                         SEQUENCE LISTING

<110>  Ascendis Pharma Oncology Division A/S
 
<120>  Novel IL-2 sequences and uses thereof

<130>  CPX72963PC

<160>  48    

<170>  PatentIn version 3.5

<210>  1
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A

<400>  1

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Lys Leu Thr 
        35      


<210>  2
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ B

<400>  2

Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys 
1               5                   10                  15      


His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val Leu 
            20                  25                  30          


Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu Ile 
        35                  40                  45              


Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr Thr 
    50                  55                  60                  


Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe Leu 
65                  70                  75                  80  


Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile Ile Ser Thr Leu Thr 
                85                  90                  95  


<210>  3
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34A mutation

<400>  3

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Ala Leu Thr 
        35      


<210>  4
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34C mutation

<400>  4

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Cys Leu Thr 
        35      


<210>  5
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34G mutation

<400>  5

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Gly Leu Thr 
        35      


<210>  6
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34S mutation

<400>  6

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Ser Leu Thr 
        35      


<210>  7
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34T mutation

<400>  7

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Thr Leu Thr 
        35      


<210>  8
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34Q mutation

<400>  8

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Gln Leu Thr 
        35      


<210>  9
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34E mutation

<400>  9

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Glu Leu Thr 
        35      


<210>  10
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34N mutation

<400>  10

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Asn Leu Thr 
        35      


<210>  11
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ A with K34D mutation

<400>  11

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Asp Leu Thr 
        35      


<210>  12
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SEQ B with C87S mutation

<400>  12

Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys 
1               5                   10                  15      


His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro Leu Glu Glu Val Leu 
            20                  25                  30          


Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg Pro Arg Asp Leu Ile 
        35                  40                  45              


Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys Gly Ser Glu Thr Thr 
    50                  55                  60                  


Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr Ile Val Glu Phe Leu 
65                  70                  75                  80  


Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile Ile Ser Thr Leu Thr 
                85                  90                  95  


<210>  13
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:1 and SEQ B = SEQ ID 
       NO:2

<400>  13

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  14
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:1 and SEQ B = SEQ ID 
       NO:12

<400>  14

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  15
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aldesleukin

<400>  15

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys 
        35                  40                  45              


Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro 
    50                  55                  60                  


Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg 
65                  70                  75                  80  


Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys 
                85                  90                  95      


Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr 
            100                 105                 110         


Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile Ile 
        115                 120                 125             


Ser Thr Leu Thr 
    130         


<210>  16
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding for IL-2 of SEQ ID NO:18

<400>  16
cccacttctt cgtcaaccaa aaagacacaa cttcaattgg agcacttatt gctggacttg       60

cagatgatat tgaatggaat taataactac aaaaatccta agcttacttg tatgctgact      120

ttcaaatttt atatgccaaa aaaggcaact gagctaaagc atctgcaatg tttagaagag      180

gaactaaaac cattggaaga ggtattgaat ctcgctcaat ctaaaaactt tcatcttcgt      240

cctagagatt tgatttccaa catcaatgtt atcgtgctgg aattgaaggg tagcgaaaca      300

actttcatgt gcgaatacgc cgatgagaca gctactatcg tcgagttctt aaacagatgg      360

attacatttt cccagagtat tatctcaacg ttgacctaat ga                         402


<210>  17
<211>  405
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding IL-2 of SEQ ID NO:14

<400>  17
gcacctacat cttcttcaac taaaaagact caattacagt tggagcatct cttgcttgat       60

ttgcaaatga ttctgaatgg tatcaacaac tataaaaatc caaagctaac ctgtatgttg      120

acgtttaagt tttacatgcc aaaaaaagca acagagctta agcacttgca atgtttggaa      180

gaggaactga aaccccttga agaggttttg aatctggctc agtccaaaaa cttccattta      240

agacctcgtg acctaatttc caacatcaat gtaatcgtct tggaattgaa gggatcagag      300

acaactttca tgtgcgaata cgctgatgag actgccacaa tagtggaatt tctgaataga      360

tggattacct tctcgcaaag cattatcagt actttaactt aatga                      405


<210>  18
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 sequence of SEQ ID NO:1 + cysteine + SEQ ID NO:12

<400>  18

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Lys Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys 
        35                  40                  45              


Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro 
    50                  55                  60                  


Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg 
65                  70                  75                  80  


Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys 
                85                  90                  95      


Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr 
            100                 105                 110         


Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile Ile 
        115                 120                 125             


Ser Thr Leu Thr 
    130         


<210>  19
<211>  4844
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pCSP1007

<400>  19
gggccccaaa catttgctcc ccctagtctc cagggaaatg taaaatatac tgctaataga       60

aaacagtaag acgctcagtt gtcaggataa ttacgttcga ctgtagtaaa acaggaatct      120

gtattgttag aaagaacgag agttttttac ggcgccgcca tattgggccg tgtgaaaaca      180

gcttgaaacc ccactacttt caaaggttct gttgctatac acgaaccatg tttaaccaac      240

ctcgcttttg acttgactga agtcatcggt taacaatcaa gtaccctagt ctgtctgaat      300

gctcctttcc atattcagta ggtgtttctt gcacttttgc atgcactgcg gaagaattag      360

ccaatagcgc gtttcatatg cgcttttacc ccctcttttg tcaagcgcaa aatgcctgta      420

agatttggtg ggggtgtgag ccgttagctg aagtacaaca ggctaattcc ctgaaaaaac      480

tgcagctcag ggattcccac tatttggtat tctgatatgt ttttcctgat atgcatcaaa      540

actctaatct aaaacctgaa tctccgctat tttttttttt tttgatgacc ccgttttcgt      600

gacaaattaa tttccaacgg ggtcttgtcc ggataagaga attttgtttg attatccgtt      660

cggataaatg gacgcctgct ccatattttt ccggttatta ccccacctgg aagtgcccag      720

aattttccgg ggattacgga taatacggtg gtctggatta attaatacga gatctcaggg      780

attcccacta tttggtattc tgatatgttt ttcctgatat gcatcaaaac tctaatctaa      840

aacctgaatc tccgctattt tttttttttt tgatgacccc gttttcgtga caaattaatt      900

tccaacgggg tcttgtccgg ataagagaat tttgtttgat tatccgttcg gataaatgga      960

cgcctgctcc atatttttcc ggttattacc ccacctggaa gtgcccagaa ttttccgggg     1020

attacggata atacggtggt ctggattaat taatacgcca agtcttacat tttgttgcag     1080

tctcgtgcga gtatgtgcaa taataaacaa gatgagccaa tttattggat tagttgcagc     1140

ttgaccccgc catagctagg catagccaag tgctatgggt gttagatgat gcacttggat     1200

gcagtgagtt ttggagtata aaagatcctt aaaattccac ccttcctgca ggccatgaga     1260

tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc tgctccagtc     1320

aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat cggttactca     1380

gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac aaataacggg     1440

ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg ggtatctctc     1500

gagaaaagac ccacttcttc gtcaaccaaa aagacacaac ttcaattgga gcacttattg     1560

ctggacttgc agatgatatt gaatggaatt aataactaca aaaatcctaa gcttacttgt     1620

atgctgactt tcaaatttta tatgccaaaa aaggcaactg agctaaagca tctgcaatgt     1680

ttagaagagg aactaaaacc attggaagag gtattgaatc tcgctcaatc taaaaacttt     1740

catcttcgtc ctagagattt gatttccaac atcaatgtta tcgtgctgga attgaagggt     1800

agcgaaacaa ctttcatgtg cgaatacgcc gatgagacag ctactatcgt cgagttctta     1860

aacagatgga ttacattttc ccagagtatt atctcaacgt tgacctaata gcggccgcct     1920

cggccggatc cacgtccgac ggcggcccac gggtccgagg cctcggagat ccgtccccct     1980

tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca     2040

catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt     2100

tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc     2160

tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg     2220

gacgctcgaa ggctttaatt tgcaagctac cggtggtacc acacaccata gcttcaaaat     2280

gtttctactc cttttttact cttccagatt ttctcggact ccgcgcatcg ccgtaccact     2340

tcaaaacacc caagcacagc atactaaatt tcccctcttt cttcctctag ggtgtcgtta     2400

attacccgta ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct ttttcttcgt     2460

cgaaaaaggc aataaaaatt tttatcacgt ttctttttct tgaaaatttt tttttttgat     2520

ttttttctct ttcgatgacc tcccattgat atttaagtta ataaacggtc ttcaatttct     2580

caagtttcag tttcattttt cttgttctat tacaactttt tttacttctt gctcattaga     2640

aagaaagcat agcaatctaa tctaagggcg gtgttgacaa ttaatcatcg gcatagtata     2700

tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg     2760

ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg     2820

ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc     2880

tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg     2940

tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg     3000

acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc     3060

tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgatcagtac     3120

ttactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag tttttttata     3180

ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt cgcctcgaca     3240

tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc aatcgtatgt     3300

gaatgctggt cgctatactg gtaccgagct ctgagcaaaa ggccagcaaa gggccaggaa     3360

ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca     3420

caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc     3480

gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata     3540

cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta     3600

tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca     3660

gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga     3720

cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg     3780

tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg     3840

tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg     3900

cagacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     3960

aaaaaaagga tctcaagaag atcctttgat cttttctacg ggagctctta attaagcggc     4020

cgcagcaacg ttgtcactga agttggcatc agtatctaca aaccctacat acctcttgaa     4080

ggtccagaag gtaagcattt gcccgctcca ttcctacccg acatatccac tctacatttt     4140

atagttcaag gcaccgaaaa agttcgaaac aagaagtttg ttcctgataa caaggatttc     4200

tttattggtg ggacttcatt tactgtctcc aagaaggaca tttctgctgt cataaccgag     4260

atagtctcac agttcgagtc tactgatgac caaaagtcag agaaatttac catgaaatct     4320

cctcctccgg ttgctttagt tgggcataat ctgataggag acctcaagac actgaagaat     4380

gcgggtatca ccattcccat acttccaatc ataggatccg gcgcgccgat actcgagaat     4440

tatggcttaa tcaagtgaat acatcaaagt caaacttaaa atacattctt cgcaggcttg     4500

gcttgccaca tagttttctt cacaatgctg caaatgacgc ttattatacc cttttggcat     4560

cgttgaagct tgcacaaacg aacgtcccac ttaatcttct gtactctgaa gaggagtggg     4620

aaataccaag aaaaacatca aactcgaatg attttcccga acccctacca caagatattc     4680

atcagctgcg agataggctg atcaggagca agctcgtacg agaagaaaca aaatgacaaa     4740

aaaaatccta tactatatag gttacaaata aaaaagtatc aaaaatgaag cctgcatctc     4800

tcaggcaaat ggcattctga catcctctgc ggccgcttaa ttaa                      4844


<210>  20
<211>  4847
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pCKP1036

<400>  20
gggccccaaa catttgctcc ccctagtctc cagggaaatg taaaatatac tgctaataga       60

aaacagtaag acgctcagtt gtcaggataa ttacgttcga ctgtagtaaa acaggaatct      120

gtattgttag aaagaacgag agttttttac ggcgccgcca tattgggccg tgtgaaaaca      180

gcttgaaacc ccactacttt caaaggttct gttgctatac acgaaccatg tttaaccaac      240

ctcgcttttg acttgactga agtcatcggt taacaatcaa gtaccctagt ctgtctgaat      300

gctcctttcc atattcagta ggtgtttctt gcacttttgc atgcactgcg gaagaattag      360

ccaatagcgc gtttcatatg cgcttttacc ccctcttttg tcaagcgcaa aatgcctgta      420

agatttggtg ggggtgtgag ccgttagctg aagtacaaca ggctaattcc ctgaaaaaac      480

tgcagctcag ggattcccac tatttggtat tctgatatgt ttttcctgat atgcatcaaa      540

actctaatct aaaacctgaa tctccgctat tttttttttt tttgatgacc ccgttttcgt      600

gacaaattaa tttccaacgg ggtcttgtcc ggataagaga attttgtttg attatccgtt      660

cggataaatg gacgcctgct ccatattttt ccggttatta ccccacctgg aagtgcccag      720

aattttccgg ggattacgga taatacggtg gtctggatta attaatacga gatctcaggg      780

attcccacta tttggtattc tgatatgttt ttcctgatat gcatcaaaac tctaatctaa      840

aacctgaatc tccgctattt tttttttttt tgatgacccc gttttcgtga caaattaatt      900

tccaacgggg tcttgtccgg ataagagaat tttgtttgat tatccgttcg gataaatgga      960

cgcctgctcc atatttttcc ggttattacc ccacctggaa gtgcccagaa ttttccgggg     1020

attacggata atacggtggt ctggattaat taatacgcca agtcttacat tttgttgcag     1080

tctcgtgcga gtatgtgcaa taataaacaa gatgagccaa tttattggat tagttgcagc     1140

ttgaccccgc catagctagg catagccaag tgctatgggt gttagatgat gcacttggat     1200

gcagtgagtt ttggagtata aaagatcctt aaaattccac ccttcctgca ggccatgaga     1260

tttccttcaa tttttactgc tgttttattc gcagcatcct ccgcattagc tgctccagtc     1320

aacactacaa cagaagatga aacggcacaa attccggctg aagctgtcat cggttactca     1380

gatttagaag gggatttcga tgttgctgtt ttgccatttt ccaacagcac aaataacggg     1440

ttattgttta taaatactac tattgccagc attgctgcta aagaagaagg ggtatctctc     1500

gagaaaagag cacctacatc ttcttcaact aaaaagactc aattacagtt ggagcatctc     1560

ttgcttgatt tgcaaatgat tctgaatggt atcaacaact ataaaaatcc aaagctaacc     1620

tgtatgttga cgtttaagtt ttacatgcca aaaaaagcaa cagagcttaa gcacttgcaa     1680

tgtttggaag aggaactgaa accccttgaa gaggttttga atctggctca gtccaaaaac     1740

ttccatttaa gacctcgtga cctaatttcc aacatcaatg taatcgtctt ggaattgaag     1800

ggatcagaga caactttcat gtgcgaatac gctgatgaga ctgccacaat agtggaattt     1860

ctgaatagat ggattacctt ctcgcaaagc attatcagta ctttaactta atagcggccg     1920

cctcggccgg atccacgtcc gacggcggcc cacgggtccg aggcctcgga gatccgtccc     1980

ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcccc     2040

ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt     2100

atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt     2160

ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt     2220

tgggacgctc gaaggcttta atttgcaagc taccggtggt accacacacc atagcttcaa     2280

aatgtttcta ctcctttttt actcttccag attttctcgg actccgcgca tcgccgtacc     2340

acttcaaaac acccaagcac agcatactaa atttcccctc tttcttcctc tagggtgtcg     2400

ttaattaccc gtactaaagg tttggaaaag aaaaaagaga ccgcctcgtt tctttttctt     2460

cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt tcttgaaaat tttttttttt     2520

gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg gtcttcaatt     2580

tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt cttgctcatt     2640

agaaagaaag catagcaatc taatctaagg gcggtgttga caattaatca tcggcatagt     2700

atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg     2760

ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc     2820

tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga     2880

ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt     2940

gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc     3000

gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg     3060

ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgatcag     3120

tacttactga caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt     3180

atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg     3240

acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta     3300

tgtgaatgct ggtcgctata ctggtaccga gctctgagca aaaggccagc aaagggccag     3360

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     3420

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     3480

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     3540

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     3600

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     3660

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     3720

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     3780

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt     3840

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     3900

cggcagacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     3960

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acgggagctc ttaattaagc     4020

ggccgcagca acgttgtcac tgaagttggc atcagtatct acaaacccta catacctctt     4080

gaaggtccag aaggtaagca tttgcccgct ccattcctac ccgacatatc cactctacat     4140

tttatagttc aaggcaccga aaaagttcga aacaagaagt ttgttcctga taacaaggat     4200

ttctttattg gtgggacttc atttactgtc tccaagaagg acatttctgc tgtcataacc     4260

gagatagtct cacagttcga gtctactgat gaccaaaagt cagagaaatt taccatgaaa     4320

tctcctcctc cggttgcttt agttgggcat aatctgatag gagacctcaa gacactgaag     4380

aatgcgggta tcaccattcc catacttcca atcataggat ccggcgcgcc gatactcgag     4440

aattatggct taatcaagtg aatacatcaa agtcaaactt aaaatacatt cttcgcaggc     4500

ttggcttgcc acatagtttt cttcacaatg ctgcaaatga cgcttattat acccttttgg     4560

catcgttgaa gcttgcacaa acgaacgtcc cacttaatct tctgtactct gaagaggagt     4620

gggaaatacc aagaaaaaca tcaaactcga atgattttcc cgaaccccta ccacaagata     4680

ttcatcagct gcgagatagg ctgatcagga gcaagctcgt acgagaagaa acaaaatgac     4740

aaaaaaaatc ctatactata taggttacaa ataaaaaagt atcaaaaatg aagcctgcat     4800

ctctcaggca aatggcattc tgacatcctc tgcggccgct taattaa                   4847


<210>  21
<211>  132
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 mutein

<400>  21

Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His Leu 
1               5                   10                  15      


Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys Asn 
            20                  25                  30          


Pro Lys Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys Lys 
        35                  40                  45              


Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys Pro 
    50                  55                  60                  


Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu Arg 
65                  70                  75                  80  


Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu Lys 
                85                  90                  95      


Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala Thr 
            100                 105                 110         


Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile Ile 
        115                 120                 125             


Ser Thr Leu Thr 
    130         


<210>  22
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>   IL-2 of formula (I) with SEQ A = SEQ ID NO:3 and SEQ B = SEQ ID 
       NO:2

<400>  22

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Ala Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  23
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:3 and SEQ B = SEQ ID 
       NO:12

<400>  23

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Ala Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  24
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:4 and SEQ B = SEQ ID 
       NO:2

<400>  24

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Cys Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  25
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:4 and SEQ B = SEQ ID 
       NO:12

<400>  25

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Cys Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  26
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:5 and SEQ B = SEQ ID 
       NO:2

<400>  26

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Gly Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  27
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:5 and SEQ B = SEQ ID 
       NO:12

<400>  27

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Gly Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  28
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:6 and SEQ B = SEQ ID 
       NO:2

<400>  28

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Ser Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  29
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:6 and SEQ B = SEQ ID 
       NO:12

<400>  29

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Ser Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  30
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:7 and SEQ B = SEQ ID 
       NO:2

<400>  30

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Thr Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  31
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:7 and SEQ B = SEQ ID 
       NO:12

<400>  31

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Thr Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  32
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:8 and SEQ B = SEQ ID 
       NO:2

<400>  32

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Gln Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  33
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:8 and SEQ B = SEQ ID 
       NO:12

<400>  33

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Gln Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  34
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:9 and SEQ B = SEQ ID 
       NO:2

<400>  34

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Glu Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  35
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:9 and SEQ B = SEQ ID 
       NO:12

<400>  35

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Glu Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  36
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:10 and SEQ B = SEQ ID 
       NO:2

<400>  36

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Asn Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  37
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:10 and SEQ B = SEQ ID 
       NO:12

<400>  37

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Asn Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  38
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:11 and SEQ B = SEQ ID 
       NO:2

<400>  38

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Asp Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Cys Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  39
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 of formula (I) with SEQ A = SEQ ID NO:11 and SEQ B = SEQ ID 
       NO:12

<400>  39

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Asp Leu Thr Cys Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr 
    130             


<210>  40
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding SEQ ID NO:14 in E.Coli

<400>  40
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt acttctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac ctgtatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttctcaaag catcatctca acactgactt aa                         402


<210>  41
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding SEQ ID NO:35 in E.Coli

<400>  41
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt acttctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccgaactcac ctgtatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttctcaaag catcatctca acactgactt aa                         402


<210>  42
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding SEQ ID NO:39 in E.Coli

<400>  42
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt acttctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccgacctcac ctgtatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttctcaaag catcatctca acactgactt aa                         402


<210>  43
<211>  402
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding SEQ ID NO:33 in E.Coli

<400>  43
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt acttctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc cccaactcac ctgtatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttctcaaag catcatctca acactgactt aa                         402


<210>  44
<211>  401
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence encoding SEQ ID NO:29 in E.Coli

<400>  44
gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt acttctggat       60

ttacagatga ttttgaatgg aattaataat tacaagaatc ccagcctcac ctgtatgctc      120

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      180

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      240

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      300

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      360

tggattacct tttctcaaag catcatctca acactgactt a                          401


<210>  45
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 variant

<400>  45

Met Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu 
1               5                   10                  15      


His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr 
            20                  25                  30          


Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro 
        35                  40                  45              


Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu 
    50                  55                  60                  


Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His 
65                  70                  75                  80  


Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu 
                85                  90                  95      


Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr 
            100                 105                 110         


Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser 
        115                 120                 125             


Ile Ile Ser Thr Leu Thr 
    130                 


<210>  46
<211>  139
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2 variant with His-tag

<400>  46

Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      


Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          


Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              


Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  


Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  


Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      


Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         


Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ser Gln Ser Ile 
        115                 120                 125             


Ile Ser Thr Leu Thr His His His His His His 
    130                 135                 


<210>  47
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide S1

<400>  47

Met Glu Phe Gly Leu Ser Trp Leu Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys 
          

<210>  48
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide S2

<400>  48

Met Pro Leu Leu Leu Leu Leu Pro Leu Leu Trp Ala Gly Ala Leu Ala 
1               5                   10                  15      


