                         SEQUENCE LISTING

<110>  AGILENT TECHNOLOGIES, INC.
 
<120>  ANTI-HUMAN LAG-3 ANTIBODIES AND THEIR USE IN IMMUNOHISTOCHEMISTRY
        (IHC)

<130>  6363.139818PCT/20200046

<140>  to be assigned
<141>  2021-05-26

<150>  63/030,873
<151>  2020-05-27

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  PRT
<213>  homo sapiens

<400>  1

Gly Pro Pro Ala Ala Ala Pro Gly His Pro Leu Ala Pro Gly Pro His 
1               5                   10                  15      


Pro Ala Ala Pro Ser Ser Trp Gly Pro Arg Pro Arg Arg 
            20                  25                  


<210>  2
<211>  112
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  2

Gln Ser Val Lys Glu Ser Glu Gly Gly Leu Phe Lys Pro Thr Asp Thr 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Ile Asp Leu Ser Ser Gly Ile 
            20                  25                  30          


Leu Val Trp Val Arg Gln Ala Pro Gly Ser Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Gly Ile Asp Ala Asn Gly Arg Ala Tyr Tyr Ala Ser Trp Ala Lys Ser 
    50                  55                  60                  


Arg Ser Thr Ile Thr Arg Asn Thr Asn Glu Asn Thr Val Thr Leu Lys 
65                  70                  75                  80  


Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe Cys Ala Gly 
                85                  90                  95      


Gly Ala Trp Asn Ile Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser 
            100                 105                 110         


<210>  3
<211>  113
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  3

Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly 
1               5                   10                  15      


Gly Thr Val Thr Ile Lys Cys Gln Ser Ser Gln Ser Val Tyr Asp Ser 
            20                  25                  30          


Asn Thr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu 
        35                  40                  45              


Leu Met Tyr Ser Ala Ser Thr Leu Ala Phe Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu 
65                  70                  75                  80  


Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Ser Tyr Asp Cys 
                85                  90                  95      


Ser Ser Val Asp Cys Thr Ala Phe Gly Gly Gly Thr Glu Val Val Val 
            100                 105                 110         


Lys 
    


<210>  4
<211>  104
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  4

Gly Asp Pro Gly Ala Pro Thr Val Leu Ile Phe Pro Pro Ala Ala Asp 
1               5                   10                  15      


Gln Val Ala Thr Gly Thr Val Thr Ile Val Cys Val Ala Asn Lys Tyr 
            20                  25                  30          


Phe Pro Asp Val Thr Val Thr Trp Glu Val Asp Gly Thr Thr Gln Thr 
        35                  40                  45              


Thr Gly Ile Glu Asn Ser Lys Thr Pro Gln Asn Ser Ala Asp Cys Thr 
    50                  55                  60                  


Tyr Asn Leu Ser Ser Thr Leu Thr Leu Thr Ser Thr Gln Tyr Asn Ser 
65                  70                  75                  80  


His Lys Glu Tyr Thr Cys Lys Val Thr Gln Gly Thr Thr Ser Val Val 
                85                  90                  95      


Gln Ser Phe Asn Arg Gly Asp Cys 
            100                 


<210>  5
<211>  104
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  5

Gly Asp Pro Val Ala Pro Thr Val Leu Ile Phe Pro Pro Ala Ala Asp 
1               5                   10                  15      


Gln Val Ala Thr Gly Thr Val Thr Ile Val Cys Val Ala Asn Lys Tyr 
            20                  25                  30          


Phe Pro Asp Val Thr Val Thr Trp Glu Val Asp Gly Thr Thr Gln Thr 
        35                  40                  45              


Thr Gly Ile Glu Asn Ser Lys Thr Pro Gln Asn Ser Ala Asp Cys Thr 
    50                  55                  60                  


Tyr Asn Leu Ser Ser Thr Leu Thr Leu Thr Ser Thr Gln Tyr Asn Ser 
65                  70                  75                  80  


His Lys Glu Tyr Thr Cys Lys Val Thr Gln Gly Thr Thr Ser Val Val 
                85                  90                  95      


Gln Ser Phe Asn Arg Gly Asp Cys 
            100                 


<210>  6
<211>  323
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  6

Gly Gln Pro Lys Ala Pro Ser Val Phe Pro Leu Ala Pro Cys Cys Gly 
1               5                   10                  15      


Asp Thr Pro Ser Ser Thr Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
            20                  25                  30          


Leu Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Thr Leu Thr Asn 
        35                  40                  45              


Gly Val Arg Thr Phe Pro Ser Val Arg Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Ser Val Thr Ser Ser Ser Gln Pro Val Thr Cys 
65                  70                  75                  80  


Asn Val Ala His Pro Ala Thr Asn Thr Lys Val Asp Lys Thr Val Ala 
                85                  90                  95      


Pro Ser Thr Cys Ser Lys Pro Thr Cys Pro Pro Pro Glu Leu Leu Gly 
            100                 105                 110         


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
        115                 120                 125             


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
    130                 135                 140                 


Asp Asp Pro Glu Val Gln Phe Thr Trp Tyr Ile Asn Asn Glu Gln Val 
145                 150                 155                 160 


Arg Thr Ala Arg Pro Pro Leu Arg Glu Gln Gln Phe Asn Ser Thr Ile 
                165                 170                 175     


Arg Val Val Ser Thr Leu Pro Ile Ala His Gln Asp Trp Leu Arg Gly 
            180                 185                 190         


Lys Glu Phe Lys Cys Lys Val His Asn Lys Ala Leu Pro Ala Pro Ile 
        195                 200                 205             


Glu Lys Thr Ile Ser Lys Ala Arg Gly Gln Pro Leu Glu Pro Lys Val 
    210                 215                 220                 


Tyr Thr Met Gly Pro Pro Arg Glu Glu Leu Ser Ser Arg Ser Val Ser 
225                 230                 235                 240 


Leu Thr Cys Met Ile Asn Gly Phe Tyr Pro Ser Asp Ile Ser Val Glu 
                245                 250                 255     


Trp Glu Lys Asn Gly Lys Ala Glu Asp Asn Tyr Lys Thr Thr Pro Ala 
            260                 265                 270         


Val Leu Asp Ser Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
        275                 280                 285             


Pro Thr Ser Glu Trp Gln Arg Gly Asp Val Phe Thr Cys Ser Val Met 
    290                 295                 300                 


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Arg Ser 
305                 310                 315                 320 


Pro Gly Lys 
            


<210>  7
<211>  217
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  7

Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly 
1               5                   10                  15      


Gly Thr Val Thr Ile Lys Cys Gln Ser Ser Gln Ser Val Tyr Asp Ser 
            20                  25                  30          


Asn Thr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu 
        35                  40                  45              


Leu Met Tyr Ser Ala Ser Thr Leu Ala Phe Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu 
65                  70                  75                  80  


Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Ser Tyr Asp Cys 
                85                  90                  95      


Ser Ser Val Asp Cys Thr Ala Phe Gly Gly Gly Thr Glu Val Val Val 
            100                 105                 110         


Lys Gly Asp Pro Gly Ala Pro Thr Val Leu Ile Phe Pro Pro Ala Ala 
        115                 120                 125             


Asp Gln Val Ala Thr Gly Thr Val Thr Ile Val Cys Val Ala Asn Lys 
    130                 135                 140                 


Tyr Phe Pro Asp Val Thr Val Thr Trp Glu Val Asp Gly Thr Thr Gln 
145                 150                 155                 160 


Thr Thr Gly Ile Glu Asn Ser Lys Thr Pro Gln Asn Ser Ala Asp Cys 
                165                 170                 175     


Thr Tyr Asn Leu Ser Ser Thr Leu Thr Leu Thr Ser Thr Gln Tyr Asn 
            180                 185                 190         


Ser His Lys Glu Tyr Thr Cys Lys Val Thr Gln Gly Thr Thr Ser Val 
        195                 200                 205             


Val Gln Ser Phe Asn Arg Gly Asp Cys 
    210                 215         


<210>  8
<211>  217
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  8

Ala Gln Val Leu Thr Gln Thr Pro Ser Pro Val Ser Ala Ala Val Gly 
1               5                   10                  15      


Gly Thr Val Thr Ile Lys Cys Gln Ser Ser Gln Ser Val Tyr Asp Ser 
            20                  25                  30          


Asn Thr Leu Ala Trp Phe Gln Gln Lys Pro Gly Gln Pro Pro Lys Leu 
        35                  40                  45              


Leu Met Tyr Ser Ala Ser Thr Leu Ala Phe Gly Val Pro Ser Arg Phe 
    50                  55                  60                  


Ser Gly Ser Gly Ser Gly Thr Gln Phe Thr Leu Thr Ile Ser Asp Leu 
65                  70                  75                  80  


Glu Cys Ala Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Ser Tyr Asp Cys 
                85                  90                  95      


Ser Ser Val Asp Cys Thr Ala Phe Gly Gly Gly Thr Glu Val Val Val 
            100                 105                 110         


Lys Gly Asp Pro Val Ala Pro Thr Val Leu Ile Phe Pro Pro Ala Ala 
        115                 120                 125             


Asp Gln Val Ala Thr Gly Thr Val Thr Ile Val Cys Val Ala Asn Lys 
    130                 135                 140                 


Tyr Phe Pro Asp Val Thr Val Thr Trp Glu Val Asp Gly Thr Thr Gln 
145                 150                 155                 160 


Thr Thr Gly Ile Glu Asn Ser Lys Thr Pro Gln Asn Ser Ala Asp Cys 
                165                 170                 175     


Thr Tyr Asn Leu Ser Ser Thr Leu Thr Leu Thr Ser Thr Gln Tyr Asn 
            180                 185                 190         


Ser His Lys Glu Tyr Thr Cys Lys Val Thr Gln Gly Thr Thr Ser Val 
        195                 200                 205             


Val Gln Ser Phe Asn Arg Gly Asp Cys 
    210                 215         


<210>  9
<211>  435
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  9

Gln Ser Val Lys Glu Ser Glu Gly Gly Leu Phe Lys Pro Thr Asp Thr 
1               5                   10                  15      


Leu Thr Leu Thr Cys Thr Val Ser Gly Ile Asp Leu Ser Ser Gly Ile 
            20                  25                  30          


Leu Val Trp Val Arg Gln Ala Pro Gly Ser Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Gly Ile Asp Ala Asn Gly Arg Ala Tyr Tyr Ala Ser Trp Ala Lys Ser 
    50                  55                  60                  


Arg Ser Thr Ile Thr Arg Asn Thr Asn Glu Asn Thr Val Thr Leu Lys 
65                  70                  75                  80  


Met Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe Cys Ala Gly 
                85                  90                  95      


Gly Ala Trp Asn Ile Trp Gly Pro Gly Thr Leu Val Thr Val Ser Ser 
            100                 105                 110         


Gly Gln Pro Lys Ala Pro Ser Val Phe Pro Leu Ala Pro Cys Cys Gly 
        115                 120                 125             


Asp Thr Pro Ser Ser Thr Val Thr Leu Gly Cys Leu Val Lys Gly Tyr 
    130                 135                 140                 


Leu Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Thr Leu Thr Asn 
145                 150                 155                 160 


Gly Val Arg Thr Phe Pro Ser Val Arg Gln Ser Ser Gly Leu Tyr Ser 
                165                 170                 175     


Leu Ser Ser Val Val Ser Val Thr Ser Ser Ser Gln Pro Val Thr Cys 
            180                 185                 190         


Asn Val Ala His Pro Ala Thr Asn Thr Lys Val Asp Lys Thr Val Ala 
        195                 200                 205             


Pro Ser Thr Cys Ser Lys Pro Thr Cys Pro Pro Pro Glu Leu Leu Gly 
    210                 215                 220                 


Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
225                 230                 235                 240 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln 
                245                 250                 255     


Asp Asp Pro Glu Val Gln Phe Thr Trp Tyr Ile Asn Asn Glu Gln Val 
            260                 265                 270         


Arg Thr Ala Arg Pro Pro Leu Arg Glu Gln Gln Phe Asn Ser Thr Ile 
        275                 280                 285             


Arg Val Val Ser Thr Leu Pro Ile Ala His Gln Asp Trp Leu Arg Gly 
    290                 295                 300                 


Lys Glu Phe Lys Cys Lys Val His Asn Lys Ala Leu Pro Ala Pro Ile 
305                 310                 315                 320 


Glu Lys Thr Ile Ser Lys Ala Arg Gly Gln Pro Leu Glu Pro Lys Val 
                325                 330                 335     


Tyr Thr Met Gly Pro Pro Arg Glu Glu Leu Ser Ser Arg Ser Val Ser 
            340                 345                 350         


Leu Thr Cys Met Ile Asn Gly Phe Tyr Pro Ser Asp Ile Ser Val Glu 
        355                 360                 365             


Trp Glu Lys Asn Gly Lys Ala Glu Asp Asn Tyr Lys Thr Thr Pro Ala 
    370                 375                 380                 


Val Leu Asp Ser Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Ser Val 
385                 390                 395                 400 


Pro Thr Ser Glu Trp Gln Arg Gly Asp Val Phe Thr Cys Ser Val Met 
                405                 410                 415     


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Ile Ser Arg Ser 
            420                 425                 430         


Pro Gly Lys 
        435 


<210>  10
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  10

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  11
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  synthetic polypeptide

<400>  11

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


