                         SEQUENCE LISTING

<110>  The Regents of the University of Michigan
 
<120>  PRODUCTS AND METHODS FOR THE TREATMENT OF NICOTINE DEPENDENCE

<130>  30275/55531A

<150>  US 63/032,403
<151>  2020-05-29

<160>  140   

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  1
tcctctaact gaaacttttg aaag                                              24


<210>  2
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  2
atccggggat ccgctctttt gacattatcc tctg                                   34


<210>  3
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  3
atccgggaat tcgttcatgt taagcagaat ctcg                                   34


<210>  4
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  4
ctttcaaaag tttcagttag aggacgcttg tttcattttt tatcctc                     47


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  5
gattcatcaa agaggggcag                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  6
gtattcgtta caggcagagc                                                   20


<210>  7
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  7

Met Thr Thr Phe Trp Ser Leu Tyr Ile Thr Ala Leu Thr Val Gly Thr 
1               5                   10                  15      


Leu Ile Ala Leu Leu Trp Leu Tyr Phe Ala Thr Arg Lys Gly Glu Ala 
            20                  25                  30          


Lys Gly Thr Thr Glu Lys Thr Met Gly His Ser Tyr Asp Gly Ile Glu 
        35                  40                  45              


Glu Tyr Asp Asn Pro Leu Pro Lys Trp Trp Phe Met Leu Tyr Ile Gly 
    50                  55                  60                  


Thr Ile Val Phe Ser Val Ser Tyr Leu Val Leu Tyr Pro Gly Leu Gly 
65                  70                  75                  80  


Asn Trp Lys Gly Val Leu Pro Gly Tyr Glu Gly Gly Trp Thr Gln Asp 
                85                  90                  95      


Lys Gln Trp Glu Arg Glu Met Asn Ile Ala Gln Glu Lys Tyr Gly Pro 
            100                 105                 110         


Ile Phe Ala Lys Tyr Ala Ala Met Pro Ile Glu Glu Val Ala Lys Asp 
        115                 120                 125             


Glu His Ala Met Lys Met Gly Ser Arg Met Phe Ala Thr Tyr Cys Ser 
    130                 135                 140                 


Ile Cys His Gly Ser Asp Ala Lys Gly Ala Leu Gly Phe Pro Asn Leu 
145                 150                 155                 160 


Ala Asp Asn Glu Trp Arg Trp Gly Gly Asp Pro Gln Ser Ile Glu Thr 
                165                 170                 175     


Thr Ile Leu Gly Gly Arg His Ala Ile Met Ala Ala Trp Gly Asp Ile 
            180                 185                 190         


Leu Gly Glu Asp Gly Val Lys Asn Val Ala Ala Tyr Val Arg Thr Glu 
        195                 200                 205             


Leu Ala Gly Leu Lys Leu Pro Glu Gly Thr Lys Ala Asp Val Glu Ala 
    210                 215                 220                 


Gly Lys Gln Ile Phe Ser Val Asn Cys Val Ala Cys His Gly Pro Glu 
225                 230                 235                 240 


Gly Lys Gly Thr Ala Leu Val Gly Ala Pro Asn Leu Thr Asn Pro Gly 
                245                 250                 255     


Ala Phe Ile Tyr Gly Ser Ser Tyr Ala Gln Leu Gln Gln Thr Ile Arg 
            260                 265                 270         


His Gly Arg Gln Gly Gln Met Pro Ala Gln Glu Pro Tyr Leu Gly Lys 
        275                 280                 285             


Glu Lys Val His Ile Leu Ala Ala Tyr Ile Tyr Asn Leu Ser His Asn 
    290                 295                 300                 


Gln Gly Ser Asn 
305             


<210>  8
<211>  312
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide

<400>  8

Met Thr Thr Phe Trp Ser Thr Tyr Ile Cys Val Leu Thr Ile Gly Ser 
1               5                   10                  15      


Leu Ile Gly Leu Thr Trp Leu Leu Leu Ala Thr Arg Lys Gly Gln Ser 
            20                  25                  30          


Asn Asn Thr Thr Asp Gln Thr Met Gly His Ser Phe Asp Gly Ile Glu 
        35                  40                  45              


Glu Tyr Asp Asn Pro Leu Pro Lys Trp Trp Phe Trp Leu Phe Val Gly 
    50                  55                  60                  


Thr Leu Val Phe Ser Val Gly Tyr Leu Ile Leu Tyr Pro Gly Leu Gly 
65                  70                  75                  80  


Asn Trp Lys Gly Ile Leu Pro Gly Tyr Glu Asn Gly Trp Thr Gly Ala 
                85                  90                  95      


Asn Glu Trp Gln Lys Glu Met Glu Lys Ala Asp Ala Arg Phe Gly Pro 
            100                 105                 110         


Ile Phe Ala Lys Tyr Ala Ala Met Pro Val Glu Glu Val Ala Lys Asp 
        115                 120                 125             


Pro Gln Ala Leu Lys Met Gly Ser Arg Leu Phe Ala Ser Asn Cys Ser 
    130                 135                 140                 


Val Cys His Gly Ser Asp Ala Lys Gly Ala Tyr Gly Phe Pro Asn Leu 
145                 150                 155                 160 


Thr Asp Lys Asp Trp Arg Trp Gly Gly Glu Ala Glu Thr Ile Lys Ala 
                165                 170                 175     


Ser Ile Met Asn Gly Arg His Gly Val Met Pro Ala Trp Ala Glu Val 
            180                 185                 190         


Ile Gly Glu Gln Gly Val Ala Val Ala Ala Phe Val Leu Thr Asn Leu 
        195                 200                 205             


Asp Gly Arg Ser Leu Pro Glu Gly Val Lys Ala Asp Ala Ala Lys Gly 
    210                 215                 220                 


Lys Glu Ile Phe Ala Gly Asn Cys Val Ala Cys His Gly Pro Glu Gly 
225                 230                 235                 240 


Lys Gly Thr Pro Ala Met Gly Ala Pro Asp Leu Thr His Pro Gln Ala 
                245                 250                 255     


Phe Ile Tyr Gly Ser Ser Phe Ala Gln Leu Gln Gln Thr Ile Arg Tyr 
            260                 265                 270         


Gly Arg Gln Gly Gln Met Pro Ala Gln Ala Glu Ile Gln Gly Asn Asp 
        275                 280                 285             


Lys Val His Leu Leu Ala Ala Tyr Val Tyr Ser Leu Ser Gln Asp Gln 
    290                 295                 300                 


Ala Ser Pro Asp Leu Thr Ala Lys 
305                 310         


<210>  9
<211>  324
<212>  PRT
<213>  Artificial Sequnce

<220>
<223>  Synthetic Polypeptide

<400>  9

Met Thr Thr Phe Trp Ser Leu Tyr Val Thr Val Leu Thr Leu Gly Thr 
1               5                   10                  15      


Ile Phe Ser Leu Thr Trp Leu Leu Leu Ser Thr Arg Lys Gly Gln Arg 
            20                  25                  30          


Glu Glu Val Thr Asp Glu Thr Val Gly His Ala Phe Asp Gly Ile Glu 
        35                  40                  45              


Glu Tyr Asp Asn Pro Leu Pro Lys Trp Trp Phe Trp Leu Phe Val Gly 
    50                  55                  60                  


Thr Ile Ile Phe Ala Leu Gly Tyr Leu Val Leu Tyr Pro Gly Leu Gly 
65                  70                  75                  80  


Asn Trp Lys Gly Val Leu Pro Gly Tyr Ser Tyr Leu Asp Asn Asp Lys 
                85                  90                  95      


Gln Thr Glu Phe Ser Asn Gly Gln Pro Gly Trp Thr Gly Val His Glu 
            100                 105                 110         


Gln Lys Glu Met Ala Lys Ala Asp Ala Arg Phe Gly Pro Ile Phe Ala 
        115                 120                 125             


Lys Phe Ala Ala Met Pro Ile Thr Glu Val Ala Lys Asp Pro Gln Ala 
    130                 135                 140                 


Leu Lys Met Gly Ala Arg Leu Phe Ala Ser Asn Cys Ser Val Cys His 
145                 150                 155                 160 


Gly Ser Asp Ala Lys Gly Ala Phe Gly Phe Pro Asn Leu Thr Asp Asn 
                165                 170                 175     


Asp Trp Arg Trp Gly Gly Glu Pro Asp Thr Ile Lys Thr Thr Ile Met 
            180                 185                 190         


Gly Gly Arg His Phe Val Met Pro Ala Trp Ala Glu Val Ile Gly Asp 
        195                 200                 205             


Gln Phe Val Ala Asp Val Ala Ala Phe Val Val Ser Lys Leu Asp Gly 
    210                 215                 220                 


Arg Thr Leu Pro Glu Gly Val Lys Ala Asp Ala Glu Asn Gly Gln Lys 
225                 230                 235                 240 


Ile Phe Ala Ala Asn Cys Val Ala Cys His Gly Pro Glu Gly Lys Gly 
                245                 250                 255     


Thr Pro Ala Met Gly Ala Pro Asn Leu Thr His Pro Gln Ala Phe Ile 
            260                 265                 270         


Tyr Gly Ser Ser Phe Ala Gln Leu Gln Gln Thr Ile Arg Tyr Gly Arg 
        275                 280                 285             


Gln Gly Gln Met Pro Ala Gln Glu Gln Leu Gln Gly Asn Asp Lys Val 
    290                 295                 300                 


His Leu Leu Ala Ala Tyr Val Tyr Ser Leu Ser Gln Gln Ala Glu Pro 
305                 310                 315                 320 


Ala Lys Ala Glu 
                


<210>  10
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Polypeptide


<220>
<221>  MISC_FEATURE
<222>  (9)..(9)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (29)..(29)
<223>  Xaa is D, E, N, Q or Z

<220>
<221>  MISC_FEATURE
<222>  (32)..(32)
<223>  Xaa is D, E, N, Q or Z

<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (38)..(38)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (56)..(56)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (57)..(57)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (61)..(61)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (68)..(68)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (79)..(79)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (84)..(84)
<223>  Xaa is D, E, N, Q, or Z

<220>
<221>  MISC_FEATURE
<222>  (89)..(89)
<223>  Xaa is D, E, N, Q, or Z

<220>
<221>  MISC_FEATURE
<222>  (91)..(91)
<223>  Xaa is D, E, N, Q, or Z

<220>
<221>  MISC_FEATURE
<222>  (97)..(97)
<223>  Xaa is D, E, N, Q, or Z

<220>
<221>  MISC_FEATURE
<222>  (100)..(100)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (107)..(107)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (112)..(112)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (122)..(122)
<223>  Xaa is L or M

<220>
<221>  MISC_FEATURE
<222>  (128)..(128)
<223>  Xaa is L or M

<220>
<221>  MISC_FEATURE
<222>  (135)..(135)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (153)..(153)
<223>  Xaa is D,E, N, Q, or  Z

<220>
<221>  MISC_FEATURE
<222>  (159)..(159)
<223>  Xaa is D,E, N, Q, or  Z

<220>
<221>  MISC_FEATURE
<222>  (161)..(161)
<223>  Xaa is D,E, N, Q, or  Z

<220>
<221>  MISC_FEATURE
<222>  (168)..(168)
<223>  Xaa is L or M

<220>
<221>  MISC_FEATURE
<222>  (174)..(174)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (180)..(180)
<223>  Xaa is D, E, N, Q, or Z

<220>
<221>  MISC_FEATURE
<222>  (181)..(181)
<223>  Xaa is I or V

<220>
<221>  MISC_FEATURE
<222>  (184)..(185)
<223>  Xaa is D, E, N, Q or Z

<220>
<221>  MISC_FEATURE
<222>  (189)..(189)
<223>  Xaa is D, E, N, Q or Z

<220>
<221>  MISC_FEATURE
<222>  (193)..(193)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (234)..(234)
<223>  Xaa is D,E, N, Q, or Z

<220>
<221>  MISC_FEATURE
<222>  (247)..(247)
<223>  Xaa is F or Y

<220>
<221>  MISC_FEATURE
<222>  (270)..(270)
<223>  Xaa is D, E, N,Q, Z

<220>
<221>  MISC_FEATURE
<222>  (279)..(279)
<223>  Xaa is I or V

<400>  10

Met Thr Thr Phe Trp Ser Leu Tyr Xaa Thr Val Leu Thr Gly Thr Leu 
1               5                   10                  15      


Ile Leu Thr Trp Leu Leu Leu Ala Thr Arg Lys Gly Xaa Thr Thr Xaa 
            20                  25                  30          


Thr Met Gly His Ser Xaa Asp Gly Ile Glu Glu Tyr Asp Asn Pro Leu 
        35                  40                  45              


Pro Lys Trp Trp Phe Trp Leu Xaa Xaa Gly Thr Ile Xaa Phe Ser Val 
    50                  55                  60                  


Gly Tyr Leu Xaa Leu Tyr Pro Gly Leu Gly Asn Trp Lys Gly Xaa Leu 
65                  70                  75                  80  


Pro Gly Tyr Xaa Gly Trp Thr Gly Xaa Trp Xaa Lys Glu Met Lys Ala 
                85                  90                  95      


Xaa Ala Arg Xaa Gly Pro Ile Phe Ala Lys Xaa Ala Ala Met Pro Xaa 
            100                 105                 110         


Glu Glu Val Ala Lys Asp Pro Gln Ala Xaa Lys Met Gly Ser Arg Xaa 
        115                 120                 125             


Phe Ala Ser Asn Cys Ser Xaa Cys His Gly Ser Asp Ala Lys Gly Ala 
    130                 135                 140                 


Gly Phe Pro Asn Leu Thr Asp Asn Xaa Trp Arg Trp Gly Gly Xaa Pro 
145                 150                 155                 160 


Xaa Thr Ile Lys Thr Thr Ile Xaa Gly Gly Arg His Gly Xaa Met Pro 
                165                 170                 175     


Ala Trp Ala Xaa Xaa Ile Gly Xaa Xaa Gly Val Ala Xaa Val Ala Ala 
            180                 185                 190         


Xaa Val Thr Leu Asp Gly Arg Leu Pro Glu Gly Val Lys Ala Asp Ala 
        195                 200                 205             


Glu Gly Lys Ile Phe Ala Asn Cys Val Ala Cys His Gly Pro Glu Gly 
    210                 215                 220                 


Lys Gly Thr Pro Ala Met Gly Ala Pro Xaa Leu Thr His Pro Gln Ala 
225                 230                 235                 240 


Phe Ile Tyr Gly Ser Ser Xaa Ala Gln Leu Gln Gln Thr Ile Arg Tyr 
                245                 250                 255     


Gly Arg Gln Gly Gln Met Pro Ala Gln Glu Gln Gly Asn Xaa Lys Val 
            260                 265                 270         


His Leu Leu Ala Ala Tyr Xaa Tyr Ser Leu Ser Gln Gln Ala Ser Pro 
        275                 280                 285             


<210>  11
<211>  490
<212>  PRT
<213>  Pseudomonas putida

<400>  11

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser Leu Glu His His His His His His 
                485                 490 


<210>  12
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  12
atgagcgata agaccaaaac caacgagggt ttcagccgtc gtagctttat tggcagcgcg       60

gcggttgtta ccgcgggtgt tgcgggtctg ggtgcgattg atgcggcgag cgcgacccag      120

aagaccaacc gtgcgagcac cgtgaaaggt ggcttcgatt acgacgtggt tgtggttggt      180

ggcggttttg cgggtgcgac cgcggcgcgt gagtgcggtc tgcagggtta tcgtaccctg      240

ctgctggaag cgcgtagccg tctgggcggt cgtaccttca ccagccgttt tgcgggtcaa      300

gagatcgagt tcggcggtgc gtgggttcat tggctgcagc cgcatgtgtg ggcggagatg      360

caacgttacg gcctgggtgt ggttgaagat ccgctgacca acctggacaa gaccctgatc      420

atgtataacg atggcagcgt tgagagcatt agcccggacg agttcggcaa gaacatccgt      480

attgcgtttg agaaactgtg ccacgacgcg tgggaagtgt tcccgcgtcc gcacgaaccg      540

atgtttaccg agcgtgcgcg tgaactggat aagagcagcg tgctggaccg tatcaaaacc      600

ctgggtctga gccgtctgca gcaagcgcag attaacagct acatggcgct gtatgcgggc      660

gagaccaccg ataagttcgg cctgccgggt gttctgaaac tgtttgcgtg cggcggttgg      720

aactacgatg cgtttatgga caccgaaacc cactatcgta tccagggtgg caccatcggc      780

ctgattaacg cgatgctgac cgacagcggt gcggaagtgc gtatgagcgt tccggtgacc      840

gcggttgaac aagtgaacgg cggtgtgaag attaaaaccg acgatgacga aatcattacc      900

gcgggtgtgg ttgtgatgac cgtgccgctg aacacctaca aacacatcgg cttcaccccg      960

gcgctgagca agggtaaaca gcgttttatt aaagagggtc aactgagcaa gggtgcgaaa     1020

ctgtacgttc acgtgaaaca gaacctgggc cgtgttttcg cgtttgcgga tgaacagcaa     1080

ccgctgaact gggtgcaaac ccacgattat agcgacgagc tgggcaccat cctgagcatc     1140

accattgcgc gtaaggaaac cattgacgtt aacgatcgtg acgcggttac ccgtgaggtg     1200

cagaaaatgt ttccgggtgt tgaggtgctg ggcaccgcgg cgtatgattg gaccgcggac     1260

ccgtttagcc tgggtgcgtg ggcggcgtat ggtgtgggtc agctgagccg tctgaaggat     1320

ctgcaagcgg cggagggtcg tatcctgttt gcgggtgcgg aaaccagcaa cggctggcac     1380

gcgaacattg atggtgcggt tgagagcggt ctgcgtgcgg gtcgtgaagt gaaacaactg     1440

ctgagctaa                                                             1449


<210>  13
<211>  431
<212>  PRT
<213>  Paenarthrobacter nicotinovorans


<220>
<221>  MISC_FEATURE
<222>  (166)..(166)
<223>  Target of Mutagenesis

<220>
<221>  MISC_FEATURE
<222>  (311)..(311)
<223>  Target of Mutagenesis

<220>
<221>  MISC_FEATURE
<222>  (326)..(326)
<223>  Target of Mutagenesis

<400>  13

Met Tyr Asp Ala Ile Val Val Gly Gly Gly Phe Ser Gly Leu Lys Ala 
1               5                   10                  15      


Ala Arg Asp Leu Thr Asn Ala Gly Lys Lys Val Leu Leu Leu Glu Gly 
            20                  25                  30          


Gly Glu Arg Leu Gly Gly Arg Ala Tyr Ser Arg Glu Ser Arg Asn Val 
        35                  40                  45              


Pro Gly Leu Arg Val Glu Ile Gly Gly Ala Tyr Leu His Arg Lys His 
    50                  55                  60                  


His Pro Arg Leu Ala Ala Glu Leu Asp Arg Tyr Gly Ile Pro Thr Ala 
65                  70                  75                  80  


Ala Ala Ser Glu Phe Thr Ser Phe Arg His Arg Leu Gly Pro Thr Ala 
                85                  90                  95      


Val Asp Gln Ala Phe Pro Ile Pro Gly Ser Glu Ala Val Ala Val Glu 
            100                 105                 110         


Ala Ala Thr Tyr Thr Leu Leu Arg Asp Ala His Arg Ile Asp Leu Glu 
        115                 120                 125             


Lys Gly Leu Glu Asn Gln Asp Leu Glu Asp Leu Asp Ile Pro Leu Asn 
    130                 135                 140                 


Glu Tyr Val Asp Lys Leu Asp Leu Pro Pro Val Ser Arg Gln Phe Leu 
145                 150                 155                 160 


Leu Ala Trp Ala Trp Asn Met Leu Gly Gln Pro Ala Asp Gln Ala Ser 
                165                 170                 175     


Ala Leu Trp Met Leu Gln Leu Val Ala Ala His His Tyr Ser Ile Leu 
            180                 185                 190         


Gly Val Val Leu Ser Leu Asp Glu Val Phe Ser Asn Gly Ser Ala Asp 
        195                 200                 205             


Leu Val Asp Ala Met Ser Gln Glu Ile Pro Glu Ile Arg Leu Gln Thr 
    210                 215                 220                 


Val Val Thr Gly Ile Asp Gln Ser Gly Asp Val Val Asn Val Thr Val 
225                 230                 235                 240 


Lys Asp Gly His Ala Phe Gln Ala His Ser Val Ile Val Ala Thr Pro 
                245                 250                 255     


Met Asn Thr Trp Arg Arg Ile Val Phe Thr Pro Ala Leu Pro Glu Arg 
            260                 265                 270         


Arg Arg Ser Val Ile Glu Glu Gly His Gly Gly Gln Gly Leu Lys Ile 
        275                 280                 285             


Leu Ile His Val Arg Gly Ala Glu Ala Gly Ile Glu Cys Val Gly Asp 
    290                 295                 300                 


Gly Ile Phe Pro Thr Leu Tyr Asp Tyr Cys Glu Val Ser Glu Ser Glu 
305                 310                 315                 320 


Arg Leu Leu Val Ala Phe Thr Asp Ser Gly Ser Phe Asp Pro Thr Asp 
                325                 330                 335     


Ile Gly Ala Val Lys Asp Ala Val Leu Tyr Tyr Leu Pro Glu Val Glu 
            340                 345                 350         


Val Leu Gly Ile Asp Tyr His Asp Trp Ile Ala Asp Pro Leu Phe Glu 
        355                 360                 365             


Gly Pro Trp Val Ala Pro Arg Val Gly Gln Phe Ser Arg Val His Lys 
    370                 375                 380                 


Glu Leu Gly Glu Pro Ala Gly Arg Ile His Phe Val Gly Ser Asp Val 
385                 390                 395                 400 


Ser Leu Glu Phe Pro Gly Tyr Ile Glu Gly Ala Leu Glu Thr Ala Glu 
                405                 410                 415     


Cys Ala Val Asn Ala Ile Leu His Ser His His His His His His 
            420                 425                 430     


<210>  14
<211>  1278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  14
atgtatgatg cgattgttgt gggtggcggt tttagcggcc tgaaagcggc gcgtgacctg       60

accaatgcgg gcaagaaagt gctgctgctg gagggtggcg aacgtctggg tggccgtgcg      120

tacagccgtg agagccgtaa cgtgccgggt ctgcgtgttg aaatcggtgg cgcgtacctg      180

caccgtaagc atcacccgcg tctggcggcg gagctggacc gttatggcat tccgaccgcg      240

gcggcgagcg agttcaccag ctttcgtcat cgtctgggtc cgaccgcggt ggatcaggcg      300

ttcccgattc cgggtagcga ggcggtggcg gttgaagcgg cgacctacac cctgctgcgt      360

gatgcgcacc gtatcgatct ggagaagggt ctggaaaacc aagacctgga ggacctggat      420

attccgctga acgaatatgt ggacaaactg gatctgccgc cggttagccg tcagttcctg      480

ctggcgtggg cgtggaacat gctgggtcag ccggcggacc aagcgagcgc gctgtggatg      540

ctgcaactgg ttgcggcgca ccactatagc atcctgggtg tggttctgag cctggatgag      600

gtgtttagca acggcagcgc ggacctggtt gatgcgatga gccaggagat cccggaaatt      660

cgtctgcaaa ccgtggttac cggtattgac cagagcggcg atgtggttaa cgtgaccgtt      720

aaggacggtc acgcgttcca ggcgcacagc gtgatcgttg cgaccccgat gaacacctgg      780

cgtcgtattg ttttcacccc ggcgctgccg gagcgtcgtc gtagcgtgat cgaggaaggt      840

cacggtggcc aaggcctgaa aatcctgatt cacgtgcgtg gtgcggaggc gggcatcgaa      900

tgcgttggtg acggcatttt tccgaccctg tacgattatt gcgaagtgag cgagagcgaa      960

cgtctgctgg ttgcgttcac cgacagcggt agctttgacc cgaccgatat cggcgcggtg     1020

aaggatgcgg ttctgtacta cctgccggaa gttgaggtgc tgggtatcga ctaccacgat     1080

tggattgcgg acccgctgtt tgagggtccg tgggtggcgc cgcgtgttgg ccaatttagc     1140

cgtgtgcaca aagagctggg tgaaccggcg ggccgtattc acttcgtggg tagcgatgtt     1200

agcctggaat ttccgggcta tattgaaggt gcgctggaga ccgcggaatg cgcggtgaat     1260

gcgatcctgc acagctaa                                                   1278


<210>  15
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  15
atgaaacaag cgctgcaagt ggttacccgt cattttggta gcctggttct gctggcgctg       60

agcggtgtgg cggttggtgc ggataattgc gacccggaga acggtaagaa agtgtaccag      120

atctgcagcg tttgccacag caacgatacc accggtgtgc acggcgcggc ggcgccgaac      180

ctgcacggtc tggaaggccg taaagtgggt agcgttccgg gcttcaaatt tagcagcgcg      240

ctgcgtgaca gcggtgatac ctggaccccg caacacctgg acaagttcct ggagaacccg      300

atggcggttt atccgctgac ccgtatggcg tttagcggcc tgaaaaatga aaaagaccgt      360

cgtgatgttc tgtgcttcct gagcaagagc agcaactaa                             399


<210>  16
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  16
atgagtgata aaacaaaaac aaatgaaggc tttagccgca ggtcttttat cggaagcgcg       60

gcagtcgtaa cagcaggtgt tgcgggattg ggagctattg atgcggcttc ggctacgcaa      120

aaaacgaacc gagcaagcac cgtcaaaggt ggcttcgatt acgatgtggt agtagttggt      180

ggagggtttg ctggcgcgac agccgcccgt gaatgtggtt tgcagggtta tcgaacgctt      240

ttattggaag cgaggtcccg cctaggtggt cgtacgttta cctcgcgctt tgcaggtcaa      300

gaaattgaat ttggcggggc atgggtgcac tggctgcagc cgcatgtttg ggcagaaatg      360

cagcgttacg gtctgggtgt agtggaagat ccacttacta atttagataa aaccttaatc      420

atgtataacg acggaagcgt cgaaagtatt tcgcccgatg aatttggcaa aaacattcga      480

atagcttttg aaaagctttg tcacgatgcc tgggaagtat ttcctcgtcc gcatgagccg      540

atgtttactg agcgcgctcg ggaattggat aaatcttctg ttcttgatcg catcaaaact      600

ttgggcttaa gtcggctgca acaggctcaa atcaatagtt acatggcctt gtatgcaggt      660

gagacaactg acaaatttgg cctgcctggt gtacttaagt tgtttgcatg cggcggttgg      720

aactatgacg ccttcatgga cactgaaact cattatagaa ttcaaggggg cacgataggc      780

ctcattaatg caatgttgac cgatagcggt gccgaggtcc gcatgtctgt gcccgtcact      840

gctgttgagc aagtcaatgg tggcgtcaaa atcaagaccg acgacgacga aattattacc      900

gccggagtgg tcgtaatgac agttccactc aatacgtata aacatatcgg ttttacgcct      960

gccctttcta aaggtaaaca acgattcatc aaagaggggc agcttagcaa aggtgctaag     1020

ctttatgttc atgttaagca gaatctcgga cgggtttttg cgtttgcgga tgaacagcaa     1080

cctttaaact gggtccagac gcacgattac agcgacgagt tggggacaat actgtcgatc     1140

accatcgctc gcaaagaaac aattgatgtg aatgaccgag atgctgtaac tcgcgaagtt     1200

caaaaaatgt ttccgggtgt tgaggttctt ggtacagcgg cttacgactg gacagctgat     1260

ccattttcct tgggggcatg ggcggcttat ggagtaggtc aactaagtcg tctcaaagat     1320

ctacaggcgg ctgaaggacg tattttattt gcaggagctg aaaccagtaa cggttggcac     1380

gcgaatatcg atggtgctgt tgaaagtgga ctacgtgccg gtagggaggt taagcagctc     1440

ttaagctag                                                             1449


<210>  17
<211>  1278
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  17
ttgtatgacg cgattgtggt tggtgggggt ttcagcgggc tgaaagcagc tcgcgatctt       60

accaacgccg gcaagaaggt acttctgctt gaaggcggtg aacgtctggg aggacgggcg      120

tattcccggg agtcccggaa tgtacctggc ctgcgggtcg agatcggtgg agcatacctt      180

caccggaagc accacccgcg tttggcagct gaattggacc ggtatggaat tcccactgca      240

gctgcaagcg agttcacctc gtttaggcat cgccttggac cgaccgcagt agaccaggca      300

tttcctattc ctggctcaga agcagttgcg gtggaagcag ccacctacac attgctgcgt      360

gatgcgcaca ggatagatct ggaaaaggga ttggaaaacc aggaccttga ggacctggac      420

atcccactga acgagtatgt tgacaaactc gatttgcccc ctgtttctag gcagttcttg      480

ctcgcttggg catggaacat gcttggccag ccggctgacc aagcatccgc gctttggatg      540

ctgcaacttg ttgcagcgca tcactacagc atcctaggtg ttgttctctc gctggatgaa      600

gttttttcca atggttccgc tgaccttgtc gacgcaatga gccaagaaat tcctgaaatc      660

agactgcaga ctgtcgtgac cggaatagac cagtctggcg atgtagtgaa cgtgacggtc      720

aaggatgggc atgccttcca ggctcattcc gtgattgttg ctactccaat gaacacttgg      780

cgaaggatcg ttttcacccc cgcacttcct gaacggcgca ggtcagtaat cgaggaaggg      840

catggtggtc agggtctgaa aattcttatc cacgtgcggg gagccgaggc aggaattgaa      900

tgcgtcggag acggaatatt tcccactctc tacgattatt gcgaggtgtc ggaaagcgaa      960

cgactgttgg tggctttcac ggactcagga tccttcgatc cgaccgatat cggcgcagtc     1020

aaggacgcgg tgctttacta cttgccggag gttgaagttc tgggtatcga ctaccacgac     1080

tggatcgcag acccgctttt cgaagggccc tgggtagcac cgcgcgtggg acaatttagt     1140

cgagtgcata aggaacttgg agagcccgct ggccggattc atttcgtagg ttccgatgtt     1200

tcgttagagt tccccggata tattgaaggc gcactggaaa ccgctgaatg cgcggtgaac     1260

gcaattctgc attcgtag                                                   1278


<210>  18
<211>  399
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Polynucleotide

<400>  18
atgaaacaag cgttgcaagt tgttactcgt cattttggtt cgttagttct tctagcatta       60

tctggtgtag ctgtaggtgc agataactgt gatccggaaa atgggaaaaa ggtttaccaa      120

atttgcagtg tgtgccactc aaatgacact actggtgttc acggcgcagc ggcgcccaat      180

ctacacggtc tggagggccg gaaagtcggc tcggtgccgg gtttcaaatt ttcttcagcg      240

ctacgtgatt ctggggacac ttggaccccg cagcatttag ataagttcct ggagaatcct      300

atggccgtat atcccctaac ccgtatggct ttctcaggcc tcaaaaatga aaaagatcga      360

cgcgacgtgc tttgtttttt gagcaaatcc tctaactga                             399


<210>  19
<211>  320
<212>  PRT
<213>  Streptomyces sp.

<400>  19

Met Ala Pro Met Asp Pro Ser Leu Val Thr Ser Val Glu Gln Ser Ser 
1               5                   10                  15      


Leu Thr Glu Leu Gly Glu Ile Asn Ile Asp Asp Met Ile Asp Tyr Val 
            20                  25                  30          


His Lys Asp Leu Lys Lys Leu Pro Glu Tyr Ile Asp Leu Tyr Lys Arg 
        35                  40                  45              


Tyr Phe Lys His Arg Trp Asn Ala Tyr Asp Leu Asp Phe Ser Gln Asp 
    50                  55                  60                  


Ala Ile Asp Trp Arg Thr Lys Met Thr Glu Glu Glu Arg Asp Ala Phe 
65                  70                  75                  80  


Lys Glu Ile Ala Ala Ala Phe His His Ala Glu Arg Gln Val Glu Ile 
                85                  90                  95      


Glu Leu Pro Val Phe Met Leu Ser Gly Ser Glu Asp Ala Lys Leu Tyr 
            100                 105                 110         


Leu Ser Thr Gln Ile Glu Asp Glu Ala Arg His Thr Leu Phe Phe Asp 
        115                 120                 125             


Arg Phe Tyr Arg Glu Val Val Gly Leu Pro Gly Asp Ser Val Ala Glu 
    130                 135                 140                 


Leu Leu Ala Ala Ser Phe Asp Tyr Val Pro Glu Ser Phe Val Gly Ser 
145                 150                 155                 160 


Phe Gly Leu Met Ala His Leu Ser Asp Asp Leu Arg Lys Asp Pro Asp 
                165                 170                 175     


Asn Gln Lys Leu Arg Val Arg Tyr Ala Thr Ala Tyr Phe Leu Trp Ile 
            180                 185                 190         


Glu Gly Val Leu Ala Leu Ser Ile Met Lys Ile Thr Leu Thr Tyr Cys 
        195                 200                 205             


Arg Thr Arg Gly Tyr Leu Pro Gly Tyr Tyr Thr Gly Phe Ile Ala Ser 
    210                 215                 220                 


Cys Arg Asp Glu Ala Arg His Val Gln Phe Gly Met Arg Phe Leu Arg 
225                 230                 235                 240 


Asp Ala Ile Gln Glu Asp Pro Arg Leu Val His Glu Val Tyr Glu Thr 
                245                 250                 255     


Leu Arg Thr Glu Leu Ala Met Asn Gly Gly Val Ser Lys Pro Ala Pro 
            260                 265                 270         


Leu Glu Pro Leu Gly Trp Asp Ala Glu Ala Val Thr Glu Phe Met Ile 
        275                 280                 285             


Arg Gln Val Gln Asn Lys Leu Ser Asp Val Gly Ile Asp Leu Pro Pro 
    290                 295                 300                 


Asp Ile Ala Ala Met Val Gln Asn Ile Gln Pro Glu Ile Ala Gly Gly 
305                 310                 315                 320 


<210>  20
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2

<400>  20

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  21
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut177; G145A, S192I, Q359L, Q366R, S379N

<400>  21

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Ala Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ile 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Leu Gln Pro Leu Asn Trp Val Arg Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  22
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mutH4

<400>  22

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Ser Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Gln Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Val Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  23
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mutD1

<400>  23

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Ala Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ile 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Leu Gln Pro Leu Asn Trp Val Arg Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  24
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mutH3

<400>  24

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Ser 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Ile Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  25
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut217; D130G, S379N

<400>  25

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  26
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut216; R44H, F104L, V147F, G156C, G317D, H368R, I382V

<400>  26

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn His Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Phe Glu Ser Ile Ser Pro Asp Glu Phe Cys Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Val Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  27
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut214; F104I, D130G, G156D, K199R, D295N, T381S

<400>  27

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Ile Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Asp Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Arg Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asn Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Ser Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  28
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut210; G50D, A98S, A426T, W427L

<400>  28

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Asp Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ser Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Thr Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  29
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut208; G411D, W427L

<400>  29

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Asp Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  30
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut204; A107T, D130V, I330T

<400>  30

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Val Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Thr Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  31
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut149; H368R

<400>  31

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  32
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut36

<400>  32

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Ser Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  33
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut96

<400>  33

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Gln Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  34
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut75

<400>  34

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asn Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  35
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  Nica2mut1

<400>  35

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asn Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Gln His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  36
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut5

<400>  36

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Val Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  37
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut138; S146G, H368R, V432M

<400>  37

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Gly Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Met 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  38
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut155; D130G, S379N

<400>  38

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  39
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut175; F104L, G317D, H368R

<400>  39

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  40
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut21

<400>  40

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Asn Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Arg Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  41
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut43

<400>  41

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Cys Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu His Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  42
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut106

<400>  42

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Asp Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly His Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  43
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut113; E103D, E184K

<400>  43

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Asp Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Lys Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  44
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut8

<400>  44

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Ser 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  45
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut12

<400>  45

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro Tyr Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Ala Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  46
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut66

<400>  46

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Ile Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Gly Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  47
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut112

<400>  47

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Glu Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Ala Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  48
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut61

<400>  48

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Ser Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Met Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  49
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut45

<400>  49

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu His Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  50
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut31

<400>  50

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu His Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Val Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  51
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut11

<400>  51

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Leu His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Gln Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  52
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut19

<400>  52

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Ser Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Pro Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Leu Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  53
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut20

<400>  53

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Cys Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Gln His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Asp Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  54
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut136; G50C, R393C

<400>  54

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Cys Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Cys Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  55
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut163; L135M, H368R, L449V

<400>  55

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Met Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  56
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut189; G333S, R351H, L424Q

<400>  56

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Ser Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly His Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Gln Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  57
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut194; F174V, G260S, W427L

<400>  57

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Val Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Ser Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  58
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut202; A45V, D130V, I388F

<400>  58

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Val Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Val Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Phe Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  59
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut22

<400>  59

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Asp Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Val Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  60
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut40

<400>  60

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Ile Asn Met Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Ser Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  61
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut123; Q75H, P131S, P152S, I159V

<400>  61

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu His Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Ser Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Ser Asp Glu Phe Gly Lys Asn Val Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  62
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut118; K137R, L217P, N457T

<400>  62

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Arg Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Pro Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Thr Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  63
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut9

<400>  63

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Val Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Val Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Asp Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  64
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut101

<400>  64

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Thr Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Lys Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Val Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  65
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut105

<400>  65

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Gln Asn Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ile Pro Asp Glu Phe Gly Lys Asn Ile His 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  66
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut173; D130N, V303A, N311S, A395V

<400>  66

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asn Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Ala Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Ser Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Val Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  67
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut6

<400>  67

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Ser Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu His Gly Tyr Gly Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Ser Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  68
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut17

<400>  68

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Ser Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Ile Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Asn Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Ile Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  69
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut119; S276N, K292N, K324E, R351H

<400>  69

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Asn Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Asn Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Glu Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly His Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  70
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut130; P131S, V277I, V282I

<400>  70

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Ser Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Ile Pro Val Thr Ala Ile Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  71
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut158; W108R, D130Y, T183I, M403I

<400>  71

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Arg Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Tyr Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Ile Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Ile Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  72
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut169; G50A, L217P, G260A, V345L

<400>  72

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Ala Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Pro Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Ala Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Leu Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  73
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut183; W108R, D130Y, K337M, R398L

<400>  73

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Arg Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Tyr Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Met Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Leu Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  74
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut198; E249G, H368R

<400>  74

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Gly Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  75
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut23

<400>  75

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Ala Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Ser Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Thr Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Ala Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  76
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut25

<400>  76

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Glu Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Glu 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly His Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Ser Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  77
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut10

<400>  77

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Asp Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Ile Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Pro 
465                 470                 475                 480 


Ala Glu Leu Ile 
                


<210>  78
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut117; P180L, 244V, T258A, N462S

<400>  78

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Leu Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Val Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Ala Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  79
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut64

<400>  79

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Gly Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Ser Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Ile Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp Leu Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  80
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut174; T42A, R78H, D130V, K225N, F245Y

<400>  80

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Ala Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr His Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Val Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Asn Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Tyr Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  81
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut145; D130G, K157M, V173A, K191M

<400>  81

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Met Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Ala Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Met Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  82
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut146; R85H, P229L, R351H, R437H

<400>  82

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala His Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Leu Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly His Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser His Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  83
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut152; T267I, W427L

<400>  83

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ile Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  84
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut147; G156C, T267S, W427L

<400>  84

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Cys Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ser Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  85
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut167; D130G, D196H, M246T, K331N

<400>  85

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu His Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Thr Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Asn Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  86
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut179; F104L, D130G, G270C, R393C

<400>  86

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Cys Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Cys Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  87
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut192; G99D, D130V, E454D

<400>  87

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Asp Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Val Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Asp Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  88
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut201; D130G, R187L, V231I, Y242N, E408D

<400>  88

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Leu Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Ile Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Asn Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Asp Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  89
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut35

<400>  89

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Ile Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Ile Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Val Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Tyr 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Ala Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  90
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut7

<400>  90

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Val Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Leu Glu Ser Ile Ser Pro Asp Glu Phe Ser Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ser Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Asp Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  91
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut65

<400>  91

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Arg Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Pro Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Ile Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Phe Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Val Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  92
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut137; V48A, F104L, D130Y,T267I, L435Q

<400>  92

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Ala 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Tyr Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ile Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Gln Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  93
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut144; D130G, P131Q, S192N, M306V,A473V

<400>  93

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Gln Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Asn 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Val Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Val Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  94
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut160; T39S, K157R, I161F,M246T, H368L, E373K

<400>  94

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Ser Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Arg Asn Ile Arg 
145                 150                 155                 160 


Phe Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Thr Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Leu 
        355                 360                 365             


Asp Tyr Ser Asp Lys Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  95
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut180; G156D, H168Q, K292R, K324E, R351H

<400>  95

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Asp Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys Gln Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Arg Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Glu Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly His Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  96
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut95

<400>  96

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Val Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser His 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asn Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Ile Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Pro 
465                 470                 475                 480 


Ala Glu Leu Ile 
                


<210>  97
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut191; F104I, D130G, G156D, K199R, D295N, T381S

<400>  97

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Ile Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Asp Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Arg Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asn Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Ser Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  98
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2E3; A107T, D130G, T267I, L335V,N462S

<400>  98

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ile Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Val Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  99
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2D5; K49N, F93L, F104L, V127M, D130S, L132R, F174C, 
       T319I, E454D

<400>  99

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Asn Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Leu Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Met Val 
        115                 120                 125             


Glu Ser Pro Arg Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Cys Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Ile Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Asp Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  100
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2B5; A107T, P114Q, G317D, H368R,D389E, N462S

<400>  100

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Gln His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Glu Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  101
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2E4; F104I, G317D, H368R, N462S

<400>  101

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Ile Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  102
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2F2; Q100H, A107T, L112Q, H115Y, H368R

<400>  102

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly His Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Gln 
            100                 105                 110         


Gln Pro Tyr Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  103
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2H3; S12I, A107T, D130G, T267I, N462S

<400>  103

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ile Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ile Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  104
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut2D9; T92A, F174V, E249G, A281T, I298V, F355L, H368R, 
       E454D

<400>  104

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Ala Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Val Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Gly Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Thr Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Val Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Leu Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Asp Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  105
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut249; A69V, A107T, D130Y, K199R, P278Q, D295N, T381S

<400>  105

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Val Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Tyr Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Arg Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Gln Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asn Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Ser Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  106
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut245; F104L, D130G, V231I, L335V, L349M, N462S

<400>  106

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Ile Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Val Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Met Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  107
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut244; G50S, F104L, A107T, D130G, G156D, S192C, F235L, 
       G239A, A426T, L435Q

<400>  107

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Ser Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Asp Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Cys 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Leu Ala Cys Gly Ala Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Thr Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Gln Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  108
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut240; F104L, D130G, E184G, M246T, P278S, I291V

<400>  108

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Gly Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Thr Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Ser Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Val Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  109
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut239; F104I, D130A

<400>  109

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Ile Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Ala Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  110
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut237; W108R, D130Y, T183I, G202D, S379N

<400>  110

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Arg Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Tyr Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Ile Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Asp Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  111
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut234; F104L, G317D, D357E, H368R

<400>  111

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Glu Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  112
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut233; F174V, G260S, W427L

<400>  112

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Val Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Ser Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  113
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut232; F174V, G260S, H368P, W427L

<400>  113

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Val Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Ser Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Pro 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Leu Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  114
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut229; F104I, D130G, G156D, K199R, A209T, F355L, H368Y

<400>  114

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Ile Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Asp Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Arg Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Thr Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Leu Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Tyr 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  115
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut228; K314Q, H368R, N462S

<400>  115

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Gln His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  116
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut223; W171C, D243N, Q366R, S379N, D394N

<400>  116

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Cys Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asn Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Arg Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asn Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  117
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut220; V48A, F104L, D130Y, M246T, H368L, E373K, L441M

<400>  117

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Ala 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Tyr Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Thr Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Leu 
        355                 360                 365             


Asp Tyr Ser Asp Lys Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Met Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  118
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut219; K157R, I161F, M246T, H368R, L449V

<400>  118

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Arg Asn Ile Arg 
145                 150                 155                 160 


Phe Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Thr Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  119
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut253; F104L, G317D, H368R, L449V, N462S

<400>  119

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  120
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut260; F104L, G317D, H368R, L449V, N462S, L481P, S482E, 
       483L, 484I

<400>  120

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Pro Glu Leu Ile 
                


<210>  121
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut254; A107T, D130G, T267I, N462S

<400>  121

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Ile Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  122
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut255; F104L, G317D, H368R, L449V, N462S

<400>  122

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  123
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut302; A107T, L112M, D357E, H368R, G406D, L449V, N462S

<400>  123

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Met 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Glu Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Asp Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  124
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut303; Y54F, V59I, F63L, A107T, V127M, D130S, L132R, F235I,
       T280K, G317D, E408D, P421L

<400>  124

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Phe Asp Val Val Val Ile Gly Gly Gly Leu Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Met Val 
        115                 120                 125             


Glu Ser Pro Arg Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Ile Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Lys Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Asp Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Leu Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  125
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut304; V48A, F52L, F104L, W171R, E249G, H368R, T381S, N462S

<400>  125

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Ala 
        35                  40                  45              


Lys Gly Gly Leu Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Arg Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Gly Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Ser Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  126
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut305; F93L, F104L, V127M, D130S, L132R, S213T, T222S, 
       A281T, L374M, M403I

<400>  126

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Leu Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Met Val 
        115                 120                 125             


Glu Ser Pro Arg Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Thr Tyr Met Ala Leu Tyr Ala Gly Glu Ser Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Thr Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Met Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Ile Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  127
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut306; F93L, F104L, V127M, D130S, L132R, I198F, K331N

<400>  127

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Leu Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Met Val 
        115                 120                 125             


Glu Ser Pro Arg Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Phe Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Asn Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  128
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut307; F104L, A107T, S146I, G317D, H368R, L449V, N462S

<400>  128

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ile Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  129
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut313; F104L, E129V, S192N, E249G, G317D, H368R, T381S, 
       N462S

<400>  129

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Val Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Asn 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Gly Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Ser Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  130
<211>  1449
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  Wild-type nicA2

<400>  130
atgagcgata agaccaaaac caacgagggt ttcagccgtc gtagctttat tggcagcgcg       60

gcggttgtta ccgcgggtgt tgcgggtctg ggtgcgattg atgcggcgag cgcgacccag      120

aagaccaacc gtgcgagcac cgtgaaaggt ggcttcgatt acgacgtggt tgtggttggt      180

ggcggttttg cgggtgcgac cgcggcgcgt gagtgcggtc tgcagggtta tcgtaccctg      240

ctgctggaag cgcgtagccg tctgggcggt cgtaccttca ccagccgttt tgcgggtcaa      300

gagatcgagt tcggcggtgc gtgggttcat tggctgcagc cgcatgtgtg ggcggagatg      360

caacgttacg gcctgggtgt ggttgaagat ccgctgacca acctggacaa gaccctgatc      420

atgtataacg atggcagcgt tgagagcatt agcccggacg agttcggcaa gaacatccgt      480

attgcgtttg agaaactgtg ccacgacgcg tgggaagtgt tcccgcgtcc gcacgaaccg      540

atgtttaccg agcgtgcgcg tgaactggat aagagcagcg tgctggaccg tatcaaaacc      600

ctgggtctga gccgtctgca gcaagcgcag attaacagct acatggcgct gtatgcgggc      660

gagaccaccg ataagttcgg cctgccgggt gttctgaaac tgtttgcgtg cggcggttgg      720

aactacgatg cgtttatgga caccgaaacc cactatcgta tccagggtgg caccatcggc      780

ctgattaacg cgatgctgac cgacagcggt gcggaagtgc gtatgagcgt tccggtgacc      840

gcggttgaac aagtgaacgg cggtgtgaag attaaaaccg acgatgacga aatcattacc      900

gcgggtgtgg ttgtgatgac cgtgccgctg aacacctaca aacacatcgg cttcaccccg      960

gcgctgagca agggtaaaca gcgttttatt aaagagggtc aactgagcaa gggtgcgaaa     1020

ctgtacgttc acgtgaaaca gaacctgggc cgtgttttcg cgtttgcgga tgaacagcaa     1080

ccgctgaact gggtgcaaac ccacgattat agcgacgagc tgggcaccat cctgagcatc     1140

accattgcgc gtaaggaaac cattgacgtt aacgatcgtg acgcggttac ccgtgaggtg     1200

cagaaaatgt ttccgggtgt tgaggtgctg ggcaccgcgg cgtatgattg gaccgcggac     1260

ccgtttagcc tgggtgcgtg ggcggcgtat ggtgtgggtc agctgagccg tctgaaggat     1320

ctgcaagcgg cggagggtcg tatcctgttt gcgggtgcgg aaaccagcaa cggctggcac     1380

gcgaacattg atggtgcggt tgagagcggt ctgcgtgcgg gtcgtgaagt gaaacaactg     1440

ctgagctaa                                                             1449


<210>  131
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  Wild type NicA2

<400>  131

Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe Ile 
1               5                   10                  15      


Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala Ile 
            20                  25                  30          


Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val Lys 
        35                  40                  45              


Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala Gly 
    50                  55                  60                  


Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu Leu 
65                  70                  75                  80  


Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg Phe 
                85                  90                  95      


Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu Gln 
            100                 105                 110         


Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val Glu 
        115                 120                 125             


Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp Gly 
    130                 135                 140                 


Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg Ile 
145                 150                 155                 160 


Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg Pro 
                165                 170                 175     


His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser Ser 
            180                 185                 190         


Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln Ala 
        195                 200                 205             


Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp Lys 
    210                 215                 220                 


Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp Asn 
225                 230                 235                 240 


Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly Gly 
                245                 250                 255     


Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu Val 
            260                 265                 270         


Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly Val 
        275                 280                 285             


Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val Val 
    290                 295                 300                 


Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro Ala 
305                 310                 315                 320 


Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser Lys 
                325                 330                 335     


Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val Phe 
            340                 345                 350         


Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His Asp 
        355                 360                 365             


Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg Lys 
    370                 375                 380                 


Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val Gln 
385                 390                 395                 400 


Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp Trp 
                405                 410                 415     


Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val Gly 
            420                 425                 430         


Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile Leu 
        435                 440                 445             


Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp Gly 
    450                 455                 460                 


Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu Leu 
465                 470                 475                 480 


Ser 
    


<210>  132
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut314; G51S, G73S, R96S, F104L, A107T, L112M, E249G, S276C,
       H368R, G411S, N462S

<400>  132

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Ser Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Ser Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Ser 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Met 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Gly Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Cys Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Ser Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  133
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut315; A107T, L112M, F235Y, G260S, A339V, Q359R, H368R, 
       N462S

<400>  133

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Thr Trp Val His Trp Met 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Tyr Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Ser Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Val Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Arg Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  134
<211>  484
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut320; F104L, A107T, D130S, T319I, L449V, N462S, L480P, 
       L481A, S482E

<400>  134

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Ser Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Ile Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Pro 
465                 470                 475                 480 


Ala Glu Leu Ile 
                


<210>  135
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut321; F104L, A107T, S146I, K191N, Q210H, G317D, H368R, 
       L449V, N462S

<400>  135

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asp Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ile Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Asn Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala His Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Asp Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  136
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut323; F104L, A107T, D130S, M215L, E221D, T319I, L449V, 
       N462S

<400>  136

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Ser Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Leu Ala Leu Tyr Ala Gly Asp Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Ile Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  137
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut324; F104L, D130N, H368R, N462S

<400>  137

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Asn Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  138
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  nicA2mut325; D130G, D296E, G317S, Q359E, S371R, S379N, T418S, 
       L449V

<400>  138

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Phe Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Gly Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Glu Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Ser Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Glu Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Arg Asp Glu Leu Gly Thr Ile Leu Asn Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Ser Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Asn Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  139
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut326; F104L, D130S, H368R, N462S

<400>  139

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Ala Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Ser Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Thr Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Thr Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Thr Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr Arg 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Leu Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


<210>  140
<211>  482
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  MISC_FEATURE
<223>  NicA2mut329; F104L, A107T, D130S, T293S, T307P, T319I, L449V, 
       N462S

<400>  140

Met Ser Asp Lys Thr Lys Thr Asn Glu Gly Phe Ser Arg Arg Ser Phe 
1               5                   10                  15      


Ile Gly Ser Ala Ala Val Val Thr Ala Gly Val Ala Gly Leu Gly Ala 
            20                  25                  30          


Ile Asp Ala Ala Ser Ala Thr Gln Lys Thr Asn Arg Ala Ser Thr Val 
        35                  40                  45              


Lys Gly Gly Phe Asp Tyr Asp Val Val Val Val Gly Gly Gly Phe Ala 
    50                  55                  60                  


Gly Ala Thr Ala Ala Arg Glu Cys Gly Leu Gln Gly Tyr Arg Thr Leu 
65                  70                  75                  80  


Leu Leu Glu Ala Arg Ser Arg Leu Gly Gly Arg Thr Phe Thr Ser Arg 
                85                  90                  95      


Phe Ala Gly Gln Glu Ile Glu Leu Gly Gly Thr Trp Val His Trp Leu 
            100                 105                 110         


Gln Pro His Val Trp Ala Glu Met Gln Arg Tyr Gly Leu Gly Val Val 
        115                 120                 125             


Glu Ser Pro Leu Thr Asn Leu Asp Lys Thr Leu Ile Met Tyr Asn Asp 
    130                 135                 140                 


Gly Ser Val Glu Ser Ile Ser Pro Asp Glu Phe Gly Lys Asn Ile Arg 
145                 150                 155                 160 


Ile Ala Phe Glu Lys Leu Cys His Asp Ala Trp Glu Val Phe Pro Arg 
                165                 170                 175     


Pro His Glu Pro Met Phe Thr Glu Arg Ala Arg Glu Leu Asp Lys Ser 
            180                 185                 190         


Ser Val Leu Asp Arg Ile Lys Thr Leu Gly Leu Ser Arg Leu Gln Gln 
        195                 200                 205             


Ala Gln Ile Asn Ser Tyr Met Ala Leu Tyr Ala Gly Glu Thr Thr Asp 
    210                 215                 220                 


Lys Phe Gly Leu Pro Gly Val Leu Lys Leu Phe Ala Cys Gly Gly Trp 
225                 230                 235                 240 


Asn Tyr Asp Ala Phe Met Asp Thr Glu Thr His Tyr Arg Ile Gln Gly 
                245                 250                 255     


Gly Thr Ile Gly Leu Ile Asn Ala Met Leu Thr Asp Ser Gly Ala Glu 
            260                 265                 270         


Val Arg Met Ser Val Pro Val Thr Ala Val Glu Gln Val Asn Gly Gly 
        275                 280                 285             


Val Lys Ile Lys Ser Asp Asp Asp Glu Ile Ile Thr Ala Gly Val Val 
    290                 295                 300                 


Val Met Pro Val Pro Leu Asn Thr Tyr Lys His Ile Gly Phe Ile Pro 
305                 310                 315                 320 


Ala Leu Ser Lys Gly Lys Gln Arg Phe Ile Lys Glu Gly Gln Leu Ser 
                325                 330                 335     


Lys Gly Ala Lys Leu Tyr Val His Val Lys Gln Asn Leu Gly Arg Val 
            340                 345                 350         


Phe Ala Phe Ala Asp Glu Gln Gln Pro Leu Asn Trp Val Gln Thr His 
        355                 360                 365             


Asp Tyr Ser Asp Glu Leu Gly Thr Ile Leu Ser Ile Thr Ile Ala Arg 
    370                 375                 380                 


Lys Glu Thr Ile Asp Val Asn Asp Arg Asp Ala Val Thr Arg Glu Val 
385                 390                 395                 400 


Gln Lys Met Phe Pro Gly Val Glu Val Leu Gly Thr Ala Ala Tyr Asp 
                405                 410                 415     


Trp Thr Ala Asp Pro Phe Ser Leu Gly Ala Trp Ala Ala Tyr Gly Val 
            420                 425                 430         


Gly Gln Leu Ser Arg Leu Lys Asp Leu Gln Ala Ala Glu Gly Arg Ile 
        435                 440                 445             


Val Phe Ala Gly Ala Glu Thr Ser Asn Gly Trp His Ala Ser Ile Asp 
    450                 455                 460                 


Gly Ala Val Glu Ser Gly Leu Arg Ala Gly Arg Glu Val Lys Gln Leu 
465                 470                 475                 480 


Leu Ser 
        


