val Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu Leu Thr Pro Ala 
        115                 120                 125             


Val Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu 
    130                 135                 140                 


Thr Thr Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys 
145                 150                 155                 160 


Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp 
                165                 170                 175     


Ala Thr Lys Thr Phe Thr Val Thr Glu Gly Ser Ala Thr Ile Val Met 
            180                 185                 190         


Tyr Ile Tyr Met Ser Thr Pro Gln Ala Lys Thr Lys Tyr Tyr Glu Gln 
        195                 200                 205             


Arg Phe Val Asn Asp Phe Tyr Lys Glu Leu Glu Arg Asn Lys Val Ser 
    210                 215                 220                 


Leu Pro Val Thr Ile Val Leu Lys Asp Asn Leu Gly Ile Lys Gln Val 
225                 230                 235                 240 


Ile Gln Asn Gly Ser Gly Val Arg Val Leu Arg Asp Lys Ala Asn Ala 
                245                 250                 255     


Lys Ser Pro Ser Lys Ile Lys Ser Glu Glu Leu Gly Arg His Val Thr 
            260                 265                 270         


Ser Lys Ala Asp Ile Ala Leu Phe Thr Glu Glu Lys Asn Gly Thr Lys 
        275                 280                 285             


Val Asp Val Ala Trp Ile Ser Pro Gln Ser His Lys Asp Phe Leu Gly 
    290                 295                 300                 


Lys Lys Ile Thr Pro Ala Gln Tyr Phe Asp Ala Ser Ser Asp Val Met 
305                 310                 315                 320 


Phe Lys Thr Lys Ile Gly Gln Pro Lys Glu Ile Lys Glu Leu Lys Asn 
                325                 330                 335     


Lys Met Ile Ser Leu Ser Val Pro Leu Thr Ala Thr Lys Tyr Cys Trp 
            340                 345                 350         


Pro Lys Tyr Lys Ser Gly Thr Ser Leu Arg Ile Trp Asp Asp Val Gln 
        355                 360                 365             


Ser Thr Ile Leu Met Asn Met Ala Ile Phe Gly Val Glu Phe Gly Lys 
    370                 375                 380                 


Ala Tyr Cys Arg Asn Asn Ala Asn Ile Leu Met Val Gly Asp Pro Leu 
385                 390                 395                 400 


Ile Glu Val Lys Asp Asp Lys Thr Ile Ile Leu Thr Thr Lys Glu Asn 
                405                 410                 415     


Gly Phe Ser Leu Ala Asn Gly Phe Ala Glu Tyr Ile Pro Ser Lys Asp 
            420                 425                 430         


Lys Pro Ile Phe Phe Thr Lys Pro Thr Ser Gly Lys Lys Thr Val Val 
        435                 440                 445             


Asp Gly Lys Thr Ile Glu Gly Val Ser Val Trp Ile Ile Tyr Arg Ser 
    450                 455                 460                 


Tyr Ala Gly Ser Lys Asn Arg Lys Ile Asp Asp Val Leu Lys Asn Lys 
465                 470                 475                 480 


Ile Glu Leu Ile Ser Ser Ser Cys Ser Val Lys Lys Lys Asp Asn Phe 
                485                 490                 495     


Val Ser Ile Met Gln Ser Lys Lys Ile Thr Ser Pro Pro Lys Ser Lys 
            500                 505                 510         


Lys Ile Thr Ser Pro Pro Lys Ser Lys Lys Ile Thr Ser Pro Ser Lys 
        515                 520                 525             


Ser Lys Lys Ile Thr Asn Phe Phe Met Lys Lys 
    530                 535                 


<210>  8
<211>  595
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  8

Ala Ala Gln His Asp Glu Ala Gln Gln Asn Ala Phe Tyr Gln Val Leu 
1               5                   10                  15      


Asn Met Pro Asn Leu Asn Ala Asp Gln Arg Asn Gly Phe Ile Gln Ser 
            20                  25                  30          


Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Val Leu Gly Glu Ala Gln 
        35                  40                  45              


Lys Leu Asn Asp Ser Gln Ala Pro Lys Ala Asp Ala Gln Gln Asn Lys 
    50                  55                  60                  


Phe Asn Lys Asp Gln Gln Ser Ala Phe Tyr Glu Ile Leu Asn Met Pro 
65                  70                  75                  80  


Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp 
                85                  90                  95      


Asp Pro Ser Gln Ser Thr Asn Val Leu Gly Glu Ala Lys Lys Leu Asn 
            100                 105                 110         


Glu Ser Gln Ala Pro Lys Ala Asp Asn Asn Phe Asn Lys Glu Gln Gln 
        115                 120                 125             


Asn Ala Phe Tyr Glu Ile Leu Asn Met Pro Asn Leu Asn Glu Glu Gln 
    130                 135                 140                 


Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala 
145                 150                 155                 160 


Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys 
                165                 170                 175     


Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
            180                 185                 190         


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln 
        195                 200                 205             


Ser Leu Lys Asp Asp Pro Ser Val Ser Lys Glu Ile Leu Ala Glu Ala 
    210                 215                 220                 


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Glu Gly Ser Ala Thr Gly 
225                 230                 235                 240 


Thr Ser Ala Gly Ser Thr Gly Met Tyr Ile Tyr Met Ser Thr Pro Gln 
                245                 250                 255     


Ala Lys Thr Lys Tyr Tyr Glu Gln Arg Phe Val Asn Asp Phe Tyr Lys 
            260                 265                 270         


Glu Leu Glu Arg Asn Lys Val Ser Leu Pro Val Thr Ile Val Leu Lys 
        275                 280                 285             


Asp Asn Leu Gly Ile Lys Gln Val Ile Gln Asn Gly Ser Gly Val Arg 
    290                 295                 300                 


Val Leu Arg Asp Lys Ala Asn Ala Lys Ser Pro Ser Lys Ile Lys Ser 
305                 310                 315                 320 


Glu Glu Leu Gly Arg His Val Thr Ser Lys Ala Asp Ile Ala Leu Phe 
                325                 330                 335     


Thr Glu Glu Lys Asn Gly Thr Lys Val Asp Val Ala Trp Ile Ser Pro 
            340                 345                 350         


Gln Ser His Lys Asp Phe Leu Gly Lys Lys Ile Thr Pro Ala Gln Tyr 
        355                 360                 365             


Phe Asp Ala Ser Ser Asp Val Met Phe Lys Thr Lys Ile Gly Gln Pro 
    370                 375                 380                 


Lys Glu Ile Lys Glu Leu Lys Asn Lys Met Ile Ser Leu Ser Val Pro 
385                 390                 395                 400 


Leu Thr Ala Thr Lys Tyr Cys Trp Pro Lys Tyr Lys Ser Gly Thr Ser 
                405                 410                 415     


Leu Arg Ile Trp Asp Asp Val Gln Ser Thr Ile Leu Met Asn Met Ala 
            420                 425                 430         


Ile Phe Gly Val Glu Phe Gly Lys Ala Tyr Cys Arg Asn Asn Ala Asn 
        435                 440                 445             


Ile Leu Met Val Gly Asp Pro Leu Ile Glu Val Lys Asp Asp Lys Thr 
    450                 455                 460                 


Ile Ile Leu Thr Thr Lys Glu Asn Gly Phe Ser Leu Ala Asn Gly Phe 
465                 470                 475                 480 


Ala Glu Tyr Ile Pro Ser Lys Asp Lys Pro Ile Phe Phe Thr Lys Pro 
                485                 490                 495     


Thr Ser Gly Lys Lys Thr Val Val Asp Gly Lys Thr Ile Glu Gly Val 
            500                 505                 510         


Ser Val Trp Ile Ile Tyr Arg Ser Tyr Ala Gly Ser Lys Asn Arg Lys 
        515                 520                 525             


Ile Asp Asp Val Leu Lys Asn Lys Ile Glu Leu Ile Ser Ser Ser Cys 
    530                 535                 540                 


Ser Val Lys Lys Lys Asp Asn Phe Val Ser Ile Met Gln Ser Lys Lys 
545                 550                 555                 560 


Ile Thr Ser Pro Pro Lys Ser Lys Lys Ile Thr Ser Pro Pro Lys Ser 
                565                 570                 575     


Lys Lys Ile Thr Ser Pro Ser Lys Ser Lys Lys Ile Thr Asn Phe Phe 
            580                 585                 590         


Met Lys Lys 
        595 


<210>  9
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (31)..(31)
<223>  n is fluorescein

<400>  9
ccagatcgtt caccagtaca cagtaattcg n                                      31


