                         SEQUENCE LISTING

<110>  Hangzhou Leading Edge Pharmaceutical Ltd.

<120>  Compositions and methods for enhancing immune response

<130>  0171-PA-004

<160>  25

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 1

<400>  1
gaacggtatt gtcggtattg tca                                               23


<210>  2
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 2

<400>  2
agaccactcc aacacagaac tt                                                22


<210>  3
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 3

<400>  3
gttctttgta tatattgaat gttagcc                                           27


<210>  4
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 4

<400>  4
tatgctctaa ggacaagaat tgaca                                             25


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer 5

<400>  5
ctttgcagag ggctaacagc                                                   20


<210>  6
<211>  267
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  scFv

<400>  6

Ala Ala Thr Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu 
1               5                   10                  15      


Trp Val Pro Gly Ser Thr Gly Thr Gly Gln Ile Val Leu Ser Gln Ser 
            20                  25                  30          


Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys 
        35                  40                  45              


Arg Ala Ser Ser Ser Val Ser Tyr Ile His Trp Phe Gln Gln Lys Pro 
    50                  55                  60                  


Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser 
65                  70                  75                  80  


Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
                85                  90                  95      


Leu Thr Ile Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
            100                 105                 110         


Gln Gln Trp Thr Ser Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro 
145                 150                 155                 160 


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                165                 170                 175     


Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu 
            180                 185                 190         


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 
        195                 200                 205             


Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 
    210                 215                 220                 


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
225                 230                 235                 240 


Tyr Cys Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val 
                245                 250                 255     


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ala 
            260                 265         


<210>  7
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ISD

<400>  7

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  8
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3zeta

<400>  8

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  9
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hinge

<400>  9

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  10
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TD

<400>  10

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr Leu Tyr Cys 
            20                  


<210>  11
<211>  493
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  20 CAR

<400>  11

Ala Ala Thr Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu 
1               5                   10                  15      


Trp Val Pro Gly Ser Thr Gly Thr Gly Gln Ile Val Leu Ser Gln Ser 
            20                  25                  30          


Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys 
        35                  40                  45              


Arg Ala Ser Ser Ser Val Ser Tyr Ile His Trp Phe Gln Gln Lys Pro 
    50                  55                  60                  


Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser 
65                  70                  75                  80  


Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
                85                  90                  95      


Leu Thr Ile Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
            100                 105                 110         


Gln Gln Trp Thr Ser Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro 
145                 150                 155                 160 


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                165                 170                 175     


Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu 
            180                 185                 190         


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 
        195                 200                 205             


Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 
    210                 215                 220                 


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
225                 230                 235                 240 


Tyr Cys Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val 
                245                 250                 255     


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ala Ala Ala Ala Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490             


<210>  12
<211>  1479
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20 CAR

<400>  12
gccgccacca tggagaccga caccctctta ttatgggtgc tgttattatg ggtccccgga       60

agcaccggaa ccggtcagat cgtgctgagc cagagccccg ccattctgtc cgccagcccc      120

ggcgagaagg tgaccatgac atgcagagcc tcctcctccg tgagctacat ccactggttc      180

cagcagaagc ccggctcctc ccctaagcct tggatctacg ccaccagcaa tctggctagc      240

ggagtgcccg tgagattcag cggcagcgga agcggaacca gctactctct gaccatcagc      300

agagtggagg ccgaggacgc cgctacctac tactgtcagc agtggaccag caaccctcct      360

accttcggcg gcggcaccaa gctggagatc aagggcggag gaggctccgg aggtggaggt      420

tctggcggtg gaggttccca agtgcagctg cagcagcccg gcgctgagct ggtgaaaccc      480

ggcgcttccg tgaagatgag ctgcaaggcc agcggctaca ccttcaccag ctacaacatg      540

cactgggtga agcagacccc cggcagagga ctggaatgga tcggcgccat ttaccccggc      600

aacggcgata cctcctacaa ccagaagttc aagggcaagg ctacactgac cgccgacaag      660

agcagcagca ccgcctacat gcagctgagc tctctgacca gcgaggacag cgccgtgtac      720

tactgcgcta gaagcaccta ctacggcggc gactggtact tcaacgtgtg gggagccggc      780

acaaccgtga cagtgtccgc cgcggccgct acaaccaccc ccgctcccag acctcctaca      840

cccgctccca ccattgccag ccagcctctc tctttaagac ccgaggcttg taggcccgct      900

gctggaggag ccgtgcacac aaggggactg gactttgctt gtgatatcta tatctgggcc      960

cctctggctg gaacttgtgg agtcctctta ttatctttag tgatcacttt atactgtaag     1020

aggggtcgta agaagttatt atacatcttc aagcagccct tcatgaggcc cgtccaaacc     1080

acccaagaag aggacggatg tagctgtagg tttcccgagg aggaggaggg aggctgcgaa     1140

ttacgtgtca agttctccag aagcgccgat gcccccgctt accaacaagg tcagaaccag     1200

ctgtacaatg agctgaatct gggcagaaga gaagagtacg acgtgctgga taagaggagg     1260

ggtcgtgacc ccgaaatggg aggcaagccc agaagaaaaa acccccaaga aggactctac     1320

aacgagctgc aaaaggataa gatggctgag gcctattccg agattggcat gaagggcgag     1380

agaaggagag gcaagggcca cgacggttta tatcaaggtc tctccaccgc caccaaggac     1440

acatacgatg ctctgcacat gcaagctctg ccccccaga                            1479


<210>  13
<211>  3291
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  20-Y CAR

<400>  13
gccgccacca tggagaccga caccctctta ttatgggtgc tgttattatg ggtccccgga       60

agcaccggaa ccggtcagat cgtgctgagc cagagccccg ccattctgtc cgccagcccc      120

ggcgagaagg tgaccatgac atgcagagcc tcctcctccg tgagctacat ccactggttc      180

cagcagaagc ccggctcctc ccctaagcct tggatctacg ccaccagcaa tctggctagc      240

ggagtgcccg tgagattcag cggcagcgga agcggaacca gctactctct gaccatcagc      300

agagtggagg ccgaggacgc cgctacctac tactgtcagc agtggaccag caaccctcct      360

accttcggcg gcggcaccaa gctggagatc aagggcggag gaggctccgg aggtggaggt      420

tctggcggtg gaggttccca agtgcagctg cagcagcccg gcgctgagct ggtgaaaccc      480

ggcgcttccg tgaagatgag ctgcaaggcc agcggctaca ccttcaccag ctacaacatg      540

cactgggtga agcagacccc cggcagagga ctggaatgga tcggcgccat ttaccccggc      600

aacggcgata cctcctacaa ccagaagttc aagggcaagg ctacactgac cgccgacaag      660

agcagcagca ccgcctacat gcagctgagc tctctgacca gcgaggacag cgccgtgtac      720

tactgcgcta gaagcaccta ctacggcggc gactggtact tcaacgtgtg gggagccggc      780

acaaccgtga cagtgtccgc cgcggccgct acaaccaccc ccgctcccag acctcctaca      840

cccgctccca ccattgccag ccagcctctc tctttaagac ccgaggcttg taggcccgct      900

gctggaggag ccgtgcacac aaggggactg gactttgctt gtgatatcta tatctgggcc      960

cctctggctg gaacttgtgg agtcctctta ttatctttag tgatcacttt atactgtaag     1020

aggggtcgta agaagttatt atacatcttc aagcagccct tcatgaggcc cgtccaaacc     1080

acccaagaag aggacggatg tagctgtagg tttcccgagg aggaggaggg aggctgcgaa     1140

ttacgtgtca agttctccag aagcgccgat gcccccgctt accaacaagg tcagaaccag     1200

ctgtacaatg agctgaatct gggcagaaga gaagagtacg acgtgctgga taagaggagg     1260

ggtcgtgacc ccgaaatggg aggcaagccc agaagaaaaa acccccaaga aggactctac     1320

aacgagctgc aaaaggataa gatggctgag gcctattccg agattggcat gaagggcgag     1380

agaaggagag gcaagggcca cgacggttta tatcaaggtc tctccaccgc caccaaggac     1440

acatacgatg ctctgcacat gcaagctctg ccccccagag gctccggcgc cacaaacttc     1500

tctttactga agcaagctgg agacgtggag gagaaccccg gtcctcgtac gatgtcggcc     1560

agcagcctct tggagcagag accaaaaggt caaggaaaca aagtacaaaa tggatctgta     1620

catcaaaagg atggattaaa cgatgatgat tttgaacctt acttgagtcc acaggcaagg     1680

cccaataatg catatactgc catgtcagat tcctacttac ccagttacta cagtccctcc     1740

attggcttct cctattcttt gggtgaagct gcttggtcta cggggggtga cacagccatg     1800

ccctacttaa cttcttatgg acagctgagc aacggagagc cccacttcct accagatgca     1860

atgtttgggc aaccaggagc cctaggtagc actccatttc ttggtcagca tggttttaat     1920

ttctttccca gtgggattga cttctcagca tggggaaata acagttctca gggacagtct     1980

actcagagct ctggatatag tagcaattat gcttatgcac ctagctcctt aggtggagcc     2040

atgattgatg gacagtcagc ttttgccaat gagaccctca ataaggctcc tggcatgaat     2100

actatagacc aagggatggc agcactgaag ttgggtagca cagaagttgc aagcaatgtt     2160

ccaaaagttg taggttctgc tgttggtagc gggtccatta ctagtaacat cgtggcttcc     2220

aatagtttgc ctccagccac cattgctcct ccaaaaccag catcttgggc tgatattgct     2280

agcaagcctg caaaacagca acctaaactg aagaccaaga atggcattgc agggtcaagt     2340

cttccgccac ccccgataaa gcataacatg gatattggaa cttgggataa caagggtccc     2400

gttgcaaaag ccccctcaca ggctttggtt cagaatatag gtcagccaac ccaggggtct     2460

cctcagcctg taggtcagca ggctaacaat agcccaccag tggctcaggc atcagtaggg     2520

caacagacac agccattgcc tccacctcca ccacagcctg cccagctttc agtccagcaa     2580

caggcagctc agccaacccg ctgggtagca cctcggaacc gtggcagtgg gttcggtcat     2640

aatggggtgg atggtaatgg agtaggacag tctcaggctg gttctggatc tactccttca     2700

gaaccccacc cagtgttgga gaagcttcgg tccattaata actataaccc caaagatttt     2760

gactggaatc tgaaacatgg ccgggttttc atcattaaga gctactctga ggacgatatt     2820

caccgttcca ttaagtataa tatttggtgc agcacagagc atggtaacaa gagactggat     2880

gctgcttatc gttccatgaa cgggaaaggc cccgtttact tacttttcag tgtcaacggc     2940

agtggacact tctgtggcgt ggcagaaatg aaatctgctg tggactacaa cacatgtgca     3000

ggtgtgtggt cccaggacaa atggaagggt cgttttgatg tcaggtggat ttttgtgaag     3060

gacgttccca atagccaact gcgacacatt cgcctagaga acaacgagaa taaaccagtg     3120

accaactcta gggacactca ggaagtgcct ctggaaaagg ctaagcaggt gttgaaaatt     3180

atagccagct acaagcacac cacttccatt tttgatgact tctcacacta tgagaaacgc     3240

caagaggaag aagaaagtgt taaaaaggaa cgtcaaggtc gtgggaaata a              3291


<210>  14
<211>  1096
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  20-Y

<400>  14

Ala Ala Thr Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu 
1               5                   10                  15      


Trp Val Pro Gly Ser Thr Gly Thr Gly Gln Ile Val Leu Ser Gln Ser 
            20                  25                  30          


Pro Ala Ile Leu Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys 
        35                  40                  45              


Arg Ala Ser Ser Ser Val Ser Tyr Ile His Trp Phe Gln Gln Lys Pro 
    50                  55                  60                  


Gly Ser Ser Pro Lys Pro Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser 
65                  70                  75                  80  


Gly Val Pro Val Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser 
                85                  90                  95      


Leu Thr Ile Ser Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys 
            100                 105                 110         


Gln Gln Trp Thr Ser Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu 
        115                 120                 125             


Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gln Val Gln Leu Gln Gln Pro Gly Ala Glu Leu Val Lys Pro 
145                 150                 155                 160 


Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr 
                165                 170                 175     


Ser Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Arg Gly Leu Glu 
            180                 185                 190         


Trp Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln 
        195                 200                 205             


Lys Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr 
    210                 215                 220                 


Ala Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr 
225                 230                 235                 240 


Tyr Cys Ala Arg Ser Thr Tyr Tyr Gly Gly Asp Trp Tyr Phe Asn Val 
                245                 250                 255     


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ala Ala Ala Ala Thr Thr 
            260                 265                 270         


Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln 
        275                 280                 285             


Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala 
    290                 295                 300                 


Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala 
305                 310                 315                 320 


Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr 
                325                 330                 335     


Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln 
            340                 345                 350         


Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser 
        355                 360                 365             


Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys 
    370                 375                 380                 


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
385                 390                 395                 400 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
                405                 410                 415     


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
            420                 425                 430         


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
        435                 440                 445             


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
    450                 455                 460                 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
465                 470                 475                 480 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Gly Ser Gly 
                485                 490                 495     


Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
            500                 505                 510         


Pro Gly Pro Arg Thr Met Ser Ala Ser Ser Leu Leu Glu Gln Arg Pro 
        515                 520                 525             


Lys Gly Gln Gly Asn Lys Val Gln Asn Gly Ser Val His Gln Lys Asp 
    530                 535                 540                 


Gly Leu Asn Asp Asp Asp Phe Glu Pro Tyr Leu Ser Pro Gln Ala Arg 
545                 550                 555                 560 


Pro Asn Asn Ala Tyr Thr Ala Met Ser Asp Ser Tyr Leu Pro Ser Tyr 
                565                 570                 575     


Tyr Ser Pro Ser Ile Gly Phe Ser Tyr Ser Leu Gly Glu Ala Ala Trp 
            580                 585                 590         


Ser Thr Gly Gly Asp Thr Ala Met Pro Tyr Leu Thr Ser Tyr Gly Gln 
        595                 600                 605             


Leu Ser Asn Gly Glu Pro His Phe Leu Pro Asp Ala Met Phe Gly Gln 
    610                 615                 620                 


Pro Gly Ala Leu Gly Ser Thr Pro Phe Leu Gly Gln His Gly Phe Asn 
625                 630                 635                 640 


Phe Phe Pro Ser Gly Ile Asp Phe Ser Ala Trp Gly Asn Asn Ser Ser 
                645                 650                 655     


Gln Gly Gln Ser Thr Gln Ser Ser Gly Tyr Ser Ser Asn Tyr Ala Tyr 
            660                 665                 670         


Ala Pro Ser Ser Leu Gly Gly Ala Met Ile Asp Gly Gln Ser Ala Phe 
        675                 680                 685             


Ala Asn Glu Thr Leu Asn Lys Ala Pro Gly Met Asn Thr Ile Asp Gln 
    690                 695                 700                 


Gly Met Ala Ala Leu Lys Leu Gly Ser Thr Glu Val Ala Ser Asn Val 
705                 710                 715                 720 


Pro Lys Val Val Gly Ser Ala Val Gly Ser Gly Ser Ile Thr Ser Asn 
                725                 730                 735     


Ile Val Ala Ser Asn Ser Leu Pro Pro Ala Thr Ile Ala Pro Pro Lys 
            740                 745                 750         


Pro Ala Ser Trp Ala Asp Ile Ala Ser Lys Pro Ala Lys Gln Gln Pro 
        755                 760                 765             


Lys Leu Lys Thr Lys Asn Gly Ile Ala Gly Ser Ser Leu Pro Pro Pro 
    770                 775                 780                 


Pro Ile Lys His Asn Met Asp Ile Gly Thr Trp Asp Asn Lys Gly Pro 
785                 790                 795                 800 


Val Ala Lys Ala Pro Ser Gln Ala Leu Val Gln Asn Ile Gly Gln Pro 
                805                 810                 815     


Thr Gln Gly Ser Pro Gln Pro Val Gly Gln Gln Ala Asn Asn Ser Pro 
            820                 825                 830         


Pro Val Ala Gln Ala Ser Val Gly Gln Gln Thr Gln Pro Leu Pro Pro 
        835                 840                 845             


Pro Pro Pro Gln Pro Ala Gln Leu Ser Val Gln Gln Gln Ala Ala Gln 
    850                 855                 860                 


Pro Thr Arg Trp Val Ala Pro Arg Asn Arg Gly Ser Gly Phe Gly His 
865                 870                 875                 880 


Asn Gly Val Asp Gly Asn Gly Val Gly Gln Ser Gln Ala Gly Ser Gly 
                885                 890                 895     


Ser Thr Pro Ser Glu Pro His Pro Val Leu Glu Lys Leu Arg Ser Ile 
            900                 905                 910         


Asn Asn Tyr Asn Pro Lys Asp Phe Asp Trp Asn Leu Lys His Gly Arg 
        915                 920                 925             


Val Phe Ile Ile Lys Ser Tyr Ser Glu Asp Asp Ile His Arg Ser Ile 
    930                 935                 940                 


Lys Tyr Asn Ile Trp Cys Ser Thr Glu His Gly Asn Lys Arg Leu Asp 
945                 950                 955                 960 


Ala Ala Tyr Arg Ser Met Asn Gly Lys Gly Pro Val Tyr Leu Leu Phe 
                965                 970                 975     


Ser Val Asn Gly Ser Gly His Phe Cys Gly Val Ala Glu Met Lys Ser 
            980                 985                 990         


Ala Val Asp Tyr Asn Thr Cys Ala Gly Val Trp Ser Gln Asp Lys Trp 
        995                 1000                1005            


Lys Gly Arg Phe Asp Val Arg Trp Ile Phe Val Lys Asp Val Pro Asn 
    1010                1015                1020                


Ser Gln Leu Arg His Ile Arg Leu Glu Asn Asn Glu Asn Lys Pro Val 
1025                1030                1035                1040


Thr Asn Ser Arg Asp Thr Gln Glu Val Pro Leu Glu Lys Ala Lys Gln 
                1045                1050                1055    


Val Leu Lys Ile Ile Ala Ser Tyr Lys His Thr Thr Ser Ile Phe Asp 
            1060                1065                1070        


Asp Phe Ser His Tyr Glu Lys Arg Gln Glu Glu Glu Glu Ser Val Lys 
        1075                1080                1085            


Lys Glu Arg Gln Gly Arg Gly Lys 
    1090                1095    


<210>  15
<211>  579
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Y

<400>  15

Met Ser Ala Ser Ser Leu Leu Glu Gln Arg Pro Lys Gly Gln Gly Asn 
1               5                   10                  15      


Lys Val Gln Asn Gly Ser Val His Gln Lys Asp Gly Leu Asn Asp Asp 
            20                  25                  30          


Asp Phe Glu Pro Tyr Leu Ser Pro Gln Ala Arg Pro Asn Asn Ala Tyr 
        35                  40                  45              


Thr Ala Met Ser Asp Ser Tyr Leu Pro Ser Tyr Tyr Ser Pro Ser Ile 
    50                  55                  60                  


Gly Phe Ser Tyr Ser Leu Gly Glu Ala Ala Trp Ser Thr Gly Gly Asp 
65                  70                  75                  80  


Thr Ala Met Pro Tyr Leu Thr Ser Tyr Gly Gln Leu Ser Asn Gly Glu 
                85                  90                  95      


Pro His Phe Leu Pro Asp Ala Met Phe Gly Gln Pro Gly Ala Leu Gly 
            100                 105                 110         


Ser Thr Pro Phe Leu Gly Gln His Gly Phe Asn Phe Phe Pro Ser Gly 
        115                 120                 125             


Ile Asp Phe Ser Ala Trp Gly Asn Asn Ser Ser Gln Gly Gln Ser Thr 
    130                 135                 140                 


Gln Ser Ser Gly Tyr Ser Ser Asn Tyr Ala Tyr Ala Pro Ser Ser Leu 
145                 150                 155                 160 


Gly Gly Ala Met Ile Asp Gly Gln Ser Ala Phe Ala Asn Glu Thr Leu 
                165                 170                 175     


Asn Lys Ala Pro Gly Met Asn Thr Ile Asp Gln Gly Met Ala Ala Leu 
            180                 185                 190         


Lys Leu Gly Ser Thr Glu Val Ala Ser Asn Val Pro Lys Val Val Gly 
        195                 200                 205             


Ser Ala Val Gly Ser Gly Ser Ile Thr Ser Asn Ile Val Ala Ser Asn 
    210                 215                 220                 


Ser Leu Pro Pro Ala Thr Ile Ala Pro Pro Lys Pro Ala Ser Trp Ala 
225                 230                 235                 240 


Asp Ile Ala Ser Lys Pro Ala Lys Gln Gln Pro Lys Leu Lys Thr Lys 
                245                 250                 255     


Asn Gly Ile Ala Gly Ser Ser Leu Pro Pro Pro Pro Ile Lys His Asn 
            260                 265                 270         


Met Asp Ile Gly Thr Trp Asp Asn Lys Gly Pro Val Ala Lys Ala Pro 
        275                 280                 285             


Ser Gln Ala Leu Val Gln Asn Ile Gly Gln Pro Thr Gln Gly Ser Pro 
    290                 295                 300                 


Gln Pro Val Gly Gln Gln Ala Asn Asn Ser Pro Pro Val Ala Gln Ala 
305                 310                 315                 320 


Ser Val Gly Gln Gln Thr Gln Pro Leu Pro Pro Pro Pro Pro Gln Pro 
                325                 330                 335     


Ala Gln Leu Ser Val Gln Gln Gln Ala Ala Gln Pro Thr Arg Trp Val 
            340                 345                 350         


Ala Pro Arg Asn Arg Gly Ser Gly Phe Gly His Asn Gly Val Asp Gly 
        355                 360                 365             


Asn Gly Val Gly Gln Ser Gln Ala Gly Ser Gly Ser Thr Pro Ser Glu 
    370                 375                 380                 


Pro His Pro Val Leu Glu Lys Leu Arg Ser Ile Asn Asn Tyr Asn Pro 
385                 390                 395                 400 


Lys Asp Phe Asp Trp Asn Leu Lys His Gly Arg Val Phe Ile Ile Lys 
                405                 410                 415     


Ser Tyr Ser Glu Asp Asp Ile His Arg Ser Ile Lys Tyr Asn Ile Trp 
            420                 425                 430         


Cys Ser Thr Glu His Gly Asn Lys Arg Leu Asp Ala Ala Tyr Arg Ser 
        435                 440                 445             


Met Asn Gly Lys Gly Pro Val Tyr Leu Leu Phe Ser Val Asn Gly Ser 
    450                 455                 460                 


Gly His Phe Cys Gly Val Ala Glu Met Lys Ser Ala Val Asp Tyr Asn 
465                 470                 475                 480 


Thr Cys Ala Gly Val Trp Ser Gln Asp Lys Trp Lys Gly Arg Phe Asp 
                485                 490                 495     


Val Arg Trp Ile Phe Val Lys Asp Val Pro Asn Ser Gln Leu Arg His 
            500                 505                 510         


Ile Arg Leu Glu Asn Asn Glu Asn Lys Pro Val Thr Asn Ser Arg Asp 
        515                 520                 525             


Thr Gln Glu Val Pro Leu Glu Lys Ala Lys Gln Val Leu Lys Ile Ile 
    530                 535                 540                 


Ala Ser Tyr Lys His Thr Thr Ser Ile Phe Asp Asp Phe Ser His Tyr 
545                 550                 555                 560 


Glu Lys Arg Gln Glu Glu Glu Glu Ser Val Lys Lys Glu Arg Gln Gly 
                565                 570                 575     


Arg Gly Lys 
            


<210>  16
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  16

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target

<400>  17
tgaaccttac ttgagtccac                                                   20


<210>  18
<211>  67
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  tracrRNA

<400>  18
aaauagcaag uuaaaauaag gcuaguccgu uaucaacuug aaaaaguggc accgagucgg       60

ugcuuuu                                                                 67


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  19

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  20
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A

<400>  20

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210>  21
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A

<400>  21

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210>  22
<211>  271
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CLDN18.2 scFv

<400>  22

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Thr Gly Asp Ile Val Met Thr Gln Ser Pro Asp Ser 
            20                  25                  30          


Leu Ala Val Ser Leu Gly Glu Arg Ala Thr Ile Ser Cys Lys Ser Ser 
        35                  40                  45              


Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr 
    50                  55                  60                  


Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser 
65                  70                  75                  80  


Thr Arg Glu Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly 
                85                  90                  95      


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Ala Glu Asp Val Ala 
            100                 105                 110         


Val Tyr Tyr Cys Gln Asn Asp Tyr Phe Tyr Pro Phe Thr Phe Gly Gln 
        115                 120                 125             


Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln Leu Val Gln Ser 
145                 150                 155                 160 


Gly Ala Glu Val Lys Lys Pro Gly Ser Ser Val Lys Val Ser Cys Lys 
                165                 170                 175     


Ala Ser Gly Tyr Ala Phe Ser Asn Tyr Leu Ile Glu Trp Val Lys Gln 
            180                 185                 190         


Ala Pro Gly Gln Gly Leu Glu Trp Ile Gly Leu Ile Asn Pro Gly Ser 
        195                 200                 205             


Gly Gly Thr Asn Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Ile Thr 
    210                 215                 220                 


Ala Asp Lys Ser Thr Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg 
225                 230                 235                 240 


Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Tyr Tyr Gly Asn 
                245                 250                 255     


Ser Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
            260                 265                 270     


<210>  23
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge

<400>  23

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YTHDF2 sgRNA #5

<400>  24
ctactcacgg gccttgcctg                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  YTHDF2 sgRNA #6

<400>  25
ctgcagagac caaaaggtca                                                   20
