                               SEQUENCE LISTING

<110> GLAXOSMITHKLINE BIOLOGICALS SA
 
<120> BACTERIAL IMMUNIZATION USING NANOPARTICLE VACCINE

<130> VU66934 WO

<140>
<141>

<150> 63/038,277
<151> 2020-06-12

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 133
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 1
Met Ala Lys Leu Glu Thr Val Thr Leu Gly Asn Ile Gly Lys Asp Gly 
1               5                   10                  15      


Lys Gln Thr Leu Val Leu Asn Pro Arg Gly Val Asn Pro Thr Asn Gly 
            20                  25                  30          


Val Ala Ser Leu Ser Gln Ala Gly Ala Val Pro Ala Leu Glu Lys Arg 
        35                  40                  45              


Val Thr Val Ser Val Ser Gln Pro Ser Arg Asn Arg Lys Asn Tyr Lys 
    50                  55                  60                  


Val Gln Val Lys Ile Gln Asn Pro Thr Ala Cys Thr Ala Asn Gly Ser 
65                  70                  75                  80  


Cys Asp Pro Ser Val Thr Arg Gln Ala Tyr Ala Asp Val Thr Phe Ser 
                85                  90                  95      


Phe Thr Gln Tyr Ser Thr Asp Glu Glu Arg Ala Phe Val Arg Thr Glu 
            100                 105                 110         


Leu Ala Ala Leu Leu Ala Ser Pro Leu Leu Ile Asp Ala Ile Asp Gln 
        115                 120                 125             


Leu Asn Pro Ala Tyr 
    130             


<210> 2
<211> 205
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 2
Met Lys Met Glu Glu Leu Phe Lys Lys His Lys Ile Val Ala Val Leu 
1               5                   10                  15      


Arg Ala Asn Ser Val Glu Glu Ala Lys Lys Lys Ala Leu Ala Val Phe 
            20                  25                  30          


Leu Gly Gly Val His Leu Ile Glu Ile Thr Phe Thr Val Pro Asp Ala 
        35                  40                  45              


Asp Thr Val Ile Lys Glu Leu Ser Phe Leu Lys Glu Met Gly Ala Ile 
    50                  55                  60                  


Ile Gly Ala Gly Thr Val Thr Ser Val Glu Gln Ala Arg Lys Ala Val 
65                  70                  75                  80  


Glu Ser Gly Ala Glu Phe Ile Val Ser Pro His Leu Asp Glu Glu Ile 
                85                  90                  95      


Ser Gln Phe Ala Lys Glu Lys Gly Val Phe Tyr Met Pro Gly Val Met 
            100                 105                 110         


Thr Pro Thr Glu Leu Val Lys Ala Met Lys Leu Gly His Thr Ile Leu 
        115                 120                 125             


Lys Leu Phe Pro Gly Glu Val Val Gly Pro Gln Phe Val Lys Ala Met 
    130                 135                 140                 


Lys Gly Pro Phe Pro Asn Val Lys Phe Val Pro Thr Gly Gly Val Asn 
145                 150                 155                 160 


Leu Asp Asn Val Cys Glu Trp Phe Lys Ala Gly Val Leu Ala Val Gly 
                165                 170                 175     


Val Gly Ser Ala Leu Val Lys Gly Thr Pro Val Glu Val Ala Glu Lys 
            180                 185                 190         


Ala Lys Ala Phe Val Glu Lys Ile Arg Gly Cys Thr Glu 
        195                 200                 205 


<210> 3
<211> 155
<212> PRT
<213> Streptococcus agalactiae

<400> 3
Met Lys Phe Glu Lys Thr Lys Glu Ile Leu Asn Gln Leu Val Ala Asp 
1               5                   10                  15      


Leu Ser Gln Phe Ser Val Val Ile His Gln Thr His Trp Tyr Met Arg 
            20                  25                  30          


Gly Pro Glu Phe Leu Thr Leu His Pro Gln Met Asp Glu Tyr Met Asp 
        35                  40                  45              


Gln Ile Asn Glu Gln Leu Asp Val Val Ser Glu Arg Leu Ile Thr Leu 
    50                  55                  60                  


Asp Gly Ser Pro Phe Ser Thr Leu Arg Glu Phe Ala Glu Asn Thr Lys 
65                  70                  75                  80  


Ile Glu Asp Glu Ile Gly Asn Trp Asp Arg Thr Ile Pro Glu Arg Met 
                85                  90                  95      


Glu Lys Leu Val Ala Gly Tyr Arg Tyr Leu Ala Asp Leu Tyr Ala Lys 
            100                 105                 110         


Gly Ile Glu Val Ser Gly Glu Glu Gly Asp Asp Cys Thr Gln Asp Ile 
        115                 120                 125             


Phe Ile Ala Asn Lys Thr Asp Ile Glu Lys Asn Ile Trp Met Leu Gln 
    130                 135                 140                 


Ala Lys Leu Gly Lys Ala Pro Gly Ile Asp Ala 
145                 150                 155 


<210> 4
<211> 153
<212> PRT
<213> Streptococcus agalactiae

<400> 4
Met Val Tyr Glu Lys Thr Gln Ala Val Leu Asn Gln Ala Val Ala Asp 
1               5                   10                  15      


Leu Ser Lys Ala Ser Ser Ile Val His Gln Thr His Trp Tyr Met Arg 
            20                  25                  30          


Gly Pro Gly Phe Phe Tyr Leu His Pro Lys Met Asp Glu Leu Met Asp 
        35                  40                  45              


Glu Leu Asn Glu Thr Leu Asp Val Val Ser Glu Arg Leu Ile Thr Ile 
    50                  55                  60                  


Gly Gly Ala Pro Tyr Ser Thr Leu Lys Glu Phe Asp Glu His Ser Lys 
65                  70                  75                  80  


Leu Thr Glu Glu Val Gly Ser Tyr Asp Lys Ser Met Thr Glu Arg Leu 
                85                  90                  95      


Glu Lys Leu Ala Asp Val Tyr Ala Tyr Leu Ser Asp Leu Phe Gln Glu 
            100                 105                 110         


Gly Leu Asp Val Ser Asp Glu Glu Gly Asp Asp Pro Ser Asn Gly Ile 
        115                 120                 125             


Phe Ser Asp Ala Gln Asp Ala Val Gln Lys Thr Leu Trp Met Leu Gln 
    130                 135                 140                 


Ala Glu Ile Gly Arg Ser Ser Gly Leu 
145                 150             


<210> 5
<211> 165
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 5
Met Lys Phe Glu Lys Thr Lys Glu Ile Leu Asn Gln Leu Val Ala Asp 
1               5                   10                  15      


Leu Ser Gln Phe Ser Val Val Ile His Gln Thr His Trp Tyr Met Arg 
            20                  25                  30          


Gly Pro Glu Phe Leu Thr Leu His Pro Gln Met Asp Glu Tyr Met Asp 
        35                  40                  45              


Gln Ile Asn Glu Gln Leu Asp Val Val Ser Glu Arg Leu Ile Thr Leu 
    50                  55                  60                  


Asp Gly Ser Pro Phe Ser Thr Leu Arg Glu Phe Ala Glu Asn Thr Lys 
65                  70                  75                  80  


Ile Glu Asp Glu Ile Gly Asn Trp Asp Arg Thr Ile Pro Glu Arg Met 
                85                  90                  95      


Glu Lys Leu Val Ala Gly Tyr Arg Tyr Leu Ala Asp Leu Tyr Ala Lys 
            100                 105                 110         


Gly Ile Glu Val Ser Gly Glu Glu Gly Asp Asp Ser Thr Gln Asp Ile 
        115                 120                 125             


Phe Ile Ala Asn Lys Thr Asp Ile Glu Lys Asn Ile Trp Met Leu Gln 
    130                 135                 140                 


Ala Lys Leu Gly Lys Ala Pro Gly Ile Asp Ala Gly Ser Ser Gly His 
145                 150                 155                 160 


His His His His His 
                165 


<210> 6
<211> 163
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 6
Met Val Tyr Glu Lys Thr Gln Ala Val Leu Asn Gln Ala Val Ala Asp 
1               5                   10                  15      


Leu Ser Lys Ala Ser Ser Ile Val His Gln Thr His Trp Tyr Met Arg 
            20                  25                  30          


Gly Pro Gly Phe Phe Tyr Leu His Pro Lys Met Asp Glu Leu Met Asp 
        35                  40                  45              


Glu Leu Asn Glu Thr Leu Asp Val Val Ser Glu Arg Leu Ile Thr Ile 
    50                  55                  60                  


Gly Gly Ala Pro Tyr Ser Thr Leu Lys Glu Phe Asp Glu His Ser Lys 
65                  70                  75                  80  


Leu Thr Glu Glu Val Gly Ser Tyr Asp Lys Ser Met Thr Glu Arg Leu 
                85                  90                  95      


Glu Lys Leu Ala Asp Val Tyr Ala Tyr Leu Ser Asp Leu Phe Gln Glu 
            100                 105                 110         


Gly Leu Asp Val Ser Asp Glu Glu Gly Asp Asp Pro Ser Asn Gly Ile 
        115                 120                 125             


Phe Ser Asp Ala Gln Asp Ala Val Gln Lys Thr Leu Trp Met Leu Gln 
    130                 135                 140                 


Ala Glu Ile Gly Arg Ser Ser Gly Leu Gly Ser Ser Gly His His His 
145                 150                 155                 160 


His His His 
            


<210> 7
<211> 185
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 7
Met Thr Asn Thr Leu Val Glu Asn Ile Tyr Ala Ser Val Thr His Asn 
1               5                   10                  15      


Ile Ser Lys Lys Glu Ala Ser Lys Asn Glu Lys Thr Gln Ala Val Leu 
            20                  25                  30          


Asn Gln Ala Val Ala Asp Leu Ser Lys Ala Ser Ser Ile Val His Gln 
        35                  40                  45              


Thr His Trp Tyr Met Arg Gly Pro Gly Phe Phe Tyr Leu His Pro Lys 
    50                  55                  60                  


Met Asp Glu Leu Met Asp Glu Leu Asn Glu Thr Leu Asp Val Val Ser 
65                  70                  75                  80  


Glu Arg Leu Ile Thr Ile Gly Gly Ala Pro Tyr Ser Thr Leu Lys Glu 
                85                  90                  95      


Phe Asp Glu His Ser Lys Leu Thr Glu Glu Val Gly Ser Tyr Asp Lys 
            100                 105                 110         


Ser Met Thr Glu Arg Leu Glu Lys Leu Ala Asp Val Tyr Ala Tyr Leu 
        115                 120                 125             


Ser Asp Leu Phe Gln Glu Gly Leu Asp Val Ser Asp Glu Glu Gly Asp 
    130                 135                 140                 


Asp Pro Ser Asn Gly Ile Phe Ser Asp Ala Gln Asp Ala Val Gln Lys 
145                 150                 155                 160 


Thr Leu Trp Met Leu Gln Ala Glu Ile Gly Arg Ser Ser Gly Leu Gly 
                165                 170                 175     


Ser Ser Gly His His His His His His 
            180                 185 


<210> 8
<211> 25
<212> PRT
<213> Streptococcus pyogenes

<400> 8
Met Thr Asn Thr Leu Val Glu Asn Ile Tyr Ala Ser Val Thr His Asn 
1               5                   10                  15      


Ile Ser Lys Lys Glu Ala Ser Lys Asn 
            20                  25  


<210> 9
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 9
Gly Ser Gly Ser Gly Ser Gly Ser Gly Ser 
1               5                   10  


<210> 10
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      peptide"

<400> 10
Gly Ser Ser Gly His 
1               5   


<210> 11
<211> 221
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      polypeptide"

<400> 11
Met Lys Met Glu Glu Leu Phe Lys Lys His Lys Ile Val Ala Val Leu 
1               5                   10                  15      


Arg Ala Asn Ser Val Glu Glu Ala Lys Lys Lys Ala Leu Ala Val Phe 
            20                  25                  30          


Leu Gly Gly Val His Leu Ile Glu Ile Thr Phe Thr Val Pro Asp Ala 
        35                  40                  45              


Asp Thr Val Ile Lys Glu Leu Ser Phe Leu Lys Glu Met Gly Ala Ile 
    50                  55                  60                  


Ile Gly Ala Gly Thr Val Thr Ser Val Glu Gln Ala Arg Lys Ala Val 
65                  70                  75                  80  


Glu Ser Gly Ala Glu Phe Ile Val Ser Pro His Leu Asp Glu Glu Ile 
                85                  90                  95      


Ser Gln Phe Ala Lys Glu Lys Gly Val Phe Tyr Met Pro Gly Val Met 
            100                 105                 110         


Thr Pro Thr Glu Leu Val Lys Ala Met Lys Leu Gly His Thr Ile Leu 
        115                 120                 125             


Lys Leu Phe Pro Gly Glu Val Val Gly Pro Gln Phe Val Lys Ala Met 
    130                 135                 140                 


Lys Gly Pro Phe Pro Asn Val Lys Phe Val Pro Thr Gly Gly Val Asn 
145                 150                 155                 160 


Leu Asp Asn Val Cys Glu Trp Phe Lys Ala Gly Val Leu Ala Val Gly 
                165                 170                 175     


Val Gly Ser Ala Leu Val Lys Gly Thr Pro Val Glu Val Ala Glu Lys 
            180                 185                 190         


Ala Lys Ala Phe Val Glu Lys Ile Arg Gly Cys Thr Glu Gly Ser Gly 
        195                 200                 205             


Ser Gly Ser Gly Ser Gly Ser His His His His His His 
    210                 215                 220     


