               SEQUENCE LISTING

<110> Bavarian Nordic A/S

<120> A RECOMBINANT MODIFIED VACCINIA VIRUS ANKARA (MVA) VACCINE AGAINST CORONAVIRUS DISEASE

<130> BN102PCT

<150> EP20179347.8
<151> 2020-06-10

<160> 27

<170> BiSSAP 1.3.6

<210> 1
<211> 1273
<212> PRT
<213> SARS coronavirus


<220> 
<223> SARS-CoV-2 S protein, full-length

<400> 1
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     
Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
        995                 1000                1005            
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
    1010                1015                1020                
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
1025                1030                1035                1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
                1045                1050                1055    
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
            1060                1065                1070        
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
        1075                1080                1085            
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
    1090                1095                1100                
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
1105                1110                1115                1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
                1125                1130                1135    
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
            1140                1145                1150        
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
        1155                1160                1165            
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
    1170                1175                1180                
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
1185                1190                1195                1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 
                1205                1210                1215    
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 
            1220                1225                1230        
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 
        1235                1240                1245            
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 
    1250                1255                1260                
Leu Lys Gly Val Lys Leu His Tyr Thr 
1265                1270            

<210> 2
<211> 3822
<212> DNA
<213> SARS coronavirus


<220> 
<223> SARS-CoV-2 S protein, full-length

<400> 2
atgtttgttt ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc       60

agaactcaat taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac      120

aaagttttca gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc      180

aatgttactt ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat      240

aaccctgtcc taccatttaa tgatggtgtt tattttgctt ccactgagaa gtctaacata      300

ataagaggct ggatttttgg tactacttta gattcgaaga cccagtccct acttattgtt      360

aataacgcta ctaatgttgt tattaaagtc tgtgaatttc aattttgtaa tgatccattt      420

ttgggtgttt attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat      480

tctagtgcga ataattgcac ttttgaatat gtctctcagc cttttcttat ggaccttgaa      540

ggaaaacagg gtaatttcaa aaatcttagg gaatttgtgt ttaagaatat tgatggttat      600

tttaaaatat attctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt      660

tcggctttag aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact      720

ttacttgctt tacatagaag ttatttgact cctggtgatt cttcttcagg ttggacagct      780

ggtgctgcag cttattatgt gggttatctt caacctagga cttttctatt aaaatataat      840

gaaaatggaa ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag      900

tgtacgttga aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc      960

caaccaacag aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa     1020

gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac     1080

tgtgttgctg attattctgt cctatataat tccgcatcat tttccacttt taagtgttat     1140

ggagtgtctc ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt     1200

gtaattagag gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat     1260

tataattata aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat     1320

cttgattcta aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat     1380

ctcaaacctt ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt     1440

aatggtgttg aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact     1500

aatggtgttg gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca     1560

ccagcaactg tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat     1620

ttcaacttca atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg     1680

cctttccaac aatttggcag agacattgct gacactactg atgctgtccg tgatccacag     1740

acacttgaga ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca     1800

ggaacaaata cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc     1860

cctgttgcta ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct     1920

aatgtttttc aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat     1980

gagtgtgaca tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct     2040

cctcggcggg cacgtagtgt agctagtcaa tccatcattg cctacactat gtcacttggt     2100

gcagaaaatt cagttgctta ctctaataac tctattgcca tacccacaaa ttttactatt     2160

agtgttacca cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg     2220

tacatttgtg gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt     2280

acacaattaa accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa     2340

gtttttgcac aagtcaaaca aatttacaaa acaccaccaa ttaaagattt tggtggtttt     2400

aatttttcac aaatattacc agatccatca aaaccaagca agaggtcatt tattgaagat     2460

ctacttttca acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc     2520

cttggtgata ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt     2580

ttgccacctt tgctcacaga tgaaatgatt gctcaataca cttctgcact gttagcgggt     2640

acaatcactt ctggttggac ctttggtgca ggtgctgcat tacaaatacc atttgctatg     2700

caaatggctt ataggtttaa tggtattgga gttacacaga atgttctcta tgagaaccaa     2760

aaattgattg ccaaccaatt taatagtgct attggcaaaa ttcaagactc actttcttcc     2820

acagcaagtg cacttggaaa acttcaagat gtggtcaacc aaaatgcaca agctttaaac     2880

acgcttgtta aacaacttag ctccaatttt ggtgcaattt caagtgtttt aaatgatatc     2940

ctttcacgtc ttgacaaagt tgaggctgaa gtgcaaattg ataggttgat cacaggcaga     3000

cttcaaagtt tgcagacata tgtgactcaa caattaatta gagctgcaga aatcagagct     3060

tctgctaatc ttgctgctac taaaatgtca gagtgtgtac ttggacaatc aaaaagagtt     3120

gatttttgtg gaaagggcta tcatcttatg tccttccctc agtcagcacc tcatggtgta     3180

gtcttcttgc atgtgactta tgtccctgca caagaaaaga acttcacaac tgctcctgcc     3240

atttgtcatg atggaaaagc acactttcct cgtgaaggtg tctttgtttc aaatggcaca     3300

cactggtttg taacacaaag gaatttttat gaaccacaaa tcattactac agacaacaca     3360

tttgtgtctg gtaactgtga tgttgtaata ggaattgtca acaacacagt ttatgatcct     3420

ttgcaacctg aattagactc attcaaggag gagttagata aatattttaa gaatcataca     3480

tcaccagatg ttgatttagg tgacatctct ggcattaatg cttcagttgt aaacattcaa     3540

aaagaaattg accgcctcaa tgaggttgcc aagaatttaa atgaatctct catcgatctc     3600

caagaacttg gaaagtatga gcagtatata aaatggccat ggtacatttg gctaggtttt     3660

atagctggct tgattgccat agtaatggtg acaattatgc tttgctgtat gaccagttgc     3720

tgtagttgtc tcaagggctg ttgttcttgt ggatcctgct gcaaatttga tgaagacgac     3780

tctgagccag tgctcaaagg agtcaaatta cattacacat aa                        3822


<210> 3
<211> 194
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S RBD, modified

<400> 3
Ala Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      
Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          
Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              
Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  
Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  
Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      
Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         
Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             
Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 
Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 
Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     
Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         
Thr Val 
        

<210> 4
<211> 582
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S RBD, modified

<400> 4
gccatcacca atctgtgccc ttttggcgag gtgttcaacg ccaccagatt cgcctctgtg      60

tacgcctgga accggaagcg gatcagcaat tgcgttgccg actacagcgt gctgtacaac     120

tctgccagct tctccacctt caagtgctat ggcgtgtctc ctaccaagct gaacgacctg     180

tgcttcacca acgtgtacgc tgacagcttc gtgatcagag gcgacgaagt gagacagatt     240

gctcctggac agacaggcaa gattgccgat tacaactaca agctccctga cgacttcaca     300

ggctgtgtga ttgcctggaa cagcaacaac ctggacagca aagtcggagg taactacaac     360

tacctgtaca ggctgtttcg gaagtccaac ctgaagcctt tcgagagaga catcagcacc     420

gagatctatc aggcaggcag cacaccttgc aatggcgtgg aaggcttcaa ctgctacttc     480

ccactgcagt cctacggctt ccagcctaca aatggagtgg gctaccagcc ttacagagtg     540

gtggtgctga gcttcgagct gctgcatgct cctgccacag tg                        582


<210> 5
<211> 231
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein S1 domain, partial, modified

<400> 5
Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Ala Ile Thr Asn 
1               5                   10                  15      
Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          
Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              
Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  
Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  
Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      
Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         
Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             
Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 
Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 
Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     
Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         
Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             
Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn 
    210                 215                 220                 
Phe Asn Gly Leu Thr Gly Thr 
225                 230     

<210> 6
<211> 693
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein S1 domain, partial, modified

<400> 6
agagtgcagc ccacagagtc tatcgtgcgg ttccctgcca tcaccaatct gtgccctttt      60

ggcgaggtgt tcaacgccac cagattcgcc tctgtgtacg cctggaaccg gaagcggatc     120

agcaattgcg ttgccgacta cagcgtgctg tacaactctg ccagcttctc caccttcaag     180

tgctatggcg tgtctcctac caagctgaac gacctgtgct tcaccaacgt gtacgctgac     240

agcttcgtga tcagaggcga cgaagtgaga cagattgctc ctggacagac aggcaagatt     300

gccgattaca actacaagct ccctgacgac ttcacaggct gtgtgattgc ctggaacagc     360

aacaacctgg acagcaaagt cggaggtaac tacaactacc tgtacaggct gtttcggaag     420

tccaacctga agcctttcga gagagacatc agcaccgaga tctatcaggc aggcagcaca     480

ccttgcaatg gcgtggaagg cttcaactgc tacttcccac tgcagtccta cggcttccag     540

cctacaaatg gagtgggcta ccagccttac agagtggtgg tgctgagctt cgagctgctg     600

catgctcctg ccacagtgtg cggacctaag aaaagcacca acctggtgaa gaacaaatgc     660

gtgaacttca acttcaatgg cctgacaggc acc                                  693


<210> 7
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human IgGH signal peptide

<400> 7
Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      
Val Gln Cys 
            

<210> 8
<211> 57
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human IgGH signal peptide

<400> 8
atggaattcg gactgagctg ggtgttcctg gtcgccattc tgaaaggcgt gcagtgc        57


<210> 9
<211> 250
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein S1 domain, partial, modified plus human IgGH
      signal peptide ("SARS-2 S-RBD-1")

<400> 9
Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      
Val Gln Cys Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Ala 
            20                  25                  30          
Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe 
        35                  40                  45              
Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala 
    50                  55                  60                  
Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys 
65                  70                  75                  80  
Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val 
                85                  90                  95      
Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala 
            100                 105                 110         
Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp 
        115                 120                 125             
Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser 
    130                 135                 140                 
Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser 
145                 150                 155                 160 
Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala 
                165                 170                 175     
Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro 
            180                 185                 190         
Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro 
        195                 200                 205             
Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr 
    210                 215                 220                 
Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val 
225                 230                 235                 240 
Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
                245                 250 

<210> 10
<211> 756
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein S1 domain, partial, modified plus human IgGH
      signal peptide ("SARS-2 S-RBD-1")

<400> 10
atggaattcg gactgagctg ggtgttcctg gtcgccattc tgaaaggcgt gcagtgcaga      60

gtgcagccca cagagtctat cgtgcggttc cctgccatca ccaatctgtg cccttttggc     120

gaggtgttca acgccaccag attcgcctct gtgtacgcct ggaaccggaa gcggatcagc     180

aattgcgttg ccgactacag cgtgctgtac aactctgcca gcttctccac cttcaagtgc     240

tatggcgtgt ctcctaccaa gctgaacgac ctgtgcttca ccaacgtgta cgctgacagc     300

ttcgtgatca gaggcgacga agtgagacag attgctcctg gacagacagg caagattgcc     360

gattacaact acaagctccc tgacgacttc acaggctgtg tgattgcctg gaacagcaac     420

aacctggaca gcaaagtcgg aggtaactac aactacctgt acaggctgtt tcggaagtcc     480

aacctgaagc ctttcgagag agacatcagc accgagatct atcaggcagg cagcacacct     540

tgcaatggcg tggaaggctt caactgctac ttcccactgc agtcctacgg cttccagcct     600

acaaatggag tgggctacca gccttacaga gtggtggtgc tgagcttcga gctgctgcat     660

gctcctgcca cagtgtgcgg acctaagaaa agcaccaacc tggtgaagaa caaatgcgtg     720

aacttcaact tcaatggcct gacaggcacc tgataa                               756


<210> 11
<211> 275
<212> PRT
<213> SARS coronavirus


<220> 
<223> SARS-CoV-2 protein 3a, full-length

<400> 11
Met Asp Leu Phe Met Arg Ile Phe Thr Ile Gly Thr Val Thr Leu Lys 
1               5                   10                  15      
Gln Gly Glu Ile Lys Asp Ala Thr Pro Ser Asp Phe Val Arg Ala Thr 
            20                  25                  30          
Ala Thr Ile Pro Ile Gln Ala Ser Leu Pro Phe Gly Trp Leu Ile Val 
        35                  40                  45              
Gly Val Ala Leu Leu Ala Val Phe Gln Ser Ala Ser Lys Ile Ile Thr 
    50                  55                  60                  
Leu Lys Lys Arg Trp Gln Leu Ala Leu Ser Lys Gly Val His Phe Val 
65                  70                  75                  80  
Cys Asn Leu Leu Leu Leu Phe Val Thr Val Tyr Ser His Leu Leu Leu 
                85                  90                  95      
Val Ala Ala Gly Leu Glu Ala Pro Phe Leu Tyr Leu Tyr Ala Leu Val 
            100                 105                 110         
Tyr Phe Leu Gln Ser Ile Asn Phe Val Arg Ile Ile Met Arg Leu Trp 
        115                 120                 125             
Leu Cys Trp Lys Cys Arg Ser Lys Asn Pro Leu Leu Tyr Asp Ala Asn 
    130                 135                 140                 
Tyr Phe Leu Cys Trp His Thr Asn Cys Tyr Asp Tyr Cys Ile Pro Tyr 
145                 150                 155                 160 
Asn Ser Val Thr Ser Ser Ile Val Ile Thr Ser Gly Asp Gly Thr Thr 
                165                 170                 175     
Ser Pro Ile Ser Glu His Asp Tyr Gln Ile Gly Gly Tyr Thr Glu Lys 
            180                 185                 190         
Trp Glu Ser Gly Val Lys Asp Cys Val Val Leu His Ser Tyr Phe Thr 
        195                 200                 205             
Ser Asp Tyr Tyr Gln Leu Tyr Ser Thr Gln Leu Ser Thr Asp Thr Gly 
    210                 215                 220                 
Val Glu His Val Thr Phe Phe Ile Tyr Asn Lys Ile Val Asp Glu Pro 
225                 230                 235                 240 
Glu Glu His Val Gln Ile His Thr Ile Asp Gly Ser Ser Gly Val Val 
                245                 250                 255     
Asn Pro Val Met Glu Pro Ile Tyr Asp Glu Pro Thr Thr Thr Thr Ser 
            260                 265                 270         
Val Pro Leu 
        275 

<210> 12
<211> 75
<212> PRT
<213> SARS coronavirus


<220> 
<223> SARS-CoV-2 protein E, full-length

<400> 12
Met Tyr Ser Phe Val Ser Glu Glu Thr Gly Thr Leu Ile Val Asn Ser 
1               5                   10                  15      
Val Leu Leu Phe Leu Ala Phe Val Val Phe Leu Leu Val Thr Leu Ala 
            20                  25                  30          
Ile Leu Thr Ala Leu Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn 
        35                  40                  45              
Val Ser Leu Val Lys Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn 
    50                  55                  60                  
Leu Asn Ser Ser Arg Val Pro Asp Leu Leu Val 
65                  70                  75  

<210> 13
<211> 222
<212> PRT
<213> SARS coronavirus


<220> 
<223> SARS-CoV-2 protein M, full-length

<400> 13
Met Ala Asp Ser Asn Gly Thr Ile Thr Val Glu Glu Leu Lys Lys Leu 
1               5                   10                  15      
Leu Glu Gln Trp Asn Leu Val Ile Gly Phe Leu Phe Leu Thr Trp Ile 
            20                  25                  30          
Cys Leu Leu Gln Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile 
        35                  40                  45              
Ile Lys Leu Ile Phe Leu Trp Leu Leu Trp Pro Val Thr Leu Ala Cys 
    50                  55                  60                  
Phe Val Leu Ala Ala Val Tyr Arg Ile Asn Trp Ile Thr Gly Gly Ile 
65                  70                  75                  80  
Ala Ile Ala Met Ala Cys Leu Val Gly Leu Met Trp Leu Ser Tyr Phe 
                85                  90                  95      
Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met Trp Ser Phe 
            100                 105                 110         
Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His Gly Thr Ile 
        115                 120                 125             
Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly Ala Val Ile 
    130                 135                 140                 
Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly Arg Cys Asp 
145                 150                 155                 160 
Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser Arg Thr Leu 
                165                 170                 175     
Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly Asp Ser Gly 
            180                 185                 190         
Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys Leu Asn Thr 
        195                 200                 205             
Asp His Ser Ser Ser Ser Asp Asn Ile Ala Leu Leu Val Gln 
    210                 215                 220         

<210> 14
<211> 28
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 protein 3a-1 fragment

<400> 14
Phe Gln Ser Ala Ser Lys Ile Ile Thr Leu Lys Lys Arg Trp Gln Leu 
1               5                   10                  15      
Ala Leu Ser Lys Gly Val His Phe Val Cys Asn Leu 
            20                  25              

<210> 15
<211> 98
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS CoV-2 protein 3a-2 fragment

<400> 15
Pro Ile Ser Glu His Asp Tyr Gln Ile Gly Gly Tyr Thr Glu Lys Trp 
1               5                   10                  15      
Glu Ser Gly Val Lys Asp Cys Val Val Leu His Ser Tyr Phe Thr Ser 
            20                  25                  30          
Asp Tyr Tyr Gln Leu Tyr Ser Thr Gln Leu Ser Thr Asp Thr Gly Val 
        35                  40                  45              
Glu His Val Thr Phe Phe Ile Tyr Asn Lys Ile Val Asp Glu Pro Glu 
    50                  55                  60                  
Glu His Val Gln Ile His Thr Ile Asp Gly Ser Ser Gly Val Val Asn 
65                  70                  75                  80  
Pro Val Met Glu Pro Ile Tyr Asp Glu Pro Thr Thr Thr Thr Ser Val 
                85                  90                  95      
Pro Leu 
        

<210> 16
<211> 36
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 protein E fragment

<400> 16
Arg Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn Val Ser Leu Val Lys 
1               5                   10                  15      
Pro Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn Leu Asn Ser Ser Arg 
            20                  25                  30          
Val Pro Asp Leu 
        35      

<210> 17
<211> 15
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 protein M-1 fragment

<400> 17
Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile Ile Lys Leu 
1               5                   10                  15  

<210> 18
<211> 119
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 protein M-2 fragment

<400> 18
Ser Tyr Phe Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg Ser Met 
1               5                   10                  15      
Trp Ser Phe Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro Leu His 
            20                  25                  30          
Gly Thr Ile Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val Ile Gly 
        35                  40                  45              
Ala Val Ile Leu Arg Gly His Leu Arg Ile Ala Gly His His Leu Gly 
    50                  55                  60                  
Arg Cys Asp Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala Thr Ser 
65                  70                  75                  80  
Arg Thr Leu Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val Ala Gly 
                85                  90                  95      
Asp Ser Gly Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn Tyr Lys 
            100                 105                 110         
Leu Asn Thr Asp His Ser Ser 
        115                 

<210> 19
<211> 297
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 protein 3a-1, 3a-2, E, M-1, M-2 fragments, fused

<400> 19
Met Phe Gln Ser Ala Ser Lys Ile Ile Thr Leu Lys Lys Arg Trp Gln 
1               5                   10                  15      
Leu Ala Leu Ser Lys Gly Val His Phe Val Cys Asn Leu Pro Ile Ser 
            20                  25                  30          
Glu His Asp Tyr Gln Ile Gly Gly Tyr Thr Glu Lys Trp Glu Ser Gly 
        35                  40                  45              
Val Lys Asp Cys Val Val Leu His Ser Tyr Phe Thr Ser Asp Tyr Tyr 
    50                  55                  60                  
Gln Leu Tyr Ser Thr Gln Leu Ser Thr Asp Thr Gly Val Glu His Val 
65                  70                  75                  80  
Thr Phe Phe Ile Tyr Asn Lys Ile Val Asp Glu Pro Glu Glu His Val 
                85                  90                  95      
Gln Ile His Thr Ile Asp Gly Ser Ser Gly Val Val Asn Pro Val Met 
            100                 105                 110         
Glu Pro Ile Tyr Asp Glu Pro Thr Thr Thr Thr Ser Val Pro Leu Arg 
        115                 120                 125             
Leu Cys Ala Tyr Cys Cys Asn Ile Val Asn Val Ser Leu Val Lys Pro 
    130                 135                 140                 
Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn Leu Asn Ser Ser Arg Val 
145                 150                 155                 160 
Pro Asp Leu Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile Ile 
                165                 170                 175     
Lys Leu Ser Tyr Phe Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg 
            180                 185                 190         
Ser Met Trp Ser Phe Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro 
        195                 200                 205             
Leu His Gly Thr Ile Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val 
    210                 215                 220                 
Ile Gly Ala Val Ile Leu Arg Gly His Leu Arg Ile Ala Gly His His 
225                 230                 235                 240 
Leu Gly Arg Cys Asp Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala 
                245                 250                 255     
Thr Ser Arg Thr Leu Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val 
            260                 265                 270         
Ala Gly Asp Ser Gly Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn 
        275                 280                 285             
Tyr Lys Leu Asn Thr Asp His Ser Ser 
    290                 295         

<210> 20
<211> 297
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 3aEM fusion protein, modified ("SARS-2 3aEM-1")

<400> 20
Met Phe Gln Ser Ala Ser Lys Ile Ile Thr Leu Lys Lys Arg Trp Gln 
1               5                   10                  15      
Leu Ala Leu Ser Lys Gly Val His Phe Val Cys Asn Leu Pro Ile Ser 
            20                  25                  30          
Glu His Asp Tyr Gln Ile Gly Gly Tyr Thr Glu Lys Trp Glu Ser Gly 
        35                  40                  45              
Val Lys Asp Cys Val Val Leu His Ser Tyr Phe Thr Ser Asp Tyr Tyr 
    50                  55                  60                  
Gln Leu Tyr Ser Thr Gln Leu Ser Thr Asp Thr Gly Val Glu His Val 
65                  70                  75                  80  
Thr Phe Phe Ile Tyr Asn Lys Ile Val Asp Glu Pro Glu Glu His Val 
                85                  90                  95      
Gln Ile His Thr Ile Asp Gly Ser Ser Gly Val Val Asn Pro Val Met 
            100                 105                 110         
Glu Pro Ile Tyr Asp Glu Pro Thr Thr Thr Thr Ser Val Pro Leu Arg 
        115                 120                 125             
Leu Cys Trp Phe Cys Cys Asn Ile Val Asn Val Ser Leu Val Lys Pro 
    130                 135                 140                 
Ser Phe Tyr Val Tyr Ser Arg Val Lys Asn Leu Asn Ser Ser Arg Val 
145                 150                 155                 160 
Pro Asp Leu Phe Ala Tyr Ala Asn Arg Asn Arg Phe Leu Tyr Ile Ile 
                165                 170                 175     
Lys Leu Asp Phe Phe Ile Ala Ser Phe Arg Leu Phe Ala Arg Thr Arg 
            180                 185                 190         
Ser Met Trp Ser Phe Asn Pro Glu Thr Asn Ile Leu Leu Asn Val Pro 
        195                 200                 205             
Leu His Gly Thr Ile Leu Thr Arg Pro Leu Leu Glu Ser Glu Leu Val 
    210                 215                 220                 
Ile Gly Ala Val Ile Leu Arg Gly His Leu Arg Ile Ala Gly His His 
225                 230                 235                 240 
Leu Gly Arg Cys Asp Ile Lys Asp Leu Pro Lys Glu Ile Thr Val Ala 
                245                 250                 255     
Thr Ser Arg Thr Leu Ser Tyr Tyr Lys Leu Gly Ala Ser Gln Arg Val 
            260                 265                 270         
Ala Gly Asp Ser Gly Phe Ala Ala Tyr Ser Arg Tyr Arg Ile Gly Asn 
        275                 280                 285             
Tyr Lys Leu Asn Thr Asp His Ser Ser 
    290                 295         

<210> 21
<211> 897
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 3aEM fusion protein, modified ("SARS-2 3aEM-1")

<400> 21
atgtttcagt ctgccagcaa gatcatcacc ctgaagaaga ggtggcagct ggctctgtct      60

aaaggtgtgc acttcgtgtg caacctgcct atcagcgagc acgactatca gatcggaggc     120

tacaccgaga agtgggagtc tggcgtgaag gactgtgtgg tgctgcacag ctacttcacc     180

agcgactact accagctgta ctctacccag ctgagcaccg acacaggcgt ggaacacgtg     240

accttcttca tctacaacaa gatcgtggac gaacccgaag aacacgtgca gatccacaca     300

atcgatggca gctctggcgt ggtcaaccca gtgatggaac ccatctacga cgagcctacc     360

accaccacaa gcgtgcctct gagactgtgc tggttctgct gcaacatcgt gaacgtgtcc     420

ctggtcaagc ccagcttcta cgtgtacagc agagtgaaga acctgaacag cagcagggtt     480

cccgacctgt tcgcctacgc caacagaaac agattcctgt acatcatcaa gctggatttc     540

ttcatcgcca gcttcagact gttcgcacgg accagatcca tgtggtcctt caatcccgag     600

acaaacatcc tgctgaatgt gcctctgcac ggcaccatcc tgacaagacc tctgctggaa     660

agcgagctgg ttatcggtgc cgtgatcctg agaggccacc tgagaattgc aggacaccac     720

ctgggcagat gcgacatcaa ggacctgcca aaagaaatca ccgtggccac cagcaggacc     780

ctgagctact acaagctggg agcctctcag agagtggctg gcgattctgg atttgcagcc     840

tacagcagat accggatcgg caactacaaa ctgaataccg accacagcag ctaatga        897


<210> 22
<211> 1261
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein, full-length, modified, minus signal peptide

<400> 22
Ser Gln Cys Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr 
1               5                   10                  15      
Thr Asn Ser Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg 
            20                  25                  30          
Ser Ser Val Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser 
        35                  40                  45              
Asn Val Thr Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr 
    50                  55                  60                  
Lys Arg Phe Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe 
65                  70                  75                  80  
Ala Ser Thr Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr 
                85                  90                  95      
Thr Leu Asp Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr 
            100                 105                 110         
Asn Val Val Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe 
        115                 120                 125             
Leu Gly Val Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu 
    130                 135                 140                 
Phe Arg Val Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser 
145                 150                 155                 160 
Gln Pro Phe Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn 
                165                 170                 175     
Leu Arg Glu Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr 
            180                 185                 190         
Ser Lys His Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe 
        195                 200                 205             
Ser Ala Leu Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr 
    210                 215                 220                 
Arg Phe Gln Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly 
225                 230                 235                 240 
Asp Ser Ser Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly 
                245                 250                 255     
Tyr Leu Gln Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr 
            260                 265                 270         
Ile Thr Asp Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys 
        275                 280                 285             
Cys Thr Leu Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser 
    290                 295                 300                 
Asn Phe Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile 
305                 310                 315                 320 
Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala 
                325                 330                 335     
Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp 
            340                 345                 350         
Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr 
        355                 360                 365             
Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr 
    370                 375                 380                 
Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro 
385                 390                 395                 400 
Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp 
                405                 410                 415     
Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys 
            420                 425                 430         
Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn 
        435                 440                 445             
Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly 
    450                 455                 460                 
Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu 
465                 470                 475                 480 
Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr 
                485                 490                 495     
Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val 
            500                 505                 510         
Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn 
        515                 520                 525             
Phe Asn Phe Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn 
    530                 535                 540                 
Lys Lys Phe Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr 
545                 550                 555                 560 
Thr Asp Ala Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr 
                565                 570                 575     
Pro Cys Ser Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr 
            580                 585                 590         
Ser Asn Gln Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val 
        595                 600                 605             
Pro Val Ala Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr 
    610                 615                 620                 
Ser Thr Gly Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly 
625                 630                 635                 640 
Ala Glu His Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala 
                645                 650                 655     
Gly Ile Cys Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala 
            660                 665                 670         
Ser Ser Val Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly 
        675                 680                 685             
Ala Glu Asn Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr 
    690                 695                 700                 
Asn Phe Thr Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr 
705                 710                 715                 720 
Lys Thr Ser Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu 
                725                 730                 735     
Cys Ser Asn Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn 
            740                 745                 750         
Arg Ala Leu Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu 
        755                 760                 765             
Val Phe Ala Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp 
    770                 775                 780                 
Phe Gly Gly Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro 
785                 790                 795                 800 
Ser Lys Arg Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu 
                805                 810                 815     
Ala Asp Ala Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile 
            820                 825                 830         
Ala Ala Arg Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val 
        835                 840                 845             
Leu Pro Pro Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala 
    850                 855                 860                 
Leu Leu Ala Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala 
865                 870                 875                 880 
Ala Leu Gln Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly 
                885                 890                 895     
Ile Gly Val Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala 
            900                 905                 910         
Asn Gln Phe Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser 
        915                 920                 925             
Thr Ala Ser Ala Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala 
    930                 935                 940                 
Gln Ala Leu Asn Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala 
945                 950                 955                 960 
Ile Ser Ser Val Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu 
                965                 970                 975     
Ala Glu Val Gln Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu 
            980                 985                 990         
Gln Thr Tyr Val Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala 
        995                 1000                1005            
Ser Ala Asn Leu Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln 
    1010                1015                1020                
Ser Lys Arg Val Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe 
1025                1030                1035                1040
Pro Gln Ser Ala Pro His Gly Val Val Phe Leu His Val Thr Tyr Val 
                1045                1050                1055    
Pro Ala Gln Glu Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp 
            1060                1065                1070        
Gly Lys Ala His Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr 
        1075                1080                1085            
His Trp Phe Val Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr 
    1090                1095                1100                
Thr Asp Asn Thr Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile 
1105                1110                1115                1120
Val Asn Asn Thr Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe 
                1125                1130                1135    
Lys Glu Glu Leu Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val 
            1140                1145                1150        
Asp Leu Gly Asp Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln 
        1155                1160                1165            
Lys Glu Ile Asp Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser 
    1170                1175                1180                
Leu Ile Asp Leu Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp 
1185                1190                1195                1200
Pro Trp Tyr Ile Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val 
                1205                1210                1215    
Met Val Thr Ile Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu 
            1220                1225                1230        
Lys Gly Cys Cys Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp 
        1235                1240                1245            
Ser Glu Pro Val Leu Lys Gly Val Lys Leu His Tyr Thr 
    1250                1255                1260    

<210> 23
<211> 3789
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein, full-length, modified, minus signal peptide

<400> 23
agccagtgtg tgaacctgac caccagaaca cagctgcctc cagcctacac caacagcttt       60

accagaggcg tgtactatcc cgacaaggtg ttcagatcca gcgtgctgca ctctacccag      120

gacctgttcc tgcctttctt cagcaacgtg acctggttcc acgccatcca cgtgagcggt      180

accaatggca ccaagagatt cgacaatccc gtgctgccct tcaacgacgg agtgtacttt      240

gccagcaccg agaagtccaa catcatcaga ggctggatct tcggcaccac actggacagc      300

aagacccaga gcctgctgat cgtgaacaac gccaccaacg tggtcatcaa agtgtgcgag      360

ttccagttct gcaacgatcc cttcctggga gtctactacc acaagaacaa caagagctgg      420

atggaaagcg agttcagagt gtacagctct gccaacaact gcaccttcga gtacgtgtcc      480

cagcctttcc tgatggacct ggaaggcaag cagggcaatt tcaagaatct ccgagagttc      540

gtgttcaaga acatcgacgg ctacttcaag atctacagca agcacacacc tatcaacctc      600

gtgagggatc tgcctcaggg cttctctgct ctggaacctc tggtggatct gcccatcggc      660

atcaacatca ctcggtttca gacactgctg gcactgcaca gaagctacct gacacctggc      720

gatagcagca gcggatggac agctggtgct gcagcttact atgtgggcta cctgcagcct      780

agaaccttcc tgctgaagta caacgagaac ggcaccatca ccgatgccgt ggattgtgct      840

ctggatcctc tgagcgagac aaagtgcacc ctgaagtcct tcaccgtgga gaagggcatc      900

taccagacca gcaacttcag ggtgcagccc accgaatcca tcgtgcggtt ccctaacatc      960

accaatctgt gtccctttgg cgaggtgttc aatgccacca gattcgcctc tgtgtacgcc     1020

tggaaccgga agcggatcag caattgcgtt gccgactact ccgtgctgta caactcagcc     1080

agcttcagca ccttcaagtg ctacggagtg agccctacca agctgaacga cctgtgcttc     1140

acaaacgtgt atgctgacag cttcgtgatc agaggagatg aagtgaggca gattgctcct     1200

ggacagacag gcaagattgc agattacaac tacaagctgc ctgacgactt cacaggctgt     1260

gtgattgcct ggaacagcaa caacctggac tccaaagtcg gaggcaacta caactacctg     1320

tacagactgt tcaggaagtc caatctgaag cccttcgaac gggacatctc caccgagatc     1380

tatcaggctg gcagcacacc ttgtaatgga gtggaaggct tcaactgcta cttcccactg     1440

cagtcctacg gctttcagcc cacaaatggc gtgggctatc agccctacag agtggtggtg     1500

ctgagcttcg aactgctgca tgctcctgcc acagtgtgcg gacctaagaa atccaccaat     1560

ctcgtgaaga acaaatgcgt gaacttcaac ttcaacggtc tgaccggtac aggcgtgctg     1620

acagagagca acaagaagtt cctgccattc cagcagtttg gcagggatat cgcagatacc     1680

acagacgctg ttagagatcc acagacactg gaaatcctgg acatcacacc ttgcagcttc     1740

ggaggcgtgt ctgtgatcac acctggcacc aacaccagca atcaggtggc agttctgtac     1800

caggacgtga actgtaccga agttcccgtg gccattcatg ccgatcagct gacacctaca     1860

tggagggtgt actccaccgg aagcaatgtg tttcagacca gagctggctg tctgatcgga     1920

gccgagcacg tgaacaatag ctacgagtgc gacatcccta tcggagctgg catctgtgcc     1980

agctaccaga cacagacaaa cagccctggc tctgcctctt ccgtggccag ccagtccatc     2040

attgcctaca caatgtctct gggagccgag aacagcgtgg cctactccaa caactctatc     2100

gctatcccta ccaacttcac aatcagcgtg accacagaga tcctgcctgt gtccatgacc     2160

aagaccagcg tggactgcac catgtacatc tgtggcgatt ccaccgagtg ctccaacctg     2220

ctgctgcagt acggcagctt ctgcacccag ctgaatagag ccctgacagg gatcgctgtg     2280

gaacaggaca agaacaccca agaggtgttc gcacaagtga agcagatcta caagacacct     2340

cctatcaagg acttcggagg attcaacttt agccagattc tgcctgatcc tagcaagcca     2400

agcaagcgga gcttcatcga ggacctgctg ttcaacaaag tgacacttgc cgacgctggc     2460

ttcatcaagc agtatggcga ttgtctggga gacattgctg ccagggatct gatttgtgcc     2520

cagaagttca acggactgac agtgctgcct cctctgctga ccgatgagat gatcgctcag     2580

tacacatctg ccctgctggc tggcacaatc acaagtggct ggacatttgg agctggagct     2640

gctctgcaga ttccctttgc tatgcagatg gcctaccggt tcaacggcat cggagtgacc     2700

cagaatgtgc tgtacgagaa ccagaagctg atcgccaacc agttcaactc tgccatcggc     2760

aagatccagg acagcctgag cagcacagca agcgcactgg gaaagctgca ggacgtggtc     2820

aaccagaatg cccaggcact gaacaccctg gtcaagcagc tgtcctccaa cttcggagcc     2880

atcagctctg tgctgaacga tatcctgagc agactggacc ctcctgaagc cgaggtgcag     2940

atcgacaggc tgatcacagg cagactgcag agcctccaga catacgtgac ccagcagctg     3000

atcagagctg ccgagattag agcctctgcc aatctggcag ccaccaagat gtctgagtgt     3060

gtgctgggac agagcaagag agtggacttc tgtggcaagg gctaccacct gatgagcttc     3120

cctcagtctg cacctcatgg cgtggtgttt ctgcacgtga catatgtgcc agctcaagag     3180

aagaacttca ccaccgctcc agccatctgc cacgacggta aagcccactt tcctagagaa     3240

ggcgtgttcg tgtccaacgg cacccattgg ttcgtgacac agcggaactt ctacgagcct     3300

cagatcatca ccaccgacaa caccttcgtg tctggcaact gcgacgtcgt gatcggcatt     3360

gtcaacaata ccgtgtacga ccctctgcag ccagagctcg acagcttcaa agaggaactg     3420

gacaagtact tcaagaacca cacaagccct gacgtggacc tgggagatat cagcggaatc     3480

aatgccagcg tcgtgaacat ccagaaagag attgacagac tgaacgaggt ggccaagaat     3540

ctgaacgaga gcctgatcga cctgcaagaa cttgggaagt acgagcagta catcaagtgg     3600

ccttggtaca tctggctggg cttcatcgca ggactgattg ccatcgtgat ggtcacaatc     3660

atgctgtgtt gcatgaccag ctgctgtagc tgtctgaagg gctgttgtag ctgtggcagc     3720

tgctgcaagt tcgacgagga cgattctgag ccagtgctga aaggcgtgaa actgcactac     3780

acatagtaa                                                             3789


<210> 24
<211> 1273
<212> PRT
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein, full-length, modified, plus signal peptide
      ("SARS-2 S-FS-1")

<400> 24
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      
Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          
Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              
His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  
Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  
Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      
Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         
Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             
Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 
Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 
Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     
Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         
Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             
Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 
Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 
Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     
Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         
Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             
Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 
Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 
Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     
Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         
Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             
Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 
Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 
Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     
Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         
Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             
Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 
Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 
Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     
Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         
Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             
Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 
Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 
Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     
Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         
Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             
Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 
His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 
Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     
Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         
Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser Ser Val Ala 
        675                 680                 685             
Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 
Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 
Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     
Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         
Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             
Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 
Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 
Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     
Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         
Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             
Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 
Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 
Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     
Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         
Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             
Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 
Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 
Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     
Leu Asn Asp Ile Leu Ser Arg Leu Asp Pro Pro Glu Ala Glu Val Gln 
            980                 985                 990         
Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val 
        995                 1000                1005            
Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu 
    1010                1015                1020                
Ala Ala Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val 
1025                1030                1035                1040
Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala 
                1045                1050                1055    
Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu 
            1060                1065                1070        
Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His 
        1075                1080                1085            
Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val 
    1090                1095                1100                
Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr 
1105                1110                1115                1120
Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr 
                1125                1130                1135    
Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu 
            1140                1145                1150        
Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp 
        1155                1160                1165            
Ile Ser Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp 
    1170                1175                1180                
Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu 
1185                1190                1195                1200
Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile 
                1205                1210                1215    
Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile 
            1220                1225                1230        
Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys 
        1235                1240                1245            
Ser Cys Gly Ser Cys Cys Lys Phe Asp Glu Asp Asp Ser Glu Pro Val 
    1250                1255                1260                
Leu Lys Gly Val Lys Leu His Tyr Thr 
1265                1270            

<210> 25
<211> 3825
<212> DNA
<213> Artificial Sequence


<220> 
<223> SARS-CoV-2 S protein, full-length, modified, plus signal peptide
      ("SARS-2 S-FS-1")

<400> 25
atgttcgtct tcctggtcct gctccctctg gtgtccagcc agtgtgtgaa cctgaccacc       60

agaacacagc tgcctccagc ctacaccaac agctttacca gaggcgtgta ctatcccgac      120

aaggtgttca gatccagcgt gctgcactct acccaggacc tgttcctgcc tttcttcagc      180

aacgtgacct ggttccacgc catccacgtg agcggtacca atggcaccaa gagattcgac      240

aatcccgtgc tgcccttcaa cgacggagtg tactttgcca gcaccgagaa gtccaacatc      300

atcagaggct ggatcttcgg caccacactg gacagcaaga cccagagcct gctgatcgtg      360

aacaacgcca ccaacgtggt catcaaagtg tgcgagttcc agttctgcaa cgatcccttc      420

ctgggagtct actaccacaa gaacaacaag agctggatgg aaagcgagtt cagagtgtac      480

agctctgcca acaactgcac cttcgagtac gtgtcccagc ctttcctgat ggacctggaa      540

ggcaagcagg gcaatttcaa gaatctccga gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct acagcaagca cacacctatc aacctcgtga gggatctgcc tcagggcttc      660

tctgctctgg aacctctggt ggatctgccc atcggcatca acatcactcg gtttcagaca      720

ctgctggcac tgcacagaag ctacctgaca cctggcgata gcagcagcgg atggacagct      780

ggtgctgcag cttactatgt gggctacctg cagcctagaa ccttcctgct gaagtacaac      840

gagaacggca ccatcaccga tgccgtggat tgtgctctgg atcctctgag cgagacaaag      900

tgcaccctga agtccttcac cgtggagaag ggcatctacc agaccagcaa cttcagggtg      960

cagcccaccg aatccatcgt gcggttccct aacatcacca atctgtgtcc ctttggcgag     1020

gtgttcaatg ccaccagatt cgcctctgtg tacgcctgga accggaagcg gatcagcaat     1080

tgcgttgccg actactccgt gctgtacaac tcagccagct tcagcacctt caagtgctac     1140

ggagtgagcc ctaccaagct gaacgacctg tgcttcacaa acgtgtatgc tgacagcttc     1200

gtgatcagag gagatgaagt gaggcagatt gctcctggac agacaggcaa gattgcagat     1260

tacaactaca agctgcctga cgacttcaca ggctgtgtga ttgcctggaa cagcaacaac     1320

ctggactcca aagtcggagg caactacaac tacctgtaca gactgttcag gaagtccaat     1380

ctgaagccct tcgaacggga catctccacc gagatctatc aggctggcag cacaccttgt     1440

aatggagtgg aaggcttcaa ctgctacttc ccactgcagt cctacggctt tcagcccaca     1500

aatggcgtgg gctatcagcc ctacagagtg gtggtgctga gcttcgaact gctgcatgct     1560

cctgccacag tgtgcggacc taagaaatcc accaatctcg tgaagaacaa atgcgtgaac     1620

ttcaacttca acggtctgac cggtacaggc gtgctgacag agagcaacaa gaagttcctg     1680

ccattccagc agtttggcag ggatatcgca gataccacag acgctgttag agatccacag     1740

acactggaaa tcctggacat cacaccttgc agcttcggag gcgtgtctgt gatcacacct     1800

ggcaccaaca ccagcaatca ggtggcagtt ctgtaccagg acgtgaactg taccgaagtt     1860

cccgtggcca ttcatgccga tcagctgaca cctacatgga gggtgtactc caccggaagc     1920

aatgtgtttc agaccagagc tggctgtctg atcggagccg agcacgtgaa caatagctac     1980

gagtgcgaca tccctatcgg agctggcatc tgtgccagct accagacaca gacaaacagc     2040

cctggctctg cctcttccgt ggccagccag tccatcattg cctacacaat gtctctggga     2100

gccgagaaca gcgtggccta ctccaacaac tctatcgcta tccctaccaa cttcacaatc     2160

agcgtgacca cagagatcct gcctgtgtcc atgaccaaga ccagcgtgga ctgcaccatg     2220

tacatctgtg gcgattccac cgagtgctcc aacctgctgc tgcagtacgg cagcttctgc     2280

acccagctga atagagccct gacagggatc gctgtggaac aggacaagaa cacccaagag     2340

gtgttcgcac aagtgaagca gatctacaag acacctccta tcaaggactt cggaggattc     2400

aactttagcc agattctgcc tgatcctagc aagccaagca agcggagctt catcgaggac     2460

ctgctgttca acaaagtgac acttgccgac gctggcttca tcaagcagta tggcgattgt     2520

ctgggagaca ttgctgccag ggatctgatt tgtgcccaga agttcaacgg actgacagtg     2580

ctgcctcctc tgctgaccga tgagatgatc gctcagtaca catctgccct gctggctggc     2640

acaatcacaa gtggctggac atttggagct ggagctgctc tgcagattcc ctttgctatg     2700

cagatggcct accggttcaa cggcatcgga gtgacccaga atgtgctgta cgagaaccag     2760

aagctgatcg ccaaccagtt caactctgcc atcggcaaga tccaggacag cctgagcagc     2820

acagcaagcg cactgggaaa gctgcaggac gtggtcaacc agaatgccca ggcactgaac     2880

accctggtca agcagctgtc ctccaacttc ggagccatca gctctgtgct gaacgatatc     2940

ctgagcagac tggaccctcc tgaagccgag gtgcagatcg acaggctgat cacaggcaga     3000

ctgcagagcc tccagacata cgtgacccag cagctgatca gagctgccga gattagagcc     3060

tctgccaatc tggcagccac caagatgtct gagtgtgtgc tgggacagag caagagagtg     3120

gacttctgtg gcaagggcta ccacctgatg agcttccctc agtctgcacc tcatggcgtg     3180

gtgtttctgc acgtgacata tgtgccagct caagagaaga acttcaccac cgctccagcc     3240

atctgccacg acggtaaagc ccactttcct agagaaggcg tgttcgtgtc caacggcacc     3300

cattggttcg tgacacagcg gaacttctac gagcctcaga tcatcaccac cgacaacacc     3360

ttcgtgtctg gcaactgcga cgtcgtgatc ggcattgtca acaataccgt gtacgaccct     3420

ctgcagccag agctcgacag cttcaaagag gaactggaca agtacttcaa gaaccacaca     3480

agccctgacg tggacctggg agatatcagc ggaatcaatg ccagcgtcgt gaacatccag     3540

aaagagattg acagactgaa cgaggtggcc aagaatctga acgagagcct gatcgacctg     3600

caagaacttg ggaagtacga gcagtacatc aagtggcctt ggtacatctg gctgggcttc     3660

atcgcaggac tgattgccat cgtgatggtc acaatcatgc tgtgttgcat gaccagctgc     3720

tgtagctgtc tgaagggctg ttgtagctgt ggcagctgct gcaagttcga cgaggacgat     3780

tctgagccag tgctgaaagg cgtgaaactg cactacacat agtaa                     3825


<210> 26
<211> 124
<212> DNA
<213> Artificial Sequence


<220> 
<223> Pr13.5long promoter

<400> 26
taaaaataga aactataatc atataatagt gtaggttggt agtattgctc ttgtgactag     60

agactttagt taaggtactg taaaaataga aactataatc atataatagt gtaggttggt    120

agta                                                                 124


<210> 27
<211> 100
<212> DNA
<213> Artificial Sequence


<220> 
<223> Pr1328 promoter

<400> 27
tatattatta agtgtggtgt ttggtcgatg taaaattttt gtcgataaaa attaaaaaat     60

aacttaattt attattgatc tcgtgtgtac aaccgaaatc                          100


