                         SEQUENCE LISTING

<110>  ϴѧ
 
<120>  ѿǵѿǵøͻ

<150>  CN202010507573.9
<151>  2020-06-05

<160>  38    

<170>  PatentIn version 3.3

<210>  1
<211>  572
<212>  PRT
<213>  Thermobifida fusca

<400>  1

Ala Pro Ser Gly Asn Arg Asp Val Ile Val His Leu Phe Gln Trp Arg 
1               5                   10                  15      


Trp Lys Ser Ile Ala Asp Glu Cys Arg Thr Thr Leu Gly Pro His Gly 
            20                  25                  30          


Phe Gly Ala Val Gln Val Ser Pro Pro Gln Glu His Val Val Leu Pro 
        35                  40                  45              


Ala Glu Asp Tyr Pro Trp Trp Gln Asp Tyr Gln Pro Val Ser Tyr Lys 
    50                  55                  60                  


Leu Asp Gln Thr Arg Arg Gly Ser Arg Ala Asp Phe Ile Asp Met Val 
65                  70                  75                  80  


Asn Thr Cys Arg Glu Ala Gly Val Lys Ile Tyr Val Asp Ala Val Ile 
                85                  90                  95      


Asn His Met Thr Gly Thr Gly Ser Ala Gly Ala Gly Pro Gly Ser Ala 
            100                 105                 110         


Gly Ser Ser Tyr Ser Lys Tyr Asp Tyr Pro Gly Ile Tyr Gln Ser Gln 
        115                 120                 125             


Asp Phe Asn Asp Cys Arg Arg Asp Ile Thr Asn Trp Asn Asp Lys Trp 
    130                 135                 140                 


Glu Val Gln His Cys Glu Leu Val Gly Leu Ala Asp Leu Lys Thr Ser 
145                 150                 155                 160 


Ser Pro Tyr Val Gln Asp Arg Ile Ala Ala Tyr Leu Asn Glu Leu Ile 
                165                 170                 175     


Asp Leu Gly Val Ala Gly Phe Arg Ile Asp Ala Ala Lys His Ile Pro 
            180                 185                 190         


Glu Gly Asp Leu Gln Ala Ile Leu Ser Arg Leu Lys Asn Val His Pro 
        195                 200                 205             


Ala Trp Gly Gly Gly Lys Pro Tyr Ile Phe Gln Glu Val Ile Ala Asp 
    210                 215                 220                 


Ser Thr Ile Ser Thr Gly Ser Tyr Thr His Leu Gly Ser Val Thr Glu 
225                 230                 235                 240 


Phe Gln Tyr His Arg Asp Ile Ser His Ala Phe Ala Asn Gly Asn Ile 
                245                 250                 255     


Ala His Leu Thr Gly Leu Gly Ser Gly Leu Thr Pro Ser Asp Lys Ala 
            260                 265                 270         


Val Val Phe Val Val Asn His Asp Thr Gln Arg Tyr Glu Pro Ile Leu 
        275                 280                 285             


Thr His Thr Asp Gly Ala Arg Tyr Asp Leu Ala Gln Lys Phe Met Leu 
    290                 295                 300                 


Ala His Pro Tyr Gly Thr Pro Lys Val Met Ser Ser Tyr Thr Trp Ser 
305                 310                 315                 320 


Gly Asp Asp Lys Ala Gly Pro Pro Met His Ser Asp Gly Thr Thr Arg 
                325                 330                 335     


Pro Thr Asp Cys Ser Ala Asp Arg Trp Leu Cys Glu His Arg Ala Val 
            340                 345                 350         


Ala Gly Met Val Gly Phe His Asn Ala Val Ala Gly Gln Gly Ile Gly 
        355                 360                 365             


Ser Ala Val Thr Asp Gly Asn Gly Arg Leu Ala Phe Ala Arg Gly Ser 
    370                 375                 380                 


Ala Gly Tyr Ala Ala Phe Asn Ala Thr Asn Thr Ala Trp Thr Arg Thr 
385                 390                 395                 400 


Phe Thr Thr Ser Leu Pro Asp Gly Val Tyr Cys Asp Val Ala Asn Gly 
                405                 410                 415     


Thr Phe Val Asp Gly Val Cys Asp Gly Pro Ser Tyr Gln Val Ser Gly 
            420                 425                 430         


Gly Lys Phe Thr Ala Thr Val Pro Ala Asn Gly Ala Val Ala Leu His 
        435                 440                 445             


Val Glu Ala Pro Gly Ser Cys Gly Pro Asp Gly Cys Gly Thr Pro Pro 
    450                 455                 460                 


Gly Gly Gly Asp Asp Cys Thr Thr Val Thr Ala Arg Phe His Ala Thr 
465                 470                 475                 480 


Val Thr Thr Trp Tyr Gly Gln Glu Val Ala Val Val Gly Ser Ile Pro 
                485                 490                 495     


Glu Leu Gly Ser Trp Gln Pro Ala Gln Gly Val Arg Leu Arg Thr Asp 
            500                 505                 510         


Ser Gly Thr Tyr Pro Val Trp Ser Gly Ala Val Asp Leu Pro Ala Gly 
        515                 520                 525             


Val Gly Phe Glu Tyr Lys Tyr Val Lys Leu Lys Pro Asp Gly Thr Val 
    530                 535                 540                 


Glu Trp Glu Gln Gly Gly Asn Arg Ile Ala Thr Val Asp Asp Ser Gly 
545                 550                 555                 560 


Gly Gly Cys Ser Gln Asn Phe Tyr Asp Ser Trp Arg 
                565                 570         


<210>  2
<211>  1716
<212>  DNA
<213>  Thermobifida fusca

<400>  2
gccccgagtg gtaatcgcga tgttattgtt catctgtttc agtggcgctg gaaaagcatt     60

gcagatgaat gtcgtaccac cttaggtccg catggttttg gtgcagttca ggtgtctccg    120

ccgcaggaac atgttgtgct gccggccgaa gattatccgt ggtggcagga ttatcagccg    180

gtgagctata aattagatca gacccgtcgc ggctcacgcg cagattttat tgatatggtt    240

aatacctgtc gcgaagcagg tgttaaaatt tatgtggatg ccgttattaa tcacatgacc    300

ggaaccggct cagcaggcgc aggtcctggc tcagccggct caagctatag taaatatgat    360

tatccgggca tttatcagtc acaggatttt aatgattgtc gtcgcgatat taccaattgg    420

aatgataaat gggaagttca gcattgtgaa ttagtgggtt tagcagacct gaaaacctct    480

agtccgtatg ttcaggatcg cattgccgcc tatctgaatg aactgattga tctgggcgtt    540

gcaggctttc gtattgatgc cgccaaacat attccggaag gcgatttaca ggccattctg    600

tctcgtctga aaaatgttca tccggcctgg ggcggcggta aaccgtatat ttttcaggaa    660

gttattgcag attctaccat ttcaaccggc tcttataccc atctgggtag cgtgaccgaa    720

tttcagtatc atcgcgatat tagtcatgcc tttgccaatg gcaatattgc acatctgacc    780

ggcttaggta gcggtctgac cccgtcagat aaagcagttg tgtttgttgt taatcatgat    840

acccagcgct atgaaccgat tctgacccat accgatggtg cacgctatga tttagcacag    900

aaatttatgc tggcccatcc gtatggaaca ccgaaagtta tgtcgtctta tacctggagt    960

ggcgatgata aagcaggtcc gccgatgcat agcgatggca ccacccgccc gaccgattgt   1020

agtgcagatc gctggctgtg tgaacatcgc gcagttgccg gcatggtggg ttttcataat   1080

gccgttgcag gccagggcat tggtagtgca gtgaccgatg gtaatggtcg cttagccttt   1140

gcacgcggta gcgcaggcta tgcagccttt aatgcaacca ataccgcctg gacccgcacc   1200

tttaccacct cactgccgga tggtgtgtat tgtgatgttg ccaatggcac ctttgtggat   1260

ggcgtgtgtg atggtccgag ctatcaggtg agcggcggca aatttaccgc aaccgttccg   1320

gccaatggtg ccgttgcctt acatgttgaa gcgcctggtt cttgtggtcc ggatggctgt   1380

ggcaccccgc cgggcggtgg cgatgattgt accaccgtga ccgcacgctt tcatgcaacc   1440

gtgaccacct ggtatggcca ggaagttgcc gttgtgggct caattccgga actgggttct   1500

tggcagccgg cccagggcgt gcgcttacgt accgatagcg gtacatatcc ggtttggagc   1560

ggtgcagtgg atctgccggc aggcgtgggt tttgaatata aatatgttaa actgaaaccg   1620

gatggcaccg ttgaatggga acagggcggt aatcgtattg caaccgtgga tgatagcggc   1680

ggtggctgta gtcagaattt ttatgattct tggcgt                             1716


<210>  3
<211>  1350
<212>  DNA
<213>  ˹

<400>  3
gccccgagtg gtaatcgcga tgttattgtt catctgtttc agtggcgctg gaaaagcatt     60

gcagatgaat gtcgtaccac cttaggtccg catggttttg gtgcagttca ggtgtctccg    120

ccgcaggaac atgttgtgct gccggccgaa gattatccgt ggtggcagga ttatcagccg    180

gtgagctata aattagatca gacccgtcgc ggctcacgcg cagattttat tgatatggtt    240

aatacctgtc gcgaagcagg tgttaaaatt tatgtggatg ccgttattaa tcacatgacc    300

ggaaccggct cagcaggcgc aggtcctggc tcagccggct caagctatag taaatatgat    360

tatccgggca tttatcagtc acaggatttt aatgattgtc gtcgcgatat taccaattgg    420

aatgataaat gggaagttca gcattgtgaa ttagtgggtt tagcagacct gaaaacctct    480

agtccgtatg ttcaggatcg cattgccgcc tatctgaatg aactgattga tctgggcgtt    540

gcaggctttc gtattgatgc cgccaaacat attccggaag gcgatttaca ggccattctg    600

tctcgtctga aaaatgttca tccggcctgg ggcggcggta aaccgtatat ttttcaggaa    660

gttattgcag attctaccat ttcaaccggc tcttataccc atctgggtag cgtgaccgaa    720

tttcagtatc atcgcgatat tagtcatgcc tttgccaatg gcaatattgc acatctgacc    780

ggcttaggta gcggtctgac cccgtcagat aaagcagttg tgtttgttgt taatcatgat    840

acccagcgct atgaaccgat tctgacccat accgatggtg cacgctatga tttagcacag    900

aaatttatgc tggcccatcc gtatggaaca ccgaaagtta tgtcgtctta tacctggagt    960

ggcgatgata aagcaggtcc gccgatgcat agcgatggca ccacccgccc gaccgattgt   1020

agtgcagatc gctggctgtg tgaacatcgc gcagttgccg gcatggtggg ttttcataat   1080

gccgttgcag gccagggcat tggtagtgca gtgaccgatg gtaatggtcg cttagccttt   1140

gcacgcggta gcgcaggcta tgcagccttt aatgcaacca ataccgcctg gacccgcacc   1200

tttaccacct cactgccgga tggtgtgtat tgtgatgttg ccaatggcac ctttgtggat   1260

ggcgtgtgtg atggtccgag ctatcaggtg agcggcggca aatttaccgc aaccgttccg   1320

gccaatggtg ccgttgcctt acatgttgaa                                    1350


<210>  4
<211>  450
<212>  PRT
<213>  ˹

<400>  4

Ala Pro Ser Gly Asn Arg Asp Val Ile Val His Leu Phe Gln Trp Arg 
1               5                   10                  15      


Trp Lys Ser Ile Ala Asp Glu Cys Arg Thr Thr Leu Gly Pro His Gly 
            20                  25                  30          


Phe Gly Ala Val Gln Val Ser Pro Pro Gln Glu His Val Val Leu Pro 
        35                  40                  45              


Ala Glu Asp Tyr Pro Trp Trp Gln Asp Tyr Gln Pro Val Ser Tyr Lys 
    50                  55                  60                  


Leu Asp Gln Thr Arg Arg Gly Ser Arg Ala Asp Phe Ile Asp Met Val 
65                  70                  75                  80  


Asn Thr Cys Arg Glu Ala Gly Val Lys Ile Tyr Val Asp Ala Val Ile 
                85                  90                  95      


Asn His Met Thr Gly Thr Gly Ser Ala Gly Ala Gly Pro Gly Ser Ala 
            100                 105                 110         


Gly Ser Ser Tyr Ser Lys Tyr Asp Tyr Pro Gly Ile Tyr Gln Ser Gln 
        115                 120                 125             


Asp Phe Asn Asp Cys Arg Arg Asp Ile Thr Asn Trp Asn Asp Lys Trp 
    130                 135                 140                 


Glu Val Gln His Cys Glu Leu Val Gly Leu Ala Asp Leu Lys Thr Ser 
145                 150                 155                 160 


Ser Pro Tyr Val Gln Asp Arg Ile Ala Ala Tyr Leu Asn Glu Leu Ile 
                165                 170                 175     


Asp Leu Gly Val Ala Gly Phe Arg Ile Asp Ala Ala Lys His Ile Pro 
            180                 185                 190         


Glu Gly Asp Leu Gln Ala Ile Leu Ser Arg Leu Lys Asn Val His Pro 
        195                 200                 205             


Ala Trp Gly Gly Gly Lys Pro Tyr Ile Phe Gln Glu Val Ile Ala Asp 
    210                 215                 220                 


Ser Thr Ile Ser Thr Gly Ser Tyr Thr His Leu Gly Ser Val Thr Glu 
225                 230                 235                 240 


Phe Gln Tyr His Arg Asp Ile Ser His Ala Phe Ala Asn Gly Asn Ile 
                245                 250                 255     


Ala His Leu Thr Gly Leu Gly Ser Gly Leu Thr Pro Ser Asp Lys Ala 
            260                 265                 270         


Val Val Phe Val Val Asn His Asp Thr Gln Arg Tyr Glu Pro Ile Leu 
        275                 280                 285             


Thr His Thr Asp Gly Ala Arg Tyr Asp Leu Ala Gln Lys Phe Met Leu 
    290                 295                 300                 


Ala His Pro Tyr Gly Thr Pro Lys Val Met Ser Ser Tyr Thr Trp Ser 
305                 310                 315                 320 


Gly Asp Asp Lys Ala Gly Pro Pro Met His Ser Asp Gly Thr Thr Arg 
                325                 330                 335     


Pro Thr Asp Cys Ser Ala Asp Arg Trp Leu Cys Glu His Arg Ala Val 
            340                 345                 350         


Ala Gly Met Val Gly Phe His Asn Ala Val Ala Gly Gln Gly Ile Gly 
        355                 360                 365             


Ser Ala Val Thr Asp Gly Asn Gly Arg Leu Ala Phe Ala Arg Gly Ser 
    370                 375                 380                 


Ala Gly Tyr Ala Ala Phe Asn Ala Thr Asn Thr Ala Trp Thr Arg Thr 
385                 390                 395                 400 


Phe Thr Thr Ser Leu Pro Asp Gly Val Tyr Cys Asp Val Ala Asn Gly 
                405                 410                 415     


Thr Phe Val Asp Gly Val Cys Asp Gly Pro Ser Tyr Gln Val Ser Gly 
            420                 425                 430         


Gly Lys Phe Thr Ala Thr Val Pro Ala Asn Gly Ala Val Ala Leu His 
        435                 440                 445             


Val Glu 
    450 


<210>  5
<211>  36
<212>  DNA
<213>  ˹

<400>  5
aacatgttgt gaaaccggcc gaagattatc cgtggt                               36


<210>  6
<211>  33
<212>  DNA
<213>  ˹

<400>  6
tcttcggccg gtttcacaac atgttcctgc ggc                                  33


<210>  7
<211>  34
<212>  DNA
<213>  ˹

<400>  7
aacatgttgt gcgtccggcc gaagattatc cgtg                                 34


<210>  8
<211>  33
<212>  DNA
<213>  ˹

<400>  8
tcttcggccg gacgcacaac atgttcctgc ggc                                  33


<210>  9
<211>  29
<212>  DNA
<213>  ˹

<400>  9
tcacatgacc agcaccggct cagcaggcg                                       29


<210>  10
<211>  33
<212>  DNA
<213>  ˹

<400>  10
tgctgagccg gtgctggtca tgtgattaat aac                                  33


<210>  11
<211>  30
<212>  DNA
<213>  ˹

<400>  11
tcacatgacc accaccggct cagcaggcgc                                      30


<210>  12
<211>  33
<212>  DNA
<213>  ˹

<400>  12
tgctgagccg gtggtggtca tgtgattaat aac                                  33


<210>  13
<211>  29
<212>  DNA
<213>  ˹

<400>  13
tcacatgacc gataccggct cagcaggcg                                       29


<210>  14
<211>  36
<212>  DNA
<213>  ˹

<400>  14
tgctgagccg gtatcggtca tgtgattaat aacggc                               36


<210>  15
<211>  32
<212>  DNA
<213>  ˹

<400>  15
tcacatgacc aataccggct cagcaggcgc ag                                   32


<210>  16
<211>  37
<212>  DNA
<213>  ˹

<400>  16
ctgctgagcc ggtattggtc atgtgattaa taacggc                              37


<210>  17
<211>  26
<212>  DNA
<213>  ˹

<400>  17
tcacatgacc gaaaccggct cagcag                                          26


<210>  18
<211>  33
<212>  DNA
<213>  ˹

<400>  18
tgctgagccg gtttcggtca tgtgattaat aac                                  33


<210>  19
<211>  32
<212>  DNA
<213>  ˹

<400>  19
tcacatgacc cagaccggct cagcaggcgc ag                                   32


<210>  20
<211>  36
<212>  DNA
<213>  ˹

<400>  20
tgctgagccg gtctgggtca tgtgattaat aacggc                               36


<210>  21
<211>  27
<212>  DNA
<213>  ˹

<400>  21
accggaaccc cttcagcagg cgcaggt                                         27


<210>  22
<211>  34
<212>  DNA
<213>  ˹

<400>  22
gcgcctgctg aaggggttcc ggtcatgtga ttaa                                 34


<210>  23
<211>  25
<212>  DNA
<213>  ˹

<400>  23
accggaaccg tttcagcagg cgcag                                           25


<210>  24
<211>  24
<212>  DNA
<213>  ˹

<400>  24
gcgcctgctg aaacggttcc ggtc                                            24


<210>  25
<211>  26
<212>  DNA
<213>  ˹

<400>  25
gaccggaacc cactcagcag gcgcag                                          26


<210>  26
<211>  30
<212>  DNA
<213>  ˹

<400>  26
cgcctgctga gtgggttccg gtcatgtgat                                      30


<210>  27
<211>  41
<212>  DNA
<213>  ˹

<400>  27
gtgaattagt ggaattagca gacctgaaaa cctctagtcc g                         41


<210>  28
<211>  39
<212>  DNA
<213>  ˹

<400>  28
ggtctgctaa ttccactaat tcacaatgct gaacttccc                            39


<210>  29
<211>  41
<212>  DNA
<213>  ˹

<400>  29
gtgaattagt gcagttagca gacctgaaaa cctctagtcc g                         41


<210>  30
<211>  41
<212>  DNA
<213>  ˹

<400>  30
caggtctgct aactgcacta attcacaatg ctgaacttcc c                         41


<210>  31
<211>  40
<212>  DNA
<213>  ˹

<400>  31
tgaattagtg gacttagcag acctgaaaac ctctagtccg                           40


<210>  32
<211>  35
<212>  DNA
<213>  ˹

<400>  32
ggtctgctaa gtccactaat tcacaatgct gaact                                35


<210>  33
<211>  40
<212>  DNA
<213>  ˹

<400>  33
tgaattagtg aatttagcag acctgaaaac ctctagtccg                           40


<210>  34
<211>  35
<212>  DNA
<213>  ˹

<400>  34
ggtctgctaa attcactaat tcacaatgct gaact                                35


<210>  35
<211>  40
<212>  DNA
<213>  ˹

<400>  35
tgaattagtg agtttagcag acctgaaaac ctctagtccg                           40


<210>  36
<211>  34
<212>  DNA
<213>  ˹

<400>  36
ggtctgctaa actcactaat tcacaatgct gaac                                 34


<210>  37
<211>  41
<212>  DNA
<213>  ˹

<400>  37
gtgaattagt gcatttagca gacctgaaaa cctctagtcc g                         41


<210>  38
<211>  40
<212>  DNA
<213>  ˹

<400>  38
aggtctgcta aatgcactaa ttcacaatgc tgaacttccc                           40


