                         SEQUENCE LISTING

<110>  MV BioTherapeutics SA
 
<120>  COMBINATION OF AN ATP-HYDROLYZING ENZYME AND AN IMMUNE CHECKPOINT
        MODULATOR AND USES THEREOF

<130>  MV03P002WO1

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  246
<212>  PRT
<213>  Shigella flexneri

<400>  1

Met Lys Thr Lys Asn Phe Leu Leu Phe Cys Ile Ala Thr Asn Met Ile 
1               5                   10                  15      


Phe Ile Pro Ser Ala Asn Ala Leu Lys Ala Glu Gly Phe Leu Thr Gln 
            20                  25                  30          


Gln Thr Ser Pro Asp Ser Leu Ser Ile Leu Pro Pro Pro Pro Ala Glu 
        35                  40                  45              


Asp Ser Val Val Phe Leu Ala Asp Lys Ala His Tyr Glu Phe Gly Arg 
    50                  55                  60                  


Ser Leu Arg Asp Ala Asn Arg Val Arg Leu Ala Ser Glu Asp Ala Tyr 
65                  70                  75                  80  


Tyr Glu Asn Phe Gly Leu Ala Phe Ser Asp Ala Tyr Gly Met Asp Ile 
                85                  90                  95      


Ser Arg Glu Asn Thr Pro Ile Leu Tyr Gln Leu Leu Thr Gln Val Leu 
            100                 105                 110         


Gln Asp Ser His Asp Tyr Ala Val Arg Asn Ala Lys Glu Tyr Tyr Lys 
        115                 120                 125             


Arg Val Arg Pro Phe Val Ile Tyr Lys Asp Ala Thr Cys Thr Pro Asp 
    130                 135                 140                 


Lys Asp Glu Lys Met Ala Ile Thr Gly Ser Tyr Pro Ser Gly His Ala 
145                 150                 155                 160 


Ser Phe Gly Trp Ala Val Ala Leu Ile Leu Ala Glu Ile Asn Pro Gln 
                165                 170                 175     


Arg Lys Ala Glu Ile Leu Arg Arg Gly Tyr Glu Phe Gly Glu Ser Arg 
            180                 185                 190         


Val Ile Cys Gly Ala His Trp Gln Ser Asp Val Glu Ala Gly Arg Leu 
        195                 200                 205             


Met Gly Ala Ser Val Val Ala Val Leu His Asn Thr Pro Glu Phe Thr 
    210                 215                 220                 


Lys Ser Leu Ser Glu Ala Lys Lys Glu Phe Glu Glu Leu Asn Thr Pro 
225                 230                 235                 240 


Thr Asn Glu Leu Thr Pro 
                245     


<210>  2
<211>  246
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Loss-of-function isoform of apyrase

<400>  2

Met Lys Thr Lys Asn Phe Leu Leu Phe Cys Ile Ala Thr Asn Met Ile 
1               5                   10                  15      


Phe Ile Pro Ser Ala Asn Ala Leu Lys Ala Glu Gly Phe Leu Thr Gln 
            20                  25                  30          


Gln Thr Ser Pro Asp Ser Leu Ser Ile Leu Pro Pro Pro Pro Ala Glu 
        35                  40                  45              


Asp Ser Val Val Phe Leu Ala Asp Lys Ala His Tyr Glu Phe Gly Arg 
    50                  55                  60                  


Ser Leu Arg Asp Ala Asn Arg Val Arg Leu Ala Ser Glu Asp Ala Tyr 
65                  70                  75                  80  


Tyr Glu Asn Phe Gly Leu Ala Phe Ser Asp Ala Tyr Gly Met Asp Ile 
                85                  90                  95      


Ser Arg Glu Asn Thr Pro Ile Leu Tyr Gln Leu Leu Thr Gln Val Leu 
            100                 105                 110         


Gln Asp Ser His Asp Tyr Ala Val Arg Asn Ala Lys Glu Tyr Tyr Lys 
        115                 120                 125             


Arg Val Arg Pro Phe Val Ile Tyr Lys Asp Ala Thr Cys Thr Pro Asp 
    130                 135                 140                 


Lys Asp Glu Lys Met Ala Ile Thr Gly Ser Tyr Pro Ser Gly His Ala 
145                 150                 155                 160 


Ser Phe Gly Trp Ala Val Ala Leu Ile Leu Ala Glu Ile Asn Pro Gln 
                165                 170                 175     


Arg Lys Ala Glu Ile Leu Arg Arg Gly Tyr Glu Phe Gly Glu Ser Pro 
            180                 185                 190         


Val Ile Cys Gly Ala His Trp Gln Ser Asp Val Glu Ala Gly Arg Leu 
        195                 200                 205             


Met Gly Ala Ser Val Val Ala Val Leu His Asn Thr Pro Glu Phe Thr 
    210                 215                 220                 


Lys Ser Leu Ser Glu Ala Lys Lys Glu Phe Glu Glu Leu Asn Thr Pro 
225                 230                 235                 240 


Thr Asn Glu Leu Thr Pro 
                245     


<210>  3
<211>  741
<212>  DNA
<213>  Shigella flexneri

<400>  3
atgaaaacca aaaactttct tcttttttgt attgctacaa atatgatttt tatcccctca       60

gcaaatgctc tgaaggcaga aggttttctc actcaacaaa cttcaccaga cagtttgtca      120

atacttccgc cgcctccggc agaggattca gtagtatttc tggctgacaa agctcattat      180

gaattcggcc gctcgctccg ggatgctaat cgtgtacgtc tcgctagcga agatgcatac      240

tacgagaatt ttggtcttgc attttcagat gcttatggca tggatatttc aagggaaaat      300

accccaatct tatatcagtt gttaacacaa gtactacagg atagccatga ttacgccgtg      360

cgtaacgcca aagaatatta taaaagagtt cgtccattcg ttatttataa agacgcaacc      420

tgtacacctg ataaagatga gaaaatggct atcactggct cttatccctc tggtcatgca      480

tcctttggtt gggcagtagc actgatactt gcggagatta atcctcaacg taaagcggaa      540

atacttcgac gtggatatga gtttggagaa agtcgggtca tctgcggtgc gcattggcaa      600

agcgatgtag aggctgggcg tttaatggga gcatcggttg ttgcagtact tcataataca      660

cctgaattta ccaaaagcct tagcgaagcc aaaaaagagt ttgaagaatt aaatactcct      720

accaatgaac tgaccccata a                                                741


<210>  4
<211>  840
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EcN malP gene portion

<400>  4
cgagcaggca cactggaagt attgctgcat caggcgcagc tttttaccgg cagtatggtt       60

gtcgtttgga tagagaactt tggtcagttt ttccgcgttg atgccctgct gttcggcacg      120

caggaaatca ccgtcgttaa atttagtcag atcaaacgga tgcgcatgcg tcgcctgcca      180

cagacgcagt ggctgcgcca cgccattacg atagccgaca acggggagat cccacgcttg      240

accggtaatg gtaaactccg gctcccagcg tccatctttc gtcactttac cgccaatccc      300

tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttaccgc gatgccagtc      360

atccggcgct tcaacctgtt tgccatcgac aaatgactgg cggaacaagc catattgata      420

attaaggccg tagccagtag ctgactgccc gacagttgcc attgagtcga ggaagcacgc      480

cgccagacgt cccagaccac cgttccccag cgccgggtcg atctcttctt ccaacaggtc      540

agtcaggttg atgtcataag ccttcaacga atcctgtaca tcctgatacc agccgagatt      600

caacaggttg ttgcccgtca ggcgaccaat caaaaactcc attgagatgt agttaacatg      660

tcgctgattc gccactggct tggcgaatgg ctgagcacgc agcatttcgg ccagtgcttc      720

gctcactgcc agccaccact ggcgaggagt catttcagcc gcagaattta agccataacg      780

ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag gttgtgacat      840


<210>  5
<211>  813
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  EcN malT gene portion

<400>  5
atgctgattc cgtcaaaatt aagtcgtccg gttcgactcg accataccgt ggttcgtgag       60

cgcctgctgg ctaaactttc cggcgcgaac aacttccggc tggcgctgat cacaagtcct      120

gcgggctacg gaaagaccac gctcatttcc cagtgggcgg caggcaaaaa cgatatcggc      180

tggtactcgc tggatgaagg tgataaccag caagagcgtt tcgccagcta tctcattgcc      240

gccgtgcaac aggcaaccaa cggtcactgc gcgatatgtg agacgatggc gcaaaaacgg      300

caatatgcca gcctgacgtc actcttcgcc cagcttttca ttgagctggc ggaatggcat      360

agcccacttt atctggtcat cgatgactat catctgatca ctaatcctgt gatccacgag      420

tcaatgcgct tctttattcg ccatcaacca gaaaatctca cccttgtggt gttgtcacgc      480

aaccttccgc aactgggcat tgccaatctg cgtgttcgtc cagctagcga attcgctgga      540

aattggcagt cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg      600

tctgtcatcg ccgattgaag ctgcagaaag cagtcggatt tgtgatgatg tttccggttg      660

ggcgacggca ctgcagctaa tcgccctctc cgcccggcag aatactcact cagcccataa      720

gtcggcacgc cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga      780

ggttttggat aacgtcgatc tcgcaacgcg cca                                   813


<210>  6
<211>  1285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment including the PproD promoter, the BBa_BB0032 RBS, 
       the S. flexneri phoN2 gene and the phoN2 transcriptional 
       terminator

<400>  6
cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac       60

tttacgggca tgcataaggc tcgtataata tattcaggga gaccacaacg gtttccctct      120

acaaataatt ttgtttaact tttactagag tcacacagga aagtactaga tgaaaaccaa      180

aaactttctt cttttttgta ttgctacaaa tatgattttt atcccctcag caaatgctct      240

gaaggcagaa ggttttctca ctcaacaaac ttcaccagac agtttgtcaa tacttccgcc      300

gcctccggca gaggattcag tagtatttct ggctgacaaa gctcattatg aattcggccg      360

ctcgctccgg gatgctaatc gtgtacgtct cgctagcgaa gatgcatact acgagaattt      420

tggtcttgca ttttcagatg cttatggcat ggatatttca agggaaaata ccccaatctt      480

atatcagttg ttaacacaag tactacagga tagccatgat tacgccgtgc gtaacgccaa      540

agaatattat aaaagagttc gtccattcgt tatttataaa gacgcaacct gtacacctga      600

taaagatgag aaaatggcta tcactggctc ttatccctct ggtcatgcat cctttggttg      660

ggcagtagca ctgatacttg cggagattaa tcctcaacgt aaagcggaaa tacttcgacg      720

tggatatgag tttggagaaa gtcgggtcat ctgcggtgcg cattggcaaa gcgatgtaga      780

ggctgggcgt ttaatgggag catcggttgt tgcagtactt cataatacac ctgaatttac      840

caaaagcctt agcgaagcca aaaaagagtt tgaagaatta aatactccta ccaatgaact      900

gaccccataa agctggacag cctgtatcag gctatggagg gcccatagac aaatctaccc      960

tatatgagca taggaggagt ctatgggcac accacgtttt acccctgaat ttaagggatt     1020

actggaaagg ctgggacata tccctccggc agaagcagaa aaagcttatt atgctgccat     1080

cggaaacgat gatctggcaa cctgagttca cagataaaac attctctagg aaactcgggg     1140

cggttccgtt caccacatgc aatgtggtgt tgcaggggaa cggtctgccc atcccctatg     1200

tcgatcaata taacagaaat gacaacttca gattcagggc acaacctaaa tatattttag     1260

gtcacctctc aaatcgtttg cctga                                           1285


<210>  7
<211>  995
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA fragment including the E. coli cat gene flanked by the FRT 
       sequences

<400>  7
gaagttccta ttctctagaa agtataggaa cttcggcgcg cctacctgtg acggaagatc       60

acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact      120

tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa      180

taagatcact accgggcgta ttttttgagt tgtcgagatt ttcaggagct aaggaagcta      240

aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag      300

aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg      360

atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta      420

ttcacattct tgcccgcctg atgaatgctc atccggaatt acgtatggca atgaaagacg      480

gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg      540

aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat      600

attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg      660

agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg      720

tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag      780

gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc      840

atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt      900

aaggcgcgcc atttaaatga agttcctatt ccgaagttcc tattctctag aaagtatagg      960

aacttcgaag cagctccagc ctacacaatg aattc                                 995


<210>  8
<211>  1158
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA encoding chicken ovalbumin

<400>  8
atgggctcca tcggtgcagc aagcatggaa ttttgttttg atgtattcaa ggagctcaaa       60

gtccaccatg ccaatgagaa catcttctac tgccccattg ccatcatgtc agctctagcc      120

atggtatacc tgggtgcaaa agacagcacc aggacacaaa taaataaggt tgttcgcttt      180

gataaacttc caggattcgg agacagtatt gaagctcagt gtggcacatc tgtaaacgtt      240

cactcttcac ttagagacat cctcaaccaa atcaccaaac caaatgatgt ttattcgttc      300

agccttgcca gtagacttta tgctgaagag agatacccaa tcctgccaga atacttgcag      360

tgtgtgaagg aactgtatag aggaggcttg gaacctatca actttcaaac agctgcagat      420

caagccagag agctcatcaa ttcctgggta gaaagtcaga caaatggaat tatcagaaat      480

gtccttcagc caagctccgt ggattctcaa actgcaatgg ttctggttaa tgccattgtc      540

ttcaaaggac tgtgggagaa agcatttaag gatgaagaca cacaagcaat gcctttcaga      600

gtgactgagc aagaaagcaa acctgtgcag atgatgtacc agattggttt atttagagtg      660

gcatcaatgg cttctgagaa aatgaagatc ctggagcttc catttgccag tgggacaatg      720

agcatgttgg tgctgttgcc tgatgaagtc tcaggccttg agcagcttga gagtataatc      780

aactttgaaa aactgactga atggaccagt tctaatgtta tggaagagag gaagatcaaa      840

gtgtacttac ctcgcatgaa gatggaggaa aaatacaacc tcacatctgt cttaatggct      900

atgggcatta ctgacgtgtt tagctcttca gccaatctgt ctggcatctc ctcagcagag      960

agcctgaaga tatctcaagc tgtccatgca gcacatgcag aaatcaatga agcaggcaga     1020

gaggtggtag ggtcagcaga ggctggagtg gatgctgcaa gcgtctctga agaatttagg     1080

gctgaccatc cattcctctt ctgtatcaag cacatcgcaa ccaacgccgt tctcttcttt     1140

ggcagatgtg tttcccct                                                   1158


<210>  9
<211>  386
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  chicken ovalbumin

<400>  9

Met Gly Ser Ile Gly Ala Ala Ser Met Glu Phe Cys Phe Asp Val Phe 
1               5                   10                  15      


Lys Glu Leu Lys Val His His Ala Asn Glu Asn Ile Phe Tyr Cys Pro 
            20                  25                  30          


Ile Ala Ile Met Ser Ala Leu Ala Met Val Tyr Leu Gly Ala Lys Asp 
        35                  40                  45              


Ser Thr Arg Thr Gln Ile Asn Lys Val Val Arg Phe Asp Lys Leu Pro 
    50                  55                  60                  


Gly Phe Gly Asp Ser Ile Glu Ala Gln Cys Gly Thr Ser Val Asn Val 
65                  70                  75                  80  


His Ser Ser Leu Arg Asp Ile Leu Asn Gln Ile Thr Lys Pro Asn Asp 
                85                  90                  95      


Val Tyr Ser Phe Ser Leu Ala Ser Arg Leu Tyr Ala Glu Glu Arg Tyr 
            100                 105                 110         


Pro Ile Leu Pro Glu Tyr Leu Gln Cys Val Lys Glu Leu Tyr Arg Gly 
        115                 120                 125             


Gly Leu Glu Pro Ile Asn Phe Gln Thr Ala Ala Asp Gln Ala Arg Glu 
    130                 135                 140                 


Leu Ile Asn Ser Trp Val Glu Ser Gln Thr Asn Gly Ile Ile Arg Asn 
145                 150                 155                 160 


Val Leu Gln Pro Ser Ser Val Asp Ser Gln Thr Ala Met Val Leu Val 
                165                 170                 175     


Asn Ala Ile Val Phe Lys Gly Leu Trp Glu Lys Ala Phe Lys Asp Glu 
            180                 185                 190         


Asp Thr Gln Ala Met Pro Phe Arg Val Thr Glu Gln Glu Ser Lys Pro 
        195                 200                 205             


Val Gln Met Met Tyr Gln Ile Gly Leu Phe Arg Val Ala Ser Met Ala 
    210                 215                 220                 


Ser Glu Lys Met Lys Ile Leu Glu Leu Pro Phe Ala Ser Gly Thr Met 
225                 230                 235                 240 


Ser Met Leu Val Leu Leu Pro Asp Glu Val Ser Gly Leu Glu Gln Leu 
                245                 250                 255     


Glu Ser Ile Ile Asn Phe Glu Lys Leu Thr Glu Trp Thr Ser Ser Asn 
            260                 265                 270         


Val Met Glu Glu Arg Lys Ile Lys Val Tyr Leu Pro Arg Met Lys Met 
        275                 280                 285             


Glu Glu Lys Tyr Asn Leu Thr Ser Val Leu Met Ala Met Gly Ile Thr 
    290                 295                 300                 


Asp Val Phe Ser Ser Ser Ala Asn Leu Ser Gly Ile Ser Ser Ala Glu 
305                 310                 315                 320 


Ser Leu Lys Ile Ser Gln Ala Val His Ala Ala His Ala Glu Ile Asn 
                325                 330                 335     


Glu Ala Gly Arg Glu Val Val Gly Ser Ala Glu Ala Gly Val Asp Ala 
            340                 345                 350         


Ala Ser Val Ser Glu Glu Phe Arg Ala Asp His Pro Phe Leu Phe Cys 
        355                 360                 365             


Ile Lys His Ile Ala Thr Asn Ala Val Leu Phe Phe Gly Arg Cys Val 
    370                 375                 380                 


Ser Pro 
385     


