                         SEQUENCE LISTING

<110>  Nanjing GenScript Biotech Co., Ltd.(Ͼ˹Ƽ޹˾)
 
<120>  barstarĹ̾barnase¡еӦ

<130>  RS-C20202P1

<150>  CN202010534573.8
<151>  2020-06-12

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  276
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  barstar

<400>  1
atgaagaaag cggtgatcaa cggcgagcaa atccgtagca ttagcgacct gcaccagacc       60

ctgaagaaag aactggcgct gccggagtac tatggtgaaa acctggacgc gctgtgggat      120

gcgctgaccg gttgggtgga gtacccgctg gttctggagt ggcgtcagtt cgaacaaagc      180

aagcagctga ccgagaacgg tgcggaaagc gtgctgcaag tttttcgtga ggcgaaagcg      240

gaaggcgcgg atattaccat cattctgagc taataa                                276


<210>  2
<211>  505
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  barstarԪ

<400>  2
gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac       60

taaaccattt aataggaggt agttgatgaa gaaagcggtg atcaacggcg agcaaatccg      120

tagcattagc gacctgcacc agaccctgaa gaaagaactg gcgctgccgg agtactatgg      180

tgaaaacctg gacgcgctgt gggatgcgct gaccggttgg gtggagtacc cgctggttct      240

ggagtggcgt cagttcgaac aaagcaagca gctgaccgag aacggtgcgg aaagcgtgct      300

gcaagttttt cgtgaggcga aagcggaagg cgcggatatt accatcattc tgagctaata      360

ataaccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc      420

tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct      480

ttctgcgttt atactgtaca agtag                                            505


<210>  3
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  barnase

<400>  3
atggcacagg ttatcaacac gtttgacggg gttgcggatt atcttcagac atatcataag       60

ctacctgata attacattac aaaatcagaa gcacaagccc tcggctgggt ggcatcaaaa      120

gggaaccttg cagacgtcgc tccggggaaa agcatcggcg gagacatctt ctcaaacagg      180

gaaggcaaac tcccgggcaa aagcggacga acatggcgtg aagcggatat taactataca      240

tcaggcttca gaaattcaga ccggattctt tactcaagcg actggctgat ttacaaaaca      300

acggaccatt atcagacctt tacaaaaatc agataa                                336


<210>  4
<211>  5530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U9041-1ȫ

<400>  4
ggcatgcaag ctgatttggc tgctgccacc gctgagcaag gccgcttcga gcagacatga       60

taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta      120

tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag      180

ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt      240

ttttaagcaa gtaaaacctc tacaaatgtg gtaaaatctt aattaagaat tctaatataa      300

cttcgtatag catacattat acgaagttat ggatccactg ttgacaatta atcatcggca      360

tagtatatcg gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat      420

gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg      480

ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc      540

gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca      600

ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct      660

cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga      720

tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg      780

gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat      840

cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga      900

gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg      960

cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg     1020

ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat     1080

agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct     1140

cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga     1200

cgagttcttc tgattcgaaa tgaccgacca agcgacgccc aaccggtatc agctcactca     1260

aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca     1320

aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg     1380

ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg     1440

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     1500

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     1560

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     1620

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     1680

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     1740

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     1800

tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa     1860

agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt     1920

tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt gatcttttct     1980

acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta     2040

tcaaaaagga tcttcaccta gatcctttta tagtccggaa tcgtcacaca aaaaaccaac     2100

acacagatgt aatgaaaata aagatatttt attgcggcca tcgtgatggc tagagtttag     2160

ccctcccaca cataaccaga gggcagcaat tcacgaatcc caactgccgt cggctgtcca     2220

tcactgtcct tcactatggc tttgatccca ggatgcagat cgagaagcac ctgtcggcac     2280

cgtccgcagg ggctcaagat gcccctgttc tcatttccga tcgcgacgat acaagtcagg     2340

ttgccagctg ccgcagcagc agcagtgccc agcaccacga gttctgcaca aggtccccca     2400

gtaaaatgat atacattgac accagtgaag atgcggccgt cgctagagag agctgcgctg     2460

gcgacgctgt agtcttcaga gatggggatg ctgttgattg tagccgttgc tctttcaatg     2520

agggtggatt cttcttgaga caaaggcttg gccatggtgg cgctagtgtc agaagaatcg     2580

agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca     2640

gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga     2700

gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg     2760

gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac     2820

tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg     2880

gagcctgggg actttccaca ccctaactga cacacattcc acagctggtt ctttccgcct     2940

cagaaggtac ctaaccaagt tcctctttca gaggttattt caggccatgg tgctgcgcaa     3000

gacgcgttaa ttaagacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc     3060

tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat     3120

tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct     3180

ctacaaatgt ggtatggctg attatgatcc gcggccgcaa tacgtcgacg ttatcagctg     3240

acttcgtacg agagcctagg attatggcgc gccactagtt attaatagta atcaattacg     3300

gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc     3360

ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc     3420

atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact     3480

gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat     3540

gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact     3600

tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac     3660

accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac     3720

gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac     3780

cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga     3840

gctggtttag tgaaccgtca gatcactaga agctttattg cggtagttta tcacagttaa     3900

attgctaacg cagtcagtgc ttctgacaca acagtctcga acttaagctg cagaagttgg     3960

tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag     4020

aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt     4080

actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct     4140

taaggctaga gtattaatac gactcactat agggctagcg atcgccatgg aataagtaag     4200

gaatccacat ggcacaggtt atcaacacgt ttgacggggt tgcggattat cttcagacat     4260

atcataagct acctgataat tacattacaa aatcagaagc acaagccctc ggctgggtgg     4320

catcaaaagg gaaccttgca gacgtcgctc cggggaaaag catcggcgga gacatcttct     4380

caaacaggga aggcaaactc ccgggcaaaa gcggacgaac atggcgtgaa gcggatatta     4440

actatacatc aggcttcaga aattcagacc ggattcttta ctcaagcgac tggctgattt     4500

acaaaacaac ggaccattat cagaccttta caaaaatcag ataatgttta atgaccccgt     4560

gtcgagctct cgagccaacc actgaggatc tgtactttca gagcgataac gatggatccg     4620

aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc gagcgcatgc     4680

actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac ggtaacccga     4740

cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat cgctgcattg     4800

ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat ttcttcgacg     4860

accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag gtcgtcctgg     4920

tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat ccagagcgcg     4980

tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac gaatggccag     5040

aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc aagctgatca     5100

tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg     5160

aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt     5220

ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg     5280

aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc tggggcaccc     5340

caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg cctaactgca     5400

aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg gacctgatcg     5460

gcagcgagat cgcgcgctgg ctgtctactc tggagatttc cggttaatag aattctagag     5520

tcgacctgca                                                            5530


<210>  5
<211>  4687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U9041-12ȫ

<400>  5
aagacgcgtt aattaagacg tcggcagtga aaaaaatgct ttatttgtga aatttgtgat       60

gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc      120

attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac      180

ctctacaaat gtggtatggc tgattatgat ccgcggccgc aatacgtcga cgttatcagc      240

tgacttcgta cgagagccta ggattatggc gcgccactag ttattaatag taatcaatta      300

cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg      360

gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc      420

ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa      480

ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct attgacgtca      540

atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg gactttccta      600

cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt      660

acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg      720

acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaata      780

accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca      840

gagctggttt agtgaaccgt cagatcacta gaagctttat tgcggtagtt tatcacagtt      900

aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt      960

ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat     1020

agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc     1080

ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct     1140

cttaaggcta gagtattaat acgactcact atagggctag cgctcaccat ggtgaccggc     1200

taccggctgt tcgaggagat tctcgggagc tccggtggtg gcgggagcgg aggtggaggc     1260

tcgagcggtg cgatcgccat ggaataagta aggaatccac atggcacagg ttatcaacac     1320

gtttgacggg gttgcggatt atcttcagac atatcataag ctacctgata attacattac     1380

aaaatcagaa gcacaagccc tcggctgggt ggcatcaaaa gggaaccttg cagacgtcgc     1440

tccggggaaa agcatcggcg gagacatctt ctcaaacagg gaaggcaaac tcccgggcaa     1500

aagcggacga acatggcgtg aagcggatat taactataca tcaggcttca gaaattcaga     1560

ccggattctt tactcaagcg actggctgat ttacaaaaca acggaccatt atcagacctt     1620

tacaaaaatc agataatgtt taaacgaatt cgggctcggt acccggggat cctctagagt     1680

cgacctgcag gcatgcaagc tgatttggct gctgccaccg ctgagcaagg ccgcttcgag     1740

cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa     1800

aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca     1860

ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt     1920

gggaggtttt tttaagcaag taaaacctct acaaatgtgg taaaatctta attaagaatt     1980

ctaatataac ttcgtatagc atacattata cgaagttatg gatccactgt tgacaattaa     2040

tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aacccaggag     2100

gcagatcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag     2160

gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg     2220

gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa     2280

tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc     2340

agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc     2400

ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga     2460

tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa     2520

acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct     2580

ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat     2640

gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt     2700

ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta     2760

tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga     2820

ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg     2880

ccttcttgac gagttcttct gattcgaaat gaccgaccaa gcgacgccca accggtatca     2940

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     3000

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     3060

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     3120

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     3180

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     3240

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     3300

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     3360

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     3420

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     3480

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc     3540

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     3600

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga agatcctttg     3660

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     3720

atgagattat caaaaaggat cttcacctag atccttttat agtccggaat cgtcacacaa     3780

aaaaccaaca cacagatgta atgaaaataa agatatttta ttgcggccat cgtgatggct     3840

agagtttagc cctcccacac ataaccagag ggcagcaatt cacgaatccc aactgccgtc     3900

ggctgtccat cactgtcctt cactatggct ttgatcccag gatgcagatc gagaagcacc     3960

tgtcggcacc gtccgcaggg gctcaagatg cccctgttct catttccgat cgcgacgata     4020

caagtcaggt tgccagctgc cgcagcagca gcagtgccca gcaccacgag ttctgcacaa     4080

ggtcccccag taaaatgata tacattgaca ccagtgaaga tgcggccgtc gctagagaga     4140

gctgcgctgg cgacgctgta gtcttcagag atggggatgc tgttgattgt agccgttgct     4200

ctttcaatga gggtggattc ttcttgagac aaaggcttgg ccatggtggc gctagtgtca     4260

gaagaatcga gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg     4320

aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca     4380

tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc     4440

gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag     4500

cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg     4560

cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc     4620

tttccgcctc agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt     4680

gctgcgc                                                               4687


<210>  6
<211>  2961
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC57-barnaseȫ

<400>  6
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa      420

tacatctaga tatcgcgatc gccatggaat aagtaaggaa tccacatggc acaggttatc      480

aacacgtttg acggggttgc ggattatctt cagacatatc ataagctacc tgataattac      540

attacaaaat cagaagcaca agccctcggc tgggtggcat caaaagggaa ccttgcagac      600

gtcgctccgg ggaaaagcat cggcggagac atcttctcaa acagggaagg caaactcccg      660

ggcaaaagcg gacgaacatg gcgtgaagcg gatattaact atacatcagg cttcagaaat      720

tcagaccgga ttctttactc aagcgactgg ctgatttaca aaacaacgga ccattatcag      780

acctttacaa aaatcagata atgtttaaac gatatcggat cccgggcccg tcgactgcag      840

aggcctgcat gcaagcttgg tgtaatcatg gtcatagctg tttcctgtgt gaaattgtta      900

tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc      960

ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg     1020

aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg     1080

tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg     1140

gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa     1200

cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc     1260

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     1320

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     1380

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     1440

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     1500

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     1560

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     1620

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     1680

gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct     1740

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     1800

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     1860

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     1920

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     1980

atgaagtttt aaatcaagcc caatctgaat aatgttacaa ccaattaacc aattctgatt     2040

agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac     2100

catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata     2160

ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta     2220

ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg     2280

aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc     2340

cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg     2400

cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat     2460

gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt     2520

cttctaatac ctggaatgct gtttttccgg ggatcgcagt ggtgagtaac catgcatcat     2580

caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta     2640

gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca     2700

actctggcgc atcgggcttc ccatacaagc gatagattgt cgcacctgat tgcccgacat     2760

tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc     2820

tcgacgtttc ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag     2880

acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt     2940

gagacacggg ccagagctgc a                                               2961


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-1

<400>  7
gccggaaagc gaggcttatc                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-2

<400>  8
gccggataag cctcgctttc                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA-3

<400>  9
tttccgctaa gattgcatgc                                                   20


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9-F

<400>  10
actagccagc atccgtttac ga                                                22


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9-R

<400>  11
tgcgcaaagt attgtccatg cc                                                22


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  F1

<400>  12
attctcggca aaatgcatca ggacg                                             25


<210>  13
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R1

<400>  13
ttatgaagtc tgaaaagtga ggaaa                                             25


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R2

<400>  14
tcttcatcaa ctacctccta t                                                 21


