                        Sequence Listing for Steviol Glycosyltransferases and Genes Encoding the Same

<110>  QTG Development, Inc.
 
<120>  STEVIOL GLYCOSYLTRANSFERASES AND GENES ENCODING THE SAME

<130>  2189.314530

<140>  US 16/887,435
<141>  2020-05-29

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1479
<212>  DNA
<213>  Stevia rebaudiana


<220>
<221>  exon
<222>  (1)..(1479)

<400>  1
atg gcc acc aac aaa gtc cat ttc ctt cta att ccc cac ata ggc ccc         48
Met Ala Thr Asn Lys Val His Phe Leu Leu Ile Pro His Ile Gly Pro           
1               5                   10                  15                

ggt cac acg atc ccc atg atc gac atg gct aaa ctg ctc gct aaa caa         96
Gly His Thr Ile Pro Met Ile Asp Met Ala Lys Leu Leu Ala Lys Gln           
            20                  25                  30                    

cca aac gtt acg gtc acc atc gcc gtc aca ccg ctc aac gcc gcc cgt        144
Pro Asn Val Thr Val Thr Ile Ala Val Thr Pro Leu Asn Ala Ala Arg           
        35                  40                  45                        

tac ggt ggc acc ctc gcc gga gcc atc acc gcc gga ctt ccg gtc cgg        192
Tyr Gly Gly Thr Leu Ala Gly Ala Ile Thr Ala Gly Leu Pro Val Arg           
    50                  55                  60                            

ttt ttt gag ctc cca ttt ccg gca gta gag gct gga ttg cct gaa ggg        240
Phe Phe Glu Leu Pro Phe Pro Ala Val Glu Ala Gly Leu Pro Glu Gly           
65                  70                  75                  80            

tgt gaa agc aca gat caa atc cca agt gtg gtt tta atc cca aat ttt        288
Cys Glu Ser Thr Asp Gln Ile Pro Ser Val Val Leu Ile Pro Asn Phe           
                85                  90                  95                

tta tcc gcc att gat atg tta cag caa aag ctt gaa gaa cgg ttt gaa        336
Leu Ser Ala Ile Asp Met Leu Gln Gln Lys Leu Glu Glu Arg Phe Glu           
            100                 105                 110                   

atg ata aac cct cgt ccg aat tgc atc ata tct gat aaa tac atg tca        384
Met Ile Asn Pro Arg Pro Asn Cys Ile Ile Ser Asp Lys Tyr Met Ser           
        115                 120                 125                       

tgg acg ggt gat ttt gcg gat aag tat cgg tta ccg aga atc atg ttt        432
Trp Thr Gly Asp Phe Ala Asp Lys Tyr Arg Leu Pro Arg Ile Met Phe           
    130                 135                 140                           

gat gga atg agt tgt ttt aac gag tta tgt tgt aat aat ttg tat gaa        480
Asp Gly Met Ser Cys Phe Asn Glu Leu Cys Cys Asn Asn Leu Tyr Glu           
145                 150                 155                 160           

cac aag gtg ttc gac ggt ttg cct gac tca gaa cca ttt gtt gtc ctg        528
His Lys Val Phe Asp Gly Leu Pro Asp Ser Glu Pro Phe Val Val Leu           
                165                 170                 175               

ggt tta cct gat cgg gtt gag ttg acc aga aac cag ctc cca ccg gag        576
Gly Leu Pro Asp Arg Val Glu Leu Thr Arg Asn Gln Leu Pro Pro Glu           
            180                 185                 190                   

ttt aac ccg agc tcg gtt gac acg agt gca ttt cgc caa cgc gct aga        624
Phe Asn Pro Ser Ser Val Asp Thr Ser Ala Phe Arg Gln Arg Ala Arg           
        195                 200                 205                       

gat gct gaa gtt agg gct tat ggg gtg gtg att aat agt ttt gat gaa        672
Asp Ala Glu Val Arg Ala Tyr Gly Val Val Ile Asn Ser Phe Asp Glu           
    210                 215                 220                           

ctt gaa caa gaa tat gtt aat gag tat aag aaa tta aga ggg ggt aaa        720
Leu Glu Gln Glu Tyr Val Asn Glu Tyr Lys Lys Leu Arg Gly Gly Lys           
225                 230                 235                 240           

gtt tgg tgt atc gga cca ttg tcg cta tgc gat agc gac gat tcg att        768
Val Trp Cys Ile Gly Pro Leu Ser Leu Cys Asp Ser Asp Asp Ser Ile           
                245                 250                 255               

aaa tct caa agg gga aat gta gcc tca att aac gaa caa caa tgc cta        816
Lys Ser Gln Arg Gly Asn Val Ala Ser Ile Asn Glu Gln Gln Cys Leu           
            260                 265                 270                   

aag tgg ctt gat tct cac gaa cca gag tca gta gta tac gcg tgt ttt        864
Lys Trp Leu Asp Ser His Glu Pro Glu Ser Val Val Tyr Ala Cys Phe           
        275                 280                 285                       

ggt agt ttg gtt agg atc aat acg cct caa ctt att gag ctc ggt tta        912
Gly Ser Leu Val Arg Ile Asn Thr Pro Gln Leu Ile Glu Leu Gly Leu           
    290                 295                 300                           

ggc cta gaa gca tca aat cgc ccg ttc att tgg gtg att aaa tcg gtt        960
Gly Leu Glu Ala Ser Asn Arg Pro Phe Ile Trp Val Ile Lys Ser Val           
305                 310                 315                 320           

cat aga gaa aag gaa gtt gag gaa tgg tta gcc gaa agc ggg ttt gag       1008
His Arg Glu Lys Glu Val Glu Glu Trp Leu Ala Glu Ser Gly Phe Glu           
                325                 330                 335               

gag agg gtt aaa cat aga ggt ttg ata ata cgc ggt tgg gcc cca caa       1056
Glu Arg Val Lys His Arg Gly Leu Ile Ile Arg Gly Trp Ala Pro Gln           
            340                 345                 350                   

gtg cta atc ttg tcc cac ccg tca gtt gga ggg ttc tta aca cat tgt       1104
Val Leu Ile Leu Ser His Pro Ser Val Gly Gly Phe Leu Thr His Cys           
        355                 360                 365                       

ggt tgg aac tcg act cta gaa tca gtg tct gct ggt gtt ccc atg atc       1152
Gly Trp Asn Ser Thr Leu Glu Ser Val Ser Ala Gly Val Pro Met Ile           
    370                 375                 380                           

aca tgg cca cag ttc gca gag cag ttt att aac gag aag ctt gtg gtg       1200
Thr Trp Pro Gln Phe Ala Glu Gln Phe Ile Asn Glu Lys Leu Val Val           
385                 390                 395                 400           

caa gtt ttg ggg atc ggt gtc ggt gtt gga gct gat tcg gtt gtt cat       1248
Gln Val Leu Gly Ile Gly Val Gly Val Gly Ala Asp Ser Val Val His           
                405                 410                 415               

gtg ggt gaa gaa gat cgg ttt ggg gtg aaa gtt aag agt gag agc gtg       1296
Val Gly Glu Glu Asp Arg Phe Gly Val Lys Val Lys Ser Glu Ser Val           
            420                 425                 430                   

aag aag gct atc gag cag gtg atg gat ggc ggg att gaa gga aat gag       1344
Lys Lys Ala Ile Glu Gln Val Met Asp Gly Gly Ile Glu Gly Asn Glu           
        435                 440                 445                       

aga cga aag agc gtt aaa aaa ctc gcg ata att gcg aat aac gcg ata       1392
Arg Arg Lys Ser Val Lys Lys Leu Ala Ile Ile Ala Asn Asn Ala Ile           
    450                 455                 460                           

aag gag ggt gga tct tct cat ttg aac ttg acc cta cta att caa gac       1440
Lys Glu Gly Gly Ser Ser His Leu Asn Leu Thr Leu Leu Ile Gln Asp           
465                 470                 475                 480           

ata atg cat cat tcg gat act tca tgt aaa gag tct tga                   1479
Ile Met His His Ser Asp Thr Ser Cys Lys Glu Ser                           
                485                 490                                   


<210>  2
<211>  492
<212>  PRT
<213>  Stevia rebaudiana


<220>
<221>  PEPTIDE
<222>  (1)..(492)

<400>  2

Met Ala Thr Asn Lys Val His Phe Leu Leu Ile Pro His Ile Gly Pro 
1               5                   10                  15      


Gly His Thr Ile Pro Met Ile Asp Met Ala Lys Leu Leu Ala Lys Gln 
            20                  25                  30          


Pro Asn Val Thr Val Thr Ile Ala Val Thr Pro Leu Asn Ala Ala Arg 
        35                  40                  45              


Tyr Gly Gly Thr Leu Ala Gly Ala Ile Thr Ala Gly Leu Pro Val Arg 
    50                  55                  60                  


Phe Phe Glu Leu Pro Phe Pro Ala Val Glu Ala Gly Leu Pro Glu Gly 
65                  70                  75                  80  


Cys Glu Ser Thr Asp Gln Ile Pro Ser Val Val Leu Ile Pro Asn Phe 
                85                  90                  95      


Leu Ser Ala Ile Asp Met Leu Gln Gln Lys Leu Glu Glu Arg Phe Glu 
            100                 105                 110         


Met Ile Asn Pro Arg Pro Asn Cys Ile Ile Ser Asp Lys Tyr Met Ser 
        115                 120                 125             


Trp Thr Gly Asp Phe Ala Asp Lys Tyr Arg Leu Pro Arg Ile Met Phe 
    130                 135                 140                 


Asp Gly Met Ser Cys Phe Asn Glu Leu Cys Cys Asn Asn Leu Tyr Glu 
145                 150                 155                 160 


His Lys Val Phe Asp Gly Leu Pro Asp Ser Glu Pro Phe Val Val Leu 
                165                 170                 175     


Gly Leu Pro Asp Arg Val Glu Leu Thr Arg Asn Gln Leu Pro Pro Glu 
            180                 185                 190         


Phe Asn Pro Ser Ser Val Asp Thr Ser Ala Phe Arg Gln Arg Ala Arg 
        195                 200                 205             


Asp Ala Glu Val Arg Ala Tyr Gly Val Val Ile Asn Ser Phe Asp Glu 
    210                 215                 220                 


Leu Glu Gln Glu Tyr Val Asn Glu Tyr Lys Lys Leu Arg Gly Gly Lys 
225                 230                 235                 240 


Val Trp Cys Ile Gly Pro Leu Ser Leu Cys Asp Ser Asp Asp Ser Ile 
                245                 250                 255     


Lys Ser Gln Arg Gly Asn Val Ala Ser Ile Asn Glu Gln Gln Cys Leu 
            260                 265                 270         


Lys Trp Leu Asp Ser His Glu Pro Glu Ser Val Val Tyr Ala Cys Phe 
        275                 280                 285             


Gly Ser Leu Val Arg Ile Asn Thr Pro Gln Leu Ile Glu Leu Gly Leu 
    290                 295                 300                 


Gly Leu Glu Ala Ser Asn Arg Pro Phe Ile Trp Val Ile Lys Ser Val 
305                 310                 315                 320 


His Arg Glu Lys Glu Val Glu Glu Trp Leu Ala Glu Ser Gly Phe Glu 
                325                 330                 335     


Glu Arg Val Lys His Arg Gly Leu Ile Ile Arg Gly Trp Ala Pro Gln 
            340                 345                 350         


Val Leu Ile Leu Ser His Pro Ser Val Gly Gly Phe Leu Thr His Cys 
        355                 360                 365             


Gly Trp Asn Ser Thr Leu Glu Ser Val Ser Ala Gly Val Pro Met Ile 
    370                 375                 380                 


Thr Trp Pro Gln Phe Ala Glu Gln Phe Ile Asn Glu Lys Leu Val Val 
385                 390                 395                 400 


Gln Val Leu Gly Ile Gly Val Gly Val Gly Ala Asp Ser Val Val His 
                405                 410                 415     


Val Gly Glu Glu Asp Arg Phe Gly Val Lys Val Lys Ser Glu Ser Val 
            420                 425                 430         


Lys Lys Ala Ile Glu Gln Val Met Asp Gly Gly Ile Glu Gly Asn Glu 
        435                 440                 445             


Arg Arg Lys Ser Val Lys Lys Leu Ala Ile Ile Ala Asn Asn Ala Ile 
    450                 455                 460                 


Lys Glu Gly Gly Ser Ser His Leu Asn Leu Thr Leu Leu Ile Gln Asp 
465                 470                 475                 480 


Ile Met His His Ser Asp Thr Ser Cys Lys Glu Ser 
                485                 490         


<210>  3
<211>  1491
<212>  DNA
<213>  Stevia rebaudiana


<220>
<221>  exon
<222>  (1)..(1491)

<400>  3
atg gct tta gaa gaa tca caa gaa ccc aac caa ctt cac ttt ctt gta         48
Met Ala Leu Glu Glu Ser Gln Glu Pro Asn Gln Leu His Phe Leu Val           
1               5                   10                  15                

ata ccc tta gga tct cca ggt cac tat atc cca acc att gat tta tcc         96
Ile Pro Leu Gly Ser Pro Gly His Tyr Ile Pro Thr Ile Asp Leu Ser           
            20                  25                  30                    

aaa tta cta gct caa cat gga gtt aga gtc acc ata gtc acc acc ccg        144
Lys Leu Leu Ala Gln His Gly Val Arg Val Thr Ile Val Thr Thr Pro           
        35                  40                  45                        

gtt aac gcc gtc aga ttc ggg tca atc ctt gat caa gca att caa tcg        192
Val Asn Ala Val Arg Phe Gly Ser Ile Leu Asp Gln Ala Ile Gln Ser           
    50                  55                  60                            

ggt ctt ccc att agt ttt ctt gaa ttt cgg tta cca tat atg aag ttt        240
Gly Leu Pro Ile Ser Phe Leu Glu Phe Arg Leu Pro Tyr Met Lys Phe           
65                  70                  75                  80            

aac ata cca gaa ggt tgt gaa tgc ttg gat gat gtt cct aat att ggg        288
Asn Ile Pro Glu Gly Cys Glu Cys Leu Asp Asp Val Pro Asn Ile Gly           
                85                  90                  95                

tca gcc cat gat ctt ttt ctt gca cat agt tca ctg caa caa gaa gtt        336
Ser Ala His Asp Leu Phe Leu Ala His Ser Ser Leu Gln Gln Glu Val           
            100                 105                 110                   

gaa gaa tat att gaa aag ctt gat cat aaa cca agt tgc ata ctt tca        384
Glu Glu Tyr Ile Glu Lys Leu Asp His Lys Pro Ser Cys Ile Leu Ser           
        115                 120                 125                       

ggc aca tat ctt tta tgg aca agt gaa act gca aaa aag ttt cag att        432
Gly Thr Tyr Leu Leu Trp Thr Ser Glu Thr Ala Lys Lys Phe Gln Ile           
    130                 135                 140                           

cca agg att gtg ttt gat gga atg aat tgc ttc act cag atg tgt aac        480
Pro Arg Ile Val Phe Asp Gly Met Asn Cys Phe Thr Gln Met Cys Asn           
145                 150                 155                 160           

cat gtt tta tac atc tca aag gtg tat gaa agt gtt agt gag tca gaa        528
His Val Leu Tyr Ile Ser Lys Val Tyr Glu Ser Val Ser Glu Ser Glu           
                165                 170                 175               

tct ttt gtg tta ccc ggt ttg cct gat cgt att gaa cta aca aga tcc        576
Ser Phe Val Leu Pro Gly Leu Pro Asp Arg Ile Glu Leu Thr Arg Ser           
            180                 185                 190                   

caa cta tct ttt gta ttc aat tcg ggc tcc aaa gac gtg aag gat ttc        624
Gln Leu Ser Phe Val Phe Asn Ser Gly Ser Lys Asp Val Lys Asp Phe           
        195                 200                 205                       

agt gag aag ctt cgg gta tcg gag tcc gaa gcg ttt ggt ata gtt ata        672
Ser Glu Lys Leu Arg Val Ser Glu Ser Glu Ala Phe Gly Ile Val Ile           
    210                 215                 220                           

aat agt ttt cat gag ttg gag caa gaa tat gtt gag gca tat caa aaa        720
Asn Ser Phe His Glu Leu Glu Gln Glu Tyr Val Glu Ala Tyr Gln Lys           
225                 230                 235                 240           

gtc aaa gaa gat aaa gct tgg tgc ata ggg cca tta tct cta tgc cac        768
Val Lys Glu Asp Lys Ala Trp Cys Ile Gly Pro Leu Ser Leu Cys His           
                245                 250                 255               

aag gat gta tcc gag aaa gtc caa aga ggt aac aag tcc tca att gac        816
Lys Asp Val Ser Glu Lys Val Gln Arg Gly Asn Lys Ser Ser Ile Asp           
            260                 265                 270                   

aaa gat gaa tgc atc aag tgg ctc gat tct caa gaa aat gaa tcg gta        864
Lys Asp Glu Cys Ile Lys Trp Leu Asp Ser Gln Glu Asn Glu Ser Val           
        275                 280                 285                       

atc tat gtg tgt tta ggt agc atc agt cgc ctt gag cct tca cag ctc        912
Ile Tyr Val Cys Leu Gly Ser Ile Ser Arg Leu Glu Pro Ser Gln Leu           
    290                 295                 300                           

ata gag ctt gct tta ggt ctt gag tca tca aaa aga ccg ttc att tgg        960
Ile Glu Leu Ala Leu Gly Leu Glu Ser Ser Lys Arg Pro Phe Ile Trp           
305                 310                 315                 320           

gtg gtt cga gcc ggc cac aag act gag aag ata gaa aag tgg ata gat       1008
Val Val Arg Ala Gly His Lys Thr Glu Lys Ile Glu Lys Trp Ile Asp           
                325                 330                 335               

gaa gag ggg ttt gaa gag aga acc aaa gat aga ggt cta ttg atc cgc       1056
Glu Glu Gly Phe Glu Glu Arg Thr Lys Asp Arg Gly Leu Leu Ile Arg           
            340                 345                 350                   

ggg tgg gct cca caa gtg cta ata ctg tca cac cct gca gtt ggt gcg       1104
Gly Trp Ala Pro Gln Val Leu Ile Leu Ser His Pro Ala Val Gly Ala           
        355                 360                 365                       

ttt ttg act cac tgt ggt tgg aac tca gct ctc gaa ggg ata tct gcg       1152
Phe Leu Thr His Cys Gly Trp Asn Ser Ala Leu Glu Gly Ile Ser Ala           
    370                 375                 380                           

ggt gtc cct atg gtg acg tgg cct cag ttt caa gaa caa ttt tac aat       1200
Gly Val Pro Met Val Thr Trp Pro Gln Phe Gln Glu Gln Phe Tyr Asn           
385                 390                 395                 400           

gag aag tta ctt gta caa gta tta aga att ggc gtt agt gtt ggc gcg       1248
Glu Lys Leu Leu Val Gln Val Leu Arg Ile Gly Val Ser Val Gly Ala           
                405                 410                 415               

caa aaa gtt gtg cat tgg ggt gaa gaa gaa aag tca gga gtg gta gta       1296
Gln Lys Val Val His Trp Gly Glu Glu Glu Lys Ser Gly Val Val Val           
            420                 425                 430                   

aag agt gag gaa ttt agt aag gct ata gag atg atg atg gaa gat ggg       1344
Lys Ser Glu Glu Phe Ser Lys Ala Ile Glu Met Met Met Glu Asp Gly           
        435                 440                 445                       

aaa gaa agt gaa gac aga aga aag aga gcc aaa gat ctt ggt aag atg       1392
Lys Glu Ser Glu Asp Arg Arg Lys Arg Ala Lys Asp Leu Gly Lys Met           
    450                 455                 460                           

gca aat gaa gca gtt gaa gaa gga gga tct tct cac cgg aat atg aca       1440
Ala Asn Glu Ala Val Glu Glu Gly Gly Ser Ser His Arg Asn Met Thr           
465                 470                 475                 480           

cga tta atc caa gat att agg aac cta tct agt aca agg aat tca agc       1488
Arg Leu Ile Gln Asp Ile Arg Asn Leu Ser Ser Thr Arg Asn Ser Ser           
                485                 490                 495               

taa                                                                   1491


<210>  4
<211>  496
<212>  PRT
<213>  Stevia rebaudiana


<220>
<221>  PEPTIDE
<222>  (1)..(496)

<400>  4

Met Ala Leu Glu Glu Ser Gln Glu Pro Asn Gln Leu His Phe Leu Val 
1               5                   10                  15      


Ile Pro Leu Gly Ser Pro Gly His Tyr Ile Pro Thr Ile Asp Leu Ser 
            20                  25                  30          


Lys Leu Leu Ala Gln His Gly Val Arg Val Thr Ile Val Thr Thr Pro 
        35                  40                  45              


Val Asn Ala Val Arg Phe Gly Ser Ile Leu Asp Gln Ala Ile Gln Ser 
    50                  55                  60                  


Gly Leu Pro Ile Ser Phe Leu Glu Phe Arg Leu Pro Tyr Met Lys Phe 
65                  70                  75                  80  


Asn Ile Pro Glu Gly Cys Glu Cys Leu Asp Asp Val Pro Asn Ile Gly 
                85                  90                  95      


Ser Ala His Asp Leu Phe Leu Ala His Ser Ser Leu Gln Gln Glu Val 
            100                 105                 110         


Glu Glu Tyr Ile Glu Lys Leu Asp His Lys Pro Ser Cys Ile Leu Ser 
        115                 120                 125             


Gly Thr Tyr Leu Leu Trp Thr Ser Glu Thr Ala Lys Lys Phe Gln Ile 
    130                 135                 140                 


Pro Arg Ile Val Phe Asp Gly Met Asn Cys Phe Thr Gln Met Cys Asn 
145                 150                 155                 160 


His Val Leu Tyr Ile Ser Lys Val Tyr Glu Ser Val Ser Glu Ser Glu 
                165                 170                 175     


Ser Phe Val Leu Pro Gly Leu Pro Asp Arg Ile Glu Leu Thr Arg Ser 
            180                 185                 190         


Gln Leu Ser Phe Val Phe Asn Ser Gly Ser Lys Asp Val Lys Asp Phe 
        195                 200                 205             


Ser Glu Lys Leu Arg Val Ser Glu Ser Glu Ala Phe Gly Ile Val Ile 
    210                 215                 220                 


Asn Ser Phe His Glu Leu Glu Gln Glu Tyr Val Glu Ala Tyr Gln Lys 
225                 230                 235                 240 


Val Lys Glu Asp Lys Ala Trp Cys Ile Gly Pro Leu Ser Leu Cys His 
                245                 250                 255     


Lys Asp Val Ser Glu Lys Val Gln Arg Gly Asn Lys Ser Ser Ile Asp 
            260                 265                 270         


Lys Asp Glu Cys Ile Lys Trp Leu Asp Ser Gln Glu Asn Glu Ser Val 
        275                 280                 285             


Ile Tyr Val Cys Leu Gly Ser Ile Ser Arg Leu Glu Pro Ser Gln Leu 
    290                 295                 300                 


Ile Glu Leu Ala Leu Gly Leu Glu Ser Ser Lys Arg Pro Phe Ile Trp 
305                 310                 315                 320 


Val Val Arg Ala Gly His Lys Thr Glu Lys Ile Glu Lys Trp Ile Asp 
                325                 330                 335     


Glu Glu Gly Phe Glu Glu Arg Thr Lys Asp Arg Gly Leu Leu Ile Arg 
            340                 345                 350         


Gly Trp Ala Pro Gln Val Leu Ile Leu Ser His Pro Ala Val Gly Ala 
        355                 360                 365             


Phe Leu Thr His Cys Gly Trp Asn Ser Ala Leu Glu Gly Ile Ser Ala 
    370                 375                 380                 


Gly Val Pro Met Val Thr Trp Pro Gln Phe Gln Glu Gln Phe Tyr Asn 
385                 390                 395                 400 


Glu Lys Leu Leu Val Gln Val Leu Arg Ile Gly Val Ser Val Gly Ala 
                405                 410                 415     


Gln Lys Val Val His Trp Gly Glu Glu Glu Lys Ser Gly Val Val Val 
            420                 425                 430         


Lys Ser Glu Glu Phe Ser Lys Ala Ile Glu Met Met Met Glu Asp Gly 
        435                 440                 445             


Lys Glu Ser Glu Asp Arg Arg Lys Arg Ala Lys Asp Leu Gly Lys Met 
    450                 455                 460                 


Ala Asn Glu Ala Val Glu Glu Gly Gly Ser Ser His Arg Asn Met Thr 
465                 470                 475                 480 


Arg Leu Ile Gln Asp Ile Arg Asn Leu Ser Ser Thr Arg Asn Ser Ser 
                485                 490                 495     


