                         SEQUENCE LISTING

<110>  Quell Therapeutics Ltd
       King's College London
 
<120>  Chimeric receptor

<130>  20.149488/02

<150>  GB2008855.5
<151>  2020-06-11

<150>  GB2017263.1
<151>  2020-10-30

<160>  70    

<170>  PatentIn version 3.5

<210>  1
<211>  230
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Val Glu His Ser Ile Ser 
145                 150                 155                 160 


Arg Ser Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu 
                165                 170                 175     


Leu Leu Leu Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala 
            180                 185                 190         


Leu Trp Ala Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro 
        195                 200                 205             


Ser Glu Leu Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu 
    210                 215                 220                 


Pro Gly Leu Arg Asp Thr 
225                 230 


<210>  2
<211>  174
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Val Glu His Ser Ile Ser 
145                 150                 155                 160 


Arg Ser Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser 
                165                 170                 


<210>  3
<211>  156
<212>  PRT
<213>  Homo sapiens

<400>  3

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro Gly Glu 
        115                 120                 125             


Ser Glu Ser Phe Glu Asp Ala His Val Glu His Ser Ile Ser Arg Ser 
    130                 135                 140                 


Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser 
145                 150                 155     


<210>  4
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant ligand binding domain of human TREM2


<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  X is a basic amino acid, preferably K or R

<400>  4

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Xaa Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


<210>  5
<211>  120
<212>  PRT
<213>  Homo sapiens

<400>  5

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Arg Asp Ala 
        115                 120 


<210>  6
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant ligand binding domain of TREM 2


<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  X is a basic amino acid, preferably K or R

<400>  6

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Xaa Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Arg Asp Ala 
        115                 120 


<210>  7
<211>  156
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant ligand binding domain of TREM 2


<220>
<221>  VARIANT
<222>  (139)..(140)
<223>  X at position 139 is not H and/or X at position 140 is not S

<400>  7

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro Gly Glu 
        115                 120                 125             


Ser Glu Ser Phe Glu Asp Ala His Val Glu Xaa Xaa Ile Ser Arg Ser 
    130                 135                 140                 


Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser 
145                 150                 155     


<210>  8
<211>  156
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant ligand binding domain of TREM 2


<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  X is a basic amino acid, preferably K or R

<220>
<221>  VARIANT
<222>  (139)..(140)
<223>  X at position 139 is not H and/or X at position 140 is not S

<400>  8

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Xaa Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro Gly Glu 
        115                 120                 125             


Ser Glu Ser Phe Glu Asp Ala His Val Glu Xaa Xaa Ile Ser Arg Ser 
    130                 135                 140                 


Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser 
145                 150                 155     


<210>  9
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  10
<211>  18
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala 
        


<210>  11
<211>  43
<212>  PRT
<213>  Homo sapiens

<400>  11

Ile Glu Val Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn 
1               5                   10                  15      


Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys 
            20                  25                  30          


Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro 
        35                  40              


<210>  12
<211>  27
<212>  PRT
<213>  Homo sapiens

<400>  12

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  13
<211>  112
<212>  PRT
<213>  Homo sapiens

<400>  13

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  14
<211>  286
<212>  PRT
<213>  Homo sapiens

<400>  14

Asn Cys Arg Asn Thr Gly Pro Trp Leu Lys Lys Val Leu Lys Cys Asn 
1               5                   10                  15      


Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln Leu Ser Ser Glu His Gly 
            20                  25                  30          


Gly Asp Val Gln Lys Trp Leu Ser Ser Pro Phe Pro Ser Ser Ser Phe 
        35                  40                  45              


Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser Pro Leu Glu Val Leu Glu 
    50                  55                  60                  


Arg Asp Lys Val Thr Gln Leu Leu Leu Gln Gln Asp Lys Val Pro Glu 
65                  70                  75                  80  


Pro Ala Ser Leu Ser Ser Asn His Ser Leu Thr Ser Cys Phe Thr Asn 
                85                  90                  95      


Gln Gly Tyr Phe Phe Phe His Leu Pro Asp Ala Leu Glu Ile Glu Ala 
            100                 105                 110         


Cys Gln Val Tyr Phe Thr Tyr Asp Pro Tyr Ser Glu Glu Asp Pro Asp 
        115                 120                 125             


Glu Gly Val Ala Gly Ala Pro Thr Gly Ser Ser Pro Gln Pro Leu Gln 
    130                 135                 140                 


Pro Leu Ser Gly Glu Asp Asp Ala Tyr Cys Thr Phe Pro Ser Arg Asp 
145                 150                 155                 160 


Asp Leu Leu Leu Phe Ser Pro Ser Leu Leu Gly Gly Pro Ser Pro Pro 
                165                 170                 175     


Ser Thr Ala Pro Gly Gly Ser Gly Ala Gly Glu Glu Arg Met Pro Pro 
            180                 185                 190         


Ser Leu Gln Glu Arg Val Pro Arg Asp Trp Asp Pro Gln Pro Leu Gly 
        195                 200                 205             


Pro Pro Thr Pro Gly Val Pro Asp Leu Val Asp Phe Gln Pro Pro Pro 
    210                 215                 220                 


Glu Leu Val Leu Arg Glu Ala Gly Glu Glu Val Pro Asp Ala Gly Pro 
225                 230                 235                 240 


Arg Glu Gly Val Ser Phe Pro Trp Ser Arg Pro Pro Gly Gln Gly Glu 
                245                 250                 255     


Phe Arg Ala Leu Asn Ala Arg Leu Pro Leu Asn Thr Asp Ala Tyr Leu 
            260                 265                 270         


Ser Leu Gln Glu Leu Gln Gly Gln Asp Pro Thr His Leu Val 
        275                 280                 285     


<210>  15
<211>  195
<212>  PRT
<213>  Homo sapiens

<400>  15

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Leu Glu Glu Ser Glu Lys Gln Arg Leu Gly Gly Asp Val 
65                  70                  75                  80  


Gln Ser Pro Asn Cys Pro Ser Glu Asp Val Val Ile Thr Pro Glu Ser 
                85                  90                  95      


Phe Gly Arg Asp Ser Ser Leu Thr Cys Leu Ala Gly Asn Val Ser Ala 
            100                 105                 110         


Cys Asp Ala Pro Ile Leu Ser Ser Ser Arg Ser Leu Asp Cys Arg Glu 
        115                 120                 125             


Ser Gly Lys Asn Gly Pro His Val Tyr Gln Asp Leu Leu Leu Ser Leu 
    130                 135                 140                 


Gly Thr Thr Asn Ser Thr Leu Pro Pro Pro Phe Ser Leu Gln Ser Gly 
145                 150                 155                 160 


Ile Leu Thr Leu Asn Pro Val Ala Gln Gly Gln Pro Ile Leu Thr Ser 
                165                 170                 175     


Leu Gly Ser Asn Gln Glu Glu Ala Tyr Val Thr Met Ser Ser Phe Tyr 
            180                 185                 190         


Gln Asn Gln 
        195 


<210>  16
<211>  230
<212>  PRT
<213>  Homo sapiens

<400>  16

Lys Leu Ser Pro Arg Val Lys Arg Ile Phe Tyr Gln Asn Val Pro Ser 
1               5                   10                  15      


Pro Ala Met Phe Phe Gln Pro Leu Tyr Ser Val His Asn Gly Asn Phe 
            20                  25                  30          


Gln Thr Trp Met Gly Ala His Gly Ala Gly Val Leu Leu Ser Gln Asp 
        35                  40                  45              


Cys Ala Gly Thr Pro Gln Gly Ala Leu Glu Pro Cys Val Gln Glu Ala 
    50                  55                  60                  


Thr Ala Leu Leu Thr Cys Gly Pro Ala Arg Pro Trp Lys Ser Val Ala 
65                  70                  75                  80  


Leu Glu Glu Glu Gln Glu Gly Pro Gly Thr Arg Leu Pro Gly Asn Leu 
                85                  90                  95      


Ser Ser Glu Asp Val Leu Pro Ala Gly Cys Thr Glu Trp Arg Val Gln 
            100                 105                 110         


Thr Leu Ala Tyr Leu Pro Gln Glu Asp Trp Ala Pro Thr Ser Leu Thr 
        115                 120                 125             


Arg Pro Ala Pro Pro Asp Ser Glu Gly Ser Arg Ser Ser Ser Ser Ser 
    130                 135                 140                 


Ser Ser Ser Asn Asn Asn Asn Tyr Cys Ala Leu Gly Cys Tyr Gly Gly 
145                 150                 155                 160 


Trp His Leu Ser Ala Leu Pro Gly Asn Thr Gln Ser Ser Gly Pro Ile 
                165                 170                 175     


Pro Ala Leu Ala Cys Gly Leu Ser Cys Asp His Gln Gly Leu Glu Thr 
            180                 185                 190         


Gln Gln Gly Val Ala Trp Val Leu Ala Gly His Cys Gln Arg Pro Gly 
        195                 200                 205             


Leu His Glu Asp Leu Gln Gly Met Leu Leu Pro Ser Val Leu Ser Lys 
    210                 215                 220                 


Ala Arg Ser Trp Thr Phe 
225                 230 


<210>  17
<211>  569
<212>  PRT
<213>  Homo sapiens

<400>  17

Lys Ile Lys Lys Glu Trp Trp Asp Gln Ile Pro Asn Pro Ala Arg Ser 
1               5                   10                  15      


Arg Leu Val Ala Ile Ile Ile Gln Asp Ala Gln Gly Ser Gln Trp Glu 
            20                  25                  30          


Lys Arg Ser Arg Gly Gln Glu Pro Ala Lys Cys Pro His Trp Lys Asn 
        35                  40                  45              


Cys Leu Thr Lys Leu Leu Pro Cys Phe Leu Glu His Asn Met Lys Arg 
    50                  55                  60                  


Asp Glu Asp Pro His Lys Ala Ala Lys Glu Met Pro Phe Gln Gly Ser 
65                  70                  75                  80  


Gly Lys Ser Ala Trp Cys Pro Val Glu Ile Ser Lys Thr Val Leu Trp 
                85                  90                  95      


Pro Glu Ser Ile Ser Val Val Arg Cys Val Glu Leu Phe Glu Ala Pro 
            100                 105                 110         


Val Glu Cys Glu Glu Glu Glu Glu Val Glu Glu Glu Lys Gly Ser Phe 
        115                 120                 125             


Cys Ala Ser Pro Glu Ser Ser Arg Asp Asp Phe Gln Glu Gly Arg Glu 
    130                 135                 140                 


Gly Ile Val Ala Arg Leu Thr Glu Ser Leu Phe Leu Asp Leu Leu Gly 
145                 150                 155                 160 


Glu Glu Asn Gly Gly Phe Cys Gln Gln Asp Met Gly Glu Ser Cys Leu 
                165                 170                 175     


Leu Pro Pro Ser Gly Ser Thr Ser Ala His Met Pro Trp Asp Glu Phe 
            180                 185                 190         


Pro Ser Ala Gly Pro Lys Glu Ala Pro Pro Trp Gly Lys Glu Gln Pro 
        195                 200                 205             


Leu His Leu Glu Pro Ser Pro Pro Ala Ser Pro Thr Gln Ser Pro Asp 
    210                 215                 220                 


Asn Leu Thr Cys Thr Glu Thr Pro Leu Val Ile Ala Gly Asn Pro Ala 
225                 230                 235                 240 


Tyr Arg Ser Phe Ser Asn Ser Leu Ser Gln Ser Pro Cys Pro Arg Glu 
                245                 250                 255     


Leu Gly Pro Asp Pro Leu Leu Ala Arg His Leu Glu Glu Val Glu Pro 
            260                 265                 270         


Glu Met Pro Cys Val Pro Gln Leu Ser Glu Pro Thr Thr Val Pro Gln 
        275                 280                 285             


Pro Glu Pro Glu Thr Trp Glu Gln Ile Leu Arg Arg Asn Val Leu Gln 
    290                 295                 300                 


His Gly Ala Ala Ala Ala Pro Val Ser Ala Pro Thr Ser Gly Tyr Gln 
305                 310                 315                 320 


Glu Phe Val His Ala Val Glu Gln Gly Gly Thr Gln Ala Ser Ala Val 
                325                 330                 335     


Val Gly Leu Gly Pro Pro Gly Glu Ala Gly Tyr Lys Ala Phe Ser Ser 
            340                 345                 350         


Leu Leu Ala Ser Ser Ala Val Ser Pro Glu Lys Cys Gly Phe Gly Ala 
        355                 360                 365             


Ser Ser Gly Glu Glu Gly Tyr Lys Pro Phe Gln Asp Leu Ile Pro Gly 
    370                 375                 380                 


Cys Pro Gly Asp Pro Ala Pro Val Pro Val Pro Leu Phe Thr Phe Gly 
385                 390                 395                 400 


Leu Asp Arg Glu Pro Pro Arg Ser Pro Gln Ser Ser His Leu Pro Ser 
                405                 410                 415     


Ser Ser Pro Glu His Leu Gly Leu Glu Pro Gly Glu Lys Val Glu Asp 
            420                 425                 430         


Met Pro Lys Pro Pro Leu Pro Gln Glu Gln Ala Thr Asp Pro Leu Val 
        435                 440                 445             


Asp Ser Leu Gly Ser Gly Ile Val Tyr Ser Ala Leu Thr Cys His Leu 
    450                 455                 460                 


Cys Gly His Leu Lys Gln Cys His Gly Gln Glu Asp Gly Gly Gln Thr 
465                 470                 475                 480 


Pro Val Met Ala Ser Pro Cys Cys Gly Cys Cys Cys Gly Asp Arg Ser 
                485                 490                 495     


Ser Pro Pro Thr Thr Pro Leu Arg Ala Pro Asp Pro Ser Pro Gly Gly 
            500                 505                 510         


Val Pro Leu Glu Ala Ser Leu Cys Pro Ala Ser Leu Ala Pro Ser Gly 
        515                 520                 525             


Ile Ser Glu Lys Ser Lys Ser Ser Ser Ser Phe His Pro Ala Pro Gly 
    530                 535                 540                 


Asn Ala Gln Ser Ser Ser Gln Thr Pro Lys Ile Val Asn Phe Val Ser 
545                 550                 555                 560 


Val Gly Pro Thr Tyr Met Arg Val Ser 
                565                 


<210>  18
<211>  437
<212>  PRT
<213>  Homo sapiens

<400>  18

Arg Phe Cys Gly Ile Tyr Gly Tyr Arg Leu Arg Arg Lys Trp Glu Glu 
1               5                   10                  15      


Lys Ile Pro Asn Pro Ser Lys Ser His Leu Phe Gln Asn Gly Ser Ala 
            20                  25                  30          


Glu Leu Trp Pro Pro Gly Ser Met Ser Ala Phe Thr Ser Gly Ser Pro 
        35                  40                  45              


Pro His Gln Gly Pro Trp Gly Ser Arg Phe Pro Glu Leu Glu Gly Val 
    50                  55                  60                  


Phe Pro Val Gly Phe Gly Asp Ser Glu Val Ser Pro Leu Thr Ile Glu 
65                  70                  75                  80  


Asp Pro Lys His Val Cys Asp Pro Pro Ser Gly Pro Asp Thr Thr Pro 
                85                  90                  95      


Ala Ala Ser Asp Leu Pro Thr Glu Gln Pro Pro Ser Pro Gln Pro Gly 
            100                 105                 110         


Pro Pro Ala Ala Ser His Thr Pro Glu Lys Gln Ala Ser Ser Phe Asp 
        115                 120                 125             


Phe Asn Gly Pro Tyr Leu Gly Pro Pro His Ser Arg Ser Leu Pro Asp 
    130                 135                 140                 


Ile Leu Gly Gln Pro Glu Pro Pro Gln Glu Gly Gly Ser Gln Lys Ser 
145                 150                 155                 160 


Pro Pro Pro Gly Ser Leu Glu Tyr Leu Cys Leu Pro Ala Gly Gly Gln 
                165                 170                 175     


Val Gln Leu Val Pro Leu Ala Gln Ala Met Gly Pro Gly Gln Ala Val 
            180                 185                 190         


Glu Val Glu Arg Arg Pro Ser Gln Gly Ala Ala Gly Ser Pro Ser Leu 
        195                 200                 205             


Glu Ser Gly Gly Gly Pro Ala Pro Pro Ala Leu Gly Pro Arg Val Gly 
    210                 215                 220                 


Gly Gln Asp Gln Lys Asp Ser Pro Val Ala Ile Pro Met Ser Ser Gly 
225                 230                 235                 240 


Asp Thr Glu Asp Pro Gly Val Ala Ser Gly Tyr Val Ser Ser Ala Asp 
                245                 250                 255     


Leu Val Phe Thr Pro Asn Ser Gly Ala Ser Ser Val Ser Leu Val Pro 
            260                 265                 270         


Ser Leu Gly Leu Pro Ser Asp Gln Thr Pro Ser Leu Cys Pro Gly Leu 
        275                 280                 285             


Ala Ser Gly Pro Pro Gly Ala Pro Gly Pro Val Lys Ser Gly Phe Glu 
    290                 295                 300                 


Gly Tyr Val Glu Leu Pro Pro Ile Glu Gly Arg Ser Pro Arg Ser Pro 
305                 310                 315                 320 


Arg Asn Asn Pro Val Pro Pro Glu Ala Lys Ser Pro Val Leu Asn Pro 
                325                 330                 335     


Gly Glu Arg Pro Ala Asp Val Ser Pro Thr Ser Pro Gln Pro Glu Gly 
            340                 345                 350         


Leu Leu Val Leu Gln Gln Val Gly Asp Tyr Cys Phe Leu Pro Gly Leu 
        355                 360                 365             


Gly Pro Gly Pro Leu Ser Leu Arg Ser Lys Pro Ser Ser Pro Gly Pro 
    370                 375                 380                 


Gly Pro Glu Ile Lys Asn Leu Asp Gln Ala Phe Gln Val Lys Lys Pro 
385                 390                 395                 400 


Pro Gly Gln Ala Val Pro Gln Val Pro Val Ile Gln Leu Phe Lys Ala 
                405                 410                 415     


Leu Lys Gln Gln Asp Tyr Leu Ser Leu Pro Pro Trp Glu Val Asn Lys 
            420                 425                 430         


Pro Gly Glu Val Cys 
        435         


<210>  19
<211>  189
<212>  PRT
<213>  Homo sapiens

<400>  19

Arg His Glu Arg Ile Lys Lys Thr Ser Phe Ser Thr Thr Thr Leu Leu 
1               5                   10                  15      


Pro Pro Ile Lys Val Leu Val Val Tyr Pro Ser Glu Ile Cys Phe His 
            20                  25                  30          


His Thr Ile Cys Tyr Phe Thr Glu Phe Leu Gln Asn His Cys Arg Ser 
        35                  40                  45              


Glu Val Ile Leu Glu Lys Trp Gln Lys Lys Lys Ile Ala Glu Met Gly 
    50                  55                  60                  


Pro Val Gln Trp Leu Ala Thr Gln Lys Lys Ala Ala Asp Lys Val Val 
65                  70                  75                  80  


Phe Leu Leu Ser Asn Asp Val Asn Ser Val Cys Asp Gly Thr Cys Gly 
                85                  90                  95      


Lys Ser Glu Gly Ser Pro Ser Glu Asn Ser Gln Asp Leu Phe Pro Leu 
            100                 105                 110         


Ala Phe Asn Leu Phe Cys Ser Asp Leu Arg Ser Gln Ile His Leu His 
        115                 120                 125             


Lys Tyr Val Val Val Tyr Phe Arg Glu Ile Asp Thr Lys Asp Asp Tyr 
    130                 135                 140                 


Asn Ala Leu Ser Val Cys Pro Lys Tyr His Leu Met Lys Asp Ala Thr 
145                 150                 155                 160 


Ala Phe Cys Ala Glu Leu Leu His Val Lys Gln Gln Val Ser Ala Gly 
                165                 170                 175     


Lys Arg Ser Gln Ala Cys His Asp Gly Cys Cys Ser Leu 
            180                 185                 


<210>  20
<211>  91
<212>  PRT
<213>  Homo sapiens

<400>  20

Lys Lys Arg Ile Lys Pro Ile Val Trp Pro Ser Leu Pro Asp His Lys 
1               5                   10                  15      


Lys Thr Leu Glu His Leu Cys Lys Lys Pro Arg Lys Asn Leu Asn Val 
            20                  25                  30          


Ser Phe Asn Pro Glu Ser Phe Leu Asp Cys Gln Ile His Arg Val Asp 
        35                  40                  45              


Asp Ile Gln Ala Arg Asp Glu Val Glu Gly Phe Leu Gln Asp Thr Phe 
    50                  55                  60                  


Pro Gln Gln Pro Ile Leu Thr Ser Leu Gly Ser Asn Gln Glu Glu Ala 
65                  70                  75                  80  


Tyr Val Thr Met Ser Ser Phe Tyr Gln Asn Gln 
                85                  90      


<210>  21
<211>  57
<212>  PRT
<213>  Homo sapiens

<400>  21

Lys Val Leu Lys Cys Asn Thr Pro Asp Pro Ser Lys Phe Phe Ser Gln 
1               5                   10                  15      


Leu Ser Ser Glu His Gly Gly Asp Val Gln Lys Trp Leu Ser Ser Pro 
            20                  25                  30          


Phe Pro Ser Ser Ser Phe Ser Pro Gly Gly Leu Ala Pro Glu Ile Ser 
        35                  40                  45              


Pro Leu Glu Val Leu Glu Arg Asp Lys 
    50                  55          


<210>  22
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  22

Asn Pro Trp Phe Gln Arg Ala Lys Met Pro Arg Ala Leu Asp Phe Ser 
1               5                   10                  15      


Gly His Thr His Pro Val Ala Thr Phe Gln Pro Ser Arg Pro Glu Ser 
            20                  25                  30          


Val Asn Asp Leu Phe Leu Cys Pro Gln Lys Glu Leu Thr 
        35                  40                  45  


<210>  23
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  23

Gly Tyr Ile Cys Leu Arg Asn Ser Leu Pro Lys Val Leu Asn Phe His 
1               5                   10                  15      


Asn Phe Leu Ala Trp Pro Phe Pro Asn Leu Pro Pro Leu Glu Ala Met 
            20                  25                  30          


Asp Met Val Glu Val Ile Tyr Ile Asn Arg 
        35                  40          


<210>  24
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  24

Pro Leu Lys Glu Lys Ser Ile Ile Leu Pro Lys Ser Leu Ile Ser Val 
1               5                   10                  15      


Val Arg Ser Ala Thr Leu Glu Thr Lys Pro Glu Ser Lys Tyr Val Ser 
            20                  25                  30          


Leu Ile Thr Ser Tyr Gln Pro Phe Ser Leu 
        35                  40          


<210>  25
<211>  43
<212>  PRT
<213>  Homo sapiens

<400>  25

Arg Arg Arg Lys Lys Leu Pro Ser Val Leu Leu Phe Lys Lys Pro Ser 
1               5                   10                  15      


Pro Phe Ile Phe Ile Ser Gln Arg Pro Ser Pro Glu Thr Gln Asp Thr 
            20                  25                  30          


Ile His Pro Leu Asp Glu Glu Ala Phe Leu Lys 
        35                  40              


<210>  26
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  26

Tyr Ile His Val Gly Lys Glu Lys His Pro Ala Asn Leu Ile Leu Ile 
1               5                   10                  15      


Tyr Gly Asn Glu Phe Asp Lys Arg Phe Phe Val Pro Ala Glu Lys Ile 
            20                  25                  30          


Val Ile Asn Phe Ile Thr Leu Asn Ile Ser Asp Asp Ser 
        35                  40                  45  


<210>  27
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  27

Arg Tyr Val Thr Lys Pro Pro Ala Pro Pro Asn Ser Leu Asn Val Gln 
1               5                   10                  15      


Arg Val Leu Thr Phe Gln Pro Leu Arg Phe Ile Gln Glu His Val Leu 
            20                  25                  30          


Ile Pro Val Phe Asp Leu Ser Gly Pro 
        35                  40      


<210>  28
<211>  43
<212>  PRT
<213>  Homo sapiens

<400>  28

Asn Tyr Val Phe Phe Pro Ser Leu Lys Pro Ser Ser Ser Ile Asp Glu 
1               5                   10                  15      


Tyr Phe Ser Glu Gln Pro Leu Lys Asn Leu Leu Leu Ser Thr Ser Glu 
            20                  25                  30          


Glu Gln Ile Glu Lys Cys Phe Ile Ile Glu Asn 
        35                  40              


<210>  29
<211>  49
<212>  PRT
<213>  Homo sapiens

<400>  29

Tyr Trp Phe His Thr Pro Pro Ser Ile Pro Leu Gln Ile Glu Glu Tyr 
1               5                   10                  15      


Leu Lys Asp Pro Thr Gln Pro Ile Leu Glu Ala Leu Asp Lys Asp Ser 
            20                  25                  30          


Ser Pro Lys Asp Asp Val Trp Asp Ser Val Ser Ile Ile Ser Phe Pro 
        35                  40                  45              


Glu 
    


<210>  30
<211>  51
<212>  PRT
<213>  Homo sapiens

<400>  30

Tyr Ala Phe Ser Pro Arg Asn Ser Leu Pro Gln His Leu Lys Glu Phe 
1               5                   10                  15      


Leu Gly His Pro His His Asn Thr Leu Leu Phe Phe Ser Phe Pro Leu 
            20                  25                  30          


Ser Asp Glu Asn Asp Val Phe Asp Lys Leu Ser Val Ile Ala Glu Asp 
        35                  40                  45              


Ser Glu Ser 
    50      


<210>  31
<211>  41
<212>  PRT
<213>  Homo sapiens

<400>  31

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  32
<211>  364
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2 LBD Chimeric receptor

<400>  32

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro His Asn Thr Thr Val Phe Gln Gly Val Ala Gly 
            20                  25                  30          


Gln Ser Leu Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly 
        35                  40                  45              


Arg Arg Lys Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln 
    50                  55                  60                  


Arg Val Val Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg 
65                  70                  75                  80  


Trp Asn Gly Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu 
                85                  90                  95      


Thr Ile Thr Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln 
            100                 105                 110         


Cys Gln Ser Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu 
        115                 120                 125             


Val Glu Val Leu Ala Asp Pro Leu Asp His Arg Asp Ala Ile Glu Val 
    130                 135                 140                 


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys Ser 
145                 150                 155                 160 


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
                165                 170                 175     


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
            180                 185                 190         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        195                 200                 205             


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
    210                 215                 220                 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
225                 230                 235                 240 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
                245                 250                 255     


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
            260                 265                 270         


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
        275                 280                 285             


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
    290                 295                 300                 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
305                 310                 315                 320 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
                325                 330                 335     


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
            340                 345                 350         


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        355                 360                 


<210>  33
<211>  364
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2 mutant LBD chimeric receptor

<400>  33

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro His Asn Thr Thr Val Phe Gln Gly Val Ala Gly 
            20                  25                  30          


Gln Ser Leu Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly 
        35                  40                  45              


Arg Arg Lys Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln 
    50                  55                  60                  


Arg Val Val Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg 
65                  70                  75                  80  


Trp Asn Gly Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu 
                85                  90                  95      


Thr Ile Lys Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln 
            100                 105                 110         


Cys Gln Ser Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu 
        115                 120                 125             


Val Glu Val Leu Ala Asp Pro Leu Asp His Arg Asp Ala Ile Glu Val 
    130                 135                 140                 


Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Leu Asp Asn Glu Lys Ser 
145                 150                 155                 160 


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
                165                 170                 175     


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
            180                 185                 190         


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
        195                 200                 205             


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
    210                 215                 220                 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
225                 230                 235                 240 


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe 
                245                 250                 255     


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu 
            260                 265                 270         


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
        275                 280                 285             


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
    290                 295                 300                 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
305                 310                 315                 320 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
                325                 330                 335     


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
            340                 345                 350         


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        355                 360                 


<210>  34
<211>  1092
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TREM2 LBD chimeric receptor

<400>  34
atggccctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgcccgg       60

cctcacaaca ccacagtgtt ccagggcgtg gcgggccagt ccctgcaggt gtcttgcccc      120

tatgactcca tgaagcactg ggggaggcgc aaggcctggt gccgccagct gggagagaag      180

ggcccatgcc agcgtgtggt cagcacgcac aacttgtggc tgctgtcctt cctgaggagg      240

tggaatggga gcacagccat cacagacgat accctgggtg gcactctcac cattacgctg      300

cggaatctac aaccccatga tgcgggtctc taccagtgcc agagcctcca tggcagtgag      360

gctgacaccc tcaggaaggt cctggtggag gtgctggcag accccctgga tcaccgggat      420

gctatcgagg tggagcagaa gctgatcagc gaggaggacc tgctggacaa cgagaagagc      480

aacggcacca tcatccacgt gaagggcaag cacctgtgcc ccagccccct gttccccggc      540

cccagcaagc ccttctgggt gctggtggtg gtgggcggcg tgctggcctg ctacagcctg      600

ctggtgaccg tggccttcat catcttctgg gtgcggagca agcggagccg gctgctgcac      660

agcgactaca tgaacatgac cccccggcgg cctgggccca cccgcaagca ttaccagccc      720

tatgccccac cacgcgactt cgcagcctat cgctccagag tgaagttcag caggagcgca      780

gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa tctaggacga      840

agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag      900

ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg      960

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc     1020

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc     1080

ctgccccctc gc                                                         1092


<210>  35
<211>  1092
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TREM2 mutant LBD chimeric receptor

<400>  35
atggccctgc ccgtgaccgc cctgctgctg cccctggccc tgctgctgca cgccgcccgg       60

cctcacaaca ccacagtgtt ccagggcgtg gcgggccagt ccctgcaggt gtcttgcccc      120

tatgactcca tgaagcactg ggggaggcgc aaggcctggt gccgccagct gggagagaag      180

ggcccatgcc agcgtgtggt cagcacgcac aacttgtggc tgctgtcctt cctgaggagg      240

tggaatggga gcacagccat cacagacgat accctgggtg gcactctcac cattaagctg      300

cggaatctac aaccccatga tgcgggtctc taccagtgcc agagcctcca tggcagtgag      360

gctgacaccc tcaggaaggt cctggtggag gtgctggcag accccctgga tcaccgggat      420

gctatcgagg tggagcagaa gctgatcagc gaggaggacc tgctggacaa cgagaagagc      480

aacggcacca tcatccacgt gaagggcaag cacctgtgcc ccagccccct gttccccggc      540

cccagcaagc ccttctgggt gctggtggtg gtgggcggcg tgctggcctg ctacagcctg      600

ctggtgaccg tggccttcat catcttctgg gtgcggagca agcggagccg gctgctgcac      660

agcgactaca tgaacatgac cccccggcgg cctgggccca cccgcaagca ttaccagccc      720

tatgccccac cacgcgactt cgcagcctat cgctccagag tgaagttcag caggagcgca      780

gacgcccccg cgtaccagca gggccagaac cagctctata acgagctcaa tctaggacga      840

agagaggagt acgatgtttt ggacaagaga cgtggccggg accctgagat ggggggaaag      900

ccgagaagga agaaccctca ggaaggcctg tacaatgaac tgcagaaaga taagatggcg      960

gaggcctaca gtgagattgg gatgaaaggc gagcgccgga ggggcaaggg gcacgatggc     1020

ctttaccagg gtctcagtac agccaccaag gacacctacg acgcccttca catgcaggcc     1080

ctgccccctc gc                                                         1092


<210>  36
<211>  156
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant ligand binding domain of TREM2


<220>
<221>  VARIANT
<222>  (118)..(119)
<223>  X at position 118 is not R and/or X at position 119 is not D

<220>
<221>  VARIANT
<222>  (139)..(140)
<223>  X at position 139 is not H and/or X at position 140 is not S

<400>  36

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Xaa Xaa Ala Gly Asp Leu Trp Phe Pro Gly Glu 
        115                 120                 125             


Ser Glu Ser Phe Glu Asp Ala His Val Glu Xaa Xaa Ile Ser Arg Ser 
    130                 135                 140                 


Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser 
145                 150                 155     


<210>  37
<211>  156
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Variant ligand binding domain of TREM2


<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  X is a basic amino acid, preferably K or R

<220>
<221>  VARIANT
<222>  (118)..(119)
<223>  X at position 118 is not R and/or X at position 119 is not D

<220>
<221>  VARIANT
<222>  (139)..(140)
<223>  X at position 139 is not H and/or X at position 140 is not S

<400>  37

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Xaa Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Xaa Xaa Ala Gly Asp Leu Trp Phe Pro Gly Glu 
        115                 120                 125             


Ser Glu Ser Phe Glu Asp Ala His Val Glu Xaa Xaa Ile Ser Arg Ser 
    130                 135                 140                 


Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser 
145                 150                 155     


<210>  38
<211>  52
<212>  PRT
<213>  Homo sapiens

<400>  38

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile 
    50          


<210>  39
<211>  86
<212>  PRT
<213>  Homo sapiens

<400>  39

Glu Arg Thr Met Pro Arg Ile Pro Thr Leu Lys Asn Leu Glu Asp Leu 
1               5                   10                  15      


Val Thr Glu Tyr His Gly Asn Phe Ser Ala Trp Ser Gly Val Ser Lys 
            20                  25                  30          


Gly Leu Ala Glu Ser Leu Gln Pro Asp Tyr Ser Glu Arg Leu Cys Leu 
        35                  40                  45              


Val Ser Glu Ile Pro Pro Lys Gly Gly Ala Leu Gly Glu Gly Pro Gly 
    50                  55                  60                  


Ala Ser Pro Cys Asn Gln His Ser Pro Tyr Trp Ala Pro Pro Cys Tyr 
65                  70                  75                  80  


Thr Leu Lys Pro Glu Thr 
                85      


<210>  40
<211>  355
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD28-CD3zeta CAR

<400>  40

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro His Asn Thr Thr Val Phe Gln Gly Val Ala Gly 
            20                  25                  30          


Gln Ser Leu Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly 
        35                  40                  45              


Arg Arg Lys Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln 
    50                  55                  60                  


Arg Val Val Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg 
65                  70                  75                  80  


Trp Asn Gly Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu 
                85                  90                  95      


Thr Ile Thr Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln 
            100                 105                 110         


Cys Gln Ser Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu 
        115                 120                 125             


Val Glu Val Leu Ala Asp Pro Leu Asp His Arg Asp Ala Ile Glu Val 
    130                 135                 140                 


Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys 
145                 150                 155                 160 


His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp 
                165                 170                 175     


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
            180                 185                 190         


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
        195                 200                 205             


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
    210                 215                 220                 


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
225                 230                 235                 240 


Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                245                 250                 255     


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            260                 265                 270         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        275                 280                 285             


Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    290                 295                 300                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
305                 310                 315                 320 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                325                 330                 335     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            340                 345                 350         


Pro Pro Arg 
        355 


<210>  41
<211>  378
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD8-CD28-CD3zeta CAR

<400>  41

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro His Asn Thr Thr Val Phe Gln Gly Val Ala Gly 
            20                  25                  30          


Gln Ser Leu Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly 
        35                  40                  45              


Arg Arg Lys Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln 
    50                  55                  60                  


Arg Val Val Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg 
65                  70                  75                  80  


Trp Asn Gly Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu 
                85                  90                  95      


Thr Ile Thr Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln 
            100                 105                 110         


Cys Gln Ser Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu 
        115                 120                 125             


Val Glu Val Leu Ala Asp Pro Leu Asp His Arg Asp Ala Phe Val Pro 
    130                 135                 140                 


Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro 
145                 150                 155                 160 


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
                165                 170                 175     


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
            180                 185                 190         


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
        195                 200                 205             


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
    210                 215                 220                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
225                 230                 235                 240 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                245                 250                 255     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
            260                 265                 270         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        275                 280                 285             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    290                 295                 300                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro 
305                 310                 315                 320 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                325                 330                 335     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            340                 345                 350         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        355                 360                 365             


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    370                 375             


<210>  42
<211>  355
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2mut-CD28-CD3zeta CAR

<400>  42

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro His Asn Thr Thr Val Phe Gln Gly Val Ala Gly 
            20                  25                  30          


Gln Ser Leu Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly 
        35                  40                  45              


Arg Arg Lys Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln 
    50                  55                  60                  


Arg Val Val Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg 
65                  70                  75                  80  


Trp Asn Gly Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu 
                85                  90                  95      


Thr Ile Lys Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln 
            100                 105                 110         


Cys Gln Ser Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu 
        115                 120                 125             


Val Glu Val Leu Ala Asp Pro Leu Asp His Arg Asp Ala Ile Glu Val 
    130                 135                 140                 


Leu Asp Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys 
145                 150                 155                 160 


His Leu Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp 
                165                 170                 175     


Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
            180                 185                 190         


Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
        195                 200                 205             


Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
    210                 215                 220                 


Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
225                 230                 235                 240 


Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
                245                 250                 255     


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            260                 265                 270         


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        275                 280                 285             


Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
    290                 295                 300                 


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
305                 310                 315                 320 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
                325                 330                 335     


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
            340                 345                 350         


Pro Pro Arg 
        355 


<210>  43
<211>  378
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2mut-CD8-CD28-CD3zeta CAR

<400>  43

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro His Asn Thr Thr Val Phe Gln Gly Val Ala Gly 
            20                  25                  30          


Gln Ser Leu Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly 
        35                  40                  45              


Arg Arg Lys Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln 
    50                  55                  60                  


Arg Val Val Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg 
65                  70                  75                  80  


Trp Asn Gly Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu 
                85                  90                  95      


Thr Ile Lys Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln 
            100                 105                 110         


Cys Gln Ser Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu 
        115                 120                 125             


Val Glu Val Leu Ala Asp Pro Leu Asp His Arg Asp Ala Phe Val Pro 
    130                 135                 140                 


Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro 
145                 150                 155                 160 


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
                165                 170                 175     


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
            180                 185                 190         


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
        195                 200                 205             


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg Asn 
    210                 215                 220                 


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
225                 230                 235                 240 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
                245                 250                 255     


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
            260                 265                 270         


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
        275                 280                 285             


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
    290                 295                 300                 


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro 
305                 310                 315                 320 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                325                 330                 335     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            340                 345                 350         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        355                 360                 365             


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    370                 375             


<210>  44
<211>  460
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD3zeta-2A-DAP10 CAR

<400>  44

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Ile His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala Ala 
    370                 375                 380                 


Ala Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr Pro 
385                 390                 395                 400 


Gly Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro Leu 
                405                 410                 415     


Leu Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile Val 
            420                 425                 430         


Gly Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu 
        435                 440                 445             


Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly 
    450                 455                 460 


<210>  45
<211>  501
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD3zeta-2A-DAP10-CD28 CAR

<400>  45

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Ile His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala Ala 
    370                 375                 380                 


Ala Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr Pro 
385                 390                 395                 400 


Gly Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro Leu 
                405                 410                 415     


Leu Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile Val 
            420                 425                 430         


Gly Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu 
        435                 440                 445             


Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly Arg Ser Lys Arg 
    450                 455                 460                 


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
465                 470                 475                 480 


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
                485                 490                 495     


Ala Ala Tyr Arg Ser 
            500     


<210>  46
<211>  408
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD28-2A-DAP12 CAR

<400>  46

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
225                 230                 235                 240 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
                245                 250                 255     


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Arg Ala Lys 
            260                 265                 270         


Arg Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Ile Thr Cys Gly Asp 
        275                 280                 285             


Val Glu Glu Asn Pro Gly Pro Met Gly Gly Leu Glu Pro Cys Ser Arg 
    290                 295                 300                 


Leu Leu Leu Leu Pro Leu Leu Leu Ala Val Ser Gly Leu Arg Pro Val 
305                 310                 315                 320 


Gln Ala Gln Ala Gln Ser Asp Cys Ser Cys Ser Thr Val Ser Pro Gly 
                325                 330                 335     


Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu Ile 
            340                 345                 350         


Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly 
        355                 360                 365             


Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser 
    370                 375                 380                 


Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu 
385                 390                 395                 400 


Asn Thr Gln Arg Pro Tyr Tyr Lys 
                405             


<210>  47
<211>  521
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD28-2A-DAP12-CD3zeta CAR

<400>  47

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
225                 230                 235                 240 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
                245                 250                 255     


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Arg Ala Lys 
            260                 265                 270         


Arg Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Ile Thr Cys Gly Asp 
        275                 280                 285             


Val Glu Glu Asn Pro Gly Pro Met Gly Gly Leu Glu Pro Cys Ser Arg 
    290                 295                 300                 


Leu Leu Leu Leu Pro Leu Leu Leu Ala Val Ser Gly Leu Arg Pro Val 
305                 310                 315                 320 


Gln Ala Gln Ala Gln Ser Asp Cys Ser Cys Ser Thr Val Ser Pro Gly 
                325                 330                 335     


Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu Ile 
            340                 345                 350         


Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly 
        355                 360                 365             


Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser 
    370                 375                 380                 


Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu 
385                 390                 395                 400 


Asn Thr Gln Arg Pro Tyr Tyr Lys Arg Val Lys Phe Ser Arg Ser Ala 
                405                 410                 415     


Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
            420                 425                 430         


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
        435                 440                 445             


Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln 
    450                 455                 460                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
465                 470                 475                 480 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                485                 490                 495     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            500                 505                 510         


Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520     


<210>  48
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-CD3zeta-2A-DAP12trunc-CD28 CAR

<400>  48

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Gly Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu 
    370                 375                 380                 


Ala Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys 
385                 390                 395                 400 


Ser Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly 
                405                 410                 415     


Asp Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly 
            420                 425                 430         


Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Ser 
        435                 440                 445             


Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg 
    450                 455                 460                 


Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg 
465                 470                 475                 480 


Asp Phe Ala Ala Tyr Arg Ser 
                485         


<210>  49
<211>  333
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-2A-DAP12trunc CAR

<400>  49

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
                245                 250                 255     


Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala 
            260                 265                 270         


Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys Ser 
        275                 280                 285             


Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly Asp 
    290                 295                 300                 


Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg 
305                 310                 315                 320 


Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr 
                325                 330             


<210>  50
<211>  460
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2mut-CD3zeta-2A-DAP10 CAR

<400>  50

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Lys 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Ile His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala Ala 
    370                 375                 380                 


Ala Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr Pro 
385                 390                 395                 400 


Gly Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro Leu 
                405                 410                 415     


Leu Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile Val 
            420                 425                 430         


Gly Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu 
        435                 440                 445             


Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly 
    450                 455                 460 


<210>  51
<211>  408
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2mut-CD28-2A-DAP12 CAR

<400>  51

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Lys 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
225                 230                 235                 240 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
                245                 250                 255     


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Arg Ala Lys 
            260                 265                 270         


Arg Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Ile Thr Cys Gly Asp 
        275                 280                 285             


Val Glu Glu Asn Pro Gly Pro Met Gly Gly Leu Glu Pro Cys Ser Arg 
    290                 295                 300                 


Leu Leu Leu Leu Pro Leu Leu Leu Ala Val Ser Gly Leu Arg Pro Val 
305                 310                 315                 320 


Gln Ala Gln Ala Gln Ser Asp Cys Ser Cys Ser Thr Val Ser Pro Gly 
                325                 330                 335     


Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu Ile 
            340                 345                 350         


Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly 
        355                 360                 365             


Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser 
    370                 375                 380                 


Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu 
385                 390                 395                 400 


Asn Thr Gln Arg Pro Tyr Tyr Lys 
                405             


<210>  52
<211>  408
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-2A-DAP12-CD28 CAR

<400>  52

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
                245                 250                 255     


Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala 
            260                 265                 270         


Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys Ser 
        275                 280                 285             


Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly Asp 
    290                 295                 300                 


Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg 
305                 310                 315                 320 


Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Lys Gln 
                325                 330                 335     


Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln Gly Gln Arg 
            340                 345                 350         


Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr Tyr Lys Arg 
        355                 360                 365             


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
    370                 375                 380                 


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
385                 390                 395                 400 


Arg Asp Phe Ala Ala Tyr Arg Ser 
                405             


<210>  53
<211>  460
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-2A-DAP10-CD3zeta CAR

<400>  53

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Ile 
                245                 250                 255     


His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala Ala Ala 
            260                 265                 270         


Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr Pro Gly 
        275                 280                 285             


Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro Leu Leu 
    290                 295                 300                 


Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile Val Gly 
305                 310                 315                 320 


Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu Asp 
                325                 330                 335     


Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly Arg Val Lys Phe Ser 
            340                 345                 350         


Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr 
        355                 360                 365             


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
    370                 375                 380                 


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys 
385                 390                 395                 400 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
                405                 410                 415     


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
            420                 425                 430         


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
        435                 440                 445             


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    450                 455                 460 


<210>  54
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-2A-DAP12trunc-CD28-CD3zeta CAR

<400>  54

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
                245                 250                 255     


Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala 
            260                 265                 270         


Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys Ser 
        275                 280                 285             


Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly Asp 
    290                 295                 300                 


Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg 
305                 310                 315                 320 


Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly 
            420                 425                 430         


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
        435                 440                 445             


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    450                 455                 460                 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
465                 470                 475                 480 


Met Gln Ala Leu Pro Pro Arg 
                485         


<210>  55
<211>  487
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2mut-2A-DAP12tr-CD28-CD3zeta CAR

<400>  55

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Lys 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
                245                 250                 255     


Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala 
            260                 265                 270         


Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys Ser 
        275                 280                 285             


Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly Asp 
    290                 295                 300                 


Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg 
305                 310                 315                 320 


Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly 
            420                 425                 430         


Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu 
        435                 440                 445             


Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu 
    450                 455                 460                 


Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His 
465                 470                 475                 480 


Met Gln Ala Leu Pro Pro Arg 
                485         


<210>  56
<211>  425
<212>  PRT
<213>  Artificial sequence

<220>
<223>  TREM2-2A-DAP12tr-CD28-CD3tr CAR

<400>  56

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Lys 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
                245                 250                 255     


Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala 
            260                 265                 270         


Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys Ser 
        275                 280                 285             


Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly Asp 
    290                 295                 300                 


Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg 
305                 310                 315                 320 


Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr Arg Ser Lys 
                325                 330                 335     


Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg 
            340                 345                 350         


Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp 
        355                 360                 365             


Phe Ala Ala Tyr Arg Ser Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
    370                 375                 380                 


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
385                 390                 395                 400 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
                405                 410                 415     


Asp Thr Tyr Asp Ala Leu His Met Gln 
            420                 425 


<210>  57
<211>  460
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TREM2-CD3z-2A-DAP10_2A-GFP

<400>  57

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Ile His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala Ala 
    370                 375                 380                 


Ala Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr Pro 
385                 390                 395                 400 


Gly Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro Leu 
                405                 410                 415     


Leu Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile Val 
            420                 425                 430         


Gly Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu 
        435                 440                 445             


Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly 
    450                 455                 460 


<210>  58
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TREM2-CD3z-2A-DAP10-CD28_2A_GFP

<400>  58

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Ile His Leu Gly His Ile Leu Phe Leu Leu Leu Leu Pro Val Ala Ala 
    370                 375                 380                 


Ala Gln Thr Thr Pro Gly Glu Arg Ser Ser Leu Pro Ala Phe Tyr Pro 
385                 390                 395                 400 


Gly Thr Ser Gly Ser Cys Ser Gly Cys Gly Ser Leu Ser Leu Pro Leu 
                405                 410                 415     


Leu Ala Gly Leu Val Ala Ala Asp Ala Val Ala Ser Leu Leu Ile Val 
            420                 425                 430         


Gly Ala Val Phe Leu Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu 
        435                 440                 445             


Asp Gly Lys Val Tyr Ile Asn Met Pro Gly Arg Gly Arg Ser Lys Arg 
    450                 455                 460                 


Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro 
465                 470                 475                 480 


Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe 
                485                 490                 495     


Ala Ala Tyr Arg Ser 
            500     


<210>  59
<211>  408
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TREM2-CD28-2A-DAP12_2A_GFP

<400>  59

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
225                 230                 235                 240 


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
                245                 250                 255     


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Ala Arg Ala Lys 
            260                 265                 270         


Arg Ser Gly Ser Gly Glu Gly Arg Gly Ser Leu Ile Thr Cys Gly Asp 
        275                 280                 285             


Val Glu Glu Asn Pro Gly Pro Met Gly Gly Leu Glu Pro Cys Ser Arg 
    290                 295                 300                 


Leu Leu Leu Leu Pro Leu Leu Leu Ala Val Ser Gly Leu Arg Pro Val 
305                 310                 315                 320 


Gln Ala Gln Ala Gln Ser Asp Cys Ser Cys Ser Thr Val Ser Pro Gly 
                325                 330                 335     


Val Leu Ala Gly Ile Val Met Gly Asp Leu Val Leu Thr Val Leu Ile 
            340                 345                 350         


Ala Leu Ala Val Tyr Phe Leu Gly Arg Leu Val Pro Arg Gly Arg Gly 
        355                 360                 365             


Ala Ala Glu Ala Ala Thr Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser 
    370                 375                 380                 


Pro Tyr Gln Glu Leu Gln Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu 
385                 390                 395                 400 


Asn Thr Gln Arg Pro Tyr Tyr Lys 
                405             


<210>  60
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TREM2-CD3z-2A-DAP12trunc-CD28_2A_GFP

<400>  60

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
225                 230                 235                 240 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                245                 250                 255     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            260                 265                 270         


Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn 
        275                 280                 285             


Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
    290                 295                 300                 


Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly 
305                 310                 315                 320 


Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala 
                325                 330                 335     


Leu Pro Pro Arg Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg 
            340                 345                 350         


Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met 
        355                 360                 365             


Gly Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu 
    370                 375                 380                 


Ala Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys 
385                 390                 395                 400 


Ser Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly 
                405                 410                 415     


Asp Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly 
            420                 425                 430         


Arg Leu Val Pro Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr 
        435                 440                 445             


Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln 
    450                 455                 460                 


Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
465                 470                 475         


<210>  61
<211>  333
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TREM2-2A-DAP12trunc_2A_GFP

<400>  61

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu Gly Arg Gly 
225                 230                 235                 240 


Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Met Gly 
                245                 250                 255     


Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu Leu Leu Ala 
            260                 265                 270         


Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser Asp Cys Ser 
        275                 280                 285             


Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val Met Gly Asp 
    290                 295                 300                 


Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe Leu Gly Arg 
305                 310                 315                 320 


Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr 
                325                 330             


<210>  62
<211>  1380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREM2-CD3z-2A-DAP10_2A-GFP

<400>  62
atggagcctc tccggctgct catcttactc tttgtcacag agctgtccgg agcccacaac       60

accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc ctatgactcc      120

atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa gggcccatgc      180

cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag gtggaatggg      240

agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct gcggaatcta      300

caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga ggctgacacc      360

ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga tgctggagat      420

ctctggttcc ccggggagtc tgagagcttc gaggatgccc atagcatctc caggagcctc      480

ttggaaggag aaatcccctt cccacccact tccatccttc tcctcctggc ctgcatcttt      540

ctcatcaaga ttctagcagc cagcgccctc tgggctgcag cctggcatgg acagaagcca      600

gggacacatc cacccagtga actggactgt ggccatgacc cagggtatca gctccaaact      660

ctgccagggc tgagagacac gagagtgaag ttcagcagga gcgcagacgc ccccgcgtac      720

cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga ggagtacgat      780

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgca gagaaggaag      840

aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt      900

gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt      960

ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc     1020

gcccgcgcca aaaggtctgg ctccggtgag ggcagaggaa gtcttataac atgcggtgac     1080

gtggaggaga atcccggccc tatgatccat ctgggtcaca tcctcttcct gcttttgctc     1140

ccagtggctg cagctcagac gactccagga gagagatcat cactccctgc cttttaccct     1200

ggcacttcag gctcttgttc cggatgtggg tccctctctc tgccgctcct ggcaggcctc     1260

gtggctgctg atgcggtggc atcgctgctc atcgtggggg cggtgttcct gtgcgcacgc     1320

ccacgccgca gccccgccca agaagatggc aaagtctaca tcaacatgcc aggcaggggc     1380


<210>  63
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREM2-CD3z-2A-DAP10-CD28_2A_GFP

<400>  63
atggagcctc tccggctgct catcttactc tttgtcacag agctgtccgg agcccacaac       60

accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc ctatgactcc      120

atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa gggcccatgc      180

cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag gtggaatggg      240

agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct gcggaatcta      300

caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga ggctgacacc      360

ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga tgctggagat      420

ctctggttcc ccggggagtc tgagagcttc gaggatgccc atagcatctc caggagcctc      480

ttggaaggag aaatcccctt cccacccact tccatccttc tcctcctggc ctgcatcttt      540

ctcatcaaga ttctagcagc cagcgccctc tgggctgcag cctggcatgg acagaagcca      600

gggacacatc cacccagtga actggactgt ggccatgacc cagggtatca gctccaaact      660

ctgccagggc tgagagacac gagagtgaag ttcagcagga gcgcagacgc ccccgcgtac      720

cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga ggagtacgat      780

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgca gagaaggaag      840

aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt      900

gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt      960

ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc     1020

gcccgcgcca aaaggtctgg ctccggtgag ggcagaggaa gtcttataac atgcggtgac     1080

gtggaggaga atcccggccc tatgatccat ctgggtcaca tcctcttcct gcttttgctc     1140

ccagtggctg cagctcagac gactccagga gagagatcat cactccctgc cttttaccct     1200

ggcacttcag gctcttgttc cggatgtggg tccctctctc tgccgctcct ggcaggcctc     1260

gtggctgctg atgcggtggc atcgctgctc atcgtggggg cggtgttcct gtgcgcacgc     1320

ccacgccgca gccccgccca agaagatggc aaagtctaca tcaacatgcc aggcaggggc     1380

aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc     1440

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc     1500

tcc                                                                   1503


<210>  64
<211>  1224
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREM2-CD28-2A-DAP12_2A_GFP

<400>  64
atggagcctc tccggctgct catcttactc tttgtcacag agctgtccgg agcccacaac       60

accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc ctatgactcc      120

atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa gggcccatgc      180

cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag gtggaatggg      240

agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct gcggaatcta      300

caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga ggctgacacc      360

ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga tgctggagat      420

ctctggttcc ccggggagtc tgagagcttc gaggatgccc atagcatctc caggagcctc      480

ttggaaggag aaatcccctt cccacccact tccatccttc tcctcctggc ctgcatcttt      540

ctcatcaaga ttctagcagc cagcgccctc tgggctgcag cctggcatgg acagaagcca      600

gggacacatc cacccagtga actggactgt ggccatgacc cagggtatca gctccaaact      660

ctgccagggc tgagagacac gaggagtaag aggagcaggc tcctgcacag tgactacatg      720

aacatgactc cccgccgccc cgggcccacc cgcaagcatt accagcccta tgccccacca      780

cgcgacttcg cagcctatcg ctccgcccgc gccaaaaggt ctggctccgg tgagggcaga      840

ggaagtctta taacatgcgg tgacgtggag gagaatcccg gccctatggg gggacttgaa      900

ccctgcagca ggctcctgct cctgcctctc ctgctggctg taagtggtct ccgtcctgtc      960

caggcccagg cccagagcga ttgcagttgc tctacggtga gcccgggcgt gctggcaggg     1020

atcgtgatgg gagacctggt gctgacagtg ctcattgccc tggccgtgta cttcctgggc     1080

cggctggtcc ctcgggggcg aggggctgcg gaggcagcga cccggaaaca gcgtatcact     1140

gagaccgagt cgccttatca ggagctccag ggtcagaggt cggatgtcta cagcgacctc     1200

aacacacaga ggccgtatta caaa                                            1224


<210>  65
<211>  1431
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREM2-CD3z-2A-DAP12trunc-CD28_2A_GFP

<400>  65
atggagcctc tccggctgct catcttactc tttgtcacag agctgtccgg agcccacaac       60

accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc ctatgactcc      120

atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa gggcccatgc      180

cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag gtggaatggg      240

agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct gcggaatcta      300

caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga ggctgacacc      360

ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga tgctggagat      420

ctctggttcc ccggggagtc tgagagcttc gaggatgccc atagcatctc caggagcctc      480

ttggaaggag aaatcccctt cccacccact tccatccttc tcctcctggc ctgcatcttt      540

ctcatcaaga ttctagcagc cagcgccctc tgggctgcag cctggcatgg acagaagcca      600

gggacacatc cacccagtga actggactgt ggccatgacc cagggtatca gctccaaact      660

ctgccagggc tgagagacac gagagtgaag ttcagcagga gcgcagacgc ccccgcgtac      720

cagcagggcc agaaccagct ctataacgag ctcaatctag gacgaagaga ggagtacgat      780

gttttggaca agagacgtgg ccgggaccct gagatggggg gaaagccgca gagaaggaag      840

aaccctcagg aaggcctgta caatgaactg cagaaagata agatggcgga ggcctacagt      900

gagattggga tgaaaggcga gcgccggagg ggcaaggggc acgatggcct ttaccagggt      960

ctcagtacag ccaccaagga cacctacgac gcccttcaca tgcaggccct gccccctcgc     1020

gcccgcgcca aaaggtctgg ctccggtgag ggcagaggaa gtcttataac atgcggtgac     1080

gtggaggaga atcccggccc tatgggggga cttgaaccct gcagcaggct cctgctcctg     1140

cctctcctgc tggctgtaag tggtctccgt cctgtccagg cccaggccca gagcgattgc     1200

agttgctcta cggtgagccc gggcgtgctg gcagggatcg tgatgggaga cctggtgctg     1260

acagtgctca ttgccctggc cgtgtacttc ctgggccggc tggtccctag gagtaagagg     1320

agcaggctcc tgcacagtga ctacatgaac atgactcccc gccgccccgg gcccacccgc     1380

aagcattacc agccctatgc cccaccacgc gacttcgcag cctatcgctc c              1431


<210>  66
<211>  999
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TREM2-2A-DAP12trunc_2A_GFP

<400>  66
atggagcctc tccggctgct catcttactc tttgtcacag agctgtccgg agcccacaac       60

accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc ctatgactcc      120

atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa gggcccatgc      180

cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag gtggaatggg      240

agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct gcggaatcta      300

caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga ggctgacacc      360

ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga tgctggagat      420

ctctggttcc ccggggagtc tgagagcttc gaggatgccc atagcatctc caggagcctc      480

ttggaaggag aaatcccctt cccacccact tccatccttc tcctcctggc ctgcatcttt      540

ctcatcaaga ttctagcagc cagcgccctc tgggctgcag cctggcatgg acagaagcca      600

gggacacatc cacccagtga actggactgt ggccatgacc cagggtatca gctccaaact      660

ctgccagggc tgagagacac ggcccgcgcc aaaaggtctg gctccggtga gggcagagga      720

agtcttataa catgcggtga cgtggaggag aatcccggcc ctatgggggg acttgaaccc      780

tgcagcaggc tcctgctcct gcctctcctg ctggctgtaa gtggtctccg tcctgtccag      840

gcccaggccc agagcgattg cagttgctct acggtgagcc cgggcgtgct ggcagggatc      900

gtgatgggag acctggtgct gacagtgctc attgccctgg ccgtgtactt cctgggccgg      960

ctggtccctc gggggcgagg ggctgcggag gcagcgacc                             999


<210>  67
<211>  153
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant TREM2 ligand binding domain and stalk domain

<400>  67

His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu Gln Val 
1               5                   10                  15      


Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys Ala Trp 
            20                  25                  30          


Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val Ser Thr 
        35                  40                  45              


His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly Ser Thr 
    50                  55                  60                  


Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr Leu Arg 
65                  70                  75                  80  


Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser Leu His 
                85                  90                  95      


Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val Leu Ala 
            100                 105                 110         


Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro Gly Glu 
        115                 120                 125             


Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu Leu Glu 
    130                 135                 140                 


Gly Glu Ile Pro Phe Pro Pro Thr Ser 
145                 150             


<210>  68
<211>  227
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Mutant TREM2 exodomain

<400>  68

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Ser Ile Ser Arg Ser Leu 
145                 150                 155                 160 


Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu Leu Leu Leu 
                165                 170                 175     


Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala Leu Trp Ala 
            180                 185                 190         


Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro Ser Glu Leu 
        195                 200                 205             


Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu Pro Gly Leu 
    210                 215                 220                 


Arg Asp Thr 
225         


<210>  69
<211>  370
<212>  PRT
<213>  Artificial sequence

<220>
<223>  WT-TREM2-2A-DAP12-2A-GFP

<400>  69

Met Glu Pro Leu Arg Leu Leu Ile Leu Leu Phe Val Thr Glu Leu Ser 
1               5                   10                  15      


Gly Ala His Asn Thr Thr Val Phe Gln Gly Val Ala Gly Gln Ser Leu 
            20                  25                  30          


Gln Val Ser Cys Pro Tyr Asp Ser Met Lys His Trp Gly Arg Arg Lys 
        35                  40                  45              


Ala Trp Cys Arg Gln Leu Gly Glu Lys Gly Pro Cys Gln Arg Val Val 
    50                  55                  60                  


Ser Thr His Asn Leu Trp Leu Leu Ser Phe Leu Arg Arg Trp Asn Gly 
65                  70                  75                  80  


Ser Thr Ala Ile Thr Asp Asp Thr Leu Gly Gly Thr Leu Thr Ile Thr 
                85                  90                  95      


Leu Arg Asn Leu Gln Pro His Asp Ala Gly Leu Tyr Gln Cys Gln Ser 
            100                 105                 110         


Leu His Gly Ser Glu Ala Asp Thr Leu Arg Lys Val Leu Val Glu Val 
        115                 120                 125             


Leu Ala Asp Pro Leu Asp His Arg Asp Ala Gly Asp Leu Trp Phe Pro 
    130                 135                 140                 


Gly Glu Ser Glu Ser Phe Glu Asp Ala His Val Glu His Ser Ile Ser 
145                 150                 155                 160 


Arg Ser Leu Leu Glu Gly Glu Ile Pro Phe Pro Pro Thr Ser Ile Leu 
                165                 170                 175     


Leu Leu Leu Ala Cys Ile Phe Leu Ile Lys Ile Leu Ala Ala Ser Ala 
            180                 185                 190         


Leu Trp Ala Ala Ala Trp His Gly Gln Lys Pro Gly Thr His Pro Pro 
        195                 200                 205             


Ser Glu Leu Asp Cys Gly His Asp Pro Gly Tyr Gln Leu Gln Thr Leu 
    210                 215                 220                 


Pro Gly Leu Arg Asp Thr Ala Arg Ala Lys Arg Ser Gly Ser Gly Glu 
225                 230                 235                 240 


Gly Arg Gly Ser Leu Ile Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
                245                 250                 255     


Pro Met Gly Gly Leu Glu Pro Cys Ser Arg Leu Leu Leu Leu Pro Leu 
            260                 265                 270         


Leu Leu Ala Val Ser Gly Leu Arg Pro Val Gln Ala Gln Ala Gln Ser 
        275                 280                 285             


Asp Cys Ser Cys Ser Thr Val Ser Pro Gly Val Leu Ala Gly Ile Val 
    290                 295                 300                 


Met Gly Asp Leu Val Leu Thr Val Leu Ile Ala Leu Ala Val Tyr Phe 
305                 310                 315                 320 


Leu Gly Arg Leu Val Pro Arg Gly Arg Gly Ala Ala Glu Ala Ala Thr 
                325                 330                 335     


Arg Lys Gln Arg Ile Thr Glu Thr Glu Ser Pro Tyr Gln Glu Leu Gln 
            340                 345                 350         


Gly Gln Arg Ser Asp Val Tyr Ser Asp Leu Asn Thr Gln Arg Pro Tyr 
        355                 360                 365             


Tyr Lys 
    370 


<210>  70
<211>  1110
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WT-TREM2-2A-DAP12-2A-GFP

<400>  70
atggagcctc tccggctgct catcttactc tttgtcacag agctgtccgg agcccacaac       60

accacagtgt tccagggcgt ggcgggccag tccctgcagg tgtcttgccc ctatgactcc      120

atgaagcact gggggaggcg caaggcctgg tgccgccagc tgggagagaa gggcccatgc      180

cagcgtgtgg tcagcacgca caacttgtgg ctgctgtcct tcctgaggag gtggaatggg      240

agcacagcca tcacagacga taccctgggt ggcactctca ccattacgct gcggaatcta      300

caaccccatg atgcgggtct ctaccagtgc cagagcctcc atggcagtga ggctgacacc      360

ctcaggaagg tcctggtgga ggtgctggca gaccccctgg atcaccggga tgctggagat      420

ctctggttcc ccggggagtc tgagagcttc gaggatgccc atgtggagca cagcatctcc      480

aggagcctct tggaaggaga aatccccttc ccacccactt ccatccttct cctcctggcc      540

tgcatctttc tcatcaagat tctagcagcc agcgccctct gggctgcagc ctggcatgga      600

cagaagccag ggacacatcc acccagtgaa ctggactgtg gccatgaccc agggtatcag      660

ctccaaactc tgccagggct gagagacacg gcccgcgcca aaaggtctgg ctccggtgag      720

ggcagaggaa gtcttataac atgcggtgac gtggaggaga atcccggccc tatgggggga      780

cttgaaccct gcagcaggct cctgctcctg cctctcctgc tggctgtaag tggtctccgt      840

cctgtccagg cccaggccca gagcgattgc agttgctcta cggtgagccc gggcgtgctg      900

gcagggatcg tgatgggaga cctggtgctg acagtgctca ttgccctggc cgtgtacttc      960

ctgggccggc tggtccctcg ggggcgaggg gctgcggagg cagcgacccg gaaacagcgt     1020

atcactgaga ccgagtcgcc ttatcaggag ctccagggtc agaggtcgga tgtctacagc     1080

gacctcaaca cacagaggcc gtattacaaa                                      1110


