                         SEQUENCE LISTING

<110>  Board of Regents, The University of Texas System
       The Trustees of Dartmouth College
 
<120>  ENGINEERED CORONAVIRUS SPIKE (S) PROTEIN AND METHODS OF USE 
       THEREOF

<130>  UTFB.P1251WO

<140>  not yet known
<141>  2021-05-28

<150>  US 63/032,502
<151>  2020-05-29

<160>  80    

<170>  PatentIn version 3.5

<210>  1
<211>  1288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WA1 SARS-Cov-2 ectodomain with trimerization domain and tags at the C-terminus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1208)
<223>  WA1 SARS-CoV-2 Ectodomain

<220>
<221>  MISC_FEATURE
<222>  (1209)..(1288)
<223>  Trimerization domain and tags

<400>  1

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Gly Ser  Gly Tyr Ile Pro Glu  Ala Pro Arg 
    1205                 1210                 1215             


Asp Gly  Gln Ala Tyr Val Arg  Lys Asp Gly Glu Trp  Val Leu Leu 
    1220                 1225                 1230             


Ser Thr  Phe Leu Gly Arg Ser  Leu Glu Val Leu Phe  Gln Gly Pro 
    1235                 1240                 1245             


Gly His  His His His His His  His His Ser Ala Trp  Ser His Pro 
    1250                 1255                 1260             


Gln Phe  Glu Lys Gly Gly Gly  Ser Gly Gly Gly Gly  Ser Gly Gly 
    1265                 1270                 1275             


Ser Ala  Trp Ser His Pro Gln  Phe Glu Lys 
    1280                 1285             


<210>  2
<211>  1208
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 ectodomain consensus sequence for wild-type and variants


<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Leu or Phe

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa is Ser or Ile

<220>
<221>  MISC_FEATURE
<222>  (18)..(18)
<223>  Xaa is Leu or Phe

<220>
<221>  MISC_FEATURE
<222>  (19)..(19)
<223>  Xaa is Thr or Arg

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa is Thr or Asn

<220>
<221>  MISC_FEATURE
<222>  (26)..(26)
<223>  Xaa is Pro or Ser

<220>
<221>  MISC_FEATURE
<222>  (52)..(52)
<223>  Xaa is Gln or Arg

<220>
<221>  MISC_FEATURE
<222>  (67)..(67)
<223>  Xaa is Ala or Val

<220>
<221>  MISC_FEATURE
<222>  (69)..(69)
<223>  Xaa is His or absent

<220>
<221>  MISC_FEATURE
<222>  (70)..(70)
<223>  Xaa is Val, Ile, or absent

<220>
<221>  MISC_FEATURE
<222>  (80)..(80)
<223>  Xaa is Asp, Gly, or Ala

<220>
<221>  MISC_FEATURE
<222>  (95)..(95)
<223>  Xaa is Thr or Ile

<220>
<221>  MISC_FEATURE
<222>  (138)..(138)
<223>  Xaa is Asp or Tyr

<220>
<221>  MISC_FEATURE
<222>  (142)..(142)
<223>  Xaa is Gly or Asp

<220>
<221>  MISC_FEATURE
<222>  (144)..(144)
<223>  Xaa is Tyr, Val, or absent

<220>
<221>  MISC_FEATURE
<222>  (152)..(152)
<223>  Xaa is Trp or Cys

<220>
<221>  MISC_FEATURE
<222>  (154)..(154)
<223>  Xaa is Glu or Lys

<220>
<221>  MISC_FEATURE
<222>  (157)..(157)
<223>  Xaa is Phe or Ser

<220>
<221>  MISC_FEATURE
<222>  (190)..(190)
<223>  Xaa is Arg or Ser

<220>
<221>  MISC_FEATURE
<222>  (215)..(215)
<223>  Xaa is Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (242)..(242)
<223>  Xaa is Leu or absent

<220>
<221>  MISC_FEATURE
<222>  (243)..(243)
<223>  Xaa is Ala or absent

<220>
<221>  MISC_FEATURE
<222>  (244)..(244)
<223>  Xaa is Leu or absent

<220>
<221>  MISC_FEATURE
<222>  (253)..(253)
<223>  Xaa is Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (258)..(258)
<223>  Xaa is Trp or Leu

<220>
<221>  MISC_FEATURE
<222>  (417)..(417)
<223>  Xaa is Lys, Asn, or Thr

<220>
<221>  MISC_FEATURE
<222>  (452)..(452)
<223>  Xaa is Leu or Arg

<220>
<221>  MISC_FEATURE
<222>  (478)..(478)
<223>  Xaa is Thr or Lys

<220>
<221>  MISC_FEATURE
<222>  (484)..(484)
<223>  Xaa is Glu, Lys, or Gln

<220>
<221>  MISC_FEATURE
<222>  (501)..(501)
<223>  Xaa is Asn or Tyr

<220>
<221>  MISC_FEATURE
<222>  (570)..(570)
<223>  Xaa is Ala or Asp

<220>
<221>  MISC_FEATURE
<222>  (614)..(614)
<223>  Xaa is Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (655)..(655)
<223>  Xaa is His or Tyr

<220>
<221>  MISC_FEATURE
<222>  (677)..(677)
<223>  Xaa is Gln or His

<220>
<221>  MISC_FEATURE
<222>  (681)..(681)
<223>  Xaa is Pro, His, or Arg

<220>
<221>  MISC_FEATURE
<222>  (701)..(701)
<223>  Xaa is Ala or Val

<220>
<221>  MISC_FEATURE
<222>  (716)..(716)
<223>  Xaa is Thr or Ile

<220>
<221>  MISC_FEATURE
<222>  (791)..(791)
<223>  Xaa is Thr or Ile

<220>
<221>  MISC_FEATURE
<222>  (859)..(859)
<223>  Xaa is Thr or Asn

<220>
<221>  MISC_FEATURE
<222>  (888)..(888)
<223>  Xaa is Phe or Leu

<220>
<221>  MISC_FEATURE
<222>  (950)..(950)
<223>  Xaa is Asp, His, or Asn

<220>
<221>  MISC_FEATURE
<222>  (982)..(982)
<223>  Xaa is Ser or Ala

<220>
<221>  MISC_FEATURE
<222>  (1027)..(1027)
<223>  Xaa is Thr or Ile

<220>
<221>  MISC_FEATURE
<222>  (1118)..(1118)
<223>  Xaa is Asp or His

<220>
<221>  MISC_FEATURE
<222>  (1176)..(1176)
<223>  Xaa is Val or Phe

<400>  2

Met Phe Val Phe Xaa Val Leu Leu Pro Leu Val Ser Xaa Gln Cys Val 
1               5                   10                  15      


Asn Xaa Xaa Xaa Arg Thr Gln Leu Pro Xaa Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Xaa Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Xaa Ile Xaa Xaa Ser Gly Thr Asn Gly Thr Lys Arg Phe Xaa 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Xaa Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Xaa Pro Phe Leu Xaa Val Xaa 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Xaa Met Xaa Ser Glu Xaa Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Xaa Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Xaa Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Xaa Xaa Xaa His Arg Ser Tyr Leu Thr Pro Gly Xaa Ser Ser Ser 
                245                 250                 255     


Gly Xaa Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Xaa Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Xaa Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Xaa Pro Cys 
465                 470                 475                 480 


Asn Gly Val Xaa Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Xaa Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Xaa Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Xaa Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu Xaa Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Xaa Thr Asn Ser Xaa Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Xaa Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Xaa Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Xaa Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Xaa Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Xaa Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Xaa Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Xaa Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Xaa Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Xaa Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Xaa Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln 
    1205             


<210>  3
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  3
ggtaccaga                                                                9


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  4
ccgtctcagg ccgagttcgg tacc                                              24


<210>  5
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  5
ggaaacaagg caacttcaag aacctgagag aattc                                  35


<210>  6
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  6
gccgtcgatg ttcttgaaca cgaattc                                           27


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  7
gggtctgctt cctctgtagc tagc                                              24


<210>  8
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  8
ggtgtaggcg atgatgctct ggctagc                                           27


<210>  9
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  9
cagctctgtg ctgaacgata tcctgtctag a                                      31


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  10
ggcttctgga gggtccagtc taga                                              24


<210>  11
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
ggcctcgggg atgtatccgg atc                                               23


<210>  12
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  12
gggcaggatc tctgttgtgc agctaattgt aaag                                   34


<210>  13
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  13
ctttacaatt agctgcacaa cagagatcct gccc                                   34


<210>  14
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  14
ggatcttgcc gatgcaagaa ttgaactgg                                         29


<210>  15
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  15
ccagttcaat tcttgcatcg gcaagatcc                                         29


<210>  16
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  16
gccatgcaga tgtgctatag attcaacgga atcggcgtga cctgcaacgt gctgtatg         58


<210>  17
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  17
catacagcac gttgcaggtc acgccgattc cgttgaatct atagcacatc tgcatggc         58


<210>  18
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  18
cttctggagg gtccagtcta gacaggatac agttcagcac agagcagatg gcgccaaaat       60


<210>  19
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  19
cagagatcct gtgcgtgagc atgacc                                            26


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  20
ggtcatgctc acgcacagga tctctg                                            26


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  21
caaactgcag gactgcgtga atcagaacgc                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  22
gcgttctgat tcacgcagtc ctgcagtttg                                        30


<210>  23
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  23
gaccaagacc agctgtgact gtacaatgt                                         29


<210>  24
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  24
acattgtaca gtcacagctg gtcttggtc                                         29


<210>  25
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  25
ttcaacggct gcacagttct cccac                                             25


<210>  26
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  26
gtgggagaac tgtgcagccg ttgaa                                             25


<210>  27
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  27
tcacatgtgg gtggacattt ggcgccggcg cctgcctgca                             40


<210>  28
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  28
tgcaggcagg cgccggcgcc aaatgtccac ccacatgtga                             40


<210>  29
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  29
aggcttagaa ggatcacaga gaatctggct                                        30


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  30
agccagattc tctgtgatcc ttctaagcct                                        30


<210>  31
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  31
tcagtacacc tgtgccctgc tggct                                             25


<210>  32
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  32
agccagcagg gcacaggtgt actga                                             25


<210>  33
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  33
aagcagatct acaagtgccc acctatcaag                                        30


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  34
cttgataggt gggcacttgt agatctgctt                                        30


<210>  35
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  35
tctgccctgc tgtgtggcac catca                                             25


<210>  36
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  36
tgatggtgcc acacagcagg gcaga                                             25


<210>  37
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  37
ctggctaaag ttaaagcagc cgaagtcctt                                        30


<210>  38
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  38
aaggacttcg gctgctttaa ctttagccag                                        30


<210>  39
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  39
aaccagaagc tgatctgtaa ccagttcaat tct                                    33


<210>  40
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  40
agaattgaac tggttacaga tcagcttctg gtt                                    33


<210>  41
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  41
cggccagggt gagcttgttg aac                                               23


<210>  42
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  42
gttcaacaag ctcaccctgg ccg                                               23


<210>  43
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  43
aggccatctg catgaaaaag gggatctg                                          28


<210>  44
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  44
cagatcccct ttttcatgca gatggcct                                          28


<210>  45
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  45
gatcctcgat agggctccgc ttgc                                              24


<210>  46
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  46
gcaagcggag ccctatcgag gatc                                              24


<210>  47
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  47
gacaaaaata ccatggaggt gttcgccc                                          28


<210>  48
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  48
gggcgaacac ctccatggta tttttgtc                                          28


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  49
tcgtcggtcg gcagaggtgg                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  50
ccacctctgc cgaccgacga                                                   20


<210>  51
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  51
gccgcagaag tcaacgaatt tagactgtcc cagc                                   34


<210>  52
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  52
gctgggacag tctaaattcg ttgacttctg cggc                                   34


<210>  53
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  53
ggcttctgga gggtccagtg gagacaggat atcgttc                                37


<210>  54
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  54
gaacgatatc ctgtctccac tggaccctcc agaagcc                                37


<210>  55
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  55
cttcggcttc tggagggtcc ggtctagaca ggatatcg                               38


<210>  56
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  56
cgatatcctg tctagaccgg accctccaga agccgaag                               38


<210>  57
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  57
ggacttcggc ttctggaggg ggcagtctag acagg                                  35


<210>  58
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  58
cctgtctaga ctgccccctc cagaagccga agtcc                                  35


<210>  59
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  59
ggtcttggtc atgctcacgc acaggatctc tgttgtg                                37


<210>  60
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  60
cacaacagag atcctgtgcg tgagcatgac caagacc                                37


<210>  61
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  61
gggcgttctg attcacgcag tcctgcagtt tgccc                                  35


<210>  62
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  62
gggcaaactg caggactgcg tgaatcagaa cgccc                                  35


<210>  63
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  63
cagttacgag tgcgacatcc ctatcggc                                          28


<210>  64
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  64
ccctgaacac cctggtgaag cagc                                              24


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  65
ccctgatcag ctgttgggtc acg                                               23


<210>  66
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  66
cagcacctcc agggatctgc cc                                                22


<210>  67
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  67
ctggttttca tacagcacgt tgcaggtcac gccgattccg ttgaatctat agcacatctg       60

catggcaaag gggatc                                                       76


<210>  68
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  68
gatccccttt gccatgcaga tgtgctatag attcaacgga atcggcgtga cctgcaacgt       60

gctgtatgaa aaccag                                                       76


<210>  69
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  69
gcaggagctc ggcaaatacg agcagggatc                                        30


<210>  70
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  70
gccagaagtc agatgctcaa ggggc                                             25


<210>  71
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  71
cttctcgaac tgggggtggg accaggcgct atgatggtgg tgatg                       45


<210>  72
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  72
tgataatgac tcgagcgata attcactcct caggtgcagg ctgcc                       45


<210>  73
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  73
atgatggtgg tgatggtggt gatggcctgg gc                                     32


<210>  74
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  74
cacaggaaac agctatgacc atgattacgc caagct                                 36


<210>  75
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  75
gatccgcccc ctccactacc tcatcacttc tcgaactggg ggtggg                      46


<210>  76
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  76
cccaccccca gttcgagaag tgatgaggta gtggaggggg cggatc                      46


<210>  77
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  77
ctcgaactgg gggtgggacc atcatcaatg atggtggtga tggtggtg                    48


<210>  78
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  78
caccaccatc accaccatca ttgatgatgg tcccaccccc agttcgag                    48


<210>  79
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  79
cagggcgctg ggggtagagg agagggagtc ctggatcttg                             40


<210>  80
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  80
tcctctaccc ccagcgccct gggcaaac                                          28


